Plant tissue culture device

Abstract

The utility model belongs to the technical field of plant tissue culture devices, and particularly relates to a plant tissue culture device which comprises a tray, a plurality of plant tissue culture devices and a plurality of plant tissue culture devices, the tray comprises a supporting frame and a hole tray, and the hole tray is placed on the supporting frame and provided with a plurality of circular hole grooves; the tissue culture bag is placed in the hole groove and comprises a bag body, the section of the bag body is circular, the diameter of the bag body is gradually increased from bottom to top, a hard ring is arranged at the top of the bag body, a groove is formed in the bottom of the hard ring, at least three hanging lugs are arranged on the outer wall of the hard ring, and air holes are formed in the bag body; provided is a tissue culture frame. The tissue culture bag is small in bottom area and large in top opening area, and the inoculation area is increased under the condition that the same amount of culture medium is injected; the circular bottom design ensures that the culture medium can be upright without falling after being solidified; the cylindrical bag body is designed, so that the tissue culture bag is not easy to collapse; the tissue culture frame can maintain the shape of the tissue culture bag body not to be deformed, and a liquid culture medium can be conveniently and rapidly canned in production.

Description

Technical Field

[0001] The utility model belongs to the technical field of plant tissue culture devices, and particularly relates to a plant tissue culture device. Background Art

[0002] Plant tissue culture is mainly carried out in specific sterile containers to ensure that the tissue culture plants are protected from microbial contamination. The most common traditional container is a transparent, colorless glass tissue culture bottle. However, glass bottles are expensive, heavy, and inconvenient to carry. They also need to be cleaned before reuse. After being used several times, scratches inevitably appear on the surface of the bottle, resulting in a significant decrease in light transmittance, which directly affects the growth quality of the tissue culture seedlings. Currently, tissue culture bags made of transparent, high-temperature resistant plastic film are easy to fold and store for single use. However, existing tissue culture bags are difficult to support after unfolding, and often require a support structure to be set on the tissue culture bag, which increases the cost of disposable products.

[0003] The Chinese utility model with publication number CN221901814U discloses a plant tissue culture bag, comprising a bag body and a base; the bag body is a square column structure formed by pressing the edges of a transparent plastic film at high temperature, the bottom surface of the bag body is square, the top of the bag body is open, and one side of the opening is connected to a curling sealing strip, and the upper and middle part of one side wall of the bag body is provided with a breathable film, the two side surfaces of the bag body can be folded inward with their respective symmetry axes as the center, at this time, the part where the side surfaces are connected to the bottom surface is folded into an equilateral triangle, and at the same time, the bottom surface is folded outward to fold the bag body into a plane body; the bottom surface of the base is the same size as the bottom surface of the bag body, the height of the base is 1-5 cm, the base is made of transparent plastic material, the top centers of the four sides of the base are respectively connected to the tissue culture rack, the top of the tissue culture rack is connected to a ring rack, the height of the ring rack is lower than the height of the breathable film, but the opening of the tissue culture bag is a quadrilateral, and during inoculation, the bag openings are easily fitted together, which is inconvenient for implanting tissue culture seedlings. Utility Model Content

[0004] The utility model aims to provide a plant tissue culture device to solve the problems in the background art that the square tissue culture bag opening is inconvenient for filling culture medium and inoculation, and the arrangement of tissue culture racks and ring racks in the bag increases manufacturing costs.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A plant tissue culture device, comprising: a tissue culture bag, a tissue culture rack and a tray;

[0007] The tray comprises a supporting frame and a hole plate, wherein the hole plate is placed on the supporting frame and is provided with a plurality of circular hole grooves;

[0008] The tissue culture bag is placed on the tissue culture rack and includes a bag body, the cross-section of the bag body is circular, the diameter of the bag body gradually increases from bottom to top, a hard ring is provided on the top of the bag body, a groove is provided on the bottom of the hard ring, at least three hanging ears are provided on the outer wall of the hard ring, and the bag body is provided with air holes;

