Woody plant tissue culture stem explant preparation device

Abstract

The utility model relates to the technical field of stem section explant preparation, in particular to a woody plant tissue culture stem section explant preparation device, and solves the problems that in the prior art, the stem section manufacturing process is tedious in operation, the working process is long in time consumption, and the stem section manufacturing efficiency is influenced. A woody plant tissue culture stem explant preparation device comprises a processing table and a conveying belt located at the top of the processing table, supporting rods are fixedly connected to the two sides of the conveying belt, blades are arranged on the surfaces of the supporting rods through adjusting mechanisms, a positioning mechanism is arranged on the surface of the conveying belt, and a cutting table is fixedly connected to the top of the processing table; the top of the cutting table is fixedly connected with an L-shaped cutting frame, and a plurality of cutting knives are arranged on the surface of the cutting frame through an extrusion mechanism. According to the utility model, the preparation efficiency of the explant of the tissue culture stem section is greatly improved, the exposure time of branches in the air is shortened, and the quality of starting culture is ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of stem segment explant preparation, in particular to a device for preparing woody plant tissue culture stem segment explants. Background Art

[0002] Woody plant tissue culture involves inoculating explants—organs (such as stem segments, leaves, buds), tissues (such as cambium and phloem), or cells—onto a prepared culture medium under sterile conditions, allowing them to grow and differentiate under suitable culture conditions to form complete plants. A stem segment, a portion of a plant stem, is an important explant material in woody plant tissue culture. It contains structures such as nodes, internodes, and axillary buds. Nodes are the areas on the stem where leaves and buds are attached, while internodes are the areas between adjacent nodes. Axillary buds, located in the axils of leaves, have the potential to differentiate into new branches. Axillary and terminal buds on stem segments have a strong regenerative capacity. Under suitable culture medium and conditions, these buds can rapidly initiate growth and differentiate into adventitious buds and roots.

[0003] In the prior art, during the initiation of tissue culture of woody plants, when stem segments are used as explants, the leaves of the branches need to be cut off one by one with scissors after they are harvested, and then the branches need to be cut into 1.5cm-2.5cm stem segments according to the length of the internodes before they can enter subsequent processing.

[0004] However, the existing process of making stem segments is cumbersome, resulting in a time-consuming process, which affects the efficiency of stem segment production. When processing branches, the branches are exposed to the air for a long time, which also affects the quality of the start-up culture. Utility Model Content

[0005] The utility model aims to provide a device for preparing woody plant tissue culture stem segment explants, which solves the problem in the prior art that the process of making stem segments is complicated, resulting in a long working process and affecting the efficiency of stem segment making.

[0006] In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0007] A device for preparing woody plant tissue culture stem segment explants comprises a processing table and a conveyor belt located on the top of the processing table, wherein both sides of the conveyor belt are fixedly connected to support rods, the surfaces of the support rods are provided with blades through an adjustment mechanism, the surface of the conveyor belt is provided with a positioning mechanism, the top of the processing table is fixedly connected to a dividing table, the top of the dividing table is fixedly connected to an L-shaped cutting frame, and the surface of the cutting frame is provided with a plurality of cutting knives through an extrusion mechanism.

[0008] Preferably, the adjustment mechanism includes an adjustment rod passing through the surface of the support rod, and one end of the adjustment rod is fixedly connected to the blade.

[0009] Preferably, a plurality of anti-movement grooves are provided on the surface of the adjustment rod, and an L-shaped anti-movement rod is rotatably connected to the surface of the support rod, with one end of the anti-movement rod being located inside an adjacent anti-movement groove.

[0010] Preferably, the positioning mechanism includes a supporting block fixedly connected to the surface of the conveyor belt, the surface of the conveyor belt is fixedly connected to a clamping ring, the top of the clamping ring is threadedly connected to a screw, and the bottom of the screw is rotatably connected to the clamping block.

[0011] Preferably, the extrusion mechanism includes two movable rods passing through the top of the cutting frame, a lifting rod is fixedly connected between the two movable rods, the lifting rod is passed through the inside of several cutting knives, and the top of the lifting rod is slidably connected to a U-shaped auxiliary rod, and several insertion grooves are opened on the surface of the auxiliary rod, and the cutting knives are located inside adjacent insertion grooves.

