Short-term rumen fluid in-vitro culture fermentation bag for testing influence of medicine on rumen fluid

Abstract

The utility model discloses a short-term rumen fluid in-vitro culture fermentation bag which is used for testing the influence of medicine on rumen fluid, the in-vitro culture fermentation bag comprises a nylon sealing bag with an opening, and the opening is provided with a connector sealed with a rubber plug. The rubber plug comprises a hollow silica gel column (1), an elastic silica gel membrane (2) and a protective metal tube (3) used for preventing the membrane from deforming, and the elastic silica gel membrane (2) is provided with a cross-shaped opening (4) serving as a one-way valve. According to the fermentation bag, the overall sealing performance is guaranteed, an anaerobic environment is created for rumen fluid, the rumen environment is conveniently simulated, growth of rumen contents is facilitated, meanwhile, the specially-structured one-way air guide device is adopted, air in the bag is conveniently exhausted, and the fermentation bag is used for biological experiments.

Description

Technical Field

[0001] The present invention relates to the field of biological fermentation, and in particular to a rubber stopper with a one-way valve for a fermentation bag, which is suitable for short-term in vitro culture of rumen fluid. The rubber stopper is designed to maintain an anaerobic environment in the fermentation bag while facilitating the release of gas during the fermentation process. Background Art

[0002] During in vitro rumen fluid fermentation, gas is produced as a by-product. To simulate the rumen environment, the fermentation bag needs to be kept anaerobic while allowing gas to be discharged and measured.

[0003] Authorized Chinese utility model patent CN220999630U discloses a silage fermentation bag comprising a bag body and a bag lid, wherein one end of the bag body is provided with a bag opening, the bag lid is located at the bag opening and connected to the bag body, the bag lid is provided with a through-exhaust port, the exhaust port is provided with a one-way exhaust member, and the one-way exhaust member comprises a shell and a sealing member, the shell is located within the exhaust port and fixedly connected to the bag lid, the shell has a cavity within the cavity, the cavity is provided with a partition to separate the cavity into an exhaust chamber and an intake chamber, the shell has a plurality of exhaust holes communicating with the exhaust chamber, the shell has a plurality of intake holes communicating with the intake chamber, the partition has a plurality of through-exhaust holes, the sealing member is slidably disposed within the exhaust chamber and abuts against the shell via a closing spring. This utility model can automatically exhaust gases generated by fermentation in the bag, reducing the labor intensity of workers.

[0004] However, existing technologies often lack reliable one-way gas release devices, resulting in reduced accuracy of fermentation experiments. Therefore, the inventors of the utility model conducted research on this problem. Summary of the Invention

[0005] In view of this, the present invention is a rubber stopper system for fermentation bags that can easily discharge gas while maintaining an anaerobic environment in the bag. To achieve the above objectives, the present invention mainly provides the following technical solutions:

[0006] The present invention relates to a short-term in vitro culture fermentation bag for rumen fluid for testing the effects of drugs on rumen fluid. The in vitro culture fermentation bag comprises a nylon sealing bag with an opening, wherein the opening has an interface sealed with a rubber stopper. The invention is characterized in that the rubber stopper comprises a hollow silica gel column (1), an elastic silica gel diaphragm (2) and a protective metal tube (3) for preventing the diaphragm from deforming, and the elastic silica gel diaphragm (2) has a cross opening (4) serving as a one-way valve.

[0007] Preferably, the metal tube (3) is fixed in the center of the hollow silicone column (1) by embedding or snap connection, and the hollow silicone column (1) and the elastic silicone diaphragm (2) are tightly fitted to ensure that the diaphragm can maintain its structural integrity when under pressure.

[0008] Preferably, the elastic silicone diaphragm (2) is located at the center of the hollow silicone column (1) and is tightly connected to the inner wall of the hollow silicone column (1); preferably, the elastic silicone diaphragm (2) is connected to the hollow silicone column (1) by bonding, hot melting, snap-fitting or embedded structure.

[0009] Preferably, the outer diameter of the portion where the rubber stopper meets the bag opening of the nylon sealed bag is 105-115% of the inner diameter of the bag opening, thereby allowing the rubber stopper to be removed to facilitate adding materials into the nylon sealed bag, while also ensuring sealing through elasticity when the rubber stopper is inserted into the bag opening.

