Desalination column fixing support

Abstract

The utility model provides a desalination column fixing support which comprises a first sleeve and a second sleeve, the axis of the first sleeve and the axis of the second sleeve are collinear, the aperture of the first sleeve is larger than that of the second sleeve, and the second sleeve is arranged at one end of the first sleeve and fixedly connected with the first sleeve through a horizontal plate; wherein the first sleeve is used for being connected with a tube opening of a centrifugal tube in a matched mode, the first sleeve is combined with the horizontal plate to support a tube body part of the desalination column, and a needle head part of the desalination column penetrates through the second sleeve to stretch into the centrifugal tube. The desalting column can be vertically placed at the tube opening of the centrifugal tube through the fixing bracket, and the needle tube part is kept far away from the tube bottom of the centrifugal tube, so that automatic falling and sample loading of a peptide fragment are realized, the desalting column does not need to be held manually in the whole process, the occupation of automatic desalting equipment is also avoided, and the overall efficiency of an experiment is greatly improved.

Description

Technical Field

[0001] The utility model relates to the technical field of protein spectrum sample preparation, in particular to a desalting column fixing bracket. Background Art

[0002] In the field of proteomics research and mass spectrometry sample preparation, after protein samples are enzymatically cleaved into peptide samples, they need to be desalted to remove impurities such as salt and other small molecules in the samples. As a porous material, C18 reversed-phase resin has become an indispensable desalting tool medium due to its excellent binding ability and high recovery efficiency. Currently, C18 desalting columns of various specifications are widely used in sample desalting, concentration of target peptides, and processing of biological samples, playing a vital role. In the proteomics technology platform, large-scale clinical samples need to be processed simultaneously, so laboratories are often equipped with automated high-throughput C18 desalting kit instruments, which aim to simplify the desalting operation process through machine operation and improve the efficiency of sample processing.

[0003] However, when special and precious samples require meticulous processing, researchers must manually handle samples to minimize experimental waste and monitor progress in real time. However, the original C18 desalting column lacks a support bracket, requiring researchers to hold the sample in their hand throughout the desalting process while the sample is filtered, and researchers cannot work on more than two samples simultaneously. If a small number of samples occupy the automated desalting equipment for extended periods, this will affect the use of other large-scale samples, resulting in instrument idleness and loss. Furthermore, automated desalting instruments have low precision and are difficult to observe during the experiment, making it difficult to detect potential experimental errors and easily leading to sample loss. Utility Model Content

[0004] Based on this, the purpose of the present invention is to provide a desalting column fixing bracket for solving the technical problems mentioned in the above background technology.

[0005] The utility model provides a desalting column fixing bracket, comprising a first sleeve and a second sleeve whose axis lines are collinear, wherein the aperture of the first sleeve is larger than that of the second sleeve, and the second sleeve is arranged at one end of the first sleeve and is connected and fixed to the first sleeve via a horizontal plate;

[0006] The first sleeve is used to match the nozzle of the centrifuge tube, and the first sleeve is combined with the horizontal plate to form a support for the body of the desalting column, while the needle of the desalting column passes through the second sleeve and extends into the centrifuge tube.

[0007] Furthermore, in the desalting column fixing bracket, the first sleeve and the centrifuge tube are connected with each other by interference fit.

[0008] Furthermore, in the desalting column fixing bracket, a limiting ring is provided on the outer wall of the first sleeve.

[0009] Furthermore, in the desalting column fixing bracket, the diameter of the limiting ring gradually decreases from top to bottom.

[0010] Furthermore, in the desalting column fixing bracket, the peripheral annular array of the limiting ring has a plurality of elastic clamping arms, and the ends of the elastic clamping arms are provided with clamping heads.

[0011] Furthermore, the desalting column fixing bracket has a chuck wrapped with an anti-slip film.

[0012] Furthermore, in the desalting column fixing bracket, a plurality of spring pieces are provided in a circular array on the inner wall of the first sleeve, and the spring pieces have an arched surface protruding relative to the inner wall of the first sleeve.