[0009] The tissue culture rack is placed in the hole groove and includes an upper ring and a lower ring. The upper ring and the lower ring are connected by a plurality of support bars. The number of the support bars is the same as the number of the hanging ears. The support bars are distributed at equal angles along the upper ring. The first end of the support bar is fixedly connected to the lower ring. The second end of the support bar extends upward at an angle. After being fixedly connected to the upper ring, it is first bent downward and then bent upward to form a hook. The bottom groove of the hard ring is clamped on the upper ring, and the hanging ear is hung on the hook.

[0010] Preferably, the plurality of hole grooves are distributed in a rectangular array.

[0011] Preferably, the sidewall of the hole groove is a slope.

[0012] Preferably, the area of ​​the bottom of the support frame is smaller than the area of ​​the top.

[0013] Preferably, the supporting frame is a rectangular frame with hollow side grooves on the four sides and a hollow bottom groove on the bottom.

[0014] Preferably, the top edge of the support frame is provided with a stepped groove corresponding to the hole plate.

[0015] Preferably, the cross-section of the hard ring is an inverted V-shape.

[0016] Preferably, a bacteria-isolating breathable filter membrane is provided on the vent hole.

[0017] Preferably, the bag body is provided with upper and lower marking ring lines.

[0018] Preferably, the inclination angle of the side wall of the bag body is 1-3°.

[0019] The beneficial effects of the utility model are as follows:

[0020] The tissue culture bag adopts a circular cross-section and a sloped side wall. The bottom area of ​​the tissue culture bag is small and the top opening area is large. When the same amount of culture medium is poured in, the inoculation area is increased. The circular bottom ensures that the culture medium can stand upright after solidification. The cylindrical bag design can prevent the tissue culture bag from collapsing.

[0021] The tissue culture bag is supported by the tissue culture rack, which can maintain the shape of the tissue culture bag without deformation, facilitates the rapid canning of liquid culture medium during production, and improves work efficiency;

[0022] The tray can ensure that the shape of the culture medium at the bottom of the tissue culture bag is maintained when the culture medium cools into a solid;

[0023] The device has a simple structure and low manufacturing cost. The tissue culture bag is a disposable consumable with low manufacturing cost, which saves the cost of cleaning the tissue culture bottle and manpower. The tissue culture rack and tray can be used repeatedly. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The drawings constituting part of this application are provided to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are provided to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0025] Figure 1 This is a three-dimensional structural diagram of a plant tissue culture device according to Example 1 of the present utility model;

[0026] Figure 2 This is a three-dimensional structural diagram of a tissue culture bag of a plant tissue culture device according to Example 1 of the present utility model;

[0027] Figure 3 This is a cross-sectional view of a tissue culture bag of a plant tissue culture device according to Example 1 of the present utility model;

[0028] Figure 4 This is a three-dimensional structural diagram of a tissue culture rack of a plant tissue culture device according to Example 1 of the present utility model;

[0029] Figure 5 This is a three-dimensional structural diagram of a tray of a plant tissue culture device according to Example 1 of the present utility model;

[0030] Figure 6 for Figure 5 A partial enlarged view of point A.

[0031] Among them: 1. tissue culture bag; 11. bag body; 12. air vent; 13. hard ring; 14. hanging ear; 15. groove; 2. tissue culture rack; 21. lower ring; 22. support bar; 23. upper ring; 24. hook; 3. tray; 31. support frame; 32. hole tray; 33. hole groove; 34. side groove; 35. bottom groove; 36. step groove. DETAILED DESCRIPTION

[0032] The present invention will be described in detail below with reference to the accompanying drawings and in combination with embodiments. It should be noted that, unless there is a conflict, the embodiments and features in the embodiments of the present application can be combined with each other.

