High-benefit probiotic protease rapid separation and purification device

Abstract

A high-efficiency probiotic protease rapid separation and purification device belongs to the technical field of protease separation and purification and comprises a base, two arc-shaped side plates are symmetrically and fixedly mounted on the upper surface of the base, and a centrifugal cylinder with a valve discharge pipe at the bottom is rotatably mounted between the two arc-shaped side plates. Supporting plates are fixedly mounted on the deviating surfaces of the two arc-shaped side plates, hydraulic telescopic rods are fixedly mounted on the upper surfaces of the supporting plates, a top plate is fixedly mounted between the upper surfaces of the two hydraulic telescopic rods, a flushing plate is fixedly mounted on the lower surface of the top plate, and a plurality of hanging rods are mounted below the flushing plate; a hollow-out plate is fixedly arranged among the lower surfaces of the plurality of hanging rods, a filter membrane is detachably arranged in the hollow-out plate, a pump body A is fixedly arranged on the upper surface of the top plate, the liquid inlet end of the pump body A is connected with a liquid pumping pipe, and the liquid pumping pipe penetrates through the flushing plate and is positioned above the hollow-out plate. And the process stability and reliability are enhanced.

Description

Technical Field

[0001] This invention belongs to the field of protease separation and purification technology, specifically relating to a high-efficiency protease rapid separation and purification device. Background Technology

[0002] In the field of biotechnology, the isolation and purification of probiotic proteases is an important task. With the deepening of research on probiotics and the expansion of their applications in industries such as medicine and food, the demand for efficient, rapid, and accurate protease isolation and purification devices is increasing.

[0003] A search revealed a patent in the relevant field with publication number CN215328032U, entitled "A Rapid Separation and Purification Device for Plant Proteases." While this device facilitates the removal of the supernatant from the centrifuge and repeated rinsing with water for purification, it lacks a suitable built-in filtration structure. Filtration of the supernatant requires transferring it to another filtration device, which not only increases the number of steps but also easily introduces new contaminants, affecting the purity of the protease. Therefore, this paper proposes a high-efficiency rapid separation and purification device for probiotic proteases to address these technical problems. Utility Model Content

[0004] In existing rapid separation and purification devices for plant proteases, the supernatant needs to be transferred to another filtration device during filtration, which poses a risk of supernatant contamination and affects protease purity. This invention addresses this issue with a high-efficiency rapid separation and purification device for probiotic proteases. After centrifugation of the probiotic protease solution in the centrifuge tube, water is injected through a rinsing plate to rinse the supernatant and raise its level. Impurities are blocked by the filter membrane, and the filtered supernatant, located above the perforated plate, can be easily extracted via pump A and the extraction tube. This design completes separation, purification, and filtration within the centrifuge tube, significantly reducing the risk of supernatant contamination and ensuring protease purity, unlike existing rapid separation and purification devices that require transferring the supernatant to other filtration devices. In terms of time efficiency, omitting the supernatant transfer step greatly shortens operation time and improves separation and purification efficiency. Furthermore, the optimized design allows rinsing, filtration, and extraction to occur simultaneously, reducing connection time and errors, and enhancing process stability and reliability. The specific technical solution is as follows:

[0005] A high-efficiency probiotic rapid separation and purification device includes a base. Two arc-shaped side plates are symmetrically fixedly installed on the upper surface of the base. A centrifuge cylinder with a discharge pipe with a valve at the bottom is rotatably installed between the two arc-shaped side plates. Support plates are fixedly installed on the opposite surfaces of the two arc-shaped side plates. Hydraulic telescopic rods are fixedly installed on the upper surfaces of the support plates. A top plate is fixedly installed between the upper surfaces of the two hydraulic telescopic rods. The top plate is located above the arc-shaped side plates, and a rinsing plate is fixedly installed on the lower surface of the top plate. Multiple hanging rods are installed below the rinsing plate. A perforated plate is fixedly installed between the lower surfaces of the multiple hanging rods. A filter membrane is detachably installed inside the perforated plate. A rubber ring is detachably installed on the outer surface of the perforated plate. The outer surface of the rubber ring is in contact with the inner surface of the centrifuge cylinder. A pump body A is fixedly installed on the upper surface of the top plate. A liquid extraction pipe is connected to the inlet end of the pump body A. The liquid extraction pipe passes through the rinsing plate and is located above the perforated plate. A liquid collection pipe is connected to the outlet end of the pump body A.

