Culture dish for co-culture of stem cells

Abstract

The utility model discloses a culture dish for co-culture of stem cells. The culture dish comprises a base, the top end of the base is in threaded connection with a cover body, a placement structure is arranged on the outer side of the cover body, a ventilation structure is installed at the top end of the cover body, and a culture structure is installed in the base. According to the utility model, a plurality of stem cells needing to be co-cultured are respectively injected into the base and the small culture chamber according to respective characteristics, so that the plurality of cells can be cultured and incubated in the same culture system environment, and when substance exchange is needed among the cells, only a culture solution in the base needs to be added; the liquid level is higher than the polycarbonate film at the bottom of the small culture chamber, the polycarbonate film is high in hole density, more exchange can be completed through the film, the polycarbonate film has the excellent cell adhesion characteristic, various cells cannot be mixed together during sampling, separation and sampling are more convenient, and the sampling efficiency is improved. Therefore, the purpose that the culture dish for stem cell co-culture can conveniently separate various cells is achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a culture dish for co-culturing stem cells. Background Art

[0002] Stem cells are a type of cell with the potential for self-renewal and multidirectional differentiation. The self-renewal ability means that they can produce identical daughter cells through cell division while maintaining their own stable number, while the multidirectional differentiation potential enables them to differentiate into different types of cells under specific conditions, thus forming various tissues and organs. Depending on their differentiation ability, stem cells can be divided into totipotent stem cells, pluripotent stem cells and unipotent stem cells. During embryonic development, stem cells continue to differentiate, gradually forming various tissues and organs, and building complex biological structures. In adults, stem cells are also present in many tissues and organs, such as bone marrow, fat, liver, etc., playing a role in replenishing and repairing damaged or aging cells, and maintaining the normal function and structural integrity of tissues and organs. Stem cell co-culture refers to the incubation of two or more stem cells or different types of cells in the same culture system environment in vitro to approximately simulate the microenvironment in which cells are generated in the body.

[0003] To this end, China has filed patent application number CN206089675U, disclosing a culture dish comprising a base and a lid for use with the base. At least two retaining bars are disposed on the bottom plane of the base's inner cavity. One end of each retaining bar is located at the center of the bottom plane, and the other end is connected to the inner surface of the base's sidewall. A barrier that cooperates with the retaining bar is disposed within the inner cavity of the lid. This culture dish can simultaneously culture multiple bacteria, ensuring that no contamination occurs, reducing the cost of the experiment. Furthermore, inoculating bacteria on only one dish significantly speeds up the inoculation process and reduces time costs.

[0004] Today's stem cell co-culture dishes can basically meet people's needs, but there are still some problems. The specific problems are as follows:

[0005] 1. It is difficult to conveniently separate multiple cells using culture dishes for stem cell co-culture. For stem cell co-culture, two or more stem cells need to be placed in the same culture system environment for culture and incubation. During the culture process, different cells may mix with each other, thus affecting subsequent collection and analysis.

[0006] 2. It is difficult to adjust the permeability of culture dishes used for stem cell co-culture. During the co-culture process, stem cells require sufficient oxygen and nutrients to maintain their growth and differentiation. In stem cell co-culture, different types of cells may have different requirements for oxygen, and the permeability of the culture dish needs to be adjusted according to the needs. Utility Model Content

[0007] The utility model aims to provide a culture dish for stem cell co-culture, so as to solve the defects of existing culture dishes for stem cell co-culture, namely, difficulty in conveniently separating multiple cells and difficulty in adjusting the air permeability.

[0008] In order to solve the above technical problems, the present utility model provides the following technical solutions: a culture dish for stem cell co-culture, comprising a base;

[0009] The top end of the base is threadedly connected to a cover body, and a placement structure is provided on the outer side of the cover body;

[0010] The top of the cover is provided with a breathable structure, and the interior of the base is provided with a culture structure;

[0011] The culture structure includes a culture chamber, a third air hole and a polycarbonate film. The culture chamber is clamped inside the base. The third air hole is evenly opened inside the top of the culture chamber. The polycarbonate film is bonded to the bottom of the culture chamber.

[0012] When in use, first rotate the cover body in a sterile environment to separate the cover body from the base, take the culture chamber out of the base, and use a rubber-tipped dropper or other instrument to inject the culture fluid and the cells to be cultured into the base and the culture chamber respectively. When the two need to exchange substances, the culture fluid level in the base can be allowed to be higher than the bottom of the culture chamber. Then, the culture chamber is snapped back onto the base and the cover body is screwed on. The adjustment cap on the top of the cover body is rotated according to the growth requirements of the cultured cells to change the overlapping area of ​​the first air hole and the second air hole, thereby changing the air permeability of the culture dish. Finally, the culture dish is placed in the incubator. Multiple culture dishes can be stacked on each other by using the engagement of the convex ring and the concave ring.

[0013] Furthermore, external threads are evenly provided on the outer side wall of the top of the base, and internal threads that cooperate with the external threads are evenly provided on the inner side wall of the cover body. The base and the cover body are threadedly connected, making the connection between the cover body and the base more stable.

