Cell traction force loading device

Abstract

The utility model discloses a cell traction force loading device, which is used for realizing multi-mode loading of traction force of living cells in the horizontal direction, can be matched with a high-power microscope to carry out high-resolution real-time dynamic observation on the cells and organelles in a stress application state, and provides technical support for regulating and controlling behaviors of the cells and the organelles by analytic force. The device mainly comprises a frame part, a sliding part and a clamping part, the frame part is of a hollow frame structure formed by fixing a bottom plate, a cover plate, a front guide rod fixing block and a rear guide rod fixing block through bolts, a laser distance measuring sensor is fixedly arranged on the front side of the front guide rod fixing block, and a motor is fixedly arranged on the rear side of the rear guide rod fixing block; the sliding part comprises two guide rods and a sliding block, the sliding block can slide back and forth on the two guide rods, and a tension sensor is fixedly arranged on the rear side of the sliding block; the clamping part is used for clamping a biological membrane. The device can be flattened and is small in size, the cell stretching process is more stable, and the whole device does not need to be soaked in cell sap.

Description

Technical Field

[0001] The utility model relates to the field of cell tension loading devices, in particular to a tension loading device which can cooperate with a microscope to perform high-resolution observation of the changing process of cells and their internal organelles. Background Art

[0002] Distraction osteogenesis is based on the biological properties of bone tissue—its ability to stimulate new bone formation under slow, sustained stretching. However, the processes that influence tissue cells under this stretching force remain largely unknown. Limited by the inability to directly observe in vivo, current research primarily relies on in vitro devices to apply force to cells and observe these changes. Specific cell-applying devices include the following:

[0003] (1) Mainly by controlling the vacuum pump (vacuum pump and storage-variable air pressure system) connected to the vacuum chamber, the negative pressure in the vacuum chamber is periodically changed, thereby deforming the elastic basement membrane of the culture plate sealed thereon, thereby subjecting the cells growing on the wall to mechanical stretching. However, this force-adding method subjects the cells to forces in multiple directions, which is different from the horizontal force generated by stretching. At the same time, due to the connection to the vacuum chamber, real-time high-resolution observation of the cell changes during the force loading process cannot be achieved;

[0004] (2) Using polydimethylsiloxane membrane (PDMS) and a high-precision stepper motor to achieve cell tensile loading. This force loading mode is similar to tensile loading, but the stretching device and PDMS membrane are integrated into the host. Cells can only be observed at low magnification (~10×) before and after stretching, and high-resolution dynamic monitoring of cell changes under force is not possible.

[0005] (3) Uniaxial forces similar to stretching can be applied, but this requires the use of a sterile disposable silicone plate made of a thin transparent film, which is thicker than 0.17 mm and cannot achieve high-resolution observation with an oil immersion lens of more than 60 times;

[0006] (4) It integrates a cell mechanical loading instrument test system and an environmental control system, which can culture bones, cartilage, or any other disc-shaped samples under force, but cannot achieve force-added culture of cells;

[0007] (5) Cell stretch culture system, which can provide periodic stretching force to cells, but because it integrates the cell culture chamber and the force-applying device in a closed space, it is impossible to achieve real-time observation of the cell force-applying process.

[0008] It can be seen that the existing devices have obvious deficiencies in the force application mode and real-time high-resolution observation of cell changes under the force application state. There is an urgent need for a distraction force loading device that is consistent with the force application method of distraction osteogenesis and can be used in conjunction with a high-power microscope to perform high-resolution observation of the changes in cells and their internal organelles. Utility Model Content

[0009] The main purpose of the utility model is to provide a cell tension loading device, so as to enable a high-power microscope to be used for high-resolution observation of the changing process of the internal organelles of cells under a tension state.

[0010] To achieve the above-mentioned purpose, the utility model provides a cell tension loading device, which mainly includes a frame part, a sliding part and a clamping part; the frame part is a hollow frame structure formed by fixing a bottom plate, a cover plate, a front guide rod fixing block and a rear guide rod fixing block by bolts, the front guide rod fixing block has a hole in the middle, and a laser ranging sensor is fixed on its front side, the rear guide rod fixing block has a hole in the middle, and a motor is fixed on its rear side; the sliding part includes two guide rods and a slider, the two ends of the guide rods are respectively fixed on the front guide rod fixing block and the rear guide rod fixing block, the slider can slide back and forth on the two guide rods, and a tension sensor is fixed on the rear side of the slider, and the rear end of the tension sensor is connected to the motor telescopic shaft of the motor; the clamping part is used to clamp the biofilm, the front end of which is fixed to the lower side of the front part of the bottom plate, and the rear end is fixed to the lower side of the slider.

