Temperature measuring device and biochemical reaction system

Abstract

The utility model discloses a temperature measuring device and a biochemical reaction system, the temperature measuring device comprises a sleeve, a plurality of temperature sensors and a heat-conducting medium, the sleeve comprises a sleeve body, a first end part and a second end part, the first end part and the second end part are connected to two opposite ends of the sleeve body, and the sleeve body, the first end part and the second end part define a closed cavity; the temperature sensor penetrates through the first end part and extends towards the second end part; the heat-conducting medium is located in the cavity and wraps the temperature sensor. The temperature measuring device can simulate the actual working condition of the fluid pipeline to measure the temperature, and is high in reliability and stability, good in temperature measuring repeatability and high in precision; a plurality of temperature sensors are arranged in the sleeve, so that multi-point testing can be synchronously carried out, and uniform testing of multi-point temperature in the sleeve is realized; and the plurality of temperature sensors and the first end part are prefabricated into a whole in advance, so that disassembly and assembly are convenient.

Description

Technical Field

[0001] The present application relates to the field of temperature detection technology, and in particular to a temperature measuring device and a biochemical reaction system. Background Art

[0002] In medical equipment and biochemical research equipment, most biochemical reactions need to be carried out within fluid pipelines. These pipelines need to be placed on a variable temperature structure that has a certain temperature variation to provide the required temperature for the biochemical reaction. During this period, the fluid pipelines need to be temperature-tested to achieve precise temperature control.

[0003] Currently, the main method for measuring the temperature of these fluid pipelines is to install a temperature probe on the temperature-variable structure, measure the temperature of the temperature-variable structure through the temperature probe, and indirectly reflect the temperature of the fluid pipeline. However, this temperature measurement method has poor accuracy and is difficult to reflect the actual temperature of the fluid pipeline. Utility Model Content

[0004] In order to solve at least one of the above defects, it is necessary to provide a temperature measuring device and a biochemical reaction system.

[0005] In a first aspect, an embodiment of the present application provides a temperature measuring device, which includes: a sleeve, multiple temperature sensors and a heat-conducting medium, the sleeve including a sleeve body, and a first end and a second end connected at opposite ends of the sleeve body, the sleeve body, the first end and the second end forming a closed cavity; the temperature sensor is arranged through the first end and extends toward the second end; the heat-conducting medium is located in the cavity and covers the temperature sensor.

[0006] In some possible embodiments, along the length direction from the first end to the second end, the lengths of the plurality of temperature sensors are different.

[0007] In some possible embodiments, the plurality of temperature sensors are arranged in a stepped manner along the length direction.

[0008] In some possible embodiments, the first end portion is detachably mounted on the sleeve body.

[0009] In some possible embodiments, the first end portion includes an integrally provided connecting portion and a fixing portion, the connecting portion is detachably connected to the sleeve body, the fixing portion has a plurality of through holes, and the temperature sensor is provided through the through holes.

[0010] In some possible embodiments, the connecting portion is threadedly connected to the sleeve body, or the connecting portion is connected to the sleeve body through an interference fit.

[0011] In some possible embodiments, the fixing portion is made of rubber, and the diameter of the through hole is smaller than the diameter of the temperature sensor.

[0012] In some possible embodiments, the heat-conducting medium is liquid; and the sleeve is bendable.

[0013] In some possible embodiments, the end of the temperature sensor located outside the cavity is connected to a data collector; and the temperature sensor is a T-shaped temperature measuring wire.

[0014] In a second aspect, an embodiment of the present application provides a biochemical reaction system, which includes a temperature variable structure, wherein the temperature variable structure has a first channel and a second channel, the first channel is provided with a temperature measuring device as described above, and the second channel is provided with a fluid pipeline, which is used to accommodate the fluid required for the biochemical reaction, wherein the material of the sleeve is the same as the material of the fluid pipeline.

