Quantitative extraction system for nucleic acid extraction
Through the innovative design of the sample tube and puncture needle assembly, low-cost and efficient sample transfer of the nucleic acid extraction system is achieved, which solves the high cost and complex operation problems of the existing system and improves the ease of operation and space utilization.
Patent Information
- Application Number
- CN202422707231.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-06
- Publication Date
- 2025-09-19
- Estimated Expiration
- 2034-11-06
AI Technical Summary
Existing nucleic acid extraction systems are expensive, require pipettes and consumables, are complex to operate, and take up a large amount of space.
Using components such as sample tubes, pressurized puncture needles, puncture needles and purification tubes, sample transfer is achieved through gas pressure and liquid channels, avoiding the use of pipettes and simplifying the operation process.
It reduces costs, simplifies operations, improves efficiency, reduces consumables, and reduces size.
Smart Images
Figure CN223357634U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to a quantitative extraction system for nucleic acid extraction. Background Art
[0002] Currently, nucleic acids are often extracted using quantitative extraction systems. Existing quantitative extraction systems for nucleic acid extraction generally include a sample tube, a first quantitative tube, and a purification tube. During use, the top cover of the sample tube must first be opened, and the sample must be removed from the sample tube using a pipette. The sample is then transferred to the purification tube for reaction purification. During this process, the pipette is required to aspirate and transfer the sample. However, due to the high cost of pipettes and the large number of consumables such as pipette tips, the cost is high and far from meeting industry requirements. Subsequently, the purified sample can be transferred to a PCR tube as needed. Utility Model Content
[0003] In order to overcome the deficiencies of the prior art, the purpose of the present invention is to provide a quantitative extraction system for nucleic acid extraction, which can reduce costs.
[0004] The purpose of this utility model is achieved by the following technical solutions:
[0005] A quantitative extraction system for nucleic acid extraction comprises a sample tube, a pressurized puncture needle, a first puncture needle, a first quantitative tube, a second puncture needle, a purification tube, and a third puncture needle; the pressurized puncture needle is used to puncture the top of the sample tube, and the pressurized puncture needle is provided with a ventilation channel for introducing gas into the sample tube; the first puncture needle is used to puncture the bottom of the sample tube, and the first puncture needle is provided with a first liquid passage for allowing liquid to flow into the first quantitative tube; the second puncture needle is used to puncture the bottom of the first quantitative tube, and the second puncture needle is provided with a second liquid passage for allowing liquid to flow into the purification tube; the third puncture needle is used to puncture the bottom of the purification tube, and the third puncture needle is provided with a third liquid passage for allowing liquid to flow through.
[0006] The quantitative extraction system for nucleic acid extraction also includes a second quantitative tube and a fourth puncture needle; the second quantitative tube is used to receive liquid flowing out of the third liquid channel; the fourth puncture needle is used to pierce the bottom of the second quantitative tube; the fourth puncture needle is provided with a fourth liquid channel for liquid to flow through.
[0007] The first quantitative tube and the second quantitative tube both include a first main body and a quantitative bottom plug; a leakage bin is provided in the first main body, the first main body is provided with a built-in wall extending into the leakage bin, an upper quantitative bin is formed in the built-in wall, the quantitative bottom plug is provided on the first main body, the quantitative bottom plug is provided with a lower quantitative bin, and the lower quantitative bin is connected to the upper quantitative bin; at least a portion of the cavity wall of the lower quantitative bin of the first quantitative tube is formed as a first matching wall suitable for puncturing by the second puncture needle; at least a portion of the cavity wall of the lower quantitative bin of the second quantitative tube is formed as a second matching wall suitable for puncturing by the fourth puncture needle.
[0008] The quantitative bottom plug includes a bottom surrounding wall and a partition wall; the partition wall is arranged inside the bottom surrounding wall, and the bottom surrounding wall and the partition wall form the lower quantitative bin; the partition wall of the quantitative bottom plug of the first quantitative tube is formed as the first matching wall; the partition wall of the quantitative bottom plug of the second quantitative tube is formed as the second matching wall.