[0033] The following detailed descriptions are all exemplary descriptions and are intended to provide further details of the present invention. Unless otherwise specified, all technical terms used in this utility model have the same meanings as those commonly understood by those skilled in the art to which this application belongs. The terms used in this utility model are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present utility model.

[0034] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, unless otherwise specified, "multiple" means two or more. In the description of this utility model, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections; direct connections, indirect connections through an intermediate medium, and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on the specific circumstances.

[0035] Example 1

[0036] like Figures 1-6 As shown, a plant tissue culture device includes: a tissue culture bag 1, a tissue culture rack 2 and a tray 3;

[0037] The tissue culture bag 1 is placed on the tissue culture rack 2 and includes a bag body 11. A hard ring 13 is provided around the top of the bag body 11, and a groove 15 is provided at the bottom of the hard ring 13.

[0038] The tissue culture rack 2 is placed in the tray 3 and includes an upper ring 23 and a lower ring 21, wherein the upper ring 23 and the lower ring 21 are connected by a plurality of support bars 22;

[0039] The tray 3 includes a support frame 31 and a hole plate 32. The hole plate 32 is placed on the support frame 31. The hole plate 32 is provided with a plurality of circular hole grooves 33. The tissue culture bag 1 and the tissue culture rack 2 are placed in the hole grooves 33.

[0040] The upper ring 23 cooperates with the groove 15. More specifically, the upper ring 23 is entirely or partially disposed within the annular groove 15, forming a physical limit that can resist vibration displacement to a certain extent.

[0041] In one embodiment, the support bars 22 are evenly distributed at equal angles along the upper ring 23 .

[0042] The first end of the support bar 22 is welded to the lower circular ring 21, and the second end of the support bar 22 extends obliquely upward. After being welded to the upper circular ring 23, it is first bent downward and then bent upward to form a hook 24. The outer wall of the hard ring 13 is provided with at least three hanging ears 14, and the number of the support bars 22 is the same as the number of hanging ears 14. The bottom groove 15 of the hard ring 13 is clamped on the upper circular ring 23, and the hanging ears 14 are hung on the hooks 24, which can further enhance the anti-slip ability of the hard ring 13 on the upper circular ring 23. The hanging ears 14 are evenly distributed along the outer circumference of the hard ring 13, making the suspension force more uniform and preventing the bag body 11 from tilting or rotating and affecting the rapid filling of the culture medium.

[0043] As a preferred example of the above embodiment, the hole plate 32 and the hole groove 33 are integrally formed to eliminate splicing gaps and prevent leakage of culture medium.

[0044] As a preferred example of the above embodiment, the side walls of the hole groove 33 are inclined outward with an inclination angle of 1-3°, which can match the shape of the tissue culture bag 1 and facilitate the stacking of the hole tray 32 for storage and subsequent transportation.

[0045] As a preferred example of the above embodiment, the area of ​​the bottom of the support frame 31 is smaller than the area of ​​the top, so that the support frames 31 can be stacked together to reduce the occupied space.

[0046] As a preferred example of the above embodiment, a stepped groove 36 corresponding to the hole plate 32 is opened on the top edge of the support frame 31, and the edge of the hole plate 32 can be placed in the stepped groove 36 to prevent displacement during operation.

[0047] As a preferred example of the above embodiment, the support frame 31 is a rectangular frame with hollow side grooves 34 on the four sides and a hollow bottom groove 35 on the bottom, which promotes air circulation, prevents mold caused by excessive humidity, and reduces weight.

[0048] As a preferred example of the above embodiment, handles are respectively provided on both sides of the supporting frame 31 to facilitate carrying the supporting frame 31 .

[0049] As a preferred example of the above embodiment, the cross section of the bag body 11 is circular, and the diameter gradually increases from bottom to top.

[0050] As a preferred example of the above embodiment, the bag body 11 is provided with ventilation holes 12 .

[0051] As a preferred example of the above embodiment, the hard ring 13 is made of hard plastic.

