Enzyme production system

By designing a parallel enzyme production system, the problems of high equipment investment, complex processes and difficulty in improving product quality in enzyme production were solved, and efficient production and quality improvement were achieved.

CN223373074UActive Publication Date: 2025-09-23SHANDONG JUHONG BIOTECHNOLOGY RES INST CO LTD
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Patent Information

Application Number
CN202422582390.X
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-10-25
Publication Date
2025-09-23
Estimated Expiration
2034-10-25

AI Technical Summary

Technical Problem

The existing enzyme production process has problems such as difficulty in ensuring fermentation environmental hygiene, high equipment investment and operating costs, complex process flow and poor equipment correlation, resulting in the inability to improve product quality.

Method used

An enzyme production system was designed, including extraction tanks, mixing tanks, seed tanks, fermentation tanks and enzymolysis tanks, which were connected in parallel to form multiple complete production lines. The use of the tanks was controlled by control valves, and combined with heating, cooling and cleaning systems to achieve flexible process flow combinations.

Benefits of technology

It improves the efficiency of enzyme production and product quality, meets the needs of different reactions, reduces equipment investment and operating costs, and forms a complete production line.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model provides an enzyme production system, which relates to the technical field of reaction tanks, and comprises an extraction tank, a stirring tank, a seed tank, a plurality of fermentation tanks and a plurality of enzymolysis tanks, the material outlet of the extraction tank is connected with the material inlet of the stirring tank, the material outlet of the seed tank is connected with the material inlet of the stirring tank, and the material outlet of the enzymolysis tank is connected with the material inlet of the stirring tank. The material inlet of the fermentation tank is respectively connected with the material outlets of the extraction tank, the stirring tank and the seed tank, the material inlet of the enzymolysis tank is respectively connected with the material outlets of the stirring tank and the seed tank, and the material outlet of the enzymolysis tank is connected with the material inlet of the fermentation tank; a material inlet of the heating tank is connected with a material outlet of the fermentation tank or the enzymolysis tank, and a material outlet of the buffer tank is connected with a material inlet of the extraction tank. And flexible combination can be realized in the enzyme production process to form a plurality of complete production lines, so that the production efficiency is improved, and the product quality is improved.
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Description

Technical Field

[0001] The utility model relates to the technical field of reaction tanks, in particular to an enzyme production system. Background Art

[0002] Enzyme production is the process of producing products containing specific bioactive ingredients (including polysaccharides, oligosaccharides, proteins and peptides, amino acids, and vitamins) from animal, plant, and fungal sources, with or without the addition of auxiliary materials, through microbial fermentation. This process utilizes various reactors to provide reaction conditions. These reactors not only ensure thorough mixing of the raw materials, accelerating the reaction, but also provide the required temperature and other reaction conditions.

[0003] The existing enzyme production process is still mainly based on homemade enzyme production, which is mainly due to the lack of guaranteed fermentation environment hygiene and the high risk of mold exceeding the standard. As people's quality of life improves, some companies have begun to produce enzymes. However, due to the large amount of equipment required and the correspondingly complex process flow and operation, the investment and operating costs of the equipment are high. Moreover, due to the poor correlation between most of the equipment, it is impossible to form a complete production line, resulting in the lack of improvement in product quality. Summary of the Invention

[0004] In response to the above problems of the existing technology, the utility model provides an enzyme production system, including an extraction tank, a stirring tank, a seed tank, a fermentation tank, an enzymolysis tank, a heating tank, a filter press and a buffer tank, etc., which can be flexibly combined in the enzyme production process to form multiple complete production lines, which is beneficial to improving production efficiency and improving product quality.

[0005] To this end, the technical solution of the present invention is an enzyme production system, which includes an extraction tank, a stirring tank, a seed tank, a fermentation tank and an enzymolysis tank. There are multiple fermentation tanks and multiple enzymolysis tanks. The material outlet of the extraction tank is connected to the material inlet of the stirring tank, the material outlet of the seed tank is connected to the material inlet of the stirring tank, the material inlet of the fermentation tank is respectively connected to the material outlets of the extraction tank, the stirring tank and the seed tank, the material inlet of the enzymolysis tank is respectively connected to the material outlets of the stirring tank and the seed tank, and the material outlet of the enzymolysis tank is connected to the material inlet of the fermentation tank.

