Three-color fluorescent light path structure and microscope using same
By introducing a tricolor fluorescence optical path structure into the CX33/CX43 model fluorescence microscope, using a three-way excitation block and a uniform light component, the problem of incomplete field of view is solved, and the function of tricolor fluorescence observation is realized to meet customer needs.
Patent Information
- Application Number
- CN202422359001.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-27
- Publication Date
- 2025-08-29
- Estimated Expiration
- 2034-09-27
AI Technical Summary
The existing CX33/CX43 model fluorescence microscope structure has the problem of incomplete field of view when achieving tricolor fluorescence observation, and the monochromatic fluorescence accessories on the market cannot meet customer needs.
A three-color fluorescent optical path structure is designed, using a three-way excitation block and a uniform light assembly, combining three different bands of light sources and switching circuits, and the uniform light assembly is used to achieve uniformity and miniaturization of the light spot, which is suitable for the narrow fluorescence channel reserved by the CX33/CX43 body.
It realizes that the tricolor fluorescence observation function is provided while the original structure remains unchanged, and the light spot is uniform and miniaturized to meet customer needs.
Smart Images
Figure CN223284458U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of microscopes, in particular to a three-color fluorescence light path structure. Background Art
[0002] In fluorescence microscopy, an excitation cube filters specific wavelengths of light from the light source to stimulate fluorescence or phosphorescence in the sample.
[0003] The CX33 / CX43 fluorescence microscope has a unique structural design. Due to the height of the objective turret being extended, adding fluorescence accessories directly between the trinocular head and the microscope body results in an incomplete field of view (referred to as a black edge). The CX33 / CX43 microscope body itself has a narrow fluorescence channel reserved for fluorescence imaging, so most accessories available on the market (including original ones) are made in the form of monochrome fluorescence accessories. However, there is still a large number of customers who require three-color fluorescence observation on the CX33 / CX43. Summary of the Invention
[0004] In order to realize three-color fluorescence on a fluorescence microscope structure of model CX33 / CX43, the utility model provides a three-color fluorescence light path structure.
[0005] The utility model provides a three-color fluorescent light path structure adopting the following technical solutions:
[0006] A three-color fluorescence optical path structure includes a three-color fluorescence switching light source component and a three-way excitation block. The three-way excitation block can pass light in three bands. The light source component includes light sources of three different bands and a switching circuit. The switching circuit switches to the corresponding optical path and illuminates the corresponding light source according to the received light source selection signal.
[0007] The present invention designs the excitation block as a three-way excitation block, mainly with a specially made filter that can pass light of three different wavelength bands. It is combined with a light source component with three optional wavelength bands. The light source component includes three light sources of different wavelength bands and a switching circuit. The user can select one, two, or three light sources. The switching circuit selects the corresponding light path according to the user's selection and drives the corresponding light source to emit light. The present invention can provide three-color fluorescence without changing the space occupied by the excitation block. It is suitable for a narrow fluorescence channel reserved on the CX33 / CX43 body for fluorescence imaging.
[0008] Preferably, the light source includes a green lamp chip, a blue lamp chip and an ultraviolet lamp chip. The chips of the three lamps are spliced into one body, which is convenient for installation and emits light at the same position. The color and properties of the lamp used in the light source can be selected according to actual needs.
[0009] Preferably, the green light chip includes two. Green light is weak, so two green light chips are provided to enhance the intensity of green light.
[0010] Preferably, the three-color fluorescence optical path structure further includes a light homogenization component, which is located between the light source and the three-way excitation block. Light from the illuminated light source sequentially passes through the light homogenization component and the three-way excitation block to reach the rear focal plane of the objective lens. Because the light source includes a green lamp chip, a blue lamp chip, and an ultraviolet lamp chip, and these three lamp chips are spliced together to form an integrated whole, the emitted light cannot be well concentrated. The light homogenization component is used to concentrate light emitted by light sources at different positions onto the center of the filter of the three-way excitation block, making the emitted light spot sufficiently small and uniform.
[0011] Preferably, the light homogenizing assembly includes two convex lenses, which are arranged in parallel with the convex surfaces of the two convex lenses facing the three-way excitation block. The two convex lenses can make the emitted light spot sufficiently small and uniform.
[0012] Preferably, the two convex lenses are respectively a first convex lens and a second convex lens, the first convex lens is a K9 plano-convex lens with a diameter of 25.4 mm, a focal length of 200 mm, an edge thickness of approximately 3 mm, and a center thickness of approximately 3.5 mm, and the second convex lens is a K9 plano-convex lens with a diameter of 25.4 mm, a focal length of 100 mm, an edge thickness of approximately 2 mm, and a center thickness of approximately 3.1 mm.
[0013] The distance between the first convex lens and the second convex lens is 8 mm. The second convex lens is located between the first convex lens and the light outlet. The distance between the second convex lens and the light outlet is 2 mm.
