Fermentation system for culturing L-homoserine and efficiently culturing homoserine seeds

Abstract

The utility model discloses a fermentation system for efficiently culturing homoserine seeds for culturing L-homoserine, and belongs to the technical field of microbial fermentation. The problems of low fermentation efficiency and high cost in the prior art are solved. The right side of the first pipeline is connected below the stirring tank; the left side of the second pipeline is connected with a first sewage tank; the right side of the third pipeline is connected with the left side of the stirring tank; the right side of the fourth pipeline is connected with the first pipeline; and the right side of the fifth pipeline is connected with the fourth pipeline. A seed stock solution is inoculated into the seed tank in a differential pressure inoculation mode, the temperature, the PH value, the ventilation quantity, the pressure and the dissolved oxygen concentration in the seed tank are controlled according to process requirements, it is guaranteed that strains grow rapidly in the environment most suitable for growth, production cost is reduced, and meanwhile the inoculation amount of a fermentation tank in the later period is met.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of microbial fermentation technology, specifically for a kind of high-efficiency cultivation of the fermentation system of high-spermidine seed of culture L-high spermidine. BACKGROUND

[0002] In biological fermentation, the seed tank plays a crucial role. It can heat, maintain temperature, and cool, providing special conditions for fully enclosed and sanitary fermentation. Its design aims to support the growth and reproduction of the bacterial strain by providing an environment that controls the fermentation conditions, promoting the rapid growth and reproduction of the bacterial strain, thereby forming a certain amount and quality of bacterial bodies. The role of the seed tank is not limited to spore germination and bacterial reproduction, but also includes optimizing the fermentation process to ensure the quality and yield of the final product.

[0003] In microbial fermentation production, the seed fermentation tank plays a crucial role. It is not only used for bacterial strain culture and fermentation, but also promotes bacterial proliferation, laying a solid foundation for subsequent fermentation processes. Through the cultivation of the seed tank, the bacterial bodies introduced into the fermentation tank can grow rapidly, reaching a certain amount of bacterial bodies, thereby facilitating product synthesis. This process is of great significance for improving fermentation efficiency and optimizing product quality.

[0004] In the prior art, there are some problems with the direct introduction of seeds into the fermentation tank, which may lead to a decrease in production capacity, premature autolysis of mycelium, changes in fermentation products, and an extension of the fermentation cycle.

[0005] After too old seeds are introduced into the fermentation tank, it may cause premature autolysis of mycelium, affecting the growth and reproduction of microorganisms. High temperature can cause premature autolysis of mycelium, which will affect the growth of the bacterial strain and the formation of fermentation products. At the same time, if the conditions are not suitable after the seed is introduced into the fermentation tank, it may cause changes in the chemical structure or properties of the fermentation product, thereby affecting the quality and use of the final product. After too young seeds are introduced into the fermentation tank, the fermentation cycle is often extended, because the adaptation period and logarithmic phase of microorganisms are both extended, i.e. the proliferation time is longer.

[0006] These consequences show that the process of introducing seeds into the fermentation tank needs to be operated carefully to ensure that the seed state is suitable and the culture conditions are suitable to avoid adverse effects on the fermentation process. In addition, proper seed preparation and cultivation are key to ensuring the smooth progress of the fermentation process, including selecting appropriate culture medium, controlling suitable temperature and pH value, and ensuring sufficient aeration.

[0007] Therefore, a high-efficiency cultivation of the fermentation system of high-spermidine seed of culture L-high spermidine is urgently needed to solve the problems of low fermentation efficiency and high cost. UTILITY MODEL CONTENT

[0008] In view of the above facts, the utility model discloses in order to solve the problem of low fermentation efficiency, high cost, and further designs the fermentation system of high -efficient cultivation high serine seed of culture L-high serine.

[0009] In order to achieve the above object, the utility model adopts the following technical scheme:

[0010] The fermentation system of high -efficient cultivation high serine seed of culture L-high serine, including circulating water collecting jar, agitator jar, first sewage jar, frozen water recovery main pipe, circulating water supply main pipe, frozen water supply main pipe, circulating water return main pipe, ammonia water jar, tail gas jar, seed distribution station, dilute alkali jar, second sewage jar, process wind total prefilter jar, fine filter, sterile filter, first steam jar, seed jar steam filter, waste liquid inactivation jar, second steam jar;

[0011] The left side of first pipeline is connected with circulating water collecting jar, and the right side is connected with the lower side of agitator jar, and the water drain total valve and the ice water drain valve are connected in sequence on first pipeline;

[0012] The left side of second pipeline is connected with first sewage jar, and the right side is connected with first pipeline, and the right connecting point is between water drain total valve and ice water drain valve, and circulating water blowdown valve is installed on second pipeline;

[0013] The left side of third pipeline is connected with frozen water recovery main pipe, and the right side is connected with the left side of agitator jar, and frozen water return valve is installed on third pipeline;

[0014] The left side of fourth pipeline is connected with circulating water supply main pipe, and the right side is connected with first pipeline, and the right connecting point is between ice water drain valve and agitator jar, and circulating water inlet total valve, first circulating water inlet valve and ice water regulating valve are installed in sequence on fourth pipeline;

[0015] The left side of fifth pipeline is connected with frozen water supply main pipe, and the right side is connected with fourth pipeline, and the right connecting point is between first circulating water inlet valve and ice water regulating valve, and frozen water inlet valve is installed on fifth pipeline;

