Temperature measuring device in reaction cavity suitable for micro-fluidic chip

Abstract

The utility model provides a temperature measuring device in a reaction cavity suitable for a micro-fluidic chip, and the device comprises a temperature measuring device main body which is used for bearing all devices; the plurality of reaction chambers are arranged at the lower part of the temperature measuring device main body; the upper part of each reaction chamber is provided with a wire outlet groove, and the wire outlet grooves are connected with the reaction chambers and the outer part of the temperature measuring device main body; the temperature sensor is arranged in the reaction chamber and is smaller than the reaction chamber in volume; and one end of the temperature sensor wire is connected with the temperature sensor and the other end is connected with a corresponding instrument. The temperature measuring device in the reaction cavity suitable for the micro-fluidic chip solves the problems that the existing equipment cannot accurately control the temperature, is low in amplification efficiency and is easy to cause wrong detection results, and has the advantages of accurate temperature control, high amplification efficiency, accurate detection results and difficulty in making mistakes.

Description

Technical Field

[0001] The utility model relates to the technical field of temperature detection, in particular to a temperature measuring device suitable for a reaction cavity of a microfluidic chip. Background Art

[0002] In vitro diagnostics, or IVD (In Vitro Diagnosis), refers to products and services that obtain clinical diagnostic information by testing human samples (blood, body fluids, tissues, etc.) outside the human body, and then judge diseases or body functions.

[0003] Microfluidics chip technology (Microfluidics) integrates the basic operating units of sample preparation, reaction, separation, detection, etc. in the biological, chemical, and medical analysis processes onto a micron-scale chip to automatically complete the entire analysis process. When using microfluidic chips, because they require a small amount of sample and reagents, the liquid temperature rises and falls very quickly, and the amplification speed during detection is very fast, so the detection efficiency is higher than other nucleic acid detection methods. However, less liquid will cause a large difference between the temperature at the thermal cycle position and the temperature of the reagent in the amplification chamber. The conventional temperature detection method is to place the temperature probe at the heating position. Due to the difference between the heating temperature and the temperature of the liquid in the amplification chamber, it is impossible to accurately judge the actual temperature of the reagent, and it is difficult to achieve precise temperature control, resulting in low amplification efficiency and prone to erroneous test results. Utility Model Content

[0004] The main purpose of the utility model is to provide a temperature measuring device for a reaction chamber of a microfluidic chip, which overcomes the above technical problems.

[0005] In order to achieve the above purpose, the present invention proposes the following technical solutions:

[0006] A temperature measuring device for a reaction chamber of a microfluidic chip, comprising:

[0007] The main body of the temperature measuring device is used to carry various devices;

[0008] Reaction chambers, multiple reaction chambers are arranged at the lower part of the temperature measuring device body;

[0009] Outlet slot: at least one outlet slot is provided above each reaction chamber, connecting the reaction chamber with the outside of the temperature measuring device body;

[0010] The temperature sensor is arranged inside the reaction chamber and has a volume smaller than the reaction chamber;

[0011] Temperature sensor wire, one end is connected to the temperature sensor, and the other end is connected to the corresponding instrument.

[0012] Furthermore, an X-axis positioning device is provided on the main body of the temperature measuring device.

[0013] Furthermore, a Z-axis positioning device is provided on the main body of the temperature measuring device.

[0014] Furthermore, the gap between the reaction chamber and the temperature sensor is filled with a high thermal conductivity material.

[0015] Furthermore, the reaction chamber protrudes from the temperature measuring device body to form a bulge.

[0016] Furthermore, the temperature sensor adopts a high-precision sensor.

[0017] Furthermore, high-precision sensors include but are not limited to PT100, PT1000, thermocouples, and thermistors.

[0018] Furthermore, the X-axis positioning device adopts an arc design and is integrated with the main body of the temperature measuring device.

[0019] Furthermore, the Z-axis positioning device is integrated with the temperature measuring device body.

[0020] Furthermore, the side surfaces of the temperature measuring device body are covered with a waterproof film.

