Intelligent microplate reader with molecular diagnosis function
By integrating the molecular diagnostic module and cuvette detection module in the microplate reader, the problem of single function of the enzyme-linked immunoassay device is solved, and absorbance measurement and molecular diagnosis are achieved simultaneously, improving experimental efficiency and accuracy.
Patent Information
- Application Number
- CN202422242562.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-12
- Publication Date
- 2025-08-26
- Estimated Expiration
- 2034-09-12
AI Technical Summary
The existing enzyme-linked immunoassay device has a single function and cannot meet the needs of molecular diagnosis.
An intelligent microplate reader was designed, integrating molecular diagnostic functions, including molecular diagnostic module, cuvette detection module and voice control functions, supporting absorbance measurement and molecular diagnosis, and has dual-channel fluorescence detection and full-wavelength cuvette functions.
It realizes simultaneous absorption measurement and molecular diagnosis on the same device, improves experimental efficiency and versatility of the instrument, reduces operation inconvenience, and improves experimental accuracy.
Smart Images
Figure CN223268648U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of intelligent enzyme markers, in particular to an intelligent enzyme marker with a molecular diagnosis function. Background Art
[0002] An enzyme-linked immunosorbent assay (ELISA) reader is a specialized instrument for enzyme-linked immunosorbent assays (ELISAs), widely used in fields such as biochemistry and molecular biology. However, existing ELISA analyzers are primarily limited to detecting ELISA reactions and cannot meet the needs of molecular diagnostics.
[0003] The existing enzyme-linked immunosorbent analyzers are relatively simple in function. For certain specific application scenarios and multiple application requirements, the existing equipment is difficult to meet the needs; therefore, to address the above problems, an ultrafiltration membrane stack using a new aeration method is proposed. Utility Model Content
[0004] In order to make up for the deficiencies of the prior art, the utility model proposes an intelligent enzyme marker with molecular diagnosis function.
[0005] The technical solution adopted by the utility model to solve its technical problems is: an intelligent enzyme marker with molecular diagnosis function, including an intelligent enzyme marker, the right side of the plate body of the intelligent enzyme marker is fixedly connected with a support column 2, the rear side of the support column 2 is provided with a support column 3, the left side of the plate body of the intelligent enzyme marker is fixedly connected with a support column 1, the lower part of the plate body of the intelligent enzyme marker is fixedly connected with a side plate, the bottom of the right side of the side plate is fixedly connected with a linear motor, a position detector is provided at the output shaft of the linear motor, the rear side of the side plate is fixedly connected with a baffle 1, the middle part of the side plate is fixedly connected with a linear motor 1, the left side of the side plate is fixedly connected with a linear motor 2, the end of the linear motor 2 is meshedly connected with a transmission rack, the left side of the moving arm connected to the transmission rack is provided with a cuvette detection module, the right side of the moving arm is provided with a molecular diagnosis module, the rear side of the moving arm is provided with a baffle 2, and the moving arm is plugged with The control panel is connected to the control panel, and the control panel has two guide rails, which are used to guide the display screen of the instrument. The control panel has two guide rails, which are used to control the display screen of the instrument. The control panel has two guide rails, which are used to control the display screen of the instrument. The control panel has two guide rails, which are used to control the display screen of the instrument.
[0006] Preferably, the molecular diagnosis module includes: a molecular diagnosis base, a nucleic acid amplification unit 1, a nucleic acid amplification unit 2, an optical path acquisition and processing unit 1, an excitation light unit, a molecular diagnosis base, and an optical path acquisition and processing unit 2. The frame of the nucleic acid amplification unit 1 is fixedly connected to the nucleic acid amplification unit 2. The nucleic acid amplification unit 1 is plugged into the cuvette detection module, the optical path acquisition and processing unit 1 is plugged into the front side of the movable arm, the molecular diagnosis base is plugged into one side of the movable arm, the excitation light unit is plugged into the end of the molecular diagnosis base, the nucleic acid amplification unit 3 is plugged into the lower part of the movable arm, and the optical path acquisition and processing unit 2 is plugged into the rear side of the movable arm.
[0007] Preferably, the cuvette detection module comprises: a cuvette base and an incubation unit, the cuvette base is plugged into the side of the movable arm, and the end of the cuvette base is plugged into one side of the incubation unit.
[0008] The utility model is beneficial in that:
[0009] (1) Increase the cross-domain function of the instrument: users do not need to purchase other molecular diagnostic equipment and enzyme-linked immunosorbent analyzers to operate experiments across devices. They can perform absorbance measurement and molecular diagnosis at the same time, which improves the practicality and versatility of the instrument. One device can directly complete two or more experimental operations, greatly improving experimental efficiency.
