Tube frame structure for fluorescent quantitative PCR (Polymerase Chain Reaction)

Abstract

The utility model discloses a fluorescent quantitative PCR tube frame structure, and relates to the field of fluorescent quantitative PCR equipment, the fluorescent quantitative PCR tube frame structure comprises a bottom frame, a movable cavity is formed in the bottom frame, a fixing block for fixing a test tube is movably arranged in the movable cavity, the top of the bottom frame is connected with a top frame, and the top frame is connected with a fluorescent quantitative PCR tube. The opposite sides of the bottom frame and the top frame form a hollow for storing test tubes; a threaded opening is formed in the center of the top of the top frame, an abutting plate used for sealing a test tube is arranged at the top of the top frame around the threaded opening, and a plurality of L-shaped blocks used for fixing the abutting plate are elastically connected into the threaded opening. The test tube fixing device can perform multiple fixation on the test tubes to prevent collision damage, and meanwhile, the test tubes are in a sealed state to effectively protect samples from scattering.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of fluorescent quantitative PCR equipment, and in particular to a tube rack structure for fluorescent quantitative PCR. BACKGROUND

[0002] The fluorescent quantitative PCR instrument is an analytical instrument used in the fields of chemistry, biology, agronomy and forestry. The fluorescent quantitative PCR instrument is composed of a fluorescent quantitative system and a computer. The fluorescent quantitative system is used to monitor the cycle process. The computer connected with the real-time device collects the fluorescence data. The tube rack structure is a tool used for storing samples in the fluorescent quantitative PCR instrument. The existing tube rack structure for fluorescent quantitative PCR is usually connected to one side of the instrument main body and has simple supporting and fixing effects.

[0003] For example, in the application No. CN202323522247.3, an amplification tube rack for fluorescent quantitative PCR is still insufficient in actual use.

[0004] The tube rack is connected to one side of the fluorescent quantitative PCR body, and the test tube is inserted into the fixed frame. The fixed frame is moved along the limiting groove by the limiting seat. However, the fluorescent quantitative PCR body is centrifuged or the fixed frame is moved, which is easy to shake, so as to cause the sample in the test tube to spill. Different types of samples need different sizes of test tubes for storage, so that the test tube cannot completely fit the inner wall of the fixed frame. When the fixed frame shakes, the test tube is broken by collision. Practical new type content

[0005] In order to improve the problem that the conventional tube rack structure cannot seal the test tube, the sample spills, the test tube has poor fixing, and the test tube is broken by collision, the present application provides a tube rack structure for fluorescent quantitative PCR.

[0006] The tube rack structure for fluorescent quantitative PCR provided by the present application adopts the following technical scheme:

[0007] A tube rack structure for fluorescent quantitative PCR, comprising a bottom frame arranged on one side of a detection instrument, an activity cavity is formed in the bottom frame, a fixing block for fixing a test tube is movably arranged in the activity cavity, a top frame is connected to the top of the bottom frame, and a hollow space is formed on the opposite side of the bottom frame and the top frame for storing the test tube.

[0008] A threaded hole is arranged at the center of the top of the top frame, a pressing plate for sealing the test tube is arranged around the threaded hole on the top of the top frame, and a plurality of L-shaped blocks for fixing the pressing plate are elastically connected in the threaded hole.

[0009] By adopting the above technical solution, the bottom frame and the top frame provide storage space for the test tubes, while the middle parts of the bottom frame and the top frame are hollow, so as to facilitate air flow to maintain a constant temperature. The L-shaped block fixes the pressing plate, so that the pressing plate applies downward pressure on the top of the test tube, ensuring the sealing state of the test tube. At the same time, it cooperates with the fixed block to move along the movable cavity and clamp the bottom of the test tube, effectively clamping and fixing the test tube between the bottom frame and the top frame to prevent the test tube from collision and damage.

[0010] Preferably, a plurality of shrinkage holes are provided on the upper end surface of the bottom frame, and the plurality of shrinkage holes all pass through the bottom frame and communicate with the active cavity.

[0011] By adopting the above technical solution, when the bottom of the test tube is inserted into the shrinkage hole, the inclined surface inside the shrinkage hole assists in fixing the test tube. At the same time, the shrinkage hole is connected to the movable cavity, which facilitates the subsequent fixing structure to squeeze and fix the test tube through the shrinkage hole.

[0012] Preferably, a threaded hole is provided at the center of the upper end surface of the bottom frame, and the threaded hole passes through the bottom frame and is connected with the active cavity.

[0013] By adopting the above technical solution, the threaded hole provides a fulcrum for the connection between the bottom frame and the top frame, and the threaded hole is connected to the movable cavity, thereby reserving movable space for the subsequent pushing of the extrusion block.

