Applying, nursing and washing membrane box for western blot

Abstract

The utility model relates to a dressing, nursing and washing membrane box for western blot. The dressing, nursing and washing membrane box comprises a box body, the interaction baffle is arranged in the box body and is connected with the bottom surface of the box body; the interaction baffle comprises a plurality of interaction baffle columns, the interaction baffle columns are fixedly connected, and a gap is formed between every two adjacent interaction baffle columns; and the box cover is arranged above the box body. The interactive baffle comprises the multiple interactive baffle columns, and the gaps are formed between the interactive baffle columns, so that washing liquid can flow among the multiple interactive baffles in an interactive mode, the operation of changing and discarding the liquid is completed at a time, the washing liquid is effectively saved, and in the vibration cleaning process, the washing liquid can be replaced and discarded along with vibration. The friction position between the edge of the washing film and the interaction baffle column changes, so that the probability of mutual friction between the edge of the washing film and the interaction baffle column is reduced, the integrity of the edge of the washing film is protected, and the edge of the washing film is prevented from being damaged.

Description

Technical Field

[0001] The invention relates to the technical field of biological experimental devices, in particular to a membrane-washing box for protein immunoblotting. Background Art

[0002] In molecular biology experiments, protein immunoblotting, also known as Western blotting in English, has become an indispensable experimental technique in the field of biological experimental equipment. The daily biological experiments in both universities and research institutes are inseparable from the extensive use of this technology.

[0003] However, the prior art generally uses a larger incubation box for simultaneous incubation, which carries the risk of affecting the experimental results due to the overlapping and mutual friction of the washed membranes. In order to avoid overlapping washed membranes, incubation boxes with baffles are often used to place each washed membrane in a different area. However, in the existing incubation boxes with adjustable baffle plug-ins, during the vibration cleaning process, there is continuous friction between the baffle and the washed membrane, which can easily cause damage to the edge of the washed membrane. In addition, during use, the baffle plug-ins that are not interconnected need to discard and replace the liquid separately, which is cumbersome to operate and results in a large amount of waste of washing liquid.

[0004] In order to solve the above technical problems, the present application provides a membrane-washing box for protein immunoblotting. Summary of the Invention

[0005] In view of the above-mentioned shortcomings and deficiencies of the prior art, the utility model provides an incubation and washing box for protein immunoblotting, thereby solving the technical problems in the prior art that the incubation box with adjustable baffle plug-in is cumbersome to operate when adjusting the baffle plug-in, the baffle plug-in is easily damaged, and the non-interconnected washing baffle intervals need to discard and replace the liquid separately during use, which is cumbersome to operate and causes a large amount of washing liquid to be wasted.

[0006] In order to achieve the above-mentioned purpose, the utility model provides a film-washing box for protein immunoblotting, and the technical solution adopted includes: a box body; an interactive baffle, which is arranged inside the box body and connected to the bottom surface of the box body; the interactive baffle includes a plurality of interactive baffle columns, and the plurality of interactive baffle columns are fixedly connected, and a gap is provided between every two adjacent interactive baffle columns; and a box cover, which is arranged above the box body.

[0007] Optionally, the interactive baffle column includes: a base, which is arranged at the bottom of the box body and connected to the box body; a column, which is arranged above the base and connected to the base; a connecting part, which is arranged above the column and connected to the column; and a top, which is arranged above the connecting part and connected to the connecting part.

[0008] Optionally, the column is a cylindrical structure.

[0009] Optionally, the top portion has a conical structure.

[0010] Optionally, the diameter of the cylinder is smaller than the diameter of the base.

[0011] Optionally, a handle is provided on the side of the box body and connected to the box body.

[0012] The beneficial effects of the utility model are:

[0013] The present invention provides a membrane washing box for protein immunoblotting. The box body is divided into multiple areas by setting interactive baffles, which facilitates the separate washing of multiple membranes. Since the interactive baffles include multiple interactive baffle columns, gaps are set between the interactive baffle columns, which facilitates the interactive flow of washing liquid between the multiple interactive baffles. There is no need to discard or replace the liquid in multiple areas separately. The operation is simple and the liquid replacement and discarding operations are completed at one time, effectively saving washing liquid. In addition, since gaps are set between the interactive baffle columns and the area of ​​the membrane is smaller than the area of ​​the area where the membrane is located, during the vibration cleaning process, the position of friction between the edge of the membrane and the interactive baffle columns will change with the vibration, so that the probability of mutual friction between the edge of the membrane and the interactive baffle columns is reduced, which is conducive to protecting the integrity of the edge of the membrane and avoiding damage to the edge of the membrane. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the overall structure of the membrane application and washing box of the present invention;