[0013] Furthermore, in the desalting column fixing bracket, the outer wall of the first sleeve is provided with a frosted area.

[0014] Compared with the prior art, the beneficial effects of the present invention are:

[0015] The desalting column can be placed vertically at the mouth of the centrifuge tube through a fixed bracket, and the needle part is kept away from the bottom of the centrifuge tube, realizing the automatic falling and loading of peptides. There is no need to manually hold the desalting column throughout the process, and it also avoids the occupation of automated desalting equipment, greatly improving the overall efficiency of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a three-dimensional diagram of the desalting column fixing bracket in the first embodiment of the present utility model from the first viewing angle;

[0017] Figure 2 This is a three-dimensional view of the desalting column fixing bracket in the first embodiment of the present utility model from a second viewing angle;

[0018] Figure 3 This is a schematic diagram of the assembly state of the desalting column fixing bracket, the desalting column and the centrifuge tube in the first embodiment of the present utility model;

[0019] Figure 4 This is a schematic diagram of the desalting column fixing bracket, the desalting column and the centrifuge tube in the first embodiment of the present invention in an exploded state;

[0020] Figure 5 This is a three-dimensional diagram of the desalting column fixing bracket in the second embodiment of the present utility model from the first viewing angle;

[0021] Figure 6 This is a three-dimensional diagram of the desalting column fixing bracket in the second embodiment of the present utility model from a second viewing angle;

[0022] Figure 7This is a schematic diagram of the assembly state of the desalting column fixing bracket, the desalting column and the centrifuge tube in the second embodiment of the present invention;

[0023] Description of main component symbols:

[0024] 11. First sleeve; 12. Second sleeve; 13. Horizontal plate; 14. Frosted area; 21. Limiting ring; 22. Elastic clamp arm; 23. Clamp; 30. Shrapnel; 40. Centrifuge tube; 50. Desalting column; 51. Tube body; 52. Needle part.

[0025] The following specific embodiments will further illustrate the present invention in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0026] To facilitate understanding of the present invention, a more comprehensive description of the present invention will be provided below with reference to the accompanying drawings. The drawings illustrate several embodiments of the present invention. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a more thorough and comprehensive understanding of the present invention.

[0027] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be an intermediate element. When an element is referred to as being "connected to" another element, it may be directly connected to the other element or there may be an intermediate element. The terms "vertical," "horizontal," "left," "right," and similar expressions used herein are for illustrative purposes only.

[0028] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention pertains. The terms used herein in the specification of this invention are intended only to describe specific embodiments and are not intended to limit the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0029] First embodiment

[0030] See also Figures 1 to 4 The desalting column fixing bracket of the present invention comprises a first sleeve 11 and a second sleeve 12 whose axis lines are collinear. The aperture of the first sleeve 11 is larger than that of the second sleeve 12. The second sleeve 12 is arranged at one end of the first sleeve 11 and is connected and fixed thereto through a horizontal plate 13.

[0031] The first sleeve 11 is used to cooperate with the tube mouth of the centrifuge tube 40. The first sleeve 11 is combined with the horizontal plate 13 to form a support for the tube body 51 of the desalting column 50, and the needle part 52 of the desalting column 50 passes through the second sleeve 12 and extends into the centrifuge tube 40.

[0032] The desalting column 50 can be placed vertically at the mouth of the centrifuge tube 40 through a fixed bracket, and the needle part is kept away from the bottom of the centrifuge tube 40, thereby realizing the automatic falling and loading of the peptide segment. There is no need to manually hold the desalting column 50 throughout the process, and it also avoids occupying the automated desalting equipment, greatly improving the overall efficiency of the experiment.

[0033] The first sleeve 11 and the second sleeve 12 are both cylindrical in shape to fit the contours of the tube body 51 and the needle portion 52 of the desalting column 50 , wherein the aperture of the second sleeve 12 is slightly larger than the needle portion 52 of the desalting column 50 , so that the needle portion 52 can pass through the second sleeve 12 and extend into the centrifuge tube 40 .