[0006] In the above technical solution, the lower surface of the rinsing plate is provided with multiple rinsing holes, and the water inlet end of the rinsing plate is connected to a water inlet pipe. The upper surface of the top plate is fixedly installed with a pump body B. The drain end of the pump body B is connected to the water inlet pipe. The water inlet end of the pump body B is connected to a water source pipe, and the water source pipe is connected to an external water tank.

[0007] In the above technical solution, the lower surface of the perforated plate is provided with an installation groove, and the outer surface of the perforated plate is screwed with a support ring, which clamps and fixes the filter membrane in the installation groove.

[0008] In the above technical solution, multiple steel balls are equidistantly embedded and rotatably mounted on the outer surface of the rinsing plate, and the outer surface of the steel balls is in contact with the inner surface of the centrifuge cylinder.

[0009] In the above technical solution, the outer surface of the hollow plate is integrally formed with protrusions, and the rubber ring is sleeved on the outer surface of the protrusions.

[0010] In the above technical solution, a motor is fixedly installed on the upper surface of the base, and the output end of the motor is fixedly connected to the center of the lower surface of the centrifuge tube.

[0011] In the above technical solution, an observation window is provided through the front surface of the centrifuge tube, and a transparent glass is sealed inside the observation window.

[0012] This utility model provides a high-efficiency rapid separation and purification device for probiotic proteases, which, compared with existing technologies, offers the following advantages:

[0013] The entire operation process of this invention, including separation, purification, and filtration, is completed within a centrifuge tube. Unlike existing rapid separation and purification devices for plant proteases that require transferring the supernatant to other filtration devices, this invention greatly reduces the risk of supernatant transfer contamination and ensures protease purity. In terms of time efficiency, the supernatant transfer step is omitted, significantly shortening the operation time and improving separation and purification efficiency. Furthermore, through optimized design, rinsing, filtration, and extraction are performed simultaneously, reducing the time and error between steps and enhancing process stability and reliability. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the main structure of this utility model.

[0015] Figure 2 This is a three-dimensional structural diagram of the present invention.

[0016] Figure 3 This is a cross-sectional structural diagram of the present invention.

[0017] Figure 4 This is a schematic cross-sectional view of the flushing plate of this utility model.

[0018] Figure 5 This is a schematic diagram of the lifting rod mechanism of this utility model.

[0019] Figure 1-Figure 5 The components are as follows: 1. Base; 11. Arc-shaped side plate; 2. Centrifuge cylinder; 21. Motor; 22. Discharge pipe with valve; 23. Observation window; 3. Support plate; 4. Hydraulic telescopic rod; 41. Top plate; 5. Washing plate; 51. Water inlet pipe; 52. Hanging rod; 53. Hollow plate; 531. Raised rib; 54. Filter membrane; 55. Support ring; 56. Rubber ring; 57. Steel ball bearing; 6. Pump body A; 61. Liquid extraction pipe; 62. Liquid collection pipe; 7. Pump body B; 71. Water source pipe. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] In this embodiment, front, back, left, right, top, and bottom are... Figure 1 Describe the reference plane. See [link / reference] Figure 1-Figure 5 This utility model provides a technical solution:

[0022] A high-efficiency probiotic protease rapid separation and purification device includes a base 1. Two arc-shaped side plates 11 are symmetrically fixedly installed on the upper surface of the base 1. A centrifuge cylinder 2 with a valved discharge pipe 22 at the bottom is rotatably installed between the two arc-shaped side plates 11. Support plates 3 are fixedly installed on the opposite surfaces of the two arc-shaped side plates 11. Hydraulic telescopic rods 4 are fixedly installed on the upper surfaces of the support plates 3. A top plate 41 is fixedly installed between the upper surfaces of the two hydraulic telescopic rods 4. The top plate 41 is located above the arc-shaped side plates 11, and the lower surface of the top plate 41 is fixed. A rinsing plate 5 is installed, and multiple hanging rods 52 are installed below the rinsing plate 5. A perforated plate 53 is fixedly installed between the lower surfaces of the multiple hanging rods 52. A filter membrane 54 is detachably installed inside the perforated plate 53. A rubber ring 56 is detachably installed on the outer surface of the perforated plate 53. The outer surface of the rubber ring 56 is in contact with the inner surface of the centrifuge cylinder 2. The setting of the rubber ring 56 ensures a tight fit between the perforated plate 53 and the centrifuge cylinder 2, ensuring that when the supernatant moves upward, it can pass through the filter membrane 54 to reach the top of the perforated plate 53, thus ensuring the filtration effect of the supernatant.