[0009] The top surface of the built-in wall is lower than the top surface of the first main pipe.
[0010] The built-in wall includes a transverse wall body and a built-in tube wall; the transverse wall body is arranged on the first main tube body, and the built-in tube wall is arranged on the transverse wall body and extends upward; the upper quantitative bin extends from the inside of the built-in tube wall to the transverse wall body.
[0011] The top surface of the built-in tube wall is lower than the top surface of the first main tube.
[0012] The sample tube includes a second main body, an upper rubber plug and a lower rubber plug; the upper rubber plug is arranged on the upper end of the second main body, and the lower rubber plug is arranged on the lower end of the second main body; the pressurized puncture needle is used to pierce the upper rubber plug, and the first puncture needle is used to pierce the lower rubber plug.
[0013] The purification tube comprises a third main body and a purification rubber plug; the purification rubber plug is arranged on the lower end of the third main body; and the third puncture needle is used to puncture the purification rubber plug.
[0014] The third main body and the purification rubber plug form a reaction chamber, and the third main body is provided with a waste liquid hole communicated with the reaction chamber.
[0015] Compared with the prior art, the beneficial effects of the present invention are:
[0016] The utility model provides a quantitative extraction system for nucleic acid extraction, which adopts a combination of a sample tube, a pressurized puncture needle, a first puncture needle, a first quantitative tube, a second puncture needle, a purification tube, and a third puncture needle. It can realize the transfer of samples from the sample tube via the first quantitative tube to the purification tube without the need for a pipette, which can reduce costs, make the operation simpler, save time, and improve efficiency. In addition, it can also reduce the volume, reduce the required occupied space, and reduce consumables. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 It is a structural diagram of the utility model;
[0018] Figure 2 It is a cross-sectional view of the utility model;
[0019] Figure 3 Schematic diagram of the structure of the second quantitative tube;
[0020] Figure 4 This is an exploded view of the first quantitative tube;
[0021] Figure 5 is a cross-sectional view of the first quantitative tube;
[0022] Among them, 10, quantitative bottom plug; 11, lower quantitative chamber; 12, first matching wall; 13, bottom surrounding wall; 15, bottom cavity; 16, outer protrusion; 20, first main body; 21, leakage chamber; 30, built-in wall; 31, horizontal wall; 32, upper quantitative chamber; 33, main chamber section; 34, guide section; 35, built-in tube wall; 40, sample tube; 41, second main body; 42, upper rubber plug; 43, lower rubber plug; 50 , pressurized puncture needle; 51, ventilation channel; 52, first puncture needle; 53, first liquid channel; 54, second puncture needle; 55, second liquid channel; 57, third puncture needle; 58, third liquid channel; 60, first quantitative tube; 61, second quantitative tube; 70, purification tube; 71, third main body; 72, purification plug; 73, reaction chamber; 74, waste liquid hole; 81, fourth puncture needle; 82, fourth liquid channel. DETAILED DESCRIPTION
[0023] Below, the present invention is further described in conjunction with the accompanying drawings and specific implementation methods. It should be noted that, under the premise of no conflict, the various embodiments or technical features described below can be arbitrarily combined to form new embodiments.
[0024] like Figure 1-5As shown, a quantitative extraction system for nucleic acid extraction includes a sample tube 40, a pressurized puncture needle 50, a first puncture needle 52, a first quantitative tube 60, a second puncture needle 54, a purification tube 70, and a third puncture needle 57; the pressurized puncture needle 50 is used to puncture the top of the sample tube 40, and the pressurized puncture needle 50 is provided with a ventilation channel 51 for introducing gas into the sample tube 40; the first puncture needle 52 is used to puncture the bottom of the sample tube 40, and the first puncture needle 52 is provided with a first liquid passage 53 for allowing liquid to flow into the first quantitative tube 60; the second puncture needle 54 is used to puncture the bottom of the first quantitative tube 60, and the second puncture needle 54 is provided with a second liquid passage 55 for allowing liquid to flow into the purification tube 70; the third puncture needle 57 is used to puncture the bottom of the purification tube 70, and the third puncture needle 57 is provided with a third liquid passage 58 for allowing liquid to flow through.
