Anti-toppling and anti-overflowing PCR (Polymerase Chain Reaction) reagent tube

Abstract

The utility model belongs to the field of biomedical engineering, and particularly provides an anti-toppling and anti-overflow PCR (Polymerase Chain Reaction) reagent tube which comprises a supporting structure, a PCR tube, a conical anti-overflow piece and a cover, and the supporting structure is arranged on the periphery of the PCR tube and is used for supporting the PCR tube to be vertical; the conical anti-overflow piece is arranged in the PCR tube, the upper end of the conical anti-overflow piece is in sealed connection with the upper part of the PCR tube, the conical anti-overflow piece is frustum-shaped, the lower end of the conical anti-overflow piece is a small-diameter end, an exhaust hole is formed in the side wall of the conical anti-overflow piece, and a pipette operation hole is formed in the lower end of the conical anti-overflow piece; the cover comprises a sealing position for sealing the PCR tube and a conical cover arranged at the lower end of the sealing position; the lower end of the conical cover is arranged on the radial inner side of the conical anti-overflow piece and is used for reducing the space at the upper part of the PCR tube. According to the utility model, the PCR reagent tube does not depend on a special storage rack any more, so that the PCR tube can be directly placed on an experiment table; and even if the PCR tube topples over, the liquid reagent does not overflow out of the tube to cause biological pollution.

Description

Technical Field

[0001] The utility model belongs to the field of biomedical engineering, and in particular relates to a PCR reagent tube which is anti-dumping and anti-overflow. Background Art

[0002] Biological PCR laboratories are based on PCR technology, which stands for polymerase chain reaction. PCR is a molecular biology technique used to amplify specific DNA fragments in vitro. The basic principle of PCR technology is similar to the natural replication process of DNA. Its specificity relies on oligonucleotide primers that are complementary to the target sequence at both ends. PCR consists of three basic reaction steps: denaturation-annealing-extension: ① Denaturation of template DNA: After the template DNA is heated to 90-96℃ for a certain period of time, the double-stranded template DNA or the double-stranded DNA formed by PCR amplification is dissociated, making it a single strand so that it can bind to the primer and prepare for the next round of reaction; ② Annealing (renaturation) of template DNA and primer: After the template DNA is denatured into a single strand by heating, the temperature is reduced to 55-65℃, and the primers pair and bind to the complementary sequence of the single strand of template DNA; ③ Extension of primer: The DNA template-primer complex is heated to 70-75℃ and under the action of DNA polymerase (such as TaqDNA polymerase), using dNTP as the reaction raw material and the target sequence as the template, a new semi-conservative copy chain complementary to the template DNA chain is synthesized according to the principle of base complementary pairing and semi-conservative replication. By repeating the three processes of denaturation-annealing-extension, more "semi-conservative copy chains" can be obtained, and this new chain can become the template for the next cycle. It takes 2 to 4 minutes to complete one cycle, and the target gene to be amplified can be amplified millions of times in 2 to 3 hours.

[0003] There are five main substances involved in PCR, namely: primers (PCR primers are DNA fragments, and the primers for DNA replication in cells are RNA chains), enzymes, dNTPs, templates, and buffers (Mg is required). 2+ ). Completing a PCR experiment requires adding multiple reagents containing at least these five substances into a PCR tube.

[0004] Conventional PCR tubes currently feature a tapered bottom. This tapered structure ensures that small amounts of liquid are more evenly contacted with the thermal control module of the PCR instrument, effectively transferring temperature. Precise temperature control is crucial for the denaturation, annealing, and extension processes of PCR. Due to the tapered bottom of PCR tubes, a dedicated rack is required to stably position the tubes upright on a tabletop when adding reagents. Chinese invention patent application CN201710411444.8 provides a PCR tube, the structure of which is a concave plane mirror provided on the top of the tube cap, the top surface of the concave plane mirror is lower than the top surface of the tube cap, and an outwardly protruding arc step is provided on the circumferential outer surface of the bottom end of the tube cap. The longitudinal section of the arc step is arched, and a groove is provided below the inner tube opening of the tube body to match the arc step. The arc step and the groove have an interference fit, and the longitudinal section of the tube opening is an inverted isosceles trapezoid. The upper half of the tube body is a cylindrical tube body, and the lower half is a conical tube body. The conical tube body is surrounded by a conical tube wall. The bottom of the conical tube body is the tube bottom, and the lower end surface of the tube bottom is the tube bottom surface, which is a flat surface. The bottom of the tube body of this invention is designed to be flat, which is convenient for upright placement on a desktop. However, in actual operation, this PCR tube is still easy to be bumped.

