Electrophoresis device for monoclonal antibody quality inspection

Abstract

The electrophoresis device comprises an electrophoresis tank main body, cover plates are arranged at the two ends of the electrophoresis tank main body through lifting assemblies, the cover plates are connected with the electrophoresis tank main body through sealing assemblies, clamping assemblies are arranged on the cover plates through adjusting mechanisms, and the clamping assemblies are connected with the electrophoresis tank main body through adjusting mechanisms. And a placing assembly is arranged between the two clamping assemblies on the same side. According to the electrophoresis tank disclosed by the utility model, the arranged lifting assembly is matched with the adjusting mechanism, so that the cover plate can be conveniently driven to move up and down, the electrophoresis tank main body can be conveniently covered without influencing electrophoresis use, meanwhile, an electrophoresis plate and the like can be conveniently put in and moved out, and the electrophoresis plate and the like can be conveniently clamped and taken down by matching with the adjusting mechanism; and therefore, regular cleaning or replacement can be facilitated, and follow-up use is not affected.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological medicine preparation technical field, concretely is a kind of electrophoresis device for monoclonal antibody quality inspection. BACKGROUND

[0002] Monoclonal antibody is produced by artificial preparation hybridoma cell, and the hybridoma cell is formed by the fusion of a B cell activated by antigen and a myeloma cell, and the monoclonal antibody has the advantages of high purity, high sensitivity, strong specificity, less cross-reaction and low preparation cost, so that the monoclonal antibody can be conveniently detected, and in general, a special electrophoresis device is needed before detection, the monoclonal antibody solution is prepared, and then the monoclonal antibody solution is injected into a specially designed electrophoresis tank, and the electrodes in the electrophoresis tank are powered on, different charged particles are separated from each other due to different moving distances after a certain time of electrophoresis in the same electric field because of different charges or different charge-to-mass ratios, so that the monoclonal antibody can be more intuitively detected and analyzed.

[0003] However, the existing electrophoresis device for monoclonal antibody quality inspection has some defects in actual use, the electrophoresis plate is mostly fixedly installed in the electrophoresis tank, and is prone to corrosion or damage after long-term use, so that the electrophoresis plate cannot be conveniently cleaned or replaced, and residual proteins or other substances can affect subsequent electrophoresis quality inspection. UTILITY MODEL CONTENT

[0004] The utility model aims at providing an electrophoresis device for monoclonal antibody quality inspection to solve the problems in the background art.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: an electrophoresis device for monoclonal antibody quality inspection, comprising an electrophoresis tank main body, two ends of the electrophoresis tank main body are provided with cover plates through a lifting assembly, the cover plates and the electrophoresis tank main body are connected through a sealing assembly, the cover plates are provided with clamping assemblies through adjusting mechanisms, and a placing assembly is arranged between the two clamping assemblies on the same side.

[0006] Preferably, in order to conveniently move up and down with the cover plates and the like and facilitate operation, the lifting assembly comprises mounting plates symmetrically arranged at the two ends of the electrophoresis tank main body, and electric push rods one are symmetrically arranged on the mounting plates, and a connecting plate is arranged at the output end of the electric push rod one, and the connecting plate is fixedly connected with the cover plates.

[0007] Preferably, in order to ensure that the electrophoresis tank main body is sealed during use, the sealing assembly comprises a sealing groove opened at the opening end of the electrophoresis tank main body, and a sealing ring in interference fit with the sealing groove is arranged below the cover plate.

[0008] Preferably, in order to conveniently bring the clamping assembly close to each other and away, conveniently realize clamping and away from the removal operation, the adjusting mechanism comprises equidistantly opened grooves on the cover plate, the top end of the groove is provided with sealing cloth, the moving block is arranged in the groove through the electric push rod two, and the bottom end of the moving block is connected with the clamping assembly.

[0009] Preferably, in order to ensure stability during movement, the adjusting mechanism further comprises guide blocks symmetrically arranged on the two side walls of the moving block, and the inner wall of the groove is provided with a guide groove matched with the guide block.

[0010] Preferably, in order to conveniently clamp and position the placing assembly, without affecting electrophoresis quality inspection, the clamping assembly comprises a vertical plate fixedly arranged at the bottom end of the moving block, a clamping groove is formed in the vertical plate, and a clamping pad is arranged in the clamping groove.

[0011] Preferably, in order to conveniently perform electrophoresis quality inspection, the placing assembly comprises two electrophoresis plates arranged between the two clamping grooves on the same side, equidistant through holes are formed in the electrophoresis plates, and sponge layers are arranged on the side walls of the two electrophoresis plates close to each other, a colloid layer is arranged on one of the sponge layers, and a membrane layer is arranged on the other sponge layer.