[0052] As a preferred example of the above embodiment, the hanging ear 14 is made of a flexible material and can be attached to the edge of the hard ring 13 when folded, so as to facilitate storage.

[0053] As a preferred example of the above embodiment, the bag body 11 is made of a transparent hard plastic film that is resistant to high-pressure sterilization.

[0054] As a preferred example of the above embodiment, there are two air holes 12 , which are arranged on the side wall at a height of 70-100 mm from the bottom, which is beneficial to the flow of air in the tissue culture bag 1 .

[0055] As a preferred example of the above embodiment, a bacteria-isolating breathable filter membrane is provided on the breathable hole 12, and the edge of the breathable filter membrane is fused with the bag body 11 by hot pressing technology.

[0056] As a preferred example of the above embodiment, the bag body 11 is provided with upper and lower identification ring lines, which are 10 mm and 20 mm away from the top of the bag body 11 respectively, to facilitate the subsequent opening and sealing operations of the tissue culture bag 1 during tissue culture inoculation.

[0057] As a preferred example of the above embodiment, the inclination angle of the side wall of the bag body 11 is 1-3°, and the diameter of the bag body 11 decreases from top to bottom, which facilitates the stacking and storage of the tissue culture bags 1.

[0058] As a preferred example of the above embodiment, the plurality of hole slots 33 are distributed in a rectangular array.

[0059] As a specific example of the above embodiment, the height of the bag body 11 is 170 mm, and the diameter of the bottom of the bag body 11 is 60 mm. The dimensions of the tissue culture rack 2 and tray 3 of the device can be adaptively designed according to the dimensions of the bag body 11, and this solution does not impose any specific limitations.

[0060] When preparing the culture medium, place the thawed high-temperature culture medium into tissue culture bag 1. After sealing the bag, place it in a high-pressure steam sterilizer and sterilize it at 121°C for 20-25 minutes. After sterilization, wait for the culture medium to cool into a solid form before inoculating.

[0061] The specific steps are as follows:

[0062] 1. Prepare Tray 3 and Tissue Culture Bag 1

[0063] Place the hole tray 32 in the support frame 31, place the tissue culture rack 2 in the hole groove 33 of the hole tray 32, and place the corresponding number of tissue culture bags 1 in the tissue culture rack 2 to complete the assembly of the device. Hang the opening of the tissue culture bag 1 on the upper ring 23 of the tissue culture rack 2, and hang the ear 14 of the tissue culture bag 1 on the three hooks 24 of the tissue culture rack 2. Arrange the tissue culture bag 1 so that its bottom fits the bottom of the tissue culture rack 2.

[0064] 2. Pour the culture medium into tissue culture bag 1

[0065] Pour the prepared melted liquid culture medium into the tissue culture bag 1, with 50-100 mL of liquid culture medium in each bag. The liquid level of the culture medium in the tissue culture bag 1 is 200 mm. Be careful to avoid the culture medium from contacting the opening above the lower marking ring line at the top of the tissue culture bag 1. If it contacts the opening, wipe off the culture medium with a towel.

[0066] 3. Seal tissue culture bag 1

[0067] Seal the tissue culture bag 1 containing the canned culture medium using a plastic sealer, sealing the upper marking line of the bag 1. After sealing, remove the tissue culture rack 2 and place the bag 1 on the tray 3 for high-pressure steam sterilization. The depth of the cavity 33 is greater than the liquid level of the liquid culture medium in the bag 1.

[0068] 4. High-pressure steam sterilization of culture medium

[0069] The sealed tissue culture bag 1 and the tray 3 are placed in a pressure cooker for sterilization at 121° C. for 20-25 minutes.

[0070] 5. Cooling and solidification of culture medium

[0071] After sterilization, the culture medium is moved to the inoculation room and allowed to cool and solidify naturally.