[0014] Furthermore, the placement structure includes anti-slip grooves, placement convex rings and placement concave rings. The anti-slip grooves are arranged on the outer side wall of the cover body, the placement convex rings are integrally formed at the top end of the cover body, and the placement concave rings are integrally formed at the bottom end of the base. The placement concave rings and the placement convex rings are engaged with each other, which can increase stability during movement and save storage space.

[0015] Furthermore, the ventilation structure includes an adjustment cover, a first ventilation hole, a second ventilation hole and a ventilation membrane. The adjustment cover is movably installed on the top of the cover body. The first ventilation holes are evenly opened inside the adjustment cover. The ventilation membrane is installed inside the cover body, and the ventilation permeability of the culture dish can be changed according to the culture requirements.

[0016] Furthermore, a slider is provided at the bottom end of the adjustment cover, and a slide groove is provided on the outer side of the top end of the cover body. The adjustment cover and the cover body are slidably connected, and the overlapping area of ​​the first air hole and the second air hole can be changed by rotating the adjustment cover.

[0017] Furthermore, second air holes are evenly opened inside the top of the cover body, and the second air holes are distributed in a ring shape, so that the gas exchange is more uniform.

[0018] Furthermore, a card block is provided on the outside of the culture chamber, and a card slot is provided on the top of the base. The culture chamber and the base are connected by a card engagement, so that the culture chamber can be stably placed in the base.

[0019] The utility model provides a culture dish for stem cell co-culture, which has the advantages that: by injecting multiple stem cells that need to be co-cultured into the base and the culture chamber according to their respective characteristics, multiple cells can be cultured and incubated in the same culture system environment. When substances need to be exchanged between cells, only the culture fluid in the base needs to be added, and the liquid level needs to be higher than the polycarbonate film at the bottom of the culture chamber. The polycarbonate film has a high pore density, which can complete more exchanges through the membrane, and has excellent cell adhesion properties. During sampling, multiple cells will not be mixed together, which is more convenient for separation and sampling. The third air vent can ensure the air permeability in the base, which is convenient for the growth of stem cells, thereby achieving the purpose of convenient separation of multiple cells in the stem cell co-culture culture dish.

[0020] A second air hole is opened on the cover body, and a rotatable adjustment cover is provided on the cover body. The first air hole is also opened on the adjustment cover. A breathable membrane is installed inside the cover body. The breathable membrane is a polytetrafluoroethylene filter membrane, which can ensure the entry of carbon dioxide and the discharge of oxygen, while preventing the entry of microorganisms and pollutants. The first air hole and the second air hole have the same shape. The air permeability inside the culture dish can be controlled by rotating the adjustment cover to change the overlapping area of ​​the first air hole and the second air hole, which is more convenient for cell culture, thereby achieving the purpose of being able to adjust the air permeability of the culture dish for stem cell co-culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic diagram of the three-dimensional explosion structure of the utility model;

[0022] Figure 2 This is a schematic diagram of the three-dimensional explosion structure of the utility model;

[0023] Figure 3 It is a top view structural schematic diagram of the utility model;

[0024] Figure 4 It is a front view sectional structure schematic diagram of the utility model;

[0025] Figure 5 It is a Figure 4 It is a local sectional enlarged structure schematic diagram of A place in the middle.

[0026] The reference signs in the drawing are explained as follows: 1, base; 2, cover; 3, placing structure; 301, anti-skid line; 302, placing convex ring; 303, placing concave ring; 4, ventilation structure; 401, adjusting cover; 402, first ventilation hole; 403, second ventilation hole; 404, ventilation film; 5, culture structure; 501, culture chamber; 502, third ventilation hole; 503, polycarbonate film. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0028] Please refer to Figures 1-5 The utility model provides an embodiment: a culture dish for stem cell co-culture, including base 1.

[0029] The top end of base 1 is screw connected with cover 2, and the outer side wall of the top end of base 1 is uniformly provided with external threads, and the inner side wall of cover 2 is uniformly provided with internal threads matched with the external threads, and base 1 and cover 2 are screw connected.

[0030] The outer side of cover 2 is provided with placing structure 3, and placing structure 3 includes anti-skid line 301, placing convex ring 302 and placing concave ring 303, anti-skid line 301 is arranged on the outer side wall of cover 2, placing convex ring 302 is integrally formed at the top end of cover 2, and placing concave ring 303 is integrally formed at the bottom end of base 1.

[0031] Referring to the drawings Figures 1-5As shown, the outer diameter of the placement concave ring 303 is smaller than the inner diameter of the placement convex ring 302. When multiple culture dishes are used for culture at the same time and the culture dishes are transferred and taken, the multiple culture dishes can be stacked and placed. The placement concave ring 303 and the placement convex ring 302 are engaged with each other, which can increase stability during movement and save storage space. Anti-slip grooves 301 are provided on the outer side of the cover body 2. When taking the culture dish and opening and closing the culture dish, the friction between the culture dish and the hand is greatly enhanced, which is more convenient to operate and reduces the possibility of the culture dish slipping out of the hand and spilling.