[0005] In addition, during the PCR experiment, it is often easy for the experimenter to overturn the PCR reagent tube due to misoperation, causing the reagent to overflow onto the table and cause biological contamination.

[0006] Therefore, there is a need in the art for a PCR reagent tube that is anti-dumping and anti-overflow. Utility Model Content

[0007] Therefore, the utility model provides a PCR reagent tube that is anti-dumping and anti-overflow, the PCR reagent tube comprising a support structure (1), a PCR tube (2), a conical anti-overflow member (3) and a cover (4), the PCR tube (2) being tapered at the bottom and being used to accommodate substances participating in a polymerase chain reaction, the support structure (1) being arranged on the periphery of the PCR tube (2) and being used to support the PCR tube in an upright position, the support structure (1) comprising a hollow cylindrical support body (101) and a through-hole-shaped light-collecting hole (102) provided on the side wall of the support body (101), the support structure (1) being fixedly connected to the outer wall of the PCR tube (2) at its connection point (103); the conical anti-overflow member (3) and the cover (4) are provided on the side wall of the support body (101). The member (3) is arranged inside the PCR tube (2), and the upper end of the conical overflow prevention member (3) is sealed and connected to the upper part of the PCR tube (2). The conical overflow prevention member (3) is in a cone shape and its lower end is a small-diameter end. An exhaust hole (301) is opened on the side wall of the conical overflow prevention member (3), and a pipette operation hole (302) is provided at the lower end of the conical overflow prevention member (3); the cover (4) is used to match the PCR tube (2), and the cover (4) includes a sealing position (401) for sealing the PCR tube (2) and a conical cover (402) arranged at its lower end. The lower end of the conical cover (402) is arranged on the radial inner side of the conical overflow prevention member (3) and is used to reduce the space on the upper part of the PCR tube (2).

[0008] In a specific embodiment, the support structure (1) and the PCR tube (2) are integrated and / or the PCR tube (2) and the conical anti-overflow component (3) are integrated.

[0009] In a specific embodiment, the cover (4) is connected to the outer wall of the support structure (1) or the PCR tube (2), and the cover (4) is integrated with the support structure (1), the PCR tube (2) and the conical anti-overflow part (3).

[0010] In a specific embodiment, the outer wall of the support structure (1) is cylindrical.

[0011] In a specific embodiment, two or more exhaust holes (301) are opened on the side wall of the conical overflow prevention member (3), and the exhaust holes (301) are symmetrically arranged in the circumferential direction.

[0012] In a specific embodiment, the distance between the exhaust hole (301) and the upper edge and the lower edge of the conical overflow prevention member (3) is greater than h / 10, where h is the height of the conical overflow prevention member (3).

[0013] In a specific embodiment, a cylindrical section is provided above the bottom conical section of the PCR tube (2), and the top of the support structure (1) is fixedly connected to the outer wall of the cylindrical section of the PCR tube (2).

[0014] The utility model has at least the following beneficial effects:

[0015] The PCR reagent tubes described in this utility model no longer require dedicated racks, allowing them to be placed directly on a laboratory table. The conical overflow prevention member provided in this utility model prevents the liquid reagent in the PCR tube from overflowing and causing biological contamination even if the PCR tube is tipped over. The lid of this utility model includes a conical cap disposed at its lower end. The lower end of the conical cap is disposed radially inward of the conical overflow prevention member and is used to reduce the space above the PCR tube, thereby preventing evaporation of the reagent during PCR temperature cycling, thereby ensuring accurate reaction results. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is the overall structural diagram of the PCR reagent tube described in the present invention.

