Auxiliary device for visual detection card and detection device

Abstract

The utility model provides an auxiliary device for a visual detection card, which comprises a box body used for being jointed with the visual detection card, an accommodating chamber arranged in the box body, a PCR (Polymerase Chain Reaction) tube matched with the accommodating chamber, and a cutting piece arranged in the accommodating chamber and used for cutting the PCR tube, the auxiliary device further comprises a calibration bin and a floating plug, wherein the calibration bin is arranged below the cutting piece and contains calibration liquid, and the floating plug is used for bearing the cutting piece and separating the containing cavity from the calibration bin.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection, in particular to an auxiliary device for a visual detection card and a detection device. Background Art

[0002] In recent years, with the advancement of molecular biology techniques and the development of nucleic acid amplification technology, the detection of pathogens has become faster and more accurate. Recombinase Polymerase Amplification (RPA) technology is a new type of nucleic acid amplification technology that has the potential to replace the traditional polymerase chain reaction (PCR). RPA technology combines the unwinding ability of recombinase and single-stranded DNA-binding protein (SSB) on double-stranded DNA, and the extension ability of DNA polymerase on primers, to achieve rapid amplification of target nucleic acids under constant temperature conditions. Compared with PCR technology, RPA technology has significant advantages such as mild reaction conditions, fast reaction speed, and simple operation. However, RPA technology still faces some challenges, such as the problem of false positive results due to cross-contamination or aerosol contamination during operation.

[0003] Therefore, it is desired in the art to provide an auxiliary device for a visualization detection card to solve the above technical problems. Utility Model Content

[0004] The purpose of the utility model is to propose an auxiliary device for a visual detection card, which can ensure that the amplification product does not come into contact with the outside air during the entire RPA on-site detection process, thereby avoiding the problem of aerosol contamination of the reaction product caused by opening the cover during the amplification and sample addition process.

[0005] According to a first aspect of the present invention, an auxiliary device for a visualization test card is provided, comprising a box body for engaging with the visualization test card, wherein a receiving chamber is provided in the box body.

[0006] a PCR tube adapted to the receiving chamber, and

[0007] a cutting piece disposed in the accommodating chamber and used for cutting the PCR tube,

[0008] The auxiliary device further includes a calibration chamber disposed below the cutting member and containing a calibration liquid, and a float for supporting the cutting member and separating the accommodating chamber from the calibration chamber.

[0009] In one embodiment, the accommodating chambers are provided with two and are arranged at intervals, and the PCR tube consists of a first test tube containing an amplification product and a second test tube configured as an empty tube.

[0010] In one embodiment, the float plug is configured as a cylindrical structure, and an outer peripheral surface of the float plug forms a sealed connection with an inner peripheral surface of the accommodating chamber.

[0011] In one embodiment, the cutting element is configured in the form of a prism and is fixed to the upper end surface of the floating plug.

[0012] The PCR tube is configured to move downward under external force and be cut by the cutting member until the float is pushed downward to allow the accommodating chamber to communicate with the calibration chamber, thereby forming a mixed liquid of the amplified product and the calibration liquid in the calibration chamber.

[0013] In one embodiment, the outer diameter of the PCR tube is not greater than the inner diameter of the containing chamber.

[0014] In one embodiment, a guide cavity is provided in the box body and below the calibration chamber.

[0015] The auxiliary device further includes a floating platform disposed in the guide cavity and used for sealing the calibration chamber.

[0016] In one embodiment, the floating platform includes a sealing portion forming a sealed connection with the guide cavity, and a limiting portion arranged above the sealing portion and used to abut against the PCR tube.

[0017] According to a second aspect of the present invention, a detection device is provided, characterized in that it includes a visual detection card and an auxiliary device as described above that is matched with the visual detection card.

[0018] In one embodiment, the visual detection card includes a first card shell, a second card shell adapted to the first card shell, and a guide groove longitudinally arranged on the second card shell.

[0019] The guide groove is configured to lift up the floating platform so that the mixed liquid flows into the first cartridge through the guide groove.

[0020] In one embodiment, a test strip extending longitudinally and used to receive the mixed liquid is provided in the first housing.

