Cell culture container

Abstract

The utility model relates to the technical field of cell culture, in particular to a cell culture container which comprises a tank body, a sealing cover is connected to the top of the tank body, an observation window is connected to the front face of the outer wall of the tank body, a stirring mechanism is arranged in the tank body, and a gas injection mechanism is arranged on the back face of the top of the sealing cover. A pressure relief mechanism is arranged on the front face of the top of the sealing cover. By arranging the pressure relief mechanism, when the pressure of the inner wall of the tank body is too high, the purpose of automatic pressure relief can be achieved so that gas generated in the large-batch cultivation process can be conveniently released, and when the internal pressure of the tank body is increased due to the fact that the gas is generated in the large-batch cultivation process, a second spring can be compressed through air pressure to jack an exhaust pipe; when the exhaust hole is exposed out of the upper portion of the sealing cover, the tank body is communicated with the outside, at the moment, redundant gas in the tank body is exhausted to achieve the purpose of automatic pressure relief, or regular pressure relief is conducted in the mode that the push rod is pushed to drive the exhaust pipe to be lifted out of the exhaust hole.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture container. Background Art

[0002] Suspension culture is a technique for culturing cells or organisms in a suspended state within a liquid medium. It is primarily used in cell culture, microbial culture, and plant cell culture. In this culture method, cells or organisms grow freely suspended in a liquid medium, not attached to a solid surface.

[0003] Prior art, such as publication number CN207918876U, provides a cell culture container, including a bottle body, a first branch tube and a second branch tube are respectively provided on both sides above the bottle body, a cover body is provided on the first branch tube, an inflation mechanism is provided on the second branch tube, a fixed block is fixed above the bottle body, a through hole is provided at the center of the fixed block, a fixed cylinder corresponding to the position of the through hole is fixed above the fixed block, a stirring shaft is provided inside the bottle body, the through hole at the upper end of the stirring shaft extends to the inside of the fixed cylinder and is rotatably connected to the inner top of the fixed cylinder. In the present utility model, by providing multiple support plates and multiple circular holes on the support plates, starting the motor can cause the multiple support plates to rotate, and the cell culture fluid can generate vortices through the multiple circular holes to prevent suspended cells from adhering to the inner wall of the bottle body. There is no need to manually shake the bottle body, and it is more convenient to deeply introduce carbon dioxide and air, which greatly improves work efficiency and is highly practical.

[0004] Agitation ensures that cells or organisms in liquid culture media are fully exposed to nutrients, oxygen, and other growth factors, ensuring the uniformity of the culture system. This helps increase cell growth and metabolic activity. Furthermore, gas injection, which supplements the required gas for culture, can increase pressure in the culture vessel. However, in some large-scale microbial fermentation processes, large amounts of gas can be generated, leading to increased pressure within the culture vessel and hindering cell culture. Given this, we propose a cell culture vessel. Utility Model Content

[0005] The purpose of the utility model is to provide a cell culture container, which solves the problem of pressure increase in the culture container.

[0006] To achieve the above objectives, the present invention provides the following technical solutions:

[0007] A cell culture container, comprising a tank body, a sealing cover connected to the top of the tank body, an observation window connected to the front of the outer wall of the tank body, a stirring mechanism provided inside the tank body, a gas injection mechanism provided at the back of the top of the sealing cover, and a pressure relief mechanism provided at the front of the top of the sealing cover;

[0008] The pressure relief mechanism includes a support ring, which is connected to the top front position of the sealing cover through a support rod, a connecting ring is connected to the bottom of the support ring, a second spring is connected to the position between the connecting ring and the support ring, an inner wall of the connecting ring is connected to an exhaust pipe, the exhaust pipe passes through the sealing cover and extends to the interior of the tank body, and is in a sliding connection with the through-hole of the sealing cover, an exhaust hole is opened on the exhaust pipe, and a push rod is connected to the top of the exhaust pipe.

[0009] Preferably, the stirring mechanism includes a driven gear, which is arranged above the sealing cover. The bottom of the driven gear is connected to a hollow rotating rod, which passes through the sealing cover and extends to the interior of the tank body, and is rotatably connected to the penetration point of the sealing cover. The outer wall of the hollow rotating rod is connected to a stirring blade.

[0010] Preferably, one side of the driven gear is meshedly connected with a driving gear, and the top of the sealing cover is connected to a motor for driving the driving gear to rotate.