[0014] By selecting and positioning the two convex lenses, the emitted light spot can be made small and uniform enough, and the light spot can be reduced to about 1mm at the exit thread of the objective lens.
[0015] Preferably, the three wavelengths that the three-way excitation block can pass are: ultraviolet, blue and green, and the corresponding wavelengths are 365, 470 and 530 respectively. These three wavelengths are designed according to actual needs and are not limited to these three wavelengths.
[0016] The utility model also provides a microscope, comprising a microscope body, on which the above-mentioned three-color fluorescence light path structure is assembled.
[0017] In summary, the present invention has at least one of the following beneficial technical effects:
[0018] (1) The utility model is a three-color fluorescence optical path structure. By designing the excitation block as a three-way excitation block, a special filter is mainly made. The filter can pass three different bands of light, and is matched with a light source component with three optional bands. The light source component includes three light sources with different bands and a switching circuit. The user can select one light source, two light sources, or three light sources. The switching circuit selects the corresponding light path according to the user's selection and drives the corresponding light source to emit light. The utility model can achieve the three-color fluorescence without changing the space occupied by the excitation block. It is suitable for a narrow fluorescence channel reserved on the CX33 / CX43 body for fluorescence imaging;
[0019] (2) The three-color fluorescence optical path structure of the present invention also includes a light homogenization component, which is located between the light source and the three-way excitation block. The light from the illuminated light source passes through the light homogenization component and the three-way excitation block in sequence to reach the rear focal plane of the objective lens. Since the light source includes a green lamp chip, a blue lamp chip, and an ultraviolet lamp chip, the three lamp chips are spliced into one body, and therefore the emitted light cannot be well concentrated. The light homogenization component is used to make the light emitted by light sources at different positions all concentrate to the center of the filter of the three-way excitation block, so that the emitted light spot is sufficiently small and uniform;
[0020] (3) The microscope of the present invention replaces the single-pass excitation block with a three-pass excitation block on the basis of the original structure, replaces the original monochromatic light source with a three-color light source, and adds a switching circuit of the light source, so as to realize three-color fluorescence observation on the fluorescence microscope structure of model CX33 / CX43. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1 It is a structural schematic diagram of an embodiment of a three-color fluorescent light path structure of the utility model.
[0022] Figure 2 This is a structural diagram of a light source in an embodiment of a three-color fluorescent light path structure of the utility model.
[0023] Figure 3 This is a light path schematic diagram of an embodiment of a three-color fluorescent light path structure of the utility model.
[0024] Figure 4 This is a wavelength diagram of the filter of a three-way excitation block in an embodiment of a three-color fluorescence optical path structure of the present invention.
[0025] Description of reference numerals:
[0026] 1. Light source; 11. Green lamp chip; 12. Blue lamp chip; 13. UV lamp chip; 2. First convex lens; 3. Second convex lens; 4. Local structure of the three-way excitation block; 41. Filter; 42. Light outlet; 5. Back focal plane of the objective lens; 6. Excitation band; 7. Emission band. DETAILED DESCRIPTION
[0027] In order to make the purpose, technical solutions and advantages of this utility model more clear, the following Figure 1-4 And embodiments, the utility model is further described in detail.
[0028] This embodiment provides a three-color fluorescent light path structure, such as Figure 1 and Figure 3 As shown, it includes a light source 1 component for switching three-color fluorescence, a light homogenizing component and a three-way excitation block. The three-way excitation block can pass light of three bands. The light source 1 component includes light sources 1 of three different bands and a switching circuit. The light homogenizing component is located between the light source 1 and the three-way excitation block. The switching circuit switches to the corresponding light path and lights up the corresponding light source 1 according to the received light source 1 selection signal. The light of the lit light source 1 passes through the light homogenizing component and the three-way excitation block in sequence to reach the rear focal plane 5 of the objective lens.
[0029] The present invention designs the excitation block as a three-way excitation block, mainly with a specially made filter 41. The filter 41 can pass light of three different bands, and is combined with a light source 1 component with three optional bands. The light source 1 component includes three light sources 1 of different bands and a switching circuit. The user can select one light source 1, two light sources 1, or three light sources 1. The switching circuit selects the corresponding light path according to the user's selection and drives the corresponding light source 1 to emit light. The present invention can provide three-color fluorescence without changing the occupied space of the excitation block, and is suitable for a narrow fluorescence channel reserved on the CX33 / CX43 body for fluorescence imaging.
[0030] like Figure 2 As shown, since the light source 1 includes a green lamp chip 11, a blue lamp chip 12 and an ultraviolet lamp chip 13, the chips of the three lamps are spliced into one body, therefore, the emitted light cannot be well gathered, and the light uniformity component is used to make the light emitted by the light source 1 at different positions all gathered to the center of the filter 41 of the three-way excitation block, so that the emitted light spot is small and uniform enough, which is convenient for installation and emitting light at the same position.
