Immune cell culture tank

Abstract

The utility model relates to an immune cell culture tank, and relates to the field of biological cells, the immune cell culture tank comprises a bottle body with a cavity, a hydrophobic gas-permeable membrane is arranged in the bottle body, so that the cavity in the bottle body is divided into an upper culture cavity and a lower gas cavity, the gas cavity is communicated with an external mixed gas supply system through a gas inlet pipe, and the gas inlet pipe is communicated with the culture cavity. A gas guide pipe is arranged on the bottle body, one end of the gas guide pipe is communicated with the gas cavity, the other end of the gas guide pipe is communicated with the culture cavity and located above the culture solution in the culture cavity, and an exhaust pipe communicated with the culture cavity is arranged at the top of the bottle body. The device has the effect of improving the exchange efficiency between the mixed gas and the culture solution by simultaneously carrying out gas exchange on the cell culture solution through the upper side and the lower side.

Description

Technical Field

[0001] This application relates to the field of biological cells, and in particular to an immune cell culture vessel. Background Technology

[0002] Cell culture is a method of simulating the in vivo environment (sterile, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions.

[0003] Chinese patent CN219653037U discloses a suspended immune cell culture flask. An oxygen tube is connected to an oxygen source, and a carbon dioxide tube is connected to a carbon dioxide source. When a temperature sensor detects that the temperature inside the flask is too low, a control box heats the inside of the flask by controlling a heating mechanism. When an oxygen concentration sensor detects that the oxygen concentration inside the flask is too low, a control valve opens, allowing oxygen to be injected into the flask through the oxygen tube, gas chamber, and hydrophobic permeable membrane. When a carbon dioxide concentration sensor detects that the carbon dioxide concentration inside the flask is too low, a control valve opens, allowing carbon dioxide to be injected into the flask through the carbon dioxide tube, gas chamber, and hydrophobic permeable membrane. This invention can precisely control the temperature, carbon dioxide concentration, and oxygen concentration inside the culture flask, ensuring the activity of immune cells and achieving higher amplification efficiency. However, the oxygen and carbon dioxide mixture only exchanges with the culture medium through the hydrophobic permeable membrane at the bottom. The height of the culture medium is limited, and adding too much culture medium can lead to a concentration difference between the top and bottom of the culture medium, reducing the exchange efficiency between the mixed gas and the culture medium. Utility Model Content

[0004] To address the issue of reduced exchange efficiency between the mixed gas and the culture medium due to the gas-mixed mixture only exchanging with the culture medium through the hydrophobic and breathable membrane at the bottom, this application provides an immune cell culture vessel.

[0005] The immune cell culture vessel provided in this application adopts the following technical solution:

[0006] An immune cell culture vessel includes a bottle body with a cavity. A hydrophobic and breathable membrane is provided inside the bottle body to divide the cavity into an upper culture chamber and a lower gas chamber. The gas chamber is connected to an external mixed gas supply system via an air inlet pipe. A gas guide pipe is provided on the bottle body, with one end connected to the gas chamber and the other end connected to the culture chamber, and located above the culture medium in the culture chamber. An exhaust pipe connected to the culture chamber is provided at the top of the bottle body.

[0007] By adopting the above technical solution, when culturing cells, the mixed gas enters the gas chamber through the inlet pipe. The mixed gas is divided into two streams. One stream exchanges with the cell culture medium above through the hydrophobic and breathable membrane, while the other stream is guided to the top of the cell culture medium through the gas guide tube to exchange with the culture medium. By exchanging gas with the cell culture medium from both the top and bottom sides simultaneously, the exchange efficiency between the mixed gas and the culture medium is improved.

[0008] In one specific implementation, a mounting plate is fixedly provided inside the bottle, the mounting plate has multiple through holes, the hydrophobic and breathable membrane is laid on the mounting plate, and a fixing ring is provided inside the bottle to press the hydrophobic and breathable membrane against the mounting plate.