[0015] The temperature measuring device of the embodiment of the present application can simulate the actual working conditions of the fluid pipeline to measure the temperature. The temperature measuring device has high reliability and stability, good temperature measurement repeatability, high accuracy, and avoids other substances from contaminating the temperature variable structure during temperature measurement. There is no need to drill holes in the temperature variable structure to avoid affecting the temperature measurement. Multiple temperature sensors are installed in the sleeve, and multi-point tests can be performed simultaneously, achieving uniform testing of multiple temperature points in the sleeve. Multiple temperature sensors are prefabricated into one piece with the first end in advance, which is convenient for disassembly and assembly. By setting the material of the sleeve to be the same as that of the fluid pipeline, the actual working conditions in the fluid pipeline can be further simulated, and the temperature measurement accuracy can be further improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following is a brief introduction to the drawings required for use in the embodiments of the present application. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0017] Figure 1 Schematic diagram of the structure of a biochemical reaction system according to an embodiment of the present application.

[0018] Figure 2 This is a structural diagram of a temperature measuring device according to an embodiment of the present application.

[0019] Figure 3 for Figure 2 Schematic diagram of part of the structure of the temperature measuring device.

[0020] Figure 4 for Figure 2 Schematic diagram of the structure of the first end.

[0021] Figure 5 This is a top view of a temperature measuring device and a fluid pipeline arranged in a temperature variable structure in one embodiment of the present application.

[0022] Description of main component symbols

[0023]

[0024]

[0025] The following specific implementation methods will further illustrate this application in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0027] It should be noted that when a component is referred to as being "fixed to" or "mounted on" another component, it may be directly on the other component or there may be a central component. When a component is referred to as being "disposed on" another component, it may be directly on the other component or there may be a central component. As used herein, the term "and / or" includes all and any combinations of one or more of the relevant listed items.

[0028] In the field of biochemistry, biochemical reaction systems are often used to analyze and detect biochemical substances. During the analysis and detection process, the biochemical substances need to be heated, and the temperature changes during the heating process need to be measured to accurately control the reaction temperature. Biochemical reaction systems typically include a variable temperature structure for providing a specific temperature for the reaction system, and a temperature probe for detecting the temperature of the variable temperature structure. Currently, there are two main methods for installing temperature probes on the variable temperature structure: one method is to fix the temperature probe to the variable temperature structure with glue, so that the temperature probe is in direct contact with the variable temperature structure; the other method is to drill a hole at a specific location in the variable temperature structure and insert the temperature probe into the hole for temperature detection. However, both of the above temperature measurement methods have some defects. The main defects of the first temperature measurement method are: a) Due to the shape of the variable temperature structure, most probes cannot directly contact the temperature action position; b) Using glue to fix it cannot determine whether the probe is in contact with the measured part or whether the distance is consistent, which will cause large temperature differences between multiple locations and poor temperature measurement accuracy; c) Contamination caused by glue and other substances will affect the subsequent installation of fluid pipelines; d) When glue is affected by external factors, it may cause measurement errors. The main defects of the second temperature measurement method are: a) It is necessary to drill holes in the temperature control position in advance, which damages the equipment and affects the temperature performance of the equipment itself; b) The probe cannot fully contact the heating position when inserted into the drill hole, resulting in temperature errors; c) The probe is different from the actual working conditions after being inserted into the drill hole, and cannot truly reflect the specific temperature of the fluid pipeline.

[0029] To do this, see Figure 1 The present invention provides a biochemical reaction system 1000. For example, the biochemical reaction system 1000 may be a gene sequencing system, but is not limited thereto. The biochemical reaction system 1000 may include a temperature measuring device 100 and a temperature-variable structure 200. The temperature measuring device 100 may be installed in the temperature-variable structure 200 to detect the temperature of the temperature-variable structure 200. Specifically, the temperature-variable structure 200 has a first channel 210. The temperature measuring device 100 is located within the first channel 210 to detect the temperature of the temperature-variable structure 200.