[0010] (2) Adding an enzyme-linked immunosorbent analyzer with voice control function: Since there are inconveniences for experimenters during the operation of the instrument, the voice control function provides experimenters with operational convenience, reduces the inconvenience of the experiment, improves the experimenters' operating efficiency and ensures the accuracy of the experiment.
[0011] (3) Adding dual-channel fluorescence detection function: When the temperature control function is not used in the added molecular diagnosis module, this area is a single-hole dual-channel fluorometer.
[0012] (5) Add a molecular diagnostic module and combine it with the full-wavelength cuvette function module: The optical path signal of the cuvette module and the optical signal of the molecular diagnostic module are collected and converted on a single circuit for digital-to-analog conversion and sent to the controller for processing. The cuvette module has a full-wavelength light source and a 37°C incubation function. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.
[0014] Figure 1 It is a schematic diagram of the right side three-dimensional structure of the utility model;
[0015] Figure 2 It is a schematic diagram of the left three-dimensional structure of the utility model;
[0016] Figure 3 It is a schematic diagram of the local structure of the utility model.
[0017] In the picture:
[0018] 1. Grating monochromator; 2. Light input fiber 1; 3. Light input fiber 2; 4. Position detector; 5. Linear motor; 6. ELISA plate holder; 7. Side panel; 8. Support column 1; 9. Support column 2; 10. Support column 3; 11. Drive rack; 12. Screw motor; 13. Screen support column; 14. Absorbance detector; 15. Reference detector; 16. Molecular diagnostic module; 17. Cuvette detection module; 18. Controller; 19. Display; 20. Temperature detector Device; 21. Baffle 1; 22. Baffle 2; 23. Baffle 3; 24. Baffle 4; 25. Linear motor 1; 26. Linear motor 2; 27. Scanning frame; 162. Nucleic acid amplification unit 1; 163. Nucleic acid amplification unit 2; 166. Nucleic acid amplification unit 3; 161. Molecular diagnostic base; 165. Excitation light unit; 164. Optical path acquisition and processing unit 1; 167. Optical path acquisition and processing unit 2; 172. Incubation unit; 171. Cuvette base. DETAILED DESCRIPTION
[0019] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.
[0020] The following is combined with Figure 1-3 To further explain this application,
[0021] The embodiment of the present application discloses an intelligent enzyme labeling instrument with a molecular diagnosis function, including an intelligent enzyme labeling instrument, wherein a support column 2 9 is fixedly connected to the right side of the plate body of the intelligent enzyme labeling instrument, a support column 3 10 is provided on the rear side of the support column 2 9, a support column 1 8 is fixedly connected to the left side of the plate body of the intelligent enzyme labeling instrument, a side plate 7 is fixedly connected to the lower part of the plate body of the intelligent enzyme labeling instrument, a linear motor 5 is fixedly connected to the bottom of the right side of the side plate 7, a position detector 4 is provided at the output shaft of the linear motor 5, a baffle 1 21 is fixedly connected to the rear side of the side plate 7, a linear motor 1 25 is fixedly connected to the middle part of the side plate 7, a linear motor 26 is fixedly connected to the left side of the side plate 7, the end of the linear motor 26 is meshedly connected with a transmission rack 11, a cuvette detection module 17 is provided on the left side of the moving arm connected to the transmission rack 11, a molecular diagnosis module 16 is provided on the right side of the moving arm, a baffle 2 22 is provided on the rear side of the moving arm, and a temperature detector 20 is plugged into the moving arm. The left end of the plate body of the microplate reader is movably connected to the microplate holder 6, and a plurality of controllers 18 are provided on the side of the plate body of the intelligent microplate reader. The upper part of the plate body of the intelligent microplate reader is fixedly connected to the screw motor 12, and the middle part of the screw motor 12 is provided with a baffle 24. The rod body of the screw motor 12 is movably connected to the screen support column 13, and the end of the screen support column 13 is fixedly connected to the display 19. The upper part of the plate body of the intelligent microplate reader is fixedly connected to the grating monochromator 1, and the lower part of the plate body of the intelligent microplate reader is fixedly connected to the grating monochromator 1. The slide rail is slidably connected to a scanning frame 27, the upper end of the scanning frame 27 is fixedly connected to an absorbance detector 14, the lower end of the scanning frame 27 is fixedly connected to a reference detector 15, and a baffle 3 23 is provided at the lower part of the scanning frame 27. The socket of the grating monochromator 1 is plugged with an input optical fiber 1 2, and the end of the input optical fiber 1 2 is plugged into the socket of the scanning frame 27. The socket of the grating monochromator 1 is plugged with an input optical fiber 2 3, and the end of the input optical fiber 2 3 is plugged into the socket of the moving arm;
[0022] The molecular diagnosis module 16 includes: a molecular diagnosis base 161, a nucleic acid amplification unit 1 162, a nucleic acid amplification unit 2 163, an optical path acquisition and processing unit 1 164, an excitation light unit 165, the molecular diagnosis base 161, and an optical path acquisition and processing unit 2 167. The frame of the nucleic acid amplification unit 1 162 is fixedly connected to the nucleic acid amplification unit 2 163. The nucleic acid amplification unit 1 162 is plugged into the cuvette detection module 17. The optical path acquisition and processing unit 1 164 is plugged into the front side of the movable arm. The molecular diagnosis base 161 is plugged into one side of the movable arm. The excitation light unit 165 is plugged into the end of the molecular diagnosis base 161. The nucleic acid amplification unit 3 166 is plugged into the lower part of the movable arm. The optical path acquisition and processing unit 2 167 is plugged into the rear side of the movable arm.
