Pipetting mechanism of western blot instrument

Abstract

The utility model provides a pipetting mechanism of a western blot instrument. Relates to the technical field of protein analysis auxiliary tools. The pipetting mechanism of the western blot instrument comprises an outer portal frame and an inner portal frame, the inner portal frame is located in the outer portal frame, a plurality of liquid suction barrels are arranged in the inner portal frame, liquid suction needles are fixedly installed at the bottoms of the liquid suction barrels, pushing plugs are arranged in the liquid suction barrels, and the pushing plugs are connected with the liquid suction barrels. Pull rods are fixedly installed at the tops of the multiple pushing plugs, the top ends of the multiple pull rods extend to the positions above the multiple liquid suction barrels correspondingly, an electric push rod is fixedly installed at the top of the inner portal frame, and the electric push rod penetrates through the top of the outer portal frame and is movably connected with the top of the outer portal frame. The liquid transfer device has the advantages that liquid in a plurality of test tubes can be transferred at one time, and the working efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of protein analysis auxiliary tools, in particular to a liquid transfer mechanism of a protein blotting instrument. Background Art

[0002] A protein blotting instrument is a device used to achieve automated protein separation and immunoassay in protein blotting. It can automatically complete the separation, immunoassay, qualitative and quantitative analysis of protein samples. It is widely used in many fields such as protein property identification, protein function research, and antibody research. During use, a pipette is required to transfer the liquid required for the detection process.

[0003] However, most common pipettes extract liquid one at a time, and most of them are single-unit operations. When liquid in a row of test tubes needs to be extracted and transferred, multiple back-and-forth aspiration and expulsion are required, thereby reducing the working efficiency of the protein blotting instrument.

[0004] Therefore, it is necessary to provide a new liquid transfer mechanism for a protein blotting instrument to solve the above technical problems. Utility Model Content

[0005] The utility model aims to provide a liquid transfer mechanism for a protein blotting instrument which can transfer liquids in a plurality of test tubes at one time and improve the working efficiency.

[0006] In order to solve the above technical problems, the present invention provides a pipetting mechanism for a protein blotting instrument, comprising: an outer gantry and an inner gantry, wherein the inner gantry is located inside the outer gantry, and a plurality of liquid-absorbing buckets are arranged inside the inner gantry, and a plurality of liquid-absorbing buckets are fixedly installed with liquid-absorbing needles at the bottoms thereof, and a plurality of liquid-absorbing buckets are provided with push plugs, and a plurality of push plugs are fixedly installed with pull rods at the tops thereof, and the tops of the plurality of pull rods extend respectively to the tops of the plurality of liquid-absorbing buckets, and an electric push rod is fixedly installed on the top of the inner gantry, and the electric push rod passes through the inner gantry. The top of the outer gantry is movably connected to the top of the outer gantry, the output shaft of the electric push rod passes through the top of the inner gantry and is slidably connected to the top of the inner gantry, the output shaft of the electric push rod is fixedly installed with a mounting frame, a supporting slide rod is fixedly installed in the mounting frame, a plurality of slide seats are slidably installed on the supporting slide rod, the top ends of the plurality of pull rods are respectively fixedly connected to the plurality of slide seats, and the plurality of slide seats are all threaded with second fastening screws, and the ends of the plurality of second fastening screws are all in conflict with the support slide rod.

[0007] Preferably, vertical sliding columns are fixedly installed on the inner walls on both sides of the outer gantry, and the top ends of the two vertical sliding columns are fixedly connected to the top inner wall of the outer gantry. Sliding arms are slidably installed on the two vertical sliding columns, and the sides of the two sliding arms close to each other are respectively fixedly connected to the outer walls on both sides of the inner gantry. First fastening screws are threadedly installed on the two sliding arms, and the ends of the two first fastening screws respectively conflict with the two vertical sliding columns.

