High-specificity high-flux CTC enrichment instrument

Abstract

The utility model belongs to the technical field of biological medicine equipment, and particularly relates to a high-specificity high-flux CTC (circulating tumor cell) enrichment instrument which comprises a base, a protection frame arranged on the base, a workbench, a control screen, a chip mechanism, a blood sample storage device, a blood sample recovery device, a waste liquid storage device and a liquid storage device, the liquid storage pipe is used for receiving the blood sample in the blood sample storage device and the cleaning liquid in the liquid storage device; and the conveying and control assembly is used for conveying the blood sample in the blood sample storage device and the cleaning liquid in the liquid storage device to the liquid storage pipe and the chip mechanism. Separation and enrichment of CTC in whole blood are realized without depending on means of antibody labeling and magnetic bead separation; the whole blood sample loading does not need to lyse red blood cells and separate the albuginea layer, so that simple and quick sample loading is realized; the lossless enrichment of the CTC and the white blood cells from various sources can be realized.

Description

Technical Field

[0001] The utility model belongs to the technical field of biomedical equipment, in particular to a high-specificity and high-throughput CTC enrichment instrument. Background Art

[0002] Cancer poses a major threat to human health. Due to the lack of effective and accurate early diagnosis methods, many patients are diagnosed with advanced cancer and metastasis. The metastatic spread and spread of CTCs account for more than 90% of cancer-related deaths. Cancer metastasis involves the spread of cancer cells from the initial site to form distant secondary tumors and is the main cause of death in cancer patients. According to literature reports, primary tumor cells undergo a metastatic process in the following schematic steps: (1) local invasion, whereby tumor cells detach from the primary tumor and destroy the basement membrane (which makes the tumor malignant), (2) intravasation into the blood or lymphatic circulatory system, transported through circulation and interaction with blood components, (3) arrest in the microvessels of various organs, (4) extravasation and migration into distant tissues, followed by colonization to form micrometastases, and (5) stimulation of angiogenesis, leading to growth into macrometastases (metastatic tumors). In this process, the detection of CTCs is crucial for the early diagnosis and treatment of cancer. However, CTCs exist at extremely low concentrations and are masked by billions of cells in peripheral blood, which hinders the understanding of their mechanism of action and their clinical importance.

[0003] Existing CTC detection methods mainly include immunomagnetic separation and PCR-based molecular detection. These methods can capture and detect CTCs to a certain extent, but they still have problems such as low sensitivity, low specificity, and complex operation. Microfluidic chip technology, also known as Lab-on-a-Chip technology, is a miniaturized system that integrates chemical and biological experimental operations. It uses micron-sized channels and chambers to process and analyze samples. The core advantage of this technology is that it can process fluids in a tiny volume while achieving high-efficiency and high-sensitivity analysis. Using microfluidic chips to construct a simple and portable device for biomarker detection is expected to provide a new method for future outdoor instant diagnosis and home medical care. Based on this, a high-specificity and high-throughput CTC enrichment instrument and its enrichment method are provided. Summary of the Invention

[0004] In response to the shortcomings of the above-mentioned existing technologies, a high-specificity and high-throughput CTC enrichment instrument and enrichment method are provided, which can achieve CTC sorting and enrichment in whole blood without relying on antibody labeling and magnetic bead separation. Whole blood sampling does not require red blood cell lysis or separation of the buffy coat, achieving simple and fast sampling. It can achieve non-destructive enrichment of CTCs and white blood cells from multiple sources.

[0005] In order to solve the above technical problems, the technical solution adopted by the present invention is a high-specificity and high-throughput CTC enrichment instrument, including a base, a protective frame arranged on the base, a workbench, and a control screen. It also includes a chip mechanism arranged on the base for sorting and enriching tumor cells and normal cells in the blood, a blood sample storage device for storing blood samples and providing fresh blood samples to the chip mechanism, a blood sample recovery device for recovering concentrated blood samples after loading, a waste liquid storage device for storing waste liquid, a liquid storage device for storing cleaning liquid for cleaning the blood sample storage device and the chip mechanism, a liquid storage tube for receiving the blood sample in the blood sample storage device and the cleaning liquid in the liquid storage device, and a conveying and control component for conveying the blood sample in the blood sample storage device and the cleaning liquid in the liquid storage device to the liquid storage tube and the chip mechanism.

