Non-contact dry type ultrasonic pulverizer

Abstract

The utility model discloses a non-contact dry-type ultrasonic crusher, which comprises a sample disc, a pressing seat and an ultrasonic transducer, a plurality of sample holes for placing sample tubes are arranged on the sample disc, the sample disc is placed on the pressing seat, the ultrasonic transducer is fixed on an installation platform, an ultrasonic tool head is connected onto the ultrasonic transducer, and the ultrasonic tool head is connected with the pressing seat. The pressing seat is arranged on the radial outer side of the ultrasonic tool head in a surrounding mode, an annular cavity is defined by the radial outer surface of the ultrasonic tool head and the radial inner surface of the pressing seat, the annular cavity is used for being in contact fit with the tube wall of a sample tube to achieve positioning of the sample tube, and the sample tube is obliquely inserted into the annular cavity. According to the grinder, the ultrasonic tool head vibrates and transmits energy to perform dry ultrasonic grinding on a sample, so that the sample can be effectively prevented from being polluted, the energy conversion efficiency is high, the grinder is particularly suitable for synchronous ultrasonic treatment of a plurality of nucleic acid samples, and the efficient treatment of the plurality of nucleic acid samples can be generally completed within 1 minute; the nucleic acid extraction efficiency can be greatly improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to a sample pretreatment instrument in the field of biological medicine, in particular to a non-contact dry ultrasonic grinder, which can be applied to nucleic acid extraction. BACKGROUND

[0002] At present, the common nucleic acid extraction methods mainly include magnetic bead method and lysis solution method. The magnetic bead method has the disadvantages of high cost, low extraction efficiency for specific sample types rich in polysaccharides or polyphenols, strong dependence on equipment, and the need for specific auxiliary equipment such as a magnetic stand, which limits its application flexibility in environments lacking such equipment. In addition, the magnetic bead method also has the potential problem of non-specific adsorption. In some cases, the magnetic beads may exhibit non-specific adsorption, affecting the purity of the nucleic acid sample. The lysis solution method is a relatively traditional nucleic acid extraction means, which also has certain limitations in practice. For example, this method relies more on manual operation, so human errors are easily introduced during sample processing, reducing the consistency and reproducibility of experimental results, limiting the sample purification effect, and not suitable for automated processing of large-scale samples. Some lysis solution methods use vortex shakers to shake the samples, but their operation is also affected by the operator's habits, and the shaking time is more than 30 seconds, and after shaking, the samples need to be left to stand for 1-5 minutes, so the overall extraction efficiency is also relatively low.

[0003] An ultrasonic grinder is an analytical instrument widely used in the fields of biology, materials, pharmaceuticals, and chemical industry. The ultrasonic grinder uses the effect of ultrasonic energy aggregation to produce "cavitation" in the liquid, which can break, crush, lyse, and extract cell materials, helping researchers to extract useful components for related research. The common ultrasonic grinders currently available include probe-type ultrasonic grinders and water bath-type non-contact ultrasonic grinders. The probe-type ultrasonic grinders have the risk of cross-contamination of different batches of samples, and the water bath-type non-contact ultrasonic grinders need to use water as the ultrasonic transmission medium, resulting in low ultrasonic energy conversion efficiency. SUMMARY

[0004] The technical problem to be solved by the utility model is to provide a non-contact dry ultrasonic grinder to effectively avoid sample contamination and improve the conversion efficiency of ultrasonic energy, especially for simultaneous ultrasonic processing of multiple nucleic acid samples, which can complete efficient processing of multiple nucleic acid samples within 1 minute, greatly improving the nucleic acid extraction efficiency.

[0005] The utility model discloses a technical scheme that solves the above technical problems: a non-contact dry type ultrasonic wave disintegrator, including sample disc, compression seat and ultrasonic transducer, the sample disc is equipped with a plurality of sample holes for placing sample tube, the sample disc is placed on the compression seat, the ultrasonic transducer is fixed on an installation platform, the ultrasonic transducer is connected with ultrasonic wave tool head, the compression seat is arranged around the radial outer side of ultrasonic wave tool head, the radial outer surface of ultrasonic wave tool head and the radial inner surface of compression seat enclose ring cavity, the ring cavity is used for contacting and cooperating with the pipe wall of sample tube to realize the positioning of sample tube, and the sample tube is obliquely inserted into the ring cavity.