[0012] Preferably, a compression spring is elastically connected between the lifting rod and the bottom of the cutting frame, a motor is fixedly connected to the top of the cutting frame, the output shaft of the motor is transmission-connected to two cams, and the top of the moving rod is arc-shaped.

[0013] The utility model has the following beneficial effects:

[0014] When making stem segments, the leaves on the branches can be quickly cleaned through the cooperation of the conveyor belt and the blade, thereby improving the efficiency of leaf removal. During the cleaning process, the stability of the branches is ensured by the clamping ring and the clamping block, which facilitates the removal of leaves from the branches. Later, when the branches need to be cut into stem segments, the spacing of the cutting knives can be adjusted, and then the cutting knife is moved downward to ensure the efficiency and accuracy of the cutting. The motor squeezes the moving rod through the cam to make the cutting knife drop, and then resets it upward through the elastic force of the compression spring. No manual reset is required, so that subsequent cutting can be carried out more conveniently, which greatly improves the efficiency of making branches into stem segments, reduces the time the branches are exposed to the air, and ensures the quality of the start-up culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0016] Figure 1 It is a structural diagram of the utility model;

[0017] Figure 2 for Figure 1 Schematic diagram of the side structure;

[0018] Figure 3 for Figure 1 Schematic diagram of the side structure of the middle dividing table;

[0019] Figure 4 for Figure 1 Schematic diagram of the structure of the conveyor belt;

[0020] Figure 5 for Figure 3 Schematic diagram of the side structure of the auxiliary rod and cutting knife.

[0021] In the figure: 1. Processing table; 2. Conveyor belt; 3. Support rod; 4. Adjustment mechanism; 5. Blade; 6. Positioning mechanism; 7. Cutting table; 8. Cutting frame; 9. Extrusion mechanism; 10. Cutting knife; 401. Adjustment rod; 402. Anti-movement groove; 403. Anti-movement rod; 601. Support block; 602. Clamping ring; 603. Screw; 604. Clamping block; 901. Moving rod; 902. Lifting rod; 903. Auxiliary rod; 904. Insertion groove; 905. Compression spring; 906. Motor; 907. Cam. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0023] Reference Figure 1-5 , a woody plant tissue culture stem segment explant preparation device, including a processing table 1 and a conveyor belt 2 located on the top of the processing table 1, the conveyor belt 2 is a conveyor belt 2 in the prior art, which is an existing mature technology, and both sides of the conveyor belt 2 are fixedly connected with support rods 3, and the surface of the support rods 3 is provided with blades 5 through an adjustment mechanism 4, and the surface of the conveyor belt 2 is provided with a positioning mechanism 6. When making stem segments, branches can be placed on the conveyor belt 2, and then the two ends of the branches are restricted by the positioning mechanism 6 on the conveyor belt 2, and then the conveyor belt 2 is started so that the positioning mechanism 6 drives the branches to move, thereby making the branches It passes through between the two supporting rods 3 so that the blade 5 can remove the leaves on the branches. In this process, it is necessary to first adjust the position of the blade 5 through the adjustment mechanism 4 to achieve a better effect of removing leaves. The top of the processing table 1 is fixedly connected to a cutting table 7, and the top of the cutting table 7 is fixedly connected to an L-shaped cutting frame 8. The surface of the cutting frame 8 is provided with a plurality of cutting knives 10 through an extrusion mechanism 9. After removing the leaves on the branches, the branches are placed on the cutting table 7, and then the extrusion mechanism 9 on the cutting frame 8 is started, so that the cutting knife 10 descends to cut the branches into several stem segments, which is convenient for subsequent use.

[0024] Furthermore, the adjustment mechanism 4 includes an adjustment rod 401 that passes through the surface of the support rod 3. One end of the adjustment rod 401 is fixedly connected to the blade 5. When the spacing between the blades 5 needs to be adjusted to facilitate the removal of leaves from branches of different thicknesses, the adjustment rod 401 can be moved to change the spacing between the two blades 5.