[0025] During use, the pressurized puncture needle 50 pierces the top of the sample tube 40, the first puncture needle 52 pierces the bottom of the sample tube 40, and then the high-pressure gas is introduced into the sample tube 40 through the ventilation channel 51 of the pressurized puncture needle 50, so that the sample in the sample tube 40 can flow into the first quantitative tube 60 through the first liquid passage 53 of the first puncture needle 52, and then the second puncture needle 54 pierces the bottom of the first quantitative tube 60, and the quantitative sample in the first quantitative tube 60 flows into the purification tube 70 through the second liquid passage 55 of the second puncture needle 54, and then the third puncture needle 57 pierces the bottom of the purification tube 70, and the pure The purified sample in the purification tube 70 flows out through the third liquid passage 58 of the third puncture needle 57. Therefore, the present invention provides a quantitative extraction system for nucleic acid extraction, which adopts a combination of the sample tube 40, the pressurized puncture needle 50, the first puncture needle 52, the first quantitative tube 60, the second puncture needle 54, the purification tube 70, and the third puncture needle 57, so that the sample can be transferred from the sample tube 40 to the purification tube 70 via the first quantitative tube 60 without using a pipette, which can reduce costs, and the operation is relatively simple, which can save time and improve efficiency; moreover, it can also reduce the volume and reduce the required space.
[0026] The quantitative extraction system for nucleic acid extraction also includes a second quantitative tube 61 and a fourth puncture needle 81. The second quantitative tube 61 is used to receive liquid flowing out of the third liquid passage 58. The fourth puncture needle 81 is used to pierce the bottom of the second quantitative tube 61. The fourth puncture needle 81 is provided with a fourth liquid passage 82 for liquid to flow through. In practice, the purified sample in the purification tube 70 flows into the second quantitative tube 61 through the third liquid passage 58 of the third puncture needle 57, and the quantitative sample in the second quantitative tube 61 can flow out through the fourth liquid passage 82 of the fourth puncture needle 81.
[0027] The first quantitative tube 60 and the second quantitative tube 61 both include a first main body 20 and a quantitative bottom plug 10; a leakage bin 21 is provided in the first main body 20, and the first main body 20 is provided with a built-in wall 30 extending into the leakage bin 21, and an upper quantitative bin 32 is formed in the built-in wall 30, and the quantitative bottom plug 10 is provided on the first main body 20, and the quantitative bottom plug 10 is provided with a lower quantitative bin 11, and the lower quantitative bin 11 is connected to the upper quantitative bin 32; at least a portion of the cavity wall of the lower quantitative bin 11 of the first quantitative tube 60 is formed as a first matching wall 12 suitable for puncturing by the second puncture needle 54; at least a portion of the cavity wall of the lower quantitative bin 11 of the second quantitative tube 61 is formed as a second matching wall suitable for puncturing by the fourth puncture needle 81. During use, liquid flows into the upper quantitative chamber 32 and the lower quantitative chamber 11. When the liquid fills the upper quantitative chamber 32 and the lower quantitative chamber 11, the excess liquid overflows into the leakage chamber 21, thereby maintaining a constant liquid volume in the upper quantitative chamber 32 and the lower quantitative chamber 11 of the first quantitative tube 60 and the upper quantitative chamber 32 and the lower quantitative chamber 11 of the second quantitative tube 61. The second puncture needle 54 pierces the first matching wall 12, allowing the liquid in the upper quantitative chamber 32 and the lower quantitative chamber 11 to flow to the purification tube 70 through the second liquid passage 55 of the second puncture needle 54. The fourth puncture needle 81 pierces the second matching wall, allowing the liquid in the upper quantitative chamber 32 and the lower quantitative chamber 11 of the second quantitative tube 61 to flow out through the fourth liquid passage 82 of the fourth puncture needle 81.