[0030] like Figures 1 to 3As shown, the temperature measuring device 100 includes: a sleeve 1, a plurality of temperature sensors 2 and a heat-conducting medium 3. The sleeve 1 includes a sleeve body 11, and a first end 12 and a second end 13 connected to opposite ends of the sleeve body 11. The sleeve body 11, the first end 12 and the second end 13 form a closed cavity 4. The temperature sensor 2 is arranged through the first end 12 and extends toward the second end 13. The heat-conducting medium 3 is located in the cavity 4 and covers the temperature sensor 2. The temperature measuring device 100 can be installed in the first channel 210. The temperature change on the temperature-variable structure 200 will be transmitted to the temperature sensor 2 through the sleeve 1 and the heat-conducting medium 3. In this way, the temperature sensor 2 will reflect the temperature condition of the temperature-variable structure 200 to achieve temperature measurement. Integrating multiple temperature sensors 2 into a sleeve 1 can improve the accuracy of temperature measurement; moreover, compared with separately setting multiple temperature sensors on the temperature-changing structure 200, the integrated multiple temperature sensors 2 can improve the consistency of temperature measurement; in addition, the temperature measuring device 100 is embedded in the first channel 210 as a whole, avoiding the temperature influence caused by drilling holes in the temperature-changing structure 200, and also avoiding other substances from contaminating the temperature-changing structure 200 during temperature measurement.

[0031] The sleeve 1 can be designed based on the structural form of the first channel 210. For example, the length of the sleeve 1 can be consistent with the length of the first channel 210, so that the temperature measuring device 100 is embedded in the entire first channel 210, thereby achieving temperature measurement of the entire first channel 210. It is understandable that the length of the sleeve 1 can also be shorter than the length of the first channel 210, so that the temperature measuring device 100 is embedded in a portion of the first channel 210 to perform local temperature measurement. In addition, the cross-sectional shape of the sleeve 1 can be designed based on the shape of the first channel 210, so that the sleeve 1 and the first channel 210 have a contoured structure, for example, a circular, rectangular, or irregular shape, etc., so that the sleeve 1 can better fit the inner wall of the first channel 210 and improve the accuracy of temperature measurement.

[0032] In some embodiments, the sleeve 1 is a bendable sleeve. The sleeve 1 is flexible and can be bent into a shape that matches the first channel 210 and then embedded in the first channel 210. For example, the material of the sleeve 1 can be a flexible plastic material or a metal material, etc., which can be selected according to actual needs. Specifically, the material of the sleeve 1 can be plastic, such as a flexible plastic tube, which can better match the shape of the first channel 210. Figures 1 to 3 and Figure 5 As shown, when it is necessary to extend the length of the first channel 210 on the temperature variable structure 200, the first channel 210 needs to be bent and coiled. At this time, the temperature measuring device 100 also needs to be bent and coiled. The soft sleeve 1 is conducive to the bending and coiling of the temperature measuring device 100.

[0033] In some embodiments, the first end portion 12 is detachably mounted on the sleeve body 11, and multiple temperature sensors 2 are mounted on the first end portion 12. The prefabricated structure is then prefabricated into an integral unit. The integrated first end portion 12 and temperature sensor 2 are then integrally mounted on the sleeve body 11, facilitating assembly and disassembly of the temperature sensor 2 and facilitating deployment. This solves the traditional problem of multiple separate temperature sensors being difficult to assemble and disassemble on a temperature-variable structure. Furthermore, the multiple temperature sensors 2 prefabricated into an integral unit offer higher reliability, stability, and repeatability in temperature measurement. Furthermore, this structure avoids the poor temperature measurement consistency associated with the traditional method of separately installing multiple temperature sensors, thereby improving temperature detection consistency.

[0034] like Figure 4 As shown, the first end portion 12 includes an integrally provided connecting portion 14 and a fixing portion 15. The connecting portion 14 is detachably connected to the sleeve body 11. The fixing portion 15 has a plurality of through-holes 16, through which the temperature sensors 2 are disposed. A plurality of temperature sensors 2 are pre-installed in the through-holes 16 of the fixing portion 15, and the temperature sensors 2 are integrated with the first end portion 12 into an integrated structure. Thereafter, the integrated first end portion 12 and temperature sensors 2 are mounted on the sleeve body 11.

