Experimental device for obtaining microorganism induced mineral flakes

Abstract

The utility model discloses an experimental device for obtaining microorganism induced mineral flakes, and relates to the technical field of mineral flake identification. The device comprises a culture solution conveying mechanism, an experiment monitoring mechanism and a mineral analysis mechanism, the culture solution conveying mechanism comprises a guide pipe, a detachable sealing cover is arranged at the top of the guide pipe, a rubber plug is arranged in the middle of the guide pipe, the experiment monitoring mechanism comprises a pH meter and a conductivity meter probe, the mineral analysis mechanism comprises a cavity and a slide, and the cavity is connected to the bottom of the guide pipe. The cavity comprises a cylindrical cavity body, a plurality of clamping grooves are uniformly formed in the surface of the cylindrical cavity body in the longitudinal direction at intervals, a corresponding slide is inserted into each clamping groove, a plurality of small holes are formed in the surface of the cylindrical cavity body between the adjacent clamping grooves, and the small holes are used for circulating a culture solution inside and outside the cavity body. The device disclosed by the utility model can be used for quickly and conveniently obtaining the mineral flakes induced by in-situ microorganisms for analysis, test and research.

Description

Technical Field

[0001] The utility model relates to the technical field of mineral slice identification, in particular to an experimental device for obtaining microbial-induced mineral slices. Background Art

[0002] Microbial carbonate deposition is a hot topic in current international geological research. It focuses on elucidating the mechanisms of microbial carbonate formation and their role in diagenesis, and on studying the impact of microbial mediation on regional and global biogeochemical cycles of carbon and other elements. Microbial carbonate deposition, a branch of geological microbiology, is the formation of carbonate sediments through the growth and metabolism of microorganisms. The study of microbial carbonate deposition not only provides insights into biological evolution but also facilitates the extraction of chemical elemental indicators from microbial carbonate deposits that effectively reflect climate and environmental changes, thereby reconstructing paleoclimate and paleoenvironmental data. Furthermore, microorganisms play a significant role in the formation of petroleum, travertine, and calcite. Biomound structures within microbial carbonate deposits serve as excellent reservoirs and ore-bearing bodies for lead and zinc ores. Furthermore, microbial carbonate deposits themselves are valuable mineral resources and ore-bearing rock bodies, possessing significant economic significance. Over the past two decades, microbial sedimentation and its environmental significance have been a rapidly developing research field. Advances in microbial geology and geochemistry have fundamentally reshaped core concepts in Earth science. The study of microbial carbonate deposition is of great value in elucidating the role of microbial sedimentation in reefs and mounds, reshaping the environment and climate change, identifying the relationship between microbial community prosperity and biological events during periods of major geological change, studying microbial-induced mineral precipitation, evaluating the oil and gas prospecting of marine carbonate sedimentary formations, and searching for marine natural gas hydrates. The study of microbial carbonate deposition involves the intersection of multiple disciplines, promoting the integration of related disciplines such as geomicrobiology, sedimentology, paleontology, petroleum geology, geochemistry, and (paleo)climate and environment, while also exploring the research significance and application value of these related disciplines from a new perspective.

[0003] In the existing scientific analysis and testing of microbial carbonate deposition, the study of microbially induced minerals mainly involves first detaching the minerals through ultrasonic vibration and oxidant cleaning, and then collecting the minerals for research. This step-by-step operation often causes sample contamination, loss, and distortion of the sample structure.