[0015] Figure 2 This is a schematic diagram of the internal structure of the membrane-washing box of the present invention from another angle;

[0016] Figure 3 This is a structural diagram of the interactive baffles of the membrane-washing box of the present invention;

[0017] Figure 4 This is a structural schematic diagram of the interactive baffle columns of the membrane application and washing box of the present invention.

[0018] Description of reference numerals:

[0019] 1. Box body; 2. Interactive baffle; 3. Interactive baffle column; 4. Box cover; 5. Base; 6. Column; 7. Connecting part; 8. Top; 9. Handle. DETAILED DESCRIPTION

[0020] To better understand the above technical solution, exemplary embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. Although exemplary embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. Rather, these embodiments are provided to enable a clearer and more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0021] Example:

[0022] The embodiment of the present invention provides a membrane washing box for protein immunoblotting, such as Figure 1 、 Figure 2 、 Figure 3 and Figure 4 As shown, it includes: a box body 1; an interactive baffle 2, which is arranged inside the box body 1 and connected to the bottom surface of the box body 1; the interactive baffle 2 includes a plurality of interactive baffle columns 3, and the plurality of interactive baffle columns 3 are fixedly connected, and a gap is provided between every two adjacent interactive baffle columns 3; a box cover 4, which is arranged above the box body 1.

[0023] For example, since a plurality of interactive baffles 2 are provided in the box body 1, the plurality of interactive baffles 2 divide the space inside the box body 1 into a plurality of relatively independent areas, and a gap is provided between every two adjacent interactive baffle columns 3. During the vibration cleaning process of the membrane wash, since the membrane wash needs to be placed in the separated area and immersed in the washing liquid, the size of the membrane wash is smaller than the separated area, so during vibration cleaning, the position of the membrane wash will change in the separated area. Since the interactive baffle 2 is composed of a plurality of interactive baffle columns 3 connected to each other, and a gap is provided between every two adjacent interactive baffle columns 3. With this arrangement, the edge of the membrane wash will contact and rub against the interactive baffle columns 3. Since the position of the membrane wash will change, the edge of the membrane wash will contact and rub against the interactive baffle columns 3 at different positions, and in the gaps between the interactive baffle columns 3, the edge of the membrane wash will not rub against the interactive baffle columns 3. This differs from the baffles used in the prior art, which, along with the vibration cleaning process, cause the edges of the membrane to repeatedly rub against the baffles. Therefore, the interactive baffles 2 provided within the housing 1 of this embodiment help reduce friction between the edges of the membrane and the interactive baffles 2, improving membrane integrity and preventing damage. Furthermore, the wash solution can flow alternately between the multiple interactive baffles 2, eliminating the need to discard and replace the solution in multiple areas separately. The replacement and discarding operations can be completed simultaneously, effectively conserving wash solution.

[0024] For example, the interactive baffles 2 can be connected to the interior of the box body 1 by plugging, gluing, or snapping. The specific connection method is known in the art and is not specifically limited here. Furthermore, the positions of multiple interactive baffles 2 can be determined based on the actual size of the membrane to be washed, thereby dividing the interior of the box body 1 into different areas to meet the requirements of membrane washing and storage.

[0025] For example, the specific number of interactive baffle columns 3 included in each interactive baffle 2 is not specifically limited here, and the specific number of interactive baffle columns 3 included in each interactive baffle 2 can be determined based on the actual size of the membrane washing and box body 1.

[0026] In one possible embodiment, Figure 3 and Figure 4 As shown, the interactive baffle column 3 includes: a base 5, which is arranged at the bottom of the box body 1 and connected to the box body 1; a column 6, which is arranged above the base 5 and connected to the base 5; a connecting part 7, which is arranged above the column 6 and connected to the column 6; and a top 8, which is arranged above the connecting part 7 and connected to the connecting part 7.

[0027] For example, the base 5 , the column 6 , the connecting portion 7 and the top 8 of the interactive baffle column 3 in this embodiment are all formed in one piece.

