Improved trace nucleic acid preparation column

Abstract

The utility model relates to the technical field of nucleic acid preparation columns, and discloses an improved trace nucleic acid preparation column, which is characterized in that a necking part is arranged at the joint of a cylinder part and a liquid guide communication part, and the liquid guide communication part is connected and mounted by using the necking part, so that the cross sectional area of a membrane body can be indirectly reduced; the annular supporting table and the three-legged support are arranged on the lower portion of the inner side of the liquid guiding communication part, the annular supporting table is matched with the three-legged support to support the edge and the lower portion of the adsorption film body, the flatness of the film body can be kept while the installation stability of the film body is guaranteed, the annular supporting table has the small necking diameter, and therefore the film body can be stably installed. Through work cooperation of the annular supporting table and the liquid guiding communication part, the flowability of a solution passing through a membrane can be remarkably improved, the collection outer pipe is installed outside the purification column body, and the communication edge groove is formed in the inner edge side of the upper portion of the collection outer pipe, so that the cavity closing effect is not caused even if the purification column body is installed in a drawing and inserting mode in the sealing cover closed state, and the purification effect is improved. And the purification column body is more convenient to pull, insert and mount.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid preparation columns, in particular to an improved micro-nucleic acid preparation column. Background Art

[0002] Nucleic acids are carriers of genetic information, the most important bioinformation molecules, and the objects and tools of many life science studies. The study of nucleic acids in these materials is inseparable from the process of extraction, separation and purification of the nucleic acids therein. Nucleic acid preparation columns are required in the operation of nucleic acid extraction and purification. In order to achieve nucleic acid purification, a nucleic acid adsorption membrane is set inside the nucleic acid preparation column. The micro-sized nucleic acid adsorption membrane can minimize the volume of the subsequent solution used to elute the nucleic acid from the membrane. Generally, the amount of nucleic acid to be eluted is relatively small. The smaller the elution volume, the higher the concentration of the eluted nucleic acid. In order to increase the nucleic acid concentration, the volume of the adsorption membrane needs to be minimized. Reducing the volume can be considered by reducing the number of membrane layers (or thickness), but there is a minimum number of layers. Too few layers are unreliable, and generally not less than 2 layers of membrane. Another way to reduce the volume is to reduce the diameter of the column and reduce the cross-sectional area of ​​the membrane.

[0003] In order to reduce the cross-sectional area of ​​the adsorption membrane, the lower part of the existing column is mostly set with a constricted structure. The constriction of the lower part of the existing nucleic acid preparation column is mostly set with a stepped structure and the width of the stepped surface is relatively large. Although the cross-sectional area of ​​the membrane can be reduced, the lower part of the membrane is blocked by the constricted stepped surface, which will hinder the fluidity of the solution through the membrane. In addition, when the column is installed and connected to the outer tube in a closed state, the chamber is closed, and the operation is very laborious due to the sealing effect of the inner cavity during the plugging and pulling separation operations. The pulling and pulling separation force can easily cause the column to fall out of the hand or the solution to spill. Therefore, an improved trace nucleic acid preparation column is proposed. Utility Model Content

[0004] The purpose of the present invention is to provide an improved micro-nucleic acid preparation column to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned object, the present invention provides the following technical solution: an improved micro-nucleic acid preparation column, comprising a purification column body and a collection outer tube, wherein a cylindrical portion is provided in the middle of the purification column body, a cover mounting portion is provided on the upper portion of the cylindrical portion, a sealing cap is provided on the upper portion of the cover mounting portion, a liquid guide connecting portion is provided at the lower portion of the cylindrical portion, a necking portion is provided at the connection between the cylindrical portion and the liquid guide connecting portion, an annular support and a tripod bracket are provided on the inner lower portion of the liquid guide connecting portion, and a mounting pressure ring is installed inside the liquid guide connecting portion;

[0006] The outer collecting tube is installed on the outside of the purification column, and a communicating side groove is provided on the inner side of the upper opening of the outer collecting tube.

[0007] Preferably, the cylindrical portion is arranged in a cylindrical structure.

[0008] Preferably, the necked portion is a transition section between the cylindrical portion and the liquid-conducting connecting portion, and the liquid-conducting connecting portion is fixedly mounted on the lower portion of the cylindrical portion through the necked portion. The inner diameter of the liquid-conducting connecting portion is 5.0-6.0 mm.

