Blood type microcolumn gel card

Abstract

The utility model relates to the technical field of medical instruments, in particular to a blood type micro-column gel card, which comprises a card body and a plurality of gel tubes arranged and fixed on the card body at equal intervals, and is characterized in that each gel tube is provided with a sample adding cavity, a reaction cavity and a gel column which are sequentially connected from top to bottom; erythrocytes can be injected from the sample adding cavity and fall into the gel column after reacting in the reaction cavity to realize blood type detection, and the detection mode is convenient; moreover, a collecting tube for negative red blood cells to pass through is arranged at the bottom of the gel column, so that when the detection result is double groups, the negative red blood cells which are not combined with the blood group antibody in the gel column can fall into the collecting tube through a gel gap to be measured, and the positive red blood cells are blocked and retained at the upper part of the gel column; therefore, separation of positive red blood cells and negative red blood cells is achieved, further interpretation of double-group results is achieved, the information amount of one-time detection is increased, and the detection efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical devices, in particular to a blood type microcolumn gel card. Background Art

[0002] The blood type microcolumn gel card adds red blood cells diluted to a concentration of about 1% into a test tube, allowing the red blood cells to combine with the corresponding blood type antibodies to form red blood cell agglutination complexes. Under a certain centrifugal force, the dispersed red blood cells pass through the gel gaps and settle at the bottom of the test well, while the agglutination complexes cannot pass through the gel gaps and float on the gel surface or suspend in the gel, thereby achieving blood type detection and obtaining positive, negative and double-group interpretation results of varying intensities.

[0003] However, for dual-group results, distinguishing the ratio of positive red blood cells to negative red blood cells in the dual-group results is of great significance for the identification of blood type subtypes and the true blood type of patients after non-homotype blood transfusions. However, the blood type microcolumn gel card in the existing technology cannot determine the ratio of positive red blood cells to negative red blood cells in the dual-group results, resulting in a small amount of information obtained in one test, requiring further testing, and low efficiency. Utility Model Content

[0004] In order to solve the problems in the above-mentioned background technology, the utility model provides a blood type microcolumn gel card, which can further judge the ratio of positive red blood cells to negative red blood cells when the detection result is a double group, thereby increasing the amount of information obtained in one test and thus improving the detection efficiency.

[0005] The solution adopted by the utility model to solve the technical problem is: a blood type microcolumn gel card, including a card body and a plurality of gel tubes equidistantly arranged and fixed on the card body, the gel tubes including sample loading chambers and gel columns for loading gel beads and corresponding blood type antibodies arranged in sequence from top to bottom, the bottom of the gel column is connected to a collection tube for collecting negative red blood cells in the dual-group results, and the collection tube is connected to the gel column.

[0006] Furthermore, the diameter of the collection tube is between the diameter of the gel beads and the diameter of the red blood cells.

[0007] Furthermore, the diameter of the collecting tube is 30-40 μm.

[0008] Furthermore, the surface of the collecting tube is provided with a scale.

[0009] Furthermore, the cross section of the inner cavity of the collecting tube is rectangular, elliptical or circular.

[0010] Furthermore, the projection surface of the card body is a "U"-shaped structure, including a horizontally arranged clamping portion and connecting arms symmetrically arranged on both sides of the clamping portion. The two connecting arms are combined to form a connecting space, and several gel tubes are equidistantly connected side by side in the connecting space. The gel tubes located at both ends are respectively connected to the two connecting arms.

[0011] Furthermore, the surface of the sample adding cavity is provided with reinforcing ribs.

[0012] Furthermore, a tearable or penetrable sealing film is attached to the opening of the sample adding cavity.

[0013] Furthermore, the card body and the gel tube are both made of plastic and are integrally formed.

[0014] In summary, the beneficial effects of the present invention are as follows: the present invention arranges the gel tube as a sample loading chamber, a reaction chamber and a gel column connected in sequence from top to bottom, so that red blood cells can be injected from the sample loading chamber and fall into the gel column after reacting in the reaction chamber to realize blood type detection, and the detection method is convenient; and, by arranging a collection tube for passing negative red blood cells at the bottom of the gel column, when the test result is a double group, the negative red blood cells in the gel column that are not bound to the blood type antibodies can fall into the collection tube through the gel gap, while the positive red blood cells are agglutinated by the corresponding blood type antibodies and are blocked and retained in the gel column, thereby realizing the separation of positive red blood cells and negative red blood cells, and then realizing further interpretation of the double group results, increasing the amount of information in one test, and improving the detection efficiency.

[0015] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention, it can be implemented in accordance with the contents of the specification. In addition, in order to make the above and other purposes, features and advantages of the present invention more obvious and easy to understand, the following preferred embodiments are specifically cited and described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 Schematic diagram of the structure of this embodiment;

[0017] Figure 2 is a cross-sectional view of this embodiment;

[0018] Figure 3 for Figure 1 Enlarged view of point A in the middle.