[0016] The left side of sixth pipeline is connected with circulating water return main pipe, and the right side is connected with third pipeline, and the right connecting point is between frozen water return valve and agitator jar, and circulating water return total valve and second circulating water inlet valve are installed in sequence on sixth pipeline;

[0017] The left side of seventh pipeline is connected with ammonia water jar, and the right side is connected with the right side of agitator jar, and ammonia water jar valve, ammonia automatic valve and ammonia water near jar valve are installed in sequence on seventh pipeline;

[0018] The left side of eighth pipeline is connected with tail gas jar, and the right side is connected with agitator jar, and tail gas check valve and tail gas automatic valve are installed in sequence on eighth pipeline;

[0019] The ninth pipeline is connected with the seed distribution station on the left side and the stirring tank on the right side, and seed tank seed transfer valve and seed tank seed transfer valve are sequentially installed on the ninth pipeline;

[0020] The tenth pipeline is connected with the dilute alkali tank on the left side and the ninth pipeline on the right side, and the right connection point is between the seed tank seed transfer valve and the seed tank seed transfer valve, and the seed tank alkali return valve and the first seed tank blowdown valve are sequentially installed on the tenth pipeline;

[0021] The eleventh pipeline is connected with the second sewage tank on the left side and the tenth pipeline on the right side, and the right connection point is between the seed tank alkali return valve and the first seed tank blowdown valve, and the second seed tank blowdown valve is installed on the eleventh pipeline;

[0022] The twelfth pipeline is connected with the process air total pre-filter tank on the left side and the seventh pipeline on the right side, and the right connection point is between the ammonia water near tank valve and the stirring tank, and the twelfth pipeline is sequentially installed with the air inlet total valve, the air inlet tank valve, the precision filter, the filter communication valve, the sterile filter, the air inlet adjusting valve and the air inlet hand valve, and the air inlet adjusting valve is connected with the air inlet tank small exhaust on the left side below and the air inlet tank small exhaust on the right side below;

[0023] The thirteenth pipeline is connected with the first steam tank on the left side and the twelfth pipeline on the right side, and the right connection point is between the filter communication valve and the sterile filter, and the filter steam valve, the seed tank steam filter, the filter steam tank valve, the filter steam inlet tank valve and the filter steam inlet tank valve are sequentially installed on the thirteenth pipeline;

[0024] The sixteenth pipeline is connected with the thirteenth pipeline on the left side, and the left connection point is between the fifteenth pipeline left connection point and the filter steam valve, and the stirring tank is connected on the right side, and the second seed steam tank valve, the inoculation steam valve and the inoculation tank valve are sequentially installed on the sixteenth pipeline;

[0025] The eighteenth pipeline is connected with the thirteenth pipeline on the left side, and the left connection point is between the sixteenth pipeline left connection point and the filter steam valve, and the seed tank right middle part is connected on the right side, and the sampling port steam total valve, the sampling port steam valve and the sampling port tank valve are sequentially installed on the eighteenth pipeline;

[0026] The nineteenth pipeline is connected with the eighteenth pipeline on the left side, and the left connection point is between the sampling port steam valve and the sampling port tank valve, and the waste liquid inactivation tank is connected on the right side, and the sampling port discharge valve is installed on the nineteenth pipeline;

[0027] The twenty-second pipeline is connected with the second steam tank on the left side and the twenty-first pipeline on the right side, and the right connection point is between the ice water emptying valve and the twenty-first pipeline connection point, and the first jacket steam valve and the second jacket steam valve are sequentially installed on the twenty-second pipeline.

[0028] Further, the fourteenth pipeline is connected with the thirteenth pipeline on the left side, and the left connection point is between the filter steam inlet tank valve and the filter steam inlet tank valve, and the filter steam small exhaust is connected on the right side.

[0029] Further, the fifteenth pipeline is connected with the thirteenth pipeline at the left connection point between the first steam tank and the filter steam valve, and is connected with the ninth pipeline at the right connection point between the seed tank seed transfer valve and the seed transfer tank valve, and the fifteenth pipeline is sequentially provided with a first seed transfer steam far tank valve and a seed transfer steam tank valve.

[0030] Further, the seventeenth pipeline is connected with the sixteenth pipeline at the left connection point between the inoculation steam valve and the inoculation tank valve, and is connected with the inoculation blowdown valve at the right connection point.

[0031] Further, the twentieth pipeline is connected with the thirteenth pipeline at the left connection point between the eighteenth pipeline left connection point and the filter steam valve, and is connected with the twelfth pipeline at the right connection point between the air inlet adjusting valve and the air inlet hand valve, and the twentieth pipeline is sequentially provided with a main steam far tank valve and a main steam valve.

[0032] Further, the twenty-first pipeline is connected with the third pipeline at the connection point between the chilled water return valve and the stirring tank, and is provided with a chilled water blowdown valve.

[0033] The beneficial effects of the present application are as follows:

[0034] 1. The present application shortens the stagnation period of the microbial seed in the fermentation tank, advances the logarithmic growth period, reduces the production cost, and improves the production capacity of the fermentation tank.

[0035] 2. The present application can stir the microbial seed, and improve the fermentation efficiency.

[0036] 3. The present application has high heat transfer and oxygen transfer efficiency, and meets the growth demand of aerobic microorganisms.

[0037] 4. The present application has small mechanical shearing force damage to microorganisms, provides a good growth environment for microorganisms, and improves the yield and conversion rate.

