Multi-cell culture dish for co-culture of cells
By designing a multi-compartment cell co-culture dish and utilizing the Transwell filter membrane and clamping groove structure, the problem that traditional Transwell chamber cups are difficult to simulate complex cell growth environments is solved, and the co-culture of multiple cells and convenient operation are achieved.
Patent Information
- Application Number
- CN202422855673.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-22
- Publication Date
- 2025-10-28
- Estimated Expiration
- 2034-11-22
AI Technical Summary
Traditional Transwell chamber cups can only achieve co-culture of two types of cells in the cup and the well, and it is difficult to simulate a more complex cell growth environment.
A multi-compartment cell co-culture culture dish is designed, which includes a pad and multiple culture compartments arranged at the bottom of the culture dish. A Transwell filter membrane and a clamping groove structure are used to achieve independent inoculation and co-culture of multiple cells.
It achieves co-culture of at least 6 different cell types, simulating a more complex cell growth environment. It is easy to operate and the culture grids can be moved by clamps.
Smart Images
Figure CN223481137U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to a culture dish, specifically a multi-compartment culture dish for cell co-culture. Background Technology
[0002] The Transwell assay is used to study cell migration and invasion. It utilizes a transwell chamber with a porous membrane placed in a culture dish, separating cells into upper and lower chambers. Cells can migrate from the upper chamber to the lower chamber through the porous membrane based on the chemical gradient between the upper and lower chambers or stimulation from the extracellular matrix. By counting the number of migrating or invading cells, cell behavior and function can be assessed.
[0003] However, the traditional transwell cups mentioned above, placed above the culture wells of a cell culture plate, can only achieve co-culture of two types of cells in the cup and well, making it difficult to simulate more complex cell growth environments. Utility Model Content
[0004] To simulate a more complex cell growth environment, this invention provides a multi-compartment culture dish for cell co-culture.
[0005] The objective of this utility model can be achieved through the following technical solutions:
[0006] A multi-compartment cell co-culture dish, comprising the culture dish and further comprising:
[0007] A plate is placed at the bottom of the petri dish, and the plate is divided into multiple containment areas at equal intervals with its center as the reference.
[0008] Multiple culture compartments are arranged within multiple containment areas. Each culture compartment includes: a base plate; a first arc-shaped plate and a second arc-shaped plate arranged parallel to each other on the base plate; and two straight plates connecting the first and second arc-shaped plates, with a Transwell filter membrane disposed in the middle of each straight plate. The Transwell filter membranes of adjacent culture compartments abut against each other.
[0009] Furthermore, a partition strip is provided between two adjacent receiving areas; the partition strip has an inwardly concave arc transition on both sides.
[0010] Furthermore, the outer diameter of the pad is equal to the inner diameter of the culture dish.
[0011] Furthermore, the first arc-shaped plate is provided with a first clamping groove above the Transwell filter membrane; the second arc-shaped plate is provided with a second clamping groove and a third clamping groove at intervals above the Transwell filter membrane.
[0012] Furthermore, the connection points between the base plate, the first arc-shaped plate, the second arc-shaped plate, and the two straight plates are provided with outwardly convex arc-shaped transitions.
[0013] Furthermore, the Transwell filter membrane is one of a polycarbonate filter membrane, a polytetrafluoroethylene filter membrane, or a polyethylene terephthalate filter membrane.
[0014] Furthermore, the micropore size of the Transwell filter membrane is 0.1μm-12.0μm.
[0015] Furthermore, the culture compartments have at least six sections.
[0016] Compared with the prior art, the present invention has the following advantages:
[0017] (1) This utility model provides a multi-compartment culture dish for cell co-culture. The culture dish allows different cells to be inoculated independently through multiple culture compartments. After the cells adhere to the wall, culture medium is added to the extent that it exceeds the lower end of the Transwell filter membrane but does not exceed the top end of the Transwell filter membrane, thereby achieving co-culture.
[0018] (2) This utility model provides a multi-compartment cell co-culture dish, which allows the operator to move the culture compartments by using a three-jaw clamp to grip the clamping slots set on the arc-shaped plate.
[0019] (3) This utility model provides a multi-compartment cell co-culture dish that can realize transwell co-culture of at least 6 different cell types, simulating a more complex cell growth environment. Attached Figure Description
[0020] Figure 1 This is a schematic diagram of a multi-compartment culture dish used for co-culturing cells in the example.
[0021] Figure 2 This is a three-dimensional assembly diagram of the multi-compartment cell co-culture dish in the embodiment;
[0022] Figure 3 This is a cross-sectional view of a multi-compartment cell co-culture dish used in the example;
[0023] Figure 4 for Figure 3 A magnified view of a portion of region A in the middle;
[0024] Figure 5 This is a schematic diagram of the cultivation compartments in the embodiment;
[0025] The numbers in the diagram indicate: 1-Cultural dish; 2-Plate; 21-Containing area; 22-Separator strip; 3-Cultural compartment; 31-Straight plate; 32-Transwell filter membrane; 33-First arc-shaped plate; 34-First clamping groove; 35-Bottom plate; 36-Second clamping groove; 37-Second arc-shaped plate; 38-Third clamping groove. Detailed Implementation
[0026] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments. These embodiments are implemented based on the technical solution of the present invention, providing detailed implementation methods and specific operating procedures. However, the scope of protection of the present invention is not limited to the following embodiments.
