Fluorescent quantitative PCR instrument

Abstract

The embodiment of the utility model provides a fluorescent quantitative PCR (Polymerase Chain Reaction) instrument and relates to the technical field of fluorescent detection equipment. The fluorescent quantitative PCR instrument comprises an instrument shell, a fluorescence detection subsystem and a metal bath subsystem, the fluorescence detection subsystem comprises a light source, a light filtering module, a rotary driving device, a reflection module and an imaging module, and the imaging module, the light filtering module and the imaging module are sequentially arranged; the filtering module is provided with a plurality of annularly arranged filtering channels, and the rotary driving device is connected with the filtering module; the metal bath subsystem comprises a heating module and a linear driving device, the rotary driving device is used for driving the light filtering module to rotate so as to switch different light filtering channels and transmit visible light emitted by the light source to the reflection module, and the reflection module is used for reflecting the visible light to a PCR plate placed on the heating module; and the fluorescent light generated in the holes of the PCR plate is reflected to the light filtering channel and is transmitted to the imaging module. The fluorescent quantitative PCR instrument is small in size and high in space utilization rate.

Description

Technical Field

[0001] The utility model relates to the technical field of fluorescence detection equipment, in particular to a fluorescence quantitative PCR instrument. Background Art

[0002] A fluorescence quantitative PCR instrument is an instrument that uses fluorescent dyes or fluorescently labeled specific probes to track PCR products and monitor the reaction process in real time. Currently, fluorescence quantitative PCR instruments have been widely used in gene expression research, pathogen detection, and genome copy number variation analysis. By adding fluorescent groups to the PCR reaction system, the instrument activates and detects the fluorescent signal to monitor the entire PCR process in real time. Combined with appropriate software, the products can be qualitatively and quantitatively analyzed, accurately measuring the expression level of the target gene or DNA content.

[0003] However, the existing high-throughput fluorescence quantitative PCR instrument has a complex structure and a large size. How to optimize the structural design of the fluorescence quantitative PCR instrument, improve space utilization, and effectively realize the miniaturization of the equipment has become one of the technical problems that need to be solved urgently in the existing technology. Utility Model Content

[0004] The purpose of the utility model is to provide a fluorescence quantitative PCR instrument, which can reduce the volume and improve the space utilization.

[0005] The embodiment of the present utility model is achieved as follows:

[0006] In a first aspect, the present invention provides a fluorescence quantitative PCR instrument, comprising:

[0007] Instrument housing;

[0008] A fluorescence detection subsystem comprising a light source, a filter module, a rotation drive device, a reflection module, and an imaging module, all of which are disposed within the instrument housing, wherein the imaging module, the filter module, and the imaging module are sequentially arranged; the filter module has a plurality of filter channels, which are arranged in a ring; and the rotation drive device is connected to the filter module;

[0009] a metal bath subsystem, the metal bath subsystem comprising a heating module and a linear drive device both disposed within the instrument housing, the heating module being disposed within the linear drive device, the linear drive device being configured to drive the heating module to move horizontally relative to the instrument housing;

[0010] The light source is used to emit visible light toward the filter module, and the rotation drive device is used to drive the filter module to rotate to switch between different filter channels and transmit the visible light to the reflection module. The reflection module is used to reflect the visible light to the PCR plate placed on the heating module, and reflect the fluorescence generated in the PCR plate hole to the filter channel. The fluorescence is transmitted to the imaging module via the filter channel.

[0011] In an optional embodiment, the filter module includes a turntable and a plurality of filter structures, the plurality of filter structures are arranged in an annular manner on the turntable, and each of the filter structures is provided with the filter channel; the turntable is connected to the rotation drive device.

[0012] In an optional embodiment, the number of the filter structures is eight, and the angle between two adjacent filter channels is 45°.

[0013] In an optional embodiment, the rotation driving device includes a first motor and a transmission mechanism, the first motor is in transmission connection with the transmission mechanism, and the transmission mechanism is connected to the filter module.

[0014] In an optional embodiment, the transmission mechanism is a worm gear mechanism.