[0012] Compared with the prior art, the utility model has the advantages that:

[0013] (1) through the lifting assembly and the adjusting mechanism, the cover plate can be conveniently moved up and down, the electrophoresis tank body can be conveniently covered, the use of electrophoresis is not affected, the electrophoresis plate can be conveniently put in and taken out, the adjusting mechanism can conveniently clamp and remove the electrophoresis plate, and the electrophoresis plate can be conveniently cleaned or replaced regularly without affecting the subsequent use;

[0014] (2) the sealing effect during use can be ensured by the sealing assembly, external pollution can be avoided, and the use of electrophoresis quality inspection is not affected; the clamping assembly and the adjusting mechanism can conveniently clamp and position the electrophoresis plate, and the electrophoresis plate can be conveniently removed, which facilitates detection, cleaning or replacement. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 A structure diagram of the electrophoresis device for monoclonal antibody quality inspection is provided.

[0016] Figure 2 A structure diagram of the lifting assembly and the cover plate in the electrophoresis device for monoclonal antibody quality inspection is provided.

[0017] Figure 3 A structure diagram of the cover plate in the electrophoresis device for monoclonal antibody quality inspection is provided.

[0018] Figure 4 Part structure schematic view of electrophoresis device for monoclonal antibody quality inspection is provided for the utility model;

[0019] Figure 5 Structure schematic view of placing assembly in electrophoresis device for monoclonal antibody quality inspection is provided for the utility model.

[0020] In the drawing: 1, electrophoresis tank main body;2, cover plate;3, mounting plate;4, electric push rod one;5, connecting plate;6, electrophoresis plate;7, sealing groove;8, sealing cloth;9, sealing ring;10, empty groove;11, vertical plate;12, clamping pad;13, through hole;14, electric push rod two;15, moving block;16, guide block;17, guide groove;18, sponge layer;19, colloid layer;20, membrane layer. Specific implementation

[0021] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0022] Please refer to Figures 1-5 The utility model provides an embodiment: a kind of electrophoresis device for monoclonal antibody quality inspection, including the electrophoresis tank main body 1 for the quality inspection of monoclonal antibody, it is mainly composed of electrophoresis tank, positive and negative electrode and buffer solution etc., to improve electrophoresis effect, avoid the influence of temperature on electrophoresis result, temperature control system (can be made of cooling and heating system) is also equipped in electrophoresis tank main body 1, more mature technology, here is not repeated, and the cover plate 2 of two ends of electrophoresis tank main body 1 is equipped with by lifting assembly, can be sealed to electrophoresis tank main body 1, prevent pollution etc., and the cover plate 2 is equipped with engaging assembly by adjusting mechanism, can be moved with the placing assembly between engaging assembly, can be conveniently inserted and removed operation, and for placing assembly (can be two groups, also can be three or four groups etc., can simultaneously to multiple groups electrophoresis quality inspection operation), it is composed of two electrophoresis plates 6, equidistant through hole 13 is formed on electrophoresis plate 6, and sponge layer 18 is equipped on the side wall of the mutual approach of electrophoresis plate 6, and on the side wall of the mutual approach of two sponge layers 18, one is equipped with colloid layer 19, and another is equipped with membrane layer 20, colloid layer 19 is attached with the protein to be handled, and protein can be migrated to membrane layer 20 by electrophoresis to realize quality inspection function, more mature technology, here is not repeated.

[0023] Please refer to Figures 1-5In order to facilitate the electrophoresis plate 6 up and down, the electrophoresis quality inspection operation can be conveniently carried out, and after completion, it is removed, and the cleaning or replacement operation can be conveniently taken out, the two ends of the electrophoresis tank body 1 are symmetrically welded with the mounting plate 3 of the lifting assembly, and the electric push rod one 4 is symmetrically vertically installed on the mounting plate 3. The output end (i.e. the top end) of the electric push rod one 4 is provided with a connecting plate 5, both connecting plates 5 are welded and fixed with the cover plate 2, and the bottom end of the cover plate 2 is bonded with the sealing ring 9 of the sealing assembly. Corresponding to the top end of the electrophoresis tank body 1, a sealing groove 7 is provided, which can be easily sealed during electrophoresis. In order to facilitate the clamping of the placing assembly, the electrophoresis quality inspection operation is facilitated, and the subsequent removal is facilitated, the cover plate 2 is equally provided with the air slot 10 of the adjusting mechanism, the top end of the air slot 10 is bonded with the sealing cloth 8 (the material can be rubber, or other materials, as long as the sealing effect is ensured), and the inside of the air slot 10 is provided with a moving block 15 through the electric push rod two 14. The moving block 15 can reciprocatingly slide in the air slot 10. In order to ensure that the sliding does not deviate, the two side walls of the moving block 15 are symmetrically welded with the guide block 16, and the inner wall of the air slot 10 is provided with the guide groove 17 for facilitating the sliding of the guide block 16. The bottom end of the moving block 15 is welded with the vertical plate 11 of the clamping assembly, the vertical plate 11 is provided with a clamping groove, and the clamping pad 12 is bonded in the clamping groove. The clamping pad 12 can be conveniently clamped to the placing assembly, thereby facilitating the electrophoresis quality inspection operation.