[0035] In some embodiments, the connecting portion 14 is detachably connected to the sleeve body 11. Specifically, the connecting portion 14 and the sleeve body 11 can be connected by threads, or the connecting portion 14 and the sleeve body 11 can be connected by interference fit.

[0036] In some embodiments, the fixing portion 15 can be made of rubber, and the diameter of the through hole 16 can be slightly smaller than the diameter of the temperature sensor 2. Thus, after the temperature sensor 2 passes through the through hole 16, the elasticity of the rubber can secure the temperature sensor 2 and achieve a sealing effect. It is understood that in other embodiments, the diameter of the through hole 16 can be slightly larger than the diameter of the temperature sensor 2 to facilitate installation. After the temperature sensor 2 is installed in the through hole 16, the through hole 16 can be sealed and fixed with a sealant.

[0037] like Figure 1 and Figure 2As shown, along the length direction a from the first end 12 to the second end 13, the lengths of the multiple temperature sensors 2 are different. By setting the lengths of the multiple temperature sensors 2 to be different, the temperature at different positions within the entire sleeve 1 can be detected, thereby achieving the detection of the temperature at different positions of the variable temperature structure 200, and synchronous multi-point temperature measurement, improving the accuracy of temperature measurement, and facilitating the detection or evaluation of whether the overall temperature of the variable temperature structure 200 is uniform, and accurately determining the consistency of the temperature at multiple points within the variable temperature structure 200. Specifically, along the length direction a, the multiple temperature sensors 2 are arranged in a stepped manner, that is, along the length direction a of the sleeve 1, the lengths of the multiple temperature sensors 2 increase sequentially, so that the temperature at different length positions of the entire sleeve 1 can be detected, thereby achieving temperature measurement at different positions of the entire first channel 210. This design is particularly suitable for application scenarios where the first channel 210 is relatively long.

[0038] In some embodiments, the temperature sensor 2 can be a thin wire with a relatively large length-to-diameter ratio. For example, the temperature sensor 2 can be a T-shaped temperature measuring wire. By using an extremely thin T-shaped temperature measuring wire as the temperature sensor 2, more temperature sensors 2 can be integrated in a limited space with higher accuracy.

[0039] In some embodiments, the end of the temperature sensor 2 located outside the cavity 4 is connected to a data collector or a Sulingke device to achieve rapid temperature display.

[0040] The heat-conducting medium 3 can be liquid. The sleeve 1 is filled with heat-conducting liquid and both ends are sealed to simulate the actual temperature change with liquid. For example, the heat-conducting medium 3 can be oil or pure water.

[0041] like Figure 1 and Figure 5As shown, in actual application, the temperature-variable structure 200 also has a second channel 220, in which a fluid line 230 is installed. A fluid (e.g., a biological sample and a reagent) is passed into the fluid line 230, so that the fluid can realize a biochemical reaction in the fluid line 230. By providing the first channel 210 and the second channel 220 in the temperature-variable structure 200, the temperature measuring device 100 in the first channel 210 can simulate the actual working conditions of the fluid line 230, thereby achieving accurate temperature measurement at different positions of the temperature-variable structure 200. In some embodiments, the material of the sleeve 1 can be the same as that of the fluid line 230. Using the same material as the fluid line 230 as the temperature conducting medium is consistent with the actual working conditions, which can further improve the accuracy of the temperature measurement of the temperature measuring device 100 and reduce the temperature measurement error. It is understandable that the number of the first channels 210 can be designed according to actual needs and can be one or more. When the first channel 210 is one line, it can be arranged in a curved and coiled form, so that multi-point temperature measurement can be performed on different positions of the temperature-variable structure 200, and the actual temperature condition of the fluid pipeline 230 can be simulated more accurately.

[0042] Taking the biochemical reaction system 1000 as a gene sequencing system as an example, the use of the temperature measuring device 100 is described.