[0034] In this embodiment, the tube diameter of the first sleeve 11 is the same as the pore diameter of the centrifuge tube 40, so the first sleeve 11 and the centrifuge tube 40 are connected by interference fit. One fixed bracket can only be used for a centrifuge tube 40 of a specific capacity. For centrifuge tubes 40 of different capacities (such as 1.5ml, 2ml, 5ml, etc.), it is necessary to design a plurality of fixed brackets of different sizes.

[0035] In this embodiment, the first sleeve 11, the second sleeve 12 and the horizontal plate 13 are all made of high-strength materials such as polypropylene (PP) or polyethylene (PE), which have good chemical stability and temperature resistance.

[0036] See Figure 1 The outer wall of the first sleeve 11 is provided with a frosted area 14, which can be used to mark the sample name, helping the experimenter to find the target sample quickly and accurately.

[0037] In practical application, the experimental steps are as follows:

[0038] 1. Take an appropriate amount of digested peptide sample and dissolve the sample in 1 mL of 0.1% (vol / vol) TFA;

[0039] 2. Activate the desalting column: Assemble the desalting column 50, the fixing bracket, and the centrifuge tube 40 from top to bottom. At this time, the desalting column 50 is fixed vertically above the 15 mL centrifuge tube 40. Pretreat the desalting column 50 with 3 mL of 100% (v / v) ACN / 0.1% (v / v) TFA, and discard the waste liquid.

[0040] 3. Equilibrate the desalting column: Equilibrate the column with 3 × 3 mL of 0.1% (vol / vol) TFA and discard the waste solution;

[0041] 4. Loading the sample: Place the desalting column 50 and the fixed support assembly above the Eppendoff tube in which the sample is dissolved, and load the dissolved sample supernatant into the desalting column 50, allowing the sample to fall automatically. Repeat this process three times.

[0042] 5. Washing the sample: Place the desalting column 50 and the fixed support assembly above the centrifuge tube 40 used to collect the waste liquid, and wash (desalt) the sample with 3 mL of 0.1% (vol / vol) TFA;

[0043] 6. Elution of sample: Place the desalting column 50 and the fixed support assembly on a new centrifuge tube 40, and elute the sample from the C18 desalting column 50 with 3 × 1 mL of 75% (vol / vol) ACN / 0.1% (vol / vol) TFA;

[0044] 7. Dry the sample by vacuum centrifugation;

[0045] 8. The proteomic samples were analyzed using an Orbitrap Fusion mass spectrometer coupled with a QE ultra-high pressure liquid chromatography pump. The peptides were matched to proteins using MASCOT software, and the raw data were searched against the Uniprot database for different species.

[0046] In summary, the desalting column fixing bracket in the above embodiment of the present invention allows the desalting column 50 to be placed vertically at the mouth of the centrifuge tube 40 through the fixing bracket, and keeps the needle part away from the bottom of the centrifuge tube 40, thereby realizing the automatic falling and loading of the peptide segment. There is no need to manually hold the desalting column 50 throughout the process, and it also avoids the occupation of the automated desalting equipment, greatly improving the overall efficiency of the experiment.

[0047] Second embodiment

[0048] See Figures 5 to 7The desalting column fixing bracket in the second embodiment of the present invention differs from the desalting column fixing bracket in the first embodiment in that a retaining ring 21 is provided on the outer wall of the first sleeve 11. The retaining ring 21 can rest on the edge of the nozzle of the centrifuge tube 40 to achieve docking of the first sleeve 11 at the nozzle of the centrifuge tube 40. This allows a single fixing bracket to accommodate a variety of centrifuge tubes 40 of different capacities. It is understood that in this embodiment, since the first sleeve 11 is suspended at the nozzle of the centrifuge tube 40 by the retaining ring 21, the diameter of the first sleeve 11 does not need to be designed to be the same as the aperture of the centrifuge tube 40, but can be designed to be smaller than the aperture of the centrifuge tube 40. This allows the first sleeve 11 to be inserted into centrifuge tubes 40 of different capacities, greatly improving the adaptability of the fixing bracket. Specifically, the aperture of the centrifuge tubes 40 of different capacities should be larger than the diameter of the first sleeve 11 but smaller than the outer diameter of the retaining ring 21.