[0006] Furthermore, the fermentation tank includes a reaction inner tube and a jacket, a stirring paddle is provided inside the reaction inner tube, an air duct is provided below the stirring paddle, a material inlet and a cleaning liquid inlet are provided at the upper end of the reaction inner tube, a material outlet, an air inlet and a sewage outlet are provided at the lower end of the reaction inner tube, a hot and cold water outlet is provided at the upper end of the jacket, and a hot and cold water inlet is provided at the lower end of the jacket.

[0007] Furthermore, a plurality of fermentation tanks are connected in parallel, and a control valve is provided on the material inlet and outlet pipelines of each fermentation tank.

[0008] Furthermore, a plurality of enzymolysis tanks are connected in parallel, and a control valve is provided on the material inlet and outlet pipelines of each enzymolysis tank.

[0009] Furthermore, an enzyme production system also includes a heating tank, a filter press and a buffer tank. The heating tank is provided with a reaction inner cylinder and a jacket. The upper end of the reaction inner cylinder is provided with a material inlet and a cleaning liquid inlet, the lower end of the reaction inner cylinder is provided with a material outlet and a sewage outlet, the upper end of the jacket is provided with a steam inlet, the lower end of the jacket is provided with a steam outlet, the upper end of the buffer tank is provided with a material inlet and a cleaning liquid inlet, and the lower end of the buffer tank is provided with a material outlet and a sewage outlet.

[0010] Furthermore, the material inlet of the heating tank is connected to the material outlet of the fermentation tank or the enzymolysis tank respectively, the material outlet of the heating tank is connected to the material inlet of the filter press, the material outlet of the filter press is connected to the material inlet of the buffer tank, and the material outlet of the buffer tank is connected to the material inlet of the extraction tank.

[0011] Furthermore, an enzyme production system also includes an alkali tank, a water tank and an acid tank, which are connected in parallel, and control valves are provided on the input and output pipes of the alkali tank, the water tank and the acid tank.

[0012] Furthermore, the liquid outlet of the alkali tank or the acid tank is respectively connected to the cleaning liquid inlet of the extraction tank, stirring tank, seed tank, fermentation tank and enzymolysis tank, and the liquid return port of the alkali tank or the acid tank is respectively connected to the sewage outlet of the extraction tank, stirring tank, seed tank, fermentation tank and enzymolysis tank.

[0013] Furthermore, an enzyme production system also includes a hot water tank and a cold water tank, the liquid outlets of the hot water tank and the cold water tank are respectively connected to the hot and cold water inlets of the seed tank, the fermentation tank and the enzymolysis tank, and the liquid return ports of the hot water tank and the cold water tank are respectively connected to the hot and cold water outlets of the seed tank, the fermentation tank and the enzymolysis tank.

[0014] The beneficial effect of the utility model is that purified water and materials are added to the extraction tank for extraction reaction. After the extraction is completed, the extract directly enters the fermentation tank, or the extract enters the stirring tank under the drive of a screw pump, or the seed liquid in the seed tank enters the stirring tank under the drive of a screw pump. The stirring tank performs operations such as stirring, mixing, blending, and homogenizing on the materials. Various processing methods can be flexibly selected to meet different reaction requirements; multiple fermentation tanks or multiple enzymolysis tanks are connected in parallel, and one or more of them can be used to meet the process requirements of different fermentation conditions; in the enzyme production process, they can be flexibly combined to form multiple complete production lines, which is beneficial to improving production efficiency and improving product quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0016] Figure 2 It is a schematic diagram of the upper structure of the utility model;

[0017] Figure 3 It is a schematic diagram of the middle structure of the utility model;

[0018] Figure 4 It is a schematic structural diagram of the lower part of the utility model.

[0019] Explanation of symbols in the figure:

[0020] 1. Extraction tank; 2. Mixing tank; 3. Seed tank; 4. Fermentation tank; 5. Enzyme hydrolysis tank; 6. Heating tank; 7. Filter press; 8. Buffer tank; 9. Alkali tank; 10. Water tank; 11. Acid tank; 12. Hot water tank; 13. Cold water tank. DETAILED DESCRIPTION

[0021] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0022] like Figures 1-4 As shown, the utility model provides an enzyme production system, which includes an extraction tank 1, a stirring tank 2, a seed tank 3, a fermentation tank 4 and an enzymolysis tank 5. The upper end of the extraction tank 1 is provided with a material inlet and a cleaning liquid inlet, the lower end of the extraction tank 1 is provided with a material outlet and a sewage outlet, the upper end of the stirring tank 2 is provided with a material inlet and a cleaning liquid inlet, the lower end of the stirring tank 2 is provided with a material outlet and a sewage outlet, the upper end of the seed tank 3 is provided with a material inlet and a cleaning liquid inlet, and the lower end of the seed tank 3 is provided with a material outlet and a sewage outlet.