[0008] Preferably, two cross arms are fixedly installed in the inner gantry, and connecting blocks are slidably installed on the two cross arms, and the corresponding two connecting blocks are fixedly connected to the corresponding liquid suction buckets.

[0009] Preferably, an adjusting screw is threadedly installed on the top of the inner gantry, and a positioning plate is rotatably installed on the bottom end of the adjusting screw. Two limit columns are slidably installed on the top of the inner gantry, and the bottom ends of the two limit columns are fixedly connected to the mounting frame. One of the limit columns is engraved with scale lines, and a pointing piece is fixedly installed on the side of the positioning plate close to the limit column, and the pointing piece is adapted to the scale lines.

[0010] Preferably, a movable opening is provided on the top of the outer gantry, and the electric push rod and the adjusting screw both pass through the movable opening.

[0011] Preferably, a scale located above the slide is fixedly installed in the installation frame, and a plurality of guide headers are fixedly installed on the tops of the slides, and the plurality of guide headers are adapted to the scale.

[0012] Compared with related technologies, the pipetting mechanism of the protein blotting instrument provided by the present invention has the following beneficial effects:

[0013] The utility model provides a liquid transfer mechanism for a protein blotting instrument. By providing a plurality of liquid suction buckets, liquids in a plurality of test tubes in a row can be transferred, thereby greatly improving work efficiency. Moreover, when transferring liquids, the content of the body fluid that can be absorbed can be adjusted by adjusting the position of the positioning plate, thereby improving work efficiency while ensuring the accurate content of the transferred liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 A schematic structural diagram of a preferred embodiment of the liquid transfer mechanism of the protein blotting instrument provided by the present invention;

[0015] Figure 2 This is a schematic diagram of the connection structure of the vertical sliding column in the present utility model;

[0016] Figure 3 This is a schematic diagram of the assembly of the inner gantry and the mounting frame in the present invention;

[0017] Figure 4 This is a schematic diagram of the assembly of the cross arm and the connecting block in the utility model;

[0018] Figure 5 It is a schematic cross-sectional structural diagram of the liquid suction barrel in the present utility model.

[0019] Numbers in the figure: 1. External gantry; 2. Vertical sliding column; 3. Sliding arm; 4. Internal gantry; 5. First fastening screw; 6. Cross arm; 7. Connecting block; 8. Liquid suction bucket; 9. Liquid suction needle; 10. Pull rod; 11. Push plug; 12. Mounting frame; 13. Electric push rod; 14. Support slide rod; 15. Sliding seat; 16. Second fastening screw; 17. Adjusting screw; 18. Positioning plate; 19. Pointing piece. DETAILED DESCRIPTION

[0020] The present invention will be further described below with reference to the accompanying drawings and implementation examples.

[0021] Please refer to Figure 1-Figure 5 ,in, Figure 1 A schematic structural diagram of a preferred embodiment of the liquid transfer mechanism of the protein blotting instrument provided by the present invention; Figure 2 This is a schematic diagram of the connection structure of the vertical sliding column in the present utility model; Figure 3 This is a schematic diagram of the assembly of the inner gantry and the mounting frame in the present invention; Figure 4 This is a schematic diagram of the assembly of the cross arm and the connecting block in the utility model;

