High-temperature-resistant PCR (Polymerase Chain Reaction) material processing device

Abstract

The efficient high-temperature-resistant PCR material processing device comprises a plate cavity main body, a pipe body and a pipe cover, the plate cavity main body is made of borosilicate glass and has good high-temperature resistance, the pipe body and the pipe cover are made of pp materials and can also adapt to the high-temperature environment, a pipe opening is formed in the top of the pipe body, an inserting groove is formed in the outer side of a clamping ring at the top of a placing hole, and the inserting groove is communicated with the pipe opening. The bottom of the pipe cover is provided with a sealing plug, the outer side of the pipe cover is provided with a sealing ring, and the inner side of the pipe opening is provided with a corresponding sealing groove, so that the sealing performance is improved; the mounting plate is internally provided with a distance groove, the pipe cover and a clamping ring, the top of the mounting plate is provided with a pressing part, and the bottom of the mounting plate is provided with a positioning column which is in inserted connection with a positioning hole in the plate hole main body; the spacing grooves in the mounting plate correspond to the placing holes in the plate cavity main body, and the pipe cover is fixedly mounted through the mounting plate, so that the structural design enhances the fixing stability of the pipe body, improves the sealing performance of the device, is convenient to operate, and provides powerful guarantee for efficient and accurate PCR experiments.

Description

Technical Field

[0001] The utility model belongs to the technical field related to molecular biology, and in particular relates to a high-temperature resistant PCR material processing device. Background Art

[0002] PCR, or polymerase chain reaction, is a molecular biology technique used to amplify specific DNA fragments. Its basic principle is to simulate the natural DNA replication process in vitro, denaturing the DNA fragment to be amplified into single strands at high temperatures. These fragments then bind to specific primers at lower temperatures, followed by DNA polymerase-catalyzed synthesis of new DNA strands at an appropriate temperature. This cycle is repeated, exponentially amplifying specific DNA fragments in a short period of time. PCR technology is widely used in medical diagnostic biology research, forensic identification, and other fields.

[0003] However, existing PCR plate wells contain numerous tubes, and sealing these numerous tubes one by one consumes significant experimental preparation time, prolonging the entire experimental process and reducing overall experimental efficiency. This increase in time costs is particularly significant when performing numerous repeated experiments or processing multiple samples. Utility Model Content

[0004] The present invention aims to provide a high-temperature resistant PCR material processing device to address the aforementioned background art issue, which concerns the large number of tubes within existing PCR plate wells. The need to individually seal these numerous tubes consumes significant experimental preparation time, prolonging the entire experimental process and reducing overall experimental efficiency. This significant increase in time costs is particularly pronounced when numerous repeated experiments are required or when processing multiple samples.

[0005] To achieve the above-mentioned purpose, the present utility model provides the following technical solutions: a high-temperature resistant PCR material processing device, comprising a plate well body;

[0006] A base is provided at the bottom of the plate hole body, placement holes are arranged in an array at the inner position of the plate hole body, a mark is provided at the top position of the plate hole body, and a tube body is provided at the inner position of the placement hole;

[0007] A mounting plate is provided at the top of the plate hole body, a spacing groove is provided in an array at an inner position of the mounting plate, and a pipe cover is provided at an inner position of the mounting plate.

[0008] Preferably, a clamping ring is provided at an inner position of the distance groove, and a pressing portion is provided at a top position of the tube cover, and the tube cover fixes the pressing portion through the clamping ring.

[0009] Preferably, a positioning column is provided at the bottom of the mounting plate, and an array of positioning holes is provided inside the plate hole body, and the positioning holes are plug-connected with the positioning column.

[0010] Preferably, the internal spacing groove of the mounting plate corresponds to the internal placement hole of the plate hole body, and the pipe cover is installed and fixed by the mounting plate.

[0011] Preferably, a pipe mouth is provided at the top position of the tube body, a clamping ring is provided at the top position of the placement hole, a plug-in groove is provided at the outer position of the clamping ring, and the tube body is nested and connected with the plug-in groove and the clamping ring through the pipe mouth.