[0021] The utility model provides a temperature measuring device for the reaction chamber of a microfluidic chip, which solves the problems of the existing equipment in being unable to accurately control the temperature, having low amplification efficiency, and being prone to erroneous detection results. The device has the advantages of accurate temperature control, high amplification efficiency, accurate detection results, and being less prone to errors. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The drawings constituting part of this application are provided to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are provided to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0023] Figure 1 This is an overall structural diagram of a temperature measuring device in a reaction chamber suitable for a microfluidic chip according to the present invention.

[0024] Figure 2 This is a front view of a temperature measuring device in a reaction chamber of a microfluidic chip according to the present invention.

[0025] Figure 3 This is a side view of a temperature measuring device in a reaction chamber of a microfluidic chip according to the present invention.

[0026] The above drawings include the following reference numerals:

[0027] 01. Temperature sensor wire; 02. Temperature measuring device body; 03. Z-axis positioning; 04. X-axis positioning; 05. Temperature sensor; 06. Protrusion; 07. Wire outlet slot; 08. Reaction chamber. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is in no way intended to limit the present invention and its application or use. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0029] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or combinations thereof.

[0030] Unless otherwise specifically stated, the relative arrangement of the parts and steps, the numerical expressions and the numerical values ​​set forth in these embodiments do not limit the scope of the present invention. At the same time, it should be understood that, for ease of description, the sizes of the various parts shown in the drawings are not drawn according to the actual proportional relationship. The techniques, methods and equipment known to those of ordinary skill in the relevant art may not be discussed in detail, but where appropriate, the techniques, methods and equipment should be considered as part of the authorization specification. In all examples shown and discussed here, any specific values ​​should be interpreted as being merely exemplary and not as limitations. Therefore, other examples of the exemplary embodiments may have different values. It should be noted that similar numbers and letters represent similar items in the following figures, and therefore, once an item is defined in one figure, it does not need to be further discussed in subsequent figures.

[0031] Reference below Figures 1 to 3 , the utility model is further described:

[0032] A temperature measuring device for a reaction chamber of a microfluidic chip, comprising:

[0033] The temperature measuring device body 02 is used to carry various devices;

[0034] Reaction chambers 08, multiple reaction chambers 08 are arranged at the lower part of the temperature measuring device body 02;

[0035] Outlet slot 07: at least one outlet slot 07 is provided above each reaction chamber 08, connecting the reaction chamber 08 with the outside of the temperature measuring device body 02;

[0036] The temperature sensor 05 is disposed inside the reaction chamber 08 and has a volume smaller than the reaction chamber 08;

[0037] One end of the temperature sensor wire 01 is connected to the temperature sensor 05, and the other end is connected to the corresponding instrument.

[0038] The temperature measuring device body 02 is made of non-metallic materials. The structure of the temperature measuring device body 02 is as follows: Figure 1 As shown, a reaction chamber 08 is provided on the temperature measuring device body 02.

[0039] In this embodiment, each reaction chamber 08 includes two wire outlet slots 07, which are connected to the reaction chamber 08 to facilitate the passage and avoidance of wires, ensuring that both sides of the temperature measuring device body 02 are flat, which is convenient for bonding with heating components.

[0040] The temperature measuring device body 02 is provided with an X-axis positioning device 04 and a Z-axis positioning device 03, both of which are integrated with the temperature measuring device body 02.

[0041] The Z-axis positioning device 03 is integral with the temperature measuring device body 02, facilitating precise positioning of the entire chip temperature measuring device. It assists with precise Z-axis positioning during use, ensuring the detection device maintains the same vertical position when testing different instruments. The X-axis positioning device 04 is integral with the temperature measuring device body 02 and utilizes a circular arc design, facilitating precise positioning and overall positioning accuracy. It assists with X-axis positioning during use, ensuring the detection device maintains the same horizontal position when testing different instruments. This design offers the advantages of ensuring precise positioning during use, reducing insertion friction, and increasing repeatability.

[0042] The gap between the reaction chamber 08 and the temperature sensor 05 is filled with a high thermal conductivity material 06 . The reaction chamber 08 protrudes from the temperature measuring device body 02 to form a protrusion 06 .