[0006] The chip mechanism of the above-mentioned high-specificity and high-throughput CTC enrichment instrument includes a chip, a carrier plate for carrying the chip, a cover plate hinged to the carrier plate, and a lock that locks the cover plate and the carrier plate when the two are closed.

[0007] The above-mentioned high-specificity and high-throughput CTC enrichment instrument, the blood sample storage device includes a sampling rack, a slide rail provided on the sampling rack, and a slider provided on the slide rail, a supporting rack provided on the slider, a vacuum sampling tube provided on the supporting rack, a sample addition steel needle corresponding to the vacuum sampling tube provided on the top of the sampling rack, a limit plate provided on the slide rail, and a buffer block provided at the bottom of the sampling rack.

[0008] The blood sample recovery device in the aforementioned high-specificity, high-throughput CTC enrichment instrument includes two swing arms rotatably connected to the workbench. One swing arm is equipped with a double steel needle for sample extraction, and the other is equipped with a single steel needle for sample extraction. Below the two swing arms are respectively located a low-pressure concentrated blood sample recovery centrifuge tube for receiving blood samples concentrated after low-pressure loading and a high-pressure concentrated blood sample recovery centrifuge tube for receiving blood samples concentrated after high-pressure loading.

[0009] In the above-mentioned high-specificity and high-throughput CTC enrichment instrument, the liquid storage device includes an alcohol storage bottle for storing alcohol and a PBS storage bottle for storing PBS, and the waste liquid storage device is a waste liquid storage bottle for storing cleaned alcohol and PBS.

[0010] In the above-mentioned high-specificity and high-throughput CTC enrichment instrument, the structure of the liquid storage tube is a spiral structure.

[0011] The above-mentioned high-specificity, high-throughput CTC enrichment instrument, the delivery and control components include multiple delivery liquid circuits, and solenoid valves for controlling the on-off of each delivery liquid circuit, as well as a 6-position high-pressure rotary valve, a pressure sensor, a high-pressure injection pump, a pressure transmitter, and a screw slide connected to the high-pressure injection pump, and an integrated drive and control motor for driving the screw slide.

[0012] The beneficial effects of the high-specificity and high-throughput CTC enrichment instrument of the utility model are:

[0013] (1) Based on the inertial focusing principle, inertial lift and Dean drag force enable cells of different sizes to be focused at different locations in the channel. This design utilizes arcs of different radii to connect turns, combining the advantages of the classic spiral structure and the serpentine structure to achieve the effect of focusing and separating cells of different sizes over a wide flow rate range. The chip described in this design is a microfluidic structure and microfluidic chip disclosed in CN117903906A, which realizes the use of arcs of different radii to connect turns.

[0014] (2) CTC sorting and enrichment in whole blood can be achieved without relying on antibody labeling and magnetic bead separation;

[0015] (3) Whole blood sampling does not require lysis of red blood cells or separation of the buffy coat, enabling simple and quick sampling;

[0016] (4) It can achieve non-destructive enrichment of CTCs and leukocytes from various sources. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a structural diagram of the utility model;

[0018] Figure 2 Schematic diagram of the internal structure of the protection frame;

[0019] Figure 3 It is a structural schematic diagram of a blood sample recovery device;

[0020] Figure 4 is a structural schematic diagram of a blood sample storage device;

[0021] Figure 5 Schematic diagram of the chip structure;

[0022] Figure 6 This is a schematic diagram of the liquid delivery circuit of the utility model;

[0023] Figure 7 It is a structural diagram of the conveying and control components;

[0024] Figure 8 These are CTCs observed under a fluorescence microscope after being sorted by an instrument. DETAILED DESCRIPTION

[0025] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0026] like Figure 1-7As shown, a high-specificity and high-throughput CTC enrichment instrument includes a base 1, a protective frame 2 arranged on the base 1, a workbench 3, and a control screen 4. It also includes a chip mechanism arranged on the base 1 for sorting and enriching tumor cells and normal cells in the blood, a blood sample storage device for storing blood samples and providing fresh blood samples to the chip mechanism, a blood sample recovery device for recovering concentrated blood samples after loading, a waste liquid storage device for storing waste liquid, a liquid storage device for storing cleaning liquid for cleaning the blood sample storage device and the chip mechanism, a liquid storage tube 5 for receiving the blood sample in the blood sample storage device and the cleaning liquid in the liquid storage device, and a conveying and control component for conveying the blood sample in the blood sample storage device and the cleaning liquid in the liquid storage device to the liquid storage tube and the chip mechanism.

