Centrifugal equipment for separating umbilical cord mesenchymal stem cells

Abstract

The centrifugal equipment comprises a main body, a centrifugal assembly and a connecting mechanism, the main body comprises a machine body, a centrifugal cavity is formed in the top of the machine body, a partition plate is fixedly connected to the inner side of the centrifugal cavity, a driving motor is fixedly connected to the bottom of the partition plate, and a rotating shaft of the driving motor penetrates through the partition plate and is rotationally connected with the partition plate; the top end of a rotating shaft of the driving motor is fixedly connected with a tray, the top of the tray is fixedly connected with three sets of positioning columns, the center of the top of the tray is fixedly connected with a threaded column, and the front side of the machine body is fixedly connected with a control panel used for controlling the device to operate. The centrifugal machine has the beneficial effects that the connecting mechanism is arranged, three groups of clamping columns are matched with the positioning plate to lock the locking nut, so that the locking nut cannot rotate, the situation that the locking nut is excessively tightened or loosened after long-term use is avoided, and the centrifugal stability is guaranteed.

Description

Technical Field

[0001] The utility model relates to the technical field of cell separation, in particular to a centrifugal device for separating umbilical cord mesenchymal stem cells. Background Art

[0002] Umbilical cord mesenchymal stem cells (UCMSCs) are multipotent stem cells found in the umbilical cord tissue of newborns. They possess the ability to self-renew and differentiate into various tissue cell types, such as bone cells, cartilage cells, and adipocytes. Therefore, they hold broad clinical application prospects. Umbilical cord MSC isolation involves extracting and purifying these cells from the umbilical cord tissue of newborns through a series of procedures, which require the use of centrifugal equipment.

[0003] At present, the centrifugal device commonly used for umbilical cord mesenchymal stem cell separation has a centrifugal turntable that is mainly fixed to the shaft by bolts. Depending on the rotation direction of the turntable, this fastening method is prone to problems such as overtightening or loosening of the bolts after long-term use. Overtightening will make it difficult to subsequently remove and replace the turntable, and may also damage the bolts or shaft, affecting the normal operation of the equipment. Looseness may cause instability during the centrifugation process, thereby affecting the effect and safety of stem cell separation. Therefore, a centrifugal device for umbilical cord mesenchymal stem cell separation is proposed. Utility Model Content

[0004] The purpose of this section is to summarize some aspects of the embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and in the abstract and title of the utility model to avoid obscuring the purpose of this section, the abstract and the title of the utility model, and such simplifications or omissions shall not be used to limit the scope of the present invention.

[0005] In view of the above problems of the centrifugal device for separating umbilical cord mesenchymal stem cells, the present utility model is proposed.

[0006] To solve the above technical problems, the present invention provides the following technical solutions: a centrifugal device for separating umbilical cord mesenchymal stem cells, comprising:

[0007] The main body includes a body, a centrifugal chamber is provided on the top of the body, a partition is fixedly connected to the inside of the centrifugal chamber, a drive motor is fixedly connected to the bottom of the partition, a shaft of the drive motor passes through the partition and is rotatably connected to the partition, a tray is fixedly connected to the top of the drive motor shaft, three groups of positioning columns are fixedly connected to the top of the tray, a threaded column is fixedly connected to the center of the top of the tray, and a control panel for controlling the operation of the device is fixedly connected to the front side of the body;

[0008] The centrifugal assembly includes a centrifugal table, a plurality of centrifuge tube slots are provided on the top of the centrifugal table, the centrifugal table is arranged above the tray, the centrifugal table is provided with positioning grooves at positions opposite to the three groups of positioning posts, the centrifugal table is provided with through holes at positions opposite to the threaded posts, the three groups of positioning posts are respectively inserted into the three groups of positioning grooves, and the threaded posts pass through the through holes;

[0009] The connecting mechanism comprises a spring, a mounting groove is provided around the through hole on the top of the centrifugal table, the spring is arranged in the mounting groove, a butt ring is further provided in the mounting groove, the butt ring is located above the spring, two ends of the spring are respectively abutted against the butt ring and the wall of the mounting groove, three groups of pressing blocks and three groups of clamping columns are fixedly connected to the top of the butt ring, a fixing strip is provided above the clamping column, the fixing strip is detachably connected to the centrifugal table, the positions of the fixing strip facing the three groups of clamping columns are all provided with through holes, the three groups of clamping columns respectively pass through the three groups of through holes, a locking nut is provided above the fixing strip, the locking nut is threadedly connected to the threaded column and presses against the centrifugal table, a positioning plate is provided on the outer fixed sleeve of the locking nut, the positioning plate has clamping holes at the positions facing the three groups of clamping columns, and the three groups of clamping columns are respectively inserted into the three groups of clamping holes;

[0010] The three groups of pressing blocks are all staggered with the fixing slats.

