Bacteria sampling ball and bacteria sampler

Abstract

The utility model relates to the technical field of microbiology devices, in particular to a bacterium sampling ball and a bacterium sampler, which comprise a vacuum generator and a sampling assembly, the sampling assembly comprises an air pipe, a connector, a connecting seat, a pen container and an adsorption part, and the pen container is provided with an air hole; when bacterial colonies are sampled, a user holds the pen container and then starts the vacuum generator, negative pressure is formed in the air holes and the adsorption part, the adsorption part is aligned with the sterilized sampling balls, the sampling balls can be adsorbed on the adsorption part, then the sampling balls are moved to the surfaces of the bacterial colonies in the culture dish, and the bacterial colonies are attached to the surfaces of the sampling balls; then, the sampling ball attached with the bacterial colony sample is placed in a sampling container, so that the sampling of the bacterial colony is completed; according to the sampling mode provided by the invention, since the sample does not need to be immersed in the sterile normal saline as a sterile cotton swab for sampling, the reduction of the concentration and purity of the bacterial colony sample can be avoided, and the influence on the subsequent detection result is prevented.

Description

TECHNICAL FIELD

[0001] The utility model relates to microbiology device technical field especially relates to a bacterium sampling ball and bacterium sampler. BACKGROUND

[0002] Colony sampling is a common and important experimental step in microbiology, bioengineering, pharmaceutical and other fields. The main purpose of colony sampling is to select bacterial colonies with specific characteristics or functions from complex microbial mixtures for further research, analysis and application.

[0003] The current bacterial colony sampling method is mostly by the experiment personnel to the colony in the culture dish with sterile cotton swab manual sampling, uses the sterile cotton swab sampling, first immerse it in sterile normal saline, then gently wipe the colony surface to collect the bacterial colony sample, but because the sterile cotton swab is usually made of fiber material, has certain water absorption, in the sampling process, the cotton swab can absorb too much normal saline, thereby dilutes the bacterial colony sample, reduces the concentration and purity of the sample, thereby can influence the subsequent detection result. SUMMARY

[0004] The utility model discloses a bacterium sampling ball and bacterium sampler can sample the bacterial colony, avoid reducing the concentration and purity of the bacterial colony sample, prevent influencing the subsequent detection result.

[0005] To achieve the above object, first, the utility model provides a bacterium sampler, including vacuum generator and sampling assembly, the sampling assembly includes air pipe, connecting head, connecting seat, pen barrel and adsorption part,

[0006] The air pipe and the vacuum generator are fixedly connected and communicated, and are located at the side of the vacuum generator, the connecting head and the air pipe are fixedly connected, and are located at the side of the air pipe, the connecting seat is arranged at the side of the connecting head, the pen barrel is arranged at the side of the connecting seat, the pen barrel has a gas hole, and the gas hole is communicated with the air pipe, and the adsorption part is arranged at the side of the pen barrel and communicated with the gas hole.

[0007] The connecting seat includes a seat body and two clamping pieces, the seat body and the pen barrel are fixedly connected and located at the side of the pen barrel, and the two clamping pieces are mirror image arranged on the two sides of the seat body.

[0008] The clamping piece includes a first spring, a pull block and a lock rod, the first spring and the seat body are fixedly connected and located at the side of the seat body, the pull block and the first spring are fixedly connected and located at the end of the first spring away from the seat body, and the lock rod and the pull block are fixedly connected and penetrate through the first spring and the seat body.

[0009] The adsorption component comprises a hollow tube, an adsorption head, a circular ring and a second spring; the hollow tube is slidably connected with the pen barrel and communicates with the air hole and is located at the side of the pen barrel; the adsorption head is fixedly connected with the hollow tube and communicates with the hollow tube and is located at the end of the hollow tube away from the pen barrel; the circular ring is fixedly connected with the hollow tube and is located at the side of the hollow tube; one end of the second spring is fixedly connected with the circular ring and the other end is fixedly connected with the pen barrel and is located between the circular ring and the pen barrel.

[0010] The utility model discloses a kind of bacteria sampling balls, including sampling sphere, the sampling sphere has multiple micropores, multiple The micropores are evenly distributed on the surface of the sampling sphere.

