Cloning selection module
By designing cloning and selection modules, using racks, industrial cameras and precision mechanical means, the problem of lens restrictions of existing bacterial picking equipment is solved, high-precision colony imaging is achieved, and the accuracy of small colonies is improved.
Patent Information
- Application Number
- CN202422363576.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-27
- Publication Date
- 2025-08-22
- Estimated Expiration
- 2034-09-27
AI Technical Summary
The existing bacteria-picking equipment has limited the lens size and structure due to the camera installation method, so large-volume telecentric lenses and telecentric backlight cannot be used, resulting in the edges of the colony picture being not sharp and it is difficult to correctly interpret small colonies.
A cloning selection module is designed, including a rack, industrial camera, industrial lens, rotary shell picking mechanism and horizontal picking and placement mechanism. Through the cooperation of jaws, electric suction cups and servo motors, precise operation and high-quality imaging of the Petri dish are achieved.
High-precision imaging of colonies is achieved, ensuring that small colonies can be correctly interpreted, and improving the operational convenience and imaging quality of bacteria-picking equipment.
Smart Images

Figure CN223255261U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of bacteria picking equipment, in particular to a clone picking module. Background Art
[0002] Clone picking refers to the process of picking a single colony from an agar plate into a target container, typically a 96-well plate. There are professional colony picking equipment available on the market, as well as colony picking systems that utilize 8-channel workstations with internal robotic arms. However, when imaging the colonies, human intervention can often lead to abnormal bacterial colonies, resulting in deviations in bacterial imaging. Therefore, a clone picking module is needed.
[0003] The application number retrieved is: CN202021181048.4, a bacteria picking device for microbial experiments, wherein a culture dish is provided on the light-transmitting plate, the purpose of which is to overcome the shortcomings of the prior art and provide a bacteria picking device for microbial experiments that is easy to operate and use.
[0004] However, the existing bacteria picking equipment still has certain defects in actual use: the existing bacteria picking equipment places the camera on the bacteria picking arm, which limits the size and structure of the camera, making it impossible to use large telecentric lenses and telecentric backlights. As a result, the edges of the pictures taken of the bacterial colony are not sharp enough, which makes it impossible to correctly interpret the small colonies. For this reason, we propose a clone picking module. Summary of the Invention
[0005] In order to make up for the above deficiencies, the utility model provides a clone picking module, which aims to improve the existing bacteria picking equipment in which the camera is placed on the bacteria picking arm, thereby limiting the size and structure of the camera, resulting in the inability to use large telecentric lenses and telecentric backlights. As a result, the edges of the pictures taken of the bacterial colony are not sharp enough, which will lead to the problem that the colonies cannot be correctly interpreted when they are small.
[0006] The utility model realizes a clone selection module through the following technical scheme, including a frame and an industrial camera, the top of the frame is installed with a top plate by bolts, and the top of the top plate is provided with a fixing hole, and a limit baffle is installed below the fixing hole by bolts, and an industrial lens is provided at the center of the limit baffle and the top plate, and a connecting piece is installed above the industrial lens, and the industrial camera is provided on the side of the connecting piece away from the industrial camera, a layer plate is provided below the top plate, and a rotary shell removal mechanism is provided above the layer plate, and a stacking mechanism is provided on the left side of the rotary shell removal mechanism, and a horizontal pick-up and placement mechanism is provided on the side of the industrial lens away from the rotary shell removal mechanism.
[0007] Furthermore, the stacking mechanism includes a driving motor, a connecting base, a connecting column and a placement bracket, and the driving motor drives the connecting base installed above, and the connecting column is installed above the connecting base, and the placement bracket is installed on the outer surface of the connecting column.
[0008] Furthermore, the rotary shell removal mechanism includes a support base, a horizontal movable slide, an electric suction cup, a mounting bracket and a docking plate, and a horizontal movable slide is installed above the support base, and an electric suction cup is provided above the horizontal movable slide, and the left side of the electric suction cup is connected to the docking plate, and a mounting bracket is provided on the side of the mounting bracket away from the docking plate.