[0015] In an optional embodiment, the reflection module and the heating module are spaced apart, and the light source is disposed on a side of the filter module close to the heating module.

[0016] In an optional embodiment, the linear drive device includes a second motor, a first synchronous belt, a linear guide and two first synchronous wheels, the two first synchronous wheels are arranged at intervals, the second motor is connected to one of the first synchronous wheels, the first synchronous belt is simultaneously connected to the two first synchronous wheels, the linear guide is arranged on the first synchronous belt, and the heating module is arranged on the linear guide.

[0017] In an optional embodiment, the heating module includes a heating body and a heating mounting frame, the heating body is provided with a plurality of positioning holes, the heating mounting frame is provided with a plurality of positioning pins, and the plurality of positioning holes correspond to the plurality of positioning pins one by one; the heating mounting frame is connected to the linear drive device, and the heating body is used to place the PCR plate.

[0018] In an optional embodiment, the metal bath subsystem further includes a thermal cover module disposed within the instrument housing.

[0019] In an optional embodiment, the thermal cover module includes a thermal cover heating membrane structure and a thermal cover body, and the thermal cover heating membrane structure is detachably connected to the thermal cover body.

[0020] In an optional embodiment, the thermal cover body includes a first fixed structure, a second fixed structure and a lifting structure, the first fixed structure, the lifting structure and the second fixed structure are arranged in sequence, and the thermal cover heating film structure is detachably connected to a side of the lifting structure close to the second fixed structure;

[0021] The thermal cover module further includes a lifting drive device, which is disposed on the lifting structure and is connected to the first fixed structure and the second fixed structure at the same time, so as to drive the lifting structure to rise and fall relative to the first fixed structure and the second fixed structure.

[0022] In an optional embodiment, the lifting drive device includes a third motor, a second synchronous belt, multiple second synchronous wheels and multiple screw rods. The third motor is arranged on the lifting structure and is transmission-connected to the second synchronous belt. The multiple second synchronous wheels are arranged at intervals on the lifting structure; the second synchronous belt is transmission-connected to the multiple second synchronous wheels, and the multiple screw rods are respectively transmission-connected to the multiple second synchronous wheels, and are simultaneously connected to the first fixed structure and the second fixed structure.

[0023] The beneficial effects of the embodiments of the present utility model include:

[0024] The fluorescence quantitative PCR instrument includes an instrument housing, a fluorescence detection subsystem and a metal bath subsystem. The fluorescence detection subsystem includes a light source, a filter module, a rotation drive device, a reflection module and an imaging module, all of which are arranged in the instrument housing. The imaging module, the filter module and the imaging module are arranged in sequence; the filter module has multiple filter channels, the multiple filter channels are arranged in a ring, and the rotation drive device is connected to the filter module; the metal bath subsystem includes a heating module and a linear drive device, both of which are arranged in the instrument housing. The heating module is arranged in the linear drive device, and the linear drive device is used to drive the heating module to move horizontally relative to the instrument housing; wherein, the light source is used to emit visible light toward the filter module, the rotation drive device is used to drive the filter module to rotate to switch different filter channels, and transmit the visible light to the reflection module, the reflection module is used to reflect the visible light to the PCR plate placed on the heating module, and reflect the fluorescence generated in the PCR plate holes to the filter channels, and the fluorescence is transmitted to the imaging module via the filter channels.

[0025] The visible light emitted by the light source needs to be converted into light of the required wavelength by the filter module. This embodiment saves internal space of the fluorescence detection subsystem by arranging multiple filter channels in a ring and using a rotary drive device to switch between different filter channels. In addition, the linear drive device drives the heating module to move horizontally, which makes it easy to push the heating module out of the instrument housing and place the PCR plate on the heating module, making the metal bath subsystem compact, thereby improving the internal space utilization of the fluorescence quantitative PCR instrument and miniaturizing the overall size of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following is a brief introduction to the drawings required for use in the embodiments. It should be understood that the following drawings only illustrate certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying any creative work.