[0027] The chip mechanism includes a chip 6, a carrier plate 7 for carrying the chip 6, a cover plate 8 hinged to the carrier plate 7, and a lock 9 for locking the cover plate 8 and the carrier plate 7 when the cover plate 8 and the carrier plate 7 are closed.

[0028] The blood sample storage device includes a sampling rack 10, a slide rail 11 provided on the sampling rack 10, and a slider 12 provided on the slide rail 11. A carrier 13 is provided on the slider 12, and a vacuum sampling tube 14 is provided on the carrier 13. A sample loading steel needle 15 corresponding to the vacuum sampling tube 14 is provided on the top of the sampling rack 10, a limit plate 16 is provided on the slide rail 11, and a buffer block 17 is provided at the bottom of the sampling rack 10. This facilitates the operator to replace samples and enables accurate positioning and sampling after sample loading.

[0029] The blood sample recovery device includes two swing arms 18 rotatably connected to the workbench 3, one swing arm 18 is provided with a double steel needle 19 for sample discharge, and the other swing arm is provided with a single steel needle 20 for sample discharge. Under the two swing arms 18, there are respectively provided a low-pressure concentrated blood sample recovery centrifuge tube 21 for receiving a blood sample concentrated after low-pressure loading and a high-pressure concentrated blood sample recovery centrifuge tube 27 for receiving a blood sample concentrated after high-pressure loading.

[0030] The liquid storage device includes an alcohol storage bottle 22 for storing alcohol and a PBS storage bottle 23 for storing PBS. The waste liquid storage device is a waste liquid storage bottle for storing cleaned alcohol and PBS.

[0031] The structure of the liquid storage tube 5 is a spiral structure.

[0032] The delivery and control assembly includes multiple delivery fluid paths and solenoid valves for controlling the on / off of each delivery fluid path. It also includes a 6-position high-pressure rotary valve 24, a pressure sensor 25, a high-pressure injection pump 26, a pressure transmitter, a screw slide 28 connected to the high-pressure injection pump 26, and a drive-control integrated motor 29 for driving the screw slide 28. The rotation of the drive-control integrated motor drives the screw slide 28, thereby achieving linear reciprocating motion and precise positioning of the piston of the high-pressure injection pump 26. This realizes the change in the internal volume of the cylinder body, and cooperates with the opening and closing of the solenoid valve to achieve precise control of the liquid intake, discharge volume and flow rate.

[0033] The enrichment method of the above-mentioned high-specificity and high-throughput CTC enrichment instrument comprises the following steps:

[0034] S1, loading the diluted blood into a blood sample storage device;

[0035] S2: Select the loading program on the control screen. The blood sample in the blood sample storage device is transferred to the liquid storage tube through the delivery and control component. The blood sample in the liquid storage tube is then passed into the chip at a corresponding pressure through the liquid delivery and control component to separate tumor cells that have entered the blood. The loading program includes high-pressure loading and low-pressure loading. The low-pressure loading program is targeted at non-solid tumors such as leukemia, while the high-pressure loading program is targeted at solid tumors.

[0036] S3, collect concentrated blood samples and recover diluted blood;

[0037] S4, selecting a cleaning program on the control screen, and passing the cleaning liquid in the liquid storage device through the delivery and control component to clean the blood sample storage device and the blood sample residue in the chip mechanism;

[0038] S5, recycle the chip.

[0039] Example 1

[0040] A blood sample is drawn into a liquid reservoir 5 via a 6-position high-pressure rotary valve 24. A high-pressure syringe pump 26 then pumps the sample from the reservoir into either the high-pressure module inlet (chip inlet 1) or the low-pressure module inlet (chip inlet 9). The flow path design and corresponding pressure settings of the different inlets separate cancer cells from normal cells. This provides a cancer cell sorting and enrichment system, primarily consisting of a fluidic circuit design and fluid control system. A sample injection needle 15, an alcohol storage bottle 22, and a PBS storage bottle 23 are connected to positions 3, 5, and 6 of the 6-position high-pressure rotary valve, respectively. After aspiration, the liquid enters the liquid reservoir 5. The liquid reservoir 5 is connected to a pressure transmitter and a high-pressure syringe pump 26, which pumps the liquid out to the chip 6 at varying pressures. Positions 1, 2, and 4 of the 6-position high-pressure rotary valve 24 are connected to inlet 9 of chip 6, inlet 1 of chip 6, and a waste liquid storage bottle, respectively. Chip outlet 7 is connected to a low-pressure concentrated blood sample recovery centrifuge tube 21, and chip outlets 10 and 11 are connected to a high-pressure concentrated blood sample recovery centrifuge tube 27. Chip outlets 2, 3, 4, 5, 6, and 8 are connected to waste liquid storage bottles.