[0038] 5. The present application is easy to realize large-scale and automation, has few control factors, and is convenient to operate and control. BRIEF DESCRIPTION OF DRAWINGS

[0039] Figure 1 The present application is a structural schematic diagram;

[0040] In the figure: 1-circulating water collection tank, 2-drainage total valve, 3-chilled water drainage valve, 4-stirring tank, 5-first sewage tank, 6-circulating water drainage valve, 7-chilled water recovery main pipe, 8-chilled water return valve, 9-circulating water supply main pipe, 10-circulating water inlet total valve, 11-first circulating water inlet valve, 12-chilled water regulating valve, 13-chilled water supply main pipe, 14-chilled water inlet valve, 15-circulating water return main pipe, 16-circulating water return total valve, 17-second circulating water inlet valve, 18-ammonia water tank, 19-ammonia water far tank valve, 20-ammonia automatic valve, 21-ammonia water near tank valve, 22-tail gas tank, 23-tail gas check valve, 24-tail gas automatic valve, 25-seed transfer distribution station, 26-seed tank transfer valve, 27-seed transfer near tank valve, 28-dilute alkali tank, 29-seed tank return alkali valve, 30-first seed tank drainage valve, 31-second sewage tank, 32-second seed tank drainage valve, 33-process air total pre-filter tank, 34-air inlet total valve, 35-air inlet near tank valve, 36-fine filter, 37-filter communication valve, 38-sterile filter, 39-air inlet regulating valve, 40-air inlet hand valve, 41-first steam tank, 42-filter steam valve, 43-seed tank steam filter, 44-filter steam near tank valve, 45-filter steam far tank valve, 46-filter steam near tank valve, 47-filter steam small discharge, 48-first seed transfer steam far tank valve, 49-seed transfer steam near tank valve, 50-second seed transfer steam far tank valve, 51-inoculation steam valve, 52-inoculation near tank valve, 53-inoculation drainage valve, 54-sampling port steam total valve, 55-sampling port steam valve, 56-sampling port near tank valve, 57-waste liquid inactivation tank, 58-sampling port discharge valve, 59-main steam far tank valve, 60-main steam valve, 61-chilled water discharge valve, 62-second steam tank, 63-first jacket steam valve, 64-second jacket steam valve. DETAILED DESCRIPTION

[0041] In order to make the personnel in the technical field better understand the scheme of the present application, the technical scheme in the embodiments of the present application will be clearly and completely described below in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by the person of ordinary skill in the art without creative labor should belong to the protection scope of the present application.

[0042] It should be noted that the terms "first", "second", and the like in the description and claims of the application and the above drawings are used to distinguish similar objects, and do not necessarily indicate a specific order or sequence. It should be understood that the data thus used can be interchanged under appropriate circumstances, so that the embodiments of the application described herein can be implemented. In addition, the terms "comprise" and "have" and any variations thereof are intended to cover non-exclusive inclusion, for example, a process, method, system, product or device comprising a series of steps or units does not necessarily limit to those steps or units clearly listed, but can include other steps or units not clearly listed or inherent to these processes, methods, products or devices.

[0043] In the present application, the terms "upper", "lower", "inner", "middle", "outer", "front", "back" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. These terms are mainly used to better describe the present application and its embodiments, and are not intended to limit the indicated devices, elements or components to have a specific orientation, or to be constructed and operated in a specific orientation.

[0044] In addition, in addition to being used to indicate the orientation or positional relationship, the above-mentioned partial terms can also be used to indicate other meanings, for example, the term "upper" can also be used to indicate a certain dependent relationship or connection relationship in some cases. For those skilled in the art, the specific meaning of these terms in the present application can be understood according to the specific circumstances.

[0045] In addition, the terms "set", "connected", "fixed" should be broadly understood. For example, "connected" can be fixedly connected, detachably connected, or integrally configured; can be mechanically connected, or electrically connected; can be directly connected, or indirectly connected through an intermediate medium, or internal communication between two devices, elements or components. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0046] It should be noted that the embodiments and features in the embodiments of the present application can be combined with each other without conflict. The present application will be described in detail below with reference to the accompanying drawings and in conjunction with the embodiments.

[0047] The preferred embodiments of the present application will be described in detail below with reference to the accompanying drawings.

[0048] Embodiment 1: The fermentation system for culturing L-homoserine of high-efficiency homoserine seed culture in this embodiment comprises: a circulating water collecting tank 1, a stirring tank 4, a first sewage tank 5, a chilled water recovery main pipe 7, a circulating water supply main pipe 9, a chilled water supply main pipe 13, a circulating water return main pipe 15, an ammonia water tank 18, a tail gas tank 22, a seed transfer distribution station 25, a dilute alkali tank 28, a second sewage tank 31, a process air total pre-filter tank 33, a precision filter 36, a sterile filter 38, a first steam tank 41, a seed tank steam filter 43, a waste liquid inactivation tank 57, and a second steam tank 62.

[0049] The first pipe is connected to the circulating water collecting tank 1 on the left side and connected to the lower part of the stirring tank 4 on the right side, and the water drainage total valve 2 and the chilled water drainage valve 3 are sequentially connected to the upper part of the first pipe.

[0050] The second pipe is connected to the first sewage tank 5 on the left side and connected to the first pipe on the right side, and the circulating water drainage valve 6 is installed on the upper part of the second pipe.

[0051] The third pipe is connected to the chilled water recovery main pipe 7 on the left side and connected to the left side of the stirring tank 4 on the right side, and the chilled water return valve 8 is installed on the upper part of the third pipe.

[0052] The fourth pipe is connected to the circulating water supply main pipe 9 on the left side and connected to the first pipe on the right side, and the circulating water inlet total valve 10, the first circulating water inlet valve 11, and the chilled water adjusting valve 12 are sequentially installed on the upper part of the fourth pipe.

