Sample blending device and single cell printer

Abstract

The utility model provides a sample mixing device and a single cell printer. The sample mixing device comprises a pipeline unit, a sample storage unit, a liquid collecting unit, a mixing unit, a concentration measuring unit and a control unit. An inlet of a liquid input pipeline of the pipeline unit is used for inputting sample liquid, and one side of the liquid input pipeline is communicated with an outlet of a gas input pipeline of the pipeline unit; the uniform mixing unit is connected with an inlet of the gas input pipeline; an inlet of the sample storage unit is communicated with an outlet of the liquid input pipeline, and sample liquid flows into the sample storage unit through the inlet of the sample storage unit; the liquid collecting unit can collect sample liquid drops ejected from the outlet of the sample storage unit; the concentration measuring unit is arranged in the liquid collecting unit and is used for measuring the concentration of the sample liquid drops; the control unit is used for generating a uniform mixing control signal according to the concentration and sending the uniform mixing control signal to the uniform mixing unit so as to control the gas input pipeline to perform reciprocating gas suction on the sample liquid in the liquid input pipeline and ensure that the sample liquid flowing into the sample storage unit is in a uniform mixing state.

Description

Technical Field

[0001] The utility model relates to the technical field of microfluidics, in particular to a sample mixing device and a single cell printer. Background Art

[0002] Single-cell sorting can achieve the capture, separation and analysis of any single type of cell or rare cell in a suspension system (such as circulating tumor cells, endothelial cells, stem cells, and related cells in blood and bone marrow, etc.). Existing single-cell sorting mostly uses the separation dilution method, which disperses single cells in the culture medium by diluting the cell suspension multiple times, continuously reducing the cell concentration, and ultimately achieving single-cell sorting. This method requires a lot of time, manpower and material resources, is costly, has low sorting efficiency, and may cause non-single cell sorting.

[0003] In addition, since cells in the sample liquid are very prone to sedimentation, cell sedimentation can cause blockage in the inflowing microfluidic channel, thereby affecting the normal operation of the cell sorting process and the final efficiency and accuracy of the optical detection results of the sorted sample droplets. Utility Model Content

[0004] The present application provides a sample mixing device and a single-cell printer, which aim to solve the problem that cells in the sample liquid are very prone to sedimentation, and cell sedimentation can cause blockage in the flowing microfluidic channel, thereby affecting the normal operation of the cell sorting process and the final efficiency and accuracy of the optical detection results of the sorted sample droplets.

[0005] In a first aspect, the present application provides a sample mixing device, comprising:

[0006] A pipeline unit, comprising a liquid input pipeline and a gas input pipeline, wherein the inlet of the liquid input pipeline is used to input the sample liquid, and one side of the liquid input pipeline is connected to the outlet of the gas input pipeline;

[0007] a mixing unit connected to the inlet of the gas input pipeline;

[0008] a sample storage unit, wherein the inlet of the sample storage unit is connected to the outlet of the liquid input pipeline, and the sample liquid flows into the cell fluid cavity of the sample storage unit through the inlet of the sample storage unit;

[0009] a liquid collecting unit, configured to collect sample droplets ejected from the outlet of the sample storage unit;

[0010] a concentration measuring unit, the concentration measuring unit being disposed in the liquid collecting unit and measuring the concentration of the sample droplets in the liquid collecting unit;

[0011] a control unit electrically connected to the concentration measurement unit and the mixing unit, the concentration measurement unit sending the concentration of the sample droplet to the control unit, the control unit generating a mixing control signal according to the concentration and sending it to the mixing unit, the mixing unit controlling the gas input pipeline to perform reciprocating gas suction on the sample liquid in the liquid input pipeline according to the mixing control signal, so as to ensure that the sample liquid flowing into the sample storage unit is in a mixed state.

[0012] In some embodiments, the mixing unit includes: a first air pump, the first air pump is connected to the inlet of the gas input pipeline, the control unit is electrically connected to the first air pump, the control unit generates the mixing control signal according to the concentration and sends it to the first air pump, and the first air pump performs reciprocating gas suction on the sample liquid according to the mixing control signal.

[0013] Exemplarily, a preset solenoid valve is provided between the first air pump and the gas input pipeline, and the preset solenoid valve is located above the first air pump.

