Immunolabeling instrument convenient for antibody addition

Abstract

The immunolabeling instrument comprises an immunolabeling instrument body, a mechanical arm and an agent adding assembly, the immunolabeling instrument body, the mechanical arm and the agent adding assembly are all installed on the upper end face of a shell, the mechanical arm is installed on one side of the immunolabeling instrument body, and the agent adding assembly is installed on the other side of the immunolabeling instrument body. An agent adding assembly is installed on the side, away from the immunolabeling instrument body, of the mechanical arm and comprises a lifting mechanism and a placement table, and a bearing is arranged on the contact face of the placement table and the shell. According to the immunolabeling instrument convenient to add the antibody, the medicament adding device is separated from the immunolabeling instrument body, then a reagent bottle is automatically moved between the medicament adding device and the immunolabeling instrument body through the mechanical arm, and then the function of conveniently adding the antibody is achieved; antibodies can be added into one group, markers can be added into the other group, multi-functionalization is achieved, manual intervention is conducted in the process, and then the accuracy of the measurement result is ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of immune labeling equipment, in particular to an immune labeling instrument which is convenient for adding antibodies. Background Art

[0002] Immunolabeling instrument is an instrument used for immunolabeling technology to detect antigen and antibody reactions. Its core function is to detect the reaction of the marker. Commonly used markers in immunolabeling technology include fluorescein, enzymes, radionuclides, and colloidal gold. The working principle of the immunolabeling instrument is to reflect the reaction of antigen and antibody by detecting the activity of the marker, reaction specificity, substrate reaction amplification, storage stability of enzyme-labeled reagents, and ease of operation.

[0003] For example, application number CN202120947638.1 discloses an automatic cell immunolabeling instrument that can complete cell labeling operations on more slide samples at one time, with higher cell labeling efficiency, which greatly meets the usage requirements; the immunolabeling technology is a technology that combines antigen-antibody reaction with labeling technology, labels known antigens or antibodies with markers, and indirectly determines the antigen-antibody complex by detecting the markers. Therefore, when measuring the test object, it is necessary not only to add markers that need to label antigens and antibodies to the immunolabeling instrument, but also to facilitate the addition of antibodies or antigens to the immunolabeling instrument, and then through comparative experiments, further ensure the accuracy of the measurement results.

[0004] Therefore, in view of this, the existing structure and defects are studied and improved, and an immune labeling instrument that is convenient for adding antibodies is proposed to achieve a more practical purpose. Utility Model Content

[0005] The purpose of the utility model is to provide an immune labeling instrument that is convenient for adding antibodies, so as to solve the problem of convenient addition of antibodies raised in the above background technology.

[0006] In order to achieve the above-mentioned purpose, the present invention provides the following technical solutions: an immunolabeling instrument for convenient antibody addition, comprising an immunolabeling instrument body, a mechanical arm and an additive assembly, wherein the immunolabeling instrument body, the mechanical arm and the additive assembly are all mounted on the upper end face of the shell, a mechanical arm is mounted on one side of the immunolabeling instrument body, and an additive assembly is mounted on the side of the mechanical arm away from the immunolabeling instrument body, the additive assembly comprises a lifting mechanism and a placement table, the lifting mechanism comprises a support column and a fixed plate, the support column is mounted on the upper end face of the shell, and the support column is provided in four groups, a fixed plate is provided at one end of the four groups of support columns away from the shell, and an electric extension is provided at the center of the bottom end face of the fixed plate. The retracting rod is provided with an additive mechanism at one end of the electric telescopic rod away from the fixed plate, and the additive mechanism includes a first limit plate and a damper, the first limit plate is installed at the telescopic end of the electric telescopic rod, and the bottom end surface of the first limit plate is provided with a damper, the bottom end surface of the damper is provided with a second limit plate, and both sides of the first limit plate and the second limit plate are provided with injection fixed blocks, and the contact surface of the injection fixed block and the first limit plate and the second limit plate is provided with a slide groove, and the bottom end surface of the second limit plate is dug with a guide groove, a micro-injection pump is longitudinally provided inside the injection fixed block, and a medicine connection port is provided at the top of the micro-injection pump, and an injection port is provided at the bottom end of the micro-injection pump.