[0004] This shows that the prior art needs to be further improved. Utility Model Content

[0005] The purpose of the utility model is to provide an experimental device for obtaining microbial-induced mineral slices, which can quickly and conveniently obtain in-situ microbial-induced mineral slices for analysis, testing and research.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] An experimental device for obtaining microbial-induced mineral slices, comprising a culture solution delivery mechanism, an experimental monitoring mechanism, and a mineral analysis mechanism, and also comprising a bottle body used in conjunction with the experimental device;

[0008] The culture fluid delivery mechanism includes a conduit, a detachable sealing cover is provided on the top of the conduit, a rubber stopper is provided in the middle of the conduit, and the rubber stopper is matched with the bottle mouth of the bottle body;

[0009] The experimental monitoring mechanism includes a pH meter and a conductivity meter probe, which are inserted into the catheter from the top to monitor the pH and conductivity of the culture solution;

[0010] The mineral analysis mechanism includes a chamber and a glass slide. The chamber is connected to the bottom of the catheter. The chamber includes a cylindrical cavity. Several slots are evenly spaced in the longitudinal direction of the surface of the cylindrical cavity. A corresponding glass slide is inserted into each slot. Several small holes are provided on the surface of the cylindrical cavity between adjacent slots. The small holes are used for the circulation of culture medium inside and outside the chamber.

[0011] In the above-mentioned experimental device for obtaining microbial-induced mineral slices, the diameter of the tube mouth of the catheter is larger than the outer diameter of the pH meter and conductivity meter probes; the experimental monitoring mechanism also includes a pipette, through which the culture fluid is drawn from the catheter for measurement.

[0012] In the above-mentioned experimental device for obtaining microbial-induced mineral slices, the bottom of the conduit is provided with threads, and the chamber and the bottom of the conduit are connected by threads.

[0013] In the above-mentioned experimental device for obtaining microbial-induced mineral slices, the aperture of the small holes on the surface of the cylindrical cavity is 1 mm, and the width of each slot is different, so as to insert glass slides of different sizes and thicknesses.

[0014] In the above-mentioned experimental device for obtaining microbial-induced mineral slices, the glass slide is a glass slide used in a polarizing microscope or a grooved glass slide used in an X-ray diffractometer.

[0015] In the above-mentioned experimental device for obtaining microbial-induced mineral slices, the catheter is made of PVC material, and the shape and size of the rubber stopper are compatible with the shape and size of the bottle mouth of the bottle body.

[0016] The experimental setup for obtaining microbial-induced mineral slices described above uses a pipette to draw culture fluid from a catheter to measure Ca 2+ Mg 2+ 、CO3 2- 、HCO3 - 、SO4 2- , pH or alkalinity.

[0017] Compared with the prior art, the present invention brings the following beneficial technical effects:

[0018] The utility model proposes an experimental device for obtaining microbial-induced mineral slices, wherein a detachable sealing cover is provided on the top of the conduit. When anaerobic bacteria are cultured, the sealing cover can be closed to isolate the air. When aerobic or facultative anaerobic bacteria are cultured, the sealing cover can be removed and replaced with a sealing film. Such a design can meet the experimental needs.

[0019] In the experimental device of the present invention, a chamber is connected to the bottom of the catheter. The overall shape of the chamber is cylindrical, including a cylindrical cavity. Card slots are evenly spaced longitudinally on the circumferential surface of the chamber. By inserting glass slides of the same or different sizes into each card slot, when the culture fluid enters the cylindrical cavity from the catheter, more mineral particles will be formed on the glass slide. After the mineral particles are collected, the catheter is taken out together with the cylindrical cavity. After the glass slide is removed, in-situ mineral analysis can be directly performed using a polarizing microscope, an X-ray diffractometer, a scanning electron microscope, etc.

[0020] In the experimental device of the utility model, the pH meter and the conductivity meter probe are inserted into the catheter from the top of the catheter to monitor the pH and conductivity of the culture solution, and can measure Ca 2+ Mg 2+ 、CO3 2- 、HCO3 - 、SO4 2- , pH or alkalinity and other parameters.

