Multi-channel PCR (Polymerase Chain Reaction) detection device

Abstract

The utility model belongs to the field of molecular detection, and particularly relates to a multichannel PCR detection device which is characterized in that a heating module for heating a reaction tube is arranged on a radiator of a heat dissipation module, an optical detection module is arranged on one side of an air inlet of a heat dissipation fan, and the heat dissipation module is utilized, so that the heat dissipation efficiency is improved through reasonable layout; according to the multi-channel PCR detection device, the heating module and the optical detection module are cooled at the same time, the structure is simple, the heat dissipation cost is saved, and due to the fact that only one heat dissipation module is adopted, the whole multi-channel PCR detection device is more compact in structure, and miniaturization design of products is facilitated.

Description

Technical Field

[0001] The present application relates to the field of molecular detection technology, and in particular to a multi-channel PCR detection device. Background Art

[0002] Molecular diagnostic technology uses DNA and RNA as diagnostic materials and molecular biology techniques to diagnose human conditions and diseases by detecting the presence, defects, or abnormal expression of genes. PCR (polymerase chain reaction) technology is a molecular biology technique used to amplify specific DNA fragments (genes to be tested) in a sample, i.e., specific in vitro amplification of DNA fragments. The basic principle of PCR is similar to the natural DNA replication process, consisting of three basic reaction steps: denaturation, annealing, and extension. DNA is denatured by increasing the temperature, and the polymerase enzyme replicates the single strand into a double strand, thereby achieving gene replication.

[0003] Quantitative Real-time Polymerase Chain Reaction (qPCR) is a method of adding a reporter group to a specific DNA fragment in the PCR reaction system of the sample to be tested. The intensity of the fluorescent signal emitted by the reporter group increases each time the specific DNA fragment undergoes a reaction cycle (that is, after undergoing one replication). By detecting the change in the fluorescence signal intensity after each reaction cycle, the change in the amount of the reaction product can be monitored in real time. Based on the monitoring results, qualitative and quantitative analysis of the sample to be tested can be performed.

[0004] The PCR instrument is a key instrument for implementing PCR technology. Existing PCR instruments usually include a housing and a temperature control module and an optical detection module installed in the housing. During the operation of the PCR instrument, the heating module and the optical detection module generate a lot of heat, which requires heat dissipation to ensure stable operation of the PCR instrument. In existing PCR instruments, heat dissipation holes are usually set on the housing, and cooling fans are respectively configured for the heating module and the optical detection module for heat dissipation. The large number of cooling fans is not conducive to cost savings and miniaturization of product design. Utility Model Content

[0005] The embodiment of the present application provides a multi-channel PCR detection device, which is used to solve the problem that the existing PCR instrument has a large number of cooling fans, which is not conducive to cost saving and miniaturization of the product.

[0006] To achieve the above objectives, the present application provides a multi-channel PCR detection device, comprising:

[0007] A housing having heat dissipation holes;

[0008] a heat dissipation module disposed in the housing, the heat dissipation module comprising a heat dissipation fan and a radiator disposed on one side of an air outlet of the heat dissipation fan;

[0009] A heating module, comprising a heating plate disposed on the radiator and a heat-conducting base disposed on the heating plate, wherein the heat-conducting base has a heat-conducting groove for accommodating a reaction tube, and a avoidance hole corresponding to the heat-conducting groove is provided on the top of the housing; and

[0010] An optical detection module is arranged inside the housing and located on one side of the air inlet of the cooling fan. The optical detection module is connected to the interior of the heat conduction groove through an optical fiber and is used to detect the optical signal of the liquid in the reaction tube.

[0011] Optionally, the heat dissipation holes include air inlet holes and air outlet holes respectively arranged on opposite sides of the shell, and the optical detection module, the heat dissipation fan and the radiator are arranged in sequence inside the shell from the air inlet holes to the air outlet holes.