[0009] Furthermore, the horizontal picking and placing mechanism includes a base plate, a clamping jaw, a connecting bracket, a slider, a first servo motor and a second servo motor, and two groups of clamping jaws are placed above the base plate, and a connecting bracket is installed above the two groups of clamping jaws, and a slider is provided on the side of the connecting bracket away from the rotary shell taking component, and a vertical movable slide is provided on the side of the slider away from the connecting bracket, and a first servo motor is installed below the base plate, and a second servo motor is provided below the vertical movable slide.
[0010] Furthermore, a placement table is placed at the center of the rotary shell-taking mechanism and the horizontal taking-and-placing mechanism, and a visual light source is installed below the placement table through bolts.
[0011] Furthermore, the bottom of the layer plate is mounted on a fixed base by bolts, and support blocks are provided below the four groups of fixed bases.
[0012] The utility model provides a clone selection module, which has the following beneficial effects:
[0013] At this time, the gripper in the horizontal pick-and-place mechanism is driven by the vertical moving slide, so that the gripper clamps the culture dish placed on the placement bracket in the stacking mechanism. At this time, the culture dish is placed on the placement table and the cover is removed by the rotary shell removal mechanism. At this time, the industrial camera cooperates with the industrial lens and visual light source to image the bacterial colony inside the culture dish placed on the placement table. After completing the imaging of the culture dish, the rotary cover removal mechanism resets the cover body, and the horizontal pick-and-place mechanism puts the culture dish back into the stacking mechanism. The specific contents are as follows:
[0014] The stacking mechanism includes a driving motor, a connecting base, a connecting column and a placing bracket, and the driving motor drives a connecting base installed above, and a connecting column is installed above the connecting base, and a placing bracket is installed on the outer surface of the connecting column, the rotating shell taking mechanism includes a supporting base, a horizontally movable slide, an electric suction cup, a mounting bracket and a docking plate, and a horizontally movable slide is installed above the supporting base, and an electric suction cup is arranged above the horizontally movable slide, and the left side of the electric suction cup is connected to the docking plate, and a mounting bracket is arranged on the side of the mounting bracket away from the docking plate, the horizontal picking and placing mechanism includes a bottom plate, a clamping jaw, a connecting bracket, a slider, a first servo motor and a second servo motor, and two groups of clamping jaws are placed above the bottom plate, and a connecting bracket is installed above the two groups of clamping jaws, and a slider is arranged on the side of the connecting bracket away from the rotary shell taking assembly, and a vertically movable slide is arranged on the side of the slider away from the connecting bracket, and a first servo motor is installed below the bottom plate, and a second servo motor is arranged below the vertically movable slide. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 This is a schematic diagram of the overall structure of a clone selection module of the present invention;
[0016] Figure 2 This is a schematic diagram of the right side cross-section of a stacking mechanism of a clone selection module of the present invention;
[0017] Figure 3 This is a front view structural diagram of a rotary shell extraction mechanism of a clone selection module of the present invention;
[0018] Figure 4 This is a schematic diagram of the left side cross-section of an industrial lens with a clone selection module of the utility model.
[0019] Figure 5 This is a schematic diagram of the rear cross-section of a vertical movable slide of a clone selection module of the present invention;
[0020] Figure 6 This is a schematic diagram of the structure of an industrial lens of a clone selection module from a top view in the present invention;
[0021] Figure 7 This is a schematic diagram of the structure of a rack of a clone selection module from a top view according to the present invention.
