Magnetic bead suspension device

Abstract

The utility model relates to a magnetic bead suspension device, and belongs to the technical field of experimental instruments and equipment. The magnetic bead suspension device comprises a shell, a reaction container, a containing disc and an electrostatic generation device, and the magnetic bead suspension device generates electrostatic field force through sliding friction on the contact surfaces of a first friction piece and a second friction piece, so that magnetic beads in the reaction container are suspended in a solution in the reaction container. The magnetic beads can be stably suspended in the container in a mode of generating electrostatic field force through friction, and compared with a traditional vibration, stirring and uniform mixing mode, the conditions of magnetic bead sedimentation and wall collision can be avoided, so that the magnetic beads can be in full contact with a coating object or a conjugate, and the final effector concentration is enhanced.

Description

Technical Field

[0001] The present application relates to the technical field of experimental instruments and equipment, and in particular to a magnetic bead suspension device. Background Art

[0002] With the completion of the Human Genome Project and the continuous development of sequencing technology, magnetic separation technology represented by biological magnetic beads has been widely used in the fields of cell separation, protein purification, immunology and microbiology detection. Nano-scale magnetic beads are often used, and a functional group is marked on the surface of the magnetic beads, which can undergo adsorption reaction with biological macromolecules such as nucleic acids or proteins.

[0003] Traditional magnetic bead coating or adsorption processes are often carried out in a container, using an external oscillator or stirring component to achieve the binding and mixing of biological sample molecules and magnetic beads.

[0004] In the prior art, Chinese Patent Publication No. CN212855712U discloses a preparation device for preparing a magnetic bead mother solution. The preparation device includes a reaction container, a mixing device, a temperature control device, and a magnetic separation device. The reaction container is used to contain at least two substances, one of which includes magnetic beads. The mixing device is arranged inside or outside the reaction container, the temperature control device is arranged inside or outside the reaction container, and the magnetic separation device is arranged inside or outside the reaction container. The mixing device is used to mix the at least two substances contained in the reaction container. The mixing device includes a mixing assembly, which includes: a stirring member extending into the reaction container, the stirring member including a stirring rod and a blade, the blade being arranged on the stirring rod, the free ends of the stirring rod and the blade being provided with openings, and the stirring rod and the blade being connected to each other; and a stirring drive member, the stirring drive member driving the stirring member to rotate so that the at least two substances in the reaction container are evenly mixed.

[0005] In addition to the stirring method mentioned above, vibration is also commonly used. The mixing of magnetic beads and solution is achieved by vibration or stirring. However, magnetic beads have a certain gravity. If the vibration amplitude is not enough, the magnetic beads will often precipitate locally or aggregate into clumps. Mixing is difficult. In addition, the vibration mixing method may cause loss of beads due to adhesion to the cover / wall during the coating and incubation process. All of the above situations may result in low binding between magnetic beads and samples, resulting in low final effector concentration, thereby affecting the subsequent application of the effector.

[0006] Therefore, the present application proposes a magnetic bead suspension device that generates electrostatic field force through friction to enable the magnetic beads to be stably suspended in a container. Compared with traditional vibration and stirring mixing methods, it can avoid the sedimentation and collision of magnetic beads, allowing the magnetic beads to fully contact the coating or binding substance, thereby increasing the concentration of the final effector. Utility Model Content

[0007] In order to overcome the problems existing in the related art, the purpose of this application is to provide a magnetic bead suspension device, including a shell, a reaction container, a holding plate and an electrostatic generating device, which generates an electrostatic field force by sliding friction on the contact surface of a first friction member and a second friction member, so that the magnetic beads in the reaction container are suspended in the solution of the reaction container, which can prevent the magnetic beads from settling and hitting the wall, and enable the magnetic beads to fully contact with the coating or binding substance, thereby increasing the concentration of the effector.

[0008] A magnetic bead suspension device comprises a housing, a reaction container, a holding tray and an electrostatic generator, wherein the reaction container, the holding tray and the electrostatic generator are all arranged in the housing;

[0009] The static electricity generating device includes a first friction member and a second friction member arranged in a vertical direction, wherein friction materials are provided on contact surfaces of the first friction member and the second friction member, and the friction materials of the first friction member and the second friction member have different friction electrode sequences;

[0010] The electrostatic generating device further includes a driving member and a supporting member;

[0011] The first friction member is insulated and attached to the driving member, and the second friction member is insulated and attached to the supporting member.