[0024] Working principle: when the utility model is used, two electrophoresis plates 6 are spliced, at this time the colloid layer 19 is attached with the protein to be treated, then it is placed between the two vertical plates 11, the electric push rod two 14 is retracted, the moving block 15 slides in the air slot 10, the guide block 16 slides in the guide groove 17, the two vertical plates 11 are close to each other, and the clamping pad 12 can clamp and position the electrophoresis plate 6, then the electric push rod one 4 is retracted, the cover plate 2 is moved downward as a whole, the electrophoresis plate 6 is returned to the inside of the electrophoresis tank body 1, at this time the sealing ring 9 is in the sealing groove 7, and the cover plate 2 can seal the electrophoresis tank body 1. The electrophoresis quality inspection operation can be carried out, after completion, the electric push rod one 4 is elongated, the cover plate 2 is moved upward as a whole, the electrophoresis plate 6 is moved out as a whole, then the electric push rod two 14 is elongated, the two moving blocks 15 slide away from each other in the air slot 10, the vertical plate 11 loses the clamping of the electrophoresis plate 6, and then the electrophoresis plate 6 can be conveniently taken out, the cleaning or replacement operation is facilitated, and the next operation is facilitated.

[0025] It is apparent for a person skilled in the art that the present application is not restricted to the details of the above exemplary embodiments, but that it can be implemented in other concrete forms without departing from the spirit or the essential characteristics of the present application. Therefore, the embodiments should be considered as exemplary only, and not limiting, the scope of the present application being defined by the appended claims rather than the above description, and all changes coming within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference signs in the claims should not be construed as limiting the claims concerned.

Claims

1. An electrophoresis device for quality control of monoclonal antibodies, comprising an electrophoresis tank body (1), characterized in that: Two ends of the electrophoresis tank body (1) are provided with cover plates (2) through lifting assemblies, the cover plates (2) and the electrophoresis tank body (1) are connected through sealing assemblies, the cover plates (2) are provided with clamping assemblies through adjusting mechanisms, and placing assemblies are arranged between the two clamping assemblies on the same side.

2. The electrophoresis device for quality inspection of monoclonal antibody according to claim 1, characterized in that: The lifting assembly comprises mounting plates (3) symmetrically arranged at the two ends of the electrophoresis tank body (1), the mounting plates (3) are symmetrically provided with electric push rods (4), the electric push rods (4) are provided with connecting plates (5) at output ends, and the connecting plates (5) are fixedly connected with the cover plates (2).

3. The electrophoresis device for quality inspection of monoclonal antibody according to claim 2, characterized in that: The sealing assembly comprises sealing grooves (7) formed in the opening ends of the electrophoresis tank body (1), and the cover plates (2) are provided with sealing rings (9) in interference fit with the sealing grooves (7).

4. The electrophoresis device for quality inspection of monoclonal antibody according to claim 3, characterized in that: The adjusting mechanism comprises empty grooves (10) equidistantly formed in the cover plates (2), the empty grooves (10) are provided with sealing cloths (8) at top ends, the empty grooves (10) are provided with moving blocks (15) through electric push rods (14), and the moving blocks (15) are connected with the clamping assemblies at bottom ends.

5. The electrophoresis device for quality inspection of monoclonal antibody according to claim 4, characterized in that: The adjusting mechanism further comprises guide blocks (16) symmetrically arranged on the two side walls of the moving blocks (15), and guide grooves (17) matched with the guide blocks (16) are formed in the inner walls of the empty grooves (10).

6. The electrophoresis device for quality inspection of monoclonal antibody according to claim 5, characterized in that: The clamping assembly comprises vertical plates (11) fixedly arranged at the bottom ends of the moving blocks (15), the vertical plates (11) are provided with clamping grooves, and the clamping grooves are provided with clamping pads (12) inside.

7. The electrophoresis device for quality inspection of monoclonal antibody according to claim 6, characterized in that: The placing assembly comprises two electrophoresis plates (6) arranged between the two clamping grooves on the same side, the electrophoresis plates (6) are equidistantly provided with through holes (13), and the side walls, which are close to each other, of the two electrophoresis plates (6) are provided with sponge layers (18), one of the sponge layers (18) is provided with a colloid layer (19), and the other sponge layer (18) is provided with a membrane layer (20).

Images