[0022] In the figure: 1. Frame; 2. Industrial camera; 3. Connecting parts; 4. Industrial lens; 5. Limit baffle; 501. Fixing hole; 6. Top plate; 7. Stacking mechanism; 701. Drive motor; 702. Connecting base; 703. Connecting column; 704. Placement bracket; 8. Layer; 9. Fixed base; 901. Support block; 10. Rotary shell removal mechanism; 11. Support base; 12. Horizontal moving slide; 13. Electric suction cup; 14. Mounting bracket; 1401. Docking plate; 15. Placement table; 16. Bottom plate; 17. Clamp; 18. Connecting bracket; 19. Slider; 20. Visual light source; 21. First servo motor; 22. Vertical moving slide; 23. Horizontal pick-and-place mechanism; 24. Second servo motor. DETAILED DESCRIPTION
[0023] The following is a further detailed description of the embodiments of the present invention in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.
[0024] like Figure 1 - Figure 7 As shown, a clone picking module includes a frame 1 and an industrial camera 2. A top plate 6 is installed on the top of the frame 1 by bolts, and a fixing hole 501 is provided at the top of the top plate 6, and a limit baffle 5 is installed below the fixing hole 501 by bolts, and an industrial lens 4 is provided at the center of the limit baffle 5 and the top plate 6, and a connecting member 3 is installed above the industrial lens 4, and the industrial camera 2 is arranged on the side of the connecting member 3 away from the industrial camera 2, a layer plate 8 is provided below the top plate 6, and a rotary shell removal mechanism 10 is provided above the layer plate 8, and a stacking mechanism 7 is provided on the left side of the rotary shell removal mechanism 10, and a horizontal pick-and-place mechanism 23 is provided on the side of the industrial lens 4 away from the rotary shell removal mechanism 10, wherein the industrial camera 2 is fixed on the top plate 6 on the frame 1 in cooperation with the industrial lens 4, and the industrial camera 2 is directly vertically docked on the placement table 15 in cooperation with the industrial lens 4, so that when the culture dish is placed on the top of the placement table 15, the industrial camera 2 can cooperate with the industrial camera 2 to collect bacteria inside the culture dish.
[0025] The stacking mechanism 7 includes a driving motor 701, a connecting base 702, a connecting column 703 and a placement bracket 704. The driving motor 701 drives the connecting base 702 installed above, and the connecting column 703 is installed above the connecting base 702, and the placement bracket 704 is installed on the outer surface of the connecting column 703. The stacking mechanism 7 drives the rotating column to rotate by the driving motor 701, and the connecting base 702 is used to support the rotating column to maintain the stability of the rotating column. At the same time, when the rotating column rotates, the placement bracket 704 placed above the connecting column 703 rotates with the rotating column, so that the culture dish placed inside the placement bracket 704 can be adjusted at any time.
[0026] The rotary shell-removing mechanism 10 includes a supporting base 11, a horizontally movable slide 12, an electric suction cup 13, a mounting bracket 14 and a docking plate 1401. The horizontally movable slide 12 is installed above the supporting base 11, and the electric suction cup 13 is provided above the horizontally movable slide 12. The left side of the electric suction cup 13 is connected to the docking plate 1401, and the mounting bracket 14 is provided on the side of the mounting bracket 14 away from the docking plate 1401. At this time, the horizontal picking and placing mechanism 23 clamps the culture dish in the stacking mechanism 7 to the top of the placement table 15. At this time, the horizontally movable slide 12 drives the electric suction cup 13 to slide. At this time, the mounting bracket 14 drives the electric suction cup 13 to rotate through the docking plate 1401, so that the electric suction cup 13 moves to the top of the culture dish. At this time, the electric suction cup 13 adsorbs the cover of the culture dish. After the adsorption is completed, the motor suction cup resets and takes away the cover of the culture dish, waiting for the subsequent industrial camera 2 to search for bacteria inside the culture dish.