[0014] It should be noted that, in some embodiments, the control unit is electrically connected to the preset solenoid valve, and the mixing control signal includes an air pump on-control signal, an air pump off-control signal, a solenoid valve on-control signal and a solenoid valve off-control signal; the control unit sends the air pump on-control signal to the first air pump and sends the solenoid valve off-control signal to the preset solenoid valve; or, the control unit sends the air pump off-control signal to the first air pump and sends the solenoid valve on-control signal to the preset solenoid valve.

[0015] In some embodiments, the sample storage unit includes: a substrate, the substrate including a cell fluid cavity, the cell fluid cavity being used to place the sample liquid, the cell fluid cavity including a liquid inlet and a liquid outlet, the liquid inlet of the cell fluid cavity being connected to the outlet of the liquid input pipeline; a first electrode, the first electrode being arranged on the substrate and located outside the cell fluid cavity; a piezoelectric substrate, the piezoelectric substrate being made of piezoelectric material, the piezoelectric substrate being arranged on the first electrode; a second electrode, the second electrode being arranged on the piezoelectric substrate, the second electrode having opposite polarity to the first electrode; wherein the control unit is connected to the first electrode and the second electrode, the control unit generates a second control signal to the first electrode and the second electrode, and the second electrode and the first electrode drive the piezoelectric substrate to vibrate according to the second control signal to propagate an acoustic pulse wave in the sample liquid in the cell fluid cavity, thereby driving the sample liquid to vibrate to form sample droplets, and causing the sample droplets to be ejected through the liquid outlet.

[0016] Illustratively, the cross-sectional shapes of the first electrode, the second electrode, and the piezoelectric substrate relative to the flow direction of the sample liquid are the same as the cross-sectional shape of the cell fluid cavity relative to the flow direction of the sample liquid.

[0017] Exemplarily, the length of the first electrode relative to the flow direction of the sample liquid is a preset electrode length, and the length of the piezoelectric material relative to the flow direction of the sample liquid is a preset material length; the preset electrode length is greater than or equal to the preset material length.

[0018] Illustratively, the substrate includes a first substrate and a second substrate, the first electrode is disposed on a first side of the first substrate, and the second side of the first substrate is connected to the first side of the second substrate to form the cell fluid cavity.

[0019] Exemplarily, the acoustic pulse wave is a single pulse signal with a pulse width of 0.5μs to 20μs, a peak-to-peak voltage in the range of 0-500V, and a pulse amplitude of the first pulse amplitude; or, the acoustic pulse wave is a double pulse signal or a bipolar pulse signal with a pulse width of 1μs to 3μs, a pulse interval of 4μs to 30us, and a pulse amplitude of the second pulse amplitude, and the second pulse amplitude is smaller than the first pulse amplitude.

[0020] In a second aspect, an embodiment of the present application provides a single-cell printer, comprising the sample mixing device provided in any embodiment of the present application.

[0021] The present application provides a sample mixing device and a single-cell printer. The sample mixing device includes: a pipeline unit, a sample storage unit, a liquid collection unit, a mixing unit, a concentration measurement unit and a control unit. The pipeline unit includes a liquid input pipeline and a gas input pipeline, wherein the inlet of the liquid input pipeline is used to input sample liquid, and one side of the liquid input pipeline is connected to the outlet of the gas input pipeline; the mixing unit is connected to the inlet of the gas input pipeline; the inlet of the sample storage unit is connected to the outlet of the liquid input pipeline, and the sample liquid flows into the cell fluid cavity of the sample storage unit through the inlet of the sample storage unit; the liquid collection unit is used to collect sample droplets ejected from the outlet of the sample storage unit; the concentration measuring unit is arranged in the liquid collection unit and measures the concentration of the sample droplets in the liquid collection unit; the control unit is electrically connected to the concentration measuring unit and the mixing unit, the concentration measuring unit sends the concentration of the sample droplets to the control unit, the control unit generates a mixing control signal based on the concentration and sends it to the mixing unit, and the mixing unit controls the gas input pipeline to perform reciprocating gas suction on the sample liquid in the liquid input pipeline according to the mixing control signal to ensure that the sample liquid flowing into the sample storage unit is in a mixed state. By ensuring that the sample liquid is in a mixed state during the cell sorting process, blockage in the microfluidic channel flowing into the sample storage unit due to cell sedimentation can be avoided, ensuring the normal operation of the cell sorting process and the efficiency and accuracy of the optical detection results of the sorted sample droplets. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following is a brief introduction to the drawings required for use in the description of the embodiments. Obviously, the drawings described below are some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0023] Figure 1 This is a schematic structural diagram of a sample mixing device provided in an embodiment of the present application;

[0024] Figure 2 This is a schematic structural diagram of a sample storage unit provided in an embodiment of the present application;

[0025] Figure 3 Schematic diagram of the structure of the single-cell printer provided in an embodiment of the present application.

