Single cell separation device based on single cell sequencing technology

Abstract

The utility model discloses a single cell separation device based on a single cell sequencing technology, which relates to the technical field of single cell separation devices and comprises an operation table, a first protective cover is mounted on one side of the top of the operation table, and a second protective cover is mounted on the other side of the top of the operation table. According to the utility model, through the arrangement of the microscope, the micromanipulation instrument, the dilution cup and the like, large microorganism individuals extracted by the capillary tube can be separated under the action of the microscope, and a single microorganism cell can be picked by taking a microneedle, a hook, a ring and the like from the drawer under the action of the micromanipulation instrument, so that the purpose of separation is achieved; a solution with microorganisms can be diluted through the dilution cup to prepare small liquid drops, and the liquid drops only containing one cell are observed and separated through a microscope, so that the separation purpose is achieved, and the device is high in functionality and rich in unicellular organism separation means.

Description

Technical Field

[0001] The utility model relates to the technical field of single cell separation devices, and in particular to a single cell separation device based on single cell sequencing technology. Background Art

[0002] With the development of cell engineering and the progress of the times, one of the main steps of single-cell sequencing is to separate cells to obtain DNA chains belonging to only one single cell. Common methods for single-cell separation now include capillary separation, micromanipulation, droplet separation, etc. Each separation method has its own advantages and disadvantages.

[0003] Existing single-cell separation devices can only perform single separation work on larger microorganisms or relatively small microorganisms during use, which makes the devices limited. Utility Model Content

[0004] The purpose of the present utility model is to solve the problem that the existing single-cell separation device can only perform single separation work on larger microorganisms or relatively small microorganisms during use, which makes the device have limitations. A single-cell separation device based on single-cell sequencing technology is proposed.

[0005] In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0006] A single-cell separation device based on single-cell sequencing technology, comprising:

[0007] An operating table is provided, wherein a first protective cover is installed on one side of the top of the operating table, and a second protective cover is installed on the other side of the top of the operating table, a dilution cup is placed in the front of the top of the operating table, and a drawer is movably connected to the front of the operating table, a microscope is placed under the first protective cover, and the microscope and the operating table are movably connected, a micromanipulator is placed under the second protective cover, and the micromanipulator and the operating table are movably connected, the bottoms of the micromanipulator and the microscope are connected to a gear column, and the outer side of the gear column is meshed with a gear rod, one end of the gear rod is connected to a pull-out cross bar, and the outer side of the gear rod is connected to a connecting plate, the inner side of the connecting plate is provided with a reserved hole, and the top of the operating table is movably connected to a movable rod.

[0008] Preferably, support rods are connected around the bottom end of the operating table.

[0009] Preferably, the first protective cover and the second protective cover are both connected to the operating table via hinges.

[0010] Preferably, a slot matching the movable rod is provided on the inner side of the drawer.

[0011] Preferably, the movable rod is symmetrical about the central axis of the operating table, and the movable rod forms an elastic structure with the operating table through a spring.

[0012] Preferably, the pulling cross rod forms a transmission structure through a gear rod and a gear column.

[0013] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0014] 1. In the present invention, by providing a microscope, a micromanipulator, a dilution cup, etc., the function of the microscope can be used to separate larger microorganisms extracted by capillary tubes. The function of the micromanipulator can be used to pick up single microbial cells by taking out a microneedle, hook, or ring from a drawer, thereby achieving the purpose of separation. In addition, the solution containing microorganisms can be diluted through the dilution cup to prepare small droplets, and then combined with microscope observation, the droplets containing only one cell can be separated, thereby achieving the purpose of separation. This device has strong functionality and rich means for separating single-cell organisms. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the overall three-dimensional structure of the utility model;

[0016] Figure 2 This is a schematic diagram of the back structure of the operating table in the utility model;

[0017] Figure 3 This is an enlarged structural diagram of point A in the utility model;

[0018] Figure 4 This is a schematic diagram of the distribution structure of the gear rod and the pull-out cross bar in the utility model.

[0019] Legend:

[0020] 1. Operating table; 2. Support rod; 3. First protective cover; 4. Second protective cover; 5. Dilution cup; 6. Drawer; 7. Micromanipulator; 8. Microscope; 9. Movable rod; 10. Gear column; 11. Gear rod; 12. Connecting plate; 13. Reserved hole; 14. Pull-out cross bar; 15. Hinge. DETAILED DESCRIPTION

[0021] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0022] Reference Figure 1-4A single-cell separation device based on single-cell sequencing technology includes an operating table 1, a first protective cover 3 is installed on one side of the top of the operating table 1, and a second protective cover 4 is installed on the other side of the top of the operating table 1. The first protective cover 3 and the second protective cover 4 are connected to the operating table 1 through a hinge 15. By setting the first protective cover 3 and the second protective cover 4, they can respectively protect a microscope 8 and a micromanipulator 7. Support rods 2 are connected to the four sides of the bottom end of the operating table 1 to support the operating table 1.