[0025] Furthermore, a plurality of anti-movement grooves 402 are provided on the surface of the adjustment rod 401, and an L-shaped anti-movement rod 403 is rotatably connected to the surface of the support rod 3. One end of the anti-movement rod 403 is located inside the adjacent anti-movement groove 402. After the position of the adjustment rod 401 is changed, the anti-movement rod 403 can be rotated so that one end of the anti-movement rod 403 enters the adjacent anti-movement groove 402. At this time, the adjustment rod 401 will be unable to move due to the obstruction of the anti-movement rod 403, thereby ensuring that the blade 5 will not be able to remove leaves.

[0026] Furthermore, the positioning mechanism 6 includes a supporting block 601 fixedly connected to the surface of the conveyor belt 2, and a clamping ring 602 is fixedly connected to the surface of the conveyor belt 2. The top of the clamping ring 602 is threadedly connected to a screw 603, and the bottom of the screw 603 is rotatably connected to the clamping block 604. When it is necessary to remove leaves from a branch, the branch is first placed on the conveyor belt 2, and then one end of the branch is pressed against the supporting block 601, and the other end of the branch is placed inside the clamping ring 602. Then the screw 603 is rotated, and the screw 603 is lowered to cause the clamping block 604 to descend synchronously. The clamping block 604 and the clamping ring 602 clamp and fix one end of the branch, so that when the transmission belt drives the clamping ring 602 to move, it can drive the branch to move. The branch starts to remove leaves from one end of the clamping ring 602 through the two blades 5, and ends when the branch at one end of the supporting block 601 leaves between the two blades 5, making it more convenient to fix the branch in a fixed position when removing leaves.

[0027] Furthermore, the squeezing mechanism 9 includes two moving rods 901 that pass through the top of the cutting frame 8, and a lifting rod 902 is fixedly connected between the two moving rods 901. The lifting rod 902 is passed through the interior of several cutting knives 10. The top of the lifting rod 902 is slidably connected to a U-shaped auxiliary rod 903. The surface of the auxiliary rod 903 is provided with several insertion grooves 904, and the cutting knives 10 are located inside adjacent insertion grooves 904. When cutting the branches with leaves removed, the branches are first placed on the processing table 1, and then the auxiliary rod 903 is lifted up to expose the cutting knives 10, and then the spacing between the several cutting knives 10 is adjusted so that the spacing is suitable for cutting the branches, and then the auxiliary rod 903 is lowered so that a part of the cutting knife 10 enters the position of the cutting knife 10 fixed inside the insertion groove 904, and then the lifting rod 902 is lowered by the moving rod 901, so that the cutting knife 10 cuts the branches.

[0028] Furthermore, a compression spring 905 is elastically connected between the lifting rod 902 and the bottom of the cutting frame 8, and a motor 906 is fixedly connected to the top of the cutting frame 8. The output shaft of the motor 906 is transmission-connected to two cams 907. The top of the moving rod 901 is arc-shaped. When it is necessary to lower the lifting rod 902, the motor 906 can be started, and the motor 906 drives the cam 907 to rotate. The cam 907 squeezes the tops of the two moving rods 901, causing the moving rod 901 to descend and drive the lifting rod 902 to descend, thereby enabling the cutting knife 10 to descend and cut the branches. After cutting, the cam 907 continues to rotate and no longer squeezes the moving rod 901. The lifting rod 902 can be reset upward by the elastic force of the compression spring 905. At this time, the motor 906 is turned off, the material that has become the stem segment is removed and replaced with a new branch to continue cutting. The arc-shaped top of the moving rod 901 can facilitate the cam 907 to apply force to the moving rod 901.