[0021] The device of the utility model is helpful to directly study the morphology, structure and composition characteristics of in-situ minerals formed in the process of biological induced mineralization, and avoids the disadvantages of sample loss and characteristic distortion caused by the secondary sample preparation process of analytical testing experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The present invention will be further described below with reference to the accompanying drawings:

[0023] Figure 1This is a schematic diagram of the structure of an experimental device for obtaining microbial-induced mineral slices according to the present invention;

[0024] Figure 2 This is a schematic diagram of the experimental device of the utility model in use;

[0025] In the picture:

[0026] 1. Catheter, 2. Chamber, 3. Sealing cap, 4. Rubber stopper, 5. Card slot, 6. Glass slide, 7. Small hole. DETAILED DESCRIPTION

[0027] The present invention proposes an experimental device for obtaining microbial-induced mineral slices. In order to make the advantages and technical solutions of the present invention clearer and more specific, the present invention is further described below in conjunction with specific embodiments.

[0028] An experimental device for obtaining microbial-induced mineral slices comprises a culture solution delivery mechanism, an experimental monitoring mechanism, a mineral analysis mechanism and a bottle body used in conjunction with the experimental device; for example, the commonly used bottle body is a conical bottle body.

[0029] The culture fluid delivery mechanism includes a catheter, a detachable sealing cap 3 is provided at the top of the catheter 1, a rubber stopper 4 is provided in the middle of the catheter, the rubber stopper is matched with the bottle mouth of the bottle body, the opening diameter of the rubber stopper 4 is the same as the outer diameter of the catheter 1, ensuring that no pores are generated to cause contamination by bacteria, and the size of the rubber stopper 4 is replaceable to adapt to conical flasks of different calibers; the function of the culture fluid delivery mechanism is to add culture fluid, reagents, etc. into the conical flask. The specific adding method is to open the sealing cap at the top of the catheter, add culture fluid from the top of the catheter, flow along the inside of the catheter to the bottom of the catheter, and enter the chamber 2 connected to the bottom of the catheter, and finally enter the conical flask. Preferably, the catheter is made of PVC material, and the shape and size of the rubber stopper are adapted to the shape and size of the bottle mouth of the bottle body.

[0030] The experimental monitoring mechanism includes a pH meter, a conductivity meter probe and a pipette. The pH meter and conductivity meter probe are inserted into the catheter from the top of the catheter to complete the monitoring of the pH and conductivity of the culture solution; the culture solution is sucked from the catheter by the pipette for measurement, and the pipette sucks the culture solution from the catheter to measure Ca 2+ Mg 2+ 、CO3 2- 、HCO3 - 、SO4 2- , pH or alkalinity.

[0031] The structures and operating principles of the above-mentioned pH meter, conductivity meter probe and pipette can be obtained by those skilled in the art by referring to the existing technology and will not be described in detail herein.

[0032] The mineral analysis mechanism includes a chamber 2 and a glass slide 6. The chamber is connected to the bottom of the catheter. The chamber includes a cylindrical cavity. The cylindrical cavity structure can facilitate the insertion of more glass slides. Several card slots 5 are evenly spaced in the longitudinal direction of the surface of the cylindrical cavity. A corresponding glass slide 6 is inserted in each card slot 5. Several small holes 7 are provided on the surface of the cylindrical cavity between adjacent card slots. The small holes are used for the circulation of culture medium inside and outside the chamber to facilitate sampling and analysis. The distribution of the small holes can be evenly distributed. The aperture of the small holes is preferably 1 mm. The width of each card slot is different, which is used to insert glass slides of different sizes and thicknesses, such as optical microscope slides, X-ray diffractometer sample slots, etc.

[0033] Preferably, a thread is provided at the bottom of the conduit, and the chamber and the bottom of the conduit are connected via the thread.

[0034] Preferably, the glass slide is a glass slide used for a polarizing microscope or a grooved glass slide for an X-ray diffractometer.

[0035] The following is a detailed description of the method for using the above-mentioned experimental device for obtaining microbial-induced mineral slices.