[0011] As a preferred embodiment of the centrifugal device for separating umbilical cord mesenchymal stem cells described in the present invention, the main body further includes a cover plate and a rubber magnetic strip, the cover plate is hinged to the rear side of the top of the body, the rubber magnetic strip is ring-shaped and fixedly connected to the top of the body, the centrifugal chamber is located inside the rubber magnetic strip, and the cover plate is made of ferromagnetic material and can completely cover the rubber magnetic strip.

[0012] As a preferred solution of the centrifugal device for separating umbilical cord mesenchymal stem cells described in the utility model, the main body also includes a U-shaped handle and a support block, the U-shaped handle is fixedly connected to the front side of the top of the cover plate, and the support blocks are provided in four groups, and the four groups of support blocks are respectively fixedly connected to the four corners of the bottom of the body.

[0013] As a preferred solution of the centrifugal device for separating umbilical cord mesenchymal stem cells described in the present invention, the fixed slat is provided with three groups of mounting holes, and the three groups of mounting holes are equidistantly distributed in a circular shape. The positions of the centrifugal table facing the three groups of mounting holes are provided with screw holes and are equipped with screws. The three groups of screws pass through the three groups of screw holes respectively and are screwed into the corresponding screw holes. The fixed slat is detachably connected to the centrifugal table through the three groups of screws.

[0014] As a preferred embodiment of the centrifuge device for separating umbilical cord mesenchymal stem cells described in the present invention, the number of the centrifuge tube slots is specifically twelve groups, and the twelve groups of centrifuge tube slots are equidistantly distributed in a ring around the axis of the centrifuge table, and the positions on the top of the centrifuge table facing each group of centrifuge tube slots are respectively numbered from one to twelve.

[0015] As a preferred solution of the centrifugal device for separating umbilical cord mesenchymal stem cells described in the utility model, the three groups of positioning columns are equidistantly distributed in a ring, the upper edges of the three groups of positioning columns are chamfered, the three groups of pressing blocks and the three groups of clamping columns are equidistantly distributed in a ring, and the axes of the tray, threaded column, centrifugal table, retaining ring, fixing slat, locking nut and positioning plate are arranged to coincide with each other.

[0016] The beneficial effects of the utility model are as follows: by setting up a connection mechanism, three groups of clamping columns cooperate with the positioning plate to lock the locking nut, so that the locking nut cannot rotate, thereby avoiding over-tightening or loosening of the locking nut after long-term use, and ensuring the stability of the centrifuge. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without inventive work. Among them:

[0018] Figure 1 This is a schematic diagram of the three-dimensional structure of a centrifugal device for separating umbilical cord mesenchymal stem cells in the utility model with its cover in a closed state.

[0019] Figure 2 This is a structural schematic diagram of a centrifugal device for separating umbilical cord mesenchymal stem cells in the utility model with its cover in an open state.

[0020] Figure 3 This is a schematic structural diagram of the centrifugal assembly and drive motor of a centrifugal device for separating umbilical cord mesenchymal stem cells in the utility model.

[0021] Figure 4 This is a structural schematic diagram of the separation state of a tray and a centrifugal component of a centrifugal device for separating umbilical cord mesenchymal stem cells in the utility model.

[0022] Figure 5 This is a schematic diagram of the disassembled structure of the connection mechanism of a centrifugal device for separating umbilical cord mesenchymal stem cells in the utility model.

[0023] Figure 6 This is a partial cross-sectional structural schematic diagram of a centrifugal device for separating umbilical cord mesenchymal stem cells according to the present invention.