[0012] Preferably, a sealing plug is provided at the bottom of the tube cover, a sealing ring is provided at the outer side of the sealing plug, and a sealing groove corresponding to the sealing ring is provided at the inner side of the tube mouth.

[0013] Preferably, a disassembly portion is provided at the outer side of the tube cover, the plate hole main body is made of borosilicate glass, and the tube body and the tube cover are made of PP material.

[0014] Compared with the prior art, the present invention provides a high-temperature resistant PCR material processing device with the following beneficial effects:

[0015] 1. Through the arrangement of the mounting plate, spacing grooves, tube cover, clamping ring, pressing portion, positioning posts, and positioning holes, the spacing grooves inside the mounting plate cooperate with the tube cover, and the clamping ring inside the spacing grooves secures the pressing portion at the top of the tube cover, allowing the tube cover to tightly cover the tube body. At the same time, the sealing ring outside the sealing plug at the bottom of the tube cover cooperates with the sealing groove inside the tube body, further improving the sealing performance of the device. This multi-sealing structure effectively prevents the ingress of external impurities and the leakage of reaction system substances, ensuring the accuracy of PCR reactions. The integrated mounting plate design can seal multiple tubes at once, greatly improving sealing efficiency and shortening experimental preparation time. This advantage is particularly evident when performing a large number of repeated experiments or processing multiple samples. The positioning posts at the bottom of the mounting plate plug into the positioning holes inside the plate well body to ensure the accurate positioning of the mounting plate. The tube body is nested and connected to the plug-in groove outside the clamping ring at the top of the placement hole inside the plate well body to achieve initial fixation of the tube body. The tube cover is fixed by the mounting plate, making the tube body less likely to shake during operation and improving experimental stability.

[0016] 2. Through the arrangement of the tube mouth, the clamping ring, the plug-in groove, the sealing plug and the sealing ring, the tube body is nested and connected with the plug-in groove outside the clamping ring at the top of the placement hole through the tube mouth. This connection method provides a stable fixing point for the tube body. During the experimental operation, the tube body is not easy to shake or shift, ensuring the accuracy and stability of the experiment. Especially when conducting some experiments requiring precise operation, such as the processing of trace samples, this firm fixing method can avoid sample loss or cross-contamination caused by shaking of the tube body. A sealing ring is provided on the outside of the sealing plug at the bottom of the tube cap, and a sealing groove corresponding to the sealing ring is provided on the inside of the tube mouth. When the tube cap is covered on the tube body, the sealing ring and the sealing groove fit tightly to form a good sealing effect. This sealing structure can effectively prevent external impurities from entering the tube body, ensuring the purity of the PCR reaction system. At the same time, it can also prevent the reaction substances in the tube body from leaking, avoiding pollution to the experimental environment, and improving the reliability and safety of the experiment. This structural design not only enhances the fixed stability of the tube body, but also improves the sealing performance of the device, providing a strong guarantee for efficient and accurate PCR experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a structural diagram of the present utility model.

[0018] Figure 2 This is a structural diagram of the placement of the tube body in the utility model.

[0019] Figure 3 This is a schematic diagram of the structure of installing the pipe cover in the present utility model.

[0020] Figure 4 It is a structural schematic diagram of the mounting plate in the utility model.

[0021] Figure 5 This is a structural diagram of the utility model after the middle pipe cover is installed.

[0022] Figure 6 It is a structural schematic diagram of the PCR tube seal in the present utility model.

[0023] Figure 7 It is a structural schematic diagram of the tube body in the utility model.

[0024] Figure 8 This is a schematic structural diagram of the middle tube cover of the present invention.

[0025] Figure 9 It is a structural schematic diagram of the placement hole in the utility model.