[0011] The bacteria sampling ball and the bacteria sampler of the utility model, the bacteria sampler of the present application needs to be equipped with the medium of adhering bacterial colony to use, the medium selects sampling ball, the sampling ball is spherical, the material of the sampling ball can be any one of stainless steel, glass, ceramic, plastic etc.;When sampling bacterial colony, the user holds the pen barrel and starts the vacuum generator, the air hole and the adsorption component inside form negative pressure, the adsorption component is aligned with the sampling ball after sterilization, the sampling ball is adsorbed on the adsorption component, then the sampling ball is moved to the surface of bacterial colony in petri dish, the bacterial colony is attached to the surface of the sampling ball by contacting with the sampling ball, then the sampling ball with bacterial colony sample is moved to sampling container, the vacuum generator is closed, the sampling ball falls in the sampling container, so as to complete the sampling of bacterial colony;The sampling mode of the present application, because it does not need to be immersed in sterile physiological saline as the same as sampling with sterile cotton swab, can avoid reducing the concentration and purity of bacterial colony sample, prevent affecting subsequent detection results. BRIEF DESCRIPTION OF DRAWINGS

[0012] In order to more clearly illustrate the technical scheme in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiment or the prior art description will be briefly introduced as follows.

[0013] Figure 1 It is a structure schematic view of the bacteria sampler of the utility model.

[0014] Figure 2 It is a sectional view of the bacteria sampler of the utility model.

[0015] Figure 3 It is a structure schematic view of the bacteria sampling ball of the utility model.

[0016] Figure 4 It is a structure schematic view of the bacteria sampling ball of the utility model applied to the bacteria sampler.

[0017] 101-vacuum generator, 102-air pipe, 103-connector, 104-connector seat, 105-pen barrel, 106-adsorption component, 107-air hole, 108-seat body, 109-clamping piece, 110-first spring, 111-pull block, 112-locking rod, 113-hollow pipe, 114-adsorption head, 115-ring, 116-second spring, 201-sampling sphere, 202-micropore. DETAILED DESCRIPTION

[0018] The embodiments of the present application are described in detail below, examples of which are shown in the drawings, the embodiments described below by referring to the drawings are exemplary, and are intended to explain the present application, and cannot be understood as a limitation of the present application.

[0019] In a first aspect, refer to Figures 1-2 , wherein, Figure 1 is a structural diagram of a bacteria sampler of the present application, Figure 2 is a sectional view of a bacteria sampler of the present application.

[0020] The present application provides a kind of bacteria sampler, including vacuum generator 101 and sampling assembly, the sampling assembly includes air pipe 102, connector 103, connector seat 104, pen barrel 105 and adsorption component 106;The connector seat 104 includes seat body 108 and two clamping pieces 109;The clamping piece 109 includes first spring 110, pull block 111 and locking rod 112;The adsorption component 106 includes hollow pipe 113, adsorption head 114, ring 115 and second spring 116;By the foregoing scheme, bacteria colony can be sampled, avoid reducing the concentration and purity of bacteria colony sample, prevent affecting subsequent detection result.

[0021] For the specific embodiment, the air pipe 102 and the vacuum generator 101 are fixedly connected and communicated, and located at the side of the vacuum generator 101; the connecting head 103 and the air pipe 102 are fixedly connected, and located at the side of the air pipe 102; the connecting seat 104 is arranged at the side of the connecting head 103; the pen barrel 105 is arranged at the side of the connecting seat 104, and the pen barrel 105 has an air hole 107, which is communicated with the air pipe 102; the adsorption component 106 is arranged at the side of the pen barrel 105, and communicated with the air hole 107. The bacterial sampler of the application needs to be equipped with a medium for adhering bacterial colonies for use. In the embodiment, the medium is a sampling ball. The sampling ball is spherical, and the material of the sampling ball can be any one of stainless steel, glass, ceramic, plastic and the like. When sampling the bacterial colonies, the user holds the pen barrel 105 and starts the vacuum generator 101, a negative pressure is formed inside the air hole 107 and the adsorption component 106, the adsorption component 106 is aligned with the sterilized sampling ball, the sampling ball is adsorbed on the adsorption component 106, then the sampling ball is moved to the surface of the bacterial colonies in the culture dish, the colonies are attached to the surface of the sampling ball by contacting the sampling ball, then the sampling ball with the bacterial colony sample is moved to the sampling container, the vacuum generator 101 is closed, and the sampling ball falls into the sampling container, thereby completing the sampling of the bacterial colonies. The sampling method of the application can avoid reducing the concentration and purity of the bacterial colony sample, and prevent affecting the subsequent detection results, because it does not need to be immersed in sterile saline as when sampling with a sterile cotton swab.