[0012] Optionally, the radiator has a plurality of heat dissipation fins, and the heat dissipation fan can drive airflow to pass through the heat dissipation fins and make heat exchange contact with the surfaces of the heat dissipation fins.

[0013] Optionally, the thermally conductive base has a plurality of thermally conductive grooves arranged in an array.

[0014] Optionally, the sixteen heat-conducting grooves are arranged in four rows and four columns.

[0015] Optionally, the heating plate includes a Peltier, and the heat conductive base is arranged on the hot surface of the Peltier.

[0016] Optionally, the heating module further includes a first graphite sheet sandwiched between the lower surface of the heating plate and the heat sink, and a second graphite sheet sandwiched between the upper surface of the heating plate and the thermally conductive base.

[0017] Optionally, the shell includes a base and an upper cover arranged on the base, the heat dissipation module and the optical detection module are installed on the base, the heat dissipation holes are arranged on the side of the upper cover, and the avoidance holes are arranged on the top of the upper cover.

[0018] Optionally, the thermally conductive base, the heating plate and the radiator are stacked in sequence from top to bottom, the radiator is provided with a threaded hole, the thermally conductive base and the heating plate are provided with avoidance holes corresponding to the threaded holes, the screw of the fastening screw passes through the avoidance holes from top to bottom and is screwed to the threaded hole, and an elastic member is provided between the screw head of the fastening screw and the upper surface of the thermally conductive base.

[0019] Optionally, the elastic member includes a spring, which is sleeved on the portion of the screw of the fastening screw located outside the avoidance through hole, and the two ends of the spring elastically press against the screw head of the fastening screw and the upper surface of the thermal conductive base respectively.

[0020] The beneficial effects of the multi-channel PCR detection device provided by the present application are: compared with the existing technology, the multi-channel PCR detection device of the present application arranges the heating module for heating the reaction tube on the radiator of the heat dissipation module, and arranges the optical detection module on the air inlet side of the heat dissipation fan. By utilizing one heat dissipation module and reasonable layout, heat dissipation and cooling of the heating module and the optical detection module are simultaneously achieved, the structure is simple, and heat dissipation costs are saved. Since only one heat dissipation module is used, the structure of the entire multi-channel PCR detection device is more compact, which is conducive to the miniaturized design of the product. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0022] in:

[0023] Figure 1 Schematic diagram of the external structure of a multi-channel PCR detection device according to one embodiment of the present application;

[0024] Figure 2 1 is a schematic cross-sectional view of a multi-channel PCR detection device according to an embodiment of the present application;

[0025] Figure 3 yes Figure 2 A schematic diagram of the structure enlargement at point A;

[0026] Figure 4 This is a schematic diagram of the external structure of a multi-channel PCR detection device shown in one embodiment of the present application with the upper cover removed;

[0027] Figure 5 This is a schematic diagram of the heat sink structure of the heat dissipation module in the multi-channel PCR detection device shown in one embodiment of the present application.

[0028] Description of main component symbols:

[0029] 100, housing; 101, air inlet; 102, air outlet; 103, avoidance hole; 110, base; 120, upper cover;

[0030] 200, heat dissipation module; 210, cooling fan; 220, radiator; 221, threaded hole;

[0031] 300, heating module; 310, heating plate; 320, heat-conducting base; 321, heat-conducting groove;

[0032] 400, optical detection module;

[0033] 500, fastening screws;

[0034] 600. Elastic parts. DETAILED DESCRIPTION

[0035] To facilitate understanding of the present application, the present application will be described more fully below with reference to the accompanying drawings. The accompanying drawings illustrate preferred embodiments of the present application. However, the present application may be implemented in many other forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a more thorough and comprehensive understanding of the disclosure of the present application.

[0036] It should be noted that when an element is referred to as being “fixed on” or “disposed on” another element, it may be directly on the other element or indirectly on the other element. When an element is referred to as being “connected to” another element, it may be directly connected to the other element or indirectly connected to the other element.

