Single-cell suspension blowing and beating gun head
By introducing a buffer rubber ring and a conical dispersion block into the single-cell suspension pipette tip, the problem of poor buffering effect of the single-cell suspension was solved, and the cell survival rate was significantly improved.
Patent Information
- Application Number
- CN202422791077.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-15
- Publication Date
- 2025-09-16
- Estimated Expiration
- 2034-11-15
AI Technical Summary
In the prior art, single-cell suspensions rely on non-Newtonian fluids for buffering, but this has a poor buffering effect, resulting in high cell damage or rupture rates.
The buffer rubber ring and conical dispersion block design are used to reduce the impact of the single-cell suspension on the barrel wall through the buffer rubber ring, and the conical dispersion block is used to disperse the single-cell suspension and reduce the flow rate.
It effectively reduces the collision between the single cell suspension and the barrel wall and improves the survival rate of cells.
Smart Images

Figure CN223337362U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of pipette tips, in particular to a single cell suspension blowing and beating pipette tip. Background Art
[0002] A pipette is a type of liquid transfer device commonly used to transfer liquids when preparing single-cell suspensions. Different specifications of pipettes are used with corresponding pipette tips. The preparation of single-cell suspensions is an important experimental method for cytological research. To obtain high-purity single-cell suspensions, researchers need to control grinding, filtration, and environmental factors such as temperature during the preparation process.
[0003] As disclosed in the publication (announcement) number: CN219463451U, a single-cell suspension blowing gun head is disclosed, comprising a gun barrel and a conical suction tip, characterized in that: a wall groove is provided on the inner wall of the gun barrel, a capsule is provided in the wall groove, and there are several capsules, which are distributed in a ring shape with the axis of the gun barrel as the center, and each of the capsules is filled with a non-Newtonian fluid. A flexible buffer section is provided on the gun barrel, and the wall groove and the capsule are both arranged on the inner wall of the flexible buffer section. An elastic connecting section is provided at the connection between the conical suction tip and the gun barrel, and several gap grooves are provided on the conical suction tip and the elastic connecting section, and the gap grooves are distributed in a ring shape with the axis of the conical suction tip as the center. The utility model can effectively slow down the impact between the cells and the inner wall of the gun head during the blowing of the single-cell suspension, avoid cell damage or rupture, and improve cell survival rate.
[0004] Although the above technical solution solves the corresponding technical problem, it still has the following defects:
[0005] The above technical solution relies solely on the non-Newtonian fluid on the tube wall to buffer the single-cell suspension. However, it is well known that when a non-Newtonian fluid is hit at high speed, its viscosity will increase rapidly and become hard. Therefore, it is not very effective in buffering the single-cell suspension. Utility Model Content
[0006] The purpose of the utility model is to provide a single-cell suspension blowing gun tip, which not only reduces the collision between the single-cell suspension and the barrel wall by arranging a buffer rubber ring, thereby providing good protection for the single-cell suspension, but also, when the single-cell suspension is sucked up through the conical gun tip, the single-cell suspension can be dispersed under the action of the conical dispersion block, thereby avoiding a high flow rate of the single-cell suspension, thereby providing good buffering protection for the single-cell suspension, and thus greatly reducing cell damage or rupture, thereby improving cell survival rate.
[0007] In order to achieve the above-mentioned purpose, the main technical solutions adopted by this utility model include:
[0008] A single cell suspension pipetting tip, comprising:
[0009] The pipette body is provided with a detachable suction component at the bottom of the pipette body, and the suction component includes a gun barrel, the top of the gun barrel is fixedly connected to a pipette connecting seat, and the bottom of the pipette body is fixedly connected to a pipette connecting head corresponding to the position of the pipette connecting seat and used in conjunction with the pipette connecting seat.
[0010] The above-mentioned single-cell suspension blowing gun tip, wherein the outside of the pipette connector is provided with an external thread, and the inner wall of the pipette connector is provided with an internal thread that cooperates with the external thread.
[0011] The above-mentioned single cell suspension blowing gun tip, wherein the bottom of the gun barrel is fixedly connected to a conical gun tip.
