Full-automatic collection device for eDNA samples of freshwater benthonic animals

Abstract

The utility model relates to a full-automatic collection device for an eDNA (Deoxyribose Nucleic Acid) sample of a freshwater benthonic animal, which aims at solving the technical problems that the collection of the eDNA sample of the benthonic animal is time-consuming and labor-consuming and the collection efficiency of the eDNA sample of the benthonic animal is influenced because the eDNA sample of the benthonic animal in water is collected by manually filtering at present. A stand column is fixedly connected to the middle of the bottom end of an inner cavity of the cylinder body, three partition plates are installed between the outer side of the stand column and the inner wall of the cylinder body at equal intervals, and the three partition plates divide the cylinder body into three identical collecting bins; the device comprises a stand column, a driving motor is installed in the middle of the top end of the stand column, a driving gear is installed at the driving end of the driving motor, and electric telescopic rods are installed on the stand column on the two sides of the driving motor. The efficiency of collecting the benthonic animal eDNA sample is further improved.

Description

Technical Field

[0001] The utility model relates to the field of benthic animal eDNA sample collection, in particular to a fully automatic collection device for freshwater benthic animal eDNA samples. Background Art

[0002] Benthic animals are a group of aquatic animals that mostly inhabit the bottom of water bodies. Different benthic animal species have varying adaptability to environmental conditions, tolerance, and sensitivity to adverse factors such as pollution. Therefore, benthic animals are important indicator organisms in aquatic ecosystems. Collecting them using traditional survey methods can, to a certain extent, cause irreversible impacts on these ecosystems. However, eDNA technology can directly extract DNA fragments from environmental samples. Environmental DNA technology is non-invasive, efficient, and convenient, and has been widely used in recent years in biodiversity assessments and endangered species surveys.

[0003] Traditional freshwater benthic animal eDNA samples require personnel to receive them, soak them in alcohol, and then manually filter them to collect the benthic animal eDNA samples in the water. This makes the collection of benthic animal eDNA samples time-consuming and labor-intensive, thus affecting the efficiency of benthic animal eDNA sample collection. Therefore, new technical solutions need to be designed to solve this problem. Utility Model Content

[0004] The purpose of this utility model is to overcome the shortcomings of the existing technology, adapt to actual needs, and provide a fully automatic collection device for freshwater benthic animal eDNA samples to solve the technical problem that the current manual filtering is performed to collect benthic animal eDNA samples in water, which makes the collection of benthic animal eDNA samples time-consuming and labor-intensive, thereby affecting the efficiency of benthic animal eDNA sample collection.

[0005] In order to achieve the purpose of the utility model, the technical solution adopted by the utility model is as follows: a fully automatic eDNA sample collection device for freshwater benthic animals is designed, comprising a cylinder, a column is fixedly connected to the middle of the bottom end of the inner cavity of the cylinder, and three partitions are equidistantly installed between the outer side of the column and the inner wall of the cylinder, and the three partitions divide the cylinder into three identical collection chambers;

[0006] A driving motor is installed in the middle of the top of the column, and a driving gear is installed at the driving end of the driving motor. Electric telescopic rods are installed on the columns on both sides of the driving motor, and a support ring is fixedly connected between the driving ends of the two electric telescopic rods. Three connecting plates are equidistantly installed on the outside of the support ring, and connecting rods are rotatably passed through the two connecting plates through bearings. Driven gears are installed on the tops of the three connecting rods, and mounting shafts are provided at the bottoms of the three connecting rods. Connecting frames are installed at the bottoms of the three connecting frames, and sieve drums are installed at the bottoms of the three sieve drums.

[0007] Preferably, an internal threaded barrel is fixedly connected to the mounting shaft, the connecting rod is a threaded rod, and the outer side of the connecting rod is adapted to the inner side of the internal threaded barrel.

[0008] Preferably, three L-shaped discharge pipes are equidistantly connected to the bottom of the cylinder, the three L-shaped discharge pipes are respectively connected to three collection bins, and valves are installed on the three L-shaped discharge pipes.

[0009] Preferably, three supporting legs are installed at the bottom of the cylinder, and a chassis is fixedly connected between the bottoms of two of the supporting legs.

[0010] Preferably, the maximum telescopic range of the electric telescopic rod is greater than the sum of the lengths of the connection rod and the mounting shaft.

[0011] Preferably, the three driven gears are distributed at equal intervals, and all of the three driven gears are engaged with the driving gear.

