Disposable adjustable device for cell scratch experiment

Abstract

The utility model provides a disposable adjustable device for a cell scratch experiment. The cell culture plate scratching device comprises scratching arms, fixing cross rods and vertical rods, the specification of the scratching arms is matched with the diameter of the bottom face of a culture hole of a cell culture plate to be placed, a plurality of sawtooth structures distributed at preset intervals are arranged on the upper surfaces of the scratching arms, each folding arm is connected with the corresponding vertical rod, the vertical rods are connected through the fixing cross rods, and the fixing cross rods are connected with the scratching arms. A lifting partition convenient for a worker to lift is arranged above the fixed cross rod, a dismounting crease is arranged at the bottom of the fixed cross rod, and the length of the fixed cross rod is subjected to preset breaking adjustment based on the specifications of different to-be-placed cell culture plates. According to the utility model, the length of the scratching arm and the height of the fixed cross rod can be adjusted according to the depth and the diameter of the culture hole, so that the scratching arm is attached to the bottom of the culture hole, then cells are inoculated on two sides of the scratching arm, and the phenomena of scratching deviation and the like caused by improper operation are avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of cell scratch experiments, in particular to a disposable adjustable device used for cell scratch experiments. Background Art

[0002] Cancer has long been a significant threat to human health. Numerous studies have been conducted on this topic, and improving research methods will accelerate progress in conquering this disease. Migration, a fundamental cellular function, is a hallmark of tumor cell metastasis. Tumor cells demonstrating strong migration abilities in both in vitro and in vivo experiments indicate that solid tumors possess a robust capacity for distant metastasis. Therefore, studying tumor cell migration is valuable for assessing the degree of malignancy.

[0003] However, due to the influence of the experimenter's experience and operating techniques, problems that affect the experimental results are easy to occur. The cell scratch test is used to evaluate whether the migration ability of cells is affected by external stimulation. Since the experimental operation is simple and the experimental conditions are relatively loose, it can quickly detect the degree of influence on the basic functions of tumor cells. It is a relatively commonly used research method in the field of tumor research. At present, the common process in the operation process is to inoculate cells of appropriate density in a six-well plate. When the bottom of the six-well plate is almost covered, use the pipette tip to make a vertical line along the central axis of the six-well plate. This operation requires the experimenter to have a certain amount of experience, and the drawn line is easily offset due to improper operation. Secondly, the process of using the gun tip to make the line is easy to damage the cells in the drawn area. At the same time, the edge of the drawn area appears irregular when observed under a microscope, which affects the results. Since the results of this experiment need to be observed under a microscope, if the experimenter does not make a significant mark that does not affect the observation, it is easy to lose the specified field of view required for detection. Due to the influence of experimental consumables, different batches of gun tips will directly affect the consistency and repeatability of the experimental results. Therefore, there is an urgent need to provide a disposable adjustable device for cell scratch experiments to achieve a simple and convenient cell scratch experiment. Utility Model Content

[0004] In response to the above technical problems, a disposable adjustable device for cell scratch experiment is provided. The technical means adopted by the utility model are as follows:

[0005] A disposable adjustable device for cell scratch experiments includes a scratch arm, a fixed cross bar and a vertical bar. The specifications of the scratch arm match the bottom diameter of the culture well of the cell culture plate to be placed. The upper surface of the scratch arm has a plurality of serrated structures arranged at preset intervals. Each folding arm is connected to its corresponding vertical bar, and the vertical bars are connected by a fixed cross bar. A pulling barrier is provided above the fixed cross bar to facilitate lifting by staff. A disassembly fold is provided at the bottom of the fixed cross bar. Based on the specifications of different cell culture plates to be placed, the length of the fixed cross bar is adjusted by preset breaking.

[0006] Furthermore, the cell culture plate to be placed is a six-well cell culture plate, and the six-well cell culture plate has three groups of culture wells symmetrically arranged in pairs along its length direction. The vertical rods include two groups of left support vertical rods, a middle support vertical rod and a right support vertical rod, and each support vertical rod is connected to the scratch arm.

[0007] Furthermore, left vertical rod latches and right vertical rod latches are respectively provided on opposite surfaces of the left and right sides of the same row, and the bottom of the fixed cross bar is provided with left side latches of the fixed cross bar and right side latches of the fixed cross bar that cooperate with the left vertical rod latches and the right vertical rod latches.

[0008] Furthermore, the disassembly fold includes a left disassembly fold and a right disassembly fold, the left disassembly fold is arranged in the middle section between the left support vertical rod and the middle support vertical rod, and the right disassembly fold is arranged in the middle section between the middle support vertical rod and the right support vertical rod.