[0006] The utility model discloses a non-contact dry type ultrasonic wave disintegrator, which is used when the sample tube containing the sample is inserted into the sample hole on the sample disc, and the sample tube is obliquely inserted into the ring cavity, so that the pipe wall of the sample tube contacts and cooperates with the ring cavity. The sample tube and the ring cavity have a large contact area. At this time, the ultrasonic transducer can be started to make the ultrasonic wave tool head vibrate and transmit ultrasonic wave energy to the sample tube. The sample is dry ultrasonic wave disintegrated. The sample can be effectively prevented from being contaminated. The conversion efficiency of ultrasonic wave energy is high. It is especially suitable for synchronous ultrasonic wave processing of multiple nucleic acid samples. The efficient processing of multiple nucleic acid samples can be completed within 1 minute. It helps to greatly improve the nucleic acid extraction efficiency.

[0007] As a preferred, the ultrasonic wave tool head is discoid, the outer peripheral wall of ultrasonic wave tool head is equipped with a plurality of first positioning grooves that are up and down through and the cross section is circular arc, the inner peripheral wall of compression seat is equipped with a plurality of second positioning grooves that are up and down through and the cross section is circular arc, the center line of each first positioning groove and each second positioning groove is inclined to the radial inner side of ultrasonic wave tool head from top to bottom respectively, the diameter of each first positioning groove and each second positioning groove is adapted to the outer diameter of sample tube respectively, the ring cavity is enclosed by a plurality of first positioning grooves and a plurality of second positioning grooves, the lower side of installation platform is provided with motor, the motor is used for driving the installation platform to move up and down, so that the ultrasonic wave tool head compresses the pipe wall of sample tube. The motor drives the installation platform and the ultrasonic wave tool head to move synchronously. The ultrasonic wave tool head can be moved to the appropriate position. The ultrasonic wave tool head more tightly and reliably compresses the pipe wall of the sample tube. The ultrasonic wave disintegration effect of the sample is ensured.

[0008] As preferred, the motor is installed on a mounting plate, the top of the mounting plate is vertically fixed with a plurality of guide rods, the upper ends of the guide rods are respectively fixed to the pressing seats, a plurality of guide sleeves are fixed on the mounting platform, each guide sleeve is slidably sleeved on a guide rod, the bottom of the mounting platform is vertically fixed with a plurality of supporting rods, the lower ends of the supporting rods are respectively fixed on a connecting plate, nuts are fixed on the connecting plate, the output end of the motor is connected with a lead screw, and the lead screw is in threaded connection with the nuts. When the motor works, the power of the motor is transmitted to the connecting plate through the lead screw and the nuts, so that the connecting plate, the supporting rods, the mounting platform and the ultrasonic transducer are driven to stably descend or ascend along the guide rods, and the clamping or loosening of the ultrasonic tool head on the sample tube is realized.

[0009] As preferred, the diameters of the first positioning grooves are not completely same, and the diameters of the second positioning grooves are not completely same, so as to meet the placement needs of sample tubes with different outer diameters on the sample disc.

[0010] As preferred, a flange is installed on the mounting platform, the ultrasonic transducer is fixed to the flange, and the ultrasonic tool head is fixed to the upper end of the ultrasonic transducer.

[0011] As preferred, the middle part of the ultrasonic tool head is integrally provided with a first protruding part protruding upward and a second protruding part protruding downward. The first protruding part and the second protruding part can make the vibration and energy transmission of the ultrasonic tool head more uniform.

[0012] Compared with the prior art, the non-contact dry ultrasonic crusher has the following advantages: when the non-contact dry ultrasonic crusher works, the ultrasonic tool head vibrates and transmits ultrasonic energy to the sample tube, the sample is dry ultrasonic crushed, the sample can be effectively prevented from being polluted, the sample tube obliquely inserted into the ring cavity has a large contact area with the ring cavity, the conversion efficiency of ultrasonic energy is high, the non-contact dry ultrasonic crusher is especially suitable for synchronous ultrasonic processing of multiple nucleic acid samples, and efficient processing of the multiple nucleic acid samples can be completed within 1 minute, which helps to greatly improve the nucleic acid extraction efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0013] Figure 1 Effect diagram of the non-contact dry ultrasonic crusher after installing sample tubes with two kinds of outer diameters in the embodiments;

[0014] Figure 2 Corresponding to the longitudinal sectional view of Figure 1 ;

[0015] Figure 3 Pictorial view of the non-contact dry ultrasonic crusher after removing the sample disc;