[0026] Description of main components and symbols:

[0027] 100. Single cell printer;

[0028] 10. Sample mixing device

[0029] 11. Pipeline unit; 111. Liquid input pipeline; 112. Gas input pipeline; 12. Sample storage unit; 121. Substrate; 1211. Cell fluid chamber; 1212. First substrate; 1213. Second substrate; 122. First electrode; 123. Piezoelectric substrate; 124. Second electrode; 13. Liquid collection unit; 14. Mixing unit; 141. First air pump; 142. Preset solenoid valve; 15. Control unit;

[0030] 20. Sample droplets.

[0031] It should be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the present application. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are part of the embodiments of this application, not all of them. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0033] The flowcharts shown in the accompanying drawings are for illustrative purposes only and do not necessarily include all contents and operations / steps, nor must they be executed in the order described. For example, some operations / steps may be decomposed, combined, or partially merged, so the actual execution order may vary depending on the actual situation.

[0034] It should be understood that the terms used in this specification are only for the purpose of describing specific embodiments and are not intended to limit the present application. As used in this specification and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms unless the context clearly indicates otherwise.

[0035] It should be understood that, in order to facilitate a clear description of the technical solutions of the embodiments of the present application, in the embodiments of the present application, words such as "first" and "second" are used to distinguish between identical or similar items with substantially the same functions and effects. For example, the first support member and the second support member are merely used to distinguish between different support members and do not limit their order. Those skilled in the art will understand that words such as "first" and "second" do not limit the quantity or execution order, and words such as "first" and "second" do not necessarily limit them to be different.

[0036] It should be further understood that the term “and / or” used in this specification and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes these combinations.

[0037] The following describes some embodiments of the present application in detail with reference to the accompanying drawings. In the absence of conflict, the following embodiments and features therein may be combined with each other.

[0038] With the continuous development of cell therapy and gene therapy technologies, cell-related research has gradually become a research hotspot in the current medical and life science fields. Cell culture has become a very important technology in scientific research. Cell sorting and purification are the key to obtaining purer target cells.

[0039] Single-cell sorting can achieve the capture, separation and analysis of any single type of cell or rare cell in a suspension system (such as circulating tumor cells, endothelial cells, stem cells, and related cells in blood and bone marrow, etc.). Existing single-cell sorting mostly uses the separation dilution method, which disperses single cells in the culture medium by diluting the cell suspension multiple times, continuously reducing the cell concentration, and ultimately achieving single-cell sorting. This method requires a lot of time, manpower and material resources, is costly, has low sorting efficiency, and may cause non-single cell sorting.

[0040] In addition, since cells in the sample liquid are very prone to sedimentation, cell sedimentation can cause blockage in the inflowing microfluidic channel, thereby affecting the normal operation of the cell sorting process and the final efficiency and accuracy of the optical detection results of the sorted sample droplets.

[0041] To solve the above problems, please refer to Figure 1 , Figure 1 Schematic diagram of the structure of the sample mixing device 10 provided in the embodiment of the present application. Figure 1As shown, the provided sample mixing device 10 includes: a pipeline unit 11, a sample storage unit 12, a liquid collection unit 13, a mixing unit 14, a concentration measurement unit and a control unit 15. The pipeline unit 11 includes a liquid input pipeline 111 and a gas input pipeline 112. The inlet of the liquid input pipeline 111 is used to input sample liquid, and one side of the liquid input pipeline 111 is connected to the outlet of the gas input pipeline 112; the mixing unit 14 is connected to the inlet of the gas input pipeline 112; the inlet of the sample storage unit 12 is connected to the outlet of the liquid input pipeline 111, and the sample liquid flows into the cell fluid cavity of the sample storage unit 12 through the inlet of the sample storage unit 12; the liquid collection unit 13 is used to collect sample droplets ejected from the outlet of the sample storage unit; the concentration measurement unit is provided In the liquid collection unit 13, the concentration of the sample droplets in the liquid collection unit 13 is measured; a control unit 15 is electrically connected to the concentration measurement unit and the mixing unit 14. The concentration measurement unit sends the concentration of the sample droplets to the control unit 15, which generates a mixing control signal based on the concentration and sends it to the mixing unit 14. The mixing unit 14 controls the gas input line 112 to perform reciprocating gas suction on the sample liquid in the liquid input line 111 according to the mixing control signal, thereby ensuring that the sample liquid flowing into the sample storage unit 12 is in a mixed state. The provided device ensures that the sample liquid is in a mixed state during the cell sorting process, avoids blockage in the microfluidic channel flowing into the sample storage unit 12 due to cell sedimentation, ensures the normal operation of the cell sorting process, and ensures the efficiency and accuracy of the optical detection results of the sorted sample droplets.