[0007] Furthermore, the placing table is embedded in the upper end surface of the shell, and the contact surface between the placing table and the shell is provided with a bearing, a guide block is longitudinally provided at the center of the upper end surface of the placing table, and support blocks are provided on both sides of the guide block, the upper end surface of the placing table is dug with a holding groove, and reset telescopic rods are horizontally provided on both sides of the holding groove, the bottom end surface of the placing table is fixedly connected to the transmission rod, and the outer diameter surface of the transmission rod is provided with a first bevel gear, the first bevel gear is meshedly connected with the second bevel gear, and the second bevel gear is installed at the output end of the servo motor.

[0008] Furthermore, the first limiting plate and the second limiting plate form an up-and-down sliding structure through a sliding groove, the medicine connecting port is threadedly connected to a medicine bottle, and a reagent bottle is placed inside the containing groove.

[0009] Furthermore, eleven groups of holding slots are provided, and the holding slots are evenly arranged with the center of the placement table as the origin. The holding slots are located directly below the injection port, and two groups of injection ports are respectively in the same vertical plane with two groups of holding slots.

[0010] Furthermore, the first bevel gear and the second bevel gear form a gear meshing structure, and the servo motor forms a gear transmission structure with the transmission rod through the first bevel gear and the second bevel gear.

[0011] Furthermore, the placement platform is fixedly connected to the inner diameter surface of the bearing, and the outer diameter surface of the bearing is fixedly connected to the housing. The placement platform forms a rotating structure that rotates around the center of the bearing through the bearing and the housing.

[0012] Compared with the prior art, the beneficial effects of the present invention are as follows: the immunolabeling instrument that facilitates antibody addition realizes the function of convenient antibody addition by separating the reagent addition device from the immunolabeling instrument body, and then automatically moving the reagent bottle between the reagent addition device and the immunolabeling instrument body by a robotic arm. Moreover, due to the provision of two sets of microinjection pumps, one set can be used to add antibodies and the other set to add markers, thereby realizing multifunctionality, and the above processes are all manually intervened, thereby ensuring the accuracy of the measurement results.

[0013] When the immunolabeling instrument convenient for antibody addition is used, when it is necessary to add antibodies or markers to the inner cavity of the reagent bottle, the mechanical arm is started, and the mechanical arm clamps the reagent bottle in the immunolabeling instrument body to the placement table in the additive assembly, so that the reagent bottle falls into the holding tank, and then the reagent bottle is limited by resetting the telescopic rod, thereby ensuring that the reagent bottle remains in an upright state, so that antibodies or markers can be added to the inner cavity of the reagent bottle, and then the electric telescopic rod is started, which drives the first limiting plate to move downward, drives the additive mechanism to move downward, and makes the injection port located directly above the reagent bottle. , wherein when the adding mechanism moves downward, the second limiting plate first contacts the support block. At this time, since a damper is provided between the first limiting plate and the second limiting plate, and since the first limiting plate and the second limiting plate form an up and down sliding structure through the sliding groove, the first limiting plate slides, thereby preventing the second limiting plate from rigidly contacting the placement table, thereby causing damage to the placement table, and a medicine bottle is installed in advance to the medicine connection port at one end of the micro-injection pump, and the addition amount of the antibody or marker is controlled by the micro-injection pump, thereby completing the function of automatically adding the antibody or marker;

[0014] The immunolabeling instrument, which is convenient for antibody addition, starts a servo motor during addition, and the servo motor forms a gear transmission structure with a first bevel gear and a second bevel gear and a transmission rod, and the placement table forms a rotating structure that rotates around the bearing center with a bearing and a housing, thereby rotating the placement table so as to add antibodies or markers to different reagent bottles. In addition, in order to prevent the lifting mechanism from being misplaced or offset when moving up and down, a guide groove and a guide block are used to further ensure that the adding mechanism is located directly above the center of the holding tank so that antibodies or markers can be added to the reagent bottles. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the front view of an immunolabeling instrument that facilitates antibody addition in the present invention;

[0016] Figure 2This is a side view schematic diagram of the structure of an immunolabeling instrument that facilitates antibody addition in the present invention;

[0017] Figure 3 This is a front view structural diagram of an immunolabeling instrument adding mechanism for convenient antibody addition in the present invention;

[0018] Figure 4 This is a schematic diagram of the top view of the structure of an immunolabeling instrument that is convenient for adding antibodies in the present invention.