[0012] The first friction member can rotate relative to the second friction member, thereby sliding and rubbing on the contact surface of the first friction member and the second friction member to generate an electrostatic field force, so that the magnetic beads in the reaction container are suspended in the solution of the reaction container.

[0013] In a preferred technical solution of the present application, the magnetic bead suspension device further includes a heating device, which includes a temperature controller and a heating element, and the heating device is disposed in the housing.

[0014] In a preferred technical solution of the present application, the driving member is a ceiling fan, and the first friction member is attached to the blades of the ceiling fan.

[0015] In a preferred technical solution of the present application, the friction material of the first friction member or the second friction member is any one of aniline formaldehyde resin, polyoxymethylene, ethyl cellulose, polyamide nylon 11, polyamide nylon 66, wool and its fabrics, silk and its fabrics, paper, polyethylene glycol succinate, cellulose, cellulose acetate, polyethylene glycol adipate, polydiallyl phthalate, regenerated cellulose sponge, cotton and its fabrics, polyurethane elastomer, styrene-acrylonitrile copolymer, styrene-butadiene copolymer, wood, hard rubber, acetate, rayon, polymethyl methacrylate, polyvinyl alcohol, polyester, copper, aluminum, gold, silver and steel, and the friction material of the first friction member or the second friction member is any one of polystyrene, polyethylene, polypropylene, polydiphenylpropane carbonate, polyethylene terephthalate, polyimide, polyvinyl chloride, polydimethylsiloxane, polychlorotrifluoroethylene and polytetrafluoroethylene.

[0016] In a preferred technical solution of the present application, the magnetic bead suspension device further includes a motor assembly, the holding tray is connected to the motor assembly so that the holding tray can rotate, and a receiving groove is provided on the holding tray.

[0017] In a preferred technical solution of the present application, the reaction container includes a centrifuge tube or a reagent bottle.

[0018] In a preferred technical solution of the present application, when the particle size of the magnetic beads is 1.5 um, the rotation speed of the driving member is greater than 500 rpm.

[0019] In a preferred technical solution of the present application, the second friction member is attached to the support member in a circumferential manner, and the second friction member is in the shape of an inverted triangular prism.

[0020] In a preferred technical solution of the present application, the holding tray is installed at an angle in the shell, and the angle between the placement surface of the holding tray and the horizontal plane is in the range of 20°-40°.

[0021] In a preferred technical solution of the present application, a guide rail is further provided on the inner wall of the shell, and the electrostatic generating device is mounted on the guide rail via a mounting member. The distance between the electrostatic generating device and the reaction container can be adjusted along the length direction of the guide rail.

[0022] The beneficial effects of the technical solution provided by the present application are as follows: the magnetic bead suspension device of the present application includes a shell, a reaction container, a holding plate and an electrostatic generating device, which generates an electrostatic field force opposite to gravity through sliding friction on the contact surface of the first friction part and the second friction part, so that the magnetic beads in the reaction container are suspended in the solution of the reaction container.

[0023] (1) The electrostatic field force generated by friction enables the magnetic beads to be stably suspended in the container. Compared with the traditional vibration mixing method, it can avoid the sedimentation and collision of the magnetic beads, allowing the magnetic beads to fully contact with the coating or binding substance, thereby increasing the concentration of the effector.

[0024] (2) The current method of keeping the magnetic beads suspended and flowing by a rotating disk or a vibrating disk, but without temperature control equipment, causes the biological molecules to bind slowly at room temperature. The present suspension device not only makes the suspension state more thorough through the electrostatic field force, thus reducing the situation where the magnetic beads coating adheres to the wall, but also ensures the reaction temperature, further accelerating the binding speed of the biological molecules and improving the efficiency of the experimental operation.

[0025] (3) The magnetic bead suspension device provides a method for placing a large number of samples by setting a holding plate and a reaction container, so that the magnetic beads are suspended in the liquid and the test object is in a flowing state. This allows for large-scale magnetic bead coating, incubation, and activation, while also increasing the concentration of the final effector. For example, conventional immunoassay methods can detect positive results, reducing false negatives and improving detection sensitivity.

