Gene high-throughput sequencing device

Abstract

The gene high-throughput sequencing device comprises a bottom frame and a reagent storage device on the upper side of the bottom frame, the bottom frame comprises a supporting frame and a first motor vertically arranged on the supporting frame in a sliding mode, and a transmission shaft of the first motor faces upwards. The reagent storage device comprises a chassis and a storage barrel, the upper side of the storage barrel is driven by a second motor to rotate, a sealing cover is further arranged on the upper side of the storage barrel, the middle of the lower side of the chassis is hinged to the upper end of the transmission shaft, and an adjusting mechanism capable of adjusting the inclination angle of the chassis is arranged between the chassis and the transmission shaft; the storage cylinder can be driven to rotate around the vertical axis in an inclined state and driven by the second motor to rotate around the axis of the storage cylinder, shaking work of an experimental sample in the storage cylinder is achieved, no stirring instrument needs to be arranged in the storage cylinder, the uniformity of the experimental sample can be guaranteed, and meanwhile under the effect of a sealing cover, the experimental sample can be conveniently stored. The loss of the temperature of the experimental sample in the shaking process is effectively prevented, the shaking stirring degree can be adjusted as required only through the adjusting mechanism, and the control process is simpler and more convenient.

Description

Technical Field

[0001] The utility model relates to the technical field of gene sequencing, in particular to a high-throughput gene sequencing device. Background Art

[0002] Gene sequencing is a new genetic testing technology that can analyze and determine the full gene sequence from samples such as blood or saliva, and then interpret these different sequences through bioinformatics analysis to predict the possibility of suffering from various diseases, as well as individual behavioral characteristics and behavioral rationality.

[0003] The patent document with application number CN202222404340.3 in the patent library discloses a high-throughput gene sequencing device, which drives several stirring rods to rotate at high speed by the user shaking the handwheel, thereby stirring the experimental samples inside the container at high speed. This stirring method requires the stirring rod to be fully extended into the container, which can easily contaminate the experimental samples, and the subsequent cleaning of the stirring rod is also more troublesome.

[0004] For example, the patent document with application number CN202120284116.8 in the patent library discloses a high-throughput gene sequencing device, which completes the circulation of reagents in the tank by setting a circulation pump, thereby ensuring the uniformity of the reagents. However, since the reagents need to maintain a certain temperature, this external circulation pump to circulate the reagents externally is bound to have an adverse effect on the actual temperature. Utility Model Content

[0005] In order to solve the technical problems existing in the above-mentioned background technology, the present invention provides a high-throughput gene sequencing device.

[0006] The technical solution of this utility model is as follows:

[0007] A high-throughput gene sequencing device comprises a base frame and a reagent storage arranged on the upper side thereof, and the storage of experimental samples can be completed through the reagent storage.

[0008] As one of the core technical concepts of the present invention, the base frame includes a support frame and a first motor vertically slidingly arranged thereon, and the transmission shaft of the first motor faces upward; as another core technical concept of the present invention, the reagent storage device includes a base and a storage cylinder on its upper side driven to rotate by a second motor, and a cover is also provided on the upper side of the storage cylinder. The middle part of the lower side of the base is hinged to the upper end of the transmission shaft, and an adjustment mechanism capable of adjusting its inclination angle is provided between the base and the transmission shaft. On the basis of the above structure, through the action of the first motor, the storage cylinder can be driven to rotate around the vertical axis in a tilted state, and rotate around its axis under the drive of the second motor to achieve shaking of the experimental sample inside it. There is no need to set any stirring device inside the storage cylinder, which can ensure the uniformity of the experimental sample. At the same time, under the action of the cover, the experimental sample is shaken inside the sealed storage cylinder, effectively preventing the loss of temperature of the experimental sample during the shaking process. Moreover, it is only necessary to control the inclination angle of the storage cylinder through the adjustment mechanism, so that the degree of shaking and stirring can be adjusted as needed, and the control process can be simpler and more convenient.