[0027] Figure 1 An exploded view of the fluorescent quantitative PCR instrument provided in an embodiment of the present utility model;

[0028] Figure 2 A schematic structural diagram of a fluorescence detection subsystem provided in an embodiment of the present utility model;

[0029] Figure 3 A schematic structural diagram of a metal bath subsystem provided in an embodiment of the present utility model;

[0030] Figure 4 A schematic diagram of the optical path of a fluorescent quantitative PCR instrument provided in an embodiment of the present invention;

[0031] Figure 5 A schematic diagram of the coordination between the fluorescence detection subsystem and the metal bath subsystem provided in an embodiment of the present utility model;

[0032] Figure 6 An exploded view of the assembly of the filter module and the rotary drive device provided in an embodiment of the present utility model;

[0033] Figure 7 A schematic diagram of the assembly of the linear drive device and the heating module provided in an embodiment of the present utility model;

[0034] Figure 8 A schematic structural diagram of a heating body provided in an embodiment of the present utility model;

[0035] Figure 9 An exploded view of a thermal cover module provided in an embodiment of the present utility model;

[0036] Figure 10A schematic structural diagram of the first lifting state of the thermal cover module provided by an embodiment of the present utility model;

[0037] Figure 11 This is a structural schematic diagram of the second lifting state of the thermal cover module provided by an embodiment of the present utility model.

[0038] Icons: 100-fluorescence quantitative PCR instrument; 10-instrument housing; 20-fluorescence detection subsystem; 21-light source; 22-filter module; 221-filter channel; 222-turntable; 223-filter structure; 23-rotation drive device; 231-first motor; 232-transmission mechanism; 24-reflection module; 241-reflector; 242-plano-convex lens; 25-imaging module; 30-metal bath subsystem; 32-heating module; 321-heating body; 3211-positioning hole; 322-heating mounting bracket ;3221-locating pin;33-linear drive device;331-second motor;332-first synchronous belt;333-linear guide rail;334-first synchronous wheel;34-thermal cover module;341-thermal cover heating film structure;342-thermal cover body;3421-first fixed structure;3422-second fixed structure;3423-lifting structure;343-lifting drive device;3431-third motor;3432-second synchronous belt;3433-second synchronous wheel;3434-screw;200-PCR plate. DETAILED DESCRIPTION

[0039] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings herein can be arranged and designed in various different configurations.

[0040] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but rather merely represents selected embodiments of the present invention. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without creative effort are also within the scope of protection of the present invention.

[0041] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not require further definition or explanation in subsequent drawings.

[0042] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer" and the like, indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, or are the orientations or positional relationships in which the utility model product is typically placed when in use. These terms are intended solely to facilitate the description of this utility model and to simplify the description, and are not intended to indicate or imply that the device or component referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first," "second," and "third," etc., are used solely to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0043] Furthermore, terms such as "horizontal" and "vertical" do not necessarily mean that a component must be absolutely horizontal or overhanging, but rather that it can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal than "vertical," and does not mean that the structure must be completely horizontal, but rather that it can be slightly tilted.

[0044] It should also be noted that, in the description of this utility model, unless otherwise expressly specified or limited, the terms "disposed," "installed," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections; direct connections, indirect connections through an intermediate medium, and internal connections between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on the specific circumstances.

[0045] As described in the background technology, a fluorescence quantitative PCR instrument refers to an instrument that uses fluorescent dyes or fluorescently labeled specific probes to mark and track PCR products and monitor the reaction process in real time. In the prior art, fluorescence quantitative PCR instruments have been widely used in the fields of gene expression research, pathogen detection, and genome copy number variation analysis. By adding fluorescent groups to the PCR reaction system, the fluorescence quantitative PCR instrument is used to activate and detect the fluorescent signal to monitor the entire PCR process in real time. Combined with the corresponding software, the product can be qualitatively and quantitatively analyzed to accurately measure the expression level or DNA content of the target gene. However, the existing high-throughput fluorescence quantitative PCR instrument has a complex structure and a large volume. How to optimize the structural design of the fluorescence quantitative PCR instrument, improve space utilization, and effectively achieve equipment miniaturization has become one of the technical problems that need to be solved in the prior art.

