Gel type culture medium pipetting device

Abstract

The utility model discloses a gelling type culture medium pipetting device which comprises an upper adjusting cap, a lower adjusting cap, a lower adjusting cap and a lower adjusting cap, wherein the upper adjusting cap is provided with an upper cavity with a downward opening; the lower adjusting cap is provided with a lower cavity with an upward opening, and the lower adjusting cap is coaxially screwed in the upper adjusting cap, so that the upper cavity and the lower cavity are communicated to form an adjusting cavity; the pressing cap is arranged on the top wall of the upper adjusting cap in a penetrating mode, and the lower edge of the pressing cap is horizontally turned outwards to form a limiting clamping plate sliding up and down along the upper cavity; the taper pipe is a double-layer pipe which is vertically fixed on the bottom wall of the lower adjusting cap in a penetrating manner and is provided with an interlayer in the middle, and a heating resistance wire is arranged in the interlayer of the taper pipe; the suction tube is an elastic bag body arranged in the adjusting cavity, the elastic bag body is provided with an opening communicated with the taper tube in a sealed mode, and the pressing cap applies force to the elastic bag body. The utility model has the advantages that the extrusion height of the elastic bag body is accurate and controllable, so that the accurate liquid suction and injection quantity of the taper pipe is ensured; liquid sucked in the taper pipe can be continuously heated, and the liquid sucked in the taper pipe is effectively prevented from being cooled and solidified.

Description

Technical Field

[0001] The utility model relates to the technical field of microbial detection, in particular to a gel-type culture medium pipetting device. Background Art

[0002] Gelation refers to the phenomenon that some hydrophilic polymer solutions, such as aqueous gelatin and agar solutions, are viscous, mobile liquids under warm conditions. When the temperature drops, the polymer solution forms a network structure, with the water dispersion medium contained within it, forming a non-flowing semi-solid. Gelling media can eliminate the fluidity of a solution, transforming it into a colloidal medium with elasticity and support. They are widely used in microbial testing experiments, such as agar-containing MH media for testing microbial resistance to antibiotics. After adding agar, MH media is solid at room temperature and liquid at around 40°C.

[0003] Gelled culture media often need to be in different states during different test processes. For example, when packaging before culturing microorganisms in gelled culture media, it needs to be packaged in a liquid state, and when culturing microorganisms, it needs to be separated and purified on a solid culture medium. For example, when inoculating bacteria in certain drug sensitivity tests, the culture medium needs to be in a liquid state. After transferring the liquid culture medium to the test plate card, it needs to be cultured in a solid state.

[0004] At this time, a dropper is a common device for transferring liquids. It is composed of a rubber cap and a pipette. By pressing the rubber cap, the liquid is sucked into the pipette, and then the liquid in the pipette can be discharged by pressing the rubber cap again, thereby achieving rapid suction and transfer of liquid. Existing droppers cannot accurately control the amount of liquid when sucking and discharging liquid. Although some droppers have scales, it is necessary to observe the liquid level and align the scales with the naked eye when using them, and it is necessary to maintain the accuracy and stability of the pressure of the rubber cap to achieve quantitative suction of the liquid. The operation process is difficult to control, and it is often necessary to press the rubber cap multiple times and constantly adjust the pressure, which wastes time and has low control accuracy. In addition, due to the low-temperature coagulation of gelling culture media, the temperature of the liquid in the existing dropper will drop rapidly after aspiration. Therefore, when using a dropper to aspirate such culture media, solidification will occur, resulting in the inability to pipette, affecting the experimental process. Summary of the Invention

[0005] The utility model aims to provide a gelling culture medium liquid transfer device, which can quantitatively aspirate the gelling culture medium and keep the gelling culture medium in a liquid state.