[0009] Furthermore, the scratching arm has a length of 33-37 mm, a width of 0.1-1 mm, and a thickness of 3-5 mm.

[0010] Furthermore, the support vertical rod has a height of 15-25 mm, a width of 2-3 mm, and a thickness of 1-5 mm.

[0011] Furthermore, the fixed cross bar has a length of 110-130 mm, a width of 3-10 mm, and a thickness of 1-5 mm.

[0012] Furthermore, the disassembly fold angle is 30°-45°, its width is 0.1-0.3mm, and its depth is 0.1-0.5mm.

[0013] Furthermore, the left vertical rod latching teeth and the left side latching teeth of the fixed cross bar, the right vertical rod latching teeth and the right side latching teeth of the fixed cross bar engage with each other, the bevel angle is 45°, and the movable range of the vertical rod latching teeth is 5-10mm.

[0014] The utility model has a simple structure. After adding culture medium, the user can directly put the scratch device into the six-well plate, and adjust the length of the scratch arm and the height of the fixed cross bar according to the depth and diameter of the culture well so that the scratch arm fits the bottom of the culture well. Then, the cells are inoculated on both sides of the scratch arm, avoiding the phenomenon of scratch deviation caused by improper operation; and the scratch area is kept consistent by the width of the scratch arm, ensuring the repeatability and consistency of the experiment; when the inoculated cells cover both sides of the scratch arm, the device can be directly lifted vertically to form a unified scratch area, reducing the damage to the cells caused by the experimental operation and avoiding the irregular shape of the edge of the scratch area; by counting the simple folds on the scratch arm or directly breaking the simple fold corresponding to the observation area, the scratch arm is placed in during observation, and the corresponding area is the target observation area, which will effectively eliminate the problem of loss of field of view during operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0016] Figure 1 This is a schematic diagram of the overall top view of the structure of the utility model;

[0017] Figure 2 This is a front view of the structure of the present invention without the lifting barrier installed;

[0018] Figure 3 This is a schematic diagram of the overall left-side structure of the present invention;

[0019] In the figure: 1. Scratching arm; 2. Fixed crossbar; 3. Left disassembly crease; 4. Right disassembly crease; 5. Left supporting vertical bar; 6. Middle supporting vertical bar; 7. Right supporting vertical bar; 8. Left vertical bar latch; 9. Right vertical bar latch; 10. Left side latch of fixed crossbar; 11. Right side latch of fixed crossbar; 12. Lifting barrier. DETAILED DESCRIPTION

[0020] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the embodiments described are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0021] like Figures 1 to 3 As shown, an embodiment of the present invention discloses a disposable adjustable device for cell scratch experiments, comprising a scratch arm 1, a fixed cross bar 2 and a vertical bar. The specifications of the scratch arm match the bottom diameter of the culture well of the cell culture plate to be placed. The upper surface of the scratch arm has a plurality of serrated structures arranged at preset intervals. Each folding arm is respectively connected to its corresponding vertical bar, and the vertical bars are connected by a fixed cross bar. A lifting barrier 12 is provided above the fixed cross bar for convenience of lifting by the staff. A disassembly fold is provided at the bottom of the fixed cross bar. Based on the specifications of different cell culture plates to be placed, the length of the fixed cross bar is adjusted by preset breaking.

[0022] The cell culture plate to be placed is a six-well cell culture plate, which has three groups of culture wells symmetrically arranged in pairs along its length direction. The vertical rods include two groups of left support vertical rods, a middle support vertical rod and a right support vertical rod, and each support vertical rod is connected to the scratch arm.

[0023] On the opposite surfaces of the vertical bars on the left and right sides of the same row, there are respectively provided with left vertical bar latches 8 and right vertical bar latches 9 arranged in a longitudinal array. The bottom of the fixed cross bar is provided with a fixed cross bar left latch 10 and a fixed cross bar right latch 11 that cooperate with the left vertical bar latches and the right vertical bar latches.

[0024] The disassembly fold includes a left disassembly fold 3 and a right disassembly fold 4. The left disassembly fold is set in the middle section between the left support vertical rod 5 and the middle support vertical rod 6, and the right disassembly fold is set in the middle section between the middle support vertical rod 6 and the right support vertical rod 7.

[0025] The scratch arm has a length of 33-37 mm, a width of 0.1-1 mm, and a thickness of 3-5 mm.

[0026] The support vertical rod has a height of 15-25 mm, a width of 2-3 mm, and a thickness of 1-5 mm.

[0027] The fixed cross bar has a length of 110-130 mm, a width of 3-10 mm, and a thickness of 1-5 mm.

[0028] The disassembly fold angle is 30°-45°, the width is 0.1-0.3mm, and the depth is 0.1-0.5mm.