[0042] Specifically, existing cell sampling equipment is mostly based on a preset power input. When the cells in the input suspension settle, it will lead to the input of too many cells. After all cells are input, the amount of cells input subsequently decreases, thereby making the cell concentration of the sample liquid in the sample storage unit 12 extremely uneven, making it difficult for the sample storage unit 12 to ensure that all sorted sample droplets 20 include cells.

[0043] In some embodiments, as Figure 1 As shown, the mixing unit 14 includes a first air pump 141, which is connected to the inlet of the gas input pipeline 112; wherein, the control unit 15 is electrically connected to the first air pump 141, and the control unit 15 generates the mixing control signal according to the concentration and sends it to the first air pump 141, and the first air pump 141 performs reciprocating gas suction on the sample liquid according to the mixing control signal.

[0044] For example, Figure 1 As shown, a preset solenoid valve 142 is provided between the first air pump 141 and the gas input pipeline 112 , and the preset solenoid valve 142 is located above the first air pump 141 .

[0045] It should be noted that in some embodiments, the control unit 15 is electrically connected to the preset solenoid valve 142, and the mixing control signal includes an air pump on control signal, an air pump off control signal, a solenoid valve on control signal, and a solenoid valve off control signal. The control unit 15 sends the air pump on control signal to the first air pump 141 and the solenoid valve off control signal to the preset solenoid valve 142; or the control unit 15 sends the air pump off control signal to the first air pump 141 and the solenoid valve on control signal to the preset solenoid valve 142. This allows the first air pump 141 to aspirate gas from the sample liquid when the preset solenoid valve 142 is closed, and the gas aspirated from the sample liquid by the first air pump 141 re-enters the sample liquid in the liquid input line 111 through the gas input line 112 when the preset solenoid valve 142 is opened, thereby ensuring that the sample liquid flowing into the sample storage unit 12 is in a mixed state.

[0046] Exemplarily, the control parameters include the pumping power and frequency of the first air pump 141. When the cell concentration is lower than a preset concentration, the control unit 15 adjusts the control parameters to reduce the pumping power and frequency of the first air pump 141 to increase the cell concentration. When the cell count is higher than the preset concentration, the control unit 15 adjusts the control parameters to increase the pumping power and frequency of the first air pump 141 to reduce the cell concentration. When the cell concentration is equal to the preset concentration, the control unit 15 does not adjust the control parameters. The provided method can thus adjust the control parameters in real time based on the cell concentration.

[0047] In some embodiments, as Figure 2As shown, the sample storage unit 12 includes a substrate 121, a first electrode 122, a piezoelectric substrate 123, and a second electrode 124. The substrate 121 includes a cell fluid cavity 1211, which is used to hold the sample liquid to be sorted. The cell fluid cavity 1211 includes a liquid inlet and a liquid outlet, and the liquid inlet is connected to the pipeline unit 11. The optical detection device 13 faces the substrate 121 and is opposite to the cell fluid cavity 1211. The first electrode 122 is disposed on the substrate 121 and is located outside the cell fluid cavity 1211. The piezoelectric substrate 123 is made of piezoelectric material and is disposed on the first electrode 122. The second electrode 124 is disposed on the piezoelectric substrate 123, and the polarity of the second electrode 124 is opposite to that of the first electrode 122. The second electrode 124 and the first electrode 122 are electrically connected to the control unit 15. The control unit 15 is configured to control the first and second electrodes 122, 124 to drive the piezoelectric substrate 123 to vibrate, thereby propagating acoustic pulse waves in the sample liquid within the cell fluid chamber 1211. This drives the sample liquid to vibrate, forming sample droplets 20, which are then ejected through the liquid outlet. Consequently, the provided single-cell printer 10 can reduce cell damage during the cell sorting process and improve experimental accuracy.

[0048] Exemplarily, the transmittance of the first substrate is greater than a preset transmittance.