[0053] The fifth pipe is connected to the chilled water supply main pipe 13 on the left side and connected to the fourth pipe on the right side, and the chilled water inlet valve 14 is installed on the upper part of the fifth pipe.

[0054] The sixth pipe is connected to the circulating water return main pipe 15 on the left side and connected to the third pipe on the right side, and the circulating water return total valve 16 and the second circulating water inlet valve 17 are sequentially installed on the upper part of the sixth pipe.

[0055] The seventh pipe is connected to the ammonia water tank 18 on the left side and connected to the right side of the stirring tank 4 on the right side, and the ammonia water far tank valve 19, the ammonia automatic valve 20, and the ammonia water near tank valve 21 are sequentially installed on the seventh pipe.

[0056] The eighth pipe is connected to the tail gas tank 22 on the left side and connected to the stirring tank 4 on the right side, and the tail gas check valve 23 and the tail gas automatic valve 24 are sequentially installed on the eighth pipe.

[0057] The ninth pipe is connected to the seed transfer distribution station 25 on the left side and connected to the stirring tank 4 on the right side, and the seed tank seed transfer valve 26 and the seed transfer near tank valve 27 are sequentially installed on the ninth pipe.

[0058] The tenth pipeline is connected with the dilute alkali tank 28 on the left side and the ninth pipeline on the right side, and the right connection point is between the seed tank seed transfer valve 26 and the seed tank seed transfer tank valve 27, and the tenth pipeline is sequentially provided with the seed tank alkali return valve 29 and the first seed tank sewage valve 30;

[0059] The eleventh pipeline is connected with the second sewage tank 31 on the left side and the tenth pipeline on the right side, and the right connection point is between the seed tank alkali return valve 29 and the first seed tank sewage valve 30, and the eleventh pipeline is sequentially provided with the second seed tank sewage valve 32;

[0060] The twelfth pipeline is connected with the process air total pre-filter tank 33 on the left side and the seventh pipeline on the right side, and the right connection point is between the ammonia water near tank valve 21 and the stirring tank 4, and the twelfth pipeline is sequentially provided with the air inlet total valve 34, the air inlet tank valve 35, the precision filter 36, the filter communication valve 37, the sterile filter 38, the air inlet adjusting valve 39 and the air inlet hand valve 40, and the air inlet adjusting valve 39 is connected with the air inlet tank small exhaust on the left side below and the air inlet tank small exhaust on the right side below;

[0061] The thirteenth pipeline is connected with the first steam tank 41 on the left side and the twelfth pipeline on the right side, and the right connection point is between the filter communication valve 37 and the sterile filter 38, and the thirteenth pipeline is sequentially provided with the filter steam valve 42, the seed tank steam filter 43, the filter steam tank valve 44, the filter steam inlet tank valve 45 and the filter steam inlet tank valve 46;

[0062] The sixteenth pipeline is connected with the thirteenth pipeline on the left side, and the left connection point is between the fifteenth pipeline left connection point and the filter steam valve 42, and the right side is connected with the stirring tank 4, and the sixteenth pipeline is sequentially provided with the second seed transfer steam tank valve 50, the inoculation steam valve 51 and the inoculation tank valve 52;

[0063] The eighteenth pipeline is connected with the thirteenth pipeline on the left side, and the left connection point is between the sixteenth pipeline left connection point and the filter steam valve 42, and the right side is connected with the middle part of the stirring tank 4 on the right side, and the eighteenth pipeline is sequentially provided with the sampling port steam total valve 54, the sampling port steam valve 55 and the sampling port tank valve 56;

[0064] The nineteenth pipeline is connected with the eighteenth pipeline on the left side, and the left connection point is between the sampling port steam valve 55 and the sampling port tank valve 56, and the right side is connected with the waste liquid inactivation tank 57, and the nineteenth pipeline is provided with the sampling port discharge valve 58;

[0065] The twenty-second pipeline is connected with the second steam tank 62 on the left side and the twenty-first pipeline on the right side, and the right connection point is between the ice water exhaust valve 61 and the twenty-first pipeline connection point, and the twenty-second pipeline is sequentially provided with the first jacket steam valve 63 and the second jacket steam valve 64.

[0066] More specifically: the fourteenth pipeline left side connects the thirteenth pipeline, left connection point between filter steam far tank valve 45 and filter steam close tank valve 46, right side connects filter steam small discharge 47.

[0067] More specifically: the fifteenth pipeline left side connects the thirteenth pipeline, left connection point between first steam tank 41 and filter steam valve 42, right side connects the ninth pipeline, right side connection point between seed tank seedling valve 26 and seedling close tank valve 27, the fifteenth pipeline is sequentially installed first seedling steam far tank valve 48 and seedling steam close tank valve 49.

[0068] More specifically: the seventeenth pipeline left side connects the sixteenth pipeline, left connection point between inoculation steam valve 51 and inoculation close tank valve 52, right side connects inoculation blowdown valve 53.

[0069] More specifically: the twentieth pipeline left side connects the thirteenth pipeline, left connection point between the left connection point of the eighteenth pipeline and filter steam valve 42, right side connects the twelfth pipeline, right connection point between air inlet regulating valve 39 and air inlet hand valve 40, the twentieth pipeline is sequentially connected with main steam far tank valve 59 and main steam valve 60.

[0070] More specifically: the twenty-first pipeline connects the third pipeline, connection point between chilled water return valve 8 and stirring tank 4, and the twenty-first pipeline is installed with chilled water blowdown valve 61.

