Disposable high-density cell culture plate
By designing a disposable high-density cell culture plate and adopting a double breathable membrane structure, the problem of insufficient oxygen supply is solved, and rapid cell expansion and efficient culture are achieved.
Patent Information
- Application Number
- CN202422748379.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-12
- Publication Date
- 2025-10-10
- Estimated Expiration
- 2034-11-12
AI Technical Summary
Existing cell culture dishes cannot ensure the entry of oxygen, resulting in poor cell culture results.
A disposable high-density cell culture plate was designed, which includes a culture plate placement box and a culture plate cover. Multiple culture tubes and a waterproof breathable membrane are set up to achieve double-permeable culture from top to bottom, ensuring that cells can fully absorb oxygen.
High-density cell culture and rapid cell expansion are achieved, which improves the culture effect.
Smart Images
Figure CN223422694U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture, in particular to a disposable high-density cell culture plate. Background Art
[0002] Cell culture is a laboratory technique used to artificially grow cells outside the body. It's an important tool for research in fields such as biology, medicine, and bioengineering. Isolated cells are placed in a culture dish or flask and placed in a CO2 incubator. The incubator's conditions, such as temperature, humidity, and CO2 concentration, are designed to mimic the in vivo environment.
[0003] Currently, most cell cultures directly place separated cells into culture dishes, install a cover on top of the dish, and then place it into an incubator. However, after some covers are sealed, oxygen cannot enter the dish to allow the cells to expand. This makes it impossible to culture the cells completely, resulting in poor cell culture results. Utility Model Content
[0004] The utility model aims to solve the shortcomings in the prior art and provides a disposable high-density cell culture plate.
[0005] In order to achieve the above purpose, the present invention adopts the following technical solutions:
[0006] A disposable high-density cell culture plate comprises a culture plate placement box, wherein a cavity is defined in the culture plate placement box, a cell culture plate mechanism is disposed in the cavity, and the cell culture plate mechanism comprises a plate body, which is horizontally fixed to the top of the cavity, and a plurality of culture tubes are evenly arranged on the plate body;
[0007] A culture cavity is provided in the culture tube, a second waterproof and breathable membrane is provided at the bottom of the culture tube located at the culture cavity, and a culture plate cover is horizontally placed above the cell culture plate mechanism in the cavity.
[0008] In addition, a preferred structure is that an upper cover is installed above the culture plate placement box.
[0009] In addition, the preferred structure is that slots are symmetrically opened on the side walls on both sides of the top of the culture plate placement box, a supporting bottom plate is provided on the side of the lower surface of the culture plate placement box, and multiple ventilation holes are evenly opened on the lower wall of the culture plate placement box located in the cavity.
[0010] In addition, a preferred structure is that an inserting plate is vertically fixedly connected to the middle of the two side edges of the upper cover plate, and the inserting plate is horizontally inserted into the slot.
[0011] In addition, the preferred structure is that a plurality of annular protrusions are evenly arranged on the culture plate cover, each annular protrusion is coaxial with the corresponding culture tube below, a first waterproof and breathable membrane is provided on the annular protrusion, and a plurality of protrusions are evenly arranged in the vertical direction on the inner walls on both sides of the top side plate of the culture plate cover.
[0012] In addition, a preferred structure is that a sealing gasket is attached to the surface of the annular protrusion facing the culture tube.
[0013] In addition, a preferred structure is that a circle of rubber pad is attached to the outer side wall of the culture plate cover, and the rubber pad is in contact with the inner wall of the cavity.