[0021] Compared with the prior art, the advantages of the present invention are:

[0022] The present invention uses an auxiliary device in conjunction with a visual test card, thereby being able to mix the amplification product with the calibration liquid through mechanical movement, and to lift the floating platform through the guide groove so that the mixed liquid formed by the amplification product and the calibration liquid crawls on the test strip, and then the result can be interpreted by the change of the test strip. In addition, during the entire RPA on-site detection process, it is possible to ensure that the amplification product will not come into contact with the outside air, thereby avoiding the problem of aerosol contamination of the reaction product caused by opening the cover during the amplification and sample addition process. In addition, the present device has the function of being easy to carry and operate and easy to observe the experimental results. It can effectively improve the detection efficiency during RPA on-site detection, and further meet the needs of RPA on-site rapid detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The present invention will be described in detail below with reference to the accompanying drawings, in which:

[0024] Figure 1 The structure of the detection device according to the present utility model is schematically shown;

[0025] Figure 2 A partial cross-sectional view of a detection device according to the present invention;

[0026] Figure 3 for Figure 1 A first partial view of an auxiliary device for visualizing a test card;

[0027] Figure 4 for Figure 1 A second partial view of the auxiliary device for visualizing the test card;

[0028] In the drawings, like parts are given like reference numerals, but the drawings are not necessarily drawn to scale. DETAILED DESCRIPTION

[0029] To make the technical solutions and advantages of the present invention more clearly understood, exemplary embodiments of the present invention are further described in detail below with reference to the accompanying drawings. It should be understood that the described embodiments are only a portion of the embodiments of the present invention, and are not exhaustive. Furthermore, the embodiments and features of the embodiments of the present invention may be combined with each other unless there is a conflict.

[0030] The present invention will be further described below with reference to the accompanying drawings.

[0031] Figure 1 The structure of the detection device 200 according to the present invention is schematically shown.

[0032] Figure 3 for Figure 1 A first partial view of the auxiliary device 100 for visualizing the test card.

[0033] Figure 4 for Figure 1 FIG. 1 is a second partial view of the auxiliary device 100 for visualizing the test card.

[0034] like Figure 1 、 3 As shown in Figures 4 and 5, according to a first aspect of the present invention, an auxiliary device 100 for a visual test card primarily comprises a box body 10 and a PCR tube 11. Preferably, the box body 10 is capable of engaging with the visual test card 200 to prevent aerosol contamination that could affect test results. A housing chamber is provided within the box body 10, and the outer circumferential surface of the PCR tube 11 is capable of forming a sealed connection with the inner circumferential surface of the housing chamber, further enhancing the sealing performance of the auxiliary device 100 for a visual test card.

[0035] In the present invention, there are two accommodating chambers arranged at intervals, and the PCR tube 11 is composed of two test tubes, one of which is a sealed consumable material for accommodating the amplification product, namely the first test tube, and the other is an empty tube consumable, namely the second test tube. Preferably, one accommodating chamber corresponds to only one test tube. In this way, the present invention can maintain a stable reaction when the mixed reagents flow into the test strip 204 (described below) in the visual detection card 200.

[0036] In a specific embodiment of the present invention, the inner diameter of the accommodating chamber is about 8 mm, the height of the accommodating chamber is about 17 mm; and the height of the PCR tube 11 is about 22 mm.

[0037] In one embodiment, Figure 3 and 4 As shown, the auxiliary device 100 for visual detection card further comprises a cutting member 12 disposed in the accommodating chamber. Preferably, the cutting member 12 is configured as a blade, which can cut the bottom of the PCR tube 11, thereby releasing the amplification product in the PCR tube 11.

[0038] Preferably, the cutting member 12 is configured as a prismatic metal blade, and the cutting end of the cutting member 12 faces the bottom of the PCR tube 11 to ensure that the cutting action can be completed smoothly after the PCR tube 11 descends.

[0039] Preferably, if Figure 3 and 4 As shown, the auxiliary device 100 for visual inspection of the card further includes a float plug 14 disposed below the cutting member 12. The float plug 14 is fixed to the bottom of the accommodating chamber and is configured as a cylindrical structure. The outer circumference of the float plug 14 can form a sealed connection with the inner circumference of the accommodating chamber, thereby fully sealing the accommodating chamber.

[0040] In the present invention, the float 14 is made of plastic material, so it can isolate the blade from the calibration fluid, further preventing the blade from rusting before working.