[0022] The seat body 108 and the pen barrel 105 are fixedly connected and located at the side of the pen barrel 105; two clamping pieces 109 are mirror image arranged on both sides of the seat body 108. The seat body 108 is used to limit the connecting head 103, and the position of the connecting head 103 can be fixed by the two clamping pieces 109, so as to connect the air pipe 102 and the pen barrel 105.

[0023] Secondly, the first spring 110 is fixedly connected with the seat body 108 and located at the side of the seat body 108; the pull block 111 is fixedly connected with the first spring 110 and located at the end of the first spring 110 away from the seat body 108; the lock rod 112 is fixedly connected with the pull block 111 and penetrates through the first spring 110 and the seat body 108. The connecting head 103 is provided with placing grooves on two sides thereof which are adapted to the lock rod 112. When the air pipe 102 and the pen barrel 105 are connected, the pull block 111 is first pulled to make the lock rod 112 away from the seat body 108, at this time, the first spring 110 is stretched, and then the connecting head 103 is placed in the seat body 108, and then the pull block 111 is released, the lock rod 112 is inserted into the placing grooves on the connecting head 103 under the driving of the first spring 110, the connecting head 103 is fixed in the seat body 108 by the two lock rods 112, so that the air pipe 102 and the pen barrel 105 are connected. When the air pipe 102 needs to be disassembled, the pull block 111 is pulled to make the lock rod 112 away from the connecting head 103, so that the fixing of the connecting head 103 is released, and then the air pipe 102 can be disassembled. Through the fixing mode, the air pipe 102 can be conveniently installed and disassembled.

[0024] Meanwhile, the hollow pipe 113 is slidably connected with the pen barrel 105, communicates with the air hole 107 and is located at the side of the pen barrel 105; the adsorption head 114 is fixedly connected with the hollow pipe 113 and communicates with the hollow pipe 113 and is located at the end of the hollow pipe 113 away from the pen barrel 105; the circular ring 115 is fixedly connected with the hollow pipe 113 and located at the side of the hollow pipe 113; one end of the second spring 116 is fixedly connected with the circular ring 115 and the other end is fixedly connected with the pen barrel 105 and located between the circular ring 115 and the pen barrel 105. When the sampling ball is adsorbed, the adsorption head 114 contacts with the sampling ball, and the second spring 116 can provide a buffer distance to prevent the adsorption head 114 and the sampling ball from being damaged due to excessive force when the adsorption head 114 contacts with the sampling ball.

[0025] When the bacteria sampler is used to sample the bacterial colonies, the user holds the pen barrel 105 and then starts the vacuum generator 101, the air hole 107, the hollow tube 113 and the adsorption head 114 form a negative pressure inside, the adsorption head 114 is aligned with the sampling ball after sterilization, and the sampling ball is adsorbed on the adsorption head 114, when the sampling ball is adsorbed, the adsorption head 114 contacts the sampling ball, a buffer distance is provided by the second spring 116, so that the adsorption head 114 and the sampling ball are prevented from being damaged due to excessive force when the adsorption head 114 contacts the sampling ball. Then, the sampling ball is moved to the surface of the bacterial colonies in the culture dish, the colonies contact the sampling ball and are attached to the surface of the sampling ball, then the sampling ball with the colony sample is moved to the sampling container, the vacuum generator 101 is closed, and the sampling ball falls into the sampling container, so that the sampling of the bacterial colonies is completed; the sampling method of the application can avoid reducing the concentration and purity of the bacterial colony sample, and prevent affecting the subsequent detection result, because it does not need to be immersed in sterile saline as when sampling with a sterile cotton swab.