[0009] In the high-throughput gene sequencing device as described above, in order to ensure the shaking effect of the test sample and thereby improve its uniformity, the driving rotation directions of the first motor and the second motor are opposite.

[0010] In order to ensure that the sequencing device (the above-mentioned high-throughput gene sequencing device) can be adapted to the shaking and stirring of experimental samples under different requirements, the speed of the first motor and / or the second motor is adjustable.

[0011] In the high-throughput gene sequencing device as described above, in order to ensure that the storage cylinder can better stir the experimental samples inside it when it moves under the action of the first motor and the second motor, a first strip-shaped guide vane is provided on the inner wall of the storage cylinder along its axial direction.

[0012] In order to further ensure the stirring effect of the first guide vane, a plurality of the first guide vanes are arranged in a circular array around the axis of the storage cylinder.

[0013] As a preferred embodiment, in order to ensure that the experimental sample at the bottom can be effectively stirred when the storage cylinder is in operation to prevent the experimental sample from settling, the storage cylinder bottom has a plurality of strip-shaped second guide vanes arranged in a circumferential array perpendicular to its axial direction.

[0014] In the high-throughput gene sequencing device described above, in order to prevent the experimental samples from spilling out of the storage tube during shaking while ensuring the storage capacity of the experimental samples in the storage tube, an annular anti-overflow plate is provided on the inner ring of the open end of the storage tube.

[0015] As described above, a high-throughput gene sequencing device, specifically speaking, includes a clamping bolt and an adjustment plate arranged on the lower side of the chassis; the adjustment plate is provided with an arc-shaped adjustment opening whose center coincides with the rotation axis of the chassis, the clamping bolt passes through the adjustment opening and is spirally connected to the transmission shaft, and the chassis tilt angle can be adjusted by loosening or tightening the clamping bolt, making the adjustment process simpler and more convenient.

[0016] As a preferred embodiment, in order to prevent the connection between the base frame, the reagent storage device and the adjustment mechanism from being destroyed when there are a large number of experimental samples, the support frame is configured as a cylindrical structure, the first motor is vertically slidably arranged in the support frame, and the lower side of the outer edge of the chassis is in contact with the upper outer ring of the support frame.

[0017] The beneficial effects of the present invention are as follows: the present invention is a high-throughput gene sequencing device, which can drive the storage barrel to rotate around the vertical axis in an inclined state through the action of the first motor, and rotate around its axis under the drive of the second motor, thereby shaking the experimental sample inside it. There is no need to set any stirring device inside the storage barrel, which can ensure the uniformity of the experimental sample; at the same time, under the action of the cap, the experimental sample is shaken inside the storage barrel in a sealed state, effectively preventing the loss of the experimental sample temperature during the shaking process; moreover, only the tilt angle of the storage barrel needs to be controlled by the adjustment mechanism, so that the shaking and stirring degree can be adjusted according to needs, and the control process can be simpler and more convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] By reading the detailed description of the preferred embodiment below, the solutions and advantages of the present application will become clear to those skilled in the art. The accompanying drawings are only used to illustrate the preferred embodiment and are not to be considered as limiting the present invention.

[0019] In the attached figure:

[0020] Figure 1 Schematic diagram of the structure of the sequencing device in the embodiment;

[0021] Figure 2 for Figure 1 The main view;

[0022] Figure 3 Schematic diagram of the structure of the chassis in the embodiment;

[0023] Figure 4 Schematic diagram of the internal structure of the storage cylinder in the embodiment;

[0024] Figure 5 Schematic diagram of the structure of the adjustment mechanism in the embodiment;

[0025] The components represented by the reference numerals in the figure are:

[0026] 1. Base frame; 11. Support frame; 12. First motor; 121. Body; 122. Drive shaft; 123. Guide plate; 13. Clamp; 2. Reagent storage device; 21. Chassis; 22. Storage cylinder; 23. Cover; 24. First guide vane; 25. Second guide vane; 26. Anti-overflow plate; 3. Adjustment mechanism; 31. Adjustment plate; 32. Holding bolt. DETAILED DESCRIPTION

[0027] Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings.