[0037] It should be understood that the terms "length," "width," "up," "down," "front," "back," "left," "right," "vertical," "horizontal," "top," "bottom," "inside," "outside," and the like, indicating positions or location relationships, are based on the positions or location relationships shown in the accompanying drawings and are intended only to facilitate the description of this application and simplify the description. They do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limiting this application. In the description of this application, "plurality" means two or more, unless otherwise expressly and specifically defined.

[0038] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application pertains. The terms used herein in the specification of this application are for the purpose of describing specific embodiments only and are not intended to limit this application.

[0039] It should also be noted that, in the embodiments of the present application, the same figure mark represents the same component or the same part. For the same parts in the embodiments of the present application, the figure may only mark one of the parts or components as an example. It should be understood that the figure mark also applies to other identical parts or components.

[0040] The embodiment of the present application provides a multi-channel PCR detection device, such as Figure 1-Figure 2 As shown, the multi-channel PCR detection device includes a housing 100, a heat dissipation module 200, a heating module 300 and an optical detection module 400. The housing 100 is provided with heat dissipation holes, and the heat dissipation module 200 is arranged in the housing 100. The heat dissipation module 200 includes a heat dissipation fan 210 and a radiator 220 arranged on one side of the air outlet of the heat dissipation fan 210. The heating module 300 includes a heating plate 310 arranged on the radiator 220 and a heat-conducting base 320 arranged on the heating plate 310. The heat-conducting base 320 has a heat-conducting groove 321 for accommodating a reaction tube, and the top of the housing 100 is provided with an avoidance hole 103 corresponding to the heat-conducting groove 321. The optical detection module 400 is arranged inside the housing 100 and is located on one side of the air inlet of the heat dissipation fan 210. The optical detection module 400 is connected to the inside of the heat-conducting groove 321 through an optical fiber and is used to detect the optical signal of the liquid in the reaction tube.

[0041] In an embodiment of the present application, the multi-channel PCR detection device arranges a heating module 300 for heating the reaction tube on the radiator 220 of the heat dissipation module 200, and arranges the optical detection module 400 on the air inlet side of the heat dissipation fan 210. By utilizing one heat dissipation module 200 and through the above-mentioned reasonable layout, heat dissipation and cooling of the heating module 300 and the optical detection module 400 are simultaneously achieved. The structure is simple and the heat dissipation cost is saved. Since only one heat dissipation module 200 is used, the structure of the entire multi-channel PCR detection device is more compact, which is conducive to the miniaturized design of the product.

[0042] In one embodiment, if Figure 1-Figure 2 As shown, the heat dissipation holes include an air inlet 101 and an air outlet 102 respectively arranged on opposite sides of the shell 100, and the optical detection module 400, the heat dissipation fan 210 and the radiator 220 are arranged in sequence inside the shell 100 from the air inlet 101 to the air outlet 102.

[0043] Through the above arrangement, during the operation of the multi-channel PCR detection device, the optical detection module 400 and the heating module 300 generate heat, the cooling fan 210 is started, and negative pressure is generated at its air inlet, so that the air outside the outer shell 100 is drawn into the outer shell 100 through the air inlet 101 on one side of the outer shell 100. Under the action of the cooling fan 210, the air flows through the optical detection module 400 and the radiator 220 in turn and is discharged from the air outlet 102 on the other side of the outer shell 100, taking away the heat on the optical detection module 400 and the radiator 220, thereby fanning the heat of the optical detection module 400 and the heating module 300.

[0044] For ease of understanding, when the cooling fan 210 is working, the gas flows in Figure 2 Indicated by arrows.

[0045] In one embodiment, combining Figure 2 and Figure 4 As shown, the radiator 220 has a plurality of radiating fins, and the radiating fan 210 can drive airflow to pass through the radiating fins and make heat exchange contact with the surface of the radiating fins, thereby improving the heat dissipation capacity.