[0019] In the figure: 1. card body; 11. clamping part; 12. connecting arm; 2. gel tube; 21. sample adding chamber; 211. reinforcing rib; 212. sealing membrane; 22. gel column; 23. collecting tube; 3. scale. DETAILED DESCRIPTION

[0020] In order to make the content of the present invention more clearly understood, the present invention will be further described below based on specific embodiments in conjunction with the accompanying drawings.

[0021] It should be noted that the terms "center," "upper," "lower," "front," "back," "left," "right," "inner," and "outer" used herein to indicate positions or locations are based on the positions or locations shown in the accompanying drawings and are intended solely to facilitate and simplify the description of the present invention. They are not intended to indicate or imply that the devices or components referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on the present invention. Unless otherwise specified, "plurality" means two or more.

[0022] Unless otherwise specified or limited, the terms "mounted," "connected," and "connected" should be understood broadly. For example, they can refer to fixed, removable, or integral connections; mechanical or electrical connections; direct or indirect connections through an intermediary; and internal communication between two components. Those skilled in the art will understand the specific meanings of these terms in this utility model based on specific circumstances.

[0023] like Figures 1 to 2 The present invention discloses a blood type microcolumn gel card that can detect blood type and further interpret the ratio of positive to negative red blood cells when a double-group result is detected, thereby increasing the amount of information obtained from a single test and improving detection efficiency. The blood type microcolumn gel card of this embodiment comprises a card body 1 and a plurality of gel tubes 2 equidistantly arranged and fixed to the card body 1. The gel tubes 2 contain gel beads and blood type antibodies corresponding to the blood type. By adding red blood cells diluted to a concentration of approximately 1% into the gel tubes 2 and then attaching the gel tubes 2 to a centrifuge via the card body 1, the positive red blood cells are agglutinated by the corresponding blood type antibodies to form clots, which are then trapped and retained in the upper portion of the gel column 22. Dispersed negative red blood cells, which lack the corresponding antigens to bind to the blood type antibodies, pass through the gel gaps under the action of a certain centrifugal force and are deposited at the bottom of the gel tubes 2, thereby achieving blood type detection.

[0024] Specifically, the gel tube 2 of this embodiment includes a sample loading chamber 21 and a gel column 22 arranged in sequence from top to bottom. Red blood cells diluted to a concentration of approximately 1% can enter the gel tube 2 through the sample loading chamber 21 and react with blood type antibodies in the gel column 22. Subsequently, under the action of centrifugal force, red blood cells with corresponding antigens combine with blood type antibodies to form red blood cell agglutination complexes, which are blocked and retained in the upper part of the gel column 22. The dispersed red blood cells that have not reacted with the blood type antibodies are deposited at the bottom of the gel tube 2 under the action of centrifugal force, completing the detection and identification of blood type.

[0025] like Figure 1 and Figure 2 As shown, to further determine the proportion of negative red blood cells in the dual-population results that are unbound by blood group antibodies, this embodiment provides a collection tube 23 at the bottom of the gel column 22 for collecting negative red blood cells in the dual-population results. Collection tube 23 is connected to gel column 22, and its diameter is between the diameter of the gel beads and the diameter of the red blood cells. Negative red blood cells dispersed in the dual-population results can be deposited at the bottom of gel tube 2 under the action of centrifugal force and enter collection tube 23. Positive red blood cells that have reacted with blood group antibodies form larger clots and remain within gel tube 2, achieving separation of positive and negative red blood cells, thereby facilitating the interpretation of the positive-to-negative ratio in the dual-population results.

[0026] Specifically, since the diameter of gel beads is typically 70-80 μm, while the diameter of negative red blood cells that have not reacted with blood group antibodies is typically 7-8 μm, the diameter of the collection tube 23 in this embodiment is set to 30-40 μm, between the gel beads and the red blood cells. This allows the unreacted negative red blood cells to enter the collection tube 23 under the action of centrifugal force, while the positive red blood cells that have reacted with the blood group antibodies form larger clots that remain in the gel tube 2 and cannot enter the collection tube 23. This achieves separation of positive and negative red blood cells, facilitating the determination of the ratio between the two.

[0027] When performing blood typing, one of the gel tubes 2 can be set up without blood typing antibodies as a negative control group, thereby serving as a reference standard for uniformly loading the red blood cells in each gel tube 2. After centrifugation, the ratio of the negative red blood cells in each collection tube 23 can be compared with the ratio of the negative red blood cells in the negative control group to calculate the ratio of negative red blood cells to positive red blood cells.

[0028] like Figure 1 and Figure 3 As shown, the surface of the collection tube 23 of this embodiment is also provided with a scale 3, which is convenient for the staff to read the amount of negative red blood cells entering the collection tube 23, and can improve the reading accuracy, thereby improving the overall detection accuracy.

[0029] Furthermore, the cross-section of the inner cavity of the collecting tube 23 of this embodiment includes but is not limited to rectangular, elliptical or circular, which can achieve the separation of positive red blood cells from negative red blood cells and is convenient for manufacturers to produce according to actual conditions.