[0026] In the figure: 1. Plate hole body; 2. Base; 3. Placement hole; 4. Positioning hole; 5. Mark; 6. Pipe body; 7. Mounting plate; 8. Pipe cover; 9. Distance groove; 10. Tightening ring; 11. Positioning column; 12. Pressing part; 13. Disassembly part; 14. Sealing groove; 15. Pipe mouth; 16. Sealing plug; 17. Sealing ring; 18. Card slot; 19. Connecting slot. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0028] The utility model provides Figure 1-9 A high temperature resistant PCR material processing device shown includes a plate well body 1;

[0029] A base 2 is provided at the bottom of the plate hole body 1, placement holes 3 are arranged in an array at the inner position of the plate hole body 1, a mark 5 is provided at the top position of the plate hole body 1, and a tube body 6 is provided inside the placement hole 3;

[0030] A mounting plate 7 is provided at the top of the plate hole body 1 , a distance groove 9 is provided in an array at an inner position of the mounting plate 7 , and a pipe cover 8 is provided at an inner position of the mounting plate 7 .

[0031] A clamping ring 10 is provided at an inner position of the spacing groove 9 , and a pressing portion 12 is provided at a top position of the pipe cover 8 . The pipe cover 8 fixes the pressing portion 12 through the clamping ring 10 .

[0032] A positioning column 11 is provided at the bottom of the mounting plate 7 , and an array of positioning holes 4 is provided inside the plate hole body 1 , and the positioning holes 4 are plug-connected with the positioning column 11 .

[0033] The internal spacing groove 9 of the mounting plate 7 corresponds to the internal placement hole 3 of the plate hole body 1 , and the pipe cover 8 is installed and fixed through the mounting plate 7 .

[0034] A pipe opening 15 is provided at the top of the tube body 6 , a clamping ring 18 is provided at the top of the placement hole 3 , and an inserting groove 19 is provided outside the clamping ring 18 . The tube body 6 is nested and connected with the inserting groove 19 and the clamping ring 18 through the pipe opening 15 .

[0035] A sealing plug 16 is provided at the bottom of the pipe cover 8 , a sealing ring 17 is provided outside the sealing plug 16 , and a sealing groove 14 corresponding to the sealing ring 17 is provided inside the pipe opening 15 .

[0036] A disassembly portion 13 is provided at the outer side of the tube cover 8. The plate cavity body 1 is made of borosilicate glass, and the tube body 6 and the tube cover 8 are made of PP material.

[0037] In this embodiment, a specific implementation step of a high-temperature resistant PCR material processing device is provided. The high-efficiency high-temperature resistant PCR material processing device is mainly composed of a plate hole body 1, a base 2, a placement hole 3, a tube body 6, a mounting plate 7, a tube cover 8, etc. The base 2 at the bottom of the plate hole body 1 plays a supporting role, the placement hole 3 inside the plate hole body 1 is used to place the tube body 6, and the mark 5 on the top of the plate hole body 1 can be used to distinguish different samples. The mounting plate 7 is plugged into the positioning hole 4 on the plate hole body 1 through the positioning column 11 at the bottom to ensure that the position of the mounting plate 7 is accurate. The spacing groove 9 inside the mounting plate 7 is used to place the tube cover 8. The clamping ring 10 in the spacing groove 9 can fix the pressing part 12 on the top of the tube cover 8. The pipe mouth 15 at the top of the tube body 6 is nested and connected with the plug-in groove 19 on the outside of the clamping ring 18 at the top of the placement hole 3 to achieve preliminary fixation of the tube body 6. The sealing ring 1 on the outside of the sealing plug 16 at the bottom of the tube cover 8 is connected to the sealing ring 1 7 cooperates with the sealing groove 14 on the inner side of the tube mouth 15 to further improve the sealing performance of the device. The disassembly part 13 on the outside of the tube cover 8 facilitates the disassembly of the tube cover 8. The plate hole main body 1 is made of borosilicate glass and has good high temperature resistance. The tube body 6 and the tube cover 8 are made of PP material, which also has good high temperature resistance. When performing a large number of tube body 6 sealing operations, the integral mounting plate 7 can cover multiple tube bodies 6 at one time, and is quickly connected to the plate hole main body 1 through the positioning column 11, and then the tube cover 8 is fixed with the clamping ring 10, which greatly improves the sealing efficiency and shortens the experimental preparation time. During the PCR experiment, the DNA fragment to be amplified is placed in the tube body 6, and then the tube body 6 is placed in the placement hole 3 of the plate hole main body 1, and the tube cover 8 is covered to ensure good sealing. The device can stably perform PCR reactions during the high temperature, low temperature and suitable temperature cycles to ensure the accuracy of the experimental results.