[0041] In the present invention, the cutting member 12 is fixedly mounted on the upper end surface of the float 14, so that the float 14 can effectively support the cutting member 12, and the cutting member 12 can also achieve synchronous movement with the float 14, which is conducive to connecting the accommodating chamber with the calibration chamber 13 (introduced below).

[0042] In one embodiment, Figure 3 and 4 As shown, the auxiliary device 100 for a visual test card further includes a calibration chamber 13 disposed below a float 14. Preferably, calibration chamber 13 contains a calibration fluid that can be mixed with the amplification product to help improve the accuracy and authenticity of the test results. Preferably, float 14 not only supports cutting element 12 but also completely isolates cutting element 12 from calibration chamber 13 in the initial state.

[0043] In the present invention, the PCR tube 11 can descend under the action of external force and be cut by the cutting piece 12, and continue to descend until the float 14 is prompted to enter the calibration chamber 13, thereby allowing the accommodating chamber to be connected with the calibration chamber 13, which is conducive to the formation of a mixed liquid between the amplification product and the calibration liquid in the calibration chamber 13, further improving the accuracy and authenticity of the test results.

[0044] Preferably, the outer diameter of the PCR tube 11 is no larger than the inner diameter of the receiving chamber. This ensures that the PCR tube 11 remains centered during insertion into the receiving chamber, facilitating the cutting member 12 to cut through the bottom of the PCR tube 11 and thereby smoothly release the amplified product.

[0045] In one embodiment, Figure 3 and 4 As shown, a guide cavity 15 is provided within the box body 10 and below the calibration chamber 13. Preferably, the auxiliary device 100 for the visual test card further includes a floating platform 20 disposed within the guide cavity 15 and configured to seal the calibration chamber 13. Preferably, during the subsequent engagement process, a guide groove 203 (described below) of the visual test card 200 can push the floating platform 20 into the calibration chamber 13, thereby connecting the calibration chamber 13 with the visual test card 200, as described below.

[0046] In one embodiment, Figure 4As shown, the floating platform 20 includes a sealing portion 21 with a larger diameter and a limiting portion 22 with a smaller diameter. Preferably, the sealing portion 21 forms a sealed connection with the guide cavity 15 and does not completely fill the guide cavity 15, thereby facilitating the entry of the guide groove 203 into the guide cavity 15. Preferably, the limiting portion 22 is positioned above the sealing portion 21, and when the limiting portion 22 axially abuts the PCR tube 11, the maximum upward distance of the floating platform 20 is reached.

[0047] Figure 2 A partial cross-sectional view of a detection device according to the present invention;

[0048] According to the second aspect of the present invention, Figure 1 and 2 As shown, a detection device is provided, characterized in that it includes a visual detection card 200 and an auxiliary device 100 as described above that is matched with the visual detection card 200.

[0049] In one embodiment, Figure 1 and 2 As shown, the visual detection card 200 includes a first card housing 201, a second card housing 202 adapted to the first card housing 201, and a guide slot 203 longitudinally disposed on the second card housing 202. Preferably, the guide cavity 15 of the auxiliary device 100 can be docked with the guide slot 203 of the visual detection card 200 to complete the RPA on-site detection work.

[0050] Preferably, both the first and second cartridges 201 and 202 are enclosed, transparent cartridges, allowing results to be determined without opening. This ensures that the amplified product is protected from air during the entire RPA on-site testing process, thereby avoiding aerosol contamination of the reaction product caused by opening the cartridge during amplification and sample addition. Furthermore, the device is easy to carry and operate, and allows for easy observation of experimental results. This significantly improves testing efficiency during RPA on-site testing, further meeting the needs of rapid on-site RPA testing.

[0051] In one embodiment, Figure 2 As shown, a test strip 204 for receiving the mixed liquid is provided in the first housing 201. Preferably, the test strip 204 is arranged in the first housing 201 along a longitudinal extension to help improve the accuracy of the detection.

[0052] In the present invention, the guide groove 203 can lift the floating platform 20 upward until the floating platform 20 enters the calibration chamber 13, thereby connecting the calibration chamber 13 with the first cartridge 201. From then on, the amplification product and the calibration liquid form a mixed liquid in the calibration chamber 13, which can enter the first cartridge 201 through the guide cavity 15 and the guide groove 203 and crawl on the test strip 204 to complete the RPA on-site detection work.

