Sterile sampling device
By using sterilization media to discharge residual samples in the sterile sampling device and sterilize the pipeline, the biosafety risk caused by residual samples in the sampling pipeline is solved, and the safety and environmental protection of the sterile sampling process are achieved.
Patent Information
- Application Number
- CN202422606096.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-28
- Publication Date
- 2025-09-30
- Estimated Expiration
- 2034-10-28
AI Technical Summary
During the sampling process of existing sterile sampling devices, the sample remaining in the sampling pipeline cannot be avoided from being exposed to the environment, resulting in biosafety risks.
A sterile sampling device was designed. After sampling, the residual sample was discharged into the inactivation bottle using sterilization medium, and the pipeline was sterilized to ensure that the pipeline was sterile when exposed to the outside world.
It effectively prevents environmental pollution and human infection and ensures biosafety.
Smart Images
Figure CN223397725U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of aseptic sampling, in particular to an aseptic sampling device. Background Art
[0002] During the cultivation of microorganisms, cells, or viruses, continuous sampling and testing are required to track their growth status and metabolic expression. When it comes to large-scale cultivation of pathogenic microorganisms or viruses, sterile sampling devices that meet biosafety requirements are particularly important. Current sterile sampling devices can ensure the sterility of samples, but when sampling pathogenic microorganisms or viruses, when removing the sampling bottle for testing, it is inevitable that the residual sample in the pipeline will be exposed to the environment, causing biosafety risks and harming people and the environment. Utility Model Content
[0003] The utility model provides a sterile sampling device, which is used to solve the defect of sample residue in the sampling pipeline in the prior art.
[0004] The utility model provides an aseptic sampling device, comprising: a reactor, a first pipeline, a sampling mechanism, a second pipeline, a first valve and an inactivation bottle; the switch valve of the reactor is arranged on the first pipeline, the first pipeline, the sampling mechanism, the second pipeline and the inactivation bottle are connected in sequence, the sampling mechanism is detachably connected to the first pipeline and the second pipeline, and the first valve is arranged on the second pipeline; the sampling mechanism comprises: a third pipeline, a second valve and a sampling bottle, the third pipeline is connected to the first pipeline and the second pipeline, the second valve is arranged on the third pipeline, and the sampling bottle is connected to the third pipeline; wherein, before sampling, the first pipeline is used to pass a sterilization medium, and during sampling, the first pipeline is used for sample flow.
[0005] According to the aseptic sampling device provided by the present invention, the sampling mechanism further includes a three-way valve, and the three ports of the three-way valve are respectively connected to the third pipeline, the second pipeline and the first pipeline.
[0006] According to the aseptic sampling device provided by the present invention, the three-way valve is provided with a rotary handle, and the rotary handle is used to control the conduction of at least two ports among the three ports of the three-way valve.
[0007] According to a sterile sampling device provided by the utility model, the sampling mechanism also includes: a fourth pipeline, a fifth pipeline, a third valve and a fourth valve; the two ends of the fourth pipeline are respectively connected to the first pipeline and the three-way valve, and the two ends of the fifth pipeline are respectively connected to the second pipeline and the three-way valve; the third valve is arranged in the fourth pipeline, and the fourth valve is arranged in the fifth pipeline.
[0008] According to the utility model, a sterile sampling device is provided, which further includes a first quick connector and a second quick connector. The fourth pipeline is connected to the first pipeline through the first quick connector, and the second pipeline is connected to the fifth pipeline through the second quick connector.
[0009] According to a sterile sampling device provided by the present invention, the first quick connector includes: a first connecting part and a second connecting part, the first connecting part and the second connecting part are detachably connected, the first connecting part is connected to the first pipeline, and the second connecting part is connected to the fourth pipeline.
[0010] According to a sterile sampling device provided by the present invention, the second quick connector includes: a third connecting part and a fourth connecting part, the third connecting part and the fourth connecting part are detachably connected, the third connecting part is connected to the second pipeline, and the fourth connecting part is connected to the fifth pipeline.
[0011] According to a sterile sampling device provided by the utility model, the sampling mechanism also includes: an exhaust pipe and a first filter, one end of the exhaust pipe is connected to the air outlet hole on the bottle cap of the sampling bottle, and the other end of the exhaust pipe extends to the outside of the sampling bottle and is connected to the first filter.