[0012] The above-mentioned single cell suspension blowing gun tip, wherein a buffer rubber ring is fixedly connected to the inside of the gun barrel.
[0013] The above-mentioned single cell suspension blowing gun tip, wherein a dispersion component for dispersing the single cell suspension is also installed inside the gun barrel and near the conical gun tip.
[0014] The above-mentioned single-cell suspension blowing gun tip, wherein the dispersion component includes a mounting rod fixedly connected to the inner wall of the gun barrel, and a conical dispersion block is fixedly connected to the bottom of the mounting rod.
[0015] The utility model has at least the following beneficial effects:
[0016] In the utility model, a single-cell suspension blowing gun head is realized, which not only reduces the collision between the single-cell suspension and the barrel wall by arranging a buffer rubber ring, thereby playing a good protective role for the single-cell suspension, but also, when the single-cell suspension is sucked up by the conical gun head, the single-cell suspension can be dispersed by the action of the conical dispersion block, thereby avoiding a high flow rate of the single-cell suspension, thereby playing a good buffering protection role for the single-cell suspension, and thus greatly reducing the damage or rupture of the cells, thereby improving the cell survival rate. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:
[0018] Figure 1 This is a schematic diagram of the structure of the single cell suspension pipetting tip of the utility model;
[0019] Figure 2 This is a schematic diagram of the cross-sectional structure of the single cell suspension pipetting tip of the utility model;
[0020] Figure 3 This is a schematic diagram of the structure of the aspiration component in the single cell suspension pipetting gun tip of the present invention;
[0021] Figure 4 This is a schematic diagram of the structure of the pipette body in the single cell suspension pipetting gun tip of the present invention;
[0022] Figure 5 This is a schematic diagram of the structure of the buffer rubber ring in the single cell suspension pipetting gun tip of the utility model;
[0023] Figure 6 This is a schematic diagram of the structure of the dispersion component in the single cell suspension pipetting gun tip of the present invention;
[0024] Figure 7 This is a schematic structural diagram of another perspective of the dispersion component in the single cell suspension pipetting gun tip of the present invention.
[0025] Description of Figure Numbers:
[0026] 1. Pipette body; 2. Suction assembly;
[0027] 101. Pipette connector; 1011. External thread;
[0028] 201, gun barrel; 2011, tapered gun head;
[0029] 202, pipette connection seat; 2021, internal thread; 2022, sealing ring;
[0030] 203, buffer rubber ring;
[0031] 204, decentralized components;
[0032] 2041. Mounting rod; 2042. Conical dispersion block. DETAILED DESCRIPTION
[0033] The following will describe the implementation methods of the present application in detail with reference to the accompanying drawings and examples, so that the implementation process of how the present application applies technical means to solve technical problems and achieve technical effects can be fully understood and implemented accordingly.
[0034] Please refer to Figures 1 to 7As shown, a single cell suspension pipetting gun tip provided in this embodiment includes: a pipette body 1, a detachable suction assembly 2 is provided at the bottom of the pipette body 1, the suction assembly 2 includes a barrel 201, the top of the barrel 201 is fixedly connected to a pipette connection seat 202, and a pipette connector 101 that cooperates with the pipette connection seat 202 is fixedly connected to the bottom of the pipette body 1 and corresponds to the position of the pipette connection seat 202;
[0035] The single-cell suspension blowing gun tip using the above-mentioned technical solution can not only reduce the collision between the single-cell suspension and the wall of the barrel 201 by setting the buffer rubber ring 203, thereby providing good protection for the single-cell suspension, but also, when the single-cell suspension is sucked up through the conical gun tip 2011, the single-cell suspension can be dispersed under the action of the conical dispersion block 2042, thereby avoiding a high flow rate of the single-cell suspension, and thus providing good buffering protection for the single-cell suspension, thereby greatly reducing cell damage or rupture, thereby improving cell survival rate.
[0036] Please refer to Figures 1 to 7 As shown, the outside of the pipette connector 101 is provided with an external thread 1011, and the inner wall of the pipette connector 202 is provided with an internal thread 2021 that cooperates with the external thread 1011. The convenience of disassembly and assembly of the pipette connector 101 and the pipette connector 202 is increased by setting the external thread 1011 and the internal thread 2021.