[0046] As can be understood, heat sink 220 is a device used to dissipate heat from heat-prone electronic components within an appliance. It is typically made of aluminum alloy, brass, or bronze. Heat sink 220 includes a mounting plate and multiple cooling fins integrally formed on the lower surface of the mounting plate. The cooling fins are parallel to each other and perpendicular to the mounting plate. The air flow provided by the fan is parallel to the cooling fins to improve the heat transfer efficiency of the flowing air.

[0047] In one embodiment, if Figure 4 As shown, the thermal base 320 has a plurality of thermally conductive grooves 321 arranged in an array. Preferably, sixteen thermally conductive grooves 321 are arranged in four rows and four columns, thereby forming a 16-channel PCR detection device. This configuration can accommodate single reaction tubes, 4-tube strips, and even 8-tube strips cut apart, providing high adaptability and broad customer appeal.

[0048] In one embodiment, the heating plate 310 includes a Peltier, and the thermal conductive base 320 is arranged on the hot surface of the Peltier. The thermal conductive base 320 can be made of a material with good thermal conductivity such as aluminum or copper. The hot surface of the Peltier provides heat for the reaction tube placed in the heat conductive groove 321.

[0049] It is conceivable that in other embodiments, the heating plate 310 may also be an electric heating plate or an electric heating film.

[0050] Preferably, the heating module 300 further includes a first graphite sheet sandwiched between the lower surface of the heating plate 310 and the heat sink 220, and a second graphite sheet sandwiched between the upper surface of the heating plate 310 and the thermally conductive base 320. The first and second graphite sheets improve the heat transfer efficiency between the heating plate 310, the heat sink 220, and the thermally conductive base 320. Of course, thermal grease may also be applied between the heating plate 310 and the heat sink 220, and between the heating plate 310 and the thermally conductive base 320.

[0051] In one embodiment, if Figure 1-Figure 2 As shown, the housing 100 includes a base 110 and an upper cover 120 arranged on the base 110, the heat dissipation module 200 and the optical detection module 400 are installed on the base 110, the heat dissipation holes are arranged on the side of the upper cover 120, and the avoidance hole 103 is arranged on the top of the upper cover 120.

[0052] By making the housing 100 comprised of a base 110 and an upper cover 120 , the installation of the heat dissipation module 200 , the heating module 300 and the optical detection module 400 in the housing 100 and their subsequent maintenance and replacement are facilitated during product production.

[0053] The upper cover 120 and the base 110 can be connected by buckles or screws. A display screen or a touch screen can also be provided on the upper cover 120.

[0054] In some embodiments, as Figure 2-Figure 3 As shown, the thermal base 320, heating plate 310, and heat sink 220 are stacked in sequence from top to bottom. Threaded holes 221 are provided on the heat sink 220. Avoidance holes corresponding to the threaded holes 221 are provided on the thermal base 320 and heating plate 310. The diameter of the avoidance holes is larger than that of the threaded holes 221 on the heat sink 220. The screw of the fastening screw 500 passes through the avoidance holes from top to bottom and is screwed into the threaded holes 221. An elastic member 600 is provided between the screw head of the fastening screw 500 and the upper surface of the thermal base 320.

[0055] The elastic member 600 generates elastic downward pressure on the thermal base 320. The elastic pressurization method can effectively adapt to the thermal expansion and contraction between the heating plate 310, the thermal base 320 and the radiator 220, ensuring a good and stable heat conduction effect without causing damage to the heating plate 310.

[0056] In a specific embodiment, Figure 2-Figure 3 As shown, the elastic member 600 includes a spring, which is sleeved on the portion of the screw of the fastening screw 500 outside the avoidance through hole, and the two ends of the spring elastically press against the screw head of the fastening screw 500 and the upper surface of the thermal conductive base 320 respectively.

[0057] The use of a spring as the elastic member 600 for applying downward pressure to the thermal base 320 has a simple structure and is easy to set up. It can be imagined that in other implementations, the elastic member 600 can also use a rubber pad / rubber ring with good elasticity. Specifically, the rubber pad / rubber ring can be made of silicone, rubber or latex.