[0011] Optionally, the front and rear ends of the clamping part are composed of a membrane limiting block and a ball head positioning pin, wherein the front end membrane limiting block is fixed on the base plate, and the rear end membrane limiting block is fixed on the slider. The biofilm adopts market standard parts and can be fixed directly using the ball head positioning pin.

[0012] Optionally, comb-shaped baffles are provided on both sides of the middle of the clamping portion to prevent leakage of the culture solution.

[0013] Optionally, the surface of the slider is sandblasted to increase its surface roughness, thereby ensuring that the laser emitted by the laser ranging sensor is diffusely reflected after hitting the slider surface, and the reflected light can enter the receiving window of the laser ranging sensor to ensure accurate distance measurement.

[0014] Optionally, the slider and the guide rod are connected to the linear bearing via a retaining spring so as to firmly connect the slider and the linear bearing.

[0015] Optionally, the motor is a servo motor, which can control the stretching distance more accurately.

[0016] Optionally, the motor and the rear guide rod fixing block are connected via a motor fixing block, and the tension sensor and the motor telescopic shaft are connected via a motor transition piece.

[0017] Optionally, the frame portion, the sliding portion and the clamping portion are all made of anti-corrosion materials.

[0018] The cell tension loading device proposed in the embodiment of the present invention can be used in conjunction with a high-power microscope to perform high-resolution observation of the changes in the internal organelles of cells under a force-applied state. At the same time, the device can be flattened and compact, and the cell stretching process is more stable, without the need to immerse the entire device in cell fluid. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a structural diagram of the cell tension loading device of the utility model;

[0020] Figure 2 This is a structural diagram of the cell tension loading device (back) of the present invention.

[0021] Marking instructions: 1-base plate, 2-cover plate, 3-laser ranging sensor, 4-front guide rod fixing block, 5-rear guide rod fixing block, 6-motor fixing block, 7-motor telescopic shaft, 8-motor, 9-guide rod, 10-slider, 11-linear bearing, 12-tension sensor, 13-motor transition piece, 14-biofilm, 15-front end membrane limit block, 16-rear end membrane limit block, 17-ball head positioning pin, 18-hole one, 19-comb baffle two, 20-comb baffle one, 21-hole two DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0023] The above description is merely a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in the present invention should be included in the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be based on the scope of protection of the claims.

[0024] The cell tension loading device of the present utility model mainly includes a frame part, a sliding part and a clamping part. Figure 1 、 Figure 2As shown, the frame portion is a hollow frame structure formed by fixing a base plate 1, a cover plate 2, a front guide rod fixing block 4 and a rear guide rod fixing block 5 by bolts; a hole 18 is provided in the middle of the front guide rod fixing block 4, and a laser ranging sensor 3 is fixedly provided on the front side of the front guide rod fixing block 4, and the laser ranging sensor 3 transmits and receives lasers through the hole 18, and the hole 18 is preferably square; a hole 21 is provided in the middle of the rear guide rod fixing block 5, and a motor 8 is fixed on the rear side, and the hole 21 is preferably circular, and the motor telescopic shaft 7 of the motor 8 can pass through the hole 21, and the motor 8 is preferably a servo motor, so as to more accurately control the stretching distance, and the motor 8 and the rear guide rod fixing block 5 are preferably connected through the motor fixing block 6.

[0025] The sliding part includes two guide rods 9 and a slider 10, the two ends of the two guide rods 9 are respectively fixed on the front guide rod fixing block 4 and the rear guide rod fixing block 5, and the slider 10 can slide back and forth on the two guide rods; optimally, the slider 10 and the guide rod 9 are connected to the linear bearing 11 through a retaining spring so as to firmly connect the slider 10 and the linear bearing 11; at the same time, the surface of the slider 10 is optimally sandblasted to increase its surface roughness, thereby ensuring that the laser emitted by the laser ranging sensor 3 is diffusely reflected after being irradiated on the surface of the slider 10, and the reflected light can enter the receiving window of the laser ranging sensor, so that the distance can be measured more accurately; a tension sensor 12 is fixedly arranged on the rear side of the slider 10, and the rear end of the tension sensor 12 is connected to the motor telescopic shaft 7 of the motor 8. Optimally, the tension sensor 12 is connected to the motor telescopic shaft through a motor transition piece 13.

