Cell lysis buffer separator

Abstract

The utility model discloses a cell lysis buffer separator and relates to the technical field of separation. The cell lysis buffer separator comprises a treatment barrel, a cover plate and a driving motor, the cover plate is arranged on the treatment barrel, a separation barrel is arranged on the cover plate, and the separation barrel is located in the treatment barrel. According to the cell lysis solution separator, the cell lysis solution can be separated, and after the treatment barrel and the cover plate are separated, the separation barrel can be conveniently taken out from the interior of the treatment barrel, so that when more impurities are adhered to the surface of the separation barrel, a worker can conveniently wash the surface of the separation barrel to remove the impurities on the surface of the separation barrel; the situation that the follow-up centrifugal effect on the cell lysis solution is affected by many impurities on the separation barrel is avoided, the cleaning mode is simple, operation of workers is facilitated, the cleaning efficiency of the workers on the separation barrel is improved, after the treatment barrel and the cover plate are separated, the workers can conveniently clean the interior of the treatment barrel, and the cleaning efficiency is improved. And the cleaning work efficiency of workers is further improved.

Description

Technical Field

[0001] The utility model relates to the technical field of separation, in particular to a cell lysis solution separator. Background Art

[0002] Cell lysate is a rapid cell tissue lysis solution that can be used to extract proteins. In industry, the volume of cell lysate processed is often very large. The large amount of cell fragments produced after bacterial cell disruption, as well as the released nucleic acids and protein precipitation, make the lysate components very complex and viscous. Pre-treatment of the lysate before centrifugation usually uses filtration separation to clarify the lysate.

[0003] Currently, the centrifuge cylinders of some cell lysis solution separation devices are mostly fixed inside the centrifuge equipment. After long-term centrifugation, a large amount of impurities will adhere to the surface of the centrifuge cylinder, making it inconvenient for workers to clean it. Utility Model Content

[0004] The purpose of the utility model is to provide a cell lysis solution separator, which can solve the problem that most centrifuge cylinders are fixed inside the centrifugal equipment. After long-term centrifugation, a large amount of impurities will adhere to the surface of the centrifuge cylinder, making it inconvenient for workers to clean it.

[0005] To achieve the above-mentioned object, the present invention provides the following technical solutions: a cell lysate separator, comprising a processing barrel, a cover plate and a driving motor;

[0006] The cover plate is arranged on the processing barrel, and a separation cylinder is arranged on the cover plate, and the separation cylinder is located inside the processing barrel;

[0007] The driving motor is used to drive the separation drum to rotate.

[0008] Preferably, a rotating bottom rod is rotatably mounted on the inner bottom of the processing barrel, and a supporting chassis is fixedly mounted on the top of the rotating bottom rod.

[0009] Preferably, a cover plate is placed on the top of the processing barrel, and a hollow rotating column is rotatably installed on the bottom of the cover plate. One end of the hollow rotating column extends to the top of the cover plate and is rotatably installed with a connecting box. A feed pipe is fixedly installed on the top of the connecting box, and support rods are fixedly installed on both sides of the connecting box. One end of the support rod is fixedly installed on the top of the cover plate, and a separation cylinder is fixedly installed on the bottom of the hollow rotating column, and one end of the hollow rotating column extends to the interior of the separation cylinder.

[0010] Preferably, a driving motor is fixedly mounted on the top of the cover plate, a rotating shaft is fixedly mounted on the rotating shaft of the driving motor, gears are fixedly mounted on the outer wall of the rotating shaft and the outer wall of the hollow rotating column, and there are two groups of gears that are meshed with each other.

[0011] Preferably, a limiting ring is fixedly mounted on the outer wall of the separation cylinder, and an opening for the limiting ring to move is provided on the support chassis, so that the support chassis can be driven to rotate when the separation cylinder rotates.

[0012] Preferably, a limiting block is fixedly installed on the top of the processing barrel, and an opening for the limiting block to move is opened on the cover plate.

[0013] Preferably, a discharge pipe is fixedly mounted on the outer wall of the separation cylinder, one end of the discharge pipe extends into the interior of the separation cylinder and a stop valve is provided on the discharge pipe.