[0071] Embodiment 2: the fermentation system of the present embodiment for culturing L-homoserine with high-efficiency homoserine seed cultivation, the operation process of the seed tank is as follows:

[0072] Step 1, seed tank inactivation, valve inspection, leak detection;

[0073] Step 2, seed tank alkali boiling, alkali disinfection;

[0074] Step 3, seed tank empty disinfection;

[0075] Step 4, seed tank ingredient preparation;

[0076] Step 5, seed tank real tank sterilization;

[0077] Step 6, inoculation;

[0078] Step 7, culture;

[0079] Step 8, seed tank to fermentation tank seedling.

[0080] More specifically: the specific implementation process of step 1 is as follows:

[0081] A, leak detection: after the seedling is finished, the pressure does not decrease, and the pressure is raised to 0.15 MPa, and the shaft seal and all near-tank small discharges and valves are tested for leakage, and the leakage points are treated;

[0082] B. Temperature increase inactivation: the circulating water inlet and outlet is closed, the upper and lower exhaust of the coil is fully opened, the temperature is increased to 80 degrees or more, and the steam inactivation is carried out through the opening of each near tank small exhaust;

[0083] C. Cleaning the shaft seal: use water to clean the materials and oil around the shaft seal;

[0084] D. Tank flushing: open the water valve for cleaning when the pressure is reduced to 0;

[0085] E. When checking the tank, all tank valves are removed and inspected. The valve removal and inspection process is as follows:

[0086] E1. Safety inspection: after the inoculation is completed, close the air inlet valve, open the exhaust valve, remove the air in the tank, reduce the tank pressure to 0 MPa, and ensure that there is no pressure in the tank before removing the valve. When removing the valve, the valve head is taken out sideways;

[0087] E2. Cleaning inspection: wash the inside of the valve cavity with water, there should be no material, check if the sealing surface in the valve cavity is damaged, if damaged, report in time to consider whether to replace the valve, and if the valve pad indentation is too deep, the valve head pad should be replaced;

[0088] E3. Installation: check the valve port pad indentation and consider whether to replace it; when installing, start tightening the screws diagonally after the valve is opened to prevent leakage due to pressure deviation;

[0089] E4. Inspection: check if the valve parts are missing, and perform opening and closing tests after the valve installation is completed. The tank is pressure tested for leakage, the pressure is increased to 0.15 MPa, and the valve is checked for "running, leaking, dripping, and leaking" phenomena;

[0090] E5. End of work: after the valve removal and inspection is completed, put the tools used back into the tool box, clean the old pads and garbage generated during the valve removal and inspection, pour the water used for valve removal and inspection into the water pool, wash the water bucket and put it back to its original place, spray disinfectant around the valve to prevent some valves from leaking during the removal and inspection process.

[0091] More specifically: the specific implementation process of step 2 is as follows:

[0092] A. Pull out the valve inspection electrode, and then press and discharge the tank flushing water in the tank;

[0093] B. Close the water inlet and return water valves, open the upper and lower exhaust valves of the coil, and start the alkali inlet pump;

[0094] C. Open the stirring, control the alkali liquid level, and after the alkali tank is heated to 90 degrees, close the valve, and after 2 hours of alkali boiling, return the alkali;

[0095] D. After the alkali water is pressed, the tank pressure is reduced to 0, the water inlet valve is opened for flushing, the sewage valve is opened, and the sewage in the tank is discharged.

[0096] More specifically: the specific implementation process of step 3 is:

[0097] A, pressure maintaining by passing ammonia: the automatic ammonia supplement valve is manually opened to pass ammonia into the tank;

[0098] B, open the air distribution ring steam valve, open the seed transfer valve and sampling valve, fully open the exhaust valve, close the seed transfer to distribution disc valve, sequentially open each small exhaust valve on the tank body, remove the cold air in the tank, when the temperature rises to 90 degrees, close the small exhaust valve, at this time the cold air in the tank has been removed and the temperature rises rapidly;

[0099] C, loosen the screw cap of the inoculation port and start steam exhaust;

[0100] D, close the small exhaust valve when the exhaust steam is blue;

[0101] E, slowly raise the temperature to 123 degrees, the pressure is about 0.12 MPa, and the time is 0.5 hours;

[0102] F, end of air blowing, sequentially close the opened small exhaust, close the steam inlet valve, open the blowdown valve, and exhaust the condensate in the tank;

[0103] G, record: cook alkali (time, temperature, pH), check valve position, air blowing pressure, temperature, time, etc., and record on the report.

[0104] More specifically: the specific implementation process of step 4 is:

[0105] A, weighing

[0106] A1, calibration: prepare the loading container, calibrate the weighing instrument and clean it;

[0107] A2, weighing: weigh the raw materials according to the medium ratio and usage, and record for reference;

[0108] A3, review: review according to the ingredient list, and add materials after no error;

[0109] B, ingredient

[0110] B1, check: check the valve and pipeline of the ingredient tank to ensure they are in the correct position, and then add materials after cleaning;

[0111] B2, add materials: add materials according to the formula in sequence and with accurate weight;

[0112] B3, mix materials: check the valves of the seed tank to ensure they are in the correct position, and then pump the materials to the first-stage seed tank;

[0113] B4, volume setting: 1m 3 mixed materials + 0.8m 3 real blowing condensate + 0.2m 3 bottom sugar ≈ 2.0m3

[0114] B5, after the end of the constant volume, open the stirring, put in the dissolved trace elements, defoaming agent, after the end of the preparation, clean and store each container.