[0009] Preferably, the annular support is arranged on the inner lower opening side of the liquid-conducting connecting portion, and the radial width of the annular support is 0.4-0.5 mm.

[0010] Preferably, the tripod is fixedly mounted on the lower opening side of the liquid-conducting connecting part, and the inner supporting side of the tripod is provided with supporting legs, the number of the supporting legs is three, and the mounting pressure ring is mounted on the inner lower part of the liquid-conducting connecting part through an interlocking support.

[0011] Preferably, the cover mounting portion is fixed to the upper portion of the cylindrical portion, and the sealing cover is mounted on the side of the cover mounting portion via a flexible side seat. A liquid injection port is provided on the upper portion of the cover mounting portion, and the sealing cover is embedded and mounted on the inner side of the liquid injection port when closed.

[0012] Preferably, the above-mentioned collecting outer tube is installed on the outside of the purification column from the bottom, a liquid collecting chamber is provided in the middle of the collecting outer tube, the communicating side groove is provided on the upper opening side of the liquid collecting chamber, an annular support is provided on the upper part of the collecting outer tube, and a communicating notch is provided at the intersection of the annular support and the communicating side groove.

[0013] Compared with the prior art, the above technical solution adopted by the present invention has the following technical effects:

[0014] The nucleic acid preparation column adopts a necking structure, with a necking portion provided at the lower part of the purification column, which is used to connect and install the liquid-conducting connecting portion, thereby indirectly reducing the cross-sectional area of ​​the membrane body, and an annular support is provided on the inner side of the lower part of the liquid-conducting connecting portion, which is used in conjunction with a tripod to respectively support the edge and the lower part of the adsorption membrane body, thereby ensuring the stability of the membrane installation while maintaining the flatness of the membrane body, and the annular support has a smaller necking diameter, and through the cooperation of the annular support and the liquid-conducting connecting portion, the structure of the supporting part at the bottom of the membrane body can be improved while further reducing the membrane area, reducing the obstruction of the support structure to the lower part of the membrane body, and significantly improving the fluidity of the solution through the membrane, and a connecting edge groove is provided on the upper inner side of the collecting outer tube, so that even if the purification column is inserted and installed in the closed state of the cover, it will not cause a closed cavity effect, making the insertion and removal of the purification column more convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0016] Figure 1 This is a schematic diagram of the overall installation three-dimensional structure of the utility model;

[0017] Figure 2 This is a schematic diagram of the overall installation and splitting structure of the utility model;

[0018] Figure 3 For the utility model Figure 2 Schematic diagram of the structure of the middle A area;

[0019] Figure 4 This is a schematic diagram of the upper structure of the purification column of the present utility model;

[0020] Figure 5 This is a schematic diagram of the lower structure of the purification column of the utility model;

[0021] Figure 6 This is a schematic diagram of the cross-sectional structure of the middle part of the purification column of the present utility model.

[0022] Explanation of the accompanying symbols: 1. Purification column; 2. Collection outer tube; 3. Sealing cap; 4. Cover body mounting portion; 5. Liquid guide connecting portion; 6. Narrowing portion; 7. Annular support; 8. Tripod; 9. Mounting pressure ring; 10. Liquid injection port; 11. Connecting side groove; 12. Annular support. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0024] It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the conditions under which this application can be implemented. Therefore, they have no substantive technical significance. Any structural modification, change in proportional relationship or adjustment of size should still fall within the scope of the technical content disclosed in this application without affecting the efficacy and purpose that can be achieved by this application.

[0025] Example

[0026] See also Figure 1-6 The utility model provides a technical solution: an improved micro-nucleic acid preparation column, comprising a purification column 1 and a collection outer tube 2, the purification column 1 is used for the storage and separation and purification of nucleic acid mixed solutions, as shown in the attached Figure 4 As shown, a cylindrical portion is provided in the middle of the purification column 1, and the cylindrical portion is a cylindrical structure, which serves as the main storage structure for the nucleic acid mixed solution. In order to facilitate the filling of the solution, a cover mounting portion 4 is provided on the upper part of the cylindrical portion, and the cover mounting portion 4 is fixed to the upper part of the cylindrical portion. In order to facilitate the insertion of the solution, a liquid injection port 10 is provided on the upper part of the cover mounting portion 4. In order to facilitate the sealing of the liquid injection port 10, a sealing cover 3 is provided on the upper part of the cover mounting portion 4. The sealing cover 3 is installed on the side of the cover mounting portion 4 through a flexible side seat. When closed, the sealing cover 3 is embedded in the inner side of the liquid injection port 10 to prevent the solution from spilling out.