[0012] Compared with the prior art, the beneficial effects of the present invention are:

[0013] 1. The utility model combines a cylinder, a column, a partition, an L-shaped discharge pipe, a collection chamber, a drive motor, a driving gear, a driven gear, a connecting ring, a connecting plate, a connecting rod, a mounting shaft and a sieve drum to automatically soak and clean the bottom mud of three different samples at the same time without the need for manual operation, thereby further improving the efficiency of collecting benthic animal eDNA samples.

[0014] 2. The utility model combines the internal threaded barrel and the connecting rod, and by twisting the internal threaded barrel, the mounting shaft and the connecting rod can be disassembled, thereby facilitating the disassembly of the screen barrel, and further facilitating the cleaning or replacement of the screen barrel. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0016] Figure 2 This is a schematic diagram of the internal structure of the cylinder of the utility model;

[0017] Figure 3 This is a schematic diagram of the connection structure between the driven gear and the screen drum of the utility model;

[0018] Figure 4 This is a schematic diagram of the screen drum structure of the present utility model;

[0019] In the figure: 1. Cylinder; 11. Column; 12. Partition; 13. L-shaped discharge pipe; 14. Collection bin; 2. Driving motor; 21. Driving gear; 3. Driven gear; 31. Mounting shaft; 32. Connecting frame; 33. Electric telescopic rod; 34. Support ring; 35. Connecting plate; 36. Connecting rod; 37. Internally threaded cylinder; 38. Screen cylinder; 39. Screen plate; 4. Chassis; 41. Support legs. DETAILED DESCRIPTION

[0020] The present invention is further described below with reference to the accompanying drawings and embodiments:

[0021] Example 1: A fully automatic device for collecting eDNA samples of freshwater benthic animals, see Figures 1 to 4 , comprising a cylinder 1, a column 11 being fixedly connected to the middle of the bottom end of the inner cavity of the cylinder 1, three partitions 12 being equidistantly installed between the outer side of the column 11 and the inner wall of the cylinder 1, the three partitions 12 dividing the cylinder 1 into three identical collection chambers 14;

[0022] A driving motor 2 is installed at the middle of the top of the column 11, and a driving gear 21 is installed at the driving end of the driving motor 2. Electric telescopic rods 33 are installed on the columns 11 on both sides of the driving motor 2. A support ring 34 is fixedly connected between the driving ends of the two electric telescopic rods 33. Three connecting plates 35 are equidistantly installed on the outside of the support ring 34. Connecting rods 36 are rotatably passed through the two connecting plates 35 through bearings. Driven gears 3 are installed on the tops of the three connecting rods 36. The three driven gears 3 are equidistantly distributed, and The three driven gears 3 are all meshed with the driving gear 21, the bottoms of the three connecting rods 36 are all provided with mounting shafts 31, the bottoms of the three mounting shafts 31 are all installed with connecting frames 32, the bottoms of the three connecting frames 32 are all installed with screen drums 38, the bottoms of the three screen drums 38 are all installed with screen plates 39, the bottom of the cylinder 1 is equidistantly connected with three L-shaped discharge pipes 13, the three L-shaped discharge pipes 13 are respectively connected with three collection bins 14, and valves (not shown in the text) are installed on the three L-shaped discharge pipes 13. When working, First, start the electric telescopic rod 33 to push the connecting ring to move upward so that the sieve drum 38 is placed above the cylinder 1, and then the three sample bottom muds collected from the bottom of the water are respectively placed in the sieve drum 38, and then start the electric telescopic rod 33 to pull the connecting ring to move downward so that the sieve drum 38 is placed in the collection chamber 14, and at the same time, the driven gear 3 is engaged with the driving gear 21, and then alcohol is added to the collection box to soak the bottom mud, and at the same time, the driving motor 2 is started to drive the driving gear 21 to rotate, and the driven gear 3 is coordinated to drive the sieve drum 38 to rotate to improve the efficiency and effectiveness of the bottom mud soaking in the sieve drum 38. As a result, when the bottom mud is soaked, the valve on the L-shaped discharge pipe 13 is opened to discharge the alcohol, and then clean water is added to the collection box to clean the bottom mud. At the same time, the drive motor 2 is started to drive the sieve drum 38 to rotate to accelerate the cleaning of the bottom mud. When the cleaning is completed, the electric telescopic rod 33 is started to push the sieve drum 38 to be placed above the cylinder 1 to collect the remaining benthic animals in the sieve drum 38, so that the bottom mud of three different samples can be automatically soaked and cleaned at the same time, without the need for manual operation by personnel, further improving the efficiency of collecting benthic animal eDNA samples.