[0023] The material outlet of the extraction tank 1 is connected to the material inlet of the stirring tank 2, a screw pump is provided between the material outlet of the extraction tank 1 and the material inlet of the stirring tank 2, the material outlet of the seed tank 3 is connected to the material inlet of the stirring tank 2, a screw pump is provided between the material outlet of the seed tank 3 and the material inlet of the stirring tank 2, purified water and material are added to the extraction tank 1 for extraction reaction, after the extraction is completed, the extract in the extraction tank 1 enters the stirring tank 2 under the drive of the screw pump, and the seed liquid in the seed tank 3 enters the stirring tank 2 under the drive of the screw pump, and the stirring tank 2 performs operations such as stirring, mixing, blending, and homogenizing on the material.

[0024] There are multiple fermentation tanks 4, and the multiple fermentation tanks 4 are connected in parallel. A control valve is provided on the material inlet and outlet pipelines of each fermentation tank 4. The control valve controls whether each fermentation tank 4 is used. One or more of the multiple fermentation tanks 4 can be used to meet the process requirements of different fermentation conditions. When multiple fermentation tanks 4 are used at the same time, the multiple fermentation tanks 4 are used in parallel.

[0025] The fermentation tank 4 includes a reaction inner tube and a jacket. A stirring paddle is provided inside the reaction inner tube, and an air duct is provided below the stirring paddle. The air duct is used to introduce compressed air to meet the oxygen demand of the fermentation tank 4. The upper end of the reaction inner tube is provided with a material inlet and a cleaning liquid inlet, and the lower end of the reaction inner tube is provided with a material outlet, an air inlet and a sewage outlet. The upper end of the jacket is provided with a hot and cold water outlet, and the lower end of the jacket is provided with a hot and cold water inlet.

[0026] The material inlet of the fermentation tank 4 is respectively connected to the material outlets of the extraction tank 1, the stirring tank 2, and the seed tank 3. The materials in the extraction tank 1, the stirring tank 2 or the seed tank 3 can be added to the fermentation tank 4. The material in the seed tank 3 can first be stirred and mixed with other basic culture media in the stirring tank 2 and then added to the fermentation tank 4. Different process flows can be flexibly set.

[0027] There are multiple enzymolysis tanks 5, and the multiple enzymolysis tanks 5 are connected in parallel. A control valve is provided on the material inlet and outlet pipelines of each enzymolysis tank 5. The control valve controls whether each enzymolysis tank 5 is used. One or more of the multiple enzymolysis tanks 5 can be used to meet the process flow requirements of different enzymolysis conditions. When multiple enzymolysis tanks 5 are used at the same time, the multiple enzymolysis tanks 5 are used in parallel.

[0028] The enzymatic hydrolysis tank 5 includes a reaction inner tube and a jacket. A stirring paddle is provided inside the reaction inner tube. The upper end of the reaction inner tube is provided with a material inlet and a cleaning liquid inlet. The lower end of the reaction inner tube is provided with a material outlet and a sewage outlet. The upper end of the jacket is provided with a hot and cold water outlet, and the lower end of the jacket is provided with a hot and cold water inlet.

[0029] The material inlet of the enzymolysis tank 5 is respectively connected to the material outlet of the stirring tank 2 and the material outlet of the seed tank 3. The material in the stirring tank 2 or the seed tank 3 can be added to the enzymolysis tank 5. The material in the seed tank 3 can first be stirred and mixed with other basic culture media in the stirring tank 2 and then added to the enzymolysis tank 5. Different process flows can be flexibly set.

[0030] The material outlet of the enzymolysis tank 5 is connected to the material inlet of the fermentation tank 4. After the enzymolysis liquid in the enzymolysis tank 5 is transferred to the fermentation tank 4, basic culture medium such as yeast powder and glucose is added. After sterilization is completed, the seed liquid in the seed tank 3 is transferred to the fermentation tank 4, the temperature is controlled at 30°C, and anaerobic fermentation is carried out.