[0046] Based on this, please refer to Figures 1-11 The embodiment of the present invention provides a fluorescence quantitative PCR instrument 100 that can effectively improve the above-mentioned technical problems, that is, it can reduce the volume and improve space utilization. The fluorescence quantitative PCR instrument 100 will be described in detail below.

[0047] Please refer to Figure 1 , Figure 1 The exploded diagram of the fluorescence quantitative PCR instrument 100 provided in this embodiment is combined with Figure 1 The fluorescence quantitative PCR instrument 100 includes an instrument housing 10 , a fluorescence detection subsystem 20 and a metal bath subsystem 30 , and both the fluorescence detection subsystem 20 and the metal bath subsystem 30 are disposed in the instrument housing 10 .

[0048] Specifically, please refer to Figure 2 and Figure 3 , Figure 2 This is a schematic diagram of the structure of the fluorescence detection subsystem 20 provided in this embodiment. Figure 3 The schematic diagram of the structure of the metal bath subsystem 30 provided in this embodiment, combined with Figure 1-Figure 3 The fluorescence detection subsystem 20 includes a light source 21, a filter module 22, a rotation drive device 23, a reflection module 24 and an imaging module 25, all of which are arranged in the instrument housing 10 ( Figure 4 The imaging module 25, the filter module 22, and the imaging module 25 are arranged in sequence; the filter module 22 has a plurality of filter channels 221 arranged in a ring, and the rotary drive device 23 is connected to the filter module 22; the metal bath subsystem 30 includes a heating module 32 and a linear drive device 33, both of which are disposed in the instrument housing 10. The heating module 32 is disposed in the linear drive device 33, and the linear drive device 33 is used to drive the heating module 32 to move horizontally relative to the instrument housing 10; wherein the light source 21 is used to emit visible light toward the filter module 22, and the rotary drive device 23 is used to drive the filter module 22 to rotate to switch between different filter channels 221 and transmit the visible light to the reflection module 24. The reflection module 24 is used to reflect the visible light to the PCR plate 200 placed on the heating module 32, and reflect the fluorescence generated in the wells of the PCR plate 200 to the filter channels 221. The fluorescence is transmitted to the imaging module 25 via the filter channels 221.

[0049] It should be noted that the visible light emitted by the light source 21 needs to be converted into light of the required wavelength by the filter module 22. In this embodiment, the internal space of the fluorescence detection subsystem 20 can be saved by arranging multiple filter channels 221 in a ring and using the rotary drive device 23 to switch different filter channels 221. Moreover, by driving the heating module 32 to move horizontally through the linear drive device 33, the heating module 32 can be easily pushed out of the instrument housing 10 and the PCR plate 200 can be placed on the heating module 32, making the metal bath subsystem 30 compact, thereby improving the internal space utilization of the fluorescence quantitative PCR instrument 100 and realizing the miniaturization of the overall volume of the equipment.

[0050] Please refer to Figure 4 , Figure 4 This is a schematic diagram of the optical path of the fluorescent quantitative PCR instrument 100 provided in this embodiment. Figure 4 Two sets of optical path transmission routes are shown in the figure. The solid arrows represent the optical path of the visible light emitted by the light source 21: the visible light is affected by the filter channel 221 of the filter module 22 to generate light of the required wavelength, and is reflected by the reflection module 24 and transmitted to the PCR plate 200; the dotted arrows represent the optical path of the fluorescence generated in the hole of the PCR plate 200: the fluorescence is reflected by the reflection module 24, and then received by the imaging module 25 through the filter channel 221, thereby completing the fluorescence detection.