[0023] The cuvette detection module 17 includes a cuvette base 171 and an incubation unit 172 . The cuvette base 171 is plugged into the side of the moving arm, and an end of the cuvette base 171 is plugged into one side of the incubation unit 172 .
[0024] Working principle: the grating monochromator is used to emit light of full wavelength; the light input optical fiber 2 is used to guide the light from the grating monochromator 1 to the detection position; the ELISA plate rack 6 is used to carry the test reagent; the reference detector 15 is used to detect the accuracy of the original light source as a reference; the absorbance detector 14 is used to detect the absorbance intensity; the linear motor 5 is used to move each moving part; the screen support column 13 is used to support the display 19 for angle adjustment; the molecular diagnosis module 16 is used to perform molecular diagnosis on the sample, including nucleic acid amplification and detection functions; the cuvette detection module 17 is a detection mechanism for light absorption at a specific wavelength; the controller 18 is used to control each enabling mechanism, and the display 19 is used to input operation instructions for each function and display data detection results; the temperature detector 15 is used for real-time temperature acquisition of the absorbance functional area; the baffle 21 is used to sense the position detector to obtain the detection position of the sample addition area.
[0025] During the specific implementation of the molecular diagnosis function, the test tube of the detection reagent needs to be placed in the nucleic acid amplification unit 1 162, the nucleic acid amplification unit 2 163, and the nucleic acid amplification unit 3 166. The display 19 is set to set the operating instructions so that it is heated to the set temperature around the adapter. There is a separate temperature sensor at the bottom of the adapter to collect the temperature in real time. When the preset temperature is reached, the excitation unit will emit a specific light, which will pass through the optical filtering unit and enter the test tube of the detection reagent. Then, it will excite light of another wavelength in the direction of the optical path and further enter the optical path convergence filtering unit. After being collected by the optical path collection and processing unit, it will be transmitted to the optical path collection and processing unit for processing and then uploaded to the control The result is processed by the controller and finally uploaded to the display 19 for display to the user. After N cycles, the reagent is continuously amplified and the fluorescence intensity is continuously increased, forming an amplification curve relationship with the number of cycles and the relative fluorescence intensity, so as to determine whether it is negative or positive, thereby achieving a diagnostic purpose; when dual-channel detection is required, just open another 90-degree optical path channel to excite the light unit to emit specific light, which is injected into the detection reagent tube through the optical filtering unit, and then excites another wavelength of light in a 90-degree optical path direction and further shoots into the optical path convergence filtering unit. The collection needs to be completed by the optical path collection and processing unit, and then uploaded to the controller 18 for processing and finally uploaded to the display for display to the user.
[0026] During the specific implementation of the cuvette detection module 17 function, first, place an empty 10mm standard cuvette into the cuvette base, click the display to perform a blank control, and then take out the empty 10mm standard cuvette. Further, it is necessary to add the test agent into the 10mm standard cuvette, and then place the cuvette into the cuvette base. If an incubation operation is required, first set the operation instruction on the setting display 19, and it needs to be incubated at 37 degrees Celsius. Further, the controller 18 will control the incubation unit to feedback the real-time temperature through a separate temperature sensor. If the preset temperature is not reached, the heating membrane of the incubation unit 172 on both sides of the cuvette base 171 will work until the temperature is reached; after the current operation is completed or the next operation is directly performed, then click the display 19 to click on sample detection, the controller 18 will control the light excitation unit to emit light of a specific wavelength, pass through the light path convergence unit and the aperture, and then pass through the standard cuvette filled with the reagent, and further pass through the light convergence unit and the light filtering unit. Finally, it is detected by the light path acquisition and processing unit 164, processed, sent to the controller 18 for processing, and finally uploaded to the display 19 for display to the user.