[0038] like Figure 3-6 As shown, a mounting plate 7 is provided at the top position of the plate hole main body 1, and a distance groove 9 is provided in an array at the internal position of the mounting plate 7. A pipe cover 8 is provided at the internal position of the mounting plate 7, and a tightening ring 10 is provided at the internal position of the distance groove 9. A pressing portion 12 is provided at the top position of the pipe cover 8, and the pipe cover 8 fixes the pressing portion 12 through the tightening ring 10. A positioning column 11 is provided at the bottom position of the mounting plate 7, and a positioning hole 4 is provided in an array inside the plate hole main body 1. The positioning hole 4 is plugged into the positioning column 11. The distance groove 9 inside the mounting plate 7 corresponds to the placement hole 3 inside the plate hole main body 1, and the pipe cover 8 is installed and fixed through the mounting plate 7.

[0039] Preferably, the spacing groove 9 inside the mounting plate 7 cooperates with the tube cover 8, and the tightening ring 10 in the spacing groove 9 fixes the pressing portion 12 on the top of the tube cover 8, so that the tube cover 8 can be tightly covered on the tube body. At the same time, the sealing ring 17 on the outside of the sealing plug 16 at the bottom of the tube cover 8 cooperates with the sealing groove 14 on the inside of the tube mouth 15 of the tube body 6, further improving the sealing performance of the device. This multiple sealing structure can effectively prevent the entry of external impurities and the leakage of reaction system substances, thereby ensuring the accuracy of the PCR reaction. The design of the integral mounting plate 7 can seal multiple tube bodies 6 at one time. The sealing operation greatly improves the sealing efficiency and shortens the experimental preparation time. Especially when a large number of repeated experiments or the processing of multiple samples are required, the advantages are more obvious. The positioning column 11 at the bottom of the mounting plate 7 is plugged into the positioning hole 4 inside the plate hole main body 1 to ensure the accurate position of the mounting plate 7. The tube body 6 is nested and connected with the plug-in groove 19 outside the clamping ring 18 at the top of the hole 3 inside the plate hole main body 1 to achieve the initial fixation of the tube body 6. The tube cover 8 is installed and fixed by the mounting plate 7, so that the tube body 6 is not easy to shake during operation, thereby improving the stability of the experiment.

[0040] like Figure 1 and Figure 7-9 As shown, a pipe mouth 15 is provided at the top position of the tube body 6, a clamping ring 18 is provided at the top position of the placement hole 3, and a plug-in groove 19 is provided at the outer position of the clamping ring 18. The tube body 6 is nested and connected with the plug-in groove 19 and the clamping ring 18 through the pipe mouth 15. A sealing plug 16 is provided at the bottom position of the tube cover 8, a sealing ring 17 is provided at the outer position of the sealing plug 16, and a sealing groove 14 corresponding to the sealing ring 17 is provided at the inner position of the pipe mouth 15.