[0051] For further information, please refer to Figure 5 , Figure 5 The schematic diagram of the cooperation between the fluorescence detection subsystem 20 and the metal bath subsystem 30 provided in this embodiment is shown in FIG. Figure 4 and Figure 5 The reflection module 24 includes a plano-convex lens 242 and a plurality of reflectors 241. When the heating module 32 is driven to the target position in the instrument housing 10, the plano-convex lens 242 is arranged opposite to the PCR plate 200. The arrangement of the plurality of reflectors 241 can reflect the visible light multiple times, and then transmit it to the PCR plate 200 through the plano-convex lens 242; similarly, the fluorescence generated in the hole of the PCR plate 200 passes through the plano-convex lens 242 and is transmitted to the filter channel 221 through the multiple reflections of the plurality of reflectors 241.

[0052] In this embodiment, the reflection module 24 and the heating module 32 are spaced apart, and the light source 21 is disposed on a side of the filter module 22 close to the heating module 32. Figure 5 As shown, by positioning the light source 21 below the filter module 22, near the side of the heating module 32, the spacing between the overall structure of the fluorescence detection subsystem 20 and the overall structure of the metal bath subsystem 30 can be increased. On the one hand, the distance between the plano-convex lens 242 and the PCR plate 200 is increased, so that the light beam emitted by the light source 21 passing through the plano-convex lens 242 and reaching the PCR plate 200 is a parallel beam, thereby achieving a uniform light intensity distribution for each sample well of the PCR plate 200 and avoiding the generation of abnormal light spots on the surface of the PCR plate 200 due to uneven light intensity distribution. On the other hand, the total optical path length of the fluorescence beam generated in the sample well of the PCR plate 200 to reach the imaging module 25 is increased, thereby reducing the spectral shift of the edge field of view at the filter module 22 and the influence of beam interference. This ensures that the fluorescence generated in each sample well of the PCR plate 200 reaches the imaging module 25 with similar energy, thus avoiding the generation of abnormal light spots in the image obtained by the imaging module 25.

[0053] Please refer to Figure 6 , Figure 6The exploded view of the assembly of the filter module 22 and the rotary drive device 23 provided in this embodiment, combined with Figure 2 and Figure 6 Specifically, the filter module 22 includes a turntable 222 and a plurality of filter structures 223 . The plurality of filter structures 223 are arranged in an annular manner on the turntable 222 , and each filter structure 223 is provided with a filter channel 221 . The turntable 222 is connected to the rotation drive device 23 .

[0054] It should be noted that in this embodiment, the number of filter structures 223 is eight, and the angle between two adjacent filter channels 221 is 45°. Of course, in other embodiments, the number of filter structures 223 may also be four, five, or six, for example. For example, in some other implementations, if the number of filter structures 223 is six, the angle between two adjacent filter channels 221 is 60°.

[0055] Furthermore, the rotation drive device 23 includes a first motor 231 and a transmission mechanism 232. The first motor 231 is in transmission connection with the transmission mechanism 232, and the transmission mechanism 232 is connected to the filter module 22. As will be readily understood, the first motor 231 drives the transmission mechanism 232 to rotate, thereby causing the filter module 22 to rotate, thereby achieving switching between the multiple filter channels 221.

[0056] It should be noted that, in this embodiment, the transmission mechanism 232 is a worm gear mechanism; of course, in other embodiments, the transmission mechanism 232 may also be other transmission modes such as gear transmission or synchronous wheel transmission.

[0057] Please refer to Figure 7 , Figure 7 The assembly diagram of the linear drive device 33 and the heating module 32 provided in this embodiment is combined with Figure 7 Specifically, the linear drive device 33 includes a second motor 331, a first synchronous belt 332, a linear guide 333 and two first synchronous wheels 334. The two first synchronous wheels 334 are arranged at intervals. The second motor 331 is connected to one of the first synchronous wheels 334 for transmission. The first synchronous belt 332 is connected to the two first synchronous wheels 334 for transmission at the same time. The linear guide 333 is arranged on the first synchronous belt 332, and the heating module 32 is arranged on the linear guide 333.

[0058] It is easy to understand that the second motor 331 drives the first synchronous wheel 334 to rotate, thereby driving the first synchronous belt 332 and the linear guide rail 333 to move, thereby enabling the heating module 32 to extend out of the instrument housing 10 or be retracted into the instrument housing 10.