[0006] In order to achieve the above purpose, the present invention can adopt the following technical solutions:

[0007] The gelling culture medium pipetting device of the utility model comprises:

[0008] An upper adjusting cap having an upper chamber with a downward opening;

[0009] A lower adjusting cap having a lower chamber with an upward opening, wherein the lower adjusting cap is coaxially screwed into the upper adjusting cap so that the upper chamber and the lower chamber are connected to form an adjusting chamber;

[0010] A pressing cap is inserted into the top wall of the upper adjusting cap, and the lower edge of the pressing cap is horizontally turned outward to form a limiting card that slides up and down along the upper chamber;

[0011] The conical tube is a double-layer tube vertically fixed on the bottom wall of the lower regulating cap and having an interlayer cavity in the middle. A heating resistance wire is provided in the interlayer cavity of the conical tube;

[0012] The suction tube is an elastic capsule arranged in the regulating cavity. The elastic capsule is provided with an opening which is sealed and communicated with the cone tube. The pressing cap applies force to the elastic capsule.

[0013] Furthermore, the elastic bladder is a rubber cap or a bellows expansion tube, which can provide liquid discharge force or liquid absorption force by utilizing the contraction and expansion of the elastic bladder itself.

[0014] Furthermore, the center of the upper end surface of the elastic sac is concave to form a positioning groove, and the pressing cap is provided with a positioning protrusion that is adapted to engage with the positioning groove. The pressing cap, the conical tube and the elastic sac are coaxially arranged, so that the upper end of the elastic sac can be firmly attached to the pressing cap, and ensure that the elastic sac always remains coaxial with the pressing cap and the conical tube.

[0015] Furthermore, a return spring is mounted on the outside of the elastic sac, the lower end of the return spring is fixed in the lower adjustment cap, and the upper end thereof is abutted against the bottom surface of the limit clamping plate, so that the return spring can ensure the rebound and reset of the pressing cap.

[0016] Furthermore, the suction tube is connected to the conical tube through the thermal insulation connecting piece, and the thermal insulation connecting piece is a pipe with a connecting cavity in the middle. The two pipe ends of the thermal insulation connecting piece are respectively connected to the elastic sac and the conical tube, so that the elastic sac and the conical tube do not contact each other, thereby avoiding the melting of the elastic sac when the conical tube is heated; specifically, the inner wall or outer wall of the thermal insulation connecting piece is provided with a protrusion for separating the elastic sac and the conical tube, and the protrusion is a ring structure or a block structure.

[0017] Furthermore, the heating resistance wire is connected to an on / off switch, which is arranged on the lower adjustment cap, so as to easily and quickly turn on or off the heating function; in addition, the heating resistance wire is in contact with the inner wall of the conical tube, which can provide better heat conduction performance.

[0018] Furthermore, adjustment scale lines are provided on the outer wall of the lower adjustment cap in the longitudinal direction, so that when the upper adjustment cap and the lower adjustment cap are screwed to change the height of the adjustment cavity, the height of the adjustment cavity can be accurately determined.

[0019] Furthermore, an annular sleeve surrounding the outer side of the cone tube is provided on the bottom wall of the lower adjusting cap, and a protective sleeve covering the cone tube is screwed on the annular sleeve. The protective sleeve can effectively protect the cone tube made of double-layer glass to prevent the cone tube from being damaged by collision.

[0020] The advantage of the utility model is that the maximum height of the elastic capsule pressed down by the pressing cap can be accurately adjusted by screwing the upper and lower adjusting caps, so that the extrusion height of the elastic capsule can be accurately controlled, ensuring that the suction and injection amount of the cone tube is accurate during each operation, easy operation and good safety; in addition, a heating resistance wire is buried in the cone tube made of double-layer glass, which can continuously heat the liquid sucked in the cone tube, maintain the temperature of the liquid, effectively prevent the liquid sucked into the cone tube from cooling and solidifying, ensure the normal transfer of the liquid, and is suitable for pipetting gel-type culture media that are easy to solidify at room temperature. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a structural diagram of the present utility model.

[0022] Figure 2 yes Figure 1 Enlarged view of part A in the middle.

[0023] Figure 3 It is an axonometric view of the present invention.

[0024] Figure 4 It is an exploded view of the present utility model.

[0025] Figure 5 、 Figure 6 Schematic diagrams of two different forms of thermal insulation connecting pieces. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0027] It should be noted that all directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present invention are only used to explain the relative position relationship, movement status, etc. between the various components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0028] like Figure 1-6 As shown, the gel-type culture medium pipetting device of the present invention includes an upper adjusting cap 1, a lower adjusting cap 2, a pressing cap 3, a conical tube 4 and a suction tube.