[0028] The quantitative bottom plug 10 includes a bottom surrounding wall 13 and a partition wall. The partition wall is disposed within the bottom surrounding wall 13, and the bottom surrounding wall 13 and the partition wall enclose the lower quantitative chamber 11. The partition wall of the quantitative bottom plug 10 of the first quantitative tube 60 forms the first mating wall 12, and the partition wall of the quantitative bottom plug 10 of the second quantitative tube 61 forms the second mating wall. This arrangement facilitates the formation of the lower quantitative chamber 11 while reducing costs.
[0029] The dosing bottom plug 10 is further provided with a bottom cavity 15. The lower dosing chamber 11 is separated from the bottom cavity 15 by a partition wall. The lower dosing chamber 11 is located above the partition wall, and the bottom cavity 15 is located below the partition wall. This arrangement also reduces the material used for the dosing bottom plug 10, thereby lowering costs.
[0030] The lower end of the bottom surrounding wall 13 is further provided with an outwardly protruding outer body 16, and the outer body 16 is supported below the first main body 20. By adopting the above arrangement, the stability of the cooperation between the quantitative bottom plug 10 and the first main body 20 can be improved.
[0031] At least a portion of the bottom surrounding wall 13 is embedded in the first main body 20. By adopting the above arrangement, the stability of the cooperation between the quantitative bottom plug 10 and the first main body 20 can be further improved.
[0032] The top surface of the built-in wall 30 is lower than the top surface of the first main body 20. In use, when the liquid fills the upper quantitative chamber 32 and the lower quantitative chamber 11, the excess liquid can flow over the top surface of the built-in wall 30 into the leakage chamber 21.
[0033] The internal wall 30 includes a transverse wall 31 and an internal tubular wall 35. The transverse wall 31 is disposed on the first main body 20, and the internal tubular wall 35 is disposed on the transverse wall 31 and extends upward. The upper metering chamber 32 extends from the interior of the internal tubular wall 35 to the transverse wall 31. The top surface of the internal tubular wall 35 is lower than the top surface of the first main body 20. This arrangement facilitates the manufacture and formation of the upper metering chamber 32 and reduces the manufacturing cost of the internal wall 30.
[0034] Preferably, the upper quantitative chamber 32 includes a main chamber section 33 and a guide section 34; the guide section 34 is located below the main chamber section 33 and is in communication with the main chamber section 33. The diameter of the guide section 34 gradually decreases from the upper end to the lower end of the guide section 34. By adopting this structure, the liquid in the upper quantitative chamber 32 can be conveniently guided into the lower quantitative chamber 11 through the guide section 34.
[0035] Specifically, the main compartment section 33 is formed in the built-in tube wall 35, and the guide section 34 is formed on the transverse wall 31. By adopting the above arrangement, processing can be facilitated.
[0036] The sample tube 40 includes a second main body 41, an upper rubber plug 42, and a lower rubber plug 43. The upper rubber plug 42 is disposed on the upper end of the second main body 41, and the lower rubber plug 43 is disposed on the lower end of the second main body 41. The pressurized puncture needle 50 is used to pierce the upper rubber plug 42, and the first puncture needle 52 is used to pierce the lower rubber plug 43. This arrangement facilitates simultaneous puncture by the pressurized puncture needle 50 and the first puncture needle 52, and facilitates the manufacture of the sample tube 40.
[0037] The purification tube 70 includes a third main body 71 and a purification rubber plug 72. The purification rubber plug 72 is disposed at the lower end of the third main body 71. The third puncture needle 57 is used to puncture the purification rubber plug 72. This arrangement facilitates puncture by the third puncture needle 57 and facilitates the processing and manufacture of the purification tube 70.
[0038] The third main body 71 and the purification plug 72 form a reaction chamber 73. The third main body 71 is provided with a waste liquid hole 74 connected to the reaction chamber 73. The sample flowing into the reaction chamber 73 through the second liquid passage 55 of the second puncture needle 54 reacts with the added reagent in the reaction chamber 73 for purification. The waste liquid hole 74 can be used for mixing by suction and exhalation, and waste liquid can be extracted, which is convenient for operation.
[0039] The above-mentioned embodiments are only preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantial changes and replacements made by technicians in this field on the basis of the present invention fall within the scope of protection required by the present invention.