[0014] Compared with the prior art, the beneficial effects of the present invention are: the cell lysis liquid separator can be separated by the setting of the processing barrel and the cover plate. After the processing barrel and the cover plate are separated, it is convenient to take out the separation cylinder from the inside of the processing barrel. In this way, when there are more impurities adhering to the surface of the separation cylinder, it is convenient for workers to rinse the surface of the separation cylinder to remove the impurities on the surface of the separation cylinder, avoiding the impurities on the separation cylinder affecting the subsequent centrifugation effect of the cell lysis liquid. In addition, this cleaning method is relatively simple, convenient for workers to operate, and improves the workers' cleaning efficiency of the separation cylinder. After the processing barrel and the cover plate are separated, it is also convenient for workers to clean the inside of the processing barrel, further improving the efficiency of the workers' cleaning work. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The present invention will be further described below with reference to the accompanying drawings and embodiments:

[0016] Figure 1 It is a three-dimensional diagram of the utility model;

[0017] Figure 2 It is a structural diagram of the utility model;

[0018] Figure 3 It is an enlarged view of part A of the present utility model.

[0019] Figure numerals: 1. Processing barrel; 2. Rotating bottom rod; 3. Support chassis; 4. Limit block; 5. Cover plate; 6. Hollow rotating column; 7. Separation cylinder; 8. Limit ring; 9. Driving motor; 10. Rotating shaft; 11. Gear; 12. Connecting box; 13. Feed pipe. DETAILED DESCRIPTION

[0020] This section will describe in detail the specific embodiments of the present invention. The preferred embodiments of the present invention are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and overall technical solution of the present invention, but it cannot be understood as a limitation on the scope of protection of the present invention.

[0021] See also Figure 1-3The utility model provides a technical solution: a cell lysis liquid separator, comprising a processing barrel 1, a cover plate 5 and a driving motor 9; the cover plate 5 is arranged on the processing barrel 1, and a separation cylinder 7 is provided on the cover plate 5, and the separation cylinder 7 is located inside the processing barrel 1; the driving motor 9 is used to drive the separation cylinder 7 to rotate, and a rotating bottom rod 2 is rotatably installed on the bottom inner side of the processing barrel 1, and a supporting chassis 3 is fixedly installed on the top of the rotating bottom rod 2.

[0022] Furthermore, a cover plate 5 is placed on the top of the processing barrel 1, and a hollow rotating column 6 is rotatably installed on the bottom of the cover plate 5. One end of the hollow rotating column 6 extends to the top of the cover plate 5 and is rotatably installed with a connecting box 12. A feed pipe 13 is fixedly installed on the top of the connecting box 12. Support rods are fixedly installed on both sides of the connecting box 12. One end of the support rod is fixedly installed on the top of the cover plate 5. A separation cylinder 7 is fixedly installed on the bottom of the hollow rotating column 6. One end of the hollow rotating column 6 extends to the inside of the separation cylinder 7. This arrangement can separate them. After the processing barrel 1 and the cover plate 5 are separated , it is convenient to take out the separation cylinder 7 from the inside of the processing barrel 1. In this way, when there are more impurities adhering to the surface of the separation cylinder 7, it is convenient for workers to rinse the surface of the separation cylinder 7 to remove the impurities on the surface of the separation cylinder 7, so as to avoid the impurities on the separation cylinder 7 affecting the subsequent centrifugation effect of the cell lysate. In addition, this cleaning method is relatively simple and convenient for workers to operate, thereby improving the workers' cleaning efficiency of the separation cylinder 7. After the processing barrel 1 and the cover plate 5 are separated, it is also convenient for workers to clean the inside of the processing barrel 1, further improving the efficiency of the workers' cleaning work.

[0023] Furthermore, a driving motor 9 is fixedly installed on the top of the cover plate 5, and a rotating shaft 10 is fixedly installed on the rotating shaft of the driving motor 9. Gears 11 are fixedly installed on the outer wall of the rotating shaft 10 and the outer wall of the hollow rotating column 6. There are two groups of gears 11, and the two groups of gears 11 are meshed with each other.