[0115] More specifically: the specific implementation process of step 5 is:

[0116] A, preheating

[0117] A1, stirring: open the seed tank stirring;

[0118] A2, steam: open the jacket steam, and use the jacket to heat the material in the tank for preheating;

[0119] A3: temperature rise: preheat to 90 degrees, open the exhaust valve before 90 degrees, so as to exhaust the cold air in the tank and prevent the formation of false pressure during the tank process;

[0120] B, three-way air inlet:

[0121] B1, preheat the liquid to 90 degrees, close the small exhaust valve, open the main steam, open the steam pipeline, take the sample port into the steam, open the near tank valve and each small exhaust, unscrew the inoculation port nut, start to increase the pressure, and the steam is mainly air pipeline steam. Too small steam flow will cause liquid backflow;

[0122] B2, temperature rises to 108 degrees, close the jacket steam, and use the remaining three-way steam to control the temperature to rise smoothly;

[0123] B3, tank pressure control 0.1-0.12MPa, temperature 121 degrees, maintain for 20 minutes;

[0124] C, cooling

[0125] C1, stop steam: close the inoculation cap, close all near tank valves on the tank top, and close all steam pressure in the triangular area on the tank top. Finally, close the main steam of the air pipeline;

[0126] C2, air pressure: adjust the exhaust valve, and when the tank pressure is lower than the air pressure of the filter, immediately introduce sterile air to maintain pressure. The pressure is 0.04MPa, and the air volume is 2m 3 / h;

[0127] C3, cooling: after the pressure is stable, first open the circulating water outlet valve, then slowly open the circulating water inlet valve, and finally open the valve. First, change the air, then cool down, so as to prevent negative pressure in the tank. After the sterilization, open the main steam before closing to top up the remaining material in the pipeline into the tank, so as to avoid incomplete dyeing caused by dyeing.

[0128] More specifically: the specific implementation process of step 6 is:

[0129] A, Preparation: adjust the pH of the electrode with ammonia water before inoculation, adjust the tank temperature to 30 degrees, the tank pressure is 0.04 MPa, the air flow is 2 m 3 / h, stirring 100%, DO 100% calibration; and check the pre-inoculation indicators PH, DO, RG are normal;

[0130] B, Take sterile sample: open the steam of the large seed tank sampling port before inoculation, sterilize for 10-15 minutes, and take sample;

[0131] C, Environmental sterilization: spray disinfectant around the seed tank for sterilization;

[0132] D, Inoculation preparation: stop stirring, reduce air flow and personnel movement, press the inoculation bottle with protective equipment, and the inoculator disinfects with 75% alcohol;

[0133] E, Inoculation port disinfection: pour 95% alcohol on cotton balls and use outer flame to burn the inoculation tube port;

[0134] F, Rotate the nut on the inoculation tube port under the protection of the flame;

[0135] G, Inoculation bottle: remove the gauze on the inoculation bottle port, and quickly insert the rubber tube into the inoculation port above the flame;

[0136] H, Inoculation liquid: stabilize the tank pressure at 0.04 MPa, open the inoculation valve, slowly open the exhaust valve, and inject the bacterial suspension into the seed tank with the help of pressure difference;

[0137] I, After inoculation, close the inoculation valve tightly, still seal with flame, remove the rubber tube, and tighten the sterilized nut;

[0138] J, After inoculation, sterilize the sampling port with steam for 10-15 minutes, take 0 hour sterile sample and post-inoculation test sample.

[0139] More specifically: the specific implementation process of step 7 is:

[0140] A, Adjust the tank pressure: the tank pressure is controlled at 0.04 MPa, and is observed and adjusted at any time. The tank pressure data value is set in the main control room, and remote automatic control is adopted. The post personnel opens the air inlet valve, and the main control room controls the pressure in the tank remotely through automatic valve. When the pressure is higher or lower than 0.04 MPa, the automatic valve will automatically open or close under remote control to ensure the stability of the tank pressure;

[0141] B, Adjust the air flow: control and adjust the air flow in time according to the process requirements, and control the air flow at 2 m 3 / h, the method is the same as controlling the tank pressure;

[0142] C. Temperature control: temperature control 30 degrees, at any time to observe, adjust, master control room using remote automation control, post personnel will be into the circulating water hand valve open, master control room in remote through the automatic valve control tank temperature, that is, higher or lower than 30 ℃, the automatic valve will be in remote control automatically open or close, to ensure the stability of the tank temperature;

[0143] D, each sampling pH, and make a good record, the control of pH using ammonia automatic valve and pH electrode interlocking, when the tank pH value is lower than the set value, ammonia automatic valve opens, when the tank pH value is lower than the set value 0.05, automatic closing, to avoid excessive ammonia water into the amount of tank pH value is high, the impact of fermentation.

[0144] More specifically: the specific implementation process of step 8 is:

[0145] A, seed tank culture 6 hours, open the seed transfer pipeline steam to seed tank to fermentation tank seed transfer pipeline disinfection;

[0146] B, the seed transfer pipeline pressure retention first seed tank to fermentation tank seed transfer pipeline and distribution plate on all the small row closed, seed transfer steam valve closed, finally quickly open the inoculation valve on the fermentation tank, using the sterile air in the fermentation tank to keep the pressure of the seed transfer pipeline;

[0147] C, when the OD value to the process requirements, seed tank ammonia valve closed, stop stirring, open the seed valve, then close the exhaust, open the inlet to maintain the tank pressure at 0.1 Mpa, seed liquid in the role of differential pressure from seed tank through the seed transfer pipeline into the fermentation tank

[0148] D, when the seed liquid is removed, quickly close the inoculation valve on the fermentation tank, then close the seed tank seed, close the inlet, start seed transfer equipment leak test.

