Accurate pusher for protein filler preassembled column

Abstract

The utility model discloses an accurate pusher for a protein filler preassembled column. The accurate pusher comprises an inner cylinder, an outer cylinder and an inner rod, the inner cylinder and the outer cylinder are both of a straight cylinder structure, the outer cylinder is concentrically and externally embedded outside the inner cylinder and is in sliding and rotating fit with the inner cylinder, an inner rod of a variable-diameter round body structure is fixed to the center of the outer cylinder, a piston is arranged at the top of the inner rod, and a piston pipe of a pipeline structure is fixedly embedded into the end, close to the piston, of the inner cylinder. The piston is embedded in the piston tube and forms piston fit with the piston tube, a screw tube is externally embedded and fixed on the outer circular surface of the inner rod, a spiral external thread is arranged on the screw tube, an inner wall thread in threaded fit with the external thread is arranged on the inner wall of the inner cylinder, and straight carving parts distributed in the axial direction of the inner cylinder are arranged on the outer wall of the inner cylinder; the material pushing device has the beneficial effect of high material pushing precision.

Description

TECHNICAL FIELD

[0001] The utility model belongs to protein purification equipment technical field, especially relate to a kind of accurate pusher for protein filler pre-packed column. BACKGROUND

[0002] The patent document with the publication number CN203091244U mentions that in the field of life sciences, fillers are often used to separate and purify proteins. When using fillers, they are divided into chromatography methods and batch adsorption methods depending on whether a chromatography column is used. The former is more widely used. When using chromatography methods, the filler needs to be evenly loaded into the column to prepare a pre-packed chromatography column. When the mixed protein solution passes through the chromatography column at a certain flow rate, it is selectively adsorbed with the filler.

[0003] The existing pusher for protein filler pre-packed column mostly uses a syringe for pusher operation. However, when using a syringe for pusher operation, it is difficult to accurately control the pusher volume. Some experiments require high accuracy in protein liquid volume, and an auxiliary accurate measuring device is needed to take the volume, which is time-consuming and labor-intensive. Therefore, the existing technology needs an accurate pusher for protein filler pre-packed column. UTILITY MODEL CONTENT

[0004] The utility model aims to provide an accurate pusher for protein filler pre-packed column to solve the technical problems mentioned in the background.

[0005] The above technical purpose of the utility model is achieved through the following technical solutions:

[0006] An accurate pusher for protein filler pre-packed column includes an inner cylinder, an outer cylinder, and an inner rod. The inner cylinder and the outer cylinder are straight cylinder structures. The outer cylinder is concentrically embedded outside the inner cylinder and is in sliding and rotating cooperation with the inner cylinder. The center of the outer cylinder is fixed with a variable-diameter cylindrical structure of the inner rod. The top of the inner rod is provided with a piston. The inner cylinder is embedded with a pipe structure of a piston pipe at one end near the piston. The piston is embedded in the piston pipe and forms a piston cooperation with the piston pipe. The outer cylindrical surface of the inner rod is externally embedded with a spiral pipe. The spiral pipe has helical external threads. The inner wall of the inner cylinder is provided with inner wall threads that are in threaded cooperation with the external threads. The outer wall of the inner cylinder is provided with a straight scale distributed along the axial direction of the inner cylinder.

[0007] Further, to further improve the pusher accuracy, the outer cylinder is provided with an annular scale at one end near the inner cylinder. The annular scale is a scale line that evenly divides the outer cylindrical wall of the outer cylinder into multiple small scales. The straight scale is provided with an indicating line parallel to the axial line of the inner cylinder for indicating the annular scale.

[0008] Further, in order to facilitate the connection of the protein filler preloaded column, the end face of the end of the inner cylinder away from the outer cylinder is provided with an inner recessed annular structure to form a connecting ring groove, and the outer wall of the connecting ring groove is provided with a helical internal thread.

[0009] Further, in order to improve the concentricity of the screw pipe and the inner rod, and further improve the operation accuracy of the pushing, the screw pipe is not distributed with external threads on a part of one end, and the part is divided by circumferentially distributed division gaps, the end of the screw pipe is divided into a plurality of petal-shaped structures of the clamping petals, the end of the clamping petals is treated with an outer ring chamfer, the screw pipe is integrally embedded on the inner rod, and an annular outer hoop is clamped tightly on all the clamping petals, so that the clamping petals are tightly contracted to concentrically clamp and fix the inner rod.