[0059] Furthermore, the heating module 32 includes a heating body 321 and a heating mounting frame 322, please refer to Figure 8 , Figure 8 The schematic diagram of the structure of the heating body 321 provided in this embodiment, combined with Figure 7 and Figure 8 The heating body 321 is provided with a plurality of positioning holes 3211, and the heating mounting bracket 322 is provided with a plurality of positioning pins 3221. The positioning holes 3211 correspond to the positioning pins 3221 in a one-to-one manner. The heating mounting bracket 322 is connected to the linear drive device 33, and the heating body 321 is provided with a receiving position for placing the PCR plate 200. The cooperation between the positioning holes 3211 and the positioning pins 3221 facilitates the installation and removal of the heating body 321, thereby enabling the fluorescence quantitative PCR instrument 100 to adapt to PCR plates 200 of different specifications.

[0060] Furthermore, if Figure 3 As shown, the metal bath subsystem 30 further includes a heat cover module 34 disposed in the instrument housing 10, for preventing the evaporated water vapor of the reaction solution in the sample wells of the PCR plate 200 from condensing on the sealing film on the surface of the PCR plate 200. Figure 9 , Figure 9 The exploded view of the thermal cover module 34 provided in this embodiment is combined with Figure 3 and Figure 9 The thermal cover module 34 includes a thermal cover heating membrane structure 341 and a thermal cover body 342. The thermal cover heating membrane structure 341 is detachably connected to the thermal cover body 342. When the thermal cover module 34 is working, the PCR plate 200 is located between the thermal cover heating membrane structure 341 and the heating module 32, and the thermal cover body 342 is located on the side of the thermal cover heating membrane structure 341 away from the heating module 32.

[0061] Specifically, the thermal cover heating membrane structure 341 is provided with a plurality of through holes, the central axis of each through hole coinciding with the central axis of the sample well at the corresponding position in the PCR plate 200, thereby allowing the visible light emitted by the light source 21 and the fluorescence generated in the sample well to pass through the through hole. At the same time, the detachable connection between the thermal cover body 342 and the thermal cover heating membrane structure 341 facilitates the replacement of the thermal cover heating membrane structure 341 to accommodate PCR plates 200 of different specifications. It should be noted that the detachable connection method may be a bolt connection, a snap connection, a magnetic connection, or other detachable connection methods, which are not limited in this embodiment.

[0062] In order to further save space and improve space utilization, in this embodiment, the heat cover module 34 is configured as a liftable structure. For details, please refer to Figure 10 and Figure 11 , Figure 10 This is a structural diagram of the first lifting state of the thermal cover module 34 provided in this embodiment. Figure 11 The schematic diagram of the second lifting state of the heat cover module 34 provided in this embodiment, combined with Figure 10 and Figure 11 The heat cover body 342 includes a first fixed structure 3421, a second fixed structure 3422 and a lifting structure 3423. The first fixed structure 3421, the lifting structure 3423 and the second fixed structure 3422 are arranged in sequence. The heat cover heating film structure 341 is detachably connected to the lifting structure 3423 on a side close to the second fixed structure 3422. The heat cover module 34 also includes a lifting drive device 343. The lifting drive device 343 is arranged on the lifting structure 3423 and is connected to the first fixed structure 3421 and the second fixed structure 3422 at the same time, so as to drive the lifting structure 3423 to move up and down relative to the first fixed structure 3421 and the second fixed structure 3422.

[0063] Specifically, the lifting drive device 343 includes a third motor 3431, a second synchronous belt 3432, multiple second synchronous wheels 3433 and multiple screw rods 3434. The third motor 3431 is arranged on the lifting structure 3423 and is transmission-connected to the second synchronous belt 3432. The multiple second synchronous wheels 3433 are arranged at intervals on the lifting structure 3423; the second synchronous belt 3432 is transmission-connected to the multiple second synchronous wheels 3433, and the multiple screw rods 3434 are respectively transmission-connected to the multiple second synchronous wheels 3433, and are simultaneously connected to the first fixed structure 3421 and the second fixed structure 3422.