[0031] An enzyme production system also includes a heating tank 6, a filter press 7 and a buffer tank 8. The heating tank 6 is provided with a reaction inner cylinder and a jacket. The upper end of the reaction inner cylinder is provided with a material inlet and a cleaning liquid inlet, the lower end of the reaction inner cylinder is provided with a material outlet and a sewage outlet, the upper end of the jacket is provided with a steam inlet, the lower end of the jacket is provided with a steam outlet, the upper end of the buffer tank 8 is provided with a material inlet and a cleaning liquid inlet, and the lower end of the buffer tank 8 is provided with a material outlet and a sewage outlet.

[0032] The material inlet of the heating tank 6 is connected to the material outlet of the fermentation tank 4 or the enzymolysis tank 5, respectively. The material outlet of the heating tank 6 is connected to the material inlet of the filter press 7, the material outlet of the filter press 7 is connected to the material inlet of the buffer tank 8, and the material outlet of the buffer tank 8 is connected to the material inlet of the extraction tank 1. After the material is processed by the heating tank 6, the filter press 7 and the buffer tank 8, the filtrate can be directly output or added to the extraction tank 1 as raw material.

[0033] An enzyme production system also includes an alkali tank 9, a water tank 10 and an acid tank 11, wherein the alkali tank 9, the water tank 10 and the acid tank 11 are connected in parallel, and the input and output pipelines of the alkali tank 9, the water tank 10 and the acid tank 11 are each provided with a separate control valve. According to the cleaning requirements of the above reaction tank, one of alkali liquor, acid solution or hot water is selected for cleaning. When the water tank 10 is used for cleaning, hot water can be recycled. When the alkali liquor or acid solution in the alkali tank 9 or the acid tank 11 is used for cleaning, the alkali liquor or acid solution needs to be recovered. The liquid outlet of the alkali tank 9 or the acid tank 11 is respectively connected to the cleaning liquid inlet of the extraction tank 1, the stirring tank 2, the seed tank 3, the fermentation tank 4 and the enzymolysis tank 5, and the liquid return port of the alkali tank 9 or the acid tank 11 is respectively connected to the sewage outlet of the extraction tank 1, the stirring tank 2, the seed tank 3, the fermentation tank 4 and the enzymolysis tank 5.

[0034] An enzyme production system also includes a hot water tank 12 and a cold water tank 13. The hot water tank 12 is used to heat other reaction tanks, while the cold water tank 13 is used to cool other reaction tanks. The hot water tank 12 and the cold water tank 13 are used together to control the reaction temperature of other reaction tanks. The liquid outlets of the hot water tank 12 and the cold water tank 13 are respectively connected to the hot and cold water inlets of the seed tank 3, the fermentation tank 4, and the enzymolysis tank 5. The liquid return ports of the hot water tank 12 and the cold water tank 13 are respectively connected to the hot and cold water outlets of the seed tank 3, the fermentation tank 4, and the enzymolysis tank 5.

[0035] The enzyme production system of the present invention can be flexibly combined during the enzyme production process to form multiple complete production lines, which is beneficial for improving production efficiency and product quality. For example, an enzyme production system, in actual production, comprises an extraction tank 1, a seed tank 3, a fermentation tank 4, an enzymolysis tank 5, a heating tank 6, a filter press 7, a buffer tank 8, a hot water tank 12, and a cold water tank 13. The specific process flow is as follows:

[0036] 1. Raw material pretreatment:

[0037] The raw materials are screened, cleaned to remove impurities and unqualified parts, and then diced or sliced ​​to facilitate extraction in the extraction tank 1.

[0038] 2. Extraction:

[0039] Purified water and material were added into extraction tank 1 at a ratio of 1:12. The material liquid was heated to 80°C and extracted for 3-4 hours. After the extraction was completed, the extract was collected.

[0040] 3. Enzymatic hydrolysis:

[0041] The extract was transferred to the enzymolysis tank 5, the temperature was controlled at 55°C and the pH was 7.0, the complex enzyme was added, and the enzymolysis was stirred for 4 hours.

[0042] 4. Fermentation:

[0043] After transferring the enzymatic hydrolyzate to fermentation tank 4, add basal culture medium such as yeast powder and glucose, raise the temperature to 115°C, maintain the temperature for 25 minutes, and sterilize. After sterilization, cool the liquid to 30°C, and transfer the seed liquid in seed tank 3 to fermentation tank 4 through the sterilized pipeline. Maintain the temperature at 30°C and perform anaerobic fermentation for 7 days.