[0012] According to the utility model, a sterile sampling device is provided, which further includes a fifth valve and a sixth valve. The fifth valve and the sixth valve are arranged in the first pipeline, and the fifth valve and the sixth valve are located on both sides of the switch valve.
[0013] According to the utility model, a sterile sampling device is provided, which also includes a second filter. The second filter is arranged in the first pipeline, and the second filter is located upstream of the fifth valve.
[0014] The aseptic sampling device provided by the utility model, by arranging the sampling mechanism between the first pipeline and the second pipeline, can use the sterilization medium to discharge the residual sample in the first pipeline again after the sampling mechanism completes sampling, and sterilize the first pipeline at the same time. After the sampling mechanism is removed, when the first pipeline is exposed to the outside, there is no residual sample in it, and no environmental pollution will be caused, thereby ensuring the safety of personnel and the environment. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] In order to more clearly illustrate the technical solutions in the present invention or the prior art, a brief introduction will be given below to the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.
[0016] Figure 1 This is one of the structural diagrams of the sterile sampling device provided by the utility model.
[0017] Figure 2 This is the second structural diagram of the aseptic sampling device provided by the utility model.
[0018] Figure 3 yes Figure 1 Schematic diagram of the structure of the three-way valve shown in FIG.
[0019] Reference numerals:
[0020] 1. Reactor; 2. Sampling mechanism; 4. Inactivation bottle; 5. First valve; 6. Fifth valve; 7. Sixth valve; 8. Second filter;
[0021] 11. On-off valve; 21. Third pipeline; 22. Second valve; 23. Three-way valve; 24. Fourth pipeline; 25. Fifth pipeline; 26. Third valve; 27. Fourth valve; 28. First quick connector; 29. Second quick connector; 31. First pipeline; 32. Second pipeline;
[0022] 211. Sampling bottle; 212. Exhaust pipe; 213. First filter; 231. Rotating handle; 281. First connecting part; 282. Second connecting part. DETAILED DESCRIPTION
[0023] To make the purpose, technical solutions, and advantages of the present invention more clear, the following will be combined with the accompanying drawings to clearly and completely describe the technical solutions of the present invention. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.
[0024] The following combination Figure 1-Figure 3 The invention describes a sterile sampling device.
[0025] like Figure 1 and Figure 2As shown, in an embodiment of the present invention, the aseptic sampling device includes: a reactor 1, a sampling mechanism 2, a first pipeline 31, a second pipeline 32, an inactivation bottle 4 and a first valve 5. The switch valve 11 of the reactor 1 is arranged in the first pipeline 31, the first pipeline 31, the sampling mechanism 2, the second pipeline 32 and the inactivation bottle 4 are connected in sequence, the sampling mechanism 2 is detachably connected to the first pipeline 31 and the second pipeline 32, and the first valve 5 is arranged in the second pipeline 32. The sampling mechanism 2 includes: a third pipeline 21, a second valve 22 and a sampling bottle 211, the third pipeline 21 is connected to the first pipeline 31 and the second pipeline 32, the second valve 22 is arranged in the third pipeline 21, and the sampling bottle 211 is connected to the third pipeline 21. Among them, before sampling, the first pipeline 31 is used to pass the sterilization medium, and during sampling, the first pipeline 31 is used for sample flow.
[0026] Specifically, before sampling, the sampling mechanism 2 is first placed in a sterile breathing bag and sterilized with high-pressure steam. Sodium hydroxide solution is placed in the inactivation bottle 4, and the first valve 5 is closed. Afterwards, after the sampling mechanism 2 has cooled, the sampling mechanism 2 is connected to the first pipeline 31 and the second pipeline 32. The switch valve 11 of the reactor 1 is closed, the second valve 22 is closed, and the first valve 5 is opened. A sterilizing medium is introduced into the first pipeline 31. In an embodiment of the present utility model, the sterilizing medium can be high-pressure steam. The sterilizing medium flows along the first pipeline 31 and the second pipeline 32 and enters the inactivation bottle 4 to sterilize the first pipeline 31, the second pipeline 32 and the first valve 5. At this point, all the pipelines and valves in the entire sterile sampling device are in a sterile state.