[0014] Preferably, the fixing block is provided with arc-shaped notches at the positions of the multiple shrinkage holes, and the fixing block is provided with an extrusion block at the position of the threaded hole, and the extrusion block is movably inserted in the threaded hole.

[0015] By adopting the above technical solution, the extrusion block is pushed by the thrust to push the fixed block, so that the fixed block moves in the movable cavity. As the fixed block moves, the arc-shaped notch is pressed into the shrinkage hole to extrude and fix the test tube.

[0016] Preferably, a plurality of springs are connected to a side of the outer surface of the fixing block close to the inner wall of the active cavity, and ends of the plurality of springs away from the fixing block are fixedly connected to the inner wall of the active cavity.

[0017] By adopting the above technical solution, the spring applies a rebound force to the fixed block that has lost the influence of the thrust, so that the fixed block moves in the opposite direction in the movable cavity, thereby returning to its original position.

[0018] Preferably, a plurality of L-shaped grooves are formed on the outer surface of the threaded opening, and the plurality of L-shaped grooves are movably connected to a plurality of L-shaped blocks, and a threaded rod is threadedly connected to the inside of the threaded opening.

[0019] By adopting the above technical solution, the threaded rod is rotated into the threaded mouth to apply thrust to the L-shaped block, causing the L-shaped block to translate outward along the L-shaped groove, thereby protruding from the threaded mouth. The protruding part of the L-shaped block limits the top of the clamping plate to form a fixation.

[0020] Preferably, the top frame bottom is provided with a plug hole at the position of each shrink hole.

[0021] By using the above technical scheme, the test tube is inserted into the shrink hole through the plug hole, thereby being stored, and the plug hole and the shrink hole respectively fix the two ends of the test tube transversely.

[0022] Preferably, the middle part of the abutting plate is provided with a plug slot, the inner wall of the plug slot is movably inserted into the threaded port, and the bottom of the abutting plate is fixedly provided with a plurality of sealing plugs at the position of each plug hole.

[0023] By using the above technical scheme, the plug slot and the threaded port are inserted to be limited, preventing the abutting plate from rotating and deviating, and meanwhile, the plurality of sealing plugs and the plug hole are inserted to form a seal, thereby sealing the tube opening of the test tube.

[0024] In summary, the present application has at least one of the following beneficial technical effects:

[0025] 1. The threaded rod is rotated into the threaded port, thereby pushing the L-shaped block to limit the abutting plate, fixing the abutting plate on the top of the top frame, thereby allowing the sealing plug to be inserted into the tube opening of the test tube to form a seal to prevent the sample from spilling, and meanwhile, the abutting plate is pressed on the top of the test tube to form a fixation, and the threaded rod pushes the extrusion block, allowing the arc-shaped notch to be pressed into the shrink hole to extrude the bottom of the test tube, and the multiple fixation cooperations fix the test tube in the tube rack to prevent damage caused by collision of the test tube. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 is a three-dimensional schematic view of the present application;

[0027] Figure 2 is a side cross-sectional exploded view of the present application;

[0028] Figure 3 is an internal structure view of the bottom frame of the present application;

[0029] Figure 4 is a side cross-sectional view of the top frame of the present application.

[0030] Reference signs: 1, bottom frame; 2, top frame; 3, threaded port; 4, abutting plate; 5, shrink hole; 6, movable cavity; 7, threaded hole; 8, spring; 9, fixed block; 10, arc-shaped notch; 11, extrusion block; 12, L-shaped slot; 13, L-shaped block; 14, plug hole; 15, sealing plug; 16, plug slot; 17, threaded rod.

[0031] 10, arc-shaped notch; 11, extrusion block; 12, L-shaped slot; 13, L-shaped block; 14, plug hole; 15, sealing plug; 16, plug slot; 17, threaded rod. DETAILED DESCRIPTION

[0032] The following will be described in detail in combination with the accompanying drawings. Figures 1-4 The present application will be further described in detail.

[0033] The embodiment of the present application discloses a tube rack structure for fluorescent quantitative PCR.