[0026] (4) The magnetic bead suspension device can sustainably perform operations such as magnetic bead activation, coating, and incubation of biological molecules (such as antibodies and antigen molecules). The magnetic beads are always in a suspended state in the liquid, so that the surface of the magnetic beads is completely exposed to the solution, providing a more sufficient contact area, thereby improving the efficiency of magnetic bead activation, coating, and incubation. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is the magnetic bead suspension device shown in the embodiment of the present application;

[0028] Figure 2 It is the serving tray shown in the embodiment of the present application;

[0029] Figure 3 Schematic diagram of the force on the magnetic beads shown in the embodiment of the present application;

[0030] Figure 4 This is a schematic diagram of the second friction member shown in the embodiment of the present application. Figure 1 ;

[0031] Figure 5 This is a schematic diagram of the second friction member shown in the embodiment of the present application. Figure 2 ;

[0032] Figure 6 This is the experimental verification result diagram of this application.

[0033] Reference numerals:

[0034] 1. Shell; 2. Reaction container; 3. Serving tray; 31. Receiving tank; 4. Electrostatic generating device; 41. First friction member; 42. Second friction member; 43. Driving member; 44. Support member; 5. Heating device; 6. Motor assembly; 7. Magnetic beads. DETAILED DESCRIPTION

[0035] The following describes preferred embodiments of the present invention in more detail with reference to the accompanying drawings. Although preferred embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. Rather, these embodiments are provided to make the present invention more thorough and complete, and to fully convey the scope of the present invention to those skilled in the art.

[0036] The terms used in this utility model are for the purpose of describing specific embodiments only and are not intended to limit the utility model. As used in this utility model and the appended claims, the singular forms "a," "the," and "the" are intended to include the plural forms, unless the context clearly indicates otherwise. It should also be understood that the term "and / or" as used herein refers to and includes any and all possible combinations of one or more of the associated listed items.

[0037] It should be understood that although the terms "first", "second", "third", etc. may be used in the present invention to describe various information, such information should not be limited to these terms. These terms are only used to distinguish information of the same type from each other. For example, without departing from the scope of the present invention, the first information may also be referred to as the second information, and similarly, the second information may also be referred to as the first information. Thus, features defined as "first" or "second" may explicitly or implicitly include one or more of such features. In the description of the present invention, "multiple" means two or more, unless otherwise clearly and specifically defined.

[0038] Example 1

[0039] Existing magnetic bead coating and incubation devices mostly use shaking or stirring methods. This method does not mix the magnetic beads and solution sufficiently, resulting in a low final effector concentration. It also requires a considerable amount of time to fully mix the solution, which can also result in low final effector concentrations, affecting detection sensitivity and failing to meet the needs of large-scale testing and analysis. Therefore, it is necessary to improve traditional magnetic bead coating and incubation devices to ensure that the magnetic beads are in full contact with the coating or binding agent, thereby increasing the concentration of the effector.

[0040] Magnetic bead coating is the process of fixing specific molecules (referred to as coatings in this application), such as proteins, nucleic acids or small molecule ligands, on the surface of magnetic beads to facilitate subsequent experimental operations, such as immunoassays, nucleic acid extraction, cell separation, affinity purification, etc.

[0041] Some types of magnetic beads require activation before use, such as using chemical reagents such as electrolytic diimide (EDC) and N-hydroxysuccinimide (NHS) to activate carboxyl magnetic beads.

[0042] Through magnetic bead activation, chemical groups that can covalently bind to target molecules are formed on the surface of the magnetic beads.

[0043] Magnetic bead incubation involves mixing magnetic beads with target molecules and maintaining them under specific conditions for a period of time to promote binding between the two.

[0044] The effector in this application refers to the product after being fixed on the surface of magnetic beads, such as proteins, nucleic acids or small molecule ligands; the product of magnetic beads activating the surface of magnetic beads to form chemical groups that can covalently bind to target molecules; and the binding product after the magnetic beads and target molecules are mixed and maintained under specific conditions for a period of time.

[0045] Figure 1 This application embodiment shows Figure 1 ;

[0046] See also Figure 1 .

[0047] The magnetic bead suspension device of the embodiment of the present application includes a housing 1, a reaction container 2, a holding tray 3 and an electrostatic generator 4, wherein the reaction container 2, the holding tray 3 and the electrostatic generator 4 are all arranged in the housing 1;

[0048] The reaction container 2 is placed on the holding tray 3; the reaction container 2 of the embodiment of the present application is used to hold the magnetic beads 7 and other reaction reagents, and provides a place for the magnetic beads 7 to be coated, incubated, and activated.