[0026] In a second aspect, referring to Figures 3-4 , wherein, Figure 3 is a structural schematic view of a bacteria sampling ball of the utility model, Figure 4 is a structural schematic view of a bacteria sampling ball applied to a bacteria sampler.

[0027] The utility model further provides a bacteria sampling ball, including sampling ball body 201, sampling ball body 201 has multiple micropores 202, multiple micropores 202 are evenly distributed on the surface of sampling ball body 201.

[0028] For the specific embodiment, the sampling ball body 201 is used with the bacteria sampler, and the multiple micropores 202 arranged on the surface of the sampling ball body 201 can increase the attachment capacity, provide more attachment points for bacteria, so that the bacterial colonies are attached to the sampling ball body 201. The material of the sampling ball body 201 can be any one of stainless steel, glass, ceramic, plastic and the like.

[0029] When the bacteria sampling ball is used for sampling bacteria colonies, the user holds the pen barrel 105, turns on the vacuum generator 101, negative pressure is formed in the air hole 107, the hollow tube 113 and the adsorption head 114, the adsorption head 114 is aligned with the sterilized sampling ball body 201, the sampling ball body 201 is adsorbed on the adsorption head 114, then the sampling ball body 201 is moved to the surface of the bacteria colonies in the culture dish, the colonies are in contact with the sampling ball body 201 and are attached to the micropores 202 on the surface of the sampling ball body 201, then the sampling ball body 201 with the colony sample is moved to the sampling container, the vacuum generator 101 is turned off, and the sampling ball body 201 falls into the sampling container, so that the sampling of the bacteria colonies is completed.

[0030] The above disclosure is only one or more preferred embodiments of the application, and cannot limit the scope of the application. Those skilled in the art can understand that all or part of the above-mentioned embodiments can be implemented, and equivalent changes made according to the claims of the application still belong to the scope covered by the application.

Claims

1. A bacterial sampler, comprising a vacuum generator, characterized in that: Also included are sampling components; The sampling assembly includes an air tube, a connector, a connector seat, a pen holder and an adsorption component; The air pipe is fixedly connected and communicated with the vacuum generator and is located on the side of the vacuum generator; the connecting head is fixedly connected with the air pipe and is located on the side of the air pipe; the connecting seat is arranged on the side of the connecting head; the pen holder is arranged on the side of the connecting seat, and the pen holder has an air hole, which is communicated with the air pipe; the adsorption component is arranged on the side of the pen holder and is communicated with the air hole.

2. A bacteria sampler as claimed in claim 1, characterized in that: The connecting seat includes a seat body and two clamping members; the seat body is fixedly connected to the pen holder and is located on the side of the pen holder; the two clamping members are mirror-imaged and arranged on both sides of the seat body.

3. A bacteria sampler as claimed in claim 2, characterized in that: The clamping member includes a first spring, a pulling block and a locking rod; the first spring is fixedly connected to the base body and is located on the side of the base body; the pulling block is fixedly connected to the first spring and is located at an end of the first spring away from the base body; the locking rod is fixedly connected to the pulling block and passes through the first spring and the base body.

4. A bacteria sampler as claimed in claim 3, characterized in that: The adsorption component includes a hollow tube, an adsorption head, a circular ring, and a second spring; the hollow tube is slidably connected to the pen barrel, communicates with the air hole, and is located on the side of the pen barrel; the adsorption head is fixedly connected and communicated with the hollow tube, and is located at one end of the hollow tube away from the pen barrel; the circular ring is fixedly connected to the hollow tube and is located on the side of the hollow tube; One end of the second spring is fixedly connected to the circular ring, and the other end is fixedly connected to the pen holder, and is located between the circular ring and the pen holder.

5. A bacteria sampling ball, used in a bacteria sampler according to any one of claims 1 to 4, characterized in that: The sampling sphere comprises a sampling sphere having a plurality of micropores, and the plurality of micropores are uniformly distributed on the surface of the sampling sphere.

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