[0027] The horizontal pick-and-place mechanism 23 includes a base plate 16, a clamping jaw 17, a connecting bracket 18, a slider 19, a first servo motor 21 and a second servo motor 24, and two groups of clamping jaws 17 are placed above the base plate 16, and a connecting bracket 18 is installed above the two groups of clamping jaws 17, and a slider 19 is provided on the side of the connecting bracket 18 away from the rotary shell removal assembly, and a vertical moving slide 22 is provided on the side of the slider 19 away from the connecting bracket 18, and a first servo motor 21 is installed below the base plate 16, and a second servo motor 24 is provided below the vertical moving slide 22. The horizontal pick-and-place mechanism 23 is used to accurately grasp and place the culture dish. Above the base plate 16, two groups of clamping jaws 17 are arranged in parallel. These clamping jaws 17 are designed to ensure that the sample in the dish will not be damaged during the movement when clamping the culture dish. The connecting bracket 18 is located above the two groups of clamping jaws 17 and plays a role of connection and support. It integrates the clamping jaws 17 with other moving parts to ensure the stability of the overall mechanism. On the other side of the connecting bracket 18, that is, the side away from the rotary shell removal assembly, a slider 19 is installed. The slider 19 can slide on a specific track, which increases the flexibility of the rotary shell removal mechanism 10 in the horizontal direction. Connected to the slider 19 is a vertical movable slide 22, which allows the rotary shell removal mechanism 10 to move up and down accurately in the vertical direction to adapt to stacking mechanisms 7 of different heights. This enables the stacking mechanism 7 to find culture dishes of different working heights, and the first servo motor 21, which is usually used to drive the horizontal movement of the entire mechanism, ensures that the clamp 17 can accurately reach the culture dish for clamping. The second servo motor 24 below the vertical movable slide 22 is responsible for controlling the lifting and lowering of the slide, achieving precise control of the clamp 17 in the vertical direction, and ensuring that the culture dish can be placed smoothly on the placement table 15.
[0028] A placement table 15 is placed at the center of the rotary shell removal mechanism 10 and the horizontal pick-up and placement mechanism 23. A visual light source 20 is installed under the placement table 15 by bolts. At this time, the horizontal pick-up and placement mechanism 23 places the culture dish on the placement table 15. At this time, the visual light source 20 is started, and the light is gathered through the placement table 15 to pass through the culture dish above the placement table 15. At this time, the bacterial colony inside the culture dish is released by the light source. At this time, when shooting with the industrial camera 2, the industrial camera 2 can better obtain the visual position of the bacterial colony when obtaining the bacterial colony.
[0029] The bottom of the layer plate 8 is fixed to the fixed base 9 by bolts, and support blocks 901 are provided below the four groups of fixed bases 9. The fixed base 9 and the support blocks 901 support the frame 1.
[0030] In summary, the present invention uses the frame 1 and the industrial camera 2. At this time, the culture dishes that have completed bacterial culture are placed on the placement bracket 704 on the stacking mechanism 7 in sequence. At this time, the position of the culture dish can be driven by the stacking mechanism 7 at any time to drive the connecting column 703, so that the connecting column 703 drives the culture dish to rotate, thereby facilitating the placement of the culture dish. At the same time, it is convenient to stack the culture dishes. At this time, the vertical movable slide 22 of the horizontal picking and placing structure drives the clamping claw 17 to move. When the clamping claw 17 moves to the culture dish position at the stacking mechanism 7, the first servo motor 21 drives the clamping claw 17 to clamp the culture dish. After completing the clamping of the culture dish, the vertical movable slide 22 drives the clamping claw 17 to move above the placement table 15. At this time, the clamping claw 17 fixes the culture dish so that the culture dish is placed above the placement table 15. At this time, the horizontal movable slide 12 of the rotary shell taking mechanism 10 drives the electric suction cup 13 to rotate. When it moves to the corresponding position of the placement table 15, the mounting bracket 14 drives the docking plate 1401, so that the docking plate 1401 When the connected electric suction cup 13 moves to the cover body above the culture dish, the electric suction cup 13 absorbs the top cover of the culture dish and peels off the top cover of the culture dish. When the electric suction cup 13 completes taking the top cover of the culture dish, the clamping claw 17 is released and reset by the vertical moving slide 22. The electric suction cup 13 that has taken the bottom cover of the culture dish drives the docking plate 1401 to flip through the mounting bracket 14, and moves through the horizontal moving slide 12 to place the top cover of the culture dish in the designated area below the placement table 15. The visual light source 20 is started, and the light is gathered through the placement table 15 to illuminate the culture dish. At this time, the industrial camera 2 illuminates the bacterial colony inside the culture dish through the industrial lens 4, thereby acquiring the image of the bacterial colony in the culture dish. After the bacterial colony acquisition is completed, when the electric suction cup 13 moves the top cover to the top of the culture dish, the clamp 17 is driven to the position of the culture dish by the vertical moving slide 22, and the culture dish is re-fixed. The electric suction cup 13 resets the cover body, so that the clamp 17 drives the culture dish to move to the top of the stacking mechanism 7 again.