[0022] Figure 5The figure is a schematic diagram of the cross-sectional structure of the liquid-pipetting barrel in the present invention. The liquid-pipetting mechanism of the protein blotting instrument includes: an outer gantry 1 and an inner gantry 4. The inner gantry 4 is located inside the outer gantry 1. A plurality of liquid-pipetting barrels 8 are provided in the inner gantry 4. Liquid-pipetting needles 9 are fixedly installed at the bottom of the plurality of liquid-pipetting barrels 8. Push plugs 11 are provided in the plurality of liquid-pipetting barrels 8. Pull rods 10 are fixedly installed on the tops of the plurality of push plugs 11. When aspirating liquid, the liquid-pipetting needles 9 are first inserted into the solution in the test tube, and then the liquid can be aspirated by stretching the pull rods 10. The top ends of the plurality of pull rods 10 extend to the tops of the plurality of liquid-pipetting barrels 8 respectively. An electric push rod 13 is fixedly installed on the top of the inner gantry 4. The electric push rod 13 passes through the top of the outer gantry 1 and is movably connected to the top of the outer gantry 1. The output shaft of the electric push rod 13 passes through the top of the inner gantry 4 and is slidably connected to the top of the inner gantry 4. A mounting frame 12 is fixedly installed on the output shaft of the electric push rod 13, and a supporting slide 14 is fixedly installed therein, and a plurality of slides 15 are slidably installed on the supporting slide 14. The top ends of the plurality of pull rods 10 are respectively fixedly connected to the plurality of slides 15, and the plurality of slides 15 are all threadedly installed with second fastening screws 16. The ends of the plurality of second fastening screws 16 are all in conflict with the support slide 14. By screwing the second fastening screws 16, the clamping force between the slide 15 and the support slide 14 can be removed, so that the position of the liquid suction bucket 8 can be adjusted, and it can be adjusted according to the actual test tube position, with high flexibility.

[0023] In the above method, in order to enable the pipette needle 9 to be inserted into the test tube, vertical sliding columns 2 are fixedly installed on the inner walls on both sides of the outer gantry 1, and the top ends of the two vertical sliding columns 2 are fixedly connected to the top inner wall of the outer gantry 1, and sliding arms 3 are slidably installed on the two vertical sliding columns 2, and the sides of the two sliding arms 3 close to each other are respectively fixedly connected to the outer walls on both sides of the inner gantry 4, and the two sliding arms 3 are threadedly installed with first fastening screws 5, and the ends of the two first fastening screws 5 are respectively in conflict with the two vertical sliding columns 2. By screwing the first fastening screws 5, the sliding arms 3 can slide up and down freely.

[0024] In this method, in order to ensure that the liquid suction bucket 8 can only move horizontally, two cross arms 6 are fixedly installed in the inner gantry 4, and connecting blocks 7 are slidably installed on the two cross arms 6. The corresponding two connecting blocks 7 are fixedly connected to the corresponding liquid suction bucket 8.

[0025] The bottom end of the lever 18 is fixedly mounted on the top of the lever 12 so that the lever 12 can be adjusted to its desired position.

[0026] In this method, in order to accurately adjust the distance between two adjacent liquid suction buckets 8, a scale is fixedly installed above the slide 15 in the mounting frame 12, and multiple guide heads are fixedly installed on the top of multiple slides 15, and multiple guide heads are adapted to the scale.

[0027] The working principle of the liquid transfer mechanism of the protein blotting instrument provided by the utility model is as follows:

[0028] In this mechanism, the output shaft of the electric push rod 13 is initially in an extended state;

[0029] When it is necessary to transfer the liquid in a row of multiple test tubes, first place the outer gantry 1 in the designated position so that the pipette bucket 8 is above the test tube, and then adjust the distance between the multiple pipette buckets 8 according to the spacing between the two adjacent test tubes. During the adjustment, except for the second fastening screw 16 in the middle, which remains stationary, the other second fastening screws 16 are unscrewed so that the corresponding number of slides 15 can slide again, and then the slide 15 closest to the middle slide 15 is dialed. At this time, observe the direction between the corresponding index header and the scale, adjust it to the designated position, tighten the corresponding second fastening screw 16, and then adjust the other slides 15 in turn according to this method. After the adjustment is completed, the spacing between the two adjacent pipette buckets 8 is adapted to the spacing between the two adjacent test tubes.

[0030] Then, screw the two first fastening screws 5 to release the clamping force on the sliding arm 3, and then push the inner gantry 4 downward to insert the multiple aspiration needles 9 into the corresponding test tubes. Then, start the output shaft of the electric push rod 13 to retract and start the aspiration work.