[0024] Description of the drawings: 100, main body; 101, machine body; 101a, centrifugal chamber; 102, partition; 103, drive motor; 104, tray; 105, positioning column; 106, threaded column; 107, control panel; 108, cover; 109, rubber magnetic strip; 110, U-shaped handle; 111, support block; 200, centrifugal assembly; 201, centrifugal table; 201a, positioning groove; 201b, through hole; 201c, mounting groove; 300, connecting mechanism; 301, spring; 302, butt ring; 303, pressing block; 304, clamping column; 305, fixing strip; 306, locking nut; 307, positioning plate. DETAILED DESCRIPTION

[0025] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below with reference to the accompanying drawings.

[0026] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0027] Secondly, the term "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in various places throughout this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0028] Furthermore, the present invention is described in detail with reference to schematic diagrams. For ease of illustration, when describing embodiments of the present invention, cross-sectional views of device structures may be partially enlarged and not to scale. Furthermore, the schematic diagrams are merely illustrative and should not limit the scope of protection of the present invention. Furthermore, in actual production, three-dimensional dimensions, including length, width, and depth, should be included.

[0029] Reference Figures 1-6 , which is an embodiment of the present invention, provides a centrifugal device for separating umbilical cord mesenchymal stem cells, comprising:

[0030] The main body 100 includes a body 101. A centrifugal chamber 101a is provided at the top of the body 101. A partition 102 is fixedly connected to the inside of the centrifugal chamber 101a. A drive motor 103 is fixedly connected to the bottom of the partition 102. The shaft of the drive motor 103 passes through the partition 102 and is rotatably connected to the partition 102. A tray 104 is fixedly connected to the top of the shaft of the drive motor 103. Three groups of positioning posts 105 are fixedly connected to the top of the tray 104. A threaded post 106 is fixedly connected to the center of the top of the tray 104. A control panel 107 for controlling the operation of the device is fixedly connected to the front side of the body 101.

[0031] The centrifuge assembly 200 includes a centrifuge table 201 having a plurality of centrifuge tube slots defined on its top. The centrifuge table 201 is disposed above the tray 104. Positions on the centrifuge table 201 facing the three groups of positioning posts 105 each have a positioning slot 201 a defined therein. A through hole 201 b is defined on the centrifuge table 201 facing the threaded post 106. The three groups of positioning posts 105 are respectively inserted into the three groups of positioning slots 201 a, and the threaded post 106 passes through the through hole 201 b.

[0032] The connecting mechanism 300 includes a spring 301. A mounting groove 201c is provided at the top of the centrifugal table 201 around the through hole 201b. The spring 301 is arranged in the mounting groove 201c. A ring 302 is further provided in the mounting groove 201c. The ring 302 is located above the spring 301. The two ends of the spring 301 abut against the ring 302 and the wall of the mounting groove 201c respectively. Three groups of pressing blocks 303 and three groups of clamping columns 304 are fixedly connected to the top of the ring 302. A fixing strip 305 is provided above the clamping column 304. The fixing plate The strip 305 is detachably connected to the centrifugal table 201. Through holes are provided at the positions of the fixed strip 305 facing the three groups of clamping columns 304. The three groups of clamping columns 304 pass through the three groups of through holes respectively. A locking nut 306 is provided above the fixed strip 305. The locking nut 306 is threadedly connected to the threaded column 106 and pressed against the centrifugal table 201. A positioning plate 307 is fixedly sleeved on the outer side of the locking nut 306. The positioning plate 307 is provided with clamping holes at the positions of the positioning plate 307 facing the three groups of clamping columns 304. The three groups of clamping columns 304 are respectively inserted into the three groups of clamping holes.

[0033] Among them, the three groups of pressing blocks 303 are staggered with the fixed strips 305. The main body 100 also includes a cover 108 and a rubber magnetic strip 109. The cover 108 is hinged to the top rear side of the body 101. The rubber magnetic strip 109 is ring-shaped and fixedly connected to the top of the body 101. The centrifugal chamber 101a is located on the inner side of the rubber magnetic strip 109. The cover 108 is made of ferromagnetic material and can completely cover the rubber magnetic strip 109. The cover 108 is used to improve the safety of the device. The magnetic strip 109 is used to adsorb the cover 108 to improve the stability of the cover 108.

