Tetracarboxylic acid bacteria culture tank device

Abstract

The utility model discloses a carboxylic acid bacteria culture tank device which comprises a fermentation and propagation tank body, and a culture solution stirring mechanism is assembled and connected on the fermentation and propagation tank body; the culture solution stirring mechanism comprises a stirring motor mounted above the fermentation and propagation tank body, the stirring motor is provided with a stirring material shaft, and the stirring material shaft is provided with a cylindrical stirring pipe structure; the cylindrical stirring pipe structure comprises a plurality of cylindrical stirring pipes with cylindrical structures, annular pipes are mounted at the tops and the bottoms of the cylindrical stirring pipes respectively, and the inner side walls of the annular pipes are fixedly mounted on the stirring shaft through connecting pipes; the carboxylic acid bacteria culture tank device further comprises an airflow stirring structure arranged on the culture solution stirring mechanism, and in the process that the cylindrical stirring pipe rotationally stirs the culture solution, the airflow stirring structure pumps nitrogen into the fermentation and propagation tank body for airflow stirring. According to the device, the dispersion degree of nutrient substances and expanded culture microorganisms is greatly improved, so that the expanding culture yield of the microorganisms is increased.

Description

Technical Field

[0001] The utility model belongs to the technical field of butyric acid bacteria culture, in particular to a butyric acid bacteria culture tank device. Background Art

[0002] Butyric acid bacteria are anaerobic bacteria. Butyric acid bacteria and its metabolite, butyric acid, can effectively improve the microecological environment within the animal intestine and enhance intestinal function. Therefore, Butyric acid bacteria have a wide range of industrial applications, including improving the intestinal microecology in fish farming processes.

[0003] The industrial method for producing butyric acid bacteria is to use fermentation and expansion. Specifically, a certain amount of butyric acid bacteria is added to a fermentation tank, and the butyric acid bacteria are multiplied in large quantities within the fermentation tank to obtain butyric acid bacteria through fermentation and expansion. During the fermentation and expansion process, the nutrient solution needs to be stirred. The stirring method is used to fully disperse the butyric acid bacteria obtained in large quantities in the nutrient solution, which increases the distribution space of the butyric acid bacteria and increases the butyric acid bacteria's utilization of nutrients.

[0004] However, in actual working process, the stirring device used on the existing tank body is a stirring device with a stirring impeller or a stirring blade. During the rotation process, the stirring structure has a large shear force, and the bacteria are a cell wall structure. Therefore, after stirring, the cell walls of a large number of densely multiplied microorganisms are broken, which leads to the death of a large number of cultured microorganisms, thereby seriously affecting the number of microbial bacteria obtained and resulting in too low expansion yield. Utility Model Content

[0005] Based on the above background, the purpose of the present invention is to provide a butyric acid bacteria culture tank device.

[0006] In order to achieve the above objectives, the present invention adopts the following technical solutions:

[0007] A butyric acid bacteria culture tank device comprises a fermentation and expansion tank body, wherein the fermentation and expansion tank body is equipped with a culture liquid stirring mechanism, and during the stirring process of the culture liquid stirring mechanism, the breaking effect of the stirring structure on the bacterial cell wall is reduced;

[0008] The culture liquid stirring mechanism includes a stirring motor installed above the fermentation and expansion tank body, the stirring motor is equipped with a stirring shaft, and the stirring shaft is equipped with a cylindrical stirring tube structure;

[0009] The cylindrical stirring tube structure includes a plurality of cylindrical stirring tubes, the top and bottom of each cylindrical stirring tube are respectively provided with an annular tube, and the inner side wall of each annular tube is fixedly mounted on the stirring shaft via a connecting tube;

[0010] The butyric acid bacteria culture tank device also includes an airflow stirring structure provided on the culture liquid stirring mechanism. When the cylindrical stirring tube rotates to stir the culture liquid, the airflow stirring structure pumps nitrogen into the fermentation and expansion tank body to perform airflow stirring.

[0011] Preferably, the top and bottom of the cylindrical stirring tube are respectively fixedly assembled with an outer annular tube and an inner annular tube concentrically arranged with the outer annular tube;

[0012] A plurality of cylindrical stirring tubes distributed around the circumference are fixedly installed between the outer annular tubes and the inner annular tubes.

[0013] Preferably, the airflow stirring structure includes a plurality of nitrogen exhaust holes opened on the cylindrical stirring tube;

[0014] The cylindrical stirring tube is connected to the corresponding outer annular tube and the inner annular tube;

[0015] The outer annular tube and the inner annular tube are connected to the stirring shaft. The upper end of the stirring shaft is connected to a nitrogen inlet pipe, and the nitrogen inlet pipe is connected to an external nitrogen source.