[0041] The workflow is as follows:

[0042] CTC+whole blood sample preparation:

[0043] MCF-7 cells were collected, stained with Hoechst 3342 for 15 min, washed twice with PBS, centrifuged at 1000 rpm for 3 min, counted using a hemocytometer, and a certain volume of cells (500 CTCs per ml of whole blood) was added to fresh blood from SD rats.

[0044] Instrument preparation:

[0045] Open the cover 8, load the chip, close the cover 8, and lock the lock 9;

[0046] Turn on the switch and control panel 4, enter the control program, write program instructions to clean the pipeline with alcohol, turn the high-pressure rotary valve 24 to 5; open the No. 2 solenoid valve; input 8ml of liquid into the liquid storage tube 5, with a flow rate of 4.2ml / min; delay for 100 seconds; close the No. 2 solenoid valve, turn the high-pressure rotary valve 24 to the No. 4 interface; discharge 8ml of liquid at a rate of 4.2ml / min; click to start the program;

[0047] To clean the pipeline with PBS, turn the 6-position high-pressure rotary valve 24 to 6; open the No. 1 solenoid valve; input 10 ml of liquid into the reservoir tube 5 at a flow rate of 4.2 ml / min; delay for 100 seconds; close the No. 1 solenoid valve and turn the high-pressure rotary valve to the No. 4 port (R4); discharge 10 ml of liquid at a rate of 4.2 ml / min; click to start the program;

[0048] To clean the low-pressure module with alcohol, turn the 6-position high-pressure rotary valve 24 to 5; open the No. 2 solenoid valve; input 8 ml of liquid into the liquid reservoir 5 at a flow rate of 3.5 ml / min; delay for 100 seconds; close the No. 2 solenoid valve; turn the 6-position high-pressure rotary valve 24 to the No. 1 interface; discharge 8 ml of liquid at a rate of 2.5 ml / min; click to start the program;

[0049] Clean the low-pressure module with PBS. Turn the 6-position high-pressure rotary valve 24 to 6. Open solenoid valve No. 1. Input 8 ml of liquid into reservoir tube 5 at a flow rate of 3.5 ml / min. Delay for 100 seconds. Close solenoid valve No. 1 and turn the high-pressure rotary valve to port No. 1. Discharge 10 ml of liquid at a flow rate of 2.5 ml / min. Click to start the program.

[0050] To clean the high-pressure module with alcohol, turn the 6-position high-pressure rotary valve 24 to 5; open the No. 2 solenoid valve; input 8 ml of liquid into the liquid reservoir 5 at a flow rate of 3.5 ml / min; delay for 100 seconds; close the No. 2 solenoid valve, turn the high-pressure rotary valve to the No. 2 port; discharge 8 ml of liquid at a rate of 3.5 ml / min; click to start the program;

[0051] Clean the high-pressure module with PBS. Turn the 6-position high-pressure rotary valve 24 to 6. Open solenoid valve No. 1. Input 8 ml of liquid into reservoir tube 5 at a flow rate of 3.5 ml / min. Delay for 100 seconds. Close solenoid valve No. 1. Turn the 6-position high-pressure rotary valve 24 to port No. 2. Discharge 10 ml of liquid at a flow rate of 3.5 ml / min. Click to start the program.