[0034] In addition, the main body 100 also includes a U-shaped handle 110 and a support block 111. The U-shaped handle 110 is fixedly connected to the front side of the top of the cover 108. The U-shaped handle 110 is used to facilitate opening the cover 108. There are four groups of support blocks 111. The four groups of support blocks 111 are respectively fixedly connected to the four corners of the bottom of the body 101. The support blocks 111 are used to support the device and improve the stability of the device.

[0035] In addition, three groups of mounting holes are provided on the surface of the fixing slat 305, and the three groups of mounting holes are distributed equidistantly in a circular shape. Screw holes are provided at the positions of the centrifuge table 201 facing the three groups of mounting holes and are equipped with screws. The three groups of screws pass through the three groups of screw holes respectively and are screwed into the corresponding screw holes. The fixing slat 305 is detachably connected to the centrifuge table 201 through the three groups of screws.

[0036] It should be noted that the number of centrifuge tube slots is specifically twelve groups, and the twelve groups of centrifuge tube slots are distributed in a circular and equidistant manner around the axis of the centrifuge table 201. The positions of the top of the centrifuge table 201 opposite to each group of centrifuge tube slots are respectively numbered from one to twelve. The digital numbers are used to facilitate the judgment of whether the placement of the centrifuge tubes is symmetrical. The three groups of positioning columns 105 are distributed in a circular and equidistant manner, and the upper edges of the three groups of positioning columns 105 are all chamfered. The three groups of pressing blocks 303 and the three groups of clamping columns 304 are all distributed in a circular and equidistant manner. The axes of the tray 104, threaded column 106, centrifuge table 201, retaining ring 302, fixing strip 305, locking nut 306 and positioning plate 307 are arranged to coincide with each other to ensure the stability of the device structure.

[0037] Working principle: When installing the centrifugal table 201, insert the centrifugal table 201 into the threaded column 106, and insert the three groups of positioning columns 105 into the three groups of positioning grooves 201a, press two groups of pressing blocks 303, the pressing blocks 303 press down the ring 302, the ring 302 drives the three groups of clamping columns 304 to move down, the spring 301 is compressed, and the locking nut 306 is screwed into the threaded column 106 and tightened until the three groups of card holes in the positioning plate 307 are aligned with the three groups of card columns 304. At this time, the pressing block 303 is released, the spring 301 is reset, and the ring 302 is driven to move up, and the ring 302 drives the three groups of card columns 304 to move up. The three groups of card columns 304 are stuck in the corresponding card holes. At this time, the locking nut 306 cannot rotate, and the installation of the centrifugal table 201 is completed;

[0038] During centrifugation, the centrifuge tube containing umbilical cord mesenchymal stem cells is inserted into the centrifuge tube slot of the centrifuge table 201. If there are multiple groups of centrifuge tubes, the centrifuge tubes are inserted into the appropriate slots according to the number of the centrifuge tube slots (this is a prior art and will not be described in detail) so that the centrifuge tubes are evenly distributed. The cover 108 is closed and the drive motor 103 is started through the control panel 107. The drive motor 103 drives the tray 104 to rotate, the tray 104 drives the centrifuge table 201 to rotate, and the centrifuge table 201 drives each group of centrifuge tubes to rotate to achieve centrifugation. After centrifugation is completed, the cover 108 is opened and the centrifuge tubes are removed to complete the operation.

[0039] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, and all of these should be included in the scope of the claims of the present invention.