[0029] The left vertical rod latching teeth and the left side latching teeth of the fixed cross bar, the right vertical rod latching teeth and the right side latching teeth of the fixed cross bar are engaged with each other, the bevel angle is 45 degrees, and the movable range of the vertical rod latching teeth is 5-10mm.

[0030] Each component of the present invention can be made of polystyrene. Specifically, it is a disposable cell scratch experiment device. It can be adjusted according to the depth and diameter differences of the six-well culture plate culture wells of different manufacturers. Specifically, the adjustment can be achieved by breaking the serrations of the scratch arm 1; the height of the device is adjusted by adjusting the degree of downward pressure of the fixed crossbar. According to different experimental requirements, there may be 2, 4, or 6 culture wells for scratching. In this case, the corresponding number of requirements can be met by breaking the crease setting. During the cell scratch experiment, the experimenter can adjust the device according to their own experimental needs. The other end can also be retained for later use, avoiding waste of the entire section of material.

[0031] When performing a cell scratch experiment, the device is first placed in a culture well, followed by a certain amount of culture medium. Finally, cells of the desired concentration are added to both sides of the scratch arm 1. The cells are then cultured until they cover the bottom of the well or reach 80-90% of the well bottom. The device is then lifted vertically upward using the lifting blocks 12 at the upper ends of the left and right support rods. The culture continues until the experimental requirements are met, and the results can be observed. The area of ​​the scratch arm 1 determines the initial scratch area, ensuring repeatability and consistency of the experiment.

[0032] This device can adapt to six-well plates with different parameters. Experimenters can adjust the device according to their needs, making the application of the device more flexible and not limited to a certain model of six-well plates. It has a simple structure and is easier to commercialize. All accessories can be sterilized uniformly, and users can use it without further operation.

[0033] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A disposable adjustable device for cell scratch test, characterized in that: It includes a scratching arm, a fixed cross bar and a vertical bar. The specifications of the scratching arm match the bottom diameter of the culture well where the cell culture plate is to be placed. The upper surface of the scratching arm has a plurality of serrated structures arranged at preset intervals. Each folding arm is connected to its corresponding vertical bar, and the vertical bars are connected by a fixed cross bar. A pulling barrier is provided above the fixed cross bar for the convenience of staff to pull. A disassembly fold is provided at the bottom of the fixed cross bar. Based on the specifications of different cell culture plates to be placed, the length of the fixed cross bar is adjusted by preset breaking.

2. The disposable adjustable device for cell scratch test according to claim 1, characterized in that: The cell culture plate to be placed is a six-well cell culture plate, which has three groups of culture wells symmetrically arranged in pairs along its length direction. The vertical rods include two groups of left support vertical rods, a middle support vertical rod and a right support vertical rod, and each support vertical rod is connected to the scratch arm.

3. The disposable adjustable device for cell scratch test according to claim 1, characterized in that: Left vertical rod latches and right vertical rod latches are arranged in a longitudinal array on opposite surfaces of the left and right sides of the same row, and the bottom of the fixed cross bar is provided with fixed cross bar left latches and fixed cross bar right latches that cooperate with the left vertical rod latches and right vertical rod latches.

4. The disposable adjustable device for cell scratch test according to claim 1, characterized in that: The disassembly fold includes a left disassembly fold and a right disassembly fold. The left disassembly fold is arranged in the middle section between the left support vertical rod and the middle support vertical rod, and the right disassembly fold is arranged in the middle section between the middle support vertical rod and the right support vertical rod.

5. The disposable adjustable device for cell scratch test according to claim 1, characterized in that: The scratch arm has a length of 33-37 mm, a width of 0.1-1 mm, and a thickness of 3-5 mm.

6. The disposable adjustable device for cell scratch test according to claim 1, characterized in that: The vertical rod has a height of 15-25 mm, a width of 2-3 mm, and a thickness of 1-5 mm.

7. The disposable adjustable device for cell scratch test according to claim 1, characterized in that: The fixed cross bar has a length of 110-130 mm, a width of 3-10 mm, and a thickness of 1-5 mm.

8. The disposable adjustable device for cell scratch test according to claim 1, characterized in that: The disassembly fold angle is 30°-45°, the width is 0.1-0.3mm, and the depth is 0.1-0.5mm.

9. The disposable adjustable device for cell scratch test according to claim 3, characterized in that: The left vertical rod latching teeth and the left side latching teeth of the fixed cross bar, the right vertical rod latching teeth and the right side latching teeth of the fixed cross bar are engaged with each other, the bevel angle is 45 degrees, and the movable range of the vertical rod latching teeth is 5-10mm.

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