[0027] Example
[0028] To simulate a more complex cell growth environment, this embodiment provides a multi-compartment cell co-culture dish, the specific structure of which is shown in [reference needed]. Figure 1-5 The petri dish 1 includes a plate 2 disposed at the bottom of the petri dish 1, wherein the plate 2 is divided into multiple accommodating areas 21 at equal intervals with its center as a reference.
[0029] Multiple culture compartments 3 are arranged within multiple containment areas. Each culture compartment 3 includes: a base plate 35; a first arc-shaped plate 33 and a second arc-shaped plate 37 arranged parallel to each other on the base plate; and two straight plates 31 connecting the first arc-shaped plate 33 and the second arc-shaped plate 37, respectively. A Transwell filter membrane 32 is disposed in the middle of each straight plate 31. The Transwell filter membranes 32 of two adjacent culture compartments 3 abut against each other.
[0030] In this embodiment, the outer diameter of the pad 2 is equal to the inner diameter of the culture dish 1; optionally, the culture compartments 3 have 6, 9 or even more compartments.
[0031] Please see again. Figure 4 In this embodiment, a partition strip 22 is provided between two adjacent receiving areas 21; the partition strip 22 has an inwardly concave arc transition on both sides.
[0032] Please see again. Figure 5 In this embodiment, the first arc-shaped plate 33 is provided with a first clamping groove 34 above the Transwell filter membrane 32; the second arc-shaped plate 37 is provided with a second clamping groove 36 and a third clamping groove 38 at intervals above the Transwell filter membrane 32.
[0033] Please see again. Figure 5 In this embodiment, the bottom plate 35, the first arc plate 33, the second arc plate 37 and the two straight plates 31 are provided with outwardly convex arc transitions at the connection points between each other.
[0034] In this embodiment, the Transwell filter membrane 32 is one of a polycarbonate filter membrane, a polytetrafluoroethylene filter membrane, or a polyethylene terephthalate filter membrane; the micropore size of the Transwell filter membrane 32 is 0.1 μm-12.0 μm.
[0035] In use, place the pad 2 into the culture dish 1, and then place the culture compartment 3 into the receiving area 21 of the pad 1 using the three-claw clamp. When inoculating cells, the culture medium added to the culture compartment 3 should not exceed the lower end of the Transwell filter membrane 32. After the cells in each compartment have adhered to the wall, add more culture medium so that the liquid level covers the lower end of the Transwell filter membrane 32 but does not exceed the top end of the Transwell filter membrane 32 to achieve co-culture. When it is necessary to remove any compartment, it can be removed using the three-claw clamp.
[0036] The above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model in any other way. Any person skilled in the art may make changes or modifications to the above-disclosed technical content to create equivalent embodiments. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of this utility model without departing from its technical solution shall still fall within the protection scope of this utility model.
Claims
1. A multi-compartment cell co-culture dish, comprising a culture dish (1), characterized in that, Also includes: A pad (2) is placed at the bottom of the petri dish (1), and the pad (2) is divided into multiple accommodating areas (21) at equal intervals with its center as the reference. Multiple culture compartments (3) are arranged within multiple containment areas, wherein the culture compartments (3) include: Base plate (35); The first arc-shaped plate (33) and the second arc-shaped plate (37) are arranged in parallel on the base plate; Two straight plates (31) are respectively connected to the first arc plate (33) and the second arc plate (37), and a Transwell filter membrane (32) is provided in the middle of the straight plate (31).
2. The multi-compartment culture dish for cell co-culture according to claim 1, characterized in that, A dividing strip (22) is provided between two adjacent receiving areas (21).
3. The multi-compartment culture dish for cell co-culture according to claim 2, characterized in that, The two sides of the separator (22) are provided with an inwardly concave arc transition.
4. The multi-compartment culture dish for cell co-culture according to claim 1, characterized in that, The outer diameter of the pad (2) is equal to the inner diameter of the culture dish (1).
5. The multi-compartment culture dish for cell co-culture according to claim 1, characterized in that, The first arc-shaped plate (33) is provided with a first clamping groove (34) above the Transwell filter membrane (32); the second arc-shaped plate (37) is provided with a second clamping groove (36) and a third clamping groove (38) at intervals above the Transwell filter membrane (32).
6. The multi-compartment culture dish for cell co-culture according to claim 1, characterized in that, The base plate (35), the first arc plate (33), the second arc plate (37) and the two straight plates (31) are connected by outwardly convex arc transitions.
7. The multi-compartment culture dish for cell co-culture according to claim 1, characterized in that, The Transwell membranes (32) of two adjacent culture compartments (3) abut against each other.
8. A multi-compartment culture dish for cell co-culture according to claim 1, characterized in that, The Transwell filter membrane (32) is one of a polycarbonate filter membrane, a polytetrafluoroethylene filter membrane, or a polyethylene terephthalate filter membrane.
9. A multi-compartment culture dish for cell co-culture according to claim 1, characterized in that, The micropore size of the Transwell filter membrane is 0.1μm-12.0μm.
10. A multi-compartment culture dish for cell co-culture according to claim 1, characterized in that, The culture compartments (3) have at least 6 compartments.