[0009] By adopting the above technical solution, the mixed gas and culture medium can be exchanged through the through holes on the mounting plate. The hydrophobic and breathable membrane is placed on the mounting plate and then fixed by the fixing ring, thereby improving the stability of the hydrophobic and breathable membrane installation.

[0010] In one specific implementation, both the intake pipe and the exhaust pipe are connected in series with sterilization filters.

[0011] In one specific implementation, a one-way valve is connected in series on the intake pipe.

[0012] By adopting the above technical solution, a one-way valve is used to prevent the backflow of mixed gas and ensure the normal flow of gas.

[0013] In one specific implementation, the bottle body is provided with a feeding tube that communicates with the culture chamber.

[0014] By adopting the above technical solution, nutrients in the cell culture medium can be replenished using a feeding tube, thereby improving the convenience of nutrient replenishment in the culture medium.

[0015] In one specific implementation, the bottle body is provided with a collection tube, the bottom of which extends to the hydrophobic and breathable membrane.

[0016] By adopting the above technical solution, cells on the hydrophobic and breathable membrane can be collected using a collection tube, thereby improving the convenience of cell collection.

[0017] In one specific implementation, the bottle body is provided with a sampling tube for insertion into the culture chamber, the bottom of the sampling tube being higher than the bottom of the collection tube.

[0018] By adopting the above technical solution, during the cell culture process, sampling tubes are used to sample the supernatant or cells in the culture medium, which facilitates the monitoring of cell growth status.

[0019] In one specific implementation, the bottle is provided with a pH sensor and a dissolved oxygen sensor that are inserted into the culture chamber.

[0020] By adopting the above technical solution, pH and dissolved oxygen sensors can be used to detect the acidity and alkalinity of the culture medium and the content of dissolved oxygen in a timely manner, thereby adjusting the type and amount of air intake and adjusting the changes in the microenvironment inside the tank.

[0021] In summary, this application includes at least one of the following beneficial technical effects:

[0022] 1. During cell culture, the mixed gas enters the gas chamber through the inlet tube. The mixed gas is divided into two streams. One stream exchanges gas with the cell culture medium above through the hydrophobic and gas-permeable membrane. The other stream is guided to the top of the cell culture medium through the gas guide tube to exchange gas with the culture medium. By exchanging gas with the cell culture medium from both the top and bottom, the exchange efficiency between the mixed gas and the culture medium is improved.

[0023] 2. The sterilization filter on the exhaust pipe can prevent the gas from escaping too quickly and block bacteria from entering, while the sterilization filter on the intake pipe can filter bacteria in the gas.

[0024] 3. Using pH and dissolved oxygen sensors, the acidity and alkalinity of the culture medium and the dissolved oxygen content can be detected in a timely manner, thereby adjusting the type and amount of air introduced and adjusting the changes in the microenvironment inside the tank. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the structure of an immune cell culture vessel according to an embodiment of this application.

[0026] Figure 2 It is along Figure 1 A cross-sectional view along line AA in the middle.

[0027] Figure 3 yes Figure 2 Enlarged view of section B in the middle.

[0028] Explanation of reference numerals in the attached diagram: 1. Bottle body; 11. Bottle cap; 12. Culture chamber; 13. Gas chamber; 2. Hydrophobic and breathable membrane; 3. Air inlet pipe; 31. One-way valve; 32. Sterilizing filter; 4. Air delivery pipe; 5. Exhaust pipe; 61. Feeding pipe; 62. Collection pipe; 63. Sampling pipe; 64. Mounting pipe; 65. pH sensor; 66. Dissolved oxygen sensor; 71. Mounting plate; 72. Fixing ring. Detailed Implementation

[0029] The following is combined with Figure 1-3 This application is described in further detail.

[0030] This application discloses an immune cell culture vessel.