[0030] like Figure 1 and Figure 2As shown, the projection surface of the card body 1 of this embodiment is a "U"-shaped structure, including a horizontally arranged clamping portion 11 and connecting arms 12 symmetrically arranged on both sides of the clamping portion 11. The two connecting arms 12 of this embodiment are combined to form a connecting space, and each gel tube 2 can be connected in parallel at equal distances in the connecting space, and the gel tubes 2 at both ends are respectively connected to the two connecting arms 12, so that after this embodiment is installed in the card seat of the centrifuge through the clamping portion 11, each gel tube 2 can be placed horizontally in sequence, thereby facilitating the separation of positive red blood cells and negative red blood cells by the centrifuge to achieve accurate detection.

[0031] like Figure 1 As shown, further, the gel tube 2 of this embodiment is connected to the connecting arm 12 of the card body 1 in sequence through the sample loading cavity 21. In order to improve the connection strength and maintain the overall stability of the blood type microcolumn gel card, this embodiment provides a reinforcing rib 211 on the surface of the sample loading cavity 21, which can prevent the connection position of the sample loading cavity 21 from being bent or broken during the centrifugation process, resulting in errors in the test results, further improving the blood type detection accuracy of this embodiment.

[0032] And, as Figure 1 As shown, a tearable or penetrable sealing film 212 is attached to the top opening of the sample loading chamber 21 of this embodiment, which can seal the inner cavity of the gel tube 2. The film can be torn or penetrated during testing to prevent the gel tube 2 from being contaminated and causing deviations in the test results, thereby further improving the accuracy of the test.

[0033] In addition, the card body 1 and the gel tube 2 of this embodiment are both made of plastic and integrally formed, which can effectively reduce production costs and facilitate production. The plastic is transparent, making it easy to read the amount of negative red blood cells that fall into the collection tube 23 after separation.

[0034] In summary, the working principle of this embodiment is as follows: this embodiment arranges the gel tube 2 as a sample loading chamber 21, a reaction chamber and a gel column 22 connected in sequence from top to bottom, so that red blood cells can be injected from the sample loading chamber 21 and fall into the gel column 22 after reacting in the reaction chamber to realize blood type detection, and the detection method is convenient; and, by arranging a collection tube 23 for passing negative red blood cells at the bottom of the gel column 22, when the test result is a double group, the negative red blood cells in the gel column 22 that are not bound to the blood type antibodies can fall into the collection tube 23 through the gel gap, while the positive red blood cells form clots due to the aggregation of antibodies and are blocked and retained in the upper part of the gel column 22, thereby realizing the separation of positive red blood cells and negative red blood cells, and by measuring the amount of red blood cells in the collection tube 23, further interpretation of the double group results is realized, thereby increasing the amount of information in one test and improving the detection efficiency.

[0035] The embodiments described above are only preferred implementation methods of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantial changes and modifications made by technicians in this field on the basis of the utility model shall fall within the scope of protection of the present invention.

Claims

1. A blood type microcolumn gel card, comprising a card body (1) and a plurality of gel tubes (2) equidistantly arranged and fixed on the card body (1), characterized in that: The gel tube (2) comprises a sample loading chamber (21) and a gel column (22) for loading gel beads and corresponding blood type antibodies, which are arranged in sequence from top to bottom. The bottom of the gel column (22) is connected to a collection tube (23) for collecting negative red blood cells in the dual-group results, and the collection tube (23) is communicated with the gel column (22).

2. The blood type microcolumn gel card according to claim 1, characterized in that: The diameter of the collecting tube (23) is between the diameter of the gel beads and the diameter of the red blood cells.

3. The blood type microcolumn gel card according to claim 2, characterized in that: The diameter of the collecting tube (23) is 30-40 μm.

4. The blood type microcolumn gel card according to claim 1, characterized in that: The surface of the collecting tube (23) is provided with a scale (3).

5. The blood type microcolumn gel card according to claim 1, characterized in that: The cross section of the inner cavity of the collecting tube (23) is rectangular, elliptical or circular.

6. The blood type microcolumn gel card according to claim 1, characterized in that: The projection surface of the card body (1) is a "U"-shaped structure, comprising a horizontally arranged clamping portion (11) and connecting arms (12) symmetrically arranged on both sides of the clamping portion (11); the two connecting arms (12) are combined to form a connecting space; a plurality of gel tubes (2) are equidistantly connected side by side in the connecting space; the gel tubes (2) located at both ends are respectively connected to the two connecting arms (12).

7. The blood type microcolumn gel card according to claim 1, characterized in that: The surface of the sample adding cavity (21) is provided with reinforcing ribs (211).

8. The blood type microcolumn gel card according to claim 1, characterized in that: A tearable or penetrable sealing film (212) is attached to the opening of the sample adding cavity (21).

9. The blood type microcolumn gel card according to any one of claims 1 to 8, characterized in that: The card body (1) and the gel tube (2) are both made of plastic and are integrally formed.

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