[0031] After the aspiration is completed, the inner gantry 4 is moved upwards, the first fastening screw 5 is re-tightened, and the outer gantry 1 is moved to the device where the liquid needs to be transferred, and the aspiration needle 9 is aligned with the designated discharge position, and then the output shaft of the electric push rod 13 is started to extend, and the aspirated liquid can be discharged into the designated container, thus completing the pipetting work;

[0032] In subsequent use, whenever liquid is sucked, in order to ensure that the amount of liquid sucked meets the actual requirements, the adjusting screw 17 can be turned before sucking, and the positioning plate 18 can rise with the pointing piece 19. At this time, observe the pointing position between the pointing piece 19 and the scale line on the corresponding limit column until the positioning plate 18 is adjusted to the specified height. Afterwards, when the electric push rod 13 extracts liquid, when the mounting frame 12 rises to contact with the positioning plate 18, the suction is stopped. At this time, it means that a certain amount of liquid to be transferred has been extracted from the liquid suction barrel 8.

[0033] Compared with related technologies, the pipetting mechanism of the protein blotting instrument provided by the present invention has the following beneficial effects:

[0034] The present invention provides a liquid transfer mechanism for a protein blotting instrument. By providing a plurality of liquid suction barrels 8, liquid in a plurality of test tubes in a row can be transferred, thereby greatly improving work efficiency. Moreover, when transferring liquid, the content of the body fluid that can be absorbed can be adjusted by adjusting the position of the positioning plate 18, thereby improving work efficiency while ensuring the accurate content of the transferred liquid.

[0035] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the description and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A pipetting mechanism for a protein blotting instrument, comprising an outer gantry and an inner gantry, wherein the inner gantry is located inside the outer gantry, and is characterized in that: The top of the plurality of pull rods is fixedly installed with a plurality of slide seats, and the plurality of slide seats are respectively fixedly connected to the plurality of slide seats, and the plurality of slide seats are threadedly installed with a second fastening screw. The ends of the plurality of second fastening screws are in conflict with the support slide.

2. The liquid transfer mechanism of the protein blotting instrument according to claim 1, characterized in that: Vertical sliding columns are fixedly installed on the inner walls on both sides of the outer gantry, and the top ends of the two vertical sliding columns are fixedly connected to the top inner wall of the outer gantry. Sliding arms are slidably installed on the two vertical sliding columns, and the sides of the two sliding arms close to each other are respectively fixedly connected to the outer walls on both sides of the inner gantry, and the two sliding arms are threadedly installed with first fastening screws, and the ends of the two first fastening screws respectively conflict with the two vertical sliding columns.

3. The liquid transfer mechanism of the protein blotting instrument according to claim 1, characterized in that: Two cross arms are fixedly installed in the inner gantry, and connecting blocks are slidably installed on the two cross arms. The corresponding two connecting blocks are fixedly connected to the corresponding liquid suction buckets.

4. The liquid transfer mechanism of the protein blotting instrument according to claim 1, characterized in that: An adjusting screw is threadedly installed on the top of the inner gantry, and a positioning plate is rotatably installed on the bottom end of the adjusting screw. Two limit columns are slidably installed on the top of the inner gantry, and the bottom ends of the two limit columns are fixedly connected to the mounting frame. One of the limit columns is engraved with scale lines, and a pointing piece is fixedly installed on the side of the positioning plate close to the limit column, and the pointing piece is adapted to the scale lines.

5. The liquid transfer mechanism of the protein blotting instrument according to claim 4, characterized in that: A movable opening is provided on the top of the outer gantry, and the electric push rod and the adjusting screw both pass through the movable opening.

6. The liquid transfer mechanism of the protein blotting instrument according to claim 1, characterized in that: A scale located above the slide is fixedly installed in the installation frame, and a plurality of guide headers are fixedly installed on the tops of the slides, and the plurality of guide headers are adapted to the scale.

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