[0044] 5. Filtering:

[0045] After the fermentation is completed, the fermentation liquid is transferred to the heating tank 6, and the temperature is controlled at 80°C for inactivation for 30 minutes. The liquid is then filtered using a filter press 7, and the filtrate is collected through a buffer tank 8.

[0046] 6. Concentration:

[0047] The filtrate was concentrated using a concentrator (60°C, -0.08 MPa) and the reaction was stopped when the solid content reached 15%.

[0048] 7. Enzyme compounding:

[0049] According to the ratio requirements, add vitamins, sugar substitutes and other auxiliary materials to the concentrate, adjust the taste and then fill it in the filling machine.

[0050] However, the above description is merely a specific embodiment of the present invention and should not be used to limit the scope of implementation of the present invention. Therefore, the replacement of equivalent components, or equivalent changes and modifications made according to the scope of protection of the patent of the present invention should still fall within the scope covered by the claims of the present invention.

Claims

1. An enzyme production system, characterized in that: It includes an extraction tank, a stirring tank, a seed tank, a fermentation tank and an enzymolysis tank. There are multiple fermentation tanks and multiple enzymolysis tanks. The material outlet of the extraction tank is connected to the material inlet of the stirring tank, the material outlet of the seed tank is connected to the material inlet of the stirring tank, the material inlet of the fermentation tank is respectively connected to the material outlets of the extraction tank, the stirring tank and the seed tank, the material inlet of the enzymolysis tank is respectively connected to the material outlets of the stirring tank and the seed tank, and the material outlet of the enzymolysis tank is connected to the material inlet of the fermentation tank.

2. An enzyme production system according to claim 1, characterized in that: The fermentation tank includes a reaction inner tube and a jacket, a stirring paddle is provided inside the reaction inner tube, an air duct is provided below the stirring paddle, a material inlet and a cleaning liquid inlet are provided at the upper end of the reaction inner tube, a material outlet, an air inlet and a sewage outlet are provided at the lower end of the reaction inner tube, a hot and cold water outlet is provided at the upper end of the jacket, and a hot and cold water inlet is provided at the lower end of the jacket.

3. An enzyme production system according to claim 1, characterized in that: The multiple fermentation tanks are connected in parallel, and a control valve is provided on the material inlet and outlet pipelines of each fermentation tank.

4. An enzyme production system according to claim 1, characterized in that: The multiple enzymolysis tanks are connected in parallel, and a control valve is provided on the material inlet and outlet pipelines of each enzymolysis tank.

5. An enzyme production system according to claim 1, characterized in that: The enzyme production system also includes a heating tank, a filter press and a buffer tank. The heating tank is provided with a reaction inner cylinder and a jacket. The upper end of the reaction inner cylinder is provided with a material inlet and a cleaning liquid inlet, the lower end of the reaction inner cylinder is provided with a material outlet and a sewage outlet, the upper end of the jacket is provided with a steam inlet, the lower end of the jacket is provided with a steam outlet, the upper end of the buffer tank is provided with a material inlet and a cleaning liquid inlet, and the lower end of the buffer tank is provided with a material outlet and a sewage outlet.

6. An enzyme production system according to claim 5, characterized in that: The material inlet of the heating tank is connected to the material outlet of the fermentation tank or the enzymolysis tank respectively, the material outlet of the heating tank is connected to the material inlet of the filter press, the material outlet of the filter press is connected to the material inlet of the buffer tank, and the material outlet of the buffer tank is connected to the material inlet of the extraction tank.

7. An enzyme production system according to claim 1, characterized in that: The enzyme production system also includes an alkali tank, a water tank and an acid tank, which are connected in parallel. Control valves are provided on the input and output pipelines of the alkali tank, the water tank and the acid tank.

8. An enzyme production system according to claim 7, characterized in that: The liquid outlet of the alkali tank or the acid tank is respectively connected to the cleaning liquid inlet of the extraction tank, stirring tank, seed tank, fermentation tank and enzymolysis tank, and the liquid return port of the alkali tank or the acid tank is respectively connected to the sewage outlet of the extraction tank, stirring tank, seed tank, fermentation tank and enzymolysis tank.

9. An enzyme production system according to claim 1, characterized in that: The enzyme production system also includes a hot water tank and a cold water tank, the liquid outlets of the hot water tank and the cold water tank are respectively connected to the hot and cold water inlets of the seed tank, the fermentation tank and the enzymolysis tank, and the liquid return ports of the hot water tank and the cold water tank are respectively connected to the hot and cold water outlets of the seed tank, the fermentation tank and the enzymolysis tank.