Needle replacing device and automatic microbial colony selecting device

Abstract

The utility model belongs to the technical field of biopharmaceutical instruments. The needle head replacing device comprises a telescopic gun head, a sleeve, a needle head, a sleeve driving mechanism and a telescopic gun head driving mechanism, the needle head is arranged at the lower end of the telescopic gun head through a buckle structure, the telescopic gun head is connected with the telescopic gun head driving mechanism, the sleeve is slidably arranged outside the telescopic gun head in a sleeving mode, and the length of the telescopic gun head is larger than that of the sleeve. And the sleeve is connected with a sleeve driving mechanism. The telescopic gun head driving mechanism drives the telescopic gun head to move to the designated position, the needle head is installed on the telescopic gun head, after use, the telescopic gun head and the needle head are driven again to move to the designated position, the sleeve driving mechanism drives the sleeve to move downwards to exceed the length of the telescopic gun head, and the lower end of the sleeve abuts against the needle head to separate the needle head from the telescopic gun head. The telescopic gun head is simple in structure, and the needle head and the telescopic gun head can be installed and separated. The automatic microbial colony selection device can quickly and reliably complete large-scale colony observation and selection work with high precision.

Description

Technical Field

[0001] The utility model belongs to the technical field of biopharmaceutical equipment, in particular to a needle replacement device and a microbial colony automatic selection device. Background Art

[0002] Microbial colony picking is widely used in fields such as biology, agronomy, animal husbandry, and veterinary science. This process utilizes a fully automated robotic platform, combined with a high-resolution imaging system, to precisely and quickly pick colonies with disposable pick needles. This allows for reliable and high-precision colony selection on a large scale, resolving the contamination and inevitable errors caused by manual operation that can lead to inaccurate experimental data. However, existing technologies often utilize complex fixed disposable pick needles, making them prone to loose mounting. This can cause the needle to fall off after a period of robotic operation, or remain stuck, leading to unreliable experimental data. Utility Model Content

[0003] The technical problem to be solved by the utility model is to overcome the deficiencies of the prior art and provide a needle replacement device which has a simple structure and can easily realize the installation and removal of the needle.

[0004] The technical solution adopted by the utility model to solve its technical problems is: the needle replacement device includes a telescopic gun head, a sleeve, a needle, a sleeve driving mechanism and a telescopic gun head driving mechanism; the needle is arranged at the lower end of the telescopic gun head through a snap structure; the telescopic gun head is connected to the telescopic gun head driving mechanism; the sleeve is slidably arranged on the outside of the telescopic gun head; the length of the telescopic gun head is greater than the length of the sleeve; the sleeve is connected to the sleeve driving mechanism.

[0005] Preferably, the sleeve driving mechanism includes a fixed frame, an electromagnet, a magnet push rod and a spring, the electromagnet is arranged at the upper end of the fixed frame, the magnet push rod is slidably arranged on the fixed frame and is arranged at the lower end of the electromagnet, the spring is sleeved on one end of the magnet push rod close to the electromagnet, and the other end of the magnet push rod is connected to the sleeve.

[0006] Preferably, the snap-fit ​​structure includes a protrusion and a groove, the protrusion is arranged on the lower side of the telescopic gun head, the groove is arranged on the upper side of the hollow cavity inside the needle head, and the protrusion is arranged in the groove.

[0007] Preferably, the needle includes a dipping needle and a needle tube, the dipping needle is arranged in the needle tube, the diameter of the needle tube away from the dipping needle gradually decreases to form a tapered fixing portion, and the interior of the needle tube is hollow.

[0008] A device for automatically picking microbial colonies includes a three-axis robotic arm, a needle mounting rack, a high-definition camera, a solution well plate mounting rack, a culture dish mounting rack and a needle recovery bucket. The needle replacement device is arranged on the three-axis robotic arm, the solution well plate mounting rack, the culture dish mounting rack and the needle mounting rack are arranged on one side of the three-axis robotic arm in sequence, the solution well plate is arranged on the solution well plate mounting rack, the culture dish is arranged on the culture dish mounting rack, the needle is arranged on the needle mounting rack, the high-definition camera is arranged directly above the culture dish mounting rack, and the needle recovery bucket is arranged on one side of the culture dish mounting rack.

[0009] Preferably, the culture dish mounting frame and the needle mounting frame have the same number of holes.

[0010] Preferably, the high-definition camera is connected to the strain identification system.

[0011] Compared with the existing technology, the beneficial effects of this technical solution are:

[0012] The utility model adopts a needle replacement device, in which the needle is detachably mounted at the lower end of the telescopic gun head, and the sleeve is slidably mounted on the outside of the telescopic gun head. The length of the telescopic gun head is greater than the length of the sleeve. The telescopic gun head driving mechanism drives the telescopic gun head to move to a specified position, and the needle is installed on the telescopic gun head. After use, the telescopic gun head and the needle are again driven to the specified position. The sleeve driving mechanism drives the sleeve downward beyond the length of the telescopic gun head, and the lower end of the sleeve presses against the needle to separate the needle and the telescopic gun head. The utility model has a simple structure and realizes the installation and removal of the needle and the telescopic gun head.