[0049] For example, Figure 2 As shown, the substrate 121 includes a first substrate 1212 and a second substrate 1213. The first electrode 122 is disposed on a first side of the first substrate 1212. The second side of the first substrate 1212 is connected to the first side of the second substrate 1213 to form a cell fluid cavity 1211. The transmittance of the first substrate 1212 is greater than a predetermined transmittance. The optical detection device 13 faces the first substrate 1212 and is opposite to the cell fluid cavity 1211.

[0050] It should be noted that in some embodiments, the first substrate 1212 may be made of a light-transmitting material such as glass, and the second substrate may be made of silicon. In this case, light from the optical detection device 13 will enter the cell fluid cavity 1211 through the first substrate 1212 and then be reflected back by the second substrate 1213. If the second substrate 1213 is also made of a light-transmitting material, the optical detection device needs to be provided with a receiving device opposite to the second substrate 1213.

[0051] It should be noted that, in some embodiments, a solution tank is provided on the first side of the second substrate 1213 . When the second side of the first substrate 1212 is connected to the first side of the second substrate 1213 , the solution tank and the second side of the first substrate 1212 form a cell fluid cavity 1211 .

[0052] It should be noted that, in some embodiments, the liquid inlet is provided on the second side surface of the second substrate 1213 ; the solution tank includes a liquid outlet tank, and the liquid outlet tank and the second side surface of the first substrate 1212 constitute the liquid outlet.

[0053] It should be noted that in some embodiments, the length of the first electrode 122 relative to the sample liquid flow direction is a preset electrode length, and the length of the piezoelectric material 123 relative to the sample liquid flow direction is a preset material length. The preset electrode length is greater than or equal to the preset material length.

[0054] In the method provided in the present application, the first electrode 122 must cover the piezoelectric material 123 in the direction of sample liquid flow to ensure that the piezoelectric material 123 can fully vibrate. If the first preset length is equal to or slightly less than the second preset length, the solution provided in the embodiment of the present application can also be implemented, but the vibration effect of the piezoelectric material 123 on the cell fluid cavity 16 will be affected.

[0055] It should be noted that, in some embodiments, the cross-sectional shapes of the first electrode 122 , the second electrode 124 and the piezoelectric substrate 123 relative to the sample liquid flow direction are the same as the cross-sectional shape of the cell liquid cavity 1211 relative to the sample liquid flow direction.

[0056] In some embodiments, the liquid collection unit 13 includes: a second air pump, the pipetting device is arranged at the liquid outlet; a waste liquid collection area, the waste liquid collection area is arranged at the outlet of the sample storage unit, and the concentration measurement unit is arranged in the waste liquid collection area; wherein, the control unit is electrically connected to the second air pump, the control unit generates a pipetting control signal and sends it to the second air pump, and the second air pump controls the second air pump according to the pipetting control signal to blow gas toward the outlet of the sample storage unit, moving the sample droplets to the waste liquid collection area.

[0057] In some embodiments, the liquid collecting unit is a well plate, and the concentration measuring unit is a concentration sensor / concentration measuring device disposed in a slot of the well plate, thereby being able to quickly measure the concentration of each slot in the well plate.

[0058] Exemplarily, the acoustic pulse wave is a single pulse signal with a pulse width of 0.5μs to 20μs, a peak-to-peak voltage in the range of 0-500V, and a pulse amplitude of the first pulse amplitude; or, the acoustic pulse wave is a double pulse signal or a bipolar pulse signal with a pulse width of 1μs to 3μs, a pulse interval of 4μs to 30us, and a pulse amplitude of the second pulse amplitude, and the second pulse amplitude is smaller than the first pulse amplitude.

[0059] like Figure 3As shown, an embodiment of the present application provides a single-cell printer 100, which includes the sample mixing device 10 provided by any embodiment of the present application. The single-cell printer 100 can avoid blockage in the microfluidic channel flowing into the sample storage unit due to cell sedimentation, thereby ensuring the normal operation of the cell sorting process and ensuring the efficiency and accuracy of the optical detection results of the sorted sample droplets.

[0060] The serial numbers of the embodiments of the present application are for description only and do not represent the advantages or disadvantages of the embodiments. The above are only specific implementation methods of the present application, but the scope of protection of the present application is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed in this application, and these modifications or replacements should be included in the scope of protection of this application. Therefore, the scope of protection of this application should be based on the scope of protection of the claims.