[0017] Figure 2 This is a structural diagram of the PCR reagent tube of the present invention without the cover.

[0018] Figure 3 This is a structural diagram of the lid of the PCR reagent tube described in the present invention.

[0019] In the figure: 1. Support structure, 2. PCR tube, 3. Conical anti-overflow part, 4. Lid, 101. Support body, 102. Lighting hole, 103. Connection, 301. Exhaust hole, 302. Pipette operation hole, 401. Sealing position, 402. Conical cover. DETAILED DESCRIPTION

[0020] like Figures 1 to 3As shown, the utility model provides a PCR reagent tube that is anti-dumping and anti-overflow, the PCR reagent tube comprising a support structure (1), a PCR tube (2), a conical anti-overflow member (3) and a cover (4), the PCR tube (2) being tapered at the bottom and being used to accommodate substances participating in a polymerase chain reaction, the support structure (1) being arranged on the periphery of the PCR tube (2) and being used to support the PCR tube in an upright position, the support structure (1) comprising a hollow cylindrical support body (101) and a through-hole-shaped light-collecting hole (102) provided on the side wall of the support body (101), the support structure (1) being fixedly connected to the outer wall of the PCR tube (2) at its connection (103); the conical anti-overflow member (3) and the cover (4) are provided on the side wall of the support body (101). The member (3) is arranged inside the PCR tube (2), and the upper end of the conical overflow prevention member (3) is sealed and connected to the upper part of the PCR tube (2). The conical overflow prevention member (3) is in a cone shape and its lower end is a small diameter end. An exhaust hole (301) is opened on the side wall of the conical overflow prevention member (3), and a pipette operation hole (302) is provided at the lower end of the conical overflow prevention member (3); the cover (4) is used to match the PCR tube (2), and the cover (4) includes a sealing position (401) for sealing the PCR tube (2) and a conical cover (402) arranged at its lower end. The lower end of the conical cover (402) is arranged on the radial inner side of the conical overflow prevention member (3) and is used to reduce the space on the upper part of the PCR tube (2). The support structure (1), the PCR tube (2) and the conical overflow prevention member (3) are integrated. The lid (4) is connected to the outer wall of the PCR tube (2), and the lid (4) is integrated with the support structure (1), the PCR tube (2) and the conical overflow prevention member (3). The outer wall of the support structure (1) is cylindrical. Two symmetrically arranged exhaust holes (301) are provided on the side wall of the conical overflow prevention member (3). The distance between the exhaust holes (301) and the upper and lower edges of the conical overflow prevention member (3) is greater than h / 10, where h is the height of the conical overflow prevention member (3). A cylindrical section is provided above the bottom conical section of the PCR tube (2), and the top of the support structure (1) is fixedly connected to the outer wall of the cylindrical section of the PCR tube (2).

[0021] The utility model is provided with a support structure (1), specifically a hollow cylindrical support column, so that the PCR tube (2) no longer needs to rely on a dedicated rack, and the PCR tube can be directly placed on the experimental table. A light hole (102) is provided on the support structure, which is a reserved PCR light hole, that is, a fluorescence collection hole.

[0022] The PCR tube (2) of the utility model is used for storing reagents such as DNA templates, primer reagents, enzyme reagents and buffer solutions, and the conical structure at the bottom is its temperature-controlled lighting area, belonging to the core reaction area of ​​PCR.

[0023] The lower end of the conical overflow prevention part (3) provided in the present invention is provided with a pipette operation hole (302), the size of which is conducive to the operation of the gun head of a general pipette, so that the PCR reagent can be conveniently added to the bottom of the PCR tube. An exhaust hole (301) is provided on the side wall of the conical overflow prevention part (3). When adding reagents to the PCR tube (2) through the pipette operation hole (302), the exhaust hole (301) is used to balance the pressure inside and outside the PCR tube (2), so that the reagents can be smoothly added to the PCR tube (2). In particular, two exhaust holes (301) are relatively provided on the side wall of the conical overflow prevention part (3), and the ventilation effect is the best. The upper end of the conical overflow prevention part (3) provided in the present invention inside the PCR tube (2) is sealed and connected to the upper part of the PCR tube (2). Therefore, even if the PCR tube falls over, the liquid reagent in the PCR tube will not overflow to the outside of the tube and overflow to the table to cause biological contamination.