[0043] Temperature sensor 05 is smaller than reaction chamber 08. The remaining space is filled and encapsulated with a highly thermally conductive material, ensuring a seamless, gap-free structure. This material forms a protrusion 06 protruding outward from the reaction chamber. This protrusion is then bonded or welded to the side of temperature measurement device body 02 with a heat-resistant, waterproof film. This film effectively reduces thermal resistance and facilitates heat conduction. This protrusion 06 ensures more effective contact between the temperature measurement point and the heating element, ensuring good heat conduction and obtaining accurate temperature measurements within reaction chamber 08 to support subsequent data analysis.

[0044] The temperature measuring device body 02 includes multiple reaction chambers 08, each of which contains a temperature sensor 05. The sensor is a high-precision temperature sensor (can be PT100, PT1000, thermocouple, thermistor, etc.), which is used to accurately detect the internal temperature of each reaction chamber 08. The corresponding electrical signal is derived through the temperature sensor wire 01 to obtain high-precision temperature information of each hole position. This temperature information can be used to monitor and calculate parameters such as the temperature rise and fall speed, temperature accuracy, temperature repeatability, and temperature uniformity in the chip. It can also be used for consistency debugging between instruments in production and measurement calibration before delivery.

[0045] In the description of the present invention, it needs to be understood that the directions or positional relationships indicated by directional words such as "front, back, up, down, left, right", "horizontal, vertical, vertical, horizontal" and "top, bottom" are usually based on the directions or positional relationships shown in the drawings. They are only for the convenience of describing the present invention and simplifying the description. Unless otherwise specified, these directional words do not indicate or imply that the device or element referred to must have a specific direction or be constructed and operated in a specific direction. Therefore, they cannot be understood as limiting the scope of protection of the present invention; the directional words "inside and outside" refer to the inside and outside relative to the outline of each component itself.

[0046] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that the present invention is susceptible to various modifications and variations. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A temperature measuring device for a reaction chamber of a microfluidic chip, characterized in that: include: The temperature measuring device body (02) is used to carry various devices; Reaction chambers (08), wherein a plurality of the reaction chambers (08) are arranged at the lower portion of the temperature measuring device body (02); A wire outlet slot (07), at least one wire outlet slot (07) is provided above each reaction chamber (08), connecting the reaction chamber (08) with the outside of the temperature measuring device body (02); A temperature sensor (05) is disposed inside the reaction chamber (08) and has a volume smaller than that of the reaction chamber (08); The temperature sensor wire (01) has one end connected to the temperature sensor (05) and the other end connected to a corresponding instrument.

2. The temperature measuring device for a reaction chamber of a microfluidic chip according to claim 1, characterized in that: An X-axis positioning device (04) is provided on the temperature measuring device body (02).

3. The temperature measuring device for a reaction chamber of a microfluidic chip according to claim 2, characterized in that: A Z-axis positioning device (03) is provided on the temperature measuring device body (02).

4. The temperature measuring device for a reaction chamber of a microfluidic chip according to claim 1, characterized in that: The gap between the reaction chamber (08) and the temperature sensor (05) is filled with a high thermal conductivity material.

5. The temperature measuring device for a reaction chamber of a microfluidic chip according to claim 1, characterized in that: The reaction chamber (08) protrudes from the temperature measuring device body (02) to form a protrusion (06).

6. The temperature measuring device for a reaction chamber of a microfluidic chip according to claim 1, characterized in that: The temperature sensor (05) is a high-precision sensor.

7. The temperature measuring device for a reaction chamber of a microfluidic chip according to claim 6, characterized in that: The high-precision sensors include but are not limited to PT100, PT1000, thermocouples, and thermistors.

8. The temperature measuring device for a reaction chamber of a microfluidic chip according to claim 2, characterized in that: The X-axis positioning device (04) adopts an arc design and is integrally arranged with the temperature measuring device body (02).

9. The temperature measuring device for a reaction chamber of a microfluidic chip according to claim 3, characterized in that: The Z-axis positioning device (03) is integrally arranged with the temperature measuring device body (02).

10. The temperature measuring device in a reaction chamber of a microfluidic chip according to claim 1, characterized in that: The side surface of the temperature measuring device body (02) is covered with a waterproof film.

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