[0027] During sampling, the on-off valve 11 of the reactor 1 is opened, the second valve 22 is closed, and the first valve 5 is opened, so that the sample enters the inactivation bottle 4 along the first pipeline 31 and the second pipeline 32 for sewage discharge. Afterwards, the second valve 22 is opened and the first valve 5 is closed. The sample in the reactor 1 enters the sampling bottle 211 along the first pipeline 31 and the third pipeline 21. After the sampling is completed, the on-off valve 11 and the second valve 22 are closed, the first valve 5 is opened, and the sterilization medium is introduced into the first pipeline 31 again. The sterilization medium flows along the first pipeline 31 and the second pipeline 32 into the inactivation bottle 4 to discharge the sample remaining in the first pipeline 31 into the inactivation bottle 4. At the same time, the first pipeline 31 is sterilized to keep the first pipeline 31 in a sterile state.
[0028] After sampling, the second valve 22 is closed and the sampling mechanism 2 is removed for subsequent testing. At this time, there is no sample residue in the first pipeline 31 and the second pipeline 32, and the first pipeline 31 and the second pipeline 32 are in a sterile state. When the first pipeline 31 and the second pipeline 32 are exposed to the outside world, they will not cause environmental pollution to the outside world.
[0029] The sterile sampling device provided by the embodiment of the present invention, by arranging the sampling mechanism between the first pipeline and the second pipeline, can use the sterilization medium to discharge the residual sample in the first pipeline again after the sampling mechanism completes sampling, and sterilize the first pipeline at the same time. After the sampling mechanism is removed, when the first pipeline is exposed to the outside, there is no residual sample in it, and no environmental pollution will be caused, thereby ensuring the safety of personnel and the environment.
[0030] like Figure 1 As shown, in an embodiment of the present invention, the sampling mechanism 2 further includes a three-way valve 23 having three ports, and the first pipeline 31 , the second pipeline 32 and the third pipeline 21 are respectively connected to one port of the three-way valve 23 .
[0031] like Figure 3 As shown, the three-way valve 23 is provided with a rotating handle 231. When the rotating handle 231 is rotated, at least two of the three ports of the three-way valve 23 can be connected, so that the first pipeline 31 is connected to the third pipeline 21; or the first pipeline 31 is connected to the second pipeline 32; or the first pipeline 31, the second pipeline 32 and the third pipeline 21 are all connected.
[0032] like Figure 1 As shown, in an embodiment of the present invention, the sampling mechanism 2 further includes: a fourth pipeline 24, a fifth pipeline 25, a third valve 26, and a fourth valve 27. The fourth pipeline 24 has two ends connected to the first pipeline 31 and the three-way valve 23, respectively; the fifth pipeline 25 has two ends connected to the second pipeline 32 and the three-way valve 23, respectively; the third valve 26 is provided in the fourth pipeline 24, and the fourth valve 27 is provided in the fifth pipeline 25.
[0033] Specifically, when the sampling mechanism 2 is placed in a sterile breathing bag for sterilization, the second valve 22, the third valve 26, and the fourth valve 27 are opened, and the rotary handle 231 of the three-way valve 23 is turned to a state where all three ports are conductive, thereby sterilizing the valves, the third pipeline 21, the fourth pipeline 24, the fifth pipeline 25, and the sampling bottle 211. After sterilization, the third valve 26 and the fourth valve 27 are closed in the biosafety cabinet, and the rotary handle 231 is turned to connect the two ports of the three-way valve 23 connecting to the fourth pipeline 24 and the fifth pipeline 25.
[0034] Before sampling, connect the fourth pipeline 24 to the first pipeline 31, and the fifth pipeline 25 to the second pipeline 32. Close the second valve 22, open the third valve 26, the fourth valve 27, and the first valve 5, and introduce a sterilizing medium into the first pipeline 31. The sterilizing medium then flows along the first pipeline 31, the fourth pipeline 24, the fifth pipeline 25, and the second pipeline 32 into the inactivation bottle 4, thereby sterilizing each pipeline.
