Stem cell extracting and filtering device

Abstract

The utility model relates to the technical field of stem cell extraction, in particular to a stem cell extracting and filtering device which comprises a box cover hinged to the top of a centrifugal machine, and a centrifugal filtering mechanism for extracting and filtering stem cells through a density gradient centrifugation method is arranged in the centrifugal machine. An extraction mechanism for performing targeted extraction on the layered stem cells is arranged in the centrifugal filtration mechanism. The centrifugal motor drives the placement disc to rotate so as to drive the test tube to rotate, so that a stem cell mixed solution in the test tube is centrifugally separated, and compared with a traditional filter screen filtering mode, the centrifugal separation method can accurately separate out a stem cell layer, and after the stem cell mixed solution in the test tube is centrifugally separated out and layered, the stem cell layer can be separated out. According to the centrifugal separation position of the stem cells, the extraction needle cylinder is inserted into the discharging pipe, and then the sealing valve is opened, so that the stem cells are pertinently filtered and extracted, and the stem cells are prevented from being polluted by other impurities during extraction.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell extraction, in particular to a stem cell extraction filtering device. Background Art

[0002] For example, Chinese patent publication number CN212596437U discloses a filtration separation device for stem cell extraction. A motor, via an output shaft, drives a mounting disc in a separation chamber, which in turn rotates test tubes on the disc. When the disc rotates at high speed, if the density of the suspended particles is greater than that of the surrounding medium, the particles move away from the axis and settle. If the density of the particles is lower than that of the surrounding medium, the particles move toward the axis and float, separating the stem cells in the test tubes.

[0003] However, after the above-mentioned device uses density gradient centrifugation to separate the stem cells inside the test tube into layers, when the stem cells are poured out, the stem cells will inevitably be mixed with materials from other layers. Even if needle aspiration is used, a certain amount of materials from other layers will be mixed in through the needle during the process of puncturing the pillow into the stem cell layer, affecting the purity of the stem cells. Utility Model Content

[0004] In response to the deficiencies of the prior art, the present invention provides a stem cell extraction and filtration device, which solves the technical problem in the prior art that other substances may be mixed into the separated stem cells during extraction, thereby affecting the purity of the stem cells.

[0005] To solve the above technical problems, the present invention provides the following technical solution: a stem cell extraction and filtration device, comprising a cover hingedly connected to the top of a centrifuge, wherein the centrifuge is internally provided with a centrifugal filtration mechanism for extracting and filtering stem cells using density gradient centrifugation, and an extraction mechanism is provided within the centrifugal filtration mechanism for specifically extracting the stratified stem cells.

[0006] The extraction mechanism includes a test tube installed in a centrifugal filter mechanism, a plurality of discharge pipes are vertically and equidistantly installed on the test tube, a sealing valve is installed on the discharge pipe, and a threaded cover is threadedly connected to the top of the test tube.

[0007] Preferably, the centrifugal filtration mechanism includes a placement plate installed in the centrifuge, a connecting rod is installed at the top center of the placement plate, a limit plate is installed on the top of the connecting rod, and through holes are opened in a circular shape and equidistantly on the limit plate, a rotating shaft is installed at the bottom center of the placement plate, a centrifugal motor for driving the rotating shaft to rotate is installed at the bottom of the centrifuge, and a clamping assembly for clamping and fixing test tubes of different diameters is provided inside the through hole.

[0008] Preferably, the clamping assembly includes an electromagnetic ring installed on the inner wall of the through hole, a sliding sleeve is installed on the inner wall of the electromagnetic ring in a ring-shaped and equidistant manner, and a permanent magnetic slider is connected to the inside of the sliding sleeve for sliding left and right. A sliding rod is installed on the permanent magnetic slider and is connected to the sliding hole opened on the sliding sleeve for sliding left and right, and a clamping plate is installed on the outer end of the sliding rod.

[0009] Preferably, the placement plate is provided with a plug-in slot vertically corresponding to the through hole on the limiting plate, and magnets are installed inside the plug-in slot and at the bottom of the test tube.

[0010] Preferably, an observation window made of transparent tempered glass is installed on the front of the centrifuge, and rubber suction cups are installed at the four corners of the bottom of the centrifuge.

[0011] Preferably, a controller is installed on the right side of the front of the centrifuge, and the centrifugal filtering mechanism is electrically connected to the controller.

[0012] By means of the above technical solution, the present invention provides a stem cell extraction and filtration device, which has at least the following beneficial effects:

[0013] 1. The utility model uses a centrifugal motor to drive the placement plate to rotate, thereby driving the test tube to rotate, thereby centrifuging the stem cell mixed solution inside the test tube. Compared with the traditional filter mesh filtration method, this centrifugal separation method can accurately separate and precipitate the stem cell layer.

[0014] 2. The utility model energizes the electromagnetic ring to push the permanent magnetic slider to move outward along the inner wall of the sliding sleeve, and pushes the sliding rod and the clamping plate at its outer end to move outward to clamp and fix test tubes of different diameters, so that they will not be thrown away during the centrifugal separation process.