[0034] Referring to Figure 1 , Figure 2 , Figure 3 A tube rack structure for fluorescent quantitative PCR, comprising a bottom frame 1, a plurality of shrinkage holes 5 are formed in the upper end surface of the bottom frame 1, the plurality of shrinkage holes 5 are all through the bottom of the bottom frame 1 and are in communication with the outside, the shrinkage holes 5 are all conical in shape, and the conical shrinkage holes 5 are downward, a movable cavity 6 is formed around the plurality of shrinkage holes 5 in the inside of the bottom frame 1, and a plurality of through holes are formed in the inner wall of the movable cavity 6 at the positions of the shrinkage holes 5, so that the plurality of shrinkage holes 5 are all in communication with the movable cavity 6, and a threaded hole 7 is formed in the center of the upper end surface of the bottom frame 1, the threaded hole 7 is through the bottom frame 1 at the bottom and is in communication with the movable cavity 6, a fixed block 9 is movably arranged in the movable cavity 6, the fixed block 9 is formed by a plurality of rectangular plates, and is inserted into the movable cavity 6 in a fitted manner.

[0035] It should be noted that arc-shaped notches 10 are formed in the side surface of the fixed block 9 at the positions of the plurality of shrinkage holes 5, the width of the arc-shaped notches 10 is smaller than the width of the through holes of the movable cavity 6 and the shrinkage holes 5, an extrusion block 11 is fixedly arranged on the side surface of the fixed block 9 at the position of the threaded hole 7, and the top side of the extrusion block 11 is inclined, in a normal state, the extrusion block 11 is inserted into the threaded hole 7 from the communication part of the movable cavity 6, one end of the side surface of the fixed block 9 is fixedly connected with a plurality of springs 8 (the number of the springs 8 is at least three), and the other end of the springs 8 away from the fixed block 9 is fixedly connected with the inner wall of the movable cavity 6.

[0036] When the extrusion block 11 is subjected to a pushing force and moves into the movable cavity 6 from the threaded hole 7, the fixed block 9 is driven to move as a whole, the arc-shaped notches 10 are driven to move into the shrinkage holes 5 from the movable cavity 6 along with the movement of the fixed block 9, so that the test tubes in the shrinkage holes 5 are extruded, and the bottom of the test tubes is fixed in the shrinkage holes 5, when the extrusion block 11 loses the pushing force, the plurality of springs 8 rebound to drive the fixed block 9 to move in the opposite direction, so that the arc-shaped notches 10 are pushed back into the movable cavity 6, and the extrusion block 11 is pushed back into the threaded hole 7.

[0037] Referring to Figure 1 , Figure 2 , Figure 4, the top frame 2 is provided with a plurality of L-shaped grooves 12, and the L-shaped block 13 is located in the L-shaped groove 12.

[0038] It should be noted that the top frame 2 is movably inserted with the abutting plate 4, the surface of the abutting plate 4 is provided with the insertion slot 16 at the position of the threaded port 3, and the inner wall of the insertion slot 16 is movably inserted with the outer surface of the threaded port 3; the lower end surface of the abutting plate 4 is provided with the sealing plug 15 at the position of the plurality of insertion holes 14; the sealing plug 15 is made of rubber material and has deformation property; in normal state, the inclined surface of the plurality of L-shaped blocks 13 is located in the hollow range of the threaded port 3.

[0039] The threaded rod 17 is rotated into the threaded port 3 to apply pressure to the inclined surface of the plurality of L-shaped blocks 13, so that the L-shaped block 13 protrudes outward along the L-shaped groove 12, so that the L-shaped protruding part of the L-shaped block 13 is located on the top surface of the abutting plate 4, and the L-shaped block 13 is limited by the L-shaped groove 12, so as to apply longitudinal limitation to the abutting plate 4, and fix the abutting plate 4 in the recess of the top frame 2; with the fixation of the abutting plate 4, the plurality of sealing plugs 15 are pressed into the insertion holes 14, and the sealing plugs 15 seal the tube opening of the test tube while applying pressure to the top of the test tube for fixation, so as to prevent the sample from spilling; with the continuous rotation of the threaded rod 17 into the threaded hole 7 to form threaded connection, the top frame 2 and the bottom frame 1 are connected, the stability is improved, and the threaded rod 17 applies a pushing force to the extrusion block 11 to provide a moving power for the extrusion block 11.

[0040] Among them, the device further includes the spring 8, the sealing plug 15, the threaded rod 17 and the detector on one side of the bottom frame 1, which are all prior art, and their structures will not be described in detail, and the plurality of contraction holes 5 and the insertion holes 14 are coated with lubricant, so as to facilitate personnel to collect the test tube and prevent the test tube from being collided.

[0041] The implementation principle of the tube rack structure of the fluorescence quantitative PCR embodiment of the application is as follows: when the tube rack is used, a person inserts a test tube in which a sample is stored into the insertion hole 14 and inserts the bottom of the test tube into the contraction hole 5, thereby completing storage, and then inserts the abutting plate 4 into the groove at the top of the top frame 2, with the insertion of the abutting plate 4, the sealing plug 15 is driven to move downward, and when the abutting plate 4 is inserted into the groove of the top frame 2, the sealing plug 15 is inserted into the mouth of the test tube, thereby forming a sealed state of the test tube.