[0011] Preferably, one end of the bottom of the hollow rotating rod is connected to a rotating plate, the top of the rotating plate is connected to an air injection hole, the air injection hole is connected to the interior of the hollow rotating rod, and the top of the driven gear is connected to a touch ball.

[0012] Preferably, the gas injection mechanism includes a support frame, which is connected to the back of the top of the sealing cover. The inner wall of the support frame is connected to a connector, which is rotatably connected to one end of the top of the hollow rotating rod and is connected to the interior of the hollow rotating rod.

[0013] Preferably, an air injection cylinder is connected to the inner wall of the support frame, and the air injection cylinder is connected to the connecting head through a connecting pipe.

[0014] Preferably, a piston is slidably connected to the inner wall of the gas injection cylinder, a first spring is connected to the position between the piston and the inner wall of the gas injection cylinder, and a one-way valve is connected to the top of the gas injection cylinder.

[0015] By means of the above technical solution, the present invention provides a cell culture container having at least the following beneficial effects:

[0016] 1. The utility model is provided with a pressure relief mechanism, which can achieve the purpose of automatic pressure relief when the pressure on the inner wall of the tank is too high, so as to facilitate the release of gas generated during the large-scale cultivation process. When the tank generates gas due to the large-scale cultivation process, resulting in an increase in internal pressure, the ventilation pressure can compress the second spring to lift the exhaust pipe. When the exhaust hole is exposed above the sealing cover, the tank is connected to the outside. At this time, the excess gas inside the tank is discharged to achieve the purpose of automatic pressure relief, or regular pressure relief can be performed by pushing the push rod to lift the exhaust pipe to expose the exhaust hole.

[0017] 2. During the rotation of the touch ball of the utility model, it will contact the piston and lift the piston. The air in the gas injection cylinder is squeezed into the connecting tube through the piston, and is injected into the hollow rotating rod through the connecting head, and then discharged from the gas injection hole on the rotating plate, so as to facilitate the replenishment of the gas required by the cells or organisms in the culture medium, so as to increase the growth rate and metabolic activity of the cells. When the touch ball leaves the piston, the tension of the first spring will push the piston to reset and suck in new air holes from the one-way valve, thereby achieving the purpose of intermittent gas injection. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The accompanying drawings described herein are used to provide a further understanding of the present invention and constitute a part of this application:

[0019] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0020] Figure 2 It is a partial cross-sectional view of the utility model;

[0021] Figure 3 For this utility model Figure 2 The structure of the driven gear is shown in FIG.

[0022] Figure 4 It is a partial cross-sectional view of the gas injection cylinder in the utility model.

[0023] In the figure: 1. Tank body; 11. Observation window; 12. Sealing cover; 2. Stirring mechanism; 21. Driven gear; 211. Touch ball; 22. Hollow rotating rod; 23. Stirring blade; 24. Driving gear; 25. Motor; 26. Rotating plate; 261. Air injection hole; 3. Air injection mechanism; 31. Support frame; 32. Air injection cylinder; 321. Piston; 322. First spring; 323. One-way valve; 33. Connector; 331. Connecting pipe; 4. Pressure relief mechanism; 41. Support ring; 411. Support rod; 412. Second spring; 42. Connecting ring; 43. Exhaust pipe; 431. Exhaust hole; 44. Push rod. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0025] Example 1

[0026] A cell culture container, such as Figure 1 、 Figure 2 、 Figure 3 As shown, it includes a tank body 1, a sealing cover 12 is connected to the top of the tank body 1, an observation window 11 is connected to the front of the outer wall of the tank body 1, a stirring mechanism 2 is arranged inside the tank body 1, a gas injection mechanism 3 is arranged at the position of the top back of the sealing cover 12, and a pressure relief mechanism 4 is arranged at the position of the top front of the sealing cover 12, wherein the pressure relief mechanism 4 includes a support ring 41, the support ring 41 is connected to the position of the top front of the sealing cover 12 through a support rod 411, a connecting ring 42 is connected to the bottom of the support ring 41, a second spring 412 is connected to the position between the connecting ring 42 and the support ring 41, an inner wall of the connecting ring 42 is connected to an exhaust pipe 43, the exhaust pipe 43 passes through the sealing cover 12 and extends to the interior of the tank body 1, and is in sliding connection with the through-hole of the sealing cover 12, an exhaust hole 431 is opened on the exhaust pipe 43, and a push rod 44 is connected to the top of the exhaust pipe 43.