[0027] In order to facilitate the downward movement of the solution, Figure 4 As shown, a liquid conducting connecting portion 5 is provided at the lower part of the column part. In order to reduce the cross-sectional area of ​​the adsorption membrane body and increase the concentration of nucleic acid, a necking portion 6 is provided at the connection between the column part and the liquid conducting connecting portion 5. The necking portion 6 is a transition section between the column part and the liquid conducting connecting portion 5. The liquid conducting connecting portion 5 is fixedly installed at the lower part of the column part through the necking portion 6. The inner diameter of the liquid conducting connecting portion 5 is 5.0 mm. The adsorption membrane body is installed at the lower part of the liquid conducting connecting portion 5. Placing the membrane body at the bottom end of the liquid conducting connecting portion 5 can make full use of the volume of the column and expand the column volume as much as possible. At the same time, placing the membrane body at the bottom end allows the solution to be directly thrown out from here, and there is no dead angle during centrifugation.

[0028] As attached Figure 6 As shown, an annular support 7 and a tripod 8 are provided at the inner lower part of the liquid-conducting connecting part 5. The annular support 7 is provided at the inner lower opening side of the liquid-conducting connecting part 5. The radial width of the annular support 7 is 0.4 mm. Excluding the 0.4 mm annular support 7 around the edge, there is still about 4.2 mm of space in the inner diameter of the lower opening of the annular support 7. The inner circle side of the annular support 7 is provided with an arc chamfer, so that the bottom of the adsorption membrane body can be directly exposed, and the solution can be filtered through the unobstructed part under the membrane body, which can significantly improve the fluidity of the solution through the membrane.

[0029] In order to support the lower part of the adsorption membrane, Figure 6As shown, the tripod 8 is fixedly installed on the lower opening side of the liquid-conducting connecting part 5. The tripod 8 can support the entire membrane, and the annular support 7 can well support the edge of the membrane to keep the membrane flat. The triangular support 8 is composed of three legs. The conventional six or eight legs are not used here, mainly because of the volume. So many legs cannot be arranged. In addition, the annular support 7 cooperates with the triangular support 8 to reliably support the membrane, and no more legs are needed.

[0030] In order to limit the installation of the membrane, as shown in the attached Figure 6 As shown, a mounting pressure ring 9 is installed inside the liquid-conducting connecting part 5, and the mounting pressure ring 9 is mounted on the inner lower part of the liquid-conducting connecting part 5 in an interlocking manner. In conjunction with the mounting pressure ring 9, the nucleic acid adsorption membrane can be stably and reliably fixed in the required position. Through the cooperation of the annular support 7 and the liquid-conducting connecting part 5, the structure of the supporting part at the bottom of the membrane body can be improved while further reducing the membrane area, reducing the obstruction of the support structure to the lower part of the membrane body, and significantly improving the fluidity of the solution through the membrane.

[0031] The collecting outer tube 2 is installed on the outside of the purification column 1 and is used to collect the circulating solution. Figure 2 As shown, the collecting outer tube 2 is installed on the outside of the purification column 1 from the bottom. In order to facilitate the collection of the solution, a liquid collection cavity is provided in the middle of the collecting outer tube 2. In order to facilitate the plugging and unplugging of the purification column 1, as shown in the attached figure, Figure 3 As shown, a communicating side groove 11 is provided on the inner side of the upper opening of the collecting outer tube 2. The communicating side groove 11 is provided on the upper opening side of the liquid collecting chamber. Even if the purification column 1 is inserted and installed when the cover 3 is closed, it will not cause a closed cavity effect, making the insertion and installation of the purification column 1 more convenient. In order to provide a communicating gap, an annular support 12 is provided on the upper part of the collecting outer tube 2, and a communicating notch is provided at the intersection of the annular support 12 and the communicating side groove 11.