[0016] Figure 4 For Figure 3 A-A sectional view in the embodiment;

[0017] Figure 5 For the appearance of the ultrasonic transducer and ultrasonic tool head assembly in the embodiment;

[0018] Figure 6 For the longitudinal sectional view corresponding to Figure 5 ;

[0019] Figure 7 For the appearance of the pressing seat in the embodiment;

[0020] The specific reference signs in the drawings are as follows:

[0021] 1-sample disc, 11-sample hole, 2-pressing seat, 21-second positioning groove, 3-ultrasonic transducer, 31-ultrasonic tool head, 32-first positioning groove, 33-first protruding part, 34-second protruding part, 4-mounting platform, 41-flange, 42-guide sleeve, 43-supporting rod, 44-connecting plate, 5-ring cavity, 6-motor, 61-screw rod, 62-nut, 7-mounting plate, 71-guide rod, 8-sample tube. DETAILED DESCRIPTION

[0022] The utility model will be described in further detail below in combination with the drawings. The components or structures not limited in the utility model adopt the conventional technical means in the art.

[0023] The non-contact dry ultrasonic pulverizer of the embodiment, as shown in Figures 1-7 , includes a sample disc 1, a pressing seat 2, and an ultrasonic transducer 3. The sample disc 1 is provided with a plurality of sample holes 11 for placing sample tubes 8. The sample disc 1 is placed on the pressing seat 2. A flange 41 is mounted on a mounting platform 4. The ultrasonic transducer 3 is fixed to the flange 41. An ultrasonic tool head 31 is fixed to the upper end of the ultrasonic transducer 3. The pressing seat 2 is circumferentially arranged on the radial outer side of the ultrasonic tool head 31. The radial outer surface of the ultrasonic tool head 31 and the radial inner surface of the pressing seat 2 form a ring cavity 5. The ring cavity 5 is used to contact and cooperate with the tube wall of the sample tube 8 to realize the positioning of the sample tube 8.

[0024] In the embodiment, the sample tube 8 is obliquely inserted into the ring cavity 5, the ultrasonic tool head 31 is disc-shaped, the outer peripheral wall of the ultrasonic tool head 31 is provided with a plurality of first positioning grooves 32 which are upwardly and downwardly communicated and have circular arc cross sections, the inner peripheral wall of the pressing seat 2 is provided with a plurality of second positioning grooves 21 which are upwardly and downwardly communicated and have circular arc cross sections, the center lines of each first positioning groove 32 and each second positioning groove 21 are respectively inclined from top to bottom to the radial inner side of the ultrasonic tool head 31, the diameters of each first positioning groove 32 and each second positioning groove 21 are respectively matched with the outer diameter of the sample tube 8, the ring cavity 5 is surrounded by the plurality of first positioning grooves 32 and the plurality of second positioning grooves 21, the lower side of the mounting platform 4 is provided with a motor 6, the motor 6 is used to drive the mounting platform 4 to move up and down, so that the ultrasonic tool head 31 presses the tube wall of the sample tube 8. The motor 6 is mounted on a mounting plate 7, the top of the mounting plate 7 is vertically fixed with a plurality of guide rods 71, the upper ends of the plurality of guide rods 71 are respectively fixed to the pressing seat 2, a plurality of guide sleeves 42 are fixed on the mounting platform 4, each guide sleeve 42 is slidably sleeved on one guide rod 71, the bottom of the mounting platform 4 is vertically fixed with a plurality of supporting rods 43, the lower ends of the plurality of supporting rods 43 are respectively fixed on a connecting plate 44, the connecting plate 44 is fixed with a nut 62, the output end of the motor 6 is connected with a lead screw 61, the lead screw 61 is threadedly connected with the nut 62. When the motor 6 works, the power of the motor 6 is transmitted to the connecting plate 44 through the lead screw 61 and the nut 62, the connecting plate 44, the supporting rods 43, the mounting platform 4 and the ultrasonic transducer 3 are driven to stably descend or ascend along the guide rods 71 as a whole, so that the ultrasonic tool head 31 clamps or loosens the sample tube 8.

[0025] In the embodiment, the plurality of first positioning grooves 32 and the plurality of second positioning grooves 21 respectively have two diameters, so that two kinds of sample tubes 8 with different outer diameters can be placed on the sample disc 1 at the same time.