[0027] In this embodiment, by providing a pressure relief mechanism 4, when the pressure on the inner wall of the tank body 1 is too high, the pressure can be automatically relieved, thereby facilitating the release of gas generated during the large-scale cultivation process.

[0028] More specifically, when the tank body 1 generates gas due to a large-scale cultivation process, causing the internal pressure to increase, the ventilation pressure can be used to compress the second spring 412 to lift the exhaust pipe 43. When the exhaust hole 431 is exposed above the sealing cover 12, the tank body 1 is connected to the outside. At this time, the excess gas inside the tank body 1 is discharged to achieve the purpose of automatic pressure relief, or regular pressure relief can be performed by pushing the push rod 44 to lift the exhaust pipe 43 to expose the exhaust hole 431.

[0029] Example 2

[0030] like Figure 1 、 Figure 2As shown, the stirring mechanism 2 includes a driven gear 21, which is arranged above the sealing cover 12. The bottom of the driven gear 21 is connected to a hollow rotating rod 22, which passes through the sealing cover 12 and extends to the interior of the tank body 1, and is rotatably connected to the penetration point of the sealing cover 12. The outer wall of the hollow rotating rod 22 is connected to a stirring blade 23, and one side of the driven gear 21 is meshed with a driving gear 24. The top of the sealing cover 12 is connected to a motor 25 for driving the driving gear 24 to rotate, one end of the bottom of the hollow rotating rod 22 is connected to a rotating plate 26, and the top of the rotating plate 26 is connected to an air injection hole 261. The air injection hole 261 is communicated with the interior of the hollow rotating rod 22, and the top of the driven gear 21 is connected to a touch ball 211.

[0031] In this embodiment, a starting motor 25 is provided to drive the driving gear 24 to rotate, and the meshing driven gear 21 drives the stirring blade 23 on the hollow rotating rod 22 to rotate, so as to facilitate the stirring of the culture medium inside the tank body 1. Through stirring, the cells or organisms in the culture medium can fully contact with nutrients, oxygen and other growth factors, thereby ensuring the uniformity of the culture system, which helps to improve the growth rate and metabolic activity of the cells.

[0032] It is worth noting that when the driven gear 21 rotates, it also drives the touch ball 211 to rotate together. During the rotation of the touch ball 211, it intermittently pushes the push rod 44 to drive the exhaust pipe 43 to lift up and expose the exhaust hole 431, thereby achieving the purpose of regular pressure relief.

[0033] Example 3

[0034] like Figure 1 - Figure 4 As shown, the gas injection mechanism 3 includes a support frame 31, which is connected to the top back of the sealing cover 12, and the inner wall of the support frame 31 is connected to a connector 33, which is rotatably connected to one end of the top of the hollow rotating rod 22 and is connected to the interior of the hollow rotating rod 22, and the inner wall of the support frame 31 is connected to a gas injection cylinder 32, which is connected to the connector 33 through a connecting pipe 331, and the inner wall of the gas injection cylinder 32 is slidably connected to a piston 321, and a first spring 322 is connected to the position between the piston 321 and the inner wall of the gas injection cylinder 32, and a one-way valve 323 is connected to the top of the gas injection cylinder 32.

[0035] In this embodiment, during the rotation of the touch ball 211, it will contact the piston 321, which will lift the piston 321, and the air in the gas injection cylinder 32 will be squeezed into the connecting tube 331 through the piston 321, and injected into the hollow rotating rod 22 through the connecting head 33, and then discharged from the gas injection hole 261 on the rotating plate 26, so as to facilitate the replenishment of the gas required by the cells or organisms in the culture medium to increase the growth rate and metabolic activity of the cells. When the touch ball 211 leaves the piston 321, the tension of the first spring 322 will push the piston 321 to reset, and suck in new air holes from the one-way valve 323, thereby achieving the purpose of intermittent gas injection.