[0029] In summary:

[0030] When it is necessary to process the branch into a stem segment, the branch is first placed on the conveyor belt 2, and then one end of the branch is attached to the support block 601, and the other end of the branch passes through the clamping ring 602, and then the screw 603 is rotated to make the clamping block 604 at the bottom of the screw 603 drop to cooperate with the clamping ring 602 to clamp one end of the branch, and then the anti-movement rod 403 is rotated to leave the anti-movement groove 402, and then the position of the adjusting rod 401 is moved to adjust the spacing between the two blades 5. After adjustment, the anti-movement rod 403 is rotated again to re-enter the adjacent anti-movement groove 402 so that the blade 5 is fixed and can stably cut off the leaves, and then the conveyor belt 2 on the processing table 1 is started. The conveyor belt 2 drives the branch to move toward the blade 5 through the clamping ring 602 and the support block 601, so that the branch is removed by the two blades 5. The branch without leaves is then placed on the cutting table 7 , and then move the auxiliary rod 903 upward to release the cutting knife 10, adjust the spacing of the cutting knife 10 on the lifting rod 902, and lower the auxiliary rod 903 after adjustment so that the blade 5 enters the adjacent insertion slot 904, and then start the motor 906, the motor 906 squeezes the moving rod 901 through the cam 907, so that the moving rod 901 drives the lifting rod 902 to descend, so that the cutting knife 10 on the lifting rod 902 cuts the branch. After that, when the cam 907 no longer squeezes the moving rod 901, the lifting rod 902 can be reset upward by the elasticity of the compression spring 905, so that the cutting knife 10 is separated from the stem segment, so that the branch can be conveniently cut into stem segments for use. Through the above structure, it is convenient to remove the leaves on the branch by the blade 5 first, and then cut the branch into stem segments by the cutting knife 10, thereby improving the efficiency of branch processing and ensuring the quality of cultivation.

[0031] The above shows and describes the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions merely illustrate the principles of the present invention. Various changes and improvements are possible without departing from the spirit and scope of the present invention. Such changes and improvements are intended to fall within the scope of the present invention. The scope of protection claimed for the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for preparing woody plant tissue culture stem segment explants, comprising a processing table (1) and a conveyor belt (2) located on the top of the processing table (1), characterized in that: Both sides of the conveyor belt (2) are fixedly connected to support rods (3), and the surface of the support rods (3) is provided with blades (5) through an adjustment mechanism (4). The surface of the conveyor belt (2) is provided with a positioning mechanism (6). The top of the processing table (1) is fixedly connected to a cutting table (7), and the top of the cutting table (7) is fixedly connected to an L-shaped cutting frame (8), and the surface of the cutting frame (8) is provided with a plurality of cutting knives (10) through an extrusion mechanism (9).

2. The device for preparing woody plant tissue culture stem explants according to claim 1, wherein: The adjustment mechanism (4) comprises an adjustment rod (401) passing through the surface of the support rod (3), and one end of the adjustment rod (401) is fixedly connected to the blade (5).

3. The device for preparing woody plant tissue culture stem segment explants according to claim 2, wherein: The surface of the adjustment rod (401) is provided with a plurality of anti-movement grooves (402), and the surface of the support rod (3) is rotatably connected to an L-shaped anti-movement rod (403), one end of the anti-movement rod (403) is located inside an adjacent anti-movement groove (402).

4. The device for preparing woody plant tissue culture stem explants according to claim 1, wherein: The positioning mechanism (6) comprises a supporting block (601) fixedly connected to the surface of the conveyor belt (2); a clamping ring (602) is fixedly connected to the surface of the conveyor belt (2); a screw rod (603) is threadedly connected to the top of the clamping ring (602); and a clamping block (604) is rotatably connected to the bottom of the screw rod (603).

5. The device for preparing woody plant tissue culture stem segment explants according to claim 1, characterized in that: The squeezing mechanism (9) comprises two moving rods (901) passing through the top of the cutting frame (8); a lifting rod (902) is fixedly connected between the two moving rods (901); the lifting rod (902) passes through the interior of a plurality of cutting knives (10); a U-shaped auxiliary rod (903) is slidably connected to the top of the lifting rod (902); a plurality of insertion slots (904) are provided on the surface of the auxiliary rod (903); and the cutting knives (10) are located inside adjacent insertion slots (904).

6. The device for preparing woody plant tissue culture stem segment explants according to claim 5, characterized in that: A compression spring (905) is elastically connected between the lifting rod (902) and the bottom of the cutting frame (8); a motor (906) is fixedly connected to the top of the cutting frame (8); an output shaft of the motor (906) is transmission-connected to two cams (907); and the top of the moving rod (901) is in an arc shape.

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