[0016] Preferably, the top and bottom of the cylindrical stirring tube are respectively integrally formed with threaded connecting tubes, and the threaded connecting tubes are threadedly connected to the corresponding outer annular tube and inner annular tube;

[0017] The threaded connecting pipe is provided with a first air hole, which is connected to the lumens of the outer annular pipe and the inner annular pipe.

[0018] Preferably, the threaded connecting pipe is threadedly connected with a nut that is locked on the corresponding outer annular pipe and the inner annular pipe.

[0019] Preferably, the outer annular tube, the inner annular tube and the stirring shaft are connected via a plurality of connecting threaded tubes;

[0020] The connecting threaded pipe is provided with two second air holes which are respectively connected to the outer annular pipe and the inner annular pipe;

[0021] When the connecting threaded pipe is threadedly connected to the outer annular pipe and the inner annular pipe, the second air hole is located in the tube cavity of the outer annular pipe and the inner annular pipe respectively.

[0022] Preferably, an air flow channel is provided in the stirring shaft, a threaded air outlet hole communicating with the air flow channel is provided on the outer side wall of the stirring shaft, and the air inlet end of the communicating threaded pipe is threadedly connected to the threaded air outlet hole.

[0023] Preferably, the top of the fermentation and expansion tank body is connected to a feed pipe, and the top of the fermentation and expansion tank body is connected to an exhaust air pipe;

[0024] The bottom of the fermentation expansion tank is communicated with a discharge pipe.

[0025] Preferably, the top of the stirring motor is fixedly connected with a motor mounting seat.

[0026] The utility model has the following beneficial effects:

[0027] 1. When the motor drives rotation, the inner and outer layer distributed cylindrical stirring pipe is used to stir the nutrient solution in the tank.

[0028] 2. During the rotation stirring process, air flow stirring is accompanied, so that the dispersion degree of the nutrient substance and the expanded microorganism is greatly improved through the mode, so as to increase the expansion yield of the microorganism. BRIEF DESCRIPTION OF DRAWINGS

[0029] In order to more clearly illustrate the technical scheme in the embodiments of the utility model or the prior art, the drawings needed to be used in the embodiment or the prior art description will be briefly introduced below, and obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can be obtained according to the structure shown in these drawings without creative labor.

[0030] Figure 1 It is the overall structure schematic view in the embodiment of the utility model;

[0031] Figure 2 It is the culture solution stirring mechanism structure schematic view in the embodiment of the utility model;

[0032] Figure 3 It is the structure schematic view of the cylindrical stirring pipe communicating with the outer annular pipe in the embodiment of the utility model;

[0033] Figure 4 It is the structure schematic view of the cylindrical stirring pipe in the embodiment of the utility model;

[0034] Figure 5 It is the structure schematic view of the pipe cavity of the outer annular pipe communicating with the cylindrical stirring pipe in the embodiment of the utility model;

[0035] Figure 6 It is the structure schematic view of the communicating screw pipe in the embodiment of the utility model;

[0036] Figure 7 This is a schematic diagram of the dispersed structure of the outer annular tube in an embodiment of the present utility model.

[0037] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0038] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0039] It should be noted that all directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present invention are only used to explain the relative position relationship, movement status, etc. between the various components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0040] In addition, in this utility model, the descriptions of "first" and "second" are for descriptive purposes only and should not be understood as indicating or implying their relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features specified as "first" and "second" may explicitly or implicitly include at least one of such features. In addition, the technical solutions between the various embodiments can be combined with each other, but this must be based on the fact that they can be implemented by ordinary technicians in this field. When the combination of technical solutions is mutually contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by this utility model.

[0041] Example 1

[0042] like Figures 1-7 As shown, a butyric acid bacteria culture tank device includes a fermentation and expansion tank body 1. Similar to the existing method, the top of the fermentation and expansion tank body 1 is connected to a feed pipe 11, and the top of the fermentation and expansion tank body 1 is connected to an exhaust air pipe 12 (during the working process, the oxygen in the tank body is discharged in advance through the exhaust air pipe 12, specifically, by nitrogen filling and discharge); the bottom of the fermentation and expansion tank body 1 is connected to a discharge pipe (with a valve installed).

[0043] In order to realize the stirring of the nutrient solution in the fermentation expansion process, the expanded microorganisms are dispersed to increase the space for the reproduction of microorganisms and avoid the defect that the reproduction space is too small due to the concentrated distribution of microorganisms, the culture solution stirring mechanism is assembled on the fermentation expansion tank body 1. Through the improvement of the culture solution stirring mechanism, the microorganisms are protected during the stirring process.

[0044] Specifically, during the stirring process of the culture solution stirring mechanism, the breaking effect of the stirring structure on the bacterial cell wall is reduced.