[0049] The electrostatic generating device 4 is located above or below the reaction container 2 ; further, the magnetic bead suspension device of the embodiment of the present application is exemplarily arranged such that the electrostatic generating device 4 is located above the reaction container 2 .

[0050] The static electricity generating device 4 of the embodiment of the present application includes a first friction member 41 and a second friction member 42 arranged in a vertical direction. Friction materials are provided on the contact surfaces of the first friction member 41 and the second friction member 42. The friction materials of the first friction member 41 and the second friction member 42 have different friction electrode sequences.

[0051] The electrostatic generating device further includes a driving member 43 and a supporting member 44;

[0052] The first friction member 41 is insulated and attached to the driving member 43, and the second friction member 42 is insulated and attached to the supporting member 44.

[0053] The first friction member 41 can rotate relative to the second friction member 42 , thereby sliding and rubbing on the contact surface of the first friction member 41 and the second friction member 42 to generate an electrostatic field force, so that the magnetic beads 7 in the reaction container 2 are suspended in the solution of the reaction container 2 .

[0054] The magnetic bead suspension device also includes a heating device 5, which includes a temperature controller and a heating element. The heating device 5 is disposed within the housing 1. For example, the heating element in the embodiment of the present application is a PTC thermistor. The heating device 5 in the embodiment of the present application can set the desired operating temperature of the magnetic bead suspension device.

[0055] Exemplarily, the electrostatic generating device 4 is located above the reaction container 2, and the first friction part 41 is located above the second friction part 42. The friction material of the second friction part 42 of the magnetic bead suspension device of the embodiment of the present application can be made of rubber or other materials with strong electrical power. Exemplarily, the friction material used for the second friction part 42 of the present embodiment is rubber, and the friction material of the first friction part 41 is cashmere with strong electron-loss ability or other materials with strong electron-loss ability. Exemplarily, the friction material of the first friction part 41 in the embodiment of the present application is cashmere material. The first friction part 41 and the second friction part 42 rotate in opposite directions or the first friction part 41 rotates and the second friction part 42 does not rotate. The second friction part 42 obtains electrons by friction during differential rotation to generate an electrostatic field force. Since the second friction part 42 is closer to the reaction container 2, a stronger electrostatic field force is generated for the magnetic beads 7. The first friction member 41 rotates in the opposite direction to the second friction member 42 during differential rotation, and generates an electrostatic field force by losing electrons through friction. Since the first friction member 41 is farther away from the reaction container 2, the electrostatic field force generated is smaller than that generated by the second friction member 42. The difference in electrostatic field force is the actual electrostatic field force exerted on the magnetic beads 7, which is opposite to the force of gravity.

[0056] The magnetic bead suspension device of the embodiment of the present application can achieve stable suspension of the magnetic beads 7 in the solution of the reaction container 2 by adjusting the material selection, amount of material and distance between the electrostatic generating device 4 and the reaction container 2 of the first friction member 41 and the second friction member 42 according to the size of the magnetic beads 7 in the reaction container 2.

[0057] Furthermore, the magnetic bead suspension device of the embodiment of the present application is also provided with a heating device 5, which includes a temperature controller and a heating element. The temperature controller can control the heating element to provide a stable heating temperature for the magnetic bead suspension device. Because at room temperature, the reaction of most biological molecules is relatively slow, the speed at which the magnetic beads 7 are coated or bound to adsorb biological molecules is extremely slow, resulting in a long operation time. The present magnetic bead suspension device can provide temperature-controllable heating conditions and provide the optimal reaction temperature for the coating of the magnetic beads 7, thereby improving the efficiency of the coating of the magnetic beads 7. Furthermore, the holding tray 3 of the embodiment of the present application is set to be tilted at a certain angle, providing the magnetic beads 7 with a force condition that is relatively static relative to the direction of gravity, such as Figure 3 shown.

[0058] Beneficial effects of the embodiments of the present application: The magnetic bead suspension device of the present application includes a shell, a reaction container, a holding plate and an electrostatic generating device, which generates an electrostatic field force by sliding friction on the contact surface of the first friction part and the second friction part, so that the magnetic beads in the reaction container are suspended in the solution of the reaction container.