[0149] More specifically: the process flow of seed tank: breeding-seed tank

[0150] Process flow introduction: through the breeding room for breeding, when the strain OD value reaches the requirements of production, end of breeding, breeding staff will seed into the seed tank which has been inoculated with the conditions, under the control of process requirements, the strain culture control, when the seed tank nutrient consumption, through sampling analysis, to meet the requirements of the fermentation tank inoculation, seed to the fermentation tank.

[0151] More specifically: introduction of each post responsibility:

[0152] Breeding: breeding of homoserine strain, to ensure that the seed is in the best state (enough number and vigorous activity of seed free of bacteria) inoculated into the seed tank.

[0153] Seed tank: the purpose of the seed tank is to provide a sufficient number and vigorous bacteria-free seeds for the fermenter, to ensure that the fermenter enters the logarithmic growth phase in the shortest time, to reduce the cost of culture, and to improve the single-tank capacity.

[0154] More specifically: the seed index is 7 hours of bacterial age, net OD 12 immediate seed transfer, microscopic examination, healthy bacteria, uniform arrangement, no contamination.

[0155] More specifically: during the process, the pressure, temperature, temperature rising time, pressure holding time, operator, seed tank culture time, temperature, pH, OD, RG, air volume, and speed of seed tank are recorded, and the seed tank is boiled, tank inspection, valve inspection, ingredient, and review.

[0156] More specifically: overpressure is strictly prohibited during the emptying process, and people are strictly prohibited near the tank opening.

[0157] More specifically: the allowable range of weighing error is ±0.01.

[0158] More specifically: negative pressure is strictly prohibited during feeding.

[0159] More specifically: each steam inlet should be unobstructed, and the existence of dead angles should be strictly prevented.

[0160] More specifically: the steam pressure in the tank should be lower than the air pressure of the filter to open the air pressure, otherwise it is easy to cause backflow of the liquid.

[0161] More specifically: the tank temperature, PH, OD, RG must be detected before inoculation, and the inoculation can be done only after the detection is correct.

[0162] More specifically: during the inoculation process, the tank pressure is not allowed to drop to 0 pressure, and when the tank pressure drops to 0.03MPa, it can be raised to 0.04MPa again for re-inoculation (which can be repeated several times until the seed is inoculated).

[0163] More specifically: when sampling, do not let the liquid splash out of the blowdown tank, and disinfect it in time after each sampling.

[0164] More specifically: the high serine seed tank is based on the principle of microbial fermentation. After the bacteria are bred in the breeding room, the breeder inoculates the seed into the seed tank that has met the inoculation conditions through differential pressure inoculation. The stirring needs to be temporarily stopped during inoculation, the temperature of the seed tank is controlled at 30 degrees, the pH value is controlled at 7, and the seed tank is controlled at 0.4㎏ / cm 2 The seed tank dissolved oxygen is controlled between 25-30, and the bacteria are cultured in the seed tank for about 7 hours. That is, the nutrient components in the seed tank are depleted, and the OD value meets the inoculation requirements of the fermenter.

[0165] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application, and are not limiting; although the present application has been described in detail with reference to the foregoing examples, the technical solutions described in the foregoing examples can still be modified, or some or all of the technical features can be replaced by equivalents; as long as there is no structural conflict, each feature in the specific embodiments disclosed in the present application can be used in combination with any other feature, and will not cause the corresponding technical solution to deviate from the scope of the technical solutions of the present application.

[0166] In addition, it should be understood that, although the present specification is described in terms of embodiments, not every embodiment contains only one independent technical solution, and the description of the specification is only for the sake of clarity, and those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that those skilled in the art can understand.