[0028] In one possible embodiment, Figure 3 and Figure 4 As shown, the column 6 is a cylindrical structure.

[0029] For example, the column 6 of the interactive baffle column 3 in this embodiment is a cylindrical structure. During the vibration cleaning process, the friction of the washing liquid flowing in the gap between adjacent interactive baffle columns 3 is reduced, which is conducive to increasing the fluidity of the washing liquid, ensuring the cross-flow and interaction of the washing liquid, and increasing the cleaning effect of the washing liquid on the membrane.

[0030] In one possible embodiment, Figure 3 and Figure 4 As shown, the top 8 is a conical structure.

[0031] For example, the top 8 of the interactive baffle column 3 in this embodiment is a conical structure. With this arrangement, during the placement of the washing membrane, even due to improper operation, the washing membrane can still slide along the inclined surface of the conical top 8 to the corresponding area inside the box body 1 by relying on the gravity of the washing membrane itself, avoiding collision and friction between the edge of the washing membrane and the interactive baffle 2, which is beneficial to protecting the integrity of the washing membrane and avoiding damage to the washing membrane.

[0032] In one possible embodiment, Figure 4 As shown, the diameter of the column 6 is smaller than the diameter of the base 5 .

[0033] Exemplarily, the column 6 is integrally formed and connected to the base 5. Since the diameter of the column 6 is smaller than the diameter of the base 5, on the one hand, the gap between two adjacent interactive baffle columns 3 is further increased, which is beneficial to the interaction of the washing liquid inside the box body 1, and there is no need to replace the washing liquid in different areas of the box body 1 respectively; on the other hand, the gap between two adjacent interactive baffle columns 3 is increased, which can further reduce the friction between the washing membrane and the interactive baffle columns 3. Correspondingly, the contact probability between the edge of the washing membrane and the gap between two adjacent interactive baffle columns 3 is increased, thereby avoiding damage to the edge of the washing membrane, effectively protecting the integrity of the washing membrane, and avoiding damage to the washing membrane.

[0034] In one possible embodiment, Figure 1 and Figure 2 As shown, the handle 9 is arranged on the side of the box body 1 and connected to the box body 1.

[0035] Exemplarily, there are two handles 9, which are respectively arranged on both sides of the box body 1, and the handles 9 are integrally formed with the box body 1. By providing the handles 9, it is convenient for the operator to stabilize the box body 1 during the vibration cleaning process.

[0036] In this utility model, unless otherwise specified or limited, the terms "installed," "connected," "connect," "fixed," etc. should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection, electrical connection; direct connection, or indirect connection through an intermediate medium; internal communication between two components, or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on specific circumstances.

[0037] In the present invention, unless otherwise expressly specified or limited, when a first feature is “above” or “below” a second feature, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Moreover, when a first feature is “above,” “above,” or “above” a second feature, it may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is at a higher level than the second feature. When a first feature is “below,” “below,” or “below” a second feature, it may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is at a lower level than the second feature.

[0038] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are illustrative and cannot be understood as limitations on the present invention. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A membrane washing box for protein immunoblotting, characterized in that: include: Box body; An interactive baffle is arranged inside the box body and connected to the bottom surface of the box body; The interactive baffle comprises a plurality of interactive baffle columns, and the plurality of interactive baffle columns are fixedly connected, and a gap is provided between every two adjacent interactive baffle columns; The box cover is arranged above the box body.

2. The membrane-washing box for protein immunoblotting according to claim 1, characterized in that: The interactive baffle column comprises: A base, disposed at the bottom of the box body and connected to the box body; A column is provided above the base and connected to the base; A connecting portion is provided above the column and connected to the column; The top portion is arranged above the connecting portion and connected to the connecting portion.

3. The membrane-washing box for protein immunoblotting according to claim 2, characterized in that: The column is a cylindrical structure.

4. The membrane-washing box for protein immunoblotting according to claim 2, characterized in that: The top is a conical structure.

5. The membrane-washing box for protein immunoblotting according to claim 2, characterized in that: The diameter of the column is smaller than the diameter of the base.

6. The membrane-washing box for protein immunoblotting according to any one of claims 1 to 5, characterized in that: Also includes: The handle is arranged on the side of the box body and connected to the box body.

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