[0043] During use, the temperature measuring device 100 is installed in the corresponding first channel 210 according to the installation method of the actual fluid pipeline 230. That is, the temperature measuring device 100 is installed in the first channel 210 in the same manner as the fluid pipeline 230 is installed in the second channel 220, ensuring that the temperature measuring device 100 can simulate the actual operating conditions of the real fluid pipeline 230 to the greatest extent possible. A data acquisition device connected to the outside of the temperature sensor 2 can process the collected data to obtain relevant parameters. Based on these parameters, the temperature adjustment capacity of the temperature variable structure 200 can be further adjusted.

[0044] It is understood that the temperature measuring device 100 can also be installed in the first channel 210 and the second channel 220 of the temperature variable structure 200, thereby performing temperature testing on all channels of the temperature variable structure 200. It is also understood that the temperature measuring device 100 can be retained on the temperature variable structure 200 for a long time as needed and connected to the control system of the biochemical reaction system 1000 to achieve real-time temperature feedback, facilitating device temperature control. This temperature measuring device 100 can achieve efficient and accurate temperature measurement and can be used stably and long-term in equipment production testing and actual equipment use scenarios. In the embodiment of the present application, the temperature measuring device 100 in the biochemical reaction system 1000 can simulate the actual working conditions of the fluid pipeline 230 for temperature measurement. The temperature measuring device 100 has high reliability and stability, good temperature measurement repeatability, and high accuracy, avoiding other substances from contaminating the temperature variable structure 200 during temperature measurement, and there is no need to drill holes on the temperature variable structure 200 to avoid affecting temperature measurement; multiple temperature sensors 2 are installed in the sleeve 1, and multi-point tests can be performed simultaneously, achieving uniform testing of multiple temperature points in the sleeve 1; multiple temperature sensors 2 are prefabricated into one piece with the first end 12 in advance, which is convenient for disassembly and assembly; by setting the material of the sleeve 1 to be the same as that of the fluid pipeline 230, the actual working conditions in the fluid pipeline 230 can be further simulated, and the temperature measurement accuracy can be further improved.

[0045] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application and are not intended to limit the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present application.

Claims

1. A temperature measuring device, characterized in that: include: The sleeve comprises a sleeve body, and a first end portion and a second end portion connected to opposite ends of the sleeve body, wherein the sleeve body, the first end portion and the second end portion form a closed cavity; a plurality of temperature sensors disposed through the first end portion and extending toward the second end portion; as well as The heat-conducting medium is located in the cavity and covers the temperature sensor.

2. The temperature measuring device according to claim 1, wherein Along a length direction from the first end portion to the second end portion, the plurality of temperature sensors have different lengths.

3. The temperature measuring device according to claim 2, characterized in that Along the length direction, the plurality of temperature sensors are arranged in a stepped manner.

4. The temperature measuring device according to claim 1, wherein The first end portion is detachably mounted on the sleeve body.

5. The temperature measuring device according to claim 1, wherein: The first end portion includes an integrally provided connecting portion and a fixing portion, the connecting portion being detachably connected to the sleeve body, the fixing portion having a plurality of through holes, and the temperature sensor being provided through the through holes.

6. The temperature measuring device according to claim 5, characterized in that The connecting portion is threadedly connected to the sleeve body, or the connecting portion is connected to the sleeve body through interference fit.

7. The temperature measuring device according to claim 5, characterized in that The fixing portion is made of rubber, and the diameter of the through hole is smaller than the diameter of the temperature sensor.

8. The temperature measuring device according to claim 1, wherein: The heat conducting medium is liquid; The sleeve is bendable.

9. The temperature measuring device according to claim 1, wherein: The end of the temperature sensor located outside the cavity is connected to a data collector; The temperature sensor is a T-shaped temperature measuring wire.

10. A biochemical reaction system, characterized in that: The invention comprises a temperature-variable structure, wherein the temperature-variable structure has a first channel and a second channel, wherein the first channel is provided with a temperature measuring device as claimed in any one of claims 1 to 9, and the second channel is provided with a fluid pipeline, wherein the fluid pipeline is used to accommodate the fluid required for the biochemical reaction, wherein the material of the sleeve is the same as that of the fluid pipeline.

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