[0026] The clamping part is used to clamp the biofilm 14, and the front end of the clamping part is fixed to the lower side of the front part of the base plate 1, and the rear end is fixed to the lower side of the slider 10; optimally, the front and rear ends of the clamping part are composed of membrane limiting blocks 15, 16 and ball head positioning pins 17, wherein the front end membrane limiting block 15 is fixed on the base plate 1, and the rear end membrane limiting block 16 is fixed on the slider 10, and the biofilm 14 adopts market standard parts, and the biofilm 14 can be fixed directly using the ball head positioning pins 17; in addition, it is best to provide comb-shaped baffle 2 19 and comb-shaped baffle 1 20 on both sides in the middle of the clamping part, wherein the comb-shaped baffle 1 20 is connected to the front end membrane limiting block 15, and the comb-shaped baffle 2 19 is connected to the rear end membrane limiting block 16, and the comb-shaped baffle 2 19 can slide back and forth with the slider 10. When the slider 10 slides to the maximum position, the comb-shaped baffle 2 19 and the comb baffle 1 20 can also jointly prevent the leakage of culture fluid.

[0027] The frame portion, the sliding portion and the clamping portion are preferably made of corrosion-resistant materials.

[0028] When in use, the device of the present invention can be placed on a custom-made tabletop to ensure the free sliding of the slider 10. When the motor 8 rotates, it drives the slider 10 to move axially about the guide rod 9. The dual guide rod structure ensures that the biofilm 14 does not deform. At the same time, the laser rangefinder 3 and the tension sensor 12 can detect the stretching amount and stretching force of the biofilm 14 at any time. In addition, a high-power microscope can be placed under the biofilm 14 to clearly observe the entire process of cell changes under the stretching state.

[0029] The cell tension loading device proposed in the embodiment of the present invention can be used in conjunction with a high-power microscope to perform high-resolution observation of the changes in the internal organelles of cells under a force-applied state. At the same time, the device can be flattened and compact. Due to the use of a double-slide structure, the cell stretching process is more stable, and when using a matching high-power microscope for observation, the entire device does not need to be immersed in cell fluid.

Claims

1. A cell tension loading device, characterized in that: The device mainly comprises a frame part, a sliding part and a clamping part; the frame part is a hollow frame structure formed by fixing a bottom plate (1), a cover plate (2), a front guide rod fixing block (4) and a rear guide rod fixing block (5) by bolts; the front guide rod fixing block (4) has a hole (18) in the middle, and a laser distance sensor (3) is fixedly arranged on the front side; the rear guide rod fixing block (5) has a hole (21) in the middle, and a motor (8) is fixedly arranged on the rear side; the sliding part comprises two guide rods (9) and a The slider (10) has two ends of the guide rod (9) fixed on a front guide rod fixing block (4) and a rear guide rod fixing block (5), respectively. The slider (10) can slide back and forth on the two guide rods (9). A tension sensor (12) is fixedly provided on the rear side of the slider (10). The rear end of the tension sensor (12) is connected to the motor telescopic shaft (7) of the motor (8); the clamping portion is used to clamp the biofilm (14), the front end of which is fixed on the lower side of the front part of the base plate (1), and the rear end is fixed on the lower side of the slider (10).

2. The cell tension loading device according to claim 1, characterized in that: The front and rear ends of the clamping portion are both composed of film limiting blocks and ball head positioning pins (17), wherein the front film limiting block (15) is fixed on the bottom plate (1), and the rear film limiting block (16) is fixed on the slider (10).

3. The cell tension loading device according to claim 2, characterized in that: Comb-shaped baffles are provided on both sides of the middle of the clamping portion, wherein the first comb-shaped baffle (20) is connected to the front end film limiting block (15), and the second comb-shaped baffle (19) is connected to the rear end film limiting block (16).

4. The cell tension loading device according to claim 1, characterized in that: The surface of the slider (10) is sandblasted.

5. The cell tension loading device according to claim 1, characterized in that: The slider (10) and the guide rod (9) are connected to the linear bearing (11) via a clamping spring.

6. The cell tension loading device according to claim 1, characterized in that: The motor (8) is a servo motor.

7. The cell tension loading device according to claim 1, characterized in that: The motor (8) and the rear guide rod fixing block (5) are connected via a motor fixing block (6).

8. The cell tension loading device according to claim 1, characterized in that: The tension sensor (12) is connected to the motor telescopic shaft (7) via a motor transition piece (13).

9. The cell tension loading device according to claim 3, characterized in that: The frame part, the sliding part and the clamping part are all made of anti-corrosion materials.

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