[0029] Specifically, the upper adjusting cap 1 is a cylindrical structure, on which is provided an upper chamber 6 with an opening facing downward; the lower adjusting cap 2 is also a cylindrical structure, on which is provided a lower chamber 7 with an opening facing upward, and the lower adjusting cap 2 is coaxially screwed into the upper adjusting cap 1, so that the upper chamber 6 and the lower chamber 7 are connected to form an adjusting chamber, and the height of the lower adjusting cap 2 extending into the upper adjusting cap 1 can be adjusted as needed, thereby changing the height of the adjusting chamber formed by the upper chamber 6 and the lower chamber 7.

[0030] The pressing cap 3 is vertically penetrated on the top wall of the upper adjusting cap 1 and can move up and down along the through-hole; the lower edge of the pressing cap 3 is horizontally turned outward to form a limit card 8 that slides up and down in the upper chamber 6. Driven by the pressing cap 3, the limit card 8 has a first position where it is attached to the top wall of the upper chamber 6, and a second position where it is attached to the edge of the lower adjusting cap 2. When the pressing cap 3 is in the second position, its top end should still be outside the upper adjusting cap 1.

[0031] The conical tube 4 is a double-layer tube with an interlayer cavity in the middle, preferably a double-layer glass tube. The conical tip of the conical tube 4 is vertically fixed to the bottom wall of the lower adjusting cap 2, facing downward. A heating resistor 9 is disposed within the interlayer cavity of the conical tube 4. The heating resistor 9 is in a resistance heating mode and is connected to a power supply and an on / off switch 10. The on / off switch 10 controls whether the heating resistor 9 is turned on or off. In addition, the on / off switch 10 is a temperature-controlled switch with a temperature control function. It is installed on the side wall of the lower adjusting cap 2 near the bottom, making it easy and quick to turn the heating resistor 9 on and off. When the on / off switch 10 is controlled to turn on the heating resistor 9 and its temperature reaches above 40°C, pipetting can begin. To prevent thermal runaway, the heating temperature can be controlled by the temperature control function of the on / off switch 10, preferably below 80°C, to ensure that the gel-forming culture medium sample liquid does not solidify. In order to provide better heat conduction performance, the heating resistance wire 9 is in contact with the inner wall of the conical tube 4. It is more preferred to wind the heating resistance wire 9 around the inner wall of the conical tube 4 to better maintain the temperature of the chamber in which the conical tube attracts liquid. Of course, it is also possible to fill the interlayer cavity of the conical tube 4 with heat-conducting oil, so that the temperature of the conical tube 4 can be better maintained when the heating resistance wire 9 is not in contact with the conical tube 4. However, it is necessary to set the electrode of the external power supply of the heating resistance wire 9 outside the interlayer of the conical tube 4, and at the same time, it is necessary to maintain the heating oil in the interlayer of the conical tube 4 from leaking, which puts higher requirements on the sealing of the conical tube 4.