[0027] The enzyme-linked immunosorbent analyzer (ELISA) of the present invention incorporates molecular diagnostic capabilities, enabling simultaneous absorbance measurement and molecular diagnosis, enhancing the instrument's practicality and versatility. The molecular diagnostic module utilizes an integrated design for nucleic acid amplification and detection, enabling simple and rapid operation and improving detection efficiency. A controller processes and analyzes test data and displays the results on a display screen for easy user review and operation.
[0028] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and improvements may be made to the present invention without departing from the spirit and scope of the present invention, and such changes and improvements fall within the scope of the present invention as claimed.
Claims
1. An intelligent microplate reader with molecular diagnostic function, comprising an intelligent microplate reader, characterized in that: The right side of the plate body of the intelligent microplate reader is fixedly connected to a support column 2 (9), the rear side of the support column 2 (9) is provided with a support column 3 (10), the left side of the plate body of the intelligent microplate reader is fixedly connected to a support column 1 (8), the lower part of the plate body of the intelligent microplate reader is fixedly connected to a side plate (7), the bottom of the right side of the side plate (7) is fixedly connected to a linear motor (5), a position detector (4) is provided at the output shaft of the linear motor (5), the rear side of the side plate (7) is fixedly connected to a baffle 1 (21), the middle part of the side plate (7) is fixedly connected to the side plate (7). The side plate (7) is fixedly connected to a linear motor 1 (25), the left side of the side plate (7) is fixedly connected to a linear motor 2 (26), the end of the linear motor 2 (26) is meshedly connected to a transmission rack (11), the left side of the mobile arm connected to the transmission rack (11) is provided with a cuvette detection module (17), the right side of the mobile arm is provided with a molecular diagnosis module (16), the rear side of the mobile arm is provided with a baffle 2 (22), the mobile arm is plugged with a temperature detector (20), the left end of the plate body of the intelligent microplate reader is movably connected to the microplate holder (6 ), a plurality of controllers (18) are provided on the side of the plate body of the intelligent microplate reader, a screw motor (12) is fixedly connected to the upper part of the plate body of the intelligent microplate reader, a baffle (24) is provided in the middle of the screw motor (12), the rod body of the screw motor (12) is movably connected to the screen support column (13), the end of the screen support column (13) is fixedly connected to the display (19), the upper part of the plate body of the intelligent microplate reader is fixedly connected to the grating monochromator (1), and the slide rail at the lower part of the plate body of the intelligent microplate reader is slidably connected to the scanning frame (2 7), the upper end of the scanning frame (27) is fixedly connected to an absorbance detector (14), the lower end of the scanning frame (27) is fixedly connected to a reference detector (15), the lower part of the scanning frame (27) is provided with a baffle three (23), the socket of the grating monochromator (1) is plugged with a light-incoming optical fiber one (2), the end of the light-incoming optical fiber one (2) is plugged into the socket of the scanning frame (27), the socket of the grating monochromator (1) is plugged with a light-incoming optical fiber two (3), the end of the light-incoming optical fiber two (3) is plugged into the socket of the movable arm.
2. The intelligent microplate reader with molecular diagnostic function according to claim 1, characterized in that: The molecular diagnosis module (16) includes: a molecular diagnosis base (161), a nucleic acid amplification unit 1 (162), a nucleic acid amplification unit 2 (163), an optical path acquisition and processing unit 1 (164), an excitation light unit (165), a nucleic acid amplification unit 3 (166), and an optical path acquisition and processing unit 2 (167). The frame of the nucleic acid amplification unit 1 (162) is fixedly connected to the nucleic acid amplification unit 2 (163). The nucleic acid amplification unit 1 (162) is plugged into the cuvette detection module (17). The optical path acquisition and processing unit 1 (164) is plugged into the front side of the movable arm. The molecular diagnosis base (161) is plugged into one side of the movable arm. The excitation light unit (165) is plugged into the end of the molecular diagnosis base (161). The nucleic acid amplification unit 3 (166) is plugged into the lower part of the movable arm. The optical path acquisition and processing unit 2 (167) is plugged into the rear side of the movable arm.
3. The intelligent microplate reader with molecular diagnostic function according to claim 1, characterized in that: The cuvette detection module (17) comprises: a cuvette base (171) and an incubation unit (172); the cuvette base (171) is plugged into the side of the movable arm; and the end of the cuvette base (171) is plugged into one side of the incubation unit (172).