[0014] The beneficial effects of the utility model are as follows: the utility model provides a plurality of culture tubes on the plate body, so that the cell culture plate can perform high-density culture and culture multiple cells at one time; and the first waterproof breathable membrane and the second waterproof breathable membrane are provided to realize upper and lower double-ventilation culture; during the culture process, the cells settle on the bottom breathable membrane, and can fully absorb the oxygen from the first waterproof breathable membrane and can also fully breathe the oxygen entering from the second waterproof breathable membrane at the bottom, so that the cells can be rapidly expanded and the effect of cell culture is improved. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 This is a structural diagram of the upper cover plate being installed on the culture plate placement box;
[0016] Figure 2 This is a schematic diagram of the structure after the upper cover, culture plate cover and culture plate placement box are disassembled;
[0017] Figure 3 Schematic diagram of the structure of the upper cover;
[0018] Figure 4 Schematic diagram of the structure of the culture plate cover Figure 1 ;
[0019] Figure 5 for Figure 4 A magnified view of middle A;
[0020] Figure 6 Schematic diagram of the structure of the culture plate cover Figure 2 ;
[0021] Figure 7 for Figure 6 Enlarged view of middle B;
[0022] Figure 8 This is a schematic diagram of the structure after the cell culture plate mechanism and the culture plate placement box are separated;
[0023] Figure 9 Schematic diagram of the structure of the culture plate placement box;
[0024] Figure 10 This is a schematic diagram of the structure of the local culture plate placement box;
[0025] Figure 11 Schematic diagram of the structure of the culture tube.
[0026] In the figure: 1 culture plate placement box, 11 cavity, 12 slot, 13 support base, 14 vent, 2 upper cover, 21 insert plate, 3 culture plate cover, 31 first waterproof breathable membrane, 32 circular protrusion, 33 protrusion, 4 rubber pad, 5 sealing gasket, 6 cell culture plate mechanism, 61 culture tube, 611 culture cavity, 612 second waterproof breathable membrane, 62 plate body. DETAILED DESCRIPTION
[0027] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.
[0028] Reference Figures 1-11 A disposable high-density cell culture plate includes a culture plate placement box 1, a cavity 11 is opened in the culture plate placement box 1, a cell culture plate mechanism 6 is arranged in the cavity 11, the cell culture plate mechanism 6 includes a plate body 62, the plate body 62 is horizontally fixed on the top of the cavity 11, a plurality of culture tubes 61 are evenly arranged on the plate body 62, a culture cavity 611 is opened in the culture tube 61, the culture tube 61 is located at the bottom of the culture cavity 611 and is provided with a second waterproof breathable membrane 612, a culture plate cover 3 is horizontally placed above the cell culture plate mechanism 6 in the cavity 11, the second waterproof breathable membrane 612 facilitates the cells to fully breathe the oxygen entering from the second waterproof breathable membrane 612, so that the cells can proliferate rapidly.
[0029] Moreover, an upper cover plate 2 is installed above the culture plate placement box 1, and dust can be placed on the upper cover plate 2 so as to fall vertically onto the culture plate cover plate 3 below.
[0030] In addition, slots 12 are symmetrically provided on the side walls on both sides of the top of the culture plate placement box 1, a supporting bottom plate 13 is provided on the side of the lower end surface of the culture plate placement box 1, and a plurality of ventilation holes 14 are evenly provided on the lower wall of the culture plate placement box 1 located in the cavity 11, which facilitate the entry of oxygen.
[0031] At the same time, an inserting plate 21 is vertically fixedly connected to the middle of the two side edges of the upper cover plate 2 , and the inserting plate 21 is horizontally inserted into the slot 12 , which facilitates the installation of the upper cover plate 2 .
[0032] Moreover, a plurality of annular protrusions 32 are arranged uniformly on the culture plate cover plate 3, each annular protrusion 32 is coaxial with the corresponding culture tube 61 below, a first waterproof and breathable film 31 is arranged on the annular protrusion 32, a plurality of protrusions 33 are arranged uniformly on the inner walls of the two sides of the top side plate body of the culture plate cover plate 3 in the vertical direction, a sealing gasket 5 is attached to the side surface of the annular protrusion 32 facing the culture tube 61, and the sealing gasket 5 facilitates improving the sealing between the annular protrusion 32 and the culture tube 61.
[0033] In addition, a rubber pad 4 is attached to the outer side wall of the culture plate cover plate 3, the rubber pad 4 is in contact with the inner wall of the cavity 11, and the rubber pad 4 facilitates increasing the damping between the culture plate cover plate 3 and the inner wall of the cavity 11, so that the culture plate cover plate 3 is installed more firmly.