[0015] 3. In the present invention, after the stem cell mixed solution in the test tube is separated and stratified by centrifugation, the extraction syringe is inserted into the discharge tube according to the position where the stem cells are centrifuged, and then the sealing valve is opened to filter and extract the stem cells in a targeted manner, thereby preventing the stem cells from being contaminated by other impurities during extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The drawings described herein are used to provide further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute improper limitations on the present application.

[0017] In the attached figure:

[0018] Figure 1 This is a schematic diagram of the overall structure of the utility model;

[0019] Figure 2 This is a schematic diagram of the independent structure of the centrifugal filter mechanism of the utility model;

[0020] Figure 3 It is partial section bird's-eye view structure schematic diagram of the clamping assembly of the utility model;

[0021] Figure 4 It is split schematic diagram of the structure test tube and the placing disc of the utility model;

[0022] Figure 5 It is partial independent amplification structure schematic diagram of the extraction mechanism of the utility model.

[0023] In the drawing: 1, centrifuge; 2, box cover; 3, centrifugal filtering mechanism; 301, placing disc; 302, connecting rod; 303, limiting disc; 304, rotating shaft; 305, centrifugal motor; 306, clamping assembly; 3061, electromagnetic ring; 3062, sliding sleeve; 3063, permanent magnet sliding block; 3064, sliding rod; 3065, clamping plate; 307, plug-in slot; 308, magnet; 4, extraction mechanism; 401, test tube; 402, discharge pipe; 403, sealing valve; 404, screw cap; 5, observation window; 6, rubber suction cup; 7, controller. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0025] Embodiment 1

[0026] Based on the problem that the existing prior art causes other substances to mix in when extracting the separated stem cells, affecting the purity of the stem cells, the embodiment provides a stem cell extraction filtering device, please refer to Figure 1-Figure 5 The embodiment provides a stem cell extraction filtering device, which can accurately extract the stem cells filtered by centrifugation. The stem cell extraction filtering device comprises a box cover 2 hinged to the top of a centrifuge 1, the inside of the centrifuge 1 is provided with a centrifugal filtering mechanism 3 for extracting and filtering the stem cells by using the density gradient centrifugation method, the centrifugal filtering mechanism 3 is provided with an extraction mechanism 4 for targeted extraction of the layered stem cells, the stem cells in the stem cell mixed solution in the test tube 401 are separated and filtered by the centrifugal filtering mechanism 3, and the separated stem cells can be accurately extracted by the discharge pipe 402, so that the stem cells are prevented from being contaminated.

[0027] Because existing technologies may cause other substances to mix in when extracting separated stem cells, affecting the purity of the stem cells, the device is provided with an extraction mechanism 4, which includes a test tube 401 installed in the centrifugal filtration mechanism 3. Multiple discharge pipes 402 are vertically and equidistantly installed on the test tube 401, and a sealing valve 403 is installed on the discharge pipe 402. The top of the test tube 401 is threadedly connected with a threaded cap 404. After the stem cell mixed solution in the test tube 401 is centrifuged and separated into layers, the extraction syringe is inserted into the discharge pipe 402 according to the position where the stem cells are centrifuged, and then the sealing valve 403 is opened to filter and extract the stem cells in a targeted manner, thereby preventing the stem cells from being contaminated by other impurities during extraction.

[0028] Since conventional extraction methods often use a filter to extract stem cells, but in this process, stem cells will inevitably be mixed with other substances, and secondary screening is required later, the device is also provided with a centrifugal filtration mechanism 3, which includes a placement plate 301 installed in the centrifuge 1, a connecting rod 302 is installed at the top center of the placement plate 301, a limiting plate 303 is installed on the top of the connecting rod 302, and a through hole is opened in an annular shape and equidistantly on the limiting plate 303, a rotating shaft 304 is installed at the bottom center of the placement plate 301, and the centrifuge 1 is provided with a centrifugal filtration mechanism 3. A centrifugal motor 305 for driving the rotating shaft 304 to rotate is installed at the bottom of the heart machine 1. A clamping assembly 306 for clamping and fixing test tubes 401 of different diameters is provided inside the through hole. The test tube 401 is inserted into the through hole on the limiting plate 303, and then the centrifugal motor 305 is started to drive the rotating shaft 304 to rotate, and then drive the placement plate 301 to rotate, thereby driving the test tube 401 to rotate, thereby centrifuging the stem cell mixed solution inside the test tube 401. Compared with the traditional filter mesh filtration method, this centrifugal separation method can accurately separate and precipitate the stem cell layer.

[0029] In order to further improve the fixing effect of the test tube 401, the device is provided with a plug-in slot 307 on the placement plate 301, which is vertically corresponding to the through hole on the limit plate 303. Magnets 308 are installed inside the plug-in slot 307 and at the bottom of the test tube 401. When the test tube 401 is inserted into the plug-in slot 307 opened on the placement plate 301, the magnet 308 at the bottom of the test tube 401 and the magnet 308 inside the plug-in slot 307 attract each other, thereby further improving the placement stability of the test tube 401.