[0031] As a preferred embodiment, the green lamp chips 11 include two. Green light is weak, so two green lamp chips 11 are provided to enhance the intensity of green light.
[0032] As a preferred embodiment, the light homogenization component includes two convex lenses, which are arranged in parallel with the convex surfaces of the two convex lenses facing the three-way excitation block. The use of two convex lenses can make the emitted light spot sufficiently small and uniform.
[0033] As a preferred embodiment, the two convex lenses are respectively a first convex lens 2 and a second convex lens 3. The first convex lens 2 is a K9 plano-convex lens with a diameter of 25.4 mm, a focal length of 200 mm, an edge thickness of approximately 3 mm, and a center thickness of approximately 3.5 mm. The second convex lens 3 is a K9 plano-convex lens with a diameter of 25.4 mm, a focal length of 100 mm, an edge thickness of approximately 2 mm, and a center thickness of approximately 3.1 mm.
[0034] like Figure 4 As shown, the distance between the first convex lens 2 and the second convex lens 3 is 8 mm, the second convex lens 3 is located between the first convex lens 2 and the light outlet 42, and the distance between the second convex lens 3 and the light outlet 42 is 2 mm.
[0035] By selecting and positioning the two convex lenses, the emitted light spot can be made small and uniform enough, and the light spot can be reduced to about 1mm at the exit thread of the objective lens.
[0036] As a preferred embodiment, the three wavelength bands that the three-way excitation block can pass are: ultraviolet, blue and green, and the corresponding wavelengths are 365, 470 and 530 respectively. These three wavelength bands are designed according to actual needs and are not limited to these three wavelength bands. The excitation band 6 and emission band 7 of the filter of the three-way excitation block are as follows: Figure 4 shown.
[0037] Another embodiment also provides a microscope, which, based on the original structure, replaces the single-pass excitation block with a three-pass excitation block, replaces the original monochromatic light source with a three-color light source, and adds a light source switching circuit, which can realize three-color fluorescence observation on the fluorescence microscope structure of model CX33 / CX43.
[0038] The above are all preferred embodiments of the present invention, and are not intended to limit the scope of protection of the present invention. Therefore, any equivalent changes made based on the structure, shape, and principle of the present invention should be included in the scope of protection of the present invention.
Claims
1. A three-color fluorescent light path structure, characterized by: The invention comprises a light source (1) component capable of switching three-color fluorescence and a three-way excitation block, wherein the three-way excitation block comprises a filter capable of passing light of three wavelength bands, and the light source (1) component comprises light sources (1) of three different wavelength bands and a switching circuit, wherein the switching circuit switches to a corresponding light path and illuminates the corresponding light source (1) according to a received light source (1) selection signal.
2. The three-color fluorescent light path structure according to claim 1, characterized in that: The light source (1) comprises a green lamp chip (11), a blue lamp chip (12) and an ultraviolet lamp chip (13), and the three lamp chips are assembled into one body.
3. The three-color fluorescent light path structure according to claim 2, characterized in that: The green lamp chips (11) include two.
4. The three-color fluorescent light path structure according to claim 2, characterized in that: The three-color fluorescence optical path structure further comprises a light homogenizing component, wherein the light homogenizing component is located between the light source (1) and the three-way excitation block, and light from the illuminated light source (1) passes through the light homogenizing component and the three-way excitation block in sequence to reach the rear focal plane (5) of the objective lens.
5. The three-color fluorescent light path structure according to claim 4, characterized in that: The light homogenizing component includes two convex lenses, which are arranged in parallel, and the convex surfaces of the two convex lenses are both facing the three-way excitation block.
6. The three-color fluorescent light path structure according to claim 5, characterized in that: The two convex lenses are respectively a first convex lens (2) and a second convex lens (3); the first convex lens (2) is a K9 plano-convex lens with a diameter of 25.4 mm and a focal length of 200 mm; the second convex lens (3) is a K9 plano-convex lens with a diameter of 25.4 mm and a focal length of 100 mm.
7. The three-color fluorescent light path structure according to claim 6, characterized in that: The distance between the first convex lens (2) and the second convex lens (3) is 8 mm, the second convex lens (3) is located between the first convex lens (2) and the light outlet (42), and the distance between the second convex lens (3) and the light outlet (42) is 2 mm.
8. The three-color fluorescent light path structure according to claim 1, characterized in that: The three wavelength bands that the triple-pass excitation block can pass are: ultraviolet, blue and green, and the corresponding wavelengths are 365, 470 and 530 respectively.
9. A microscope, comprising a microscope body, characterized in that: The microscope body is equipped with a three-color fluorescence optical path structure according to any one of claims 1 to 8.