[0019] In the figure: 1. Immunolabeling instrument body; 2. Robotic arm; 3. Additive assembly; 31. Lifting mechanism; 3101. Support column; 3102. Fixed plate; 3103. Electric telescopic rod; 3104. Additive mechanism; 3105. First limit plate; 3106. Damper; 3107. Second limit plate; 3108. Injection fixed block; 3109. Slide; 31010. Guide groove; 31011. Microinjection pump; 31012. Drug connection port; 31013. Injection port; 32. Placement table; 33. Bearing; 34. Guide block; 35. Support block; 36. Holding tank; 37. Reset telescopic rod; 38. Transmission rod; 39. First bevel gear; 310. Second bevel gear; 311. Servo motor; 4. Housing. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0021] See also Figures 1 to 4The utility model provides a technical solution: an immunolabeling instrument that is convenient for adding antibodies, comprising an immunolabeling instrument body 1, a robotic arm 2 and an additive assembly 3, the immunolabeling instrument body 1, the robotic arm 2 and the additive assembly 3 are all installed on the upper end surface of a shell 4, a robotic arm 2 is installed on one side of the immunolabeling instrument body 1, the robotic arm 2 is specifically a grasping robot of model NIRYO-ONE or other robots with grasping function, the immunolabeling instrument body 1 is specifically a Luminex200 / 200I immunolabeling instrument or other immunolabeling immunolabeling instrument, and an additive assembly 3 is installed on the side of the robotic arm 2 away from the immunolabeling instrument body 1, the additive assembly 3 comprises a lifting mechanism 31 and a placement table 32, the lifting mechanism 31 comprises a support column 3101 and a fixed plate 3102, the support column 3101 is installed on the upper end surface of the shell 4, and the support column 3101 is provided with four groups, and a fixed plate 3102 is provided at the end of the four groups of support columns 3101 away from the shell 4, and the bottom end surface of the fixed plate 3102 An electric telescopic rod 3103 is provided at the center of the electric telescopic rod 3103, and an additive mechanism 3104 is provided at one end of the electric telescopic rod 3103 away from the fixed plate 3102, and the additive mechanism 3104 includes a first limit plate 3105 and a damper 3106, the first limit plate 3105 is installed at the telescopic end of the electric telescopic rod 3103, and the damper 3106 is provided on the bottom end surface of the first limit plate 3105, and the second limit plate 3107 is provided on the bottom end surface of the damper 3106, and the first limit plate 3105 and the second limit plate Both sides of 3107 are provided with injection fixed blocks 3108, and the contact surfaces of the injection fixed blocks 3108 with the first limiting plate 3105 and the second limiting plate 3107 are provided with slide grooves 3109. The bottom end surface of the second limiting plate 3107 is excavated with a guide groove 31010. A micro-injection pump 31011 is longitudinally arranged inside the injection fixed block 3108, and a drug connection port 31012 is provided at the top end of the micro-injection pump 31011, and an injection port 31013 is provided at the bottom end of the micro-injection pump 31011;

[0022] When it is necessary to add antibodies or markers to the inner cavity of the reagent bottle, the robotic arm 2 is started, and the robotic arm 2 clamps the reagent bottle in the immunolabeling instrument body 1 onto the placement table 32 in the additive assembly 3, so that the reagent bottle falls into the holding groove 36, and then the reset telescopic rod 37 is used to limit the reagent bottle to ensure that the reagent bottle remains in an upright state, so as to add antibodies or markers to the inner cavity of the reagent bottle, and then the electric telescopic rod 3103 is started, and the electric telescopic rod 3103 drives the first limiting plate 3105 to move downward, so as to drive the additive mechanism 3104 to move downward, so that the injection port 31013 is located directly above the reagent bottle, wherein when the additive mechanism 3104 moves downward, the second limiting plate 3105 is driven by the electric telescopic rod 3103 to move downward. The positioning plate 3107 first contacts the support block 35. At this time, since a damper 3106 is provided between the first limiting plate 3105 and the second limiting plate 3107, and since the first limiting plate 3105 and the second limiting plate 3107 form an up and down sliding structure using a slide groove 3109, the first limiting plate 3105 slides, thereby preventing the second limiting plate 3107 from rigidly contacting the placement table 32, thereby preventing the placement table 32 from being damaged. In addition, a medicine bottle is installed in advance at the medicine connection port 31012 at one end of the micro-injection pump 31011, and the micro-injection pump 31011 is used to control the amount of antibody or marker added, thereby completing the function of automatically adding antibodies or markers.