[0064] It can be understood that the third motor 3431 can simultaneously drive multiple second synchronous wheels 3433 to rotate by driving the synchronous belt to move, thereby simultaneously driving multiple screw rods 3434 to rotate simultaneously, so that the lifting structure 3423 can be lifted and lowered relative to the first fixed structure 3421 and the second fixed structure 3422.

[0065] It should be noted that, in this embodiment, the screw rod 3434 is specifically a T-shaped screw rod. Of course, in other embodiments, the screw rod 3434 may also be other structural forms such as a ball screw.

[0066] In summary, the embodiment of the present invention provides a fluorescence quantitative PCR instrument 100, which includes an instrument housing 10, a fluorescence detection subsystem 20, and a metal bath subsystem 30. The fluorescence detection subsystem 20 includes a light source 21, a filter module 22, a rotation drive device 23, a reflection module 24, and an imaging module 25, all of which are arranged in the instrument housing 10. The imaging module 25, the filter module 22, and the imaging module 25 are arranged in sequence; the filter module 22 has a plurality of filter channels 221, and the plurality of filter channels 221 are arranged in a ring. The rotation drive device 23 is connected to the filter module 22; the metal bath subsystem 30 includes a light source 21, a filter module 22, a rotation drive device 23, a reflection module 24, and an imaging module 25, all of which are arranged in the instrument housing 10. The housing 10 includes a heating module 32 and a linear drive device 33. The heating module 32 is arranged on the linear drive device 33. The linear drive device 33 is used to drive the heating module 32 to move horizontally relative to the instrument housing 10. The light source 21 is used to emit visible light toward the filter module 22. The rotation drive device 23 is used to drive the filter module 22 to rotate to switch between different filter channels 221 and transmit the visible light to the reflection module 24. The reflection module 24 is used to reflect the visible light to the PCR plate 200 placed on the heating module 32, and reflect the fluorescence generated in the holes of the PCR plate 200 to the filter channel 221. The fluorescence is transmitted to the imaging module 25 via the filter channel 221.

[0067] That is to say, the visible light emitted by the light source 21 needs to be converted into light of the required wavelength by the filter module 22. In this embodiment, the internal space of the fluorescence detection subsystem 20 can be saved by arranging multiple filter channels 221 in a ring and using the rotation drive device 23 to switch different filter channels 221. Moreover, by driving the heating module 32 to move horizontally through the linear drive device 33, the heating module 32 can be easily pushed out of the instrument housing 10 and the PCR plate 200 can be placed on the heating module 32, making the metal bath subsystem 30 compact, thereby improving the internal space utilization of the fluorescence quantitative PCR instrument 100 and realizing the miniaturization of the overall volume of the equipment.

[0068] The above description is merely a specific embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that the present invention is susceptible to various modifications and variations. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A fluorescence quantitative PCR instrument, characterized in that: include: Instrument housing (10); A fluorescence detection subsystem (20), the fluorescence detection subsystem (20) comprising a light source (21), a filter module (22), a rotation drive device (23), a reflection module (24) and an imaging module (25), all of which are arranged in the instrument housing (10), the imaging module (25), the filter module (22) and the imaging module (25) being arranged in sequence; the filter module (22) having a plurality of filter channels (221), the plurality of filter channels (221) being arranged in a ring shape, and the rotation drive device (23) being connected to the filter module (22); a metal bath subsystem (30), the metal bath subsystem (30) comprising a heating module (32) and a linear drive device (33) both disposed within the instrument housing (10); the heating module (32) being disposed within the linear drive device (33); and the linear drive device (33) being used to drive the heating module (32) to move horizontally relative to the instrument housing (10); The light source (21) is used to emit visible light toward the filter module (22); the rotation drive device (23) is used to drive the filter module (22) to rotate so as to switch between different filter channels (221) and transmit the visible light to the reflection module (24); the reflection module (24) is used to reflect the visible light to the PCR plate (200) placed on the heating module (32) and reflect the fluorescence generated in the hole of the PCR plate (200) to the filter channel (221); and the fluorescence is transmitted to the imaging module (25) via the filter channel (221).