[0032] A breathable structure 4 is installed at the top of the cover body 2, and the breathable structure 4 includes an adjusting cover 401, a first breathable hole 402, a second breathable hole 403 and a breathable membrane 404. The adjusting cover 401 is movably installed at the top of the cover body 2, a slider is provided at the bottom end of the adjusting cover 401, and a slide groove is provided on the outer side of the top of the cover body 2. The adjusting cover 401 is slidably connected to the cover body 2, and the first breathable holes 402 are evenly opened inside the adjusting cover 401, and the second breathable holes 403 are evenly opened inside the top of the cover body 2. The second breathable holes 403 are distributed in a ring shape, and the breathable membrane 404 is installed inside the cover body 2.

[0033] Refer to the attached Figures 1-5 As shown, a second air hole 403 is provided on the cover body 2, a rotatable adjustment cover 401 is provided on the cover body 2, a first air hole 402 is also provided on the adjustment cover 401, and an air permeable membrane 404 is installed inside the cover body 2. The air permeable membrane 404 is a polytetrafluoroethylene filter membrane, which can ensure the entry of carbon dioxide and the discharge of oxygen while preventing the entry of microorganisms and pollutants. The first air hole 402 and the second air hole 403 have the same shape. The air permeability inside the culture dish can be controlled by rotating the adjustment cover 401 to change the overlapping area of ​​the first air hole 402 and the second air hole 403, which is more convenient for cell culture.

[0034] A culture structure 5 is installed inside the base 1, and the culture structure 5 includes a culture chamber 501, a third air hole 502 and a polycarbonate film 503. The culture chamber 501 is snapped into the inside of the base 1, a card block is provided on the outside of the culture chamber 501, and a card slot is provided at the top of the base 1. The culture chamber 501 and the base 1 are snap-connected, and the third air holes 502 are evenly opened inside the top of the culture chamber 501. The polycarbonate film 503 is bonded to the bottom of the culture chamber 501.

[0035] Refer to the attached Figure 2 and attached Figures 4-5The various stem cells which need to be co-cultured are injected into the base 1 and the culture chamber 501 according to their respective characteristics, so that the various cells can be cultured in the same culture system environment, and when material exchange between the cells is needed, only the culture solution in the base 1 needs to be added to make the liquid level higher than the polycarbonate film 503 at the bottom of the culture chamber 501, the polycarbonate film 503 has a higher hole density, more exchange can be completed through the film, and the polycarbonate film 503 has excellent cell adhesion characteristics, and when sampling, the various cells will not be mixed together, which is more convenient for separation and sampling, and the third gas permeable hole 502 can ensure the gas permeability of the base 1, which is convenient for the growth of stem cells.

[0036] Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments or make equivalent replacement to part of the technical features, and any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model should be included in the protection scope of the utility model.

Claims

1. A culture dish for stem cell co-culture, comprising a base (1); Its characteristics are: The top end of the base (1) is threadedly connected to a cover body (2), and a placement structure (3) is provided on the outer side of the cover body (2); A ventilation structure (4) is installed on the top of the cover (2), and a culture structure (5) is installed inside the base (1); The culture structure (5) comprises a culture chamber (501), a third air vent (502) and a polycarbonate film (503); the culture chamber (501) is snap-fitted to the interior of the base (1); the third air vent (502) is evenly arranged on the interior of the top of the culture chamber (501); and the polycarbonate film (503) is bonded to the bottom of the culture chamber (501).

2. The stem cell co-culture culture dish according to claim 1, characterized in that: External threads are evenly arranged on the outer side wall of the top of the base (1), and internal threads that cooperate with the external threads are evenly arranged on the inner side wall of the cover (2). The base (1) and the cover (2) are connected by threads.

3. The stem cell co-culture culture dish according to claim 1, characterized in that: The placement structure (3) comprises an anti-slip pattern (301), a placement convex ring (302) and a placement concave ring (303); the anti-slip pattern (301) is arranged on the outer side wall of the cover body (2); the placement convex ring (302) is integrally formed at the top end of the cover body (2); and the placement concave ring (303) is integrally formed at the bottom end of the base (1).

4. The stem cell co-culture culture dish according to claim 1, characterized in that: The ventilation structure (4) comprises an adjustment cover (401), a first ventilation hole (402), a second ventilation hole (403) and a ventilation membrane (404); the adjustment cover (401) is movably mounted on the top of the cover body (2); the first ventilation holes (402) are evenly arranged inside the adjustment cover (401); and the ventilation membrane (404) is mounted inside the cover body (2).

5. The stem cell co-culture culture dish according to claim 4, characterized in that: The bottom end of the regulating cover (401) is provided with a slider, the outer side of the top end of the cover body (2) is provided with a slide groove, and the regulating cover (401) and the cover body (2) are slidably connected.

6. The stem cell co-culture culture dish according to claim 4, characterized in that: Second air holes (403) are evenly arranged inside the top end of the cover body (2), and the second air holes (403) are distributed in a ring shape.

7. The culture dish for stem cell co-culture according to claim 1, characterized in that: A card block is provided on the outside of the culture chamber (501), and a card slot is provided on the top of the base (1). The culture chamber (501) and the base (1) are connected in a card-engaging manner.

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