[0036] The method for using the above-mentioned experimental device for obtaining microbial-induced mineral slices specifically includes the following steps:

[0037] The first step, preparing for the experiment, is to disassemble the device, thoroughly clean it, and sterilize it in an autoclave at 120°C for 30 minutes. After assembling the device with the conical flask, culture medium and seed solution are added through the catheter. Culture solutions with varying Mg / Ca ratios, salinity, alkalinity, and pH values are added to the conical flask through the catheter. If necessary, different microbial species can be inoculated to induce microbial mineralization.

[0038] The second step is the experimental monitoring stage. Open the sealing cover of the catheter and insert the pH meter and conductivity meter probe into the culture medium to monitor the pH value and conductivity. At the same time, use a pipette to take liquid through the catheter to measure the OD value and Ca of the bacterial solution. 2+ Mg 2+ 、CO3 2- 、HCO3 - 、SO4 2- , pH, alkalinity and other parameters;

[0039] The third step is the mineral analysis stage. When a large number of mineral particles are formed on the glass slide, the device is taken out of the conical flask. After removing the glass slide, the in-situ mineral analysis and observation are carried out using a polarizing microscope, X-ray diffractometer, scanning electron microscope, etc.

[0040] Parts not described in the present invention can be realized by adopting or drawing on existing technologies.

[0041] Of course, the above description is not a limitation of the present invention, and the present invention is not limited to the above examples. Changes, modifications, additions or substitutions made by technicians in this technical field within the essential scope of the present invention should also fall within the scope of protection of the present invention.

Claims

1. An experimental device for obtaining microbial-induced mineral slices, comprising a culture fluid delivery mechanism, an experimental monitoring mechanism, and a mineral analysis mechanism, characterized in that: Also included is a bottle body used in conjunction with the experimental device; The culture fluid delivery mechanism includes a conduit, a detachable sealing cover is provided on the top of the conduit, a rubber stopper is provided in the middle of the conduit, and the rubber stopper is matched with the bottle mouth of the bottle body; The experimental monitoring mechanism includes a pH meter and a conductivity meter probe, which are inserted into the catheter from the top to monitor the pH and conductivity of the culture solution; The mineral analysis mechanism includes a chamber and a glass slide. The chamber is connected to the bottom of the catheter. The chamber includes a cylindrical cavity. Several slots are evenly spaced in the longitudinal direction of the surface of the cylindrical cavity. A corresponding glass slide is inserted into each slot. Several small holes are provided on the surface of the cylindrical cavity between adjacent slots. The small holes are used for the circulation of culture medium inside and outside the chamber.

2. The experimental device for obtaining microbial-induced mineral slices according to claim 1, characterized in that: The diameter of the tube opening of the catheter is larger than the outer diameter of the probes of the pH meter and the conductivity meter; the experimental monitoring mechanism also includes a pipette, through which the culture fluid is sucked from the catheter for measurement.

3. The experimental device for obtaining microbial-induced mineral slices according to claim 1, characterized in that: The bottom of the conduit is provided with a thread, and the chamber and the bottom of the conduit are connected via the thread.

4. The experimental device for obtaining microbial-induced mineral slices according to claim 1, characterized in that: The aperture of the small holes on the surface of the cylindrical cavity is 1 mm, and the width of each slot is different, so as to be used for inserting glass slides of different sizes and thicknesses.

5. The experimental device for obtaining microbial-induced mineral slices according to claim 1, characterized in that: The glass slide is a glass slide used for a polarizing microscope or a glass slide with grooves used for an X-ray diffractometer.

6. The experimental device for obtaining microbial-induced mineral slices according to claim 1, characterized in that: The material of the conduit is PVC, and the shape and size of the rubber stopper are adapted to the shape and size of the bottle mouth of the bottle body.

7. The experimental device for obtaining microbial-induced mineral slices according to claim 2, characterized in that: The Ca was measured by aspirating the culture medium from the catheter with a pipette. 2+ Mg 2+ 、CO3 2- 、HCO3 - 、SO4 2- , pH or alkalinity.

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