[0023] A pump body A6 is fixedly installed on the upper surface of the top plate 41. The inlet end of the pump body A6 is connected to a suction pipe 61. The suction pipe 61 passes through the flushing plate 5 and is located above the hollow plate 53. The outlet end of the pump body A6 is connected to a liquid collection pipe 62.

[0024] In use, the hydraulic telescopic rod 4 extends, causing the top plate 41 and its components below it—the rinsing plate 5, the hanging rod 52, the perforated plate 53, and the filter membrane 54—to move out of the centrifuge cylinder 2. The probiotic protease liquid to be separated and purified, along with the reaction agent, is then placed into the centrifuge cylinder 2. Afterward, the hydraulic telescopic rod 4 retracts to reset the top plate 41. The rotation of the centrifuge cylinder 2 then accelerates the reaction of the probiotic protease liquid, placing it in the supernatant. Simultaneously, the rinsing plate 5 continuously injects clean water into the centrifuge cylinder 2, which first contacts the supernatant to further react with the probiotic protease. During the cleaning process, the supernatant level rises. As it rises, the supernatant is filtered through the filter membrane 54. After that, stop injecting water into the centrifuge cylinder 2, start the pump body A6, and use the liquid extraction pipe 61 to extract the supernatant from the centrifuge cylinder 2. The remaining liquid in the centrifuge cylinder 2 needs to be discharged by opening the discharge pipe 22 with a valve. During use, since water is injected into the centrifuge cylinder 2 using the flushing plate 5, the filter membrane 54 can be backwashed using the flushing plate 5 when injecting water or when discharging the remaining waste in the centrifuge cylinder 2, thereby solving the problem of cleaning when it is clogged.

[0025] It should be noted that the lower surface of the rinsing plate 5 is provided with multiple rinsing holes, and the water inlet end of the rinsing plate 5 is connected to the water inlet pipe 51. The upper surface of the top plate 41 is fixedly installed with a pump body B7. The drain end of the pump body B7 is connected to the water inlet pipe 51. The water inlet end of the pump body B7 is connected to a water source pipe 71. The water source pipe 71 is connected to an external water tank. The pump body B7 uses the water source pipe 71 to supply clean water from the external water tank into the rinsing plate 5 through the water inlet pipe 51. Water is sprayed out from the rinsing holes to inject water or to rinse the filter membrane 54.

[0026] To facilitate the replacement of filter membrane 54, such as Figure 4 As shown, the lower surface of the perforated plate 53 has an installation groove, and the outer surface of the perforated plate 53 is screwed with a support ring 55. The support ring 55 clamps and fixes the filter membrane 54 in the installation groove. When replacing the filter membrane 54, first move the perforated plate 53 out of the centrifuge cylinder 2 as a whole through the hydraulic telescopic rod 4. Then, rotate the support ring 55 to separate it from the perforated plate 53, and the filter membrane 54 can be removed for replacement. When installing the filter membrane 54, the operation is reversed.

[0027] To enhance the stability of centrifuge tube 2 during rotation, such as Figure 4 As shown, multiple steel ball bearings 57 are equidistantly embedded and rotatably installed on the outer surface of the rinsing plate 5. The outer surface of the steel ball bearings 57 is in contact with the inner surface of the centrifuge cylinder 2. With the help of the position of the rinsing plate 5, the centrifuge cylinder 2 can be limited without affecting its rotation, thus preventing it from shaking during rotation and enhancing its stability.