[0023] The placing table 32 is embedded in the upper end surface of the shell 4, and the contact surface of the placing table 32 and the shell 4 is provided with a bearing 33. A guide block 34 is longitudinally provided at the center of the upper end surface of the placing table 32, and support blocks 35 are provided on both sides of the guide block 34. A holding groove 36 is dug on the upper end surface of the placing table 32, and reset telescopic rods 37 are horizontally provided on both sides of the holding groove 36. The bottom end surface of the placing table 32 is fixedly connected to a transmission rod 38, and the outer diameter surface of the transmission rod 38 is provided with a first bevel gear 39, the first bevel gear 39 is meshed and connected with a second bevel gear 310, and the second bevel gear 310 is installed on the output of the servo motor 311. At the end, the servo motor 311 is started. The servo motor 311 uses the first bevel gear 39 and the second bevel gear 310 and the transmission rod 38 to form a gear transmission structure, and the placement table 32 uses the bearing 33 and the housing 4 to form a rotating structure that rotates around the center of the bearing 33, so that the placement table 32 rotates to add antibodies or markers to different reagent bottles. In order to prevent the lifting mechanism 31 from being misplaced or offset when moving up and down, the guide groove 31010 and the guide block 34 further ensure that the adding mechanism 3104 is located directly above the center of the holding tank 36, so that antibodies or markers can be added to the reagent bottles;

[0024] The first limiting plate 3105 and the second limiting plate 3107 form an up and down sliding structure through the slide groove 3109, the medicine connection port 31012 is threadedly connected to the medicine bottle, and a reagent bottle is placed on the inner side of the holding groove 36. There are eleven groups of holding grooves 36, and the holding grooves 36 are evenly arranged with the center of the placement platform 32 as the origin. The holding grooves 36 are located directly below the injection port 31013, and the two groups of injection ports 31013 are respectively in the same vertical plane with two groups of holding grooves 36. The first bevel gear 39 and the second bevel gear 310 form a gear meshing structure, and the servo motor 311 forms a gear transmission structure with the transmission rod 38 through the first bevel gear 39 and the second bevel gear 310. The placement platform 32 is fixedly connected to the inner diameter surface of the bearing 33, and the outer diameter surface of the bearing 33 is fixedly connected to the shell 4. The placement platform 32 forms a rotating structure that rotates around the center of the bearing 33 with the shell 4 through the bearing 33.

[0025] Working principle: When using an immunolabeling instrument that is convenient for adding antibodies, when it is necessary to add antibodies or markers to the inner cavity of the reagent bottle, start the robotic arm 2, which clamps the reagent bottle in the immunolabeling instrument body 1 onto the placement table 32 in the additive assembly 3, so that the reagent bottle falls into the holding groove 36, and then uses the reset telescopic rod 37 to limit the reagent bottle to ensure that the reagent bottle remains in an upright state so that antibodies or markers can be added to the inner cavity of the reagent bottle, and then start the electric telescopic rod 3103, which drives the first limiting plate 3105 to move downward, and drives the additive mechanism 3104 to move downward, so that the injection port 31013 is located directly above the reagent bottle, wherein when the additive mechanism 3104 moves downward, the second limiting plate 3107 first contacts the support block 35, at this time A damper 3106 is arranged between the first limit plate 3105 and the second limit plate 3107, and since the first limit plate 3105 and the second limit plate 3107 are formed with an up and down sliding structure using a slide groove 3109, the first limit plate 3105 slides, and the medicine bottle is installed in advance to the medicine connection port 31012 at one end of the microinjection pump 31011. When the microinjection pump 31011 is used to control the amount of antibody or marker added, the servo motor 311 is started, and the servo motor 311 uses the first bevel gear 39 and the second bevel gear 310 and the transmission rod 38 to form a gear transmission structure, and the placement table 32 forms a rotating structure that rotates around the center of the bearing 33 through the bearing 33 and the shell 4, so that the placement table 32 rotates to complete the function of adding antibodies or markers.