[0059] (1) The electrostatic field force generated by friction enables the magnetic beads to be stably suspended in the container. Compared with the traditional vibration and stirring mixing method, it can avoid the sedimentation and collision of the magnetic beads, so that the magnetic beads can fully contact with the coating or binding substance, thereby increasing the concentration of the target product.

[0060] (2) The current method of keeping the magnetic beads suspended and flowing by a rotating disk or a vibrating disk, but without temperature control equipment, causes the biological molecules to bind slowly at room temperature. The present suspension device not only makes the suspension state more thorough through the electrostatic field force, thus reducing the situation where the magnetic beads coating adheres to the wall, but also ensures the reaction temperature, further accelerating the binding speed of the biological molecules and improving the efficiency of the experimental operation.

[0061] Example 2

[0062] The embodiments of this application will further invent and design the magnetic bead suspension device.

[0063] In addition to the structural features of the above-mentioned embodiments, the magnetic bead suspension device of the embodiment of the present application does not limit the specific structure of the driving member 43. For example, the driving member 43 of the embodiment of the present application is a ceiling fan, and the first friction member 41 is provided on the blades of the ceiling fan.

[0064] Furthermore, the friction material of the first friction member 41 or the second friction member 42 is any one of aniline formaldehyde resin, polyoxymethylene, ethyl cellulose, polyamide nylon 11, polyamide nylon 66, wool and its fabric, silk and its fabric, paper, polyethylene glycol succinate, cellulose, cellulose acetate, polyethylene glycol adipate, polydiallyl phthalate, regenerated cellulose sponge, cotton and its fabric, polyurethane elastomer, styrene-acrylonitrile copolymer, styrene-butadiene copolymer, wood, hard rubber, acetate, rayon, polymethyl methacrylate, polyvinyl alcohol, polyester, copper, aluminum, gold, silver and steel, and the friction material of the first friction member 41 or the second friction member 42 is any one of polystyrene, polyethylene, polypropylene, polydiphenylpropane carbonate, polyethylene terephthalate, polyimide, polyvinyl chloride, polydimethylsiloxane, polychlorotrifluoroethylene and polytetrafluoroethylene.

[0065] For example, when the friction material of the first friction member 41 is wool or wool fabric, the friction material of the second friction member 42 may be made of polypropylene.

[0066] Furthermore, the magnetic bead suspension device includes a motor assembly 6, to which the holding tray 3 is connected for rotation. The holding tray 3 is provided with a receiving groove 31. The reaction vessel 2 comprises a centrifuge tube or a reagent bottle. The rotatable holding tray 3 further promotes the flow of the solution in the reaction vessel 2, allowing for more thorough mixing of the magnetic beads 7 with the solution, thereby improving coating efficiency.

[0067] Furthermore, the ceiling fan provided with the first friction member 41 in the embodiment of the present application is hung on the housing 1 .

[0068] The support member 44 of the embodiment of the present application may further include a turntable and a motor, the second friction member 42 is adhered to the turntable, and further includes a motor shaft, the rotation of the motor drives the motor shaft to rotate, the turntable is mounted on the motor shaft, and the motor shaft drives the turntable to rotate.

[0069] like Figure 2 As shown, the holding tray 3 is installed in an inclined manner in the housing 1, and the angle between the holding tray 3 and the horizontal plane is in the range of 20°-40°.

[0070] The specific angle can be set according to the actual application, such as Figure 3 As shown, the embodiment of the present application is exemplarily set to 30°. The setting of the angle can enable the centrifugal force F1 generated by the reaction container 2 during rotation to be decomposed into a component force F2 in the vertical direction, thereby achieving a state of equilibrium with the electrostatic field force generated by the electrostatic generating device 4, so that the magnetic beads 7 can be stably suspended in the solution.

[0071] Furthermore, a guide rail is provided on the inner wall of the shell 1 of the magnetic bead suspension device of the embodiment of the present application, and the electrostatic generating device 4 is installed on the guide rail through a mounting part. For example, the mounting part can be a mounting rod and a nut, so that the electrostatic generating device 4 can adjust the distance from the reaction container 2 along the length direction of the guide rail.