[0028] Specifically, such as Figure 4 As shown, the outer surface of the perforated plate 53 is integrally formed with a raised rib 531, and the rubber ring 56 is sleeved on the outer surface of the raised rib 531. The rubber ring 56 is sleeved on the outer surface of the perforated plate 53 through the raised rib 531.

[0029] Specifically, a motor 21 is fixedly installed on the upper surface of the base 1, and the output end of the motor 21 is fixedly connected to the center of the lower surface of the centrifuge tube 2. The centrifuge tube 2 rotates by being driven by the motor 21.

[0030] Finally, to facilitate observation of the liquid inside centrifuge tube 2 and to facilitate timely extraction of the supernatant, such as... Figure 1 or Figure 2 As shown, an observation window 23 is provided through the front surface of the centrifuge tube 2. A transparent glass is sealed inside the observation window 23, allowing real-time observation of the liquid level inside the centrifuge tube 2, which facilitates timely extraction of the supernatant.

Claims

1. A high-efficiency probiotic protease rapid separation and purification device, comprising a base (1), characterized in that, Two arc-shaped side plates (11) are symmetrically fixedly installed on the upper surface of the base (1). A centrifuge cylinder (2) with a valved discharge pipe (22) at the bottom is rotatably installed between the two arc-shaped side plates (11). Support plates (3) are fixedly installed on the opposite surfaces of the two arc-shaped side plates (11). Hydraulic telescopic rods (4) are fixedly installed on the upper surface of the support plates (3). A top plate (41) is fixedly installed between the upper surfaces of the two hydraulic telescopic rods (4). The top plate (41) is located at the... A rinsing plate (5) is fixedly installed above the arc-shaped side plate (11) and on the lower surface of the top plate (41). Multiple hanging rods (52) are installed below the rinsing plate (5). A perforated plate (53) is fixedly installed between the lower surfaces of the multiple hanging rods (52). A filter membrane (54) is detachably installed inside the perforated plate (53). A rubber ring (56) is detachably installed on the outer surface of the perforated plate (53). The outer surface of the rubber ring (56) is in contact with the inner surface of the centrifuge cylinder (2). A pump body A (6) is fixedly installed on the upper surface of the top plate (41). The inlet end of the pump body A (6) is connected to a suction pipe (61). The suction pipe (61) passes through the flushing plate (5) and is located above the hollow plate (53). The outlet end of the pump body A (6) is connected to a liquid collection pipe (62).

2. The high-efficiency probiotic protease rapid separation and purification device according to claim 1, characterized in that, The lower surface of the flushing plate (5) is provided with a plurality of flushing holes, and the water inlet end of the flushing plate (5) is connected to a water inlet pipe (51). A pump body B (7) is fixedly installed on the upper surface of the top plate (41). The drain end of the pump body B (7) is connected to the inlet pipe (51). The inlet end of the pump body B (7) is connected to a water source pipe (71). The water source pipe (71) is connected to an external water tank.

3. The high-efficiency probiotic protease rapid separation and purification device according to claim 1, characterized in that, The lower surface of the perforated plate (53) is provided with an installation groove, and a support ring (55) is screwed onto the outer surface of the perforated plate (53). The support ring (55) clamps and fixes the filter membrane (54) in the installation groove.

4. The high-efficiency probiotic protease rapid separation and purification device according to claim 1, characterized in that, Multiple steel ball bearings (57) are equidistantly embedded and rotatably mounted on the outer surface of the rinsing plate (5), and the outer surface of the steel ball bearings (57) is in contact with the inner surface of the centrifuge cylinder (2).

5. The high-efficiency probiotic protease rapid separation and purification device according to claim 1, characterized in that, The outer surface of the perforated plate (53) is integrally formed with a raised rib (531), and the rubber ring (56) is fitted on the outer surface of the raised rib (531).

6. The high-efficiency probiotic protease rapid separation and purification device according to claim 1, characterized in that, A motor (21) is fixedly installed on the upper surface of the base (1), and the output end of the motor (21) is fixedly connected to the center of the lower surface of the centrifuge tube (2).

7. The high-efficiency probiotic protease rapid separation and purification device according to claim 1, characterized in that, An observation window (23) is provided through the front surface of the centrifuge tube (2), and a transparent glass is sealed inside the observation window (23).

Images