[0041] Preferably, the tube body 6 is nested and connected with the insertion groove 19 on the outside of the clamping ring 18 at the top of the placement hole 3 through the tube mouth 15. This connection method provides a stable fixing point for the tube body 6. During the experimental operation, the tube body 6 is not easy to shake or shift, ensuring the accuracy and stability of the experiment. Especially when conducting some experiments that require precise operation, such as the processing of trace samples, this firm fixing method can avoid sample loss or cross contamination caused by shaking of the tube body. A sealing ring 17 is provided on the outside of the sealing plug 16 at the bottom of the tube cover 8, and a sealing ring 17 is provided on the inside of the tube mouth 15. The sealing ring 17 corresponds to the sealing groove 14. When the tube cover 8 is covered on the tube body 6, the sealing ring 17 and the sealing groove 14 are tightly matched to form a good sealing effect. This sealing structure can effectively prevent external impurities from entering the tube body 6, ensuring the purity of the PCR reaction system. At the same time, it can also prevent the reaction substances in the tube body 6 from leaking, avoiding pollution to the experimental environment, and improving the reliability and safety of the experiment. This structural design not only enhances the fixed stability of the tube body, but also improves the sealing of the device, providing a strong guarantee for efficient and accurate PCR experiments.

[0042] like Figure 1-2 and Figure 7-8 As shown, a disassembly portion 13 is provided at the outer side of the tube cover 8, the plate cavity body 1 is made of borosilicate glass, and the tube body 6 and the tube cover 8 are made of PP material.

[0043] Optionally, a disassembly portion 13 is provided on the outside of the tube cover 8. When the tube cover 8 needs to be opened, the operator can easily remove the tube cover 8 from the tube body 6 through the disassembly portion 13 without the aid of other tools, thereby improving the convenience of operation. Especially when performing frequent experimental operations, this design can save time and improve work efficiency. The plate hole main body 1 is made of borosilicate glass, and the tube body 6 and the tube cover 8 are made of pp material. Borosilicate glass has good high temperature resistance and can withstand the high temperature environment during the PCR experiment.

[0044] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent replacements for some of the technical features therein. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A high temperature resistant PCR material processing device, comprising a plate well body (1); A base (2) is provided at the bottom of the plate hole body (1), placement holes (3) are arranged in an array at the interior of the plate hole body (1), a mark (5) is provided at the top of the plate hole body (1), and a tube (6) is provided at the interior of the placement hole (3); Its characteristics are: A mounting plate (7) is provided at the top of the plate hole body (1), spacing grooves (9) are arranged in an array at the inner position of the mounting plate (7), and a pipe cover (8) is provided at the inner position of the mounting plate (7).

2. The high temperature resistant PCR material processing device according to claim 1, characterized in that: A clamping ring (10) is provided at an inner position of the spacing groove (9), and a pressing portion (12) is provided at a top position of the tube cover (8), and the tube cover (8) fixes the pressing portion (12) through the clamping ring (10).

3. The high temperature resistant PCR material processing device according to claim 2, characterized in that: A positioning column (11) is provided at the bottom of the mounting plate (7), and an array of positioning holes (4) is provided inside the plate hole body (1), and the positioning holes (4) are plug-connected with the positioning column (11).

4. The high temperature resistant PCR material processing device according to claim 3, characterized in that: The internal spacing groove (9) of the mounting plate (7) corresponds to the internal placement hole (3) of the plate hole body (1), and the pipe cover (8) is installed and fixed through the mounting plate (7).

5. The high temperature resistant PCR material processing device according to claim 1, characterized in that: The tube body (6) is provided with a pipe opening (15) at the top position, the placement hole (3) is provided with a clamping ring (18) at the top position, and an inserting groove (19) is provided at the outer position of the clamping ring (18). The tube body (6) is nested and connected with the inserting groove (19) and the clamping ring (18) through the pipe opening (15).

6. The high temperature resistant PCR material processing device according to claim 5, characterized in that: A sealing plug (16) is provided at the bottom of the pipe cover (8), a sealing ring (17) is provided at the outer side of the sealing plug (16), and a sealing groove (14) corresponding to the sealing ring (17) is provided at the inner side of the pipe opening (15).

7. The high temperature resistant PCR material processing device according to claim 1, characterized in that: A disassembly portion (13) is provided at the outer side of the tube cover (8); the plate hole main body (1) is made of borosilicate glass; and the tube body (6) and the tube cover (8) are made of PP material.

Images