Claims

1. A sample mixing device, characterized in that: include: A pipeline unit, comprising a liquid input pipeline and a gas input pipeline, wherein the inlet of the liquid input pipeline is used to input the sample liquid, and one side of the liquid input pipeline is connected to the outlet of the gas input pipeline; a mixing unit connected to the inlet of the gas input pipeline; a sample storage unit, wherein the inlet of the sample storage unit is connected to the outlet of the liquid input pipeline, and the sample liquid flows into the cell fluid cavity of the sample storage unit through the inlet of the sample storage unit; a liquid collecting unit, configured to collect sample droplets ejected from the outlet of the sample storage unit; a concentration measuring unit, the concentration measuring unit being disposed in the liquid collecting unit and measuring the concentration of the sample droplets in the liquid collecting unit; a control unit electrically connected to the concentration measurement unit and the mixing unit, the concentration measurement unit sending the concentration of the sample droplet to the control unit, the control unit generating a mixing control signal according to the concentration and sending it to the mixing unit, the mixing unit controlling the gas input pipeline to perform reciprocating gas suction on the sample liquid in the liquid input pipeline according to the mixing control signal, so as to ensure that the sample liquid flowing into the sample storage unit is in a mixed state.

2. The device according to claim 1, characterized in that The mixing unit comprises: A first air pump is connected to the inlet of the gas input pipeline, the control unit is electrically connected to the first air pump, the control unit generates the mixing control signal according to the concentration and sends it to the first air pump, and the first air pump performs reciprocating gas suction on the sample liquid according to the mixing control signal.

3. The device according to claim 2, characterized in that A preset solenoid valve is provided between the first air pump and the gas input pipeline, and the preset solenoid valve is located above the first air pump.

4. The device according to claim 3, characterized in that The control unit is electrically connected to the preset solenoid valve, and the mixing control signal includes an air pump conduction control signal, an air pump shutoff control signal, a solenoid valve conduction control signal, and a solenoid valve shutoff control signal; The control unit sends the air pump conduction control signal to the first air pump and sends the solenoid valve shutoff control signal to the preset solenoid valve; or, The control unit sends the air pump shutoff control signal to the first air pump and sends the solenoid valve conduction control signal to the preset solenoid valve.

5. The device according to claim 1, characterized in that The sample storage unit comprises: a substrate, the substrate comprising a cell fluid cavity, the cell fluid cavity being used to place the sample liquid, the cell fluid cavity comprising a liquid inlet and a liquid outlet, the liquid inlet of the cell fluid cavity being connected to the outlet of the liquid input pipeline; a first electrode, the first electrode being disposed on the substrate and located outside the cell fluid cavity; a piezoelectric substrate, wherein the piezoelectric substrate is made of a piezoelectric material and is disposed on the first electrode; A second electrode: the second electrode is disposed on the piezoelectric substrate, and the polarity of the second electrode is opposite to that of the first electrode; The control unit is connected to the first electrode and the second electrode, and generates a second control signal to the first electrode and the second electrode. The second electrode and the first electrode drive the piezoelectric substrate to vibrate according to the second control signal to propagate an acoustic pulse wave in the sample liquid in the cell fluid cavity, thereby driving the sample liquid to vibrate to form sample droplets, and causing the sample droplets to be ejected through the liquid outlet.

6. The device according to claim 5, characterized in that The cross-sectional shapes of the first electrode, the second electrode, and the piezoelectric substrate relative to the flow direction of the sample liquid are the same as the cross-sectional shape of the cell fluid chamber relative to the flow direction of the sample liquid.

7. The device according to claim 5, characterized in that The length of the first electrode relative to the flow direction of the sample liquid is a preset electrode length, and the length of the piezoelectric material relative to the flow direction of the sample liquid is a preset material length; the preset electrode length is greater than or equal to the preset material length.

8. The device according to claim 5, characterized in that The substrate includes a first substrate and a second substrate. The first electrode is arranged on a first side surface of the first substrate. The second side surface of the first substrate is connected to the first side surface of the second substrate to form the cell fluid cavity.

9. The device according to claim 5, characterized in that The acoustic pulse wave is a single pulse signal with a pulse width of 0.5 μs to 20 μs, a peak-to-peak voltage in the range of 0-500 V, and a pulse amplitude of the first pulse amplitude; or, The acoustic pulse wave is a double pulse signal or a bipolar pulse signal, with a pulse width of 1 μs to 3 μs, a pulse interval of 4 μs to 30 us, and a pulse amplitude of a second pulse amplitude, which is smaller than the first pulse amplitude.

10. A single cell printer, characterized in that The single-cell printer comprises the sample mixing device according to any one of claims 1 to 9.

Images