[0013] An automatic microbial colony picking device uses a fully automatic three-axis robotic arm platform combined with a high-resolution imaging system to quickly, reliably, and accurately complete large-scale colony observation and picking tasks, meeting the requirements of high-throughput biological experiments and perfectly resolving inaccurate experimental data caused by contamination and inevitable errors caused by manual operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a structural schematic diagram of a needle replacement device of the present utility model.

[0015] Figure 2 for Figure 1 Enlarged view of point A in the middle.

[0016] Figure 3 This is a front view of an automatic microbial colony selection device of the present utility model.

[0017] Figure 4 This is a top view of an automatic microbial colony selection device of the present utility model.

[0018] Figure 5 This is a side view of an automatic microbial colony selection device of the present invention.

[0019] Among them: 1. telescopic gun head 2, sleeve 3, needle 4, protrusion 5, groove 6, fixing frame 7, electromagnet 8, magnet push rod 9, spring 10, dipping needle 11, three-axis robotic arm 12, needle mounting frame 13, high-definition camera 14, solution well plate mounting frame 15, culture dish mounting frame 16, needle recovery bucket. DETAILED DESCRIPTION

[0020] Figures 1 to 5 This is the best embodiment of the present invention, Figures 1 to 5 The utility model is further described.

[0021] Reference Figures 1 and 2 The needle replacement device includes a telescopic gun head 1, a sleeve 2, a needle 3 and a sleeve driving mechanism. A circle of protrusions 4 are provided along the circumference of the lower side of the telescopic gun head 1, and a circle of grooves 5 are provided along the circumference of the upper side of the hollow cavity inside the needle 3. The protrusions 4 are provided in the grooves 5. The sleeve 2 is slidably sleeved on the outside of the telescopic gun head 1. The length of the telescopic gun head 1 is longer than the length of the sleeve 2. The diameter of the sleeve 2 is the same as the diameter of the needle 3 cavity. The sleeve 2 is connected to the sleeve driving mechanism.

[0022] The sleeve drive mechanism includes a fixed frame 6, an electromagnet 7, a magnet push rod 8, and a spring 9. The fixed frame 6 is a vertical rectangular plate with two horizontally protruding layers, forming a movement space for the magnet push rod 8. The upper layer consists of two left and right positioning blocks, and the lower layer has a semicircular through-hole in the middle. One end of the magnet push rod 8 passes through this through-hole. The spring 9 is mounted on the magnet push rod 8 between the upper and lower layers, and the spring 9 is in a compressed state. The magnet push rod 8 is an L-shaped push rod, tilted to the left and installed on the fixed frame 6. The vertical section has a thick diameter at one end and a thin diameter at the other end. The thin end is connected to the horizontal section push rod. The end of the horizontal section push rod is fixed to the sleeve 2, thereby driving the sleeve 2 up and down. A horizontal rectangular positioning plate is set at the end of the thick diameter section. The length of the positioning plate is longer than the distance between the two positioning blocks. Because the spring 9 is in a compressed state, the positioning plate fits the two positioning blocks.

[0023] The needle 3 includes a dipping needle 10 and a needle tube. A groove 5 is provided at one end of the needle tube, and the diameter of the other end gradually decreases to form a tapered fixing portion. The interior of the needle tube is hollow, and the dipping needle 10 is provided in the needle tube.

[0024] When the electromagnet 7 is powered off, the spring 9 is in a compressed state, pressing the positioning plate against the positioning block, and the sleeve 2 is located at the upper end of the telescopic gun head 1. The telescopic gun head 1 can be inserted into the needle 3 so that the protrusion 4 is engaged in the groove 5, thereby fixing the needle 3. When the electromagnet 7 is powered on, the repelling magnet push rod 8 overcomes the compression force of the spring 9, causing the magnet push rod 8 to slide downward through the through hole on the fixing frame 6, thereby driving the sleeve 2 to slide downward until the lower end of the sleeve 2 contacts the upper end of the needle tube of the needle 3. The sleeve 2 continues to move downward, exerting a downward force on the needle 3, causing the protrusion 4 to fall off the groove 5, and the needle 3 falls off.

[0025] Reference Figures 3-5 The microbial colony picking device using the needle replacement device includes a three-axis robotic arm 11, a needle mounting bracket 12, a high-definition camera 13, a solution well plate mounting plate 14, a culture dish mounting bracket 15 and a needle recovery bucket 16. The telescopic gun head 1 is arranged at the lower end of the three-axis robotic arm 11, and the three-axis robotic arm 11 can move in three directions. The needle mounting bracket 12 is arranged on one side of the three-axis robotic arm 11, and the needle 3 is installed on the needle mounting bracket 12. A culture dish mounting frame 15 and a solution well plate mounting plate 14 are arranged side by side on the same side of the needle mounting frame 12. The needle mounting frame 12 is located on the right side of the culture dish mounting frame 15. The high-intensity camera 13 is located above the culture dish mounting frame 15 to take pictures and identify the culture dishes on the culture dish mounting frame 15. The solution well plate mounting plate 14 is located on the left side of the culture dish mounting frame 15. The needle recovery bucket 16 is arranged on the upper side of the culture dish mounting frame 15. The three-axis robotic arm 11 drives the telescopic gun head 1 and the needle 3 to realize the movement in the three directions of ZYX, thereby completing the selection of colonies.