[0072] The beneficial effects of the embodiments of the present application are as follows: by sliding friction on the contact surface of the first friction part and the second friction part to generate an electrostatic field force, the magnetic beads in the reaction container are suspended in the solution of the reaction container. By setting up the holding tray and the reaction container, a method is provided for placing a large number of samples, so that the magnetic beads are in a suspended state in the liquid and the test objects are in a flowing state. Large-scale coating and incubation can be achieved, and at the same time, the concentration of the biomarkers being tested can be increased, so that conventional immunoassay methods can be used to detect positive results, reduce false negatives, and improve detection sensitivity. It can act on a large number of samples, is environmentally friendly, portable, and has good universality, and is suitable for POCT, screening and other application scenarios.

[0073] Example 3

[0074] The magnetic bead suspension device of the embodiment of the present application includes a housing 1, a reaction container 2, a holding tray 3 and an electrostatic generator 4, wherein the reaction container 2, the holding tray 3 and the electrostatic generator 4 are all arranged in the housing 1;

[0075] The static electricity generating device 4 of the embodiment of the present application includes a first friction member 41 and a second friction member 42 arranged in a vertical direction, and the first friction member 41 is disposed above the second friction member 42 .

[0076] Friction materials are provided on the contact surfaces of the first friction member 41 and the second friction member 42. The friction material of the first friction member 41 in the embodiment of the present application is cashmere material, and the second friction member 42 is composed of a rubber block. The first friction member 41 is insulated and attached to the driving member 43, and the second friction member 42 is insulated and attached to the supporting member 44.

[0077] For example, the rubber blocks of the second friction member 42 of the embodiment of the present application are circumferentially distributed on the support member 44, such as Figure 4 Furthermore, the rubber block is in the shape of an inverted triangular prism. Compared with a rectangular parallelepiped rubber block, the volume of the inverted triangular prism rubber block is only one-third of that of the rectangular parallelepiped rubber block when the friction surface is the same size. The reduced volume reduces the difficulty of electron movement in the second friction member 42, thereby increasing the electrostatic field force.

[0078] For example, the first friction member 41 of the present embodiment is insulated and attached to a ceiling fan. In actual use, the rotational speed of the first friction member 41 is greater than or equal to 500 rpm. In practice, the rotational speed of the first friction member 41 of the magnetic bead suspension device is selected based on the size of the magnetic beads, the distance between the electrostatic generator 4 and the reactor 2, and the friction material of the electrostatic generator 4. The larger the particle size of the magnetic beads 7, the greater the rotational speed.

[0079] For example, when the particle size of the magnetic beads is 1.5um and the rotation speed of the driving member 43 is 500rpm, when the rotation speed of the driving member 43 is balanced, a constant electrostatic force field can be formed instantly. For example, in the magnetic bead suspension device of the present application, since the first friction member 41 is insulated and attached to the driving member 43, and the second friction member 42 is insulated and attached to the support member 44, when the driving member 43 stops rotating, the electrostatic field force can still be maintained for more than 15 minutes. The maintenance of the electrostatic field force can ensure that during the experimental operation, although the previous operation such as (incubation, activation, resuspension, etc.) has been completed and stopped rotating, the next operation cannot be performed in time due to other reasons, such as when the machine cannot be tested, the magnetic bead suspension device can still ensure that the magnetic beads 7 do not settle, thereby ensuring the sensitivity of the detection.

[0080] Furthermore, a heating device 5 can be provided at the bottom of the magnetic bead suspension device. The heating device 5 includes a thermostat and a heater, and is disposed within the housing 1. For example, the heater in the embodiment of the present application utilizes a PTC thermistor. The heating device 5 in the embodiment of the present application can set the desired operating temperature of the magnetic bead suspension device. For example, 37°C is the optimal temperature range for antibody-antigen reactions.

[0081] The reaction container 2 of the embodiment of the present application adopts a centrifuge tube. Furthermore, the holding plate 3 can adopt a centrifuge turntable, which is provided with a placement groove. The magnetic beads and the reaction solution are added to the centrifuge tube, and the centrifuge tube is placed on the placement groove. The centrifuge turntable can make the material in the centrifuge tube flow, and the electrostatic field force generated by the electrostatic generating device 4 makes the magnetic beads 7 stably suspended in the solution.

[0082] Experimental Example 1

[0083] The lipoarabinomannan (LAM) antigen was diluted to a concentration of 5 pg / mL using the calibration diluent.