[0036] When a cell culture container of the present invention is in use, the starting motor 25 drives the driving gear 24 to rotate, and the meshed driven gear 21 drives the stirring blade 23 on the hollow rotating rod 22 to rotate, so as to facilitate the stirring of the culture liquid inside the tank body 1. Through stirring, the cells or organisms in the culture liquid can fully contact with nutrients, oxygen and other growth factors, ensuring the uniformity of the culture system, which helps to improve the growth rate and metabolic activity of the cells. When the tank body 1 produces gas due to a large-scale cultivation process, resulting in an increase in internal pressure, the ventilation pressure can be used to compress the second spring 412 to lift the exhaust pipe 43. When the exhaust hole 431 is exposed above the sealing cover 12, the tank body 1 is connected to the outside. At this time, the excess gas inside the tank body 1 is discharged to achieve the purpose of automatic pressure relief, or the driven gear 21 rotates. When the touch ball 211 rotates, it drives the touch ball 211 to rotate together. During the rotation of the touch ball 211, it will intermittently push the push rod 44 to drive the exhaust pipe 43 to lift and expose the exhaust hole 431, so as to achieve the purpose of regular pressure relief. During the rotation of the touch ball 211, it will contact the piston 321, and will lift the piston 321. The air in the gas injection cylinder 32 is squeezed into the connecting tube 331 through the piston 321, and is injected into the hollow rotating rod 22 through the connecting head 33, and then discharged from the gas injection hole 261 on the rotating plate 26, so as to facilitate the replenishment of the gas required by the cells or organisms in the culture medium to increase the growth rate and metabolic activity of the cells. When the touch ball 211 leaves the piston 321, the tension of the first spring 322 will push the piston 321 to reset and inhale new air holes from the one-way valve 323, so as to achieve the purpose of intermittent gas injection.

[0037] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0038] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell culture container, comprising a tank body (1), characterized in that: The top of the tank body (1) is connected to a sealing cover (12), the front of the outer wall of the tank body (1) is connected to an observation window (11), a stirring mechanism (2) is provided inside the tank body (1), a gas injection mechanism (3) is provided at the top back of the sealing cover (12), and a pressure relief mechanism (4) is provided at the top front of the sealing cover (12); The pressure relief mechanism (4) comprises a support ring (41), the support ring (41) is connected to the top front position of the sealing cover (12) through a support rod (411), the bottom of the support ring (41) is connected to a connecting ring (42), a second spring (412) is connected to the position between the connecting ring (42) and the support ring (41), the inner wall of the connecting ring (42) is connected to an exhaust pipe (43), the exhaust pipe (43) passes through the sealing cover (12) and extends to the interior of the tank body (1), and is in sliding connection with the sealing cover (12), the exhaust pipe (43) is provided with an exhaust hole (431), and the top of the exhaust pipe (43) is connected to a push rod (44).

2. A cell culture container according to claim 1, characterized in that: The stirring mechanism (2) comprises a driven gear (21), the driven gear (21) being arranged above the sealing cover (12), the bottom of the driven gear (21) being connected to a hollow rotating rod (22), the hollow rotating rod (22) penetrating the sealing cover (12) and extending to the interior of the tank body (1), and being rotatably connected to the penetration point of the sealing cover (12), and the outer wall of the hollow rotating rod (22) being connected to a stirring blade (23).

3. A cell culture container according to claim 2, characterized in that: One side of the driven gear (21) is meshedly connected with a driving gear (24), and the top of the sealing cover (12) is connected with a motor (25) for driving the driving gear (24) to rotate.

4. A cell culture container according to claim 3, characterized in that: One end of the bottom of the hollow rotating rod (22) is connected to a rotating plate (26), the top of the rotating plate (26) is connected to an air injection hole (261), the air injection hole (261) is communicated with the interior of the hollow rotating rod (22), and the top of the driven gear (21) is connected to a touch ball (211).

5. The cell culture container according to claim 1, wherein: The gas injection mechanism (3) includes a support frame (31), the support frame (31) is connected to the top back of the sealing cover (12), the inner wall of the support frame (31) is connected to a connector (33), the connector (33) is rotatably connected to one end of the top of the hollow rotating rod (22), and is communicated with the interior of the hollow rotating rod (22).

6. The cell culture container according to claim 5, characterized in that: An air injection cylinder (32) is connected to the inner wall of the support frame (31), and the air injection cylinder (32) is connected to the connector (33) via a connecting pipe (331).

7. The cell culture container according to claim 6, characterized in that: The inner wall of the gas injection cylinder (32) is slidably connected to a piston (321), a first spring (322) is connected between the piston (321) and the inner wall of the gas injection cylinder (32), and a one-way valve (323) is connected to the top of the gas injection cylinder (32).

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