[0028] Example

[0029] This embodiment provides a high-throughput gene sequencing device, see Figure 1 and Figure 2 , including a base frame 1 and a reagent storage 2 arranged on its upper side, and the reagent storage 2 can complete the storage of experimental samples. The structure of the sequencing device (the above-mentioned high-throughput gene sequencing device) is described in detail below.

[0030] In this embodiment, as one of the core technical concepts of the present invention, the base frame 1 includes a support frame 11 and a first motor 12 vertically slidingly arranged thereon, and the transmission shaft 122 of the first motor 12 faces upward; as another core technical concept of the present invention, the reagent storage device 2 includes a circular chassis 21, and a cylindrical storage tube 22 coaxially arranged on the upper side of the chassis 21 and driven to rotate by a second motor, and a cover 23 is also provided on the upper side of the storage tube 22. The middle part of the lower side of the chassis 21 is hinged to the upper end of the transmission shaft 122, and its rotation axis around the hinged position is horizontal, and an adjustment mechanism capable of adjusting its inclination angle is provided between the chassis 21 and the transmission shaft 122. 3. On the basis of the above structure, the action of the first motor 12 can drive the storage barrel 22 to rotate around the vertical axis in an inclined state, and rotate around its axis under the drive of the second motor, so as to achieve the shaking of the experimental sample therein. There is no need to set any stirring device inside the storage barrel 22, which can ensure the uniformity of the experimental sample. At the same time, under the action of the cover 23, the experimental sample is shaken inside the sealed storage barrel 22, which effectively prevents the loss of the experimental sample temperature during the shaking process. Moreover, it is only necessary to control the inclination angle of the storage barrel 22 through the adjustment mechanism 3, so that the shaking and stirring degree can be adjusted as needed, and the control process can be simpler and more convenient.

[0031] In this embodiment, combined with Figure 3 and Figure 5 As for the structure of the base frame 1 , the support frame 11 is configured as a cylindrical tube structure, and the first motor 12 is vertically slidably disposed in the support frame 11 .

[0032] Specifically, the base frame 1 also includes two plywood 13 arranged in a gap relative to each other in the support frame 11, and the middle part of the plywood 13 is an arc-shaped structure protruding toward one side away from each other. The first motor 12 includes a vertically arranged body 121, and the transmission shaft 122 is vertically arranged at the upper end of the body 121. Guide plates 123 are also provided on both sides of the body 121. The body 121 is vertically slid between the arc-shaped protrusions of the two plywood 13, and the guide plates 123 on both sides are vertically arranged between the two plywood 13, and the two sides are in contact with the two plywood 13 so that the first motor 12 can only slide vertically between the two plywood 13. On the basis of the above structure, in order to prevent the connection relationship between the base frame 1, the reagent storage device 2 and the adjustment mechanism 3 from being destroyed when there are a large number of experimental samples, the lower side of the outer edge of the chassis 21 is in contact with the outer ring of the upper end of the support frame 11.

[0033] As a preferred embodiment of the present invention, in order to ensure the shaking effect of the test sample and thus improve its uniformity, the driving rotation directions of the first motor 12 and the second motor are opposite.

[0034] As a further preference, in order to ensure that the sequencing device can be suitable for shaking and stirring experimental samples under different requirements, the speed of the first motor 12 and / or the second motor is adjustable. Preferably, the first motor 12 and the second motor are both servo motors, and the speed is adjustable.

[0035] In this embodiment, combined with Figure 4 In order to ensure that the storage cylinder 22 can better stir the experimental sample inside it when it moves under the action of the first motor 12 and the second motor, a first guide vane 24 in the form of a strip is provided on the inner wall of the storage cylinder 22 along its axial direction.