[0031] Reference Figure 1 , Figure 2 An immune cell culture flask includes a bottle body 1, a hydrophobic and breathable membrane 2, an air inlet tube 3, an air delivery tube 4, and an exhaust tube 5. The bottle body 1 is cylindrical and can be made of materials such as plastic or glass. The design can be adjusted in size according to the actual needs of the laboratory. Plastic bottles can be made of materials with good chemical resistance and transparency to facilitate observation of changes in the culture medium. Glass bottles are made of materials with excellent thermal stability and are suitable for cell culture after high temperature and high pressure sterilization. The bottom of the bottle body 1 is sealed, and the top is detachably connected to a bottle cap 11. The hydrophobic and breathable membrane 2 is installed inside the bottle body 1, dividing the cavity inside the bottle body 1 into an upper culture chamber 12 and a lower gas chamber 13. The gas chamber 13 is connected to an external mixed gas supply system through an air inlet pipe 3. One end of the gas guide pipe 4 is connected to the gas chamber 13, and the other end is connected to the culture chamber 12, and is located above the culture medium in the culture chamber 12. The exhaust pipe 5 is installed on the top cap 11 of the bottle body 1 and is connected to the culture chamber 12. This achieves gas exchange between the cell culture medium and the mixed gas through both the upper and lower sides, improving the exchange efficiency of the mixed gas and the cell culture medium, and increasing the cell survival rate and working efficiency.

[0032] Reference Figure 2 The hydrophobic and breathable membrane 2 can be made of materials such as microporous membranes or PTFE membranes. The advantages of microporous membranes are their high air permeability and low cost, while the advantages of PTFE membranes are their good chemical stability and temperature resistance. Regardless of the material chosen, the membrane should have good air permeability while maintaining hydrophobic properties to prevent liquid from penetrating into the gas chamber 13.

[0033] Reference Figure 2 , Figure 3 An installation plate 71 is fixedly installed inside the bottle body 1. The installation plate 71 has multiple through holes. The hydrophobic and breathable membrane 2 is laid on the installation plate 71. A fixing ring 72 is provided inside the bottle body 1. The fixing ring 72 is made of rubber. The fixing ring 72 is pressed against the inner wall of the bottle body 1 by its own elastic deformation, and presses the hydrophobic and breathable membrane 2 against the installation plate 71, thereby completing the installation of the hydrophobic and breathable membrane 2.

[0034] Reference Figure 1 , Figure 2The intake pipe 3 can be a flexible PVC pipe or other materials suitable for gas transmission. To ensure a stable gas supply, a one-way valve 31 is connected in series on the intake pipe 3. The one-way valve 31 can be a duckbill valve or a spring-loaded one-way valve. The intake pipe 3 can control the intake volume through a flow controller and be connected to multiple gases, including but not limited to two, three, or four of them. The multiple gases refer to sterile compressed air, oxygen, carbon dioxide, and nitrogen. The mixed gas is introduced into the gas chamber 13. A sterilization filter 32 is connected in series on the intake pipe 3. The sterilization filter 32 is installed after the flow controller and connected to the one-way valve.

[0035] Reference Figure 1 , Figure 2 The gas delivery tube 4 can be a flexible tube or a fixed passage made of the same material as the bottle body. One end of the gas delivery tube 4 is located at the bottom of the bottle body 1 and communicates with the gas chamber 13, while the other end is located at the top of the bottle body 1, at the cap 11, and communicates with the culture chamber 12. This design can effectively increase the contact area between the mixed gas and the culture medium, thereby improving the gas exchange efficiency.

[0036] Reference Figure 1 , Figure 2 The exhaust pipe 5 is also made of soft material for easy connection and disassembly. A certain back pressure is set inside the exhaust pipe 5, so that the gas above the culture medium slowly flows out of the exhaust pipe 5, ensuring gas exchange between the cell culture medium and the gas chamber 13. A sterilization filter 32 is installed on the exhaust pipe 5.

[0037] Reference Figure 1 , Figure 2 The bottle cap 11 of the bottle body 1 is provided with a feeding tube 61. The main function of the feeding tube 61 is to replenish the required nutrients in a timely manner during cell culture. The number of feeding tubes 61 can be designed according to the needs. The feeding tube 61 can be designed as a quick connector.