[0024] Secondly, a limit ring 8 is fixedly installed on the outer wall of the separation cylinder 7, an opening for the limit ring 8 to move is opened on the supporting chassis 3, a limit block 4 is fixedly installed on the top of the processing barrel 1, and an opening for the limit block 4 to move is opened on the cover plate 5. A discharge pipe is fixedly installed on the outer wall of the separation cylinder 7, one end of the discharge pipe extends to the interior of the separation cylinder 7 and a stop valve is provided on the discharge.

[0025] Working principle: When in use, the cell lysate is added through the feed pipe 13, and then the cell lysate is transported to the inside of the separation cylinder 7 through the connecting box 12 and the hollow rotating column 6. After the control drive motor 9 is started, the drive motor 9 starts to drive the rotating shaft 10 to rotate. The rotation of the rotating shaft 10 drives the hollow rotating column 6 to rotate through two sets of gears 11. The rotation of the hollow rotating column 6 can drive the separation cylinder 7 to rotate. The rotation of the separation cylinder 7 can separate the cell lysate inside it. After separation, the cover plate 5 is lifted by the traction equipment to separate the processing barrel 1 from the cover plate 5, and then the separated cell lysate can be taken out through the discharge pipe on the separation cylinder 7. When there are more impurities adhering to the surface of the separation cylinder 7, the processing barrel 1 and the cover plate 5 are separated and the surface of the separation cylinder 7 can be cleaned.

[0026] The embodiments of the present invention are described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Various changes can be made within the scope of knowledge possessed by ordinary technicians in the technical field without departing from the purpose of the present invention.

Claims

1. A cell lysate separator, characterized in that: include: Processing bucket (1); A cover plate (5), the cover plate (5) is arranged on the processing barrel (1), a separation barrel (7) is arranged on the cover plate (5), and the separation barrel (7) is located inside the processing barrel (1); A driving motor (9) is used to drive the separation cylinder (7) to rotate.

2. A cell lysate separator according to claim 1, characterized in that: A rotating bottom rod (2) is rotatably mounted on the inner bottom of the processing barrel (1), and a supporting chassis (3) is fixedly mounted on the top of the rotating bottom rod (2).

3. A cell lysate separator according to claim 2, characterized in that: A cover plate (5) is placed on the top of the processing barrel (1), a hollow rotating column (6) is rotatably mounted on the bottom of the cover plate (5), one end of the hollow rotating column (6) extends to the top of the cover plate (5) and is rotatably mounted on a connecting box (12), a feeding pipe (13) is fixedly mounted on the top of the connecting box (12), support rods are fixedly mounted on both sides of the connecting box (12), one end of the support rods is fixedly mounted on the top of the cover plate (5), a separation cylinder (7) is fixedly mounted on the bottom of the hollow rotating column (6), and one end of the hollow rotating column (6) extends to the interior of the separation cylinder (7).

4. A cell lysate separator according to claim 3, characterized in that: A driving motor (9) is fixedly mounted on the top of the cover plate (5); a rotating shaft (10) is fixedly mounted on the rotating shaft of the driving motor (9); gears (11) are fixedly mounted on the outer wall of the rotating shaft (10) and the outer wall of the hollow rotating column (6); the gears (11) are in two groups, and the two groups of gears (11) are meshed with each other.

5. A cell lysate separator according to claim 4, characterized in that: A limiting ring (8) is fixedly mounted on the outer wall of the separation cylinder (7), and an opening for the limiting ring (8) to move is provided on the supporting chassis (3).

6. A cell lysate separator according to claim 5, characterized in that: A limit block (4) is fixedly mounted on the top of the processing barrel (1), and an opening for the limit block (4) to move is provided on the cover plate (5).

7. A cell lysate separator according to claim 6, characterized in that: A discharge pipe is fixedly mounted on the outer wall of the separation cylinder (7), one end of the discharge pipe extends into the interior of the separation cylinder (7) and a stop valve is provided on the discharge pipe.

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