[0032] The working principle or structural principle is that when in use, the adsorption membrane body is installed into the inner lower part of the purification column 1. After installation, the side of the adsorption membrane body is supported and installed by the annular support 7, and the tripod 8 supports and supports the lower part of the adsorption membrane body. Then, the installation pressure ring 9 is installed into the interior of the purification column 1, and the upper part of the adsorption membrane body is installed and limited by the installation pressure ring 9. After completion, the nucleic acid solution and reagent are loaded, and the collection outer tube 2 is installed on the outside of the purification column 1. When the collection outer tube 2 is installed, even if the cover 3 is closed, during the plug-in installation process, the connection effect of the connecting edge groove 11 can avoid the formation of a closed inner cavity, making the installation of the collection outer tube 2 more convenient and labor-saving. When performing the purification operation, the device is placed in a centrifugal device. Under the action of centrifugal force, the solution in the purification column 1 flows downward through the adsorption membrane body into the collection outer tube 2. During the downward flow, it will not be excessively blocked by the shrinking terrace structure, which can significantly improve the fluidity of the solution through the membrane.

[0033] The closure 6 is provided on the bottom of the closure 6 so that the closure 6 can be tightened to the bottom of the closure 6. The closure 6 is provided on the bottom of the closure 6 so that the closure 6 can be tightened to the bottom of the closure 6. The closure 6 is provided on the bottom of the closure 6 so that the closure 6 can be tightened to the bottom of the closure 6. The closure 6 is provided on the bottom of the closure 6 so that the closure 6 can be tightened to the bottom of the closure 6.

[0034] Those skilled in the art will appreciate that various combinations and / or combinations of features described in the various embodiments and / or claims of the present invention may be employed, even if such combinations and / or combinations are not explicitly described in the present invention. In particular, various combinations and / or combinations of features described in the various embodiments and / or claims of the present invention may be employed without departing from the spirit and teachings of the present invention. All such combinations and / or combinations fall within the scope of the present invention.

Claims

1. An improved micro-nucleic acid preparation column, comprising a purification column (1) and a collection outer tube (2), characterized in that: The middle part of the purification column (1) is provided with a cylindrical portion, the upper part of the cylindrical portion is provided with a cover body mounting portion (4), the upper part of the cover body mounting portion (4) is provided with a sealing cover (3), the lower part of the cylindrical portion is provided with a liquid conducting connecting portion (5), the connection between the cylindrical portion and the liquid conducting connecting portion (5) is provided with a necking portion (6), the inner lower part of the liquid conducting connecting portion (5) is provided with an annular support (7) and a tripod bracket (8), and the interior of the liquid conducting connecting portion (5) is provided with a mounting pressure ring (9); The collecting outer tube (2) is installed outside the purification column (1), and a communicating side groove (11) is provided on the inner side of the upper opening of the collecting outer tube (2).

2. The improved column for preparing micro-amount nucleic acid according to claim 1, characterized in that: The cylindrical portion is arranged in a cylindrical structure.

3. The improved column for preparing micro-amount nucleic acid according to claim 2, characterized in that: The necking portion (6) is a transition section between the cylindrical portion and the liquid-conducting communication portion (5). The liquid-conducting communication portion (5) is fixedly mounted on the lower portion of the cylindrical portion through the necking portion (6). The inner diameter of the liquid-conducting communication portion (5) is 5.0-6.0 mm.

4. The improved column for preparing micro-amount nucleic acid according to claim 3, characterized in that: The annular support platform (7) is arranged on the inner lower opening side of the liquid-conducting communication portion (5), and the radial width of the annular support platform (7) is 0.4-0.5 mm.

5. The improved column for preparing micro-amount nucleic acid according to claim 4, characterized in that: The tripod bracket (8) is fixedly mounted on the lower opening side of the liquid-conducting communication part (5), and the inner supporting side of the tripod bracket (8) is provided with supporting legs, the number of the supporting legs being three, and the mounting pressure ring (9) is mounted in a chimeric support manner on the inner lower part of the liquid-conducting communication part (5).

6. The improved column for preparing micro-amount nucleic acid according to claim 1, characterized in that: The cover body mounting portion (4) is fixed to the upper part of the cylindrical portion, and the sealing cover (3) is mounted on the side of the cover body mounting portion (4) through a flexible side seat connection. A liquid injection port (10) is provided on the upper part of the cover body mounting portion (4). When closed and installed, the sealing cover (3) is embedded and mounted on the inner side of the liquid injection port (10).

7. The improved column for preparing micro-amount nucleic acid according to claim 1, characterized in that: The collecting outer tube (2) is installed on the outside of the purification column (1) from the bottom, a liquid collecting cavity is provided in the middle of the collecting outer tube (2), the communicating side groove (11) is provided on the upper opening side of the liquid collecting cavity, an annular support (12) is provided on the upper part of the collecting outer tube (2), and a communicating notch is provided at the intersection of the annular support (12) and the communicating side groove (11).

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