[0045] The specific structure of the culture solution stirring mechanism is as follows:

[0046] The culture solution stirring mechanism includes a forward and reverse stirring motor 21 installed at the upper position of the fermentation expansion tank body 1 (according to the existing mode, the top of the stirring motor 21 is fixedly connected with a motor mounting seat, and the motor is suspendedly fixed and installed through the motor mounting seat). According to the existing mode, the stirring motor 21 is installed with a stirring material shaft 22 (the same as the existing mode, the stirring material shaft 22 is rotatably connected to the tank body), and a cylindrical stirring pipe structure is installed on the stirring material shaft 22.

[0047] Specifically, the cylindrical stirring pipe structure includes a plurality of cylindrical stirring pipes 26 with cylindrical structures, and an annular pipe is installed at the top and bottom of each cylindrical stirring pipe 26, specifically, an outer annular pipe 23 and an inner annular pipe 24 concentrically arranged with the outer annular pipe 23 are fixedly assembled and connected at the top and bottom of each cylindrical stirring pipe 26.

[0048] The outer annular pipe 23 and the inner annular pipe 23 are fixedly installed on the stirring material shaft 22 through a connecting pipe. At the same time, a plurality of cylindrical stirring pipes 26 distributed in a ring are fixedly installed between the outer annular pipes 23 and the inner annular pipes.

[0049] During the working process, when the motor is driven to rotate, the inner and outer layer distributed cylindrical stirring pipes 26 are used to stir the nutrient solution in the tank body. Since the cylindrical stirring pipes 26 with cylindrical structures do not form a large shear force during the stirring process (the traditional impeller and stirring paddle structure forms a large shear force, which leads to the breaking of the cell wall and the death of the microorganisms), the nutrient solution is fully dispersed while being stirred. During the dispersion process, the expanded and reproduced microorganisms are fully dispersed in the nutrient solution.

[0050] Embodiment 2

[0051] As shown in Figures 1-7 Based on the structure of embodiment 1, the butyric acid bacteria culture tank device further includes an air flow stirring structure opened on the culture solution stirring mechanism. During the rotation and stirring of the cylindrical stirring pipes 26, the air flow stirring structure pumps nitrogen into the fermentation expansion tank body 1 for air flow stirring.

[0052] The air flow stirring is used to further increase the dispersion uniformity of the nutrient solution during the working process, and at the same time, the oxygen dissolved in the nutrient solution is continuously removed during the air flow stirring process.

[0053] Specifically, the airflow stirring structure includes a plurality of nitrogen exhaust holes 261 opened on the cylindrical stirring tube 26 .

[0054] At the same time, the cylindrical stirring tube 26 is connected to the corresponding outer annular tube 23 and the inner annular tube 24; the specific connection method is: the top and bottom of the cylindrical stirring tube 26 are respectively integrally formed with a threaded connecting tube 262, and the threaded connecting tube 262 is threadedly connected to the corresponding outer annular tube 23 and the inner annular tube 24.

[0055] Furthermore, the threaded connecting tube 262 is provided with a first air hole 2621, which communicates with the lumens of the outer annular tube 23 and the inner annular tube 24. When the threaded connecting tube 262 is threadedly fastened to the outer annular tube 23 and the inner annular tube 24, the first air hole 2621 communicates with the lumens of the outer annular tube 23 and the inner annular tube 24. Furthermore, the threaded connecting tube 262 is threadedly connected to a nut that is locked onto the outer annular tube 23 and the inner annular tube 24.

[0056] The outer annular tube 23 and the inner annular tube 24 are in communication with each other, and the outer annular tube 23 and the inner annular tube 24 are in communication with the stirring shaft 22 via two communicating threaded tubes 25 .

[0057] Specifically, the connecting threaded tube 25 has two second air holes 251 that connect to the outer annular tube 23 and the inner annular tube 24, respectively. (When the connecting threaded tube 25 is threadedly connected to the outer annular tube 23 and the inner annular tube 24, the second air holes 251 are located in the tube lumens A of the outer annular tube 23 and the inner annular tube 24, respectively.) After the connecting threaded tube 25 is threadedly connected from the outer annular tube 23 to the inner annular tube 24, it is threaded through the inner annular tube 24 and then threaded onto the stirring shaft 22. This ensures that the outer annular tube 23 and the inner annular tube 24 are connected and fixed to the stirring shaft 22.

[0058] Specifically, according to the existing stirring shaft ventilation method, an air flow channel (not shown in the figure) is opened in the above-mentioned stirring shaft 22, and a threaded air outlet hole connected to the air flow channel is opened on the outer wall of the stirring shaft 22, and the air inlet end of the connecting threaded pipe 25 is threadedly connected to the threaded air outlet hole.