[0010] Further, in order to improve the connection strength and stability of the outer cylinder and the inner rod, the connecting parts of the outer hoop and the screw pipe are provided with concentric and same-diameter threaded holes, and a second locking bolt penetrates the outer wall of the outer cylinder and is screwed in the two groups of threaded holes to lock and fix the outer cylinder, the screw pipe, the outer hoop and the inner rod.

[0011] Further, in order to further improve the connection strength of the outer cylinder and the inner rod, the same side end of the inner rod is concentrically wrapped at the end of the outer cylinder away from the piston, and a first locking bolt penetrates the outer wall of the outer cylinder to lock the inner rod in the outer cylinder.

[0012] Further, in order to facilitate the fixation of the inner cylinder during use and facilitate the reading of the straight scale on the inner cylinder, the inner cylinder is embedded with two groups of pinching columns of columnar structures with axes perpendicular to the axis of the inner cylinder, and the two groups of pinching columns are symmetrical and concentric structures.

[0013] Further, in order to facilitate the pinching of the fingers of the person and avoid slipping when pinching, the outer end face of the pinching column is provided with an inner recessed arc-shaped groove, and the arc-shaped inner wall of the arc-shaped groove is provided with anti-skid lines or anti-skid pasting layers.

[0014] In summary, the present application has the following advantages:

[0015] The pushing precision is high: through the establishment of the straight scale and the ring scale, the outer cylinder is slowly rotated, the inner rod connected with the outer cylinder is rotated, the screw pipe fixed on the inner rod is synchronously rotated, because the screw pipe and the inner cylinder are threadedly matched through the inner wall threads, so under the rotation holding of the screw pipe, the screw pipe will drive the outer cylinder and the inner rod connected therewith to move along the axial direction of the inner cylinder, according to the above principle, the rotation direction of the outer cylinder can be controlled to make the outer cylinder move towards the direction of the connecting ring groove, and the corresponding indication number of the lower end face of the outer cylinder on the straight scale changes during the movement, thereby the injection gas amount of the protein filler preloaded column can be controlled and the liquid outlet precision of the protein filler preloaded column can be controlled; the ring scale is divided into a plurality of small scale lines by equally dividing the outer circumferential wall of the outer cylinder, and more accurate pushing precision can be obtained by indicating the scale lines on the ring scale through the indicating line. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 is the overall structure schematic diagram of the utility model;

[0017] Figure 2 is the explosion structure schematic diagram of the utility model;

[0018] Figure 3 is the overhead structure schematic diagram of the utility model;

[0019] Figure 4 is Figure 3 A-A section structure schematic diagram in the utility model;

[0020] Figure 5 is the assembly structure schematic diagram of the screw pipe in the utility model;

[0021] Figure 6 is the use structure schematic diagram of the utility model with the preloaded column.

[0022] In the figure, 1, inner cylinder;2, outer cylinder;3, inner rod;4, straight scale;5, ring scale;6, kneading column;7, screw pipe;8, piston pipe;9, piston;10, protein filler preloaded column;11, inner wall thread;12, connecting ring groove;31, first locking bolt;41, indicating line;61, arc-shaped groove;71, outer hoop ring;72, second locking bolt;73, segmentation gap;74, clamping piece;75, external thread. DETAILED DESCRIPTION

[0023] The utility model will be further explained in detail in combination with the drawings.

[0024] Embodiment:

[0025] Please refer to Figures 1-6 The utility model provides technical scheme:

[0026] A kind of accurate pusher for protein filler preloaded column, including: inner cylinder 1, outer cylinder 2, inner rod 3;The inner cylinder 1, outer cylinder 2 are straight cylinder structure, outer cylinder 2 concentrically embed in inner cylinder 1 outer and inner cylinder 1 keep sliding and rotation cooperation, the center of outer cylinder 2 is fixed with the inner rod 3 of the variable-diameter round body structure, the top of inner rod 3 is provided with a piston 9, inner cylinder 1 is embedded in one end close to piston 9 with the pipeline structure of piston pipe 8, piston 9 is embedded in piston pipe 8 and piston pipe 8 forms piston cooperation, inner rod 3 outer circumferential surface is embedded with a screw pipe 7, screw pipe 7 is respectively provided with helical external thread 75, the inner wall of inner cylinder 1 is provided with the inner wall thread 11 of screw thread cooperation with external thread 75, the outer wall of inner cylinder 1 is provided with straight scale 4 along the axial direction distribution of inner cylinder 1.