[0026] In the embodiment, the middle part of the ultrasonic tool head 31 is integrally provided with a first protruding part 33 which protrudes upward and a second protruding part 34 which protrudes downward, which can make the vibration and energy transmission of the ultrasonic tool head 31 more uniform.

[0027] The non-contact dry ultrasonic grinder is used as follows: firstly, the motor 6 works to drive the connecting plate 44, the supporting rod 43, the mounting platform 4 and the ultrasonic transducer 3 to stably ascend along the guide rod 71 by a certain distance; then, the operator inserts the sample tubes 8 containing samples into the sample holes 11 on the sample disc 1 respectively and inserts the sample tubes 8 into the ring cavity 5 so that the tube wall of the sample tube 8 is in contact with the ring cavity 5; after that, the motor 6 works to drive the connecting plate 44, the supporting rod 43, the mounting platform 4 and the ultrasonic transducer 3 to stably descend along the guide rod 71 by a certain distance, and the ultrasonic tool head 31 simultaneously presses the tube wall of the plurality of sample tubes 8 to ensure the ultrasonic grinding effect of the samples; finally, the ultrasonic transducer 3 is started to make the ultrasonic tool head 31 vibrate and transmit ultrasonic energy to the sample tubes 8 to dryly grind the samples. During the dry ultrasonic grinding process, the samples are not polluted, the sample tube 8 obliquely inserted into the ring cavity 5 has a large contact area with the ring cavity 5, the conversion efficiency of the ultrasonic energy is high, and the non-contact dry ultrasonic grinder is especially suitable for synchronous ultrasonic processing of a plurality of nucleic acid samples and can generally complete the efficient processing of the plurality of nucleic acid samples within 1 minute, which helps to greatly improve the nucleic acid extraction efficiency.

Claims

1. A non-contact dry-type ultrasonic disintegrator characterized by comprising: The utility model discloses a sample disc, compression seat and ultrasonic transducer, the sample disc is equipped with a plurality of sample holes for placing sample tubes, the sample disc is placed on the compression seat, the ultrasonic transducer is fixed on a mounting platform, the ultrasonic transducer is connected with ultrasonic tool head, the compression seat is arranged around the radial outer side of ultrasonic tool head, the radial outer surface of ultrasonic tool head and the radial inner surface of compression seat form annular cavity, the annular cavity is used for contact with the tube wall of sample tube to realize the positioning of sample tube, and the sample tube is inserted in the annular cavity.

2. A non-contact dry ultrasonic disintegrator according to claim 1, characterized in that, The ultrasonic tool head is discoid, the outer peripheral wall of ultrasonic tool head is equipped with a plurality of first positioning grooves of upper and lower communication of circular arc cross section, the inner peripheral wall of compression seat is equipped with a plurality of second positioning grooves of upper and lower communication of circular arc cross section, the center line of each first positioning groove and each second positioning groove is inclined to the radial inner side of ultrasonic tool head from top to bottom respectively, the diameter of each first positioning groove and each second positioning groove is adapted to the outer diameter of sample tube respectively, the annular cavity is formed by a plurality of first positioning grooves and a plurality of second positioning grooves, the lower side of mounting platform is provided with motor, and the motor is used to drive the up and down movement of mounting platform, so that ultrasonic tool head compresses the tube wall of sample tube.

3. A non-contact dry ultrasonic disintegrator according to claim 2, wherein The motor is installed on a mounting plate, the top of mounting plate is vertically fixed with a plurality of guide rods, the upper end of a plurality of guide rods is fixed to compression seat respectively, a plurality of guide sleeves are fixed on mounting platform, each guide sleeve is slidably sleeved on a guide rod, the bottom of mounting platform is vertically fixed with a plurality of supporting rods, the lower end of a plurality of supporting rods is fixed on a connecting plate respectively, the connecting plate is fixed with nut, the output end of motor is connected with lead screw, and the lead screw is threadedly connected with nut.

4. A non-contact dry ultrasonic disintegrator according to claim 2, wherein The diameters of a plurality of first positioning grooves are not completely same, and the diameters of a plurality of second positioning grooves are not completely same.

5. A non-contact dry ultrasonic disintegrator according to claim 1, wherein The mounting platform is installed with flange, the ultrasonic transducer is fixed to the flange, and the ultrasonic tool head is fixed on the upper end of ultrasonic transducer.

6. A non-contact dry ultrasonic disintegrator according to claim 1, wherein The middle part of ultrasonic tool head is integrally provided with first convex part and second convex part.

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