[0026] Working process:

[0027] The automatic microbial colony picking device first takes a picture of the culture dish on the culture dish mounting rack 15 through a high-definition camera 13, uploads the picture to the system for identification and positioning of the bacterial species, and then drives the telescopic gun head 1 to move to the top of the needle mounting rack 12 through a high-precision three-axis robotic arm 11 and drops it. At this time, the electromagnet 7 is powered off, and the magnet push rod 8 is pushed to the upper end by the spring 9. At this time, the sleeve 2 on the telescopic gun head 1 is at the top, and the needle 3 on the needle mounting rack 12 can be installed on the telescopic gun head 1. Then the three-axis robotic arm 11 drives the needle 3 to move to the top of the colony to be picked, descends and dips the colony , and then it is lifted and moved to the top of the culture well plate on the solution well plate mounting rack 14, and then the needle 3 is lowered to immerse the dipped colony into the solution. After completion, the three-axis robotic arm 11 moves to the top of the trash can. At this time, the electromagnet 7 is energized, and the magnet push rod 8 is repelled and pressed to the lower end by the electromagnet 7, driving the sleeve 3 downward until it exceeds the length of the telescopic gun head 1, and the sleeve 3 hits the upper end of the needle tube, so that the needle 3 is pushed out and falls into the needle recovery bucket 16. The three-axis robotic arm 11 continues to move to the top of the needle mounting rack 12, and then descends to automatically install the needle 3, thereby completing one operation.

[0028] The above description is merely a preferred embodiment of the present invention and does not constitute any limitation thereto. Any person skilled in the art may utilize the above disclosure to modify or remodel the present invention into equivalent embodiments. However, any simple modification, equivalent variation, or modification of the above embodiment that does not depart from the technical content of the present invention and is based on the technical essence of the present invention shall still fall within the scope of protection of the present invention.

Claims

1. A needle replacement device, characterized in that: The invention comprises a telescopic gun head (1), a sleeve (2), a needle (3), a sleeve drive mechanism and a telescopic gun head drive mechanism, wherein the needle (3) is arranged at the lower end of the telescopic gun head (1) through a snap-fit ​​structure, the telescopic gun head (1) is connected to the telescopic gun head drive mechanism, the sleeve (2) is slidably sleeved on the outside of the telescopic gun head (1), the length of the telescopic gun head (1) is greater than the length of the sleeve (2), and the sleeve (2) is connected to the sleeve drive mechanism.

2. A needle replacement device according to claim 1, characterized in that: The sleeve driving mechanism comprises a fixing frame (6), an electromagnet (7), a magnet push rod (8) and a spring (9), wherein the electromagnet (7) is arranged at the upper end of the fixing frame (6), the magnet push rod (8) is slidably arranged on the fixing frame (6) and is arranged at the lower end of the electromagnet (7), the spring (9) is sleeved on one end of the magnet push rod (8) close to the electromagnet (7), and the other end of the magnet push rod (8) is connected to the sleeve (2).

3. The needle replacement device according to claim 1, characterized in that: The buckle structure comprises a protrusion (4) and a groove (5), wherein the protrusion (4) is arranged on the lower side of the telescopic gun head (1), and the groove (5) is arranged on the upper side of the hollow cavity inside the needle head (3), and the protrusion (4) is arranged in the groove (5).

4. The needle replacement device according to claim 1, characterized in that: The needle (3) comprises a dipping needle (10) and a needle tube. The dipping needle (10) is arranged in the needle tube. The diameter of one end of the needle tube away from the dipping needle (10) gradually decreases to form a tapered fixing portion. The interior of the needle tube is hollow.

5. An automatic microbial colony selection device using the needle replacement device according to any one of claims 1 to 4, characterized in that: The invention comprises a three-axis robotic arm (11), a needle mounting frame (12), a high-definition camera (13), a solution orifice plate mounting frame (14), a culture dish mounting frame (15) and a needle recovery bucket (16), wherein the needle replacement device is arranged on the three-axis robotic arm (11), the solution orifice plate mounting frame (14), the culture dish mounting frame (15) and the needle mounting frame (12) are arranged on one side of the three-axis robotic arm (11) in sequence, the solution orifice plate is arranged on the solution orifice plate mounting frame (14), the culture dish is arranged on the culture dish mounting frame (15), the needle (3) is arranged on the needle mounting frame (12), the high-definition camera (13) is arranged directly above the culture dish mounting frame (15), and the needle recovery bucket (16) is arranged on one side of the culture dish mounting frame (15).

6. The automatic microbial colony selection device according to claim 5, characterized in that: The number of holes in the solution orifice mounting frame (14) is consistent with the number of holes in the needle mounting frame (12).

7. The automatic microbial colony selection device according to claim 5, characterized in that: The high-definition camera (13) is connected to the bacterial species identification system.

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