[0027] The outer cylinder 2 is provided with an annular ring scale 5 at one end close to the inner cylinder 1. The ring scale 5 is a scale line that evenly divides the outer circumferential wall of the outer cylinder 2 into multiple small scales; the straight scale 4 is provided with an indicator line 41 parallel to the axis of the inner cylinder 1 for indicating the ring scale 5.

[0028] The end surface of the inner cylinder 1 away from the outer cylinder 2 is provided with an inwardly concave annular structure to form a connecting ring groove 12, and the outer wall of the connecting ring groove 12 is provided with a spiral internal thread.

[0029] The spiral tube 7 has a portion at one end without external threads 75, and this portion is divided by circumferentially distributed dividing gaps 73, dividing the end of the spiral tube 7 into multiple petals 74 of a petal-shaped structure. The outer ring of the end of the petal 74 is chamfered, and the spiral tube 7 is embedded in the inner rod 3 as a whole. An outer hoop 71 with an annular structure is used to clamp all the petals 74, so that the petals 74 are tightened to concentrically clamp the inner rod 3.

[0030] The connecting parts of the outer hoop 71 and the screw tube 7 are both provided with concentric and same-diameter threaded holes. The second locking bolt 72 is used to penetrate the outer wall of the outer tube 2 and is screwed into the above two sets of threaded holes to lock and fix the outer tube 2, the screw tube 7, the outer hoop 71 and the inner rod 3.

[0031] The end of the outer tube 2 away from the piston 9 concentrically wraps the same side end of the inner rod 3, and the first locking bolt 31 penetrates the outer wall of the outer tube 2 to lock the inner rod 3 in the outer tube 2.

[0032] The inner cylinder 1 is embedded with two groups of kneading columns 6 of cylindrical structure whose axis lines are perpendicular to the axis line of the inner cylinder 1 , and the two groups of kneading columns 6 are symmetrical and concentric structures.

[0033] The outer end surface of the kneading column 6 is provided with an inwardly concave arc-shaped groove 61 , and the arc-shaped inner wall of the arc-shaped groove 61 is provided with anti-slip lines or an anti-slip layer.

[0034] Brief description of the usage process:

[0035] When using, such as Figure 6 As shown, the air inlet connector of the protein filler pre-loaded column 10 is screwed and connected in the connecting ring groove 12, and the liquid outlet of the protein filler pre-loaded column is placed downward. The arc-shaped grooves 61 of the two kneading columns 6 are kneaded with the thumb and index finger of the left hand respectively, thereby fixing the inner cylinder 1 and the kneading columns 6 as a whole.

[0036] The outer cylinder 2 is rotated again at a low speed, the outer cylinder 2 drives the inner rod 3 connected therewith to rotate, the inner rod 3 drives the screw pipe 7 fixed thereon to rotate synchronously, because the screw pipe 7 is screwed with the inner cylinder 1 through the inner wall screw thread 11, under the rotation of the screw pipe 7, the screw pipe 7 drives the outer cylinder 2 and the inner rod 3 connected therewith to move along the axial direction of the inner cylinder 1, according to the above principle, the rotation direction of the outer cylinder 2 is controlled to drive the outer cylinder 2 to move towards the connecting ring groove 12, the piston 9 at the end of the inner rod 3 moves in the piston pipe 8 along with the inner rod 3, and the gas in the piston pipe 8 is pushed into the protein filler preloaded column 10 as volume exchange, and the protein filler preloaded column 10 starts to discharge liquid; during the movement, the corresponding indication number of the lower end surface of the outer cylinder 2 on the straight scale 4 changes, and then the injection amount of gas to the protein filler preloaded column 10 can be controlled.

[0037] According to the change of the injection amount of gas, the discharge amount of the protein liquid from the protein filler preloaded column 10 can be known, and the accurate pushing of the protein filler preloaded column 10 can be achieved (the protein filler preloaded column 10 is an external device, and the structural principle is not in the protection range of the application, so it is omitted, and the specific structure and use principle can be found in the same batch of patent applications of the application, and the name is: a protein filler preloaded column carrier).