[0059] The outer annular tube 23 and the inner annular tube 24 are connected to the stirring shaft 22, and the upper end of the stirring shaft 22 is connected to a nitrogen inlet pipe 221 (the nitrogen inlet pipe is connected to an external nitrogen source, such as a nitrogen bottle, through a hose. The hose connection facilitates the motor to drive forward and reverse rotation stirring).

[0060] During operation, nitrogen first enters the stirring shaft 22, is then diverted through the connecting threaded tube 25, and enters the outer annular tube 23 and the inner annular tube 24. Finally, it is diverted into each cylindrical stirring tube 26, and then diverted from the cylindrical stirring tube 26 and pumped into the nutrient solution in the tank. In this way, the stirred nitrogen is fully dispersed into the nutrient solution for stirring.

[0061] The rotary stirring process is accompanied by air flow stirring, so this method greatly improves the dispersion of nutrients and cultured microorganisms, thereby increasing the culture yield of microorganisms.

[0062] Of course, the above description is not a limitation of the present invention, and the present invention is not limited to the above examples. Changes, modifications, additions or substitutions made by technicians in this technical field within the essential scope of the present invention should also fall within the scope of protection of the present invention.

Claims

1. A butyric acid bacteria culture tank device, characterized in that: The fermentation and expansion tank body is equipped with a culture liquid stirring mechanism, which reduces the breaking effect of the stirring structure on the bacterial cell wall during the stirring process of the culture liquid stirring mechanism; The culture liquid stirring mechanism includes a stirring motor installed above the fermentation and expansion tank body, the stirring motor is equipped with a stirring shaft, and the stirring shaft is equipped with a cylindrical stirring tube structure; The cylindrical stirring tube structure includes a plurality of cylindrical stirring tubes, the top and bottom of each cylindrical stirring tube are respectively provided with an annular tube, and the inner side wall of each annular tube is fixedly mounted on the stirring shaft via a connecting tube; The butyric acid bacteria culture tank device also includes an airflow stirring structure provided on the culture liquid stirring mechanism. When the cylindrical stirring tube rotates to stir the culture liquid, the airflow stirring structure pumps nitrogen into the fermentation and expansion tank body to perform airflow stirring.

2. The butyric acid bacteria culture tank device according to claim 1, characterized in that The top and bottom of the cylindrical stirring tube are respectively fixedly assembled with an outer annular tube and an inner annular tube arranged concentrically with the outer annular tube; A plurality of cylindrical stirring tubes distributed around the circumference are fixedly installed between the outer annular tubes and the inner annular tubes.

3. The butyric acid bacteria culture tank device according to claim 1, characterized in that The air flow stirring structure includes a plurality of nitrogen exhaust holes opened on the cylindrical stirring tube; The cylindrical stirring tube is connected to the corresponding outer annular tube and the inner annular tube; The outer annular tube and the inner annular tube are connected to the stirring shaft. The upper end of the stirring shaft is connected to a nitrogen inlet pipe, and the nitrogen inlet pipe is connected to an external nitrogen source.

4. The butyric acid bacteria culture tank device according to claim 3, characterized in that: The top and bottom of the cylindrical stirring tube are respectively integrally formed with threaded connecting pipes, and the threaded connecting pipes are threadedly connected to the corresponding outer annular tube and inner annular tube; The threaded connecting pipe is provided with a first air hole, which is connected to the lumens of the outer annular pipe and the inner annular pipe.

5. The butyric acid bacteria culture tank device according to claim 4, characterized in that: The threaded connection pipe is threadedly connected with a nut which is locked on the corresponding outer annular pipe and the inner annular pipe.

6. The butyric acid bacteria culture tank device according to claim 5, characterized in that: The outer annular tube, the inner annular tube and the stirring shaft are connected through a plurality of connecting threaded tubes; The connecting threaded pipe is provided with two second air holes which are respectively connected to the outer annular pipe and the inner annular pipe; When the connecting threaded pipe is threadedly connected to the outer annular pipe and the inner annular pipe, the second air hole is located in the tube cavity of the outer annular pipe and the inner annular pipe respectively.

7. The butyric acid bacteria culture tank device according to claim 6, characterized in that: An air flow channel is provided in the stirring shaft, a threaded air outlet hole communicating with the air flow channel is provided on the outer wall of the stirring shaft, and an air inlet end of the communicating threaded pipe is threadedly connected to the threaded air outlet hole.

8. The butyric acid bacteria culture tank device according to claim 1, characterized in that: The top of the fermentation and expansion tank body is connected to a feed pipe, and the top of the fermentation and expansion tank body is connected to an exhaust air pipe; The bottom of the fermentation and expansion tank body is connected with a discharge pipe.

9. The butyric acid bacteria culture tank device according to claim 1, characterized in that: The top of the stirring motor is fixedly connected with a motor mounting seat.

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