[0023] For details, see Figure 3 The mounting shaft 31 is fixedly connected to an internal threaded barrel 37, and the connecting rod 36 is a threaded rod. The outer side of the connecting rod 36 is adapted to the inner side of the internal threaded barrel 37, and the direction in which the internal threaded barrel 37 and the connecting rod 36 are screwed is the same as the sieve barrel 38. By screwing the internal threaded barrel 37, it is convenient to disassemble the mounting shaft 31 and the connecting rod 36, and then it is convenient to disassemble the sieve barrel 38, and further facilitate the cleaning or replacement of the sieve barrel 38.

[0024] It is worth noting that, see Figure 1Three supporting legs 41 are installed at the bottom of the cylinder 1, and a chassis 4 is fixedly connected between the bottoms of two of the supporting legs 41 to improve the stability of the cylinder 1.

[0025] It is worth noting that see Figure 3 The maximum telescopic range of the electric telescopic rod 33 is greater than the sum of the lengths of the connection between the connecting rod 36 and the mounting shaft 31, which is used to ensure that the telescopic rod 33 can be extended to place the sieve drum 38 above the cylinder body 1, making it easier to remove benthic animals in the sieve drum 38 and put in bottom mud.

[0026] In addition, the components designed in this utility model are all universal standard parts or components known to technical personnel in this field. Their structures and principles can be known to technical personnel through technical manuals or through conventional experimental methods. They can be fully implemented by technical personnel in this field. Needless to say, the content protected by this utility model does not involve improvements to internal structures and methods.

[0027] The embodiments disclosed in the present invention are preferred embodiments, but are not limited to them. Ordinary technicians in this field can easily understand the spirit of the present invention based on the above embodiments and make different extensions and changes. As long as they do not deviate from the spirit of the present invention, they are all within the scope of protection of the present invention.

Claims

1. A fully automatic device for collecting eDNA samples of freshwater benthic animals, comprising a cylinder (1), characterized in that: A column (11) is fixedly connected to the middle of the bottom end of the inner cavity of the cylinder (1), and three partitions (12) are installed at equal distances between the outer side of the column (11) and the inner wall of the cylinder (1), and the three partitions (12) divide the cylinder (1) into three identical collection chambers (14); A driving motor (2) is installed at the middle of the top of the column (11), and a driving gear (21) is installed at the driving end of the driving motor (2). Electric telescopic rods (33) are installed on the columns (11) on both sides of the driving motor (2). A support ring (34) is fixedly connected between the driving ends of the two electric telescopic rods (33). Three connecting plates (35) are equidistantly installed on the outer side of the support ring (34). Connecting rods (36) are rotatably passed through the two connecting plates (35) through bearings. The tops of the three connecting rods (36) are installed with driven gears (3). The bottoms of the three connecting rods (36) are provided with mounting shafts (31). The bottoms of the three mounting shafts (31) are installed with connecting frames (32). The bottoms of the three connecting frames (32) are installed with sieve drums (38), and the bottoms of the three sieve drums (38) are installed with sieve pans (39).

2. The fully automatic device for collecting eDNA samples of freshwater benthic animals according to claim 1, characterized in that: An internal threaded barrel (37) is fixedly connected to the installation shaft (31), and the connecting rod (36) is a threaded rod. The outer side of the connecting rod (36) is adapted to the inner side of the internal threaded barrel (37).

3. The fully automatic device for collecting eDNA samples of freshwater benthic animals according to claim 1, characterized in that: The bottom of the cylinder (1) is equidistantly connected to three L-shaped discharge pipes (13), which are respectively connected to three collection bins (14), and valves are installed on the three L-shaped discharge pipes (13).

4. The fully automatic device for collecting eDNA samples of freshwater benthic animals according to claim 1, characterized in that: Three supporting legs (41) are installed at the bottom of the cylinder (1), and a chassis (4) is fixedly connected between the bottoms of two of the supporting legs (41).

5. The fully automatic device for collecting eDNA samples of freshwater benthic animals according to claim 1, characterized in that: The maximum telescopic range of the electric telescopic rod (33) is greater than the sum of the lengths of the connection between the connecting rod (36) and the installation shaft (31).

6. The fully automatic device for collecting eDNA samples of freshwater benthic animals according to claim 1, characterized in that: The three driven gears (3) are distributed at equal intervals, and all three driven gears (3) are meshed with the driving gear (21).

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