[0024] The lid (4) of the utility model includes a sealing position (401) for sealing the PCR tube (2). After all reagents are added to the PCR tube (2), the lid is closed, and the lid (4) is tightly connected to the PCR tube (2) at the sealing position (401) to complete the sealing. During the entire polymerase chain reaction process, the PCR tube (2) will not be contaminated by the outside.

[0025] The lid (4) of the utility model includes a conical cover (402) arranged at its lower end. The lower end of the conical cover (402) is arranged on the radial inner side of the conical anti-overflow component (3) and is used to reduce the space above the PCR tube (2), thereby preventing the evaporation of the reagent during the PCR temperature cycle change process, thereby preventing the change of the reagent concentration after condensation, and ensuring the accuracy of the reaction results.

[0026] Preferably, the support structure (1), PCR tube (2) and conical anti-overflow component (3) are integrated to facilitate production and manufacturing, and the integrated arrangement of the PCR tube (2) and the conical anti-overflow component (3) can also achieve a better sealing and anti-overflow effect of the PCR reagent tube.

[0027] It will be easily understood by those skilled in the art that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A PCR reagent tube that prevents tipping and overflowing, characterized in that: The PCR reagent tube comprises a support structure (1), a PCR tube (2), a conical anti-overflow part (3) and a cover (4); the PCR tube (2) is conical at the bottom and is used to accommodate substances participating in the polymerase chain reaction; the support structure (1) is arranged on the periphery of the PCR tube (2) and is used to support the PCR tube to stand upright; the support structure (1) comprises a hollow cylindrical support body (101) and a through-hole-shaped light-collecting hole (102) provided on the side wall of the support body (101); the support structure (1) is fixedly connected to the outer wall of the PCR tube (2) at its connection point (103); the conical anti-overflow part (3) is arranged on the PCR tube (2) ) inside, and the upper end of the conical overflow prevention member (3) is sealed and connected to the upper part of the PCR tube (2), the conical overflow prevention member (3) is in a cone shape and its lower end is a small diameter end, an exhaust hole (301) is opened on the side wall of the conical overflow prevention member (3), and a pipette operation hole (302) is provided at the lower end of the conical overflow prevention member (3); the lid (4) is used to match the PCR tube (2), the lid (4) includes a sealing position (401) for sealing the PCR tube (2) and a conical cover (402) arranged at its lower end, the lower end of the conical cover (402) is arranged on the radial inner side of the conical overflow prevention member (3) and is used to reduce the space on the upper part of the PCR tube (2).

2. The PCR reagent tube according to claim 1, characterized in that: The support structure (1) and the PCR tube (2) are integrated and / or the PCR tube (2) and the conical anti-overflow component (3) are integrated.

3. The PCR reagent tube according to claim 1, characterized in that: The cover (4) is connected to the outer wall of the support structure (1) or the PCR tube (2), and the cover (4) is integrated with the support structure (1), the PCR tube (2) and the conical anti-overflow part (3).

4. The PCR reagent tube according to claim 1, characterized in that The outer wall of the support structure (1) is cylindrical.

5. The PCR reagent tube according to claim 1, characterized in that: Two or more exhaust holes (301) are opened on the side wall of the conical overflow prevention member (3), and the exhaust holes (301) are symmetrically arranged in the circumferential direction.

6. The PCR reagent tube according to claim 1, characterized in that: The distances between the exhaust hole (301) and the upper edge and the lower edge of the conical overflow prevention member (3) are both greater than h / 10, where h is the height of the conical overflow prevention member (3).

7. The PCR reagent tube according to claim 1, characterized in that: A cylindrical section is provided above the bottom conical section of the PCR tube (2), and the top of the support structure (1) is fixedly connected to the outer wall of the cylindrical section of the PCR tube (2).

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