[0037] Please refer to Figures 1 to 7 As shown, a conical gun head 2011 is fixedly connected to the bottom of the gun barrel 201.
[0038] Please refer to Figures 1 to 7 As shown, a buffer rubber ring 203 is fixedly connected to the interior of the gun barrel 201. The buffer rubber ring 203 can reduce the impact between the single cell suspension and the wall of the gun barrel 201, thereby providing good protection for the single cell suspension.
[0039] Please refer to Figures 1 to 7 As shown, a dispersion component 204 for dispersing a single cell suspension is installed inside the gun barrel 201 and near the conical gun tip 2011 .
[0040] Please refer to Figures 1 to 7 As shown, the dispersion assembly 204 includes a mounting rod 2041 fixedly connected to the inner wall of the barrel 201. A conical dispersion block 2042 is fixedly connected to the bottom of the mounting rod 2041. The conical dispersion block 2042 disperses the single cell suspension to prevent the single cell suspension from flowing too fast, thereby providing good buffering protection for the single cell suspension.
[0041] A sealing ring 2022 is fixedly connected to the inner wall of the pipette connection base 202 . The sealing ring 2022 improves the sealing performance when the pipette connection head 101 and the pipette connection base 202 are connected.
[0042] The working principle of the present invention is as follows: when a single-cell suspension is sucked up through the conical gun tip 2011, not only can the collision between the single-cell suspension and the wall of the gun barrel 201 be reduced by providing the buffer rubber ring 203, thereby providing good protection for the single-cell suspension, but the single-cell suspension sucked up through the conical gun tip 2011 will first collide with the conical gun tip 2011, and under the conical dispersion effect of the conical gun tip 2011, the single-cell suspension will be evenly dispersed, thereby avoiding a high flow rate of the single-cell suspension, thereby providing good buffering protection for the single-cell suspension, and thus greatly reducing cell damage or rupture, thereby improving cell survival rate.
[0043] The above description shows and describes several preferred embodiments of the present invention. However, as mentioned above, it should be understood that the present invention is not limited to the form disclosed herein and should not be construed as excluding other embodiments. Instead, the present invention can be used in various other combinations, modifications, and environments and can be modified within the scope of the present invention through the above teachings or the technology or knowledge in the relevant field. Modifications and variations made by those skilled in the art without departing from the spirit and scope of the present invention are intended to be protected by the claims appended hereto.
Claims
1. A single cell suspension pipetting gun tip, comprising a pipette body (1), characterized in that: A detachable suction assembly (2) is provided at the bottom of the pipette body (1), and the suction assembly (2) includes a gun barrel (201), the top of the gun barrel (201) is fixedly connected to a pipette connection seat (202), and a pipette connection head (101) that cooperates with the pipette connection seat (202) is fixedly connected to the bottom of the pipette body (1) and at a position corresponding to the pipette connection seat (202).
2. A single cell suspension pipetting gun tip according to claim 1, characterized in that: The exterior of the pipette connector (101) is provided with an external thread (1011), and the inner wall of the pipette connector (202) is provided with an internal thread (2021) that cooperates with the external thread (1011).
3. A single cell suspension pipetting gun tip according to claim 2, characterized in that: The bottom of the gun barrel (201) is fixedly connected with a conical gun head (2011).
4. A single cell suspension pipetting gun tip according to claim 3, characterized in that: A buffer rubber ring (203) is fixedly connected to the interior of the gun barrel (201).
5. A single cell suspension pipetting gun tip according to claim 4, characterized in that: A dispersion component (204) for dispersing a single cell suspension is also installed inside the gun barrel (201) and near the conical gun head (2011).
6. A single cell suspension pipetting gun tip according to claim 5, characterized in that: The dispersion assembly (204) comprises a mounting rod (2041) fixedly connected to the inner wall of the gun barrel (201), and a conical dispersion block (2042) is fixedly connected to the bottom of the mounting rod (2041).
Citation Information
Patent Citations
Single-cell suspension blowing and beating gun head
CN219463451U