[0035] When sampling, open the switch valve 11 and drain the waste first. The sample in the reactor 1 enters the inactivation bottle 4 through the first pipeline 31, the fourth pipeline 24, the fifth pipeline 25 and the second pipeline 32. After that, open the second valve 22, close the fourth valve 27 and the first valve 5, and adjust the three-way valve 23 so that the sample in the reactor 1 flows through the first pipeline 31, the fourth pipeline 24 and the third pipeline 21 into the sampling bottle 211 to complete the sampling.
[0036] After sampling is completed, the on-off valve 11 and the second valve 22 are closed, the fourth valve 27 and the first valve 5 are opened, and a sterilizing medium is introduced into the first pipeline 31. The three-way valve 23 is adjusted to allow the sterilizing medium to enter the inactivation bottle 4 along the first pipeline 31, the fourth pipeline 24, the fifth pipeline 25, and the second pipeline 32. This discharges the sample remaining in the first pipeline 31, the fourth pipeline 24, the fifth pipeline 25, and the second pipeline 32 into the inactivation bottle 4. Simultaneously, the first pipeline 31, the fourth pipeline 24, the fifth pipeline 25, and the second pipeline 32 are sterilized. When the sampling mechanism 2 is removed and the first pipeline 31 and the second pipeline 32 are exposed to the outside world, since there is no sample remaining in the first pipeline 31 and the second pipeline 32 and they are in a sterile state, no environmental pollution is caused.
[0037] like Figure 1 As shown, the sterile sampling device further includes: a first quick connector 28 and a second quick connector 29 . The fourth pipeline 24 is connected to the first pipeline 31 via the first quick connector 28 , and the second pipeline 32 is connected to the fifth pipeline 25 via the second quick connector 29 .
[0038] Specifically, the first quick connector 28 includes a first connecting portion 281 and a second connecting portion 282, which are detachably connected to each other. The first connecting portion 281 is connected to the first pipeline 31, and the second connecting portion 282 is connected to the fourth pipeline 24. The second quick connector 29 includes a third connecting portion and a fourth connecting portion, which are detachably connected to each other. The third connecting portion is connected to the second pipeline 32, and the fourth connecting portion is connected to the fifth pipeline 25.
[0039] Specifically, the first quick connector 28 and the second quick connector 29 are each divided into two parts. Before sampling, when the sampling mechanism 2 is placed in the sterile breathing bag, the second connection portion 282 and the fourth connection portion are also located in the sterile breathing bag, allowing the second connection portion 282 and the fourth connection portion to be sterilized. After sterilization, the second connection portion 282 is connected to the first connection portion 281, and the fourth connection portion is connected to the third connection portion. The third valve 26, the fourth valve 27, and the first valve 5 are opened, and the second valve 22 is closed. A sterilizing medium is introduced into the first pipeline 31. As the sterilizing medium flows along the first pipeline 31, the fourth pipeline 24, the fifth pipeline 25, and the second pipeline 32, the first connection portion 281 of the first quick connector 28 and the third connection portion of the second quick connector 29 are sterilized.
[0040] like Figure 1 As shown, in an embodiment of the present invention, the sampling mechanism 2 also includes: an exhaust pipe 212 and a first filter 213, one end of the exhaust pipe 212 is located in the sampling bottle 211, and the other end of the exhaust pipe 212 extends to the outside of the sampling bottle 211, and the first filter 213 is arranged in the exhaust pipe 212 for filtering the gas flowing through the exhaust pipe 212.
[0041] like Figure 1 and Figure 2 As shown, in an embodiment of the present invention, the sterile sampling device further includes a fifth valve 6 and a sixth valve 7 , which are arranged in the first pipeline 31 , and are located on both sides of the switch valve 11 .
[0042] Specifically, the fifth valve 6 is located upstream of the on-off valve 11 and is used to control the flow of the sterilization medium. The sixth valve 7 is located downstream of the on-off valve 11 and is used to control the flow of the sample or sterilization medium. The sixth valve 7 is closed when not sampling or sterilizing to ensure the sterility of the on-off valve 11 and the reactor 1. Before sampling, when sterilizing the first pipeline 31, both the fifth valve 6 and the sixth valve 7 are opened to sterilize the fifth valve 6 and the sixth valve 7.
[0043] Optionally, in an embodiment of the present invention, the first valve 5 , the second valve 22 , the third valve 26 and the fourth valve 27 may also be Robert clamps.