2. The fluorescence quantitative PCR instrument according to claim 1, characterized in that The filter module (22) comprises a rotating disk (222) and a plurality of filter structures (223), wherein the plurality of filter structures (223) are arranged at annular intervals on the rotating disk (222), and each of the filter structures (223) is provided with the filter channel (221); the rotating disk (222) is connected to the rotation drive device (23).

3. The fluorescence quantitative PCR instrument according to claim 1, characterized in that The rotary drive device (23) comprises a first motor (231) and a transmission mechanism (232); the first motor (231) is in transmission connection with the transmission mechanism (232); and the transmission mechanism (232) is connected to the light filtering module (22).

4. The fluorescence quantitative PCR instrument according to claim 1, characterized in that The reflection module (24) and the heating module (32) are spaced apart, and the light source (21) is arranged on a side of the filter module (22) close to the heating module (32).

5. The fluorescence quantitative PCR instrument according to claim 1, characterized in that The linear drive device (33) comprises a second motor (331), a first synchronous belt (332), a linear guide rail (333) and two first synchronous wheels (334), wherein the two first synchronous wheels (334) are arranged at intervals, the second motor (331) is transmission-connected to one of the first synchronous wheels (334), the first synchronous belt (332) is transmission-connected to the two first synchronous wheels (334) at the same time, the linear guide rail (333) is arranged on the first synchronous belt (332), and the heating module (32) is arranged on the linear guide rail (333).

6. The fluorescence quantitative PCR instrument according to claim 1, characterized in that The heating module (32) includes a heating body (321) and a heating mounting frame (322), wherein the heating body (321) is provided with a plurality of positioning holes (3211), and the heating mounting frame (322) is provided with a plurality of positioning pins (3221), and the plurality of positioning holes (3211) and the plurality of positioning pins (3221) are matched one-to-one; the heating mounting frame (322) is connected to the linear drive device (33), and the heating body (321) is used to place the PCR plate (200).

7. The fluorescence quantitative PCR instrument according to claim 1, characterized in that The metal bath subsystem (30) further includes a thermal cover module (34) disposed within the instrument housing (10).

8. The fluorescence quantitative PCR instrument according to claim 7, characterized in that: The thermal cover module (34) comprises a thermal cover heating film structure (341) and a thermal cover body (342), wherein the thermal cover heating film structure (341) is detachably connected to the thermal cover body (342).

9. The fluorescent quantitative PCR instrument according to claim 8, characterized in that: The heat cover body (342) includes a first fixed structure (3421), a second fixed structure (3422) and a lifting structure (3423), wherein the first fixed structure (3421), the lifting structure (3423) and the second fixed structure (3422) are arranged in sequence, and the heat cover heating film structure (341) is detachably connected to a side of the lifting structure (3423) close to the second fixed structure (3422); The thermal cover module (34) further includes a lifting drive device (343), which is arranged on the lifting structure (3423) and is simultaneously connected to the first fixed structure (3421) and the second fixed structure (3422) to drive the lifting structure (3423) to rise and fall relative to the first fixed structure (3421) and the second fixed structure (3422).

10. The fluorescent quantitative PCR instrument according to claim 9, characterized in that: The lifting drive device (343) includes a third motor (3431), a second synchronous belt (3432), a plurality of second synchronous wheels (3433) and a plurality of screw rods (3434). The third motor (3431) is arranged on the lifting structure (3423) and is transmission-connected to the second synchronous belt (3432). The plurality of second synchronous wheels (3433) are arranged at intervals on the lifting structure (3423); the second synchronous belt (3432) is transmission-connected to the plurality of second synchronous wheels (3433), and the plurality of screw rods (3434) are transmission-connected to the plurality of second synchronous wheels (3433) respectively, and are simultaneously connected to the first fixed structure (3421) and the second fixed structure (3422).

Images