[0036] To further ensure the stirring effect of the first guide vanes 24 , a plurality of first guide vanes 24 are arranged in a circular array around the axis of the storage barrel 22 .

[0037] As a preferred embodiment, in order to ensure that the experimental sample at the bottom can be effectively stirred when the storage cylinder 22 is in operation to prevent the experimental sample from sedimentation, the storage cylinder 22 has a plurality of strip-shaped second guide vanes 25 arranged perpendicular to its axial direction in a circumferential array at the bottom.

[0038] More preferably, in order to prevent the experimental sample from spilling out of the storage cylinder 22 during shaking and to ensure the storage capacity of the experimental sample in the storage cylinder 22, an annular anti-overflow plate 26 is further provided on the inner ring of the open end of the storage cylinder 22.

[0039] In this embodiment, combined with Figure 5 Specifically speaking, the structure of the adjustment mechanism 3 includes a clamping bolt 32 and an adjustment plate 31 provided on the lower side of the chassis 21.

[0040] Specifically, the adjustment plate 31 is an arc-shaped strip structure, both ends of which are fixed to the lower side of the chassis 21, and an arc-shaped adjustment opening is opened in the middle, the center of the circle coincides with the rotation axis of the chassis 21. The clamping bolt 32 passes through the adjustment opening and is spirally connected to the transmission shaft 122. By loosening and tightening the clamping bolt 32, the inclination angle of the chassis 21 can be adjusted, and the adjustment process is simpler and more convenient.

Claims

1. A high-throughput gene sequencing device, characterized in that: It comprises a base frame (1) and a reagent storage (2) arranged on its upper side; The base frame (1) comprises a support frame (11) and a first motor (12) vertically slidably arranged thereon, wherein a transmission shaft (122) of the first motor (12) faces upward; The reagent storage device (2) comprises a chassis (21) and a storage cylinder (22) on its upper side which is driven to rotate by a second motor. A sealing cover (23) is also provided on the upper side of the storage cylinder (22). The middle portion of the lower side of the chassis (21) is hinged to the upper end of the transmission shaft (122), and an adjustment mechanism (3) capable of adjusting the inclination angle thereof is provided between the chassis (21) and the transmission shaft (122).

2. A high-throughput gene sequencing device according to claim 1, characterized in that: The driving rotation directions of the first motor (12) and the second motor are opposite.

3. A high-throughput gene sequencing device according to claim 1, characterized in that: The rotation speed of the first motor (12) and / or the second motor is adjustable.

4. A high-throughput gene sequencing device according to claim 1, characterized in that: A strip-shaped first guide vane (24) is provided on the inner wall of the storage cylinder (22) along the axial direction thereof.

5. A high-throughput gene sequencing device according to claim 4, characterized in that: There are a plurality of first guide vanes (24) arranged in a circular array around the axis of the storage barrel (22).

6. A high-throughput gene sequencing device according to claim 4, characterized in that: The bottom circumferential array of the storage cylinder (22) is provided with a plurality of strip-shaped second guide vanes (25) arranged perpendicular to the axial direction thereof.

7. A high-throughput gene sequencing device according to claim 1, characterized in that: An annular anti-overflow plate (26) is provided on the inner ring of the open end of the storage cylinder (22).

8. A high-throughput gene sequencing device according to any one of claims 1 to 7, characterized in that: The adjustment mechanism (3) includes a clamping bolt (32) and an adjustment plate (31) provided on the lower side of the chassis (21); The adjusting plate (31) is provided with an arc-shaped adjusting opening whose center coincides with the rotation axis of the chassis (21), and the clamping bolt (32) passes through the adjusting opening and is spirally connected to the transmission shaft (122).

9. A high-throughput gene sequencing device according to claim 8, characterized in that: The support frame (11) is a cylindrical structure, the first motor (12) is vertically slidably arranged in the support frame (11), and the lower side of the outer edge of the chassis (21) contacts the upper outer ring of the support frame (11).

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