[0052] Clean the sample injection needle 15 with alcohol, turn the 6-position high-pressure rotary valve 24 to 5; open the No. 2 solenoid valve; input 6 ml of liquid into the liquid storage tube 5 at a flow rate of 3.5 ml / min; delay for 100 seconds; close the No. 2 solenoid valve, turn the 6-position high-pressure rotary valve 24 to the No. 3 interface; discharge 6 ml of liquid at a rate of 3.5 ml / min; click to start the program;

[0053] Wash the sample injection needle 15 with PBS, turn the 6-position high-pressure rotary valve 24 to 6; open the No. 1 solenoid valve; input 10 ml of liquid into the liquid reservoir 5 at a flow rate of 3.5 ml / min; delay for 100 seconds; close the No. 1 solenoid valve, turn the 6-position high-pressure rotary valve 24 to the No. 3 interface; discharge 10 ml of liquid at a flow rate of 3.5 ml / min; click to start the program;

[0054] Cell sorting

[0055] Perform the high-pressure loading procedure, turn the 6-position high-pressure rotary valve 24 to 3, input 7 ml of liquid into the liquid reservoir 5 at a flow rate of 2.5 ml / min; delay for 120 seconds; turn the 6-position high-pressure rotary valve 24 to port 1; discharge 7 ml of liquid at a flow rate of 3.5 ml / min; click Start Program;

[0056] Observation under a microscope

[0057] The sample after the CTC enrichment instrument was placed in a 48-well plate, centrifuged at 1000 rpm for 3 minutes, and the capture of CTCs was observed under a fluorescence microscope.

[0058] Instrument cleaning

[0059] Remove the vacuum sampling tube 14 from the carrier 13

[0060] Use PBS to clean the tubing, turn the 6-position high-pressure rotary valve 24 to 6, open the No. 1 solenoid valve, input 10 ml of liquid into the reservoir tube 5 at a flow rate of 4.2 ml / min, delay for 100 seconds, close the No. 1 solenoid valve, turn the 6-position high-pressure rotary valve 24 to the No. 4 port, discharge 10 ml of liquid at a flow rate of 4.2 ml / min, and click Start Program.

[0061] Clean the high-pressure module with PBS, turn the 6-position high-pressure rotary valve 24 to 6, open the No. 1 solenoid valve, input 8 ml of liquid into the reservoir tube 5 at a flow rate of 3.5 ml / min, delay for 100 seconds, close the No. 1 solenoid valve, turn the 6-position high-pressure rotary valve 24 to the No. 2 port, discharge 10 ml of liquid at a flow rate of 3.5 ml / min, and click Start Program.

[0062] Wash the sample injection needle 15 with PBS, turn the 6-position high-pressure rotary valve 24 to 6; open the No. 1 solenoid valve; input 10 ml of liquid into the liquid reservoir 5 at a flow rate of 3.5 ml / min; delay for 100 seconds; close the No. 1 solenoid valve, turn the 6-position high-pressure rotary valve 24 to the No. 3 interface; discharge 10 ml of liquid at a flow rate of 3.5 ml / min; click to start the program;

[0063] Rinse the tubing with PBS, turn the 6-position high-pressure rotary valve 24 to port 6, open solenoid valve No. 1, inject 10 ml of liquid into reservoir tube 5 at a flow rate of 4.2 ml / min, delay for 100 seconds, close solenoid valve No. 1, turn the 6-position high-pressure rotary valve 24 to port 4, discharge 10 ml of liquid at a flow rate of 4.2 ml / min (V 4.2), and click Start Program.

[0064] Clean the high-pressure module with PBS, turn the 6-position high-pressure rotary valve 24 to 6, open the No. 1 solenoid valve, input 8 ml of liquid into the reservoir tube 5 at a flow rate of 3.5 ml / min, delay for 100 seconds, close the No. 1 solenoid valve, turn the 6-position high-pressure rotary valve 24 to the No. 2 port, discharge 10 ml of liquid at a flow rate of 3.5 ml / min, and click Start Program.

[0065] Wash the sample injection needle 15 with PBS, turn the 6-position high-pressure rotary valve 24 to 6; open the No. 1 solenoid valve; input 10 ml of liquid into the liquid reservoir 5 at a flow rate of 3.5 ml / min; delay for 100 seconds; close the No. 1 solenoid valve, turn the 6-position high-pressure rotary valve 24 to the No. 3 interface; discharge 10 ml of liquid at a flow rate of 3.5 ml / min; click to start the program;

[0066] Use PBS to clean the pipeline, turn the 6-position high-pressure rotary valve 24 to 6; open the No. 1 solenoid valve; input 10ml of liquid into the reservoir tube 5 at a flow rate of 4.2ml / min; delay for 100 seconds; close the No. 1 solenoid valve, turn the high-pressure rotary valve to the No. 4 port; discharge 10ml of liquid at a rate of 4.2ml / min; click to start the program;

[0067] Clean the high-pressure module with PBS, turn the 6-position high-pressure rotary valve 24 to 6, open the No. 1 solenoid valve, input 8 ml of liquid into the reservoir tube 5 at a flow rate of 3.5 ml / min, delay for 100 seconds, close the No. 1 solenoid valve, turn the 6-position high-pressure rotary valve 24 to the No. 2 port, discharge 10 ml of liquid at a flow rate of 3.5 ml / min, and click Start Program.