[0038] The ring scale 5 is a scale line for dividing the outer circumferential wall of the outer cylinder 2 into a plurality of small scales, for example, if the outer cylinder 2 rotates one circle, the indication number of the straight scale 4 changes by one scale, and if one scale on the straight scale 4 represents 0.1ml, if the ring scale 5 divides the outer circumferential wall of the outer cylinder 2 into 50 scales, then each scale on the ring scale 5 represents 0.1 / 50=0.002ml, and more accurate pushing precision can be obtained by indicating the scale line on the ring scale 5 through the indicating line 41, so that the pushing precision of the protein filler preloaded column can be further improved through the establishment of the ring scale 5.

[0039] The specific embodiment is only an explanation of the application, and is not a limitation of the application, and those skilled in the art can make modifications without creative contribution according to the needs after reading the specification, but as long as it is within the scope of the claims of the application, it is protected by the patent law.

Claims

1. A precision pusher for a protein filler pre-packed column, comprising: The utility model provides a kind of inner tube (1), outer tube (2), inner rod (3);Its characterized in that: the inner tube (1), outer tube (2) are straight cylinder structure, outer tube (2) concentrically embed in the outer of inner tube (1) and inner tube (1) keep sliding and rotating cooperation, the center of outer tube (2) is fixed with the inner rod (3) of the variable-diameter round body structure, the top of inner rod (3) is equipped with a piston (9), inner tube (1) is embedded in one end close to piston (9) with the pipe structure of piston pipe (8), piston (9) is embedded in piston pipe (8) and piston pipe (8) forms piston cooperation, the outer surface of inner rod (3) is embedded with a spiral pipe (7), spiral pipe (7) is respectively provided with helical external thread (75), the inner wall of inner tube (1) is provided with inner wall thread (11) and is threadedly cooperated with external thread (75), the outer wall of inner tube (1) is provided with straight scale (4) distributed along the axial direction of inner tube (1).

2. A precision pusher for pre-packed columns of proteinaceous packing material according to claim 1, characterized in that: The end of outer tube (2) close to inner tube (1) is provided with annular ring scale (5), and the ring scale (5) is a scale line for dividing the outer circumferential wall of the outer tube (2) into a plurality of small scales evenly.

3. The precision pusher for protein packing pre-packed columns of claim 1, wherein: The end surface of the inner tube (1) away from the outer tube (2) is provided with an annular structure of inner recess to form a connecting ring groove (12), and the outer wall of the connecting ring groove (12) is provided with a helical internal thread.

4. The precision pusher for protein packing pre-packed columns of claim 1, wherein: The end portion of the spiral pipe (7) is not distributed with external thread (75), and the end portion is divided into a plurality of petal-shaped clamping petals (74) by using circumferentially distributed division gaps (73), and the end portion of the clamping petals (74) is treated with outer ring chamfering.

5. The precision pusher for protein packing pre-packed columns of claim 4, wherein: The connecting portions of the outer hoop ring (71) and the spiral pipe (7) are provided with concentric and same-diameter threaded holes, a second locking bolt (72) penetrates the outer wall of the outer tube (2) and is screwed in the two groups of threaded holes to lock and fix the outer tube (2), the spiral pipe (7), the outer hoop ring (71) and the inner rod (3).

6. A precision pusher for pre-packed columns of proteinaceous packing material according to claim 5, characterized in that: The same side end of the inner rod (3) is wrapped concentrically at the end of the outer tube (2) away from the piston (9), and a first locking bolt (31) penetrates the outer wall of the outer tube (2) to lock the inner rod (3) in the outer tube (2).

7. The precision pusher for pre-packed columns of proteinaceous fillers according to claim 1, characterized in that: The inner tube (1) is embedded with two groups of cylindrical structures of kneading columns (6) whose axial lines are perpendicular to the axial line of the inner tube (1), and the two groups of kneading columns (6) are symmetrical and concentric structures.

8. The precision pusher for protein packing pre-packed columns according to claim 7, characterized in that: The outer end surface of the kneading column (6) is provided with an arc-shaped recess (61) of inner recess, and the arc-shaped inner wall of the arc-shaped recess (61) is provided with anti-skid lines or anti-skid pasting layers.

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