[0053] Preferably, when observing the test paper results for judgment, if two red lines appear, it is positive, and if one red line appears, it is negative.

[0054] Compared with the prior art, the advantages of the present invention are:

[0055] The present invention uses an auxiliary device 100 in conjunction with a visual test card 200, so that the amplification product can be mixed with the calibration liquid through mechanical movement, and the floating platform 20 is lifted up by the guide groove 203 to allow the mixed liquid formed by the amplification product and the calibration liquid to crawl on the test strip 204, and then the result can be interpreted by the change of the test strip 204. In addition, during the entire RPA on-site detection process, it can be ensured that the amplification product will not come into contact with the outside air, thereby avoiding the problem of reaction product aerosol contamination caused by opening the cover during the amplification and sample addition process. In addition, the present device has the function of being easy to carry and operate and easy to observe the experimental results. It can effectively improve the detection efficiency during RPA on-site detection, and further meet the needs of RPA on-site rapid detection.

[0056] In the description of this utility model, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, features specified as "first" or "second" may explicitly or implicitly include one or more of such features.

[0057] In the present invention, unless otherwise clearly specified and limited, terms such as "installed", "connected", "connected", and "fixed" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can be the internal connection of two components.

[0058] For those skilled in the art, the specific meanings of the above terms in this utility model can be understood according to specific circumstances.

[0059] The above are merely preferred embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Those skilled in the art may easily make changes or modifications within the scope disclosed herein, and such changes or modifications shall be encompassed within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be subject to the scope of protection of the claims.

Claims

1. An auxiliary device for visual detection card, characterized in that, include: A box body (10) for engaging with a visual detection card (200), wherein a receiving chamber is provided in the box body (10), a PCR tube (11) adapted to the containing chamber, and a cutting member (12) disposed in the accommodating chamber and used for cutting the PCR tube (11), The auxiliary device further comprises a calibration chamber (13) disposed below the cutting member (12) and containing a calibration liquid, and a float (14) for supporting the cutting member (12) and separating the containing chamber from the calibration chamber (13).

2. The auxiliary device for visual detection card according to claim 1, characterized in that: The accommodating chambers are provided with two and are arranged at intervals, and the PCR tube (11) consists of a first test tube containing an amplification product and a second test tube configured as an empty tube.

3. The auxiliary device for visual detection card according to claim 2, characterized in that: The float plug (14) is configured as a cylindrical structure, and the outer peripheral surface of the float plug (14) forms a sealed connection with the inner peripheral surface of the accommodating chamber.

4. The auxiliary device for visual detection card according to claim 3, characterized in that: The cutting piece (12) is constructed in the form of a prism and is fixed to the upper end surface of the floating plug (14). The PCR tube (11) is configured to move downward under the action of an external force and be cut by the cutting member (12) until the float (14) is pushed downward to allow the accommodating chamber to communicate with the calibration chamber (13), thereby forming a mixed liquid with the amplification product and the calibration liquid in the calibration chamber (13).

5. The auxiliary device for visual detection card according to claim 4, characterized in that: The outer diameter of the PCR tube (11) is no greater than the inner diameter of the accommodating chamber.

6. The auxiliary device for visual detection card according to claim 5, characterized in that: A guide cavity (15) is provided in the box body (10) and below the calibration chamber (13). The auxiliary device further comprises a floating platform (20) arranged in the guide cavity (15) and used for sealing the calibration chamber (13).

7. The auxiliary device for visual detection card according to claim 6, characterized in that: The floating platform (20) includes a sealing portion (21) forming a sealed connection with the guide cavity (15), and a limiting portion (22) arranged above the sealing portion (21) and used to abut against the PCR tube (11).

8. A detection device, characterized in that: It comprises a visual detection card (200) and an auxiliary device according to any one of claims 1 to 7, which is matched with the visual detection card (200).

9. The detection device according to claim 8, characterized in that The visual detection card (200) comprises a first card shell (201), a second card shell (202) adapted to the first card shell (201), and a guide groove (203) longitudinally arranged on the second card shell (202). The guide groove (203) is configured to lift the floating platform (20) so that the mixed liquid flows into the first cartridge (201) through the guide groove (203).

10. The detection device according to claim 9, characterized in that: A test strip (204) extending longitudinally and used for receiving the mixed liquid is provided in the first housing (201).

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