[0049] Furthermore, the diameter of the retaining ring 21 gradually decreases from top to bottom, so that its outer wall forms a conical surface. It is understandable that by designing the outer wall of the retaining ring 21 as a conical surface, the conical surface of the retaining ring 21 can gradually cling to the inner wall of the centrifuge tube 40 as the first sleeve 11 is inserted deeper, ensuring that the central axes of the first sleeve 11 and the centrifuge tube 40 are collinear, effectively improving the stability of the connection between the two.

[0050] See Figure 5 and Figure 7 The retaining ring 21 has a plurality of elastic clamping arms 22 arranged in an annular array around its periphery. The ends of the elastic clamping arms 22 are provided with chucks 23. Specifically, in this embodiment, there are three elastic clamping arms 22. When the first sleeve 11 is inserted into the centrifuge tube 40 and the retaining ring 21 is tightly fitted against the inner wall of the centrifuge tube 40, the chucks 23 of the three elastic clamping arms 22 can tightly embrace the outer wall of the centrifuge tube 40, further enhancing the stability of the connection between the fixing bracket and the centrifuge tube 40.

[0051] Furthermore, the chuck 23 is wrapped with an anti-skid film to increase the friction between the chuck 23 and the outer wall of the centrifuge tube 40 to prevent the two from slipping and becoming loose.

[0052] See Figure 6The inner wall of the first sleeve 11 is provided with a plurality of spring clips 30 in a circular array. The spring clips 30 have an arched surface that protrudes relative to the inner wall of the first sleeve 11. The design of the spring clips 30 allows a small-sized desalination column 50 to be effectively fixed when inserted into the first sleeve 11. Specifically, in this embodiment, the number of spring clips 30 is three. When the desalination column 50 is inserted into the first sleeve 11, the arched surface of the spring clips 30 can closely fit the tube portion 51 of the desalination column 50 and rebound accordingly according to the size of the tube portion 51, thereby allowing a small-sized desalination column 50 to be stably fixed in the first sleeve 11.

[0053] Throughout this specification, reference to terms such as "one embodiment," "some embodiments," "examples," "specific examples," or "some examples" means that a specific feature, structure, material, or characteristic described in conjunction with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0054] The above-described embodiments merely represent several implementation methods of the present invention. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art would be able to make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements fall within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be determined by the appended claims.

Claims

1. A desalting column fixing bracket, characterized in that: The invention comprises a first sleeve and a second sleeve whose axis lines are collinear, wherein the aperture of the first sleeve is larger than the aperture of the second sleeve, and the second sleeve is arranged at one end of the first sleeve and is connected and fixed to the first sleeve via a horizontal plate; The first sleeve is used to match the nozzle of the centrifuge tube, and the first sleeve is combined with the horizontal plate to form a support for the body of the desalting column, while the needle of the desalting column passes through the second sleeve and extends into the centrifuge tube.

2. The desalting column fixing bracket according to claim 1, characterized in that: The first sleeve and the centrifuge tube are connected with each other in an interference fit manner.

3. The desalting column fixing bracket according to claim 1, characterized in that: A limiting ring is provided on the outer wall of the first sleeve.

4. The desalting column fixing bracket according to claim 3, characterized in that: The diameter of the limiting ring gradually decreases from top to bottom.

5. The desalting column fixing bracket according to claim 3, characterized in that: The peripheral annular array of the limiting ring is provided with a plurality of elastic clamping arms, and the ends of the elastic clamping arms are provided with clamping heads.

6. The desalting column fixing bracket according to claim 5, characterized in that: The chuck is wrapped with an anti-slip film.

7. The desalting column fixing bracket according to claim 3, characterized in that: The inner wall of the first sleeve is provided with a plurality of spring pieces in an annular array. The spring pieces have an arched surface that protrudes relative to the inner wall of the first sleeve.

8. The desalting column fixing bracket according to claim 1, characterized in that: The outer wall of the first sleeve is provided with a frosted area.

Images