[0042] Then, the threaded rod 17 is rotated downward along the threaded hole 3, the bottom of the threaded rod 17 first collides with the inclined surface of the plurality of L-shaped blocks 13 and is extruded as the threaded rod 17 continues to rotate in, thereby pushing the L-shaped blocks 13 to move along the L-shaped grooves 12, and finally the protrusions at the bottom of the L-shaped blocks 13 protrude from the openings of the L-shaped grooves 12 (as shown in Figure 2 Fig. 3), thereby limiting the top of the abutting plate 4 and fixing the abutting plate 4 in the groove of the top frame 2 to prevent sliding out.

[0043] The threaded rod 17 continues to rotate to form a connection with the threaded hole 7, thereby improving the overall stability of the bottom frame 1 and the top frame 2, and then the bottom of the threaded rod 17 contacts the inclined surface of the extrusion block 11 to form extrusion, thereby pushing the extrusion block 11 to move along the through hole of the movable cavity 6, and the extrusion block 11 drives the fixed block 9 to move synchronously, thereby causing the arc-shaped notch 10 to be pressed into the contraction hole 5 to generate a transverse pushing force on the bottom of the test tube, thereby causing the test tube to fit the inner wall of the contraction hole 5 to prevent gaps, cooperate with the top of the abutting plate 4 to be fixed, thereby sealingly and fixedly connecting the test tube as a whole between the bottom frame 1 and the top frame 2, preventing the test tube from being damaged by collision due to vibration, and at the same time, a person can observe the test tube label and sample storage condition through the hollow between the bottom frame 1 and the top frame 2 to avoid sample misplacement.

[0044] The above is only an optional embodiment of the present disclosure and is not used to limit the present disclosure. For those skilled in the art, the present disclosure can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present disclosure shall be included in the protection scope of the present disclosure.

Claims

1. A tube rack structure for fluorescent quantitative PCR, characterized by: The invention comprises a bottom frame (1) arranged on one side of the detector, a movable cavity (6) is provided inside the bottom frame (1), a fixing block (9) for fixing a test tube is movably provided in the movable cavity (6), the top of the bottom frame (1) is connected to the top frame (2), and a hollow is formed on the side opposite to the top frame (2) for storing the test tube; A threaded opening (3) is provided at the center of the top of the top frame (2); a clamping plate (4) for sealing the test tube is provided around the threaded opening (3) at the top of the top frame (2); a plurality of L-shaped blocks (13) for fixing the clamping plate (4) are elastically connected in the threaded opening (3); The outer surface of the threaded opening (3) is provided with a plurality of L-shaped grooves (12), the plurality of L-shaped grooves (12) are movably connected to the plurality of L-shaped blocks (13), and the internal thread of the threaded opening (3) is connected to a threaded rod (17); A threaded hole (7) is provided at the center of the upper end surface of the bottom frame (1), and the threaded hole (7) passes through the bottom frame (1) and is in communication with the active cavity (6).

2. The tube rack structure for fluorescent quantitative PCR according to claim 1, characterized in that: The upper end surface of the bottom frame (1) is provided with a plurality of shrinkage holes (5), and the plurality of shrinkage holes (5) all penetrate the bottom frame (1) and communicate with the active cavity (6).

3. The tube rack structure for fluorescent quantitative PCR according to claim 1, characterized in that: The fixing block (9) is provided with arc-shaped notches (10) at the positions of the plurality of shrinkage holes (5), and the fixing block (9) is provided with an extrusion block (11) at the position of the threaded hole (7), and the extrusion block (11) is movably inserted in the threaded hole (7).

4. The tube rack structure for fluorescent quantitative PCR according to claim 1, characterized in that: A plurality of springs (8) are connected to a side of the outer surface of the fixed block (9) close to the inner wall of the movable cavity (6), and one end of the plurality of springs (8) away from the fixed block (9) is fixedly connected to the inner wall of the movable cavity (6).

5. The tube rack structure for fluorescent quantitative PCR according to claim 2, characterized in that: Insertion holes (14) are provided at the bottom of the top frame (2) at the positions of the multiple shrinkage holes (5).

6. The tube rack structure for fluorescent quantitative PCR according to claim 1, characterized in that: A slot (16) is provided in the middle of the abutting plate (4), and the inner wall of the slot (16) is movably plugged into the threaded opening (3). A plurality of sealing plugs (15) are fixedly provided at the positions of the plurality of insertion holes (14) at the bottom of the abutting plate (4).

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