[0072] 6. Inoculate using tissue culture bag 1

[0073] Open the clean bench and disinfect it in advance, bring the tissue culture bag 1 into the clean bench, and use sterilized scissors to cut off the seal from the logo ring line on the tissue culture bag 1. During inoculation, hold the bottom of the tissue culture bag 1 with your palm, adjust the bag body 11 with your fingers to open the bag mouth, and then use tweezers to access the tissue culture seedlings into the culture medium. After inoculation, seal the lower logo ring line position of the tissue culture bag 1 in the clean bench with a plastic sealing machine. Before sealing, be careful not to flatten the tissue culture bag 1 body and leave as much gas as possible in the tissue culture bag 1.

[0074] 7. Cultivation of tissue culture seedlings in the culture room

[0075] The inoculated tissue culture seedlings are directly placed on the culture rack in the culture room for culture. At this time, the tray 3 is not needed and the tray 3 is used in the next round.

[0076] Throughout this specification, references to terms such as "one embodiment," "example," or "specific example" indicate that a specific feature, structure, material, or characteristic described in conjunction with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of these terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0077] It is understood from common technical knowledge that the present invention may be implemented through other embodiments that do not depart from its spirit or essential features. Therefore, the embodiments disclosed above are, in all respects, merely illustrative and not exclusive. All modifications within the scope of the present invention or equivalent to the scope of the present invention are intended to be encompassed by the present invention.

Claims

1. A plant tissue culture device, characterized in that: include: Tissue culture bag (1), tissue culture rack (2) and tray (3); The tray (3) comprises a supporting frame (31) and a hole plate (32), wherein the hole plate (32) is placed on the supporting frame (31), and a plurality of circular hole grooves (33) are provided on the hole plate (32); A tissue culture bag (1) is placed on a tissue culture rack (2), comprising a bag body (11), wherein the bag body (11) has a circular cross-section, the diameter of the bag body (11) gradually increases from bottom to top, a hard ring (13) is provided on the top of the bag body (11), a groove (15) is provided on the bottom of the hard ring (13), at least three hanging ears (14) are provided on the outer wall of the hard ring (13), and an air vent (12) is provided on the bag body (11); The tissue culture rack (2) is placed in the hole groove (33) and comprises an upper ring (23) and a lower ring (21). The upper ring (23) and the lower ring (21) are connected by a plurality of support bars (22). The number of the support bars (22) is the same as the number of the hanging ears (14). The support bars (22) are distributed at equal angles along the upper ring (23). The first end of the support bar (22) is fixedly connected to the lower ring (21). The second end of the support bar (22) extends upward at an angle and is first bent downward after being fixedly connected to the upper ring (23), and then bent upward to form a hook (24). The bottom groove (15) of the hard ring (13) is clamped on the upper ring (23), and the hanging ear (14) is hung on the hook (24).

2. The plant tissue culture device according to claim 1, wherein: The plurality of hole grooves (33) are distributed in a rectangular array.

3. The plant tissue culture device according to claim 1, wherein: The side wall of the hole groove (33) is an inclined surface.

4. The plant tissue culture device according to claim 1, wherein: The area of ​​the bottom of the support frame (31) is smaller than the area of ​​the top.

5. The plant tissue culture device according to claim 1, wherein: The supporting frame (31) is a rectangular frame with hollow side grooves (34) on four sides and a hollow bottom groove (35) on the bottom.

6. The plant tissue culture device according to claim 1, wherein: The top edge of the support frame (31) is provided with a stepped groove (36) corresponding to the hole plate (32).

7. The plant tissue culture device according to claim 1, wherein: The cross section of the hard ring (13) is an inverted V shape.

8. The plant tissue culture device according to claim 1, wherein: The ventilation hole (12) is provided with a bacteria-isolating ventilation filter membrane.

9. The plant tissue culture device according to claim 1, wherein: The bag body (11) is provided with upper and lower marking ring lines.

10. The plant tissue culture device according to claim 1, wherein: The inclination angle of the side wall of the bag body (11) is 1-3°.

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