[0084] (1) Take 4 mL of the above LAM antigen sample and add 60 μg of magnetic bead reagent (magnetic beads coated with LAM capture antibody, obtained by covalently coupling the amino group on the LAM capture antibody with the carboxyl group on the magnetic beads);

[0085] (2) Incubate at room temperature for 2 h;

[0086] (3) Use a magnetic separator to separate and discard the supernatant.

[0087] (4) Resuspend the magnetic beads with 200 μl of diluent (50 mM TBS + 0.15 M NaCl + 0.1% Tween 20 + 1% BSA, pH 7.4), vortex and mix thoroughly.

[0088] Experimental group 1 (two replicates) was suspended in the magnetic bead suspension device for 0, 2, 4, 6, 8, and 10 minutes before being tested on the machine;

[0089] The control group 1 (two replicates) was tested on the machine after standing for 0, 2, 4, 6, 8, and 10 minutes.

[0090] The test results are as follows:

[0091] Table 1

[0092]

[0093] According to the data in Table 1 and Figure 6 It can be seen that by using the magnetic bead suspension device of the present application, the luminescence value of the magnetic beads in the suspended state can be maintained without decreasing and can be slightly increased, and the settled magnetic beads can be promoted to be further suspended, thereby ensuring the sensitivity of the detection system.

[0094] Experimental Example 2

[0095] LAM (Lipoarabinomannan) antigen was serially diluted with calibrator diluent to 0 pg / mL, 10 pg / mL, 100 pg / mL, and 1000 pg / mL;

[0096] (1) Take 4 mL of each gradient LAM antigen sample and add 60 μg of magnetic bead reagent (magnetic beads coated with LAM capture antibodies, obtained by covalently coupling the amino groups on the LAM capture antibodies with the carboxyl groups on the magnetic beads);

[0097] (2) Control group 2 (two replicates) was incubated in an existing incubation device (using a shaking mixing device) at room temperature for 2 h;

[0098] Experimental group 2 (two replicates) was incubated in the magnetic bead suspension device at room temperature for 2 h;

[0099] (3) Use a magnetic separator to separate and discard the supernatant.

[0100] (4) Resuspend the magnetic beads in 0.3 ml of diluent (50 mM TBS + 0.15 M NaCl + 0.1% Tween 20 + 1% BSA, pH 7.4), vortex to mix, and immediately load onto the instrument for detection.

[0101] The test results are shown in Table 1.

[0102] Table 2

[0103]

[0104]

[0105] It can be seen from the data in Table 2 that the sensitivity of the detection system is significantly improved by using the magnetic bead suspension device of the present application for incubation.

[0106] Unless otherwise specifically stated, the relative arrangement of the parts and steps, numerical expressions and numerical values ​​set forth in these embodiments do not limit the scope of the present application. At the same time, it should be understood that, for ease of description, the sizes of the various parts shown in the drawings are not drawn according to actual proportional relationships. The techniques, methods and equipment known to those of ordinary skill in the relevant art may not be discussed in detail, but where appropriate, the techniques, methods and equipment should be considered as part of the authorization specification. In all examples shown and discussed here, any specific values ​​should be interpreted as being merely exemplary, not as limitations. Therefore, other examples of the exemplary embodiments may have different values. It should be noted that similar numbers and letters represent similar items in the following figures, and therefore, once an item is defined in one figure, it does not need to be further discussed in subsequent figures. In the description of this application, it should be understood that the directions or positional relationships indicated by directional words such as "front, back, up, down, left, right", "horizontal, vertical, vertical, horizontal" and "top, bottom" are usually based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description. Unless otherwise specified, these directional words do not indicate or imply that the device or element referred to must have a specific direction or be constructed and operated in a specific direction. Therefore, they cannot be understood as limiting the scope of protection of this application; the directional words "inside and outside" refer to the inside and outside relative to the outline of each component itself.

[0107] For ease of description, spatially relative terms such as "above", "above", "on the upper surface of", "above", etc. may be used herein to describe the spatial positional relationship of a device or feature to other devices or features as shown in the figures. It should be understood that spatially relative terms are intended to include different orientations of the device in use or operation in addition to the orientation described in the figures. For example, if the device in the drawings is inverted, the device described as "above other devices or structures" or "above other devices or structures" will be positioned as "below other devices or structures" or "below other devices or structures". Thus, the exemplary term "above" can include both "above" and "below". The device can also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatially relative descriptions used here are interpreted accordingly.