[0038] Reference Figure 1 , Figure 2 The bottle cap 11 of the bottle body 1 is provided with a collection tube 62. The collection tube 62 is inserted into the culture chamber 12 from top to bottom and extends to the hydrophobic and breathable membrane 2. The collection tube 62 is mainly used to collect cells.

[0039] The bottle body 1 is equipped with a sampling tube 63, which is set on the bottle cap 11 and inserted into the culture chamber 12 from top to bottom. The bottom of the sampling tube 63 is higher than the bottom of the collection tube 62, which makes it easy to extract samples for analysis and testing, so as to keep track of the cell growth status at any time.

[0040] Reference Figure 1 , Figure 2 , refer to Figure 1 , Figure 2The bottle body 1 is provided with two mounting tubes 64, which are set on the lid or on the side of the bottle body 1 and are inclinedly connected to the bottle body 1. The two mounting tubes 64 are respectively provided with a pH sensor 65 and a dissolved oxygen sensor 66. Preferably, they are installed on the side so that the sensor probes are closer to the cell growth area, so as to accurately regulate the pH and dissolved oxygen in the tank.

[0041] In addition, thermometers, biochemical analysis probes and other measuring elements can be installed on bottle 1 to monitor the indicators of cell culture medium. A heating blanket can also be installed on bottle 1 to control the temperature of cell culture.

[0042] The implementation principle of an immune cell culture vessel according to an embodiment of this application is as follows: When culturing cells, the mixed gas enters the gas chamber 13 through the air inlet pipe 3. The mixed gas is divided into two streams. One stream exchanges with the cell culture medium above through the hydrophobic and breathable membrane 2, and the other stream is guided to the top of the cell culture medium through the air guide pipe 4 to exchange with the culture medium. By exchanging gas with the cell culture medium on both the upper and lower sides at the same time, the exchange efficiency between the mixed gas and the culture medium is improved.

[0043] The above are all preferred embodiments of the present application, and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the scope of protection of the present application.

Claims

1. An immune cell culture vessel, characterized in that: The device includes a bottle body (1) with a cavity. The bottle body (1) is provided with a hydrophobic and breathable membrane (2) so that the cavity inside the bottle body (1) is divided into an upper culture chamber (12) and a lower gas chamber (13). The gas chamber (13) is connected to an external mixed gas supply system through an air inlet pipe (3). The bottle body (1) is provided with a gas guide pipe (4). One end of the gas guide pipe (4) is connected to the gas chamber (13), and the other end is connected to the culture chamber (12) and is located above the culture liquid in the culture chamber (12). The top of the bottle body (1) is provided with an exhaust pipe (5) connected to the culture chamber (12).

2. The immune cell culture vessel according to claim 1, characterized in that: An installation plate (71) is fixedly provided inside the bottle body (1). The installation plate (71) has multiple through holes. The hydrophobic and breathable membrane (2) is laid on the installation plate (71). A fixing ring (72) is provided inside the bottle body (1). The fixing ring (72) can press the hydrophobic and breathable membrane (2) against the installation plate (71).

3. The immune cell culture vessel according to claim 1, characterized in that: Both the air intake pipe (3) and the exhaust pipe (5) are connected in series with a sterilization filter (32).

4. An immune cell culture flask according to claim 1, characterized in that: A one-way valve (31) is connected in series on the air intake pipe (3).

5. An immune cell culture flask according to claim 1, characterized in that: The bottle body (1) is provided with a feeding tube (61) that is connected to the culture chamber (12).

6. An immune cell culture flask according to claim 1, characterized in that: The bottle body (1) is provided with a collection tube (62), the bottom of which extends to the hydrophobic and breathable membrane (2).

7. An immune cell culture vessel according to claim 6, characterized in that: The bottle body (1) is provided with a sampling tube (63) for inserting into the culture chamber (12), and the bottom of the sampling tube (63) is higher than the bottom of the collection tube (62).

8. An immune cell culture flask according to claim 1, characterized in that: The bottle (1) is equipped with a pH sensor (65) and a dissolved oxygen sensor (66) that are inserted into the culture chamber (12).

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