Claims
1. A clone picking module, comprising a frame (1) and an industrial camera (2), characterized in that: A top plate (6) is mounted on the top of the frame (1) by bolts, and a fixing hole (501) is provided at the top of the top plate (6), and a limit baffle (5) is mounted below the fixing hole (501) by bolts, and an industrial lens (4) is arranged at the center of the limit baffle (5) and the top plate (6), and a connecting member (3) is installed above the industrial lens (4), and the industrial camera (2) is arranged on a side of the connecting member (3) away from the industrial camera (2), a layer plate (8) is provided below the top plate (6), and a rotary shell removal mechanism (10) is provided above the layer plate (8), and a stacking mechanism (7) is provided on the left side of the rotary shell removal mechanism (10), and a horizontal pick-up and placement mechanism (23) is provided on the side of the industrial lens (4) away from the rotary shell removal mechanism (10).
2. The clone selection module according to claim 1, characterized in that: The stacking mechanism (7) comprises a driving motor (701), a connecting base (702), a connecting column (703) and a placement bracket (704), wherein the driving motor (701) drives the connecting base (702) installed above, and the connecting column (703) is installed above the connecting base (702), and the placement bracket (704) is installed on the outer surface of the connecting column (703).
3. The clone selection module according to claim 1, wherein: The rotary shell-taking mechanism (10) comprises a support base (11), a horizontal movable slide (12), an electric suction cup (13), a mounting bracket (14) and a docking plate (1401), wherein the horizontal movable slide (12) is mounted above the support base (11), the electric suction cup (13) is arranged above the horizontal movable slide (12), the left side of the electric suction cup (13) is connected to the docking plate (1401), and the mounting bracket (14) is arranged on the side of the mounting bracket (14) away from the docking plate (1401).
4. The clone selection module according to claim 1, wherein: The horizontal pick-and-place mechanism (23) includes a base plate (16), a clamping jaw (17), a connecting bracket (18), a slider (19), a first servo motor (21) and a second servo motor (24), and two groups of clamping jaws (17) are placed above the base plate (16), and a connecting bracket (18) is installed above the two groups of clamping jaws (17), and a slider (19) is provided on the side of the connecting bracket (18) away from the rotating shell taking component, and a vertical moving slide (22) is provided on the side of the slider (19) away from the connecting bracket (18), and a first servo motor (21) is installed below the base plate (16), and a second servo motor (24) is provided below the vertical moving slide (22).
5. The clone selection module according to claim 1, characterized in that: A placement platform (15) is placed at the center of the rotary shell-taking mechanism (10) and the horizontal placement mechanism (23), and a visual light source (20) is installed below the placement platform (15) via bolts.
6. The clone selection module according to claim 1, characterized in that: The bottom of the layer plate (8) is mounted on the fixed base (9) via bolts, and support blocks (901) are provided below the four groups of fixed bases (9).
Citation Information
Patent Citations
Bacterium picking device for microbial experiments
CN212610593U