[0010] Compared with the prior art, the present invention has the following beneficial effects:

[0011] The fermentation bag of the utility model ensures overall sealing, creates an anaerobic environment for rumen fluid, so as to simulate the rumen environment and is suitable for the growth of rumen contents. At the same time, a specially constructed one-way air guide device is adopted to facilitate the discharge of air in the bag for use in biological experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a structural schematic diagram of a one-way valve rubber stopper for a fermentation bag of the present invention. DETAILED DESCRIPTION

[0013] To further illustrate the technical means and effects employed by the present invention to achieve its intended objectives, the following detailed description of the specific implementation methods, technical solutions, features, and effects of the present invention is provided below, using preferred embodiments and accompanying drawings. The specific features, structures, or characteristics of the various embodiments described below may be combined in any suitable manner.

[0014] Example 1

[0015] The in vitro culture fermentation bag of the utility model comprises a nylon sealing bag with an opening ( Figure 1(not shown), the opening has an interface sealed with a rubber stopper, the rubber stopper includes a hollow silica gel column (1), an elastic silica gel diaphragm (2) and a protective metal tube (3) for preventing the diaphragm from deforming, and the elastic silica gel diaphragm (2) has a cross opening (4) as a one-way valve. The cross opening (4) in the rubber stopper is used to insert a syringe to extract gas and collect samples in the rumen fluid fermentation experiment. The fermentation bag is mainly used to evaluate the effect of drugs on rumen fluid. Its technical principle is to perform artificial in vitro culture on fresh rumen fluid in a short period of time. Before the rumen fluid is put into the bag, Chinese medicine or other drugs can be added. Subsequently, the fermentation bag containing rumen fluid is placed in a water bath shaker or artificial rumen incubator that has been preheated to 39°C. During the culture process, the rumen fluid will produce gas. When needed, the experimenter can connect the rubber stopper with a syringe to extract gas from the bag and record the gas production of the rumen fluid. After the culture is completed, the rumen fluid in the bag can be used to test other rumen fermentation parameters, such as pH, microbial protein (MCP), volatile fatty acids (VFA), etc., to evaluate the specific effects of drugs on the rumen fluid.

[0016] The hollow silica gel column (1) is made of silica gel to ensure flexibility and resistance to chemicals used in the fermentation process, and the hollow structure provides a channel for gas to be discharged.

[0017] Protective support metal tube (2): A rigid metal tube is placed around the silicone diaphragm to provide structural support and prevent the diaphragm from being flattened or deformed when external pressure is applied. This metal tube ensures that the valve maintains its shape and functions properly over repeated uses. The metal tube is fixed to the center of the hollow silicone column by embedding or snap-fitting. It fits tightly with the silicone column and the elastic diaphragm, ensuring that the diaphragm maintains its structural integrity when under pressure.

[0018] Elastic silicone diaphragm (3): Located within the hollow silicone column, the cross-shaped opening (4) in the diaphragm controls the release of gas. When the air pressure in the fermentation bag increases, the diaphragm opens to release the gas, and when the pressure drops, it closes to prevent air from entering, thereby maintaining the anaerobic environment within the bag. The elasticity of the diaphragm ensures that it works effectively under different pressures and does not permanently deform. The elastic diaphragm is located in the center of the hollow silicone column and is tightly bonded to the inner wall of the silicone column. The diaphragm can be connected to the main body by bonding, hot melting, snap-fitting or embedded structure to ensure that it does not shift under fluid pressure.

[0019] The above disclosure is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any modifications or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in this invention should be included in the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be based on the scope of protection of the above claims.

Claims

1. A short-term in vitro culture and fermentation bag for testing the effects of drugs on rumen fluid, the in vitro culture and fermentation bag comprising a nylon sealed bag with an opening, the opening having an interface sealed with a rubber stopper, characterized in that: The rubber stopper comprises a hollow silicone column (1), an elastic silicone diaphragm (2) and a protective metal tube (3) for preventing the diaphragm from deforming. The elastic silicone diaphragm (2) has a cross opening (4) serving as a one-way valve.

2. The in vitro culture fermentation bag according to claim 1, characterized in that: The protective metal tube (3) is fixed at the center of the hollow silicone column (1) by embedding or snap connection, and the hollow silicone column (1) and the elastic silicone diaphragm (2) are tightly fitted.

3. The in vitro culture fermentation bag according to claim 1, characterized in that: The elastic silicone membrane (2) is located at the center of the hollow silicone column (1) and is tightly connected to the inner wall of the hollow silicone column (1).

4. The in vitro culture fermentation bag according to claim 3, characterized in that: The elastic silicone membrane (2) is connected to the hollow silicone column (1) by bonding, hot melting, snap-fitting or an embedded structure.

5. The in vitro culture fermentation bag according to claim 1, characterized in that: The outer diameter of the portion where the rubber stopper meets the bag opening of the nylon sealing bag is 105-115% of the inner diameter of the bag opening.

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