[0032] The suction tube is an elastic capsule 5 arranged in the regulating cavity. The elastic capsule 5 can be an elastic rubber cap made of rubber material. Of course, the elastic capsule 5 is preferably a bellows telescopic tube formed by connecting foldable corrugated sheets along the folding and telescopic direction. The elastic capsule 5 is provided with an opening and is sealed and connected with the cone tube 4 through a heat-insulating connecting piece 17. The pressing cap 3 can exert force on the elastic capsule 5. The heat-insulating connecting piece 17 is made of heat-insulating material. Zirconia insulating ceramic material or polyetheretherketone (PEEK) material can be selected. It can avoid deformation of the elastic capsule 5 caused by the increase in the temperature of the cone tube 4 and the heat conduction effect, thereby avoiding the reduction in the life of the entire pipetting device or the deviation in the amount of liquid suction caused by reduced sealing. Specifically, Figure 5 、 6 As shown, the heat-insulating connecting piece 17 is a pipe with a connecting cavity in the middle. The two pipe ends of the heat-insulating connecting piece 17 are connected to the elastic bladder 5 and the cone tube 4 respectively, so that the elastic bladder 5 and the cone tube 4 do not contact each other. In order to facilitate the installation of the heat-insulating connecting piece 17, the elastic bladder 5 and the cone tube 4, a protrusion for separating the elastic bladder 5 and the cone tube 4 can be provided on the inner wall or outer wall of the heat-insulating connecting piece 17. The protrusion can be an outer protrusion 18 (such as Figure 5 As shown), it can also be an inner protrusion 19 (as shown) provided on the inner wall of the thermal insulation connecting piece 17 Figure 6 As shown); when the protrusion is an external protrusion 18 provided on the outer wall of the thermal insulation connecting piece 17, the inner diameter of the lower end of the elastic sac 5 and the inner diameter of the upper end of the tapered tube 4 just match the outer diameter of the thermal insulation connecting piece 17, so that the elastic sac 5 and the tapered tube 4 can be respectively sleeved on the two ends of the thermal insulation connecting piece 17; when the protrusion is an internal protrusion 19 provided on the inner wall of the thermal insulation connecting piece 17, the outer diameter of the lower end of the elastic sac 5 and the outer diameter of the upper end of the tapered tube 4 just match the inner diameter of the thermal insulation connecting piece 17, so that the two ends of the thermal insulation connecting piece 17 can be respectively sleeved on the elastic sac 5 and the tapered tube 4. In addition, the protrusion can be an annular structure or a block structure. When the protrusion is a block structure, it is preferably provided as two or more with the same height to adapt to the tube openings of the elastic sac 5 and the tapered tube 4 with flat openings.

[0033] During use, the upper and lower adjusting caps 1 and 2 are screwed in accordance with the desired amount of gelled culture medium sample liquid to be aspirated, thereby precisely adjusting the height of the adjusting chamber formed by the communication between the upper and lower chambers 6 and 7 (i.e., precisely adjusting the downward pressure of the pressing cap 3). The conical tube 4 is then inserted into the container containing the gelled culture medium sample liquid. The pressing cap 3 is pressed downward to the bottom, causing it to move from a first position to a second position. This compresses the elastic bladder 5, causing it to contract. When the pressing cap 3 is released, the elastic bladder 5, constructed as a bellows or rubber cap, automatically rebounds, creating a suction force that draws the gelled culture medium sample liquid into the conical tube 4. The pressing cap 3 is then pressed downward again to the bottom, allowing the gelled culture medium sample liquid aspirated from the conical tube 4 to be injected into another container, completing the pipetting operation. Because the distance of each press of the pressing cap 3 is fixed, the amount of liquid aspirated and injected into the conical tube 4 is also precisely fixed, achieving quantitative pipetting with ease and safety. In addition, during the operation of aspirating and injecting liquid, the heating resistance wire 9 must always be turned on to continuously heat the gelled culture medium sample liquid in the aspirated conical tube 4, maintain the temperature of the gelled culture medium sample liquid, and effectively prevent the gelled culture medium sample liquid in the aspirated conical tube 4 from cooling and solidifying, thereby ensuring normal transfer.

[0034] Furthermore, in order to enable the pressing cap 3 to stably press the elastic capsule 5 downward, the center of the upper end surface of the elastic capsule 5 can be recessed to form a positioning groove 11, and a positioning protrusion 12 that is adapted to and engaged with the positioning groove 11 can be provided on the pressing cap 3, so that the upper end of the elastic capsule 5 can be firmly attached to the pressing cap 3, ensuring that when the pressing cap 3 pushes the elastic capsule 5, the elastic capsule 5, the pressing cap 3 and the conical tube 4 can always be kept coaxially arranged.

[0035] Furthermore, in order to prevent the rebound force of the elastic capsule 5 from making it difficult to push the pressing cap 3 upward to rebound, a return spring 13 can be installed on the outside of the elastic capsule 5. The lower end of the return spring 13 is fixed in the lower adjusting cap 2, and the upper end thereof is abutted against the bottom surface of the limiting clamping plate 8, thereby ensuring the rebound and reset of the pressing cap 3 by the return spring 13; at the same time, when the upper adjusting cap 1 and the lower adjusting cap 2 are screwed to shorten the height of the adjusting cavity, it will only slightly squeeze the return spring 13, so that the rebound force of the return spring 13 is slightly increased, which will not affect the quantitative liquid absorption of the entire device.