[0044] exist Figure 1 In the embodiment shown, the sterilization medium may be high-temperature steam. After the sterilization treatment using the high-temperature steam, the medium needs to be cooled before sampling.
[0045] exist Figure 2In the illustrated embodiment, the sterilizing medium can be an alkali solution. In the present embodiment, the aseptic sampling device further includes a second filter 8, which is arranged in the first pipeline 31 and is located upstream of the fifth valve 6. The second filter 8 is used to filter the alkali solution. In the present embodiment, the first pipeline 31 and the second pipeline 32 are sterilized using alkali solution, and then the residual alkali solution in the pipeline is rinsed with sterile water before sampling. After sampling, the residual sample in the first pipeline 31, the fourth pipeline 24, the fifth pipeline 25 and the second pipeline 32 can also be rinsed with alkali solution. At the same time, the first pipeline 31, the fourth pipeline 24, the fifth pipeline 25 and the second pipeline 32 are sterilized so that when the first pipeline 31 and the second pipeline 32 are exposed to the outside after the sampling mechanism 2 is removed, they will not pollute the environment.
[0046] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.
Claims
1. A sterile sampling device, characterized in that: include: A reactor, a first pipeline, a sampling mechanism, a second pipeline, a first valve and an inactivation bottle; The on-off valve of the reactor is arranged on the first pipeline, the first pipeline, the sampling mechanism, the second pipeline and the inactivation bottle are connected in sequence, the sampling mechanism is detachably connected to the first pipeline and the second pipeline, and the first valve is arranged on the second pipeline; The sampling mechanism includes: a third pipeline, a second valve and a sampling bottle, the third pipeline is connected to the first pipeline and the second pipeline, the second valve is arranged on the third pipeline, and the sampling bottle is connected to the third pipeline; Wherein, before sampling, the first pipeline is used to introduce the sterilization medium, and during sampling, the first pipeline is used for the sample to flow.
2. The sterile sampling device according to claim 1, characterized in that: The sampling mechanism further includes a three-way valve, and the three ports of the three-way valve are respectively connected to the third pipeline, the second pipeline and the first pipeline.
3. The sterile sampling device according to claim 2, characterized in that: The three-way valve is provided with a rotary handle, and the rotary handle is used to control the conduction of at least two ports among the three ports of the three-way valve.
4. The sterile sampling device according to claim 2, characterized in that: The sampling mechanism further comprises: a fourth pipeline, a fifth pipeline, a third valve and a fourth valve; Two ends of the fourth pipeline are connected to the first pipeline and the three-way valve respectively, and two ends of the fifth pipeline are connected to the second pipeline and the three-way valve respectively; The third valve is provided in the fourth pipeline, and the fourth valve is provided in the fifth pipeline.
5. The sterile sampling device according to claim 4, characterized in that: The fourth pipeline is connected to the first pipeline through the first quick connector, and the second pipeline is connected to the fifth pipeline through the second quick connector.
6. The sterile sampling device according to claim 5, characterized in that: The first quick connector includes a first connecting portion and a second connecting portion, wherein the first connecting portion and the second connecting portion are detachably connected, the first connecting portion is connected to the first pipeline, and the second connecting portion is connected to the fourth pipeline.
7. The sterile sampling device according to claim 5, characterized in that: The second quick connector includes a third connecting portion and a fourth connecting portion. The third connecting portion is detachably connected to the fourth connecting portion. The third connecting portion is connected to the second pipeline, and the fourth connecting portion is connected to the fifth pipeline.
8. The sterile sampling device according to claim 1, characterized in that: The sampling mechanism further includes: an exhaust pipe and a first filter, one end of the exhaust pipe is communicated with the air outlet on the bottle cap of the sampling bottle, and the other end of the exhaust pipe extends to the outside of the sampling bottle and is connected to the first filter.
9. The sterile sampling device according to claim 1, characterized in that: It also includes a fifth valve and a sixth valve, which are arranged in the first pipeline and are located on both sides of the switch valve.
10. The sterile sampling device according to claim 9, characterized in that: The system further includes a second filter, which is disposed in the first pipeline and is located upstream of the fifth valve.