Claims

1. An immunolabeling instrument for convenient antibody addition, comprising an immunolabeling instrument body (1), a robotic arm (2) and an additive assembly (3), characterized in that: The immune marker body (1), the mechanical arm (2) and the additive assembly (3) are all mounted on the upper end surface of the housing (4); the mechanical arm (2) is mounted on one side of the immune marker body (1), and the additive assembly (3) is mounted on the side of the mechanical arm (2) away from the immune marker body (1); the additive assembly (3) includes a lifting mechanism (31) and a placement table (32); the lifting mechanism (31) includes a support column (3101) and a fixing plate (3102); the support column (3101) is installed on the upper end surface of the shell (4), and four groups of support columns (3101) are provided. The ends of the four groups of support columns (3101) away from the shell (4) are provided with a fixed plate (3102), and the center of the bottom end surface of the fixed plate (3102) is provided with an electric telescopic rod (3103), and the end of the electric telescopic rod (3103) away from the fixed plate (3102) is provided with an additive mechanism (3104), and the additive mechanism (3104) includes a first limit plate (3101). 105) and a damper (3106), the first limiting plate (3105) is installed at the telescopic end of the electric telescopic rod (3103), and the bottom end surface of the first limiting plate (3105) is provided with a damper (3106), the bottom end surface of the damper (3106) is provided with a second limiting plate (3107), and both sides of the first limiting plate (3105) and the second limiting plate (3107) are provided with a liquid injection fixed block (3108), and the liquid injection fixed block (3108) is connected to the first limiting plate (3105). The contact surfaces of the limiting plate (3105) and the second limiting plate (3107) are provided with a slide groove (3109), the bottom end surface of the second limiting plate (3107) is provided with a guide groove (31010), a micro-injection pump (31011) is longitudinally provided inside the injection fixed block (3108), and a drug connection port (31012) is provided at the top end of the micro-injection pump (31011), and an injection port (31013) is provided at the bottom end of the micro-injection pump (31011).

2. The immunolabeling instrument for facilitating antibody addition according to claim 1, characterized in that: The placing platform (32) is embedded in the upper end surface of the shell (4), and a bearing (33) is provided on the contact surface between the placing platform (32) and the shell (4). A guide block (34) is longitudinally provided at the center of the upper end surface of the placing platform (32), and support blocks (35) are provided on both sides of the guide block (34). A holding groove (36) is dug on the upper end surface of the placing platform (32), and reset telescopic rods (37) are transversely provided on both sides of the holding groove (36). The bottom end surface of the placing platform (32) is fixedly connected to a transmission rod (38), and a first bevel gear (39) is provided on the outer diameter surface of the transmission rod (38). The first bevel gear (39) is meshedly connected to a second bevel gear (310), and the second bevel gear (310) is installed at the output end of the servo motor (311).

3. The immunolabeling instrument for facilitating antibody addition according to claim 2, characterized in that: The first limiting plate (3105) and the second limiting plate (3107) form an up and down sliding structure through a sliding groove (3109), the medicine connection port (31012) is threadedly connected to a medicine bottle, and a reagent bottle is placed inside the containing groove (36).

4. The immunolabeling instrument for facilitating antibody addition according to claim 3, characterized in that: Eleven groups of the containing grooves (36) are provided, and the containing grooves (36) are evenly arranged with the center of the placement table (32) as the origin. The containing grooves (36) are located directly below the injection port (31013), and two groups of injection ports (31013) are respectively in the same vertical plane with two groups of the containing grooves (36).

5. The immunolabeling instrument for facilitating antibody addition according to claim 4, characterized in that: The first bevel gear (39) and the second bevel gear (310) form a gear meshing structure, and the servo motor (311) forms a gear transmission structure with the transmission rod (38) through the first bevel gear (39) and the second bevel gear (310).

6. The immunolabeling instrument for facilitating antibody addition according to claim 5, characterized in that: The placement platform (32) is fixedly connected to the inner diameter surface of the bearing (33), and the outer diameter surface of the bearing (33) is fixedly connected to the housing (4). The placement platform (32) forms a rotating structure that rotates around the center of the bearing (33) through the bearing (33) and the housing (4).

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