Claims
1. A quantitative extraction system for nucleic acid extraction, characterized in that: It includes a sample tube, a pressurized puncture needle, a first puncture needle, a first quantitative tube, a second puncture needle, a purification tube, and a third puncture needle; the pressurized puncture needle is used to puncture the top of the sample tube, and the pressurized puncture needle is provided with a ventilation channel for introducing gas into the sample tube; the first puncture needle is used to puncture the bottom of the sample tube, and the first puncture needle is provided with a first liquid passage for allowing liquid to flow into the first quantitative tube; the second puncture needle is used to puncture the bottom of the first quantitative tube, and the second puncture needle is provided with a second liquid passage for allowing liquid to flow into the purification tube; the third puncture needle is used to puncture the bottom of the purification tube, and the third puncture needle is provided with a third liquid passage for allowing liquid to flow through.
2. The quantitative extraction system for nucleic acid extraction according to claim 1, wherein: The quantitative extraction system for nucleic acid extraction also includes a second quantitative tube and a fourth puncture needle; the second quantitative tube is used to receive liquid flowing out of the third liquid channel; the fourth puncture needle is used to pierce the bottom of the second quantitative tube; the fourth puncture needle is provided with a fourth liquid channel for liquid to flow through.
3. The quantitative extraction system for nucleic acid extraction according to claim 2, wherein: The first quantitative tube and the second quantitative tube both include a first main body and a quantitative bottom plug; a leakage bin is provided in the first main body, the first main body is provided with a built-in wall extending into the leakage bin, an upper quantitative bin is formed in the built-in wall, the quantitative bottom plug is provided on the first main body, the quantitative bottom plug is provided with a lower quantitative bin, and the lower quantitative bin is connected to the upper quantitative bin; at least a portion of the cavity wall of the lower quantitative bin of the first quantitative tube is formed as a first matching wall suitable for puncturing by the second puncture needle; at least a portion of the cavity wall of the lower quantitative bin of the second quantitative tube is formed as a second matching wall suitable for puncturing by the fourth puncture needle.
4. The quantitative extraction system for nucleic acid extraction according to claim 3, wherein: The quantitative bottom plug includes a bottom surrounding wall and a partition wall; the partition wall is arranged inside the bottom surrounding wall, and the bottom surrounding wall and the partition wall form the lower quantitative bin; the partition wall of the quantitative bottom plug of the first quantitative tube is formed as the first matching wall; the partition wall of the quantitative bottom plug of the second quantitative tube is formed as the second matching wall.
5. The quantitative extraction system for nucleic acid extraction according to claim 3, wherein: The top surface of the built-in wall is lower than the top surface of the first main pipe.
6. The quantitative extraction system for nucleic acid extraction according to claim 5, characterized in that: The built-in wall includes a transverse wall body and a built-in tube wall; the transverse wall body is arranged on the first main tube body, and the built-in tube wall is arranged on the transverse wall body and extends upward; the upper quantitative bin extends from the inside of the built-in tube wall to the transverse wall body.
7. The quantitative extraction system for nucleic acid extraction according to claim 6, characterized in that: The top surface of the built-in tube wall is lower than the top surface of the first main tube.
8. The quantitative extraction system for nucleic acid extraction according to claim 1, wherein: The sample tube includes a second main body, an upper rubber plug and a lower rubber plug; the upper rubber plug is arranged on the upper end of the second main body, and the lower rubber plug is arranged on the lower end of the second main body; the pressurized puncture needle is used to pierce the upper rubber plug, and the first puncture needle is used to pierce the lower rubber plug.
9. The quantitative extraction system for nucleic acid extraction according to claim 1, wherein: The purification tube comprises a third main body and a purification rubber plug; the purification rubber plug is arranged on the lower end of the third main body; and the third puncture needle is used to puncture the purification rubber plug.
10. The quantitative extraction system for nucleic acid extraction according to claim 9, characterized in that: The third main body and the purification rubber plug form a reaction chamber, and the third main body is provided with a waste liquid hole communicated with the reaction chamber.