Claims

1. A centrifugal device for separating umbilical cord mesenchymal stem cells, characterized in that: include: The main body (100) comprises a body (101), a centrifugal chamber (101a) is provided at the top of the body (101), a partition (102) is fixedly connected to the inside of the centrifugal chamber (101a), a driving motor (103) is fixedly connected to the bottom of the partition (102), a rotating shaft of the driving motor (103) passes through the partition (102) and is rotatably connected to the partition (102), a tray (104) is fixedly connected to the top of the rotating shaft of the driving motor (103), three groups of positioning columns (105) are fixedly connected to the top of the tray (104), a threaded column (106) is fixedly connected to the center of the top of the tray (104), and a control panel (107) for controlling the operation of the device is fixedly connected to the front side of the body (101); A centrifugal assembly (200) comprises a centrifugal table (201), wherein a plurality of centrifuge tube slots are provided on the top of the centrifugal table (201), the centrifugal table (201) is arranged above a tray (104), a positioning groove (201a) is provided on the centrifugal table (201) at positions facing three groups of positioning columns (105), a through hole (201b) is provided on the centrifugal table (201) at positions facing the threaded column (106), the three groups of positioning columns (105) are respectively inserted into the three groups of positioning grooves (201a), and the threaded column (106) passes through the through hole (201b); The connecting mechanism (300) includes a spring (301), a mounting groove (201c) is provided around the through hole (201b) at the top of the centrifugal table (201), the spring (301) is arranged in the mounting groove (201c), a retaining ring (302) is further provided in the mounting groove (201c), the retaining ring (302) is located above the spring (301), the two ends of the spring (301) are respectively in contact with the retaining ring (302) and the groove wall of the mounting groove (201c), three groups of pressing blocks (303) and three groups of clamping columns (304) are fixedly connected to the top of the retaining ring (302), and a fixing strip (305) is provided above the clamping column (304). The fixing strip (305) is detachably connected to the centrifugal table (201), and the fixing strip (305) is provided with through holes at positions facing the three groups of clamping columns (304), and the three groups of clamping columns (304) respectively pass through the three groups of through holes. A locking nut (306) is provided above the fixing strip (305), and the locking nut (306) is threadedly connected to the threaded column (106) and pressed against the centrifugal table (201). A positioning plate (307) is fixedly sleeved on the outer side of the locking nut (306), and the positioning plate (307) is provided with clamping holes at positions facing the three groups of clamping columns (304), and the three groups of clamping columns (304) are respectively inserted into the three groups of clamping holes; The three groups of pressing blocks (303) are all staggered with the fixing strips (305).

2. A centrifugal device for separating umbilical cord mesenchymal stem cells according to claim 1, characterized in that: The main body (100) further comprises a cover plate (108) and a rubber magnetic strip (109), wherein the cover plate (108) is hinged to the rear side of the top of the body (101), the rubber magnetic strip (109) is ring-shaped and fixedly connected to the top of the body (101), the centrifugal chamber (101a) is located inside the rubber magnetic strip (109), and the cover plate (108) is made of ferromagnetic material and can completely cover the rubber magnetic strip (109).

3. A centrifugal device for separating umbilical cord mesenchymal stem cells according to claim 2, characterized in that: The main body (100) further comprises a U-shaped handle (110) and a support block (111), wherein the U-shaped handle (110) is fixedly connected to the front side of the top of the cover plate (108), and the support block (111) is provided in four groups, and the four groups of support blocks (111) are respectively fixedly connected to the four corners of the bottom of the body (101).

4. The centrifugal device for separating umbilical cord mesenchymal stem cells according to claim 1, characterized in that: The fixed slat (305) is provided with three groups of mounting holes, and the three groups of mounting holes are distributed in a circular shape with equal spacing. The centrifugal table (201) is provided with screw holes at positions opposite to the three groups of mounting holes and is equipped with screws. The three groups of screws pass through the three groups of screw holes respectively and are screwed into the corresponding screw holes. The fixed slat (305) is detachably connected to the centrifugal table (201) through the three groups of screws.

5. The centrifugal device for separating umbilical cord mesenchymal stem cells according to claim 1, characterized in that: The number of the centrifuge tube slots is specifically twelve groups, and the twelve groups of centrifuge tube slots are distributed in a circular manner with equal spacing around the axis of the centrifuge table (201), and the positions of the top of the centrifuge table (201) facing each group of centrifuge tube slots are respectively provided with numbers from one to twelve.

6. The centrifugal device for separating umbilical cord mesenchymal stem cells according to claim 1, characterized in that: The three groups of positioning posts (105) are distributed in an annular manner with equal spacing, the upper edges of the three groups of positioning posts (105) are all chamfered, the three groups of pressing blocks (303) and the three groups of clamping posts (304) are all distributed in an annular manner with equal spacing, and the axes of the tray (104), threaded post (106), centrifugal table (201), retaining ring (302), fixing slat (305), locking nut (306) and positioning plate (307) are arranged to coincide with each other.

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