[0023] A dilution cup 5 is placed at the top front of the operating table 1, and a drawer 6 is movably connected to the front of the operating table 1. A microscope 8 is placed under the first protective cover 3, and the microscope 8 is movably connected to the operating table 1. A micromanipulator 7 is placed under the second protective cover 4, and the micromanipulator 7 is movably connected to the operating table 1. The bottoms of the micromanipulator 7 and the microscope 8 are connected to a gear column 10, and the outer side of the gear column 10 is meshed with a gear rod 11. One end of the gear rod 11 is connected to a pull-out cross bar 14, and the outer side of the gear rod 11 is connected to a connecting plate 12. The pull-out cross bar 14 forms a transmission structure with the gear rod 11 and the gear column 10. When the pull-out cross bar 14 is manually pulled, it pulls the gear rod 11 to move, thereby driving the gear column 10 to rotate, so that the angles of the microscope 8 and the micromanipulator 7 can be adjusted, which is convenient. People use this device to perform single-cell separation work. A reserved hole 13 is provided on the inner side of the connecting plate 12. A movable rod 9 is movably connected to the top of the operating table 1. A slot matching the movable rod 9 is provided on the inner side of the drawer 6. After the movable rod 9 is subjected to the pressure of the protective cover, it moves downward and is inserted into the reserved hole 13 at the connecting plate 12 and the slot at the drawer 6, making it difficult for the drawer 6 to open and the connecting plate 12 to move. The movable rod 9 is symmetrical about the central axis of the operating table 1, and the movable rod 9 forms an elastic structure with the operating table 1 through a spring. When the movable rod 9 is not pressed by an external force, it is reset under the action of the spring, thereby disengaging from the reserved hole 13 at the connecting plate 12 and the slot at the drawer 6, so that the drawer 6 can be opened and the connecting plate 12 can move under the action of external force.

[0024] Working principle: When in use, first rotate the first protective cover 3 and the second protective cover 4 around the hinge 15 to complete the opening operation. At this time, the movable rod 9 is not pressed by external force, and it is reset under the action of the spring, thereby disengaging from the reserved hole 13 at the connecting plate 12 and the slot hole at the drawer 6, so that people can pull the drawer 6 to take the tools placed inside it, and then manually pull the pull-out cross bar 14 to make it pull the tooth rod 11 and the connecting plate 12 to move, thereby driving the tooth column 10 to rotate, so that the angles of the microscope 8 and the micromanipulator 7 can be adjusted, which is convenient for people to perform single cell separation work through the device.

[0025] The above is only a preferred specific implementation method of the present invention, but the protection scope of the present invention is not limited to this. Any technician familiar with the technical field within the technical scope disclosed by the present invention can make equivalent replacements or changes based on the technical solution and utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A single-cell isolation device based on single-cell sequencing technology, comprising: An operating table (1) is characterized in that: a first protective cover (3) is installed on one side of the top of the operating table (1), and a second protective cover (4) is installed on the other side of the top of the operating table (1); a dilution cup (5) is placed in front of the top of the operating table (1), and a drawer (6) is movably connected to the front of the operating table (1); a microscope (8) is placed below the first protective cover (3), and the microscope (8) and the operating table (1) are movably connected; a micromanipulator is placed below the second protective cover (4). The micromanipulator (7) and the operating table (1) are movably connected. The bottoms of the micromanipulator (7) and the microscope (8) are both connected with tooth columns (10), and the outer side of the tooth column (10) is meshedly connected with a tooth rod (11), one end of the tooth rod (11) is connected with a pull-out cross bar (14), and the outer side of the tooth rod (11) is connected with a connecting plate (12), the inner side of the connecting plate (12) is provided with a reserved hole (13), and the top of the operating table (1) is movably connected with a movable rod (9).

2. The single-cell isolation device based on single-cell sequencing technology according to claim 1, characterized in that: Support rods (2) are connected around the bottom end of the operating platform (1).

3. The single-cell isolation device based on single-cell sequencing technology according to claim 1, characterized in that: The first protective cover (3) and the second protective cover (4) are both connected to the operating table (1) via hinges (15).

4. The single-cell isolation device based on single-cell sequencing technology according to claim 1, characterized in that: A slot hole matching the movable rod (9) is provided on the inner side of the drawer (6).

5. The single-cell isolation device based on single-cell sequencing technology according to claim 1, characterized in that: The movable rod (9) is symmetrical about the central axis of the operating table (1), and the movable rod (9) forms an elastic structure with the operating table (1) through a spring.

6. The single-cell isolation device based on single-cell sequencing technology according to claim 1, characterized in that: The pulling crossbar (14) forms a transmission structure through the gear rod (11) and the gear column (10).

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