[0034] In the embodiment, when batch cultivation of cells is required, the device can be used, the plug-in plate 21 of the upper cover plate 2 is pulled out from the plug-in slot 12 in a sterile environment, so that the upper cover plate 2 is quickly disassembled, then the culture plate cover plate 3 is pulled out through the protrusions 33, the culture plate cover plate 3 is conveniently pried out from the inner cavity by the operator, then the operator uses a pipette to inject the cell solution to be cultivated into each culture tube 61, after each culture tube 61 is injected with the cell solution to be cultivated, the culture plate cover plate 3 can be re-docked in the cavity 11, and at this time, it can be placed in a sterile environment for cultivation.
[0035] In this way, under the cooperation of the first waterproof and breathable film 31 and the second waterproof and breathable film 612, double-breathable culture is realized, the cells settle on the bottom breathable film during the cultivation process, can fully absorb oxygen from the first waterproof and breathable film 31, and can fully breathe oxygen entering from the bottom second waterproof and breathable film 32, so that the cells can be rapidly expanded.
[0036] The upper cover plate 2 is detachable, which prevents dust from falling on the culture plate cover plate 2 during transportation, and the cell culture plate as a whole is disposable, and after the cells are cultivated, it needs to be discarded.
[0037] In the utility model, a plurality of culture tubes 61 are arranged on the plate body 62, so that the cell culture plate can be cultured at high density, a plurality of cells can be cultivated at one time, the first waterproof and breathable film 31 and the second waterproof and breathable film 612 are arranged, double-breathable culture is realized, the cells settle on the bottom breathable film during the cultivation process, can fully absorb oxygen from the first waterproof and breathable film 31, and can fully breathe oxygen entering from the bottom second waterproof and breathable film 32, so that the cells can be rapidly expanded, and the effect of cell cultivation is improved.
[0038] The above is only a preferred specific implementation method of the present invention, but the protection scope of the present invention is not limited to this. Any technician familiar with the technical field within the technical scope disclosed by the present invention can make equivalent replacements or changes based on the technical solution and utility model concept of the present invention, which should be covered by the protection scope of the present invention.
Claims
1. A disposable high-density cell culture plate, comprising a culture plate placement box (1), characterized in that: The culture plate placement box (1) is provided with a cavity (11), a cell culture plate mechanism (6) is provided in the cavity (11), and the cell culture plate mechanism (6) includes a plate body (62), the plate body (62) is horizontally fixed on the top of the cavity (11), and a plurality of culture tubes (61) are evenly arranged on the plate body (62); A culture cavity (611) is provided in the culture tube (61), a second waterproof and breathable membrane (612) is provided at the bottom of the culture cavity (611) of the culture tube (61), and a culture plate cover (3) is horizontally placed above the cell culture plate mechanism (6) in the cavity (11).
2. A disposable high-density cell culture plate according to claim 1, characterized in that: An upper cover plate (2) is installed above the culture plate placement box (1).
3. A disposable high-density cell culture plate according to claim 2, characterized in that: Slots (12) are symmetrically provided on the side walls on both sides of the top of the culture plate placement box (1), a supporting bottom plate (13) is provided on the side edge of the lower surface of the culture plate placement box (1), and a plurality of vents (14) are evenly provided on the lower wall of the culture plate placement box (1) located in the cavity (11).
4. A disposable high-density cell culture plate according to claim 2, characterized in that: Insertion plates (21) are vertically fixedly connected to the middle of the two side edges of the upper cover plate (2), and the insertion plates (21) are horizontally inserted into the slots (12).
5. The disposable high-density cell culture plate according to claim 1, characterized in that: The culture plate cover (3) is provided with a plurality of circular protrusions (32) arranged evenly, each circular protrusion (32) being coaxial with the corresponding culture tube (61) below, and a first waterproof and breathable membrane (31) is provided on the circular protrusion (32). A plurality of protrusions (33) are evenly arranged in a vertical direction on both sides of the inner wall of the top side plate body of the culture plate cover (3).
6. The disposable high-density cell culture plate according to claim 5, characterized in that: A sealing gasket (5) is attached to the surface of the annular protrusion (32) on one side facing the culture tube (61).
7. The disposable high-density cell culture plate according to claim 5, characterized in that: A circle of rubber pad (4) is attached to the outer side wall of the culture plate cover (3), and the rubber pad (4) is in contact with the inner wall of the cavity (11).