[0030] An observation window 5 made of transparent tempered glass is installed on the front of the centrifuge 1. Rubber suction cups 6 are installed at the four corners of the bottom of the centrifuge 1. The rubber suction cups 6 are attached to the table, so that the centrifuge 1 can be stably placed. The provision of the observation window 5 facilitates real-time observation of the separation and precipitation of stem cells in the test tube 401.

[0031] A controller 7 is installed on the right side of the front of the centrifuge 1. The centrifugal filtering mechanism 3 is electrically connected to the controller 7, and the operation of the device is controlled by the controller 7.

[0032] Example 2

[0033] On the basis of Example 1, Figure 1-Figure 5 As shown, in the process of stem cell extraction, it is often necessary to compare different doses of stem cell mixed solutions in the experimental stage to obtain the best ratio. In this process, test tubes 401 of different capacities are often used for experiments. Therefore, it is necessary to clamp and fix the test tubes 401 of different capacities for centrifugal separation. Therefore, the device is further provided with a clamping assembly 306, which includes an electromagnetic ring 3061 installed on the inner wall of the through hole. Slide sleeves 3062 are equidistantly installed on the inner wall of the electromagnetic ring 3061 in an annular shape, and the inner part of the slide sleeve 3062 is connected to a permanent The magnetic slider 3063 is provided with a slide rod 3064 which slides left and right and is connected to the slide hole opened on the sleeve 3062. The outer end of the slide rod 3064 is provided with a clamping plate 3065. When the test tube 401 is inserted into the through hole, the electromagnetic ring 3061 is energized, thereby generating a repulsive force between the electromagnetic ring 3061 and the permanent magnetic slider 3063, pushing the permanent magnetic slider 3063 to move outward along the inner wall of the sleeve 3062, and pushing the slide rod 3064 and the clamping plate 3065 at its outer end to move outward to clamp and fix the test tubes 401 of different diameters, so that they will not be thrown away during the centrifugal separation process.

[0034] It should be noted that, in this article, the terms "comprises", "includes" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or apparatus that includes a series of elements includes not only those elements, but also includes other elements not explicitly listed, or also includes elements that are inherent to such process, method, article or apparatus.

[0035] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A stem cell extraction and filtration device, comprising a cover (2) hingedly connected to the top of a centrifuge (1), characterized in that: The centrifuge (1) is provided with a centrifugal filtration mechanism (3) for extracting and filtering stem cells using a density gradient centrifugation method, and the centrifugal filtration mechanism (3) is provided with an extraction mechanism (4) for specifically extracting the stratified stem cells; The extraction mechanism (4) comprises a test tube (401) installed in the centrifugal filtering mechanism (3), a plurality of discharge pipes (402) are vertically and equidistantly installed on the test tube (401), a sealing valve (403) is installed on the discharge pipe (402), and a threaded cover (404) is threadedly connected to the top of the test tube (401).

2. The stem cell extraction and filtration device according to claim 1, characterized in that: The centrifugal filtering mechanism (3) comprises a placement plate (301) installed in a centrifuge (1), a connecting rod (302) is installed at the top center of the placement plate (301), a limiting plate (303) is installed at the top of the connecting rod (302), and the limiting plate (303) is provided with through holes equidistantly and annularly formed through the limiting plate (303), a rotating shaft (304) is installed at the bottom center of the placement plate (301), a centrifugal motor (305) for driving the rotating shaft (304) to rotate is installed at the bottom of the centrifuge (1), and a clamping assembly (306) for clamping and fixing test tubes (401) of different diameters is provided inside the through hole.

3. The stem cell extraction and filtration device according to claim 2, characterized in that: The clamping assembly (306) includes an electromagnetic ring (3061) installed on the inner wall of the through hole, a sliding sleeve (3062) is installed on the inner wall of the electromagnetic ring (3061) in an annular shape and equidistantly, and a permanent magnetic slider (3063) is connected to the inside of the sliding sleeve (3062) for sliding left and right. The permanent magnetic slider (3063) is installed with a sliding rod (3064) that is connected to the sliding hole opened in the sliding sleeve (3062) for sliding left and right, and a clamping plate (3065) is installed on the outer end of the sliding rod (3064).

4. The stem cell extraction and filtration device according to claim 2, characterized in that: The placement plate (301) is provided with a plug-in slot (307) vertically corresponding to the through hole on the limiting plate (303), and magnets (308) are installed inside the plug-in slot (307) and at the bottom of the test tube (401).

5. The stem cell extraction and filtration device according to claim 1, characterized in that: An observation window (5) made of transparent tempered glass is installed on the front of the centrifuge (1), and rubber suction cups (6) are installed at the four corners of the bottom of the centrifuge (1).

6. The stem cell extraction and filtration device according to claim 1, characterized in that: A controller (7) is installed on the right side of the front of the centrifuge (1), and the centrifugal filtering mechanism (3) is electrically connected to the controller (7).

Images