Claims

1. A fermentation system for the efficient cultivation of L-homoserine seeds, characterized in that, Includes a circulating water collection tank (1), a mixing tank (4), a first sewage tank (5), a chilled water recovery main pipe (7), a circulating water supply main pipe (9), a chilled water supply main pipe (13), a circulating water return main pipe (15), an ammonia tank (18), a tail gas tank (22), a seed distribution station (25), a dilute alkali tank (28), a second sewage tank (31), a process air pre-filter tank (33), a fine filter (36), a sterile filter (38), a first steam tank (41), a seed tank steam filter (43), a waste liquid inactivation tank (57), and a second steam tank (62); The first pipe is connected to the circulating water collection tank (1) on the left and to the bottom of the mixing tank (4) on the right. The main drain valve (2) and the ice water drain valve (3) are connected to the first pipe in sequence. The second pipe is connected to the first sewage tank (5) on the left and to the first pipe on the right. The right connection point is between the main drain valve (2) and the ice water drain valve (3). A circulating water drain valve (6) is installed on the second pipe. The third pipe is connected to the chilled water recovery main pipe (7) on the left and to the left side of the mixing tank (4) on the right. A chilled water return valve (8) is installed on the third pipe. The fourth pipe is connected to the circulating water supply main pipe (9) on the left and to the first pipe on the right. The right connection point is between the ice water drain valve (3) and the mixing tank (4). The circulating water inlet main valve (10), the first circulating water inlet valve (11), and the ice water regulating valve (12) are installed on the fourth pipe in sequence. The fifth pipe is connected to the chilled water supply main pipe (13) on the left and to the fourth pipe on the right. The right connection point is between the first circulating water inlet valve (11) and the chilled water regulating valve (12). The fifth pipe is equipped with a chilled water inlet valve (14). The sixth pipe is connected to the circulating water return main pipe (15) on the left and to the third pipe on the right. The right connection point is between the chilled water return valve (8) and the mixing tank (4). The circulating water return main valve (16) and the second circulating water inlet valve (17) are installed on the sixth pipe in sequence. The seventh pipeline is connected to the ammonia tank (18) on the left and to the right of the mixing tank (4) on the right. The seventh pipeline is equipped with the ammonia remote tank valve (19), the ammonia automatic valve (20), and the ammonia near tank valve (21) in sequence. The eighth pipeline is connected to the tail gas tank (22) on the left and the mixing tank (4) on the right. The tail gas check valve (23) and the tail gas automatic valve (24) are installed in sequence on the eighth pipeline. The ninth pipeline is connected to the seed distribution station (25) on the left and to the mixing tank (4) on the right. The seed tank transfer valve (26) and the seed tank transfer valve (27) are installed in sequence on the ninth pipeline. The tenth pipeline is connected to the dilute alkali tank (28) on the left and to the ninth pipeline on the right. The right connection point is between the seed tank transfer valve (26) and the transfer tank valve (27). The tenth pipeline is installed with the seed tank return alkali valve (29) and the first seed tank drain valve (30) in sequence. The eleventh pipeline is connected to the second sewage tank (31) on the left and to the tenth pipeline on the right. The right connection point is between the seed tank alkali return valve (29) and the first seed tank drain valve (30). The eleventh pipeline is equipped with the second seed tank drain valve (32). The twelfth pipeline is connected to the process air pre-filter tank (33) on the left and to the seventh pipeline on the right. The right connection point is between the ammonia water near-tank valve (21) and the mixing tank (4). The twelfth pipeline is installed with the main air inlet valve (34), the air inlet near-tank valve (35), the fine filter (36), the filter connecting valve (37), the sterile filter (38), the air inlet regulating valve (39), and the air inlet manual valve (40) in sequence. The air inlet regulating valve (39) is connected to the air inlet near-tank drain on the left and to the air inlet far-tank drain on the right. The thirteenth pipeline is connected to the first steam tank (41) on the left and to the twelfth pipeline on the right. The right connection point is between the filter connecting valve (37) and the sterile filter (38). The thirteenth pipeline is installed with the filter steam valve (42), the seed tank steam filter (43), the filter steam near the tank valve (44), the filter steam far from the tank valve (45), and the filter steam near the tank valve (46) in sequence. The sixteenth pipe is connected to the thirteenth pipe on the left side, and the left connection point is between the left connection point of the fifteenth pipe and the filter steam valve (42). The right side is connected to the mixing tank (4). The sixteenth pipe is installed with the second transfer steam remote tank valve (50), the inoculation steam valve (51), and the inoculation tank valve (52) in sequence. The eighteenth pipe is connected to the thirteenth pipe on the left side. The left connection point is between the left connection point of the sixteenth pipe and the filter steam valve (42). The right side is connected to the middle of the right side of the mixing tank (4). The eighteenth pipe is installed with the sampling port steam main valve (54), the sampling port steam valve (55), and the sampling port tank valve (56) in sequence. The nineteenth pipeline is connected to the eighteenth pipeline on the left side. The left connection point is between the sampling port steam valve (55) and the sampling port tank valve (56). The right side is connected to the waste liquid inactivation tank (57). A sampling port discharge valve (58) is installed on the nineteenth pipeline. The 22nd pipeline is connected to the second steam tank (62) on the left and to the 21st pipeline on the right. The right connection point is between the ice water vent valve (61) and the connection point of the 21st pipeline. The 22nd pipeline is equipped with the first jacketed steam valve (63) and the second jacketed steam valve (64) in sequence.

2. The fermentation system for high-efficiency cultivation of L-homoserine seeds according to claim 1, characterized in that: The fourteenth pipe is connected to the thirteenth pipe on the left. The left connection point is between the filter steam inlet remote valve (45) and the filter steam inlet near valve (46). The right side is connected to the filter steam drain (47).

3. The fermentation system for high-efficiency cultivation of L-homoserine seeds according to claim 1, characterized in that: The fifteenth pipe is connected to the thirteenth pipe on the left, with the left connection point between the first steam tank (41) and the filter steam valve (42). The right pipe is connected to the ninth pipe, with the right connection point between the seed tank transfer valve (26) and the transfer tank valve (27). The fifteenth pipe is installed with the first transfer steam remote tank valve (48) and the transfer steam near tank valve (49) in sequence.

4. The fermentation system for high-efficiency cultivation of L-homoserine seeds according to claim 1, characterized in that: The seventeenth pipe is connected to the sixteenth pipe on the left side, with the left connection point between the inoculation steam valve (51) and the inoculation tank valve (52), and the right side is connected to the inoculation drain valve (53).

5. The fermentation system for efficiently cultivating L-homoserine seeds according to claim 1, characterized in that: The 20th pipe connects to the 13th pipe on the left, with the left connection point between the left connection point of the 18th pipe and the filter steam valve (42). The 20th pipe connects to the 12th pipe on the right, with the right connection point between the air inlet regulating valve (39) and the air inlet manual valve (40). The 20th pipe connects to the main steam remote tank valve (59) and the main steam valve (60) in sequence.

6. The fermentation system for efficiently cultivating L-homoserine seeds according to claim 1, characterized in that: Pipeline 21 connects to pipe 3, with the connection point between chilled water return valve (8) and mixing tank (4). Pipeline 21 is equipped with chilled water drain valve (61).

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