[0108] In addition, it should be noted that the use of terms such as "first" and "second" to limit components is only for the convenience of distinguishing the corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore cannot be understood as limiting the scope of protection of this application.

[0109] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that the present invention is susceptible to various modifications and variations. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A magnetic bead suspension device, characterized in that: It comprises a housing (1), a reaction container (2), a holding tray (3) and an electrostatic generating device (4), wherein the reaction container (2), the holding tray (3) and the electrostatic generating device (4) are all arranged in the housing (1); The reaction container (2) is placed on a holding tray (3); the electrostatic generating device (4) is located above the reaction container (2); The electrostatic generating device (4) comprises a first friction member (41) and a second friction member (42) arranged in a vertical direction, friction materials are respectively provided on the contact surfaces of the first friction member (41) and the second friction member (42), and the friction materials of the first friction member (41) and the second friction member (42) have different friction electrode sequences; The static electricity generating device further comprises a driving member (43) and a supporting member (44); The first friction member (41) is insulated and attached to the driving member (43), and the second friction member (42) is insulated and attached to the supporting member (44). The first friction member (41) is capable of rotating relative to the second friction member (42), thereby sliding and rubbing on the contact surface between the first friction member (41) and the second friction member (42) to generate an electrostatic field force, so that the magnetic beads (8) in the reaction container (2) are suspended in the solution of the reaction container (2).

2. The magnetic bead suspension device according to claim 1, characterized in that: The magnetic bead suspension device further comprises a heating device (5), wherein the heating device (5) comprises a temperature controller and a heating element, and the heating device (5) is arranged in the housing (1).

3. The magnetic bead suspension device according to claim 1, characterized in that: The driving member (43) is a ceiling fan, and the first friction member (41) is attached to the blades of the ceiling fan.

4. The magnetic bead suspension device according to claim 1, characterized in that: The friction material of the first friction member (41) or the second friction member (42) is any one of aniline formaldehyde resin, polyoxymethylene, ethyl cellulose, polyamide nylon 11, polyamide nylon 66, wool and its fabric, silk and its fabric, paper, polyethylene glycol succinate, cellulose, cellulose acetate, polyethylene glycol adipate, polydiallyl phthalate, regenerated cellulose sponge, cotton and its fabric, polyurethane elastomer, styrene-acrylonitrile copolymer, styrene-butadiene copolymer, wood, hard rubber, acetate, rayon, polymethyl methacrylate, polyvinyl alcohol, polyester, copper, aluminum, gold, silver and steel, and the friction material (7) of the first friction member (41) or the second friction member (42) is any one of polystyrene, polyethylene, polypropylene, polydiphenylpropane carbonate, polyethylene terephthalate, polyimide, polyvinyl chloride, polydimethylsiloxane, polychlorotrifluoroethylene and polytetrafluoroethylene.

5. The magnetic bead suspension device according to claim 1, characterized in that: The magnetic bead suspension device further comprises a motor assembly (6), the holding tray (3) being connected to the motor assembly (6) so that the holding tray (3) can rotate, and a receiving groove (31) is provided on the holding tray (3).

6. The magnetic bead suspension device according to claim 1, characterized in that: The reaction container (2) comprises a centrifuge tube or a reagent bottle.

7. The magnetic bead suspension device according to claim 1, characterized in that: When the particle size of the magnetic beads (7) is 1.5 μm, the rotation speed of the driving member (43) is greater than 500 rpm.

8. The magnetic bead suspension device according to claim 1, characterized in that: The second friction member (42) is attached to the support member (44) in a circumferential manner, and the second friction member (42) is in the shape of an inverted triangular prism.

9. The magnetic bead suspension device according to claim 1, characterized in that: The holding tray (3) is installed in an inclined manner in the housing (1), and the angle ɑ between the placement surface of the holding tray (3) and the horizontal plane is in the range of 20°-40°.

10. The magnetic bead suspension device according to claim 1, characterized in that: A guide rail is provided on the inner wall of the shell (1), and the electrostatic generating device (4) is mounted on the guide rail via a mounting member. The distance between the electrostatic generating device (4) and the reaction container (2) can be adjusted along the length direction of the guide rail.

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