[0068] Use PBS to clean the sampling needle, turn the 6-position high-pressure rotary valve 24 to 6; open the No. 1 solenoid valve; input 10ml of liquid into the liquid reservoir 5 at a flow rate of 3.5ml / min; delay for 100 seconds; close the No. 1 solenoid valve, turn the 6-position high-pressure rotary valve 24 to the No. 3 interface; discharge 10ml of liquid at a rate of 3.5ml / min; click to start the program;

[0069] After cleaning, the chip is recovered.

[0070] Of course, the above description is not a limitation of the present invention, and the present invention is not limited to the above examples. Changes, modifications, additions or substitutions made by ordinary technicians in this technical field within the essential scope of the present invention should also fall within the scope of protection of the present invention.

Claims

1. A high-specificity, high-throughput CTC enrichment instrument comprising a base, a protective frame mounted on the base, a workbench, and a control panel, characterized in that: It also includes a chip mechanism arranged on the base for sorting and enriching tumor cells and normal cells in the blood, a blood sample storage device for storing blood samples and providing fresh blood samples to the chip mechanism, a blood sample recovery device for recovering concentrated blood samples after sampling, a waste liquid storage device for storing waste liquid, a liquid storage device for cleaning the blood sample storage device and the chip mechanism, a liquid storage tube for receiving the blood sample in the blood sample storage device and the cleaning liquid in the liquid storage device, and a conveying and control component for conveying the blood sample in the blood sample storage device and the cleaning liquid in the liquid storage device to the liquid storage tube and the chip mechanism.

2. The high-specificity, high-throughput CTC enrichment instrument according to claim 1, characterized in that: The chip mechanism comprises a chip, a carrier plate for carrying the chip, a cover plate hinged to the carrier plate, and a lock for locking the cover plate and the carrier plate when the cover plate and the carrier plate are closed.

3. The high-specificity, high-throughput CTC enrichment instrument according to claim 2, wherein: The blood sample storage device includes a sampling rack, a slide rail arranged on the sampling rack, and a slider arranged on the slide rail. A carrier is provided on the slider, a vacuum sampling tube is provided on the carrier, a sample adding steel needle corresponding to the vacuum sampling tube is provided on the top of the sampling rack, a limit plate is provided on the slide rail, and a buffer block is provided at the bottom of the sampling rack.

4. The high-specificity, high-throughput CTC enrichment instrument according to claim 3, wherein: The blood sample recovery device includes two swing arms rotatably connected to the workbench, one swing arm is provided with a double steel needle for sample discharge, and the other swing arm is provided with a single steel needle for sample discharge. Under the two swing arms are respectively provided a low-pressure concentrated blood sample recovery centrifuge tube for receiving a blood sample concentrated after low-pressure sampling and a high-pressure concentrated blood sample recovery centrifuge tube for receiving a blood sample concentrated after high-pressure sampling.

5. The high-specificity, high-throughput CTC enrichment instrument according to claim 4, characterized in that: The liquid storage device includes an alcohol storage bottle for storing alcohol and a PBS storage bottle for storing PBS. The waste liquid storage device is a waste liquid storage bottle for storing cleaned alcohol and PBS.

6. The high-specificity and high-throughput CTC enrichment instrument according to claim 5, characterized in that: The structure of the liquid storage tube is a spiral structure.

7. The high-specificity, high-throughput CTC enrichment instrument according to claim 6, characterized in that: The delivery and control component includes multiple delivery fluid circuits and solenoid valves for controlling the on and off of each delivery fluid circuit. It also includes a 6-position high-pressure rotary valve, a pressure sensor, a high-pressure injection pump, a pressure transmitter, a screw slide connected to the high-pressure injection pump, and an integrated drive and control motor for driving the screw slide.

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