[0058] The technical features of the above embodiments can be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0059] The above embodiments merely illustrate several implementation methods of the present application. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present application. It should be noted that a person of ordinary skill in the art may make various modifications and improvements without departing from the spirit of the present application, all of which fall within the scope of protection of the present application. Therefore, the scope of protection of the present application shall be determined by the appended claims.

Claims

1. A multi-channel PCR detection device, characterized in that: include: A housing (100), wherein the housing (100) is provided with heat dissipation holes; A heat dissipation module (200) is disposed in the housing (100), and the heat dissipation module (200) comprises a heat dissipation fan (210) and a radiator (220) disposed on one side of an air outlet of the heat dissipation fan (210); A heating module (300) comprises a heating plate (310) arranged on the radiator (220) and a heat-conducting base (320) arranged on the heating plate (310), wherein the heat-conducting base (320) has a heat-conducting groove (321) for accommodating a reaction tube, and a avoidance hole (103) corresponding to the heat-conducting groove (321) is provided on the top of the housing (100); and An optical detection module (400) is arranged inside the housing (100) and located on one side of the air inlet of the cooling fan (210). The optical detection module (400) is connected to the interior of the heat conduction groove (321) through an optical fiber and is used to detect the optical signal of the liquid in the reaction tube.

2. The multi-channel PCR detection device according to claim 1, characterized in that The heat dissipation holes include an air inlet hole (101) and an air outlet hole (102) respectively arranged on opposite sides of the housing (100); the optical detection module (400), the heat dissipation fan (210) and the radiator (220) are arranged in sequence from the air inlet hole (101) to the air outlet hole (102) inside the housing (100).

3. The multi-channel PCR detection device according to claim 1, characterized in that: The radiator (220) has a plurality of radiating fins, and the radiating fan (210) can drive airflow to pass through the radiating fins and to make heat exchange contact with the surfaces of the radiating fins.

4. The multi-channel PCR detection device according to claim 1, characterized in that The heat-conducting base (320) has a plurality of heat-conducting grooves (321) arranged in an array.

5. The multi-channel PCR detection device according to claim 4, characterized in that: The sixteen heat-conducting grooves (321) are arranged in four rows and four columns.

6. The multi-channel PCR detection device according to claim 1, characterized in that: The heating plate (310) includes a Peltier, and the heat-conducting base (320) is arranged on the hot surface of the Peltier.

7. The multi-channel PCR detection device according to claim 1, characterized in that: The heating module (300) further includes a first graphite sheet sandwiched between the lower surface of the heating plate (310) and the heat sink (220), and a second graphite sheet sandwiched between the upper surface of the heating plate (310) and the heat conducting base (320).

8. The multi-channel PCR detection device according to claim 1, characterized in that: The housing (100) comprises a base (110) and an upper cover (120) arranged on the base (110); the heat dissipation module (200) and the optical detection module (400) are installed on the base (110); the heat dissipation holes are arranged on the side of the upper cover (120); and the avoidance hole (103) is arranged on the top of the upper cover (120).

9. The multi-channel PCR detection device according to any one of claims 1 to 8, characterized in that: The thermal conductive base (320), the heating plate (310) and the radiator (220) are stacked in sequence from top to bottom, the radiator (220) is provided with a threaded hole (221), the thermal conductive base (320) and the heating plate (310) are provided with avoidance holes corresponding to the threaded holes (221), the screw of the fastening screw (500) passes through the avoidance holes from top to bottom and is screwed to the threaded hole (221), and an elastic member (600) is provided between the screw head of the fastening screw (500) and the upper surface of the thermal conductive base (320).

10. The multi-channel PCR detection device according to claim 9, characterized in that: The elastic member (600) includes a spring, which is sleeved on the portion of the screw of the fastening screw (500) located outside the avoidance through hole, and the two ends of the spring elastically press against the screw head of the fastening screw (500) and the upper surface of the heat-conducting base (320) respectively.

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