[0036] Furthermore, in order to accurately observe the amount of liquid absorbed by pressing the pressing cap 3 once, an adjustment scale line 14 can be set longitudinally on the outer wall of the lower adjustment cap 2, so that when the upper adjustment cap 1 and the lower adjustment cap 2 are screwed to change the height of the adjustment cavity, the height of the adjustment cavity can be accurately determined by adjusting the scale line 14, so that the height of the elastic capsule 5 pressed downward by the pressing cap 3 can be accurately determined, thereby realizing quantitative liquid absorption of the conical tube 4.

[0037] Furthermore, since the conical tube 4 is made of double-layer glass, in order to prevent the conical tube 4 from being damaged by bumps, an annular sleeve 15 surrounding the outer side of the conical tube 4 can be provided on the bottom wall of the lower adjusting cap 2, and a protective cover 16 covering the conical tube 4 is screwed onto the annular sleeve 15. The protective cover 16 is removed only when the conical tube 4 is used for aspiration, thereby effectively protecting the conical tube 4.

Claims

1. A gel-type culture medium pipetting device, characterized in that: include An upper adjusting cap having an upper chamber with a downward opening; A lower adjusting cap having a lower chamber with an upward opening, wherein the lower adjusting cap is coaxially screwed into the upper adjusting cap so that the upper chamber and the lower chamber are connected to form an adjusting chamber; A pressing cap is inserted into the top wall of the upper adjusting cap, and the lower edge of the pressing cap is horizontally turned outward to form a limiting card that slides up and down along the upper chamber; The conical tube is a double-layer tube vertically fixed on the bottom wall of the lower regulating cap and having an interlayer cavity in the middle. A heating resistance wire is provided in the interlayer cavity of the conical tube; The suction tube is an elastic capsule arranged in the regulating cavity. The elastic capsule is provided with an opening which is sealed and communicated with the cone tube. The pressing cap applies force to the elastic capsule.

2. The gelled culture medium pipetting device according to claim 1, characterized in that: The elastic capsule is a rubber cap or a corrugated telescopic tube.

3. The gelled culture medium pipetting device according to claim 1 or 2, characterized in that: The center of the upper end surface of the elastic sac is concave to form a positioning groove, and the pressing cap is provided with a positioning protrusion adapted to engage with the positioning groove. The pressing cap, the cone tube and the elastic sac are coaxially arranged.

4. The gelled culture medium pipetting device according to claim 3, characterized in that: A return spring is sleeved on the outside of the elastic bag body, the lower end of the return spring is fixed in the lower adjustment cap, and the upper end of the return spring is attached to the bottom surface of the limit clamping plate.

5. The gelled culture medium pipetting device according to claim 1 or 2, characterized in that: The suction tube is connected to the cone tube via a heat-insulating connecting piece. The heat-insulating connecting piece is a pipe with a connecting cavity in the middle. Two pipe openings of the heat-insulating connecting piece are respectively connected to the elastic sac and the cone tube.

6. The gelled culture medium pipetting device according to claim 5, characterized in that: The inner wall or outer wall of the thermal insulation connecting piece is provided with a protrusion for separating the elastic bag and the cone tube, and the protrusion is an annular structure or a block structure.

7. The gelled culture medium pipetting device according to claim 1, characterized in that: The heating resistance wire is connected to an on-off switch, and the on-off switch is arranged on the lower adjusting cap.

8. The gelled culture medium pipetting device according to claim 1 or 7, characterized in that: The heating resistance wire is in contact with the inner wall of the conical tube.

9. The gelled culture medium pipetting device according to claim 1, characterized in that: Adjustment scale lines are arranged on the outer wall of the lower adjustment cap along the longitudinal direction.

10. The gelled culture medium pipetting device according to claim 1, characterized in that: An annular sleeve surrounding the outer side of the cone tube is provided on